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204 results on '"Oishi, Isao"'

17. Identification of a member of the MAPKKK family as a potential mediator of TGF-beta signal transduction

Author

Yamaguchi, Kyoko, Shirakabe, Kyoko, Shibuya, Hiroshi, Irie, Kenji, Oishi, Isao, Ueno, Naoto, Taniguchi, Tadatsugu, Nishida, Eisuke, and Matsumoto, Kunihiro

Subjects
Protein kinases -- Research, Transforming growth factors -- Research, Science and technology, Research
Abstract

The mitogen-activated protein kinase (MAPK) pathway is a conserved eukaryotic signaling module that converts receptor signals into various outputs. MAPK is activated through phosphorylation by MAPK kinase (MAPKK), which is first activated by MAPKK kinase (MAPKKK). A genetic selection based on a MAPK pathway in yeast was used to identify a mouse protein kinase (TAK1) distinct from other members of the MAPKKK family. TAK1 was shown to participate in regulation of transcription by transforming growth factor-o (TGF-β). Furthermore, kinase activity of TAK1 was stimulated in response to TGF-P and bone morphogenetic protein. These results suggest that TAK1 functions as a mediator in the signaling pathway of TGF-β superfamily members., Activation of MAPKs after ligand binding to various receptors has been correlated with numerous cellular responses, including proliferation, differentiation, and regulation of specific metabolic pathways in differentiated cell types. The [...]

Published
1995

18. An IRF-1-dependent pathway of DNA damage-induced apoptosis in mitogen-activated T lymphocytes

Author

Tamura, Tomohiko, Ishihara, Masahiko, Lamphier, Marc S., Tanaka, Nobuyuki, Oishi, Isao, Aizawa, Shinichi, Matsuyama, Toshifumi, Mak, Tak W., Taki, Shinsuke, and Taniguchi, Tadatsugu

Subjects
T cells -- Physiological aspects, Cell death -- Research, Tumor suppressor genes -- Research, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Interferon regulatory factor (IRF)-1 appears to be a tumor suppressor whose inactivation may be linked with myelodysplastic syndrome and leukemia development. There may be two different DNA damage-induced apoptosis pathways for T lymphocytes. IRF-1 appears to function in mitogen-stimulated mature T lymphocytes while the previously accepted p53 pathway functions in thymocytes.

Published
1995

21. Cre-LoxP-regulated expression of monoclonal antibodies driven by an ovalbumin promoter in primary oviduct cells

Author

Esteban Concepcion, Yoshii Kyoko, Kim Sungtae, Oishi Isao, and Izpisua Belmonte Juan

Subjects
Biotechnology, TP248.13-248.65
Abstract

Abstract Background A promoter capable of driving high-level transgene expression in oviduct cells is important for developing transgenic chickens capable of producing therapeutic proteins, including monoclonal antibodies (mAbs), in the whites of laid eggs. Ovalbumin promoters can be used as oviduct-specific regulatory sequences in transgenic chickens, but their promoter activities are not high, according to previous reports. Results In this study, while using a previously characterized ovalbumin promoter, we attempted to improve the expression level of mAbs using a Cre/loxP-mediated conditional excision system. We constructed a therapeutic mAb expression vector, pBS-DS-hIgG, driven by the CMV and CAG promoters, in which the expression of the heavy and light chains of humanized immunoglobulin G (hIgG) is preceded by two floxed stuffer reporter genes. In the presence of Cre, the stuffer genes were precisely excised and hIgG expression was induced in pBS-DS-hIgG-transfected 293T cells. In chicken oviduct primary culture cells, hIgG was expressed after transfection of pBS-DS-hIgG together with the ovalbumin promoter-driven Cre expression vector. The expression level of hIgG in these cells was increased 40-fold over that induced directly by the ovalbumin promoter. On the other hand, hIgG was not induced by the ovalbumin promoter-driven Cre in chicken embryonic fibroblast cells. Conclusions The Cre/loxP-based system could significantly increase ovalbumin promoter-driven production of proteins of interest, specifically in oviduct cells. This expression system could be useful for producing therapeutic mAbs at high level using transgenic chickens as bioreactors.

Published
2011
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23. Characterization of Norwalk virus GI specific monoclonal antibodies generated against Escherichia coli expressed capsid protein and the reactivity of two broadly reactive monoclonal antibodies generated against GII capsid towards GI recombinant fragments

Author

Takino Koichi, Utagawa Etsuko, Kawamoto Hiroyoshi, Kuzuguchi Tsuyoshi, Oishi Isao, Yamazaki Kenji, Suzuki Yasuhiko, Terano Yoshitake, Yoda Tomoko, Oda Hajime, and Shibata Tadayoshi

