Search

Your search keyword '"Ohba, Yusuke"' showing total 696 results
Start Over Author "Ohba, Yusuke"
696 results on '"Ohba, Yusuke"'

2. Comparative pathogenicity of SARS-CoV-2 Omicron subvariants including BA.1, BA.2, and BA.5

Author

Tamura, Tomokazu, Yamasoba, Daichi, Oda, Yoshitaka, Ito, Jumpei, Kamasaki, Tomoko, Nao, Naganori, Hashimoto, Rina, Fujioka, Yoichiro, Suzuki, Rigel, Wang, Lei, Ito, Hayato, Kashima, Yukie, Kimura, Izumi, Kishimoto, Mai, Tsuda, Masumi, Sawa, Hirofumi, Yoshimatsu, Kumiko, Yamamoto, Yuki, Nagamoto, Tetsuharu, Kanamune, Jun, Suzuki, Yutaka, Ohba, Yusuke, Yokota, Isao, Matsuno, Keita, Takayama, Kazuo, Tanaka, Shinya, Sato, Kei, and Fukuhara, Takasuke

Published
2023
Full Text
View/download PDF

6. At2-MMP Is Required for Attenuation of Cell Proliferation during Wound Healing in Incised Arabidopsis Inflorescence Stems.

Author

Machfuudzoh, Afiifah, Pitaksaringkarn, Weerasak, Koshiba, Ryo, Higaki, Takumi, Rakwal, Randeep, Ohba, Yusuke, Asahina, Masashi, Satoh, Shinobu, and Iwai, Hiroaki

Abstract

Wound healing of partially incised Arabidopsis inflorescence stems constitutes cell proliferation that initiates mainly in pith tissues about 3 d after incision and the healing process that completes in about 7 d. Although the initiation mechanisms of cell proliferation have been well documented, the suppression mechanisms remain elusive. Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases well known as proteolytic enzymes in animal system functioning in extracellular matrix remodeling during physiological and pathological processes, including tissue differentiation, growth, defense, wound healing and control of cancer growth. In this study, we report that At2-MMP might contribute to the suppression mechanism for cell proliferation during the tissue-repair process of incised inflorescence stems. At2-MMP transcript was gradually upregulated from day 0 to 5 after incision and slightly decreased on day 7. Morphological analysis of incised stem of defected mutant at2-mmp revealed significantly enhanced cell proliferation around the incision site. Consistent with this, semi-quantitative analysis of dividing cells displayed a significant increment in the number of dividing cells in at2-mmp as compared to wild type. These results showed that the upregulation of At2-MMP at a later stage of the wound-healing process is likely to be involved in the completion of the process by attenuating cell proliferation. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

7. Calcium Wave Promotes Cell Extrusion

Author

Takeuchi, Yasuto, Narumi, Rika, Akiyama, Ryutaro, Vitiello, Elisa, Shirai, Takanobu, Tanimura, Nobuyuki, Kuromiya, Keisuke, Ishikawa, Susumu, Kajita, Mihoko, Tada, Masazumi, Haraoka, Yukinari, Akieda, Yuki, Ishitani, Tohru, Fujioka, Yoichiro, Ohba, Yusuke, Yamada, Sohei, Hosokawa, Yoichiroh, Toyama, Yusuke, Matsui, Takaaki, and Fujita, Yasuyuki

Published
2020
Full Text
View/download PDF

11. Discovery of Novel NLRP3 Inflammasome Inhibitors Composed of an Oxazole Scaffold Bearing an Acylsulfamide

Author

Ohba, Yusuke, primary, Adachi, Kaoru, additional, Furukawa, Takayuki, additional, Nishimaru, Tatsuya, additional, Sakurai, Kentaro, additional, Masuo, Ritsuki, additional, Inami, Tasuku, additional, Orita, Takuya, additional, Akai, Shota, additional, Adachi, Tsuyoshi, additional, Usui, Kenji, additional, Hamada, Yuji, additional, Mori, Mutsuki, additional, Kurimoto, Takafumi, additional, Wakashima, Takeshi, additional, Akiyama, Yoshiyuki, additional, Miyazaki, Susumu, additional, and Noji, Satoru, additional

Published
2023
Full Text
View/download PDF

20. Intravenous Administration of Mesenchymal Stem Cell-Derived Exosome Alleviates Spinal Cord Injury by Regulating Neutrophil Extracellular Trap Formation through Exosomal miR-125a-3p.

