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Your search keyword '"Ohmori, Shinnosuke"' showing total 11 results
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11 results on '"Ohmori, Shinnosuke"'

2. The cleistogamy of the superwoman1-cleistogamy1 mutation is sensitive to low temperatures during the lodicule-forming stage

Author

Ohmori, Shinnosuke, Koike, Setsuo, Hayashi, Takami, Yamaguchi, Tomoya, Kuroki, Makoto, and Yoshida, Hitoshi

Subjects
lodicule, thermosensitive, outcrossing, flower opening, cleistogamy, food and beverages, genetically modified rice, gene flow, Research Paper
Abstract

We reported previously that the rice (Oryza sativa L.) cleistogamous mutation superwoman1-cleistogamy1 (spw1-cls1) was applicable to inhibit outcrossing between genetically modified varieties and their relatives, which causes pollen-mediated gene flow or disturbance of line purity. The cleistogamy of spw1-cls1 is caused by decreased protein-protein interactions between the mutant SPW1 and its partner proteins. Importantly, these interactions are restored under low-temperature conditions, but whether the cleistogamy of spw1-cls1 is affected by this phenomenon was unclear. In this study, we cultivated spw1-cls1 in various regions of Japan and confirmed that its flowers opened at low temperatures. Moreover, we compared the morphology of a series of lodicules generated at various temperatures. The results indicated that the cleistogamy of spw1-cls1 is thermosensitive and is gradually disturbed as the temperature decreases. This was correlated with the protein interaction pattern of the mutant SPW1 as reported previously. Then, we revealed the critical period for the low-temperature-induced instability of the phenotype of spw1-cls1 and examined the effect of daily temperature changes on cleistogamy. The results may facilitate simulation of the phenotype of spw1-cls1 at various temperatures and the prediction of regions where the cleistogamy of spw1-cls1 can be stably used to inhibit outcrossing.

Published
2018

4. Thesuperwoman1-cleistogamy2mutant is a novel resource for gene containment in rice

Author

Lombardo, Fabien, primary, Kuroki, Makoto, additional, Yao, Shan-Guo, additional, Shimizu, Hiroyuki, additional, Ikegaya, Tomohito, additional, Kimizu, Mayumi, additional, Ohmori, Shinnosuke, additional, Akiyama, Takashi, additional, Hayashi, Takami, additional, Yamaguchi, Tomoya, additional, Koike, Setsuo, additional, Yatou, Osamu, additional, and Yoshida, Hitoshi, additional

Published
2016
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6. The superwoman1-cleistogamy2 mutant is a novel resource for gene containment in rice.

Author

Lombardo, Fabien, Kuroki, Makoto, Yao, Shan‐Guo, Shimizu, Hiroyuki, Ikegaya, Tomohito, Kimizu, Mayumi, Ohmori, Shinnosuke, Akiyama, Takashi, Hayashi, Takami, Yamaguchi, Tomoya, Koike, Setsuo, Yatou, Osamu, and Yoshida, Hitoshi

Subjects
RICE genetics, CLEISTOGAMY, TRANSCRIPTION factors, PHENOTYPES, DNA-binding proteins
Abstract

Outcrossing between cultivated plants and their related wild species may result in the loss of favourable agricultural traits in the progeny or escape of transgenes in the environment. Outcrossing can be physically prevented by using cleistogamous (i.e. closed-flower) plants. In rice, flower opening is dependent on the mechanical action of fleshy organs called lodicules, which are generally regarded as the grass petal equivalents. Lodicule identity and development are specified by the action of protein complexes involving the SPW1 and Os MADS2 transcription factors. In the superwoman1-cleistogamy1 ( spw1-cls1) mutant, SPW1 is impaired for heterodimerization with Os MADS2 and consequently spw1-cls1 shows thin, ineffective lodicules. However, low temperatures help stabilise the mutated SPW1/Os MADS2 heterodimer and lodicule development is restored when spw1-cls1 is grown in a cold environment, resulting in the loss of the cleistogamous phenotype. To identify a novel, temperature-stable cleistogamous allele of SPW1, targeted and random mutations were introduced into the SPW1 sequence and their effects over SPW1/Os MADS2 dimer formation were assessed in yeast two-hybrid experiments. In parallel, a novel cleistogamous allele of SPW1 called spw1-cls2 was isolated from a forward genetic screen. In spw1-cls2, a mutation leading to a change of an amino acid involved in DNA binding by the transcription factor was identified. Fertility of spw1-cls2 is somewhat decreased under low temperatures but unlike for spw1-cls1, the cleistogamous phenotype is maintained, making the line a safer and valuable genetic resource for gene containment. [ABSTRACT FROM AUTHOR]

Published
2017
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7. Cleistogamy in crops—molecular mechanism and utilization in breeding

Author

Yoshida, Hitoshi, primary, Ohmori, Shinnosuke, additional, Yatou, Osamu, additional, Komatsuda, Takao, additional, Wang, Ning, additional, Ebitani, Takeshi, additional, Fujita, Kenji, additional, Yamaguchi, Takuya, additional, Kitani, Yoshinori, additional, Fujita, Masaya, additional, and Kubo, Katashi, additional

Published
2014
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