Subjects
Microbiology, QR1-502
Abstract

Abstract Background Norwalk virus causes outbreaks of acute non-bacterial gastroenteritis in humans. The virus capsid is composed of a single 60 kDa protein. In a previous study, the capsid protein of recombinant Norwalk virus genogroup II was expressed in an E. coli system and monoclonal antibodies were generated against it. The analysis of the reactivity of those monoclonal antibodies suggested that the N-terminal domain might contain more antigenic epitopes than the C-terminal domain. In the same study, two broadly reactive monoclonal antibodies were observed to react with genogroup I recombinant protein. Results In the present study, we used the recombinant capsid protein of genogroup I and characterized the obtained 17 monoclonal antibodies by using 19 overlapping fragments. Sixteen monoclonal antibodies recognized sequential epitopes on three antigenic regions, and the only exceptional monoclonal antibody recognized a conformational epitope. As for the two broadly reactive monoclonal antibodies generated against genogroup II, we indicated that they recognized fragment 2 of genogroup I. Furthermore, genogroup I antigen from a patient's stool was detected by sandwich enzyme-linked immunosorbent assay using genogroup I specific monoclonal antibody and biotinated broadly reactive monoclonal antibody. Conclusion The reactivity analysis of above monoclonal antibodies suggests that the N-terminal domain may contain more antigenic epitopes than the C-terminal domain as suggested in our previous study. The detection of genogroup I antigen from a patient's stool by our system suggested that the monoclonal antibodies generated against E. coli expressed capsid protein can be used to detect genogroup I antigens in clinical material.

Published
2001
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28. Culture Conditions for Maintain Propagation, Long-term Survival and Germline Transmission of Chicken Primordial Germ Cell-Like Cells

Author

Miyahara, Daichi, Mori, Takafumi, Makino, Ryuichi, Nakamura, Yoshiaki, Oishi, Isao, Ono, Tamao; WFfCPpkh, Nirasawa, Keijiro, Tagami, Takahiro, Kagami, Hiroshi; OeyUPUkh, Miyahara, Daichi, Mori, Takafumi, Makino, Ryuichi, Nakamura, Yoshiaki, Oishi, Isao, Ono, Tamao; WFfCPpkh, Nirasawa, Keijiro, Tagami, Takahiro, and Kagami, Hiroshi; OeyUPUkh

Abstract

Transplantation of primordial germ cells (PGCs), which are the progenitor cells of gametes, is a powerful tool for generation of transgenic chickens. However, the frequencies of transgene integration into the genome of purified PGCs still remain low. An in vitro culture system enabling chicken PGCs to propagate efficiently would be useful for efficient transgenesis of PGCs. In the present study, we optimized the culture conditions for chicken PGCs to enhance the proliferation and evaluated the germline transmission of cultured PGCs that proliferated for long periods of time. PGC-like cells (PGC-LCs), that have remarkably similar morphological characteristics to intact PGCs, could be derived by cultivation of blood containing PGCs obtained from 2.5-day-old chicken embryos according to the protocol of van de Lavoir et al. (2006). We determined which feeder cells and which growth factors were required to improve proliferation of PGC-LCs. Male PGC-LCs survival and proliferation were enhanced during culture in the basic medium containing either basic fibroblast growth factor (bFGF) alone or both bFGF and stem cell factor (SCF) on a feeder of buffalo rat liver (BRL) cells. Male PGC-LCs could be propagated in defined culture condition for extended periods. These cells expressed the germline-specific protein Vasa and undifferentiated cell marker stage-specific embryonic antigen-1 (SSEA-1) and pluripotency genes Nanog and PouV. Furthermore, Male PGC-LCs cultured for 225 d could migrate toward and colonize within recipient gonads and transmit to the next generation following transplantation. We succeeded in produce 3 offspring originating from long-term cultured PGC-LCs from a germline chimeric rooster (6%). The present study represents valuable steps toward defining a culture condition enabling PGC-LCs to propagate efficiently for long periods in vitro with maintenance of their commitment to the germline.

Published
2014

39. HIV Type 1 Subtypes in Circulation in Northern Kenya

Author

Khamadi, Samoel A., primary, Ochieng, Washington, additional, Lihana, Raphael W., additional, Kinyua, Joyceline, additional, Muriuki, Joseph, additional, Mwangi, Joseph, additional, Lwembe, Raphael, additional, Kiptoo, Michael, additional, Osman, Saida, additional, Lagat, Nancy, additional, Pelle, Roger, additional, Muigai, Anne, additional, Carter, Jane Y., additional, Oishi, Isao, additional, Ichimura, Hiroshi, additional, Mwaniki, D.L., additional, Okoth, Fredrick A., additional, Mpoke, Solomon, additional, and Songok, Elijah M., additional

Published
2005
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46. Loss of mRor1 Enhances the Heart and Skeletal Abnormalities in mRor2-Deficient Mice: Redundant and Pleiotropic Functions of mRor1 and mRor2 Receptor Tyrosine Kinases

Author

Nomi, Masashi, primary, Oishi, Isao, additional, Kani, Shuichi, additional, Suzuki, Hiroaki, additional, Matsuda, Takeru, additional, Yoda, Akinori, additional, Kitamura, Makiko, additional, Itoh, Kyoko, additional, Takeuchi, Shigeto, additional, Takeda, Kiyoshi, additional, Akira, Shizuo, additional, Ikeya, Makoto, additional, Takada, Shinji, additional, and Minami, Yasuhiro, additional

Published
2001
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