160 results on '"Olewicz-Gawlik A"'
Search Results
2. Self-reactive IgE and anti-IgE therapy in autoimmune diseases
- Author
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Anna Olewicz-Gawlik and Arleta Kowala-Piaskowska
- Subjects
Immunoglobulin E ,anti-IgE ,omalizumab ,autoimmunity ,autoimmune disease ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Growing evidence indicates the pathogenic role of autoreactive IgE in autoimmune diseases. Incidence of autoimmune and allergic diseases in the industrialized countries is consistently icreasing, thus leading to concerted efforts to comprehend the regulation of IgE-mediated mechanisms. The first reports of a presence of IgE autoantibodies in patients with autoimmune diseases have been published a long time ago, and it is now recognized that self-reactive IgE can mediate inflammatory response in bullous pemhigoid, systemic lupus erythematosus, chronic urticaria, and atopic dermatitis. The advances in understanding the pathomechanisms of these disorders brought to a successful use of anti-IgE strategies in their management. The present review discusses the current state of knowledge on the IgE-mediated autoimmunity and anti-IgE treatment, and pave the way for further exploration of the subject.
- Published
- 2023
- Full Text
- View/download PDF
3. Autoimmune hemolytic anemia: current knowledge and perspectives
- Author
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Sylwia Sulimiera Michalak, Anna Olewicz-Gawlik, Joanna Rupa-Matysek, Edyta Wolny-Rokicka, Elżbieta Nowakowska, and Lidia Gil
- Subjects
Autoimmune hemolytic anemia ,Cold agglutinin disease ,Pathogenesis ,Microvesicles ,Shear stress ,Treatment ,Immunologic diseases. Allergy ,RC581-607 ,Geriatrics ,RC952-954.6 - Abstract
Abstract Autoimmune hemolytic anemia (AIHA) is an acquired, heterogeneous group of diseases which includes warm AIHA, cold agglutinin disease (CAD), mixed AIHA, paroxysmal cold hemoglobinuria and atypical AIHA. Currently CAD is defined as a chronic, clonal lymphoproliferative disorder, while the presence of cold agglutinins underlying other diseases is known as cold agglutinin syndrome. AIHA is mediated by autoantibodies directed against red blood cells (RBCs) causing premature erythrocyte destruction. The pathogenesis of AIHA is complex and still not fully understood. Recent studies indicate the involvement of T and B cell dysregulation, reduced CD4+ and CD25+ Tregs, increased clonal expansions of CD8 + T cells, imbalance of Th17/Tregs and Tfh/Tfr, and impaired lymphocyte apoptosis. Changes in some RBC membrane structures, under the influence of mechanical stimuli or oxidative stress, may promote autohemolysis. The clinical presentation and treatment of AIHA are influenced by many factors, including the type of AIHA, degree of hemolysis, underlying diseases, presence of concomitant comorbidities, bone marrow compensatory abilities and the presence of fibrosis and dyserthropoiesis. The main treatment for AIHA is based on the inhibition of autoantibody production by mono- or combination therapy using GKS and/or rituximab and, rarely, immunosuppressive drugs or immunomodulators. Reduction of erythrocyte destruction via splenectomy is currently the third line of treatment for warm AIHA. Supportive treatment including vitamin supplementation, recombinant erythropoietin, thrombosis prophylaxis and the prevention and treatment of infections is essential. New groups of drugs that inhibit immune responses at various levels are being developed intensively, including inhibition of antibody-mediated RBCs phagocytosis, inhibition of B cell and plasma cell frequency and activity, inhibition of IgG recycling, immunomodulation of T lymphocytes function, and complement cascade inhibition. Recent studies have brought about changes in classification and progress in understanding the pathogenesis and treatment of AIHA, although there are still many issues to be resolved, particularly concerning the impact of age-associated changes to immunity.
- Published
- 2020
- Full Text
- View/download PDF
4. Evaluation of elafin as a marker of skin fibrosis – a preliminary study.
- Author
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Polanska, Adriana, primary, Kowalczyk, Michał J., additional, Olewicz-Gawlik, Anna, additional, Łojko-Dankowska, Anna, additional, Walecka, Irena, additional, Ciechanowicz, Piotr, additional, Żaba, Ryszard, additional, and Dańczak-Pazdrowska, Aleksandra, additional
- Published
- 2023
- Full Text
- View/download PDF
5. Autoimmune hemolytic anemia: current knowledge and perspectives
- Author
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Michalak, Sylwia Sulimiera, Olewicz-Gawlik, Anna, Rupa-Matysek, Joanna, Wolny-Rokicka, Edyta, Nowakowska, Elżbieta, and Gil, Lidia
- Published
- 2020
- Full Text
- View/download PDF
6. Serum alarm antiproteases in systemic sclerosis patients
- Author
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Olewicz-Gawlik, Anna, Trzybulska, Dorota, Graniczna, Katarzyna, Kuznar-Kaminska, Barbara, Katulska, Katarzyna, Batura-Gabryel, Halina, Frydrychowicz, Magdalena, Danczak-Pazdrowska, Aleksandra, and Mozer-Lisewska, Iwona
- Published
- 2017
- Full Text
- View/download PDF
7. Patterns of glomerular disease based on 4-year kidney biopsy material analyzed by light microscopy and immunofluorescence: a retrospective single-center analysis in Poland
- Author
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Anna Olewicz-Gawlik, Dorota Sikorska, Agnieszka Pluto-Prądzyńska, Magdalena Roszak, Ilona Idasiak-Piechocka, Krzysztof Pawlaczyk, Andrzej Oko, Aldona Woźniak, Katarzyna Iwanik, Grzegorz Dworacki, Jan Sikora, and Jan Żeromski
- Subjects
renal biopsy ,glomerulonephritis ,epidemiology ,Medicine - Abstract
The aim of this study was to assess the epidemiology of different patterns of chronic glomerular diseases based on clinical, histopathological and immunofluorescent findings of glomerulonephritis patients hospitalized in the Department of Nephrology, Transplantology and Internal Diseases in Poznan between January 2009 and December 2012. We retrospectively studied 418 patients who had been subjected to renal biopsies. Data on serum creatinine concentration, 24 h proteinuria, arterial hypertension, diabetes mellitus, and histological and immunofluorescent findings were collected. The patients’ mean age was 42 ±15. The male sex prevailed (53.1%). Immunoglobulin A nephropathy was the most common finding (18.9%), followed by focal segmental glomerulosclerosis (16.3%), membranous glomerulonephritis (10.1%), lupus nephritis (8.4%), extracapillary glomerulonephritis (3.3%) and membranoproliferative glomerulonephritis (2.6%). In 69 (16.5%) patients the biopsy was non-informative or non-diagnostic. Patients with membranous nephropathy presented the highest frequency of nephrotic syndrome (71.4%), followed by membranoproliferative glomerulonephritis and focal segmental glomerulosclerosis. Combined analysis of the clinical, histopathological and immunofluorescent findings in glomerulonephritis patients based on a single center’s data can provide important epidemiological findings.