Author

Morishima, Yutaka, Kawabori, Masahito, Yamazaki, Kazuyoshi, Takamiya, Soichiro, Yamaguchi, Sho, Nakahara, Yo, Senjo, Hajime, Hashimoto, Daigo, Masuda, Sakiko, Fujioka, Yoichiro, Ohba, Yusuke, Mizuno, Yuki, Kuge, Yuji, and Fujimura, Miki

Subjects
NEUTROPHILS, SPINAL cord injuries, INTRAVENOUS therapy, POSITRON emission tomography, MESENCHYMAL stem cells, EXOSOMES
Abstract

Spinal cord injury (SCI) leads to devastating sequelae, demanding effective treatments. Recent advancements have unveiled the role of neutrophil extracellular traps (NETs) produced by infiltrated neutrophils in exacerbating secondary inflammation after SCI, making it a potential target for treatment intervention. Previous research has established that intravenous administration of stem cell-derived exosomes can mitigate injuries. While stem cell-derived exosomes have demonstrated the ability to modulate microglial reactions and enhance blood–brain barrier integrity, their impact on neutrophil deactivation, especially in the context of NETs, remains poorly understood. This study aims to investigate the effects of intravenous administration of MSC-derived exosomes, with a specific focus on NET formation, and to elucidate the associated molecular mechanisms. Exosomes were isolated from the cell supernatants of amnion-derived mesenchymal stem cells using the ultracentrifugation method. Spinal cord injuries were induced in Sprague-Dawley rats (9 weeks old) using a clip injury model, and 100 μg of exosomes in 1 mL of PBS or PBS alone were intravenously administered 24 h post-injury. Motor function was assessed serially for up to 28 days following the injury. On Day 3 and Day 28, spinal cord specimens were analyzed to evaluate the extent of injury and the formation of NETs. Flow cytometry was employed to examine the formation of circulating neutrophil NETs. Exogenous miRNA was electroporated into neutrophil to evaluate the effect of inflammatory NET formation. Finally, the biodistribution of exosomes was assessed using 64Cu-labeled exosomes in animal positron emission tomography (PET). Rats treated with exosomes exhibited a substantial improvement in motor function recovery and a reduction in injury size. Notably, there was a significant decrease in neutrophil infiltration and NET formation within the spinal cord, as well as a reduction in neutrophils forming NETs in the circulation. In vitro investigations indicated that exosomes accumulated in the vicinity of the nuclei of activated neutrophils, and neutrophils electroporated with the miR-125a-3p mimic exhibited a significantly diminished NET formation, while miR-125a-3p inhibitor reversed the effect. PET studies revealed that, although the majority of the transplanted exosomes were sequestered in the liver and spleen, a notably high quantity of exosomes was detected in the damaged spinal cord when compared to normal rats. MSC-derived exosomes play a pivotal role in alleviating spinal cord injury, in part through the deactivation of NET formation via miR-125a-3p. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

27. Comparative pathogenicity of SARS-CoV-2 Omicron subvariants including BA.1, BA.2, and BA.5

Author

10759509, Tamura, Tomokazu, Yamasoba, Daichi, Oda, Yoshitaka, Ito, Jumpei, Kamasaki, Tomoko, Nao, Naganori, Hashimoto, Rina, Fujioka, Yoichiro, Suzuki, Rigel, Wang, Lei, Ito, Hayato, Kashima, Yukie, Kimura, Izumi, Kishimoto, Mai, Tsuda, Masumi, Sawa, Hirofumi, Yoshimatsu, Kumiko, Yamamoto, Yuki, Nagamoto, Tetsuharu, Kanamune, Jun, Suzuki, Yutaka, Ohba, Yusuke, The Genotype to Phenotype Japan (G2P-Japan) Consortium, Yokota, Isao, Matsuno, Keita, Takayama, Kazuo, Tanaka, Shinya, Sato, Kei, Fukuhara, Takasuke, 10759509, Tamura, Tomokazu, Yamasoba, Daichi, Oda, Yoshitaka, Ito, Jumpei, Kamasaki, Tomoko, Nao, Naganori, Hashimoto, Rina, Fujioka, Yoichiro, Suzuki, Rigel, Wang, Lei, Ito, Hayato, Kashima, Yukie, Kimura, Izumi, Kishimoto, Mai, Tsuda, Masumi, Sawa, Hirofumi, Yoshimatsu, Kumiko, Yamamoto, Yuki, Nagamoto, Tetsuharu, Kanamune, Jun, Suzuki, Yutaka, Ohba, Yusuke, The Genotype to Phenotype Japan (G2P-Japan) Consortium, Yokota, Isao, Matsuno, Keita, Takayama, Kazuo, Tanaka, Shinya, Sato, Kei, and Fukuhara, Takasuke

