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26 results on '"Oki, Choji"'

2. Video 3 Intravital imaging of BrafV600E melanoma cells expressing GCaMP6s [parental, Gnaq-/-, and Gnaq-/- cells introduced with Gnaq (Gnaq-/- + Gnaq)]. from Intravital Imaging Identifies the VEGF–TXA2 Axis as a Critical Promoter of PGE2 Secretion from Tumor Cells and Immune Evasion

Author

Konishi, Yoshinobu, primary, Ichise, Hiroshi, primary, Watabe, Tetsuya, primary, Oki, Choji, primary, Tsukiji, Shinya, primary, Hamazaki, Yoko, primary, Murakawa, Yasuhiro, primary, Takaori-Kondo, Akifumi, primary, Terai, Kenta, primary, and Matsuda, Michiyuki, primary

Published
2023
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3. Video 4 Intravital imaging of BrafV600E melanoma cells expressing GCaMP6s with or without treatment of a VEGFR inhibitor, motesanib. from Intravital Imaging Identifies the VEGF–TXA2 Axis as a Critical Promoter of PGE2 Secretion from Tumor Cells and Immune Evasion

Author

Konishi, Yoshinobu, primary, Ichise, Hiroshi, primary, Watabe, Tetsuya, primary, Oki, Choji, primary, Tsukiji, Shinya, primary, Hamazaki, Yoko, primary, Murakawa, Yasuhiro, primary, Takaori-Kondo, Akifumi, primary, Terai, Kenta, primary, and Matsuda, Michiyuki, primary

Published
2023
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4. Video 2 Live imaging of BrafV600E melanoma cells expressing GCaMP6s in vivo and in vitro. from Intravital Imaging Identifies the VEGF–TXA2 Axis as a Critical Promoter of PGE2 Secretion from Tumor Cells and Immune Evasion

Author

Konishi, Yoshinobu, primary, Ichise, Hiroshi, primary, Watabe, Tetsuya, primary, Oki, Choji, primary, Tsukiji, Shinya, primary, Hamazaki, Yoko, primary, Murakawa, Yasuhiro, primary, Takaori-Kondo, Akifumi, primary, Terai, Kenta, primary, and Matsuda, Michiyuki, primary

Published
2023
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5. Video 1 Live imaging of MDCK cells expressing the PKA biosensor. from Intravital Imaging Identifies the VEGF–TXA2 Axis as a Critical Promoter of PGE2 Secretion from Tumor Cells and Immune Evasion

Author

Konishi, Yoshinobu, primary, Ichise, Hiroshi, primary, Watabe, Tetsuya, primary, Oki, Choji, primary, Tsukiji, Shinya, primary, Hamazaki, Yoko, primary, Murakawa, Yasuhiro, primary, Takaori-Kondo, Akifumi, primary, Terai, Kenta, primary, and Matsuda, Michiyuki, primary

Published
2023
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8. Intravital imaging identifies the VEGF-TXA₂ axis as a critical promoter of PGE₂ secretion from tumor cells and immune evasion

Author

Konishi, Yoshinobu, Ichise, Hiroshi, Watabe, Tetsuya, Oki, Choji, Tsukiji, Shinya, Hamazaki, Yoko, Murakawa, Yasuhiro, Takaori-Kondo, Akifumi, Terai, Kenta, and Matsuda, Michiyuki

Abstract

Prostaglandin E₂ (PGE₂) promotes tumor progression through evasion of anti-tumor immunity. In stark contrast to cyclooxygenase-dependent production of PGE₂, little is known whether or not PGE₂ secretion is regulated within tumor tissues. Here, we show that VEGF-dependent release of thromboxane A₂ (TXA₂) triggers Ca²⁺ transients in tumor cells, culminating in PGE₂ secretion and subsequent immune evasion in the early stages of tumorigenesis. Ca²⁺ transients caused cPLA2 activation and triggered the arachidonic acid cascade. Ca²⁺ transients were monitored as the surrogate marker of PGE₂ secretion. Intravital imaging of BrafV600E mouse melanoma cells revealed that the proportion of cells exhibiting Ca²⁺ transients is markedly higher in vivo than in vitro. The TXA₂ receptor was indispensable for the Ca²⁺ transients in vivo, high intra-tumoral PGE₂ concentration, and evasion of anti-tumor immunity. Notably, treatment with a vascular endothelial growth factor (VEGF) receptor antagonist and an anti-VEGF antibody rapidly suppressed Ca²⁺ transients and reduced TXA₂ and PGE₂ concentrations in tumor tissues. These results identify the VEGF-TXA₂ axis as a critical promoter of PGE₂-dependent tumor immune evasion, providing a molecular basis underlying the immunomodulatory effect of anti-VEGF therapies., がん細胞が免疫から逃れるメカニズムの解明 --がん細胞と血管内皮細胞との細胞間相互作用--. 京都大学プレスリリース. 2021-05-28.

