1. Characterisation of a non-pathogenic and non-protective infectious rabbit lagovirus related to RHDV
- Author
-
Jacqueline Gelfi, Giuliana Botti, Ghislaine Le Gall-Reculé, Alain Roobrouck, F. Zwingelstein, Marie-Philippe Fages, Antonio Lavazza, Stéphane Bertagnoli, Jacky Aubineau, Stéphane Marchandeau, Agence Nationale de Sécurité Sanitaire - ANSES (FRANCE), Ecole Nationale Vétérinaire de Toulouse - ENVT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Instituto Zooprofilattico Sperimentale della Lombardia et dell' Emilia Romagna - IZSLER (ITALY), Office National de la Chasse et de la Faune Sauvage - ONCFS (FRANCE), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Office National de la Chasse et de la Faune Sauvage (ONCFS), and Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna 'Bruno Ubertini' (IZSLER)
- Subjects
Hemorrhagic Disease Virus, Rabbit ,040301 veterinary sciences ,Sequence analysis ,Cross Protection ,[SDV]Life Sciences [q-bio] ,Molecular Sequence Data ,Sequence Homology ,Cuniculus ,Rabbit ,Antibodies, Viral ,Bunyaviridae Infections ,Virus ,0403 veterinary science ,03 medical and health sciences ,Phylogenetics ,RHVD ,Virology ,Calicivirus ,Animals ,Cluster Analysis ,Phylogeny ,030304 developmental biology ,0303 health sciences ,biology ,Phylogenetic tree ,Lagovirus ,Non-pathogenic virus ,RHDV ,Sequence Analysis, DNA ,04 agricultural and veterinary sciences ,biology.organism_classification ,3. Good health ,Médecine vétérinaire et santé animal ,Capsid ,Carrier State ,Tissue tropism ,RNA, Viral ,RNA ,Oryctolagus ,Rabbits - Abstract
International audience; The existence of non-pathogenic RHDV strains was established when a non-lethal virus named rabbit calicivirus (RCV) was characterised in 1996 in Italy. Since then, different RNA sequences related to RHDV have been detected in apparently healthy domestic and wild rabbits, and recently a new lagovirus was identified in Australia. We have characterised from seropositive healthy domestic rabbits a non-lethal lagovirus that differs from RHDV in terms of pathogenicity, tissue tropism and capsid protein sequence. Phylogenetic analyses have revealed that it is close to the Ashington strain and to the RCV, but distinct. We proved experimentally that it is infectious but non-pathogenic and demonstrated that, contrary to the other described non-pathogenic lagoviruses, it induces antibodies that do not protect against RHDV. Our results indicate the existence of a gradient of cross-protection between circulating strains, from non-protective, partially protective to protective strains, and highlight the extent of diversity within the genus Lagovirus.
- Published
- 2011
- Full Text
- View/download PDF