Abstract

The unremitting emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants necessitates ongoing control measures. Given its rapid spread, the new Omicron subvariant BA.5 requires urgent characterization. Here, we comprehensively analyzed BA.5 with the other Omicron variants BA.1, BA.2, and ancestral B.1.1. Although in vitro growth kinetics of BA.5 was comparable among the Omicron subvariants, BA.5 was much more fusogenic than BA.1 and BA.2. Airway-on-a-chip analysis showed that, among Omicron subvariants, BA.5 had enhanced ability to disrupt the respiratory epithelial and endothelial barriers. Furthermore, in our hamster model, in vivo pathogenicity of BA.5 was slightly higher than that of the other Omicron variants and less than that of ancestral B.1.1. Notably, BA.5 gains efficient virus spread compared with BA.1 and BA.2, leading to prompt immune responses. Our findings suggest that BA.5 has low pathogenicity compared with the ancestral strain but enhanced virus spread /inflammation compared with earlier Omicron subvariants.

Published
2023

28. Schlafen family member 11 indicates favorable prognosis of patients with head and neck cancer following platinum-based chemoradiotherapy

Author

Hamada, Seijiro, Kano, Satoshi, Murai, Junko, Suzuki, Takayoshi, Tsushima, Nayuta, Mizumachi, Takatsugu, Suzuki, Masanobu, Takashima, Tsuyoshi, Taniyama, Daiki, Sakamoto, Naoya, Fujioka, Yoichiro, Ohba, Yusuke, Homma, Akihiro, Hamada, Seijiro, Kano, Satoshi, Murai, Junko, Suzuki, Takayoshi, Tsushima, Nayuta, Mizumachi, Takatsugu, Suzuki, Masanobu, Takashima, Tsuyoshi, Taniyama, Daiki, Sakamoto, Naoya, Fujioka, Yoichiro, Ohba, Yusuke, and Homma, Akihiro

Abstract

Recently, Schlafen family member 11 (SLFN11) has been reported to increase the sensitivity of cancer cells to DNA-damaging agents, including platinum derivatives; thus, SLFN11 may be a predictive biomarker for platinum-based chemoradiotherapy (CRT). In this study, we examined whether SLFN11 expression was associated with the therapeutic outcome of platinum-based CRT in head and neck squamous cell carcinoma (HNSCC). We performed immunohistochemical analyses for SLFN11 expression in 161 HNSCC tissues from patients who had been administered cisplatin-based CRT and examined the correlation between SLFN11 expression and progression-free survival (PFS). Additionally, SLFN11 expression was examined in 10 paired samples obtained before and after CRT in patients with local failure. Furthermore, in vitro experiments were performed using several HNSCC cell lines and isogenic SLFN11-knockout cells to assess the association between SLFN11 expression and drug sensitivity. PFS was found to be significantly better in the SLFN11-positive group than in the SLFN11-negative group among the 161 patients (5-year PFS: 78.8% vs. 52.8%, respectively, p < 0.001). Similar results were observed for the PFS at each primary site. The percentage of SLFN11 positivity was lower in tumor samples from patients with local failure after CRT than that in the corresponding primary tumors before CRT in 8 of 10 cases. Results of the in vitro assay demonstrated that SLFN11-knockout cells exhibited reduced sensitivity to DNA-damaging agents but not to the non-DNA-damaging agent docetaxel. Our findings suggest that SLFN11 may serve as a potential biomarker for predicting the response of HNSCC patients to platinum-based CRT.