Published
2021

10. Functional visualization of NK Cell-mediated killing of metastatic single tumor cells

Author

20616073, 10199812, Ichise, Hiroshi, Tsukamoto, Shoko, Hirashima, Tsuyoshi, Konishi, Yoshinobu, Oki, Choji, Tsukiji, Shinya, Iwano, Satoshi, Miyawaki, Atsushi, Sumiyama, Kenta, Terai, Kenta, Matsuda, Michiyuki, 20616073, 10199812, Ichise, Hiroshi, Tsukamoto, Shoko, Hirashima, Tsuyoshi, Konishi, Yoshinobu, Oki, Choji, Tsukiji, Shinya, Iwano, Satoshi, Miyawaki, Atsushi, Sumiyama, Kenta, Terai, Kenta, and Matsuda, Michiyuki

Abstract

Natural killer (NK) cells lyse invading tumor cells to limit metastatic growth in the lung, but how some cancers evade this host protective mechanism to establish a growing lesion is unknown. Here we have combined ultra-sensitive bioluminescence imaging with intravital two-photon microscopy involving genetically-encoded biosensors to examine this question. NK cells eliminated disseminated tumor cells from the lung within 24 hrs of arrival, but not thereafter. Intravital dynamic imaging revealed that 50% of NK-tumor cell encounters lead to tumor cell death in the first 4 hrs after tumor cell arrival, but after 24 hrs of arrival, nearly 100% of the interactions result in the survival of the tumor cell. During this 24 hrs period, the probability of ERK activation in NK cells upon encountering the tumor cells was decreased from 68% to 8%, which correlated with the loss of the activating ligand CD155/PVR/Necl5 from the tumor cell surface. Thus, by quantitatively visualizing the NK-tumor cell interaction at the early stage of metastasis, we have revealed the crucial parameters of NK cell immune surveillance in the lung.

Published
2022

14. Intravital imaging identifies the VEGF-TXA₂ axis as a critical promoter of PGE₂ secretion from tumor cells and immune evasion

Author

10362477, 50765469, 20616073, 10199812, Konishi, Yoshinobu, Ichise, Hiroshi, Watabe, Tetsuya, Oki, Choji, Tsukiji, Shinya, Hamazaki, Yoko, Murakawa, Yasuhiro, Takaori-Kondo, Akifumi, Terai, Kenta, Matsuda, Michiyuki, 10362477, 50765469, 20616073, 10199812, Konishi, Yoshinobu, Ichise, Hiroshi, Watabe, Tetsuya, Oki, Choji, Tsukiji, Shinya, Hamazaki, Yoko, Murakawa, Yasuhiro, Takaori-Kondo, Akifumi, Terai, Kenta, and Matsuda, Michiyuki

Abstract

Prostaglandin E₂ (PGE₂) promotes tumor progression through evasion of anti-tumor immunity. In stark contrast to cyclooxygenase-dependent production of PGE₂, little is known whether or not PGE₂ secretion is regulated within tumor tissues. Here, we show that VEGF-dependent release of thromboxane A₂ (TXA₂) triggers Ca²⁺ transients in tumor cells, culminating in PGE₂ secretion and subsequent immune evasion in the early stages of tumorigenesis. Ca²⁺ transients caused cPLA2 activation and triggered the arachidonic acid cascade. Ca²⁺ transients were monitored as the surrogate marker of PGE₂ secretion. Intravital imaging of BrafV600E mouse melanoma cells revealed that the proportion of cells exhibiting Ca²⁺ transients is markedly higher in vivo than in vitro. The TXA₂ receptor was indispensable for the Ca²⁺ transients in vivo, high intra-tumoral PGE₂ concentration, and evasion of anti-tumor immunity. Notably, treatment with a vascular endothelial growth factor (VEGF) receptor antagonist and an anti-VEGF antibody rapidly suppressed Ca²⁺ transients and reduced TXA₂ and PGE₂ concentrations in tumor tissues. These results identify the VEGF-TXA₂ axis as a critical promoter of PGE₂-dependent tumor immune evasion, providing a molecular basis underlying the immunomodulatory effect of anti-VEGF therapies.