Published
2023

39. Schlafen family member 11 indicates favorable prognosis of patients with head and neck cancer following platinum-based chemoradiotherapy

Author

Hamada, Seijiro, primary, Kano, Satoshi, additional, Murai, Junko, additional, Suzuki, Takayoshi, additional, Tsushima, Nayuta, additional, Mizumachi, Takatsugu, additional, Suzuki, Masanobu, additional, Takashima, Tsuyoshi, additional, Taniyama, Daiki, additional, Sakamoto, Naoya, additional, Fujioka, Yoichiro, additional, Ohba, Yusuke, additional, and Homma, Akihiro, additional

Published
2023
Full Text
View/download PDF

45. Lypd8 promotes the segregation of flagellated microbiota and colonic epithelia

Author

Okumura, Ryu, Kurakawa, Takashi, Nakano, Takashi, Kayama, Hisako, Kinoshita, Makoto, Motooka, Daisuke, Gotoh, Kazuyoshi, Kimura, Taishi, Kamiyama, Naganori, Kusu, Takashi, Ueda, Yoshiyasu, Wu, Hong, Iijima, Hideki, Barman, Soumik, Osawa, Hideki, Matsuno, Hiroshi, Nishimura, Junichi, Ohba, Yusuke, Nakamura, Shota, Iida, Tetsuya, Yamamoto, Masahiro, Umemoto, Eiji, Sano, Koichi, and Takeda, Kiyoshi

Subjects
Microbiota (Symbiotic organisms) -- Physiological aspects, Colon (Anatomy) -- Physiological aspects, Epithelial cells -- Physiological aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Colonic epithelial cells are covered by thick inner and outer mucus layers (1,2). The inner mucus layer is free of commensal microbiota, which contributes to the maintenance of gut homeostasis (3-6). In the small intestine, molecules critical for prevention of bacterial invasion into epithelia such as Paneth-cell-derived anti-microbial peptides and regenerating islet-derived 3 (RegIII) family proteins have been identified (7-11). Although there are mucus layers providing physical barriers against the large number of microbiota present in the large intestine, the mechanisms that separate bacteria and colonic epithelia are not fully elucidated. Here we show that Ly6/ PLAUR domain containing 8 (Lypd8) protein prevents flagellated microbiota invading the colonic epithelia in mice. Lypd8, selectively expressed in epithelial cells at the uppermost layer of the large intestinal gland, was secreted into the lumen and bound flagellated bacteria including Proteus mirabilis. In the absence of Lypd8, bacteria were present in the inner mucus layer and many flagellated bacteria invaded epithelia. [Lypd8.sup.-/-] mice were highly sensitive to intestinal inflammation induced by dextran sulfate sodium (DSS). Antibiotic elimination of Gram-negative flagellated bacteria restored the bacterial-free state of the inner mucus layer and ameliorated DSS-induced intestinal inflammation in [Lypd8.sup.-/-] mice. Lypd8 bound to flagella and suppressed motility of flagellated bacteria. Thus, Lypd8 mediates segregation of intestinal bacteria and epithelial cells in the colon to preserve intestinal homeostasis., The intestinal mucosa is protected from commensal microbes and pathogenic microorganisms by several types of barriers (5,6), including mucus layers produced by intestinal secretory cells (1). Compared with those in [...]

Published
2016

46. Beneficial innate signaling interference for antibacterial responses by a Toll-like receptor–mediated enhancement of the MKP-IRF3 axis

Author

Negishi, Hideo, Matsuki, Kosuke, Endo, Nobuyasu, Sarashina, Hana, Miki, Shoji, Matsuda, Atsushi, Fukazawa, Keiko, Taguchi-Atarashi, Naoko, Ikushima, Hiroaki, Yanai, Hideyuki, Nishio, Junko, Honda, Kenya, Fujioka, Yoichiro, Ohba, Yusuke, Noda, Tetsuo, Taniguchi, Shun'ichiro, Nishida, Eisuke, Zhang, Yongliang, Chi, Hongbo, Flavell, Richard A., and Taniguchi, Tadatsugu

Published
2013

47. Direct visualization of glucagon-like peptide-1 secretion by fluorescent fusion proteins

Author

Tsuzuki, Atsushi, 1000070597492, Fujioka, Yoichiro, 1000070831288, Yoshida, Aiko, 1000060880002, Kashiwagi, Sayaka, 1000080365808, Amano, Maho, 1000010396301, Hira, Tohru, 1000070552420, Nakamura, Akinobu, 1000030360902, Miyoshi, Hideaki, 1000020301905, Atsumi, Tatsuya, 1000030333503, Ohba, Yusuke, Tsuzuki, Atsushi, 1000070597492, Fujioka, Yoichiro, 1000070831288, Yoshida, Aiko, 1000060880002, Kashiwagi, Sayaka, 1000080365808, Amano, Maho, 1000010396301, Hira, Tohru, 1000070552420, Nakamura, Akinobu, 1000030360902, Miyoshi, Hideaki, 1000020301905, Atsumi, Tatsuya, 1000030333503, and Ohba, Yusuke