Published
2021

15. Author Reply to Peer Reviews of Metastatic Single Tumor Cells Evade NK Cell-mediated Killing by Thrombin-mediated Loss of the Activating Ligand CD155/PVR/Necl-5

Author

Matsuda, Michiyuki, primary, Terai, Kenta, additional, Sumiyama, Kenta, additional, Miyawaki, Atsushi, additional, Iwano, Satoshi, additional, Tsukiji, Shinya, additional, Oki, Choji, additional, Konishi, Yoshinobu, additional, Hirashima, Tsuyoshi, additional, Tsukamoto, Shoko, additional, and Ichise, Hiroshi, additional

Published
2021
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16. Metastatic Single Tumor Cells Evade NK Cell-mediated Killing by Thrombin-mediated Loss of the Activating Ligand CD155/PVR/Necl-5

Author

Ichise, Hiroshi, primary, Tsukamoto, Shoko, additional, Hirashima, Tsuyoshi, additional, Konishi, Yoshinobu, additional, Oki, Choji, additional, Tsukiji, Shinya, additional, Iwano, Satoshi, additional, Miyawaki, Atsushi, additional, Sumiyama, Kenta, additional, Terai, Kenta, additional, and Matsuda, Michiyuki, additional

Published
2021
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17. Abstract PO-057: Host cell-derived TXA2-mediated Gq signaling in tumor cells promotes tumor immune evasion

Author

Terai, Kenta, primary, Konishi, Yoshinobu, additional, Ichise, Hiroshi, additional, Watabe, Tetsuya, additional, Sando, Yukari, additional, Kondo, Takefumi, additional, Oki, Choji, additional, Tsukiji, Shinya, additional, Hamazaki, Yoko, additional, Murakawa, Yasuhiro, additional, Takaori-Kondo, Akifumi, additional, and Matsuda, Michiyuki, additional

Published
2020
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25. Intravital Imaging Identifies the VEGF-TXA 2 Axis as a Critical Promoter of PGE 2 Secretion from Tumor Cells and Immune Evasion.

Author

Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, Murakawa Y, Takaori-Kondo A, Terai K, and Matsuda M

Subjects
Animals, Humans, Mice, Mice, Nude, Dinoprostone immunology, Immune Evasion immunology, Intravital Microscopy methods, Vascular Endothelial Growth Factor A immunology
Abstract

Prostaglandin E 2 (PGE 2 ) promotes tumor progression through evasion of antitumor immunity. In stark contrast to cyclooxygenase-dependent production of PGE 2 , little is known whether PGE 2 secretion is regulated within tumor tissues. Here, we show that VEGF-dependent release of thromboxane A 2 (TXA 2 ) triggers Ca 2+ transients in tumor cells, culminating in PGE 2 secretion and subsequent immune evasion in the early stages of tumorigenesis. Ca 2+ transients caused cPLA2 activation and triggered the arachidonic acid cascade. Ca 2+ transients were monitored as the surrogate marker of PGE 2 secretion. Intravital imaging of Braf V600E mouse melanoma cells revealed that the proportion of cells exhibiting Ca 2+ transients is markedly higher in vivo than in vitro . The TXA 2 receptor was indispensable for the Ca 2+ transients in vivo , high intratumoral PGE 2 concentration, and evasion of antitumor immunity. Notably, treatment with a VEGF receptor antagonist and an anti-VEGF antibody rapidly suppressed Ca 2+ transients and reduced TXA 2 and PGE 2 concentrations in tumor tissues. These results identify the VEGF-TXA 2 axis as a critical promoter of PGE 2 -dependent tumor immune evasion, providing a molecular basis underlying the immunomodulatory effect of anti-VEGF therapies. SIGNIFICANCE: This study identifies the VEGF-TXA 2 axis as a potentially targetable regulator of PGE 2 secretion, which provides novel strategies for prevention and treatment of multiple types of malignancies., (©2021 American Association for Cancer Research.)

Published
2021
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26. Stabilization of Charge Carriers in Picket-Fence Polythiophenes Using Dielectric Side Chains.

Author

Zhao C, Sakurai T, Yoneda S, Seki S, Sugimoto M, Oki C, Takeuchi M, and Sugiyasu K

Abstract

Insulated molecular wires (IMWs) are π-conjugated polymers that are molecularly sheathed with an insulating layer and are structurally analogous to electric power cords at the nanoscale. Such unique architectures are expected in molecular electronics and organic devices. Herein, we propose a new molecular design concept of IMWs, in which the sheaths can be customized, thereby enabling the modulation of the electronic properties of the interior π-conjugated systems. To this end, we focused our attention on the dielectric constant of the sheaths, as it governs the electrostatic interaction between charges. Upon doping, charge carriers, such as polaron and bipolaron, were generated regardless of the dielectric properties of the sheaths. Flash-photolysis time-resolved microwave conductivity measurements revealed that intrawire charge carrier mobility was independent of the sheaths. However, we found that the charge carriers could be stabilized by the sheaths with a high dielectric constant owing to the charge screening effect. We expect that IMWs designed in this way will be useful in a variety of applications, where the nature of charge carriers plays an important role, and particularly when redox switching is required (e.g., electrochromic, magnetic, and memory applications)., (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2016
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