Abstract

Live-cell imaging with fluorescent proteins (FPs) is a powerful tool for investigating the exocytosis processes of hormones. However, the secretion process of glucagon-like peptide-1 (GLP-1) has not been visualized by FPs, which might be because tagging FPs inhibits GLP-1 synthesis through the post-translational processing from proglucagon. Here, we have developed FP-tagged GLP-1 by inserting FPs into the middle of GLP-1 and adding the proglucagon signal peptide. Confocal imaging confirmed that GLP-1 fused to FPs with high folding efficiency showed granular structure, in which secretory vesicle markers colocalized. The fluorescence intensity of FP in the culture supernatant from cells treated with KCl or forskolin was significantly increased compared with those from untreated cells. Furthermore, FP-tagged GLP-1 enables direct visualization of stimulation-dependent exocytosis of GLP-1 at a single granule resolution with total internal reflection fluorescence microscopy. FP-tagged GLP-1 might facilitate the screening of GLP-1 secretagogues and the discovery of new antidiabetic drugs.

Published
2022

48. Secretory glycoprotein NS1 plays a crucial role in the particle formation of flaviviruses

Author

Tamura, Tomokazu, Torii, Shiho, Kajiwara, Kentaro, Anzai, Itsuki, 1000070597492, Fujioka, Yoichiro, Noda, Kisho, Taguwa, Shuhei, Morioka, Yuhei, Suzuki, Rigel, Fauzyah, Yuzy, Ono, Chikako, 1000030333503, Ohba, Yusuke, Okada, Masato, 1000070598739, Fukuhara, Takasuke, Matsuura, Yoshiharu, Tamura, Tomokazu, Torii, Shiho, Kajiwara, Kentaro, Anzai, Itsuki, 1000070597492, Fujioka, Yoichiro, Noda, Kisho, Taguwa, Shuhei, Morioka, Yuhei, Suzuki, Rigel, Fauzyah, Yuzy, Ono, Chikako, 1000030333503, Ohba, Yusuke, Okada, Masato, 1000070598739, Fukuhara, Takasuke, and Matsuura, Yoshiharu

Abstract

Flaviviruses, which are globally distributed and cause a spectrum of potentially severe illnesses, pose a major threat to public health. Although Flaviviridae viruses, including flaviviruses, possess similar genome structures, only the flaviviruses encode the non-structural protein NS1, which resides in the endoplasmic reticulum (ER) and is secreted from cells after oligomerization. The ER-resident NS1 is known to be involved in viral genome replication, but the essential roles of secretory NS1 in the virus life cycle are not fully understood. Here we characterized the roles of secretory NS1 in the particle formation of flaviviruses. We first identified an amino acid residue essential for the NS1 secretion but not for viral genome replication by using protein-protein interaction network analyses and mutagenesis scanning. By using the recombinant flaviviruses carrying the identified NS1 mutation, we clarified that the mutant flaviviruses employed viral genome replication. We then constructed a recombinant NS1 with the identified mutation and demonstrated by physicochemical assays that the mutant NS1 was unable to form a proper oligomer or associate with liposomes. Finally, we showed that the functions of NS1 that were lost by the identified mutation could be compensated for by the in trans-expression of E-rns of pestiviruses and host exchangeable apolipoproteins, which participate in the infectious particle formation of pestiviruses and hepaciviruses in the family Flaviviridae, respectively. Collectively, our study suggests that secretory NS1 plays a role in the particle formation of flaviviruses through its interaction with the lipid membrane. Author summaryIt is difficult to characterize the function of NS1 in the post-genome replication stages in the virus life cycle of flaviviruses. Here, by means of protein-protein interaction network analyses and mutagenesis scanning, we identified a unique mutation in NS1 by which the protein loses its secretory capacity while

Published
2022

Catalog

Books, media, physical & digital resources
See catalog results