- Published
- 2017
- Full Text
- View/download PDF
8. Serum Clara cell 16-kDa protein levels and lung impairment in systemic sclerosis patients
- Author
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Olewicz-Gawlik, Anna, Trzybulska, Dorota, Kuznar-Kaminska, Barbara, Katulska, Katarzyna, Danczak-Pazdrowska, Aleksandra, Batura-Gabryel, Halina, and Hrycaj, Pawel
- Published
- 2016
- Full Text
- View/download PDF
9. Níveis séricos de proteína de células de Clara de 16 kDa e comprometimento pulmonar em pacientes com esclerose sistêmica
- Author
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Olewicz‐Gawlik, Anna, Trzybulska, Dorota, Kuznar‐Kaminska, Barbara, Katulska, Katarzyna, Danczak‐Pazdrowska, Aleksandra, Batura‐Gabryel, Halina, and Hrycaj, Pawel
- Published
- 2016
- Full Text
- View/download PDF
10. Self-reactive IgE and anti-IgE therapy in autoimmune diseases
- Author
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Olewicz-Gawlik, Anna, primary and Kowala-Piaskowska, Arleta, additional
- Published
- 2023
- Full Text
- View/download PDF
11. Changes in Inflammatory Markers after Administration of Tocilizumab in COVID-19: A Single-Center Retrospective Study
- Author
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Anna Olewicz-Gawlik, Barbara Ginter-Matuszewska, Mikołaj Kamiński, Agnieszka Adamek, Maciej Bura, Iwona Mozer-Lisewska, and Arleta Kowala-Piaskowska
- Subjects
musculoskeletal diseases ,tocilizumab ,SARS-CoV-2 ,antiviral ,Poland ,COVID-19 ,interleukin-6 ,Medicine ,General Medicine ,skin and connective tissue diseases ,Article - Abstract
The COVID-19 pandemic requires the development of effective methods for the treatment of severe cases. We aimed to describe clinical outcomes and changes in inflammatory markers in Polish patients treated with tocilizumab. The medical charts of SARS-CoV-2-positive patients treated in the Department of Infectious Diseases between 4 March and 2 September 2020 were retrospectively analyzed. The patients who received tocilizumab according to the Polish Association of Epidemiologists and Infectiologists guidelines were selected for the study. We identified 29 individuals who received tocilizumab, out of whom 11 (37.9%) died. The individuals who died had significantly higher maximal interleukin-6 (IL-6) and lactate dehydrogenase (LDH) serum levels than survivors. After administration of tocilizumab, further increase in LDH and IL-6 was a prognostic factor for unfavorable outcomes. Among inflammatory markers, 7-day mean of IL-6 serum concentration was the best predictor of death (cut-off: ≥417 pg/mL; area under ROC curve = 0.81 [95% Confidence Interval: 0.63–0.98]). The serum concentrations of inflammatory markers before administration of tocilizumab did not predict the outcome, whereas IL-6 and LDH measurements after administration of tocilizumab seemed to be of predictive value.
- Published
- 2022
12. The importance of residual renal function in peritoneal dialysis
- Author
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Sikorska, Dorota, Pawlaczyk, Krzysztof, Olewicz-Gawlik, Anna, Czepulis, Natasza, Posnik, Bartlomiej, Baum, Ewa, Wanic-Kossowska, Maria, Lindholm, Bengt, and Oko, Andrzej
- Published
- 2016
- Full Text
- View/download PDF
13. Factors associated with overhydration in peritoneal dialysis patients
- Author
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Dorota Sikorska, Krzysztof Pawlaczyk, Anna Olewicz-Gawlik, Magdalena Roszak, Włodzimierz Samborski, Ewa Baum, Małgorzata Kałużna, Maria Wanic-Kossowska, Bengt Lindholm, and Andrzej Oko
- Subjects
peritoneal dialysis ,bioelectrical impedance analysis ,state of hydration ,Medicine - Abstract
Aim. Overhydration is a prevalent problem in peritoneal dialysis (PD) patients. The aim of the study was to evaluate the effect of several factors on the development of overhydration in PD patients. Material and Methods. The study was performed on 74 PD patients, who were divided into two groups according to bioimpedance analysis hydration status (OHBIA): Group A OHBIA
- Published
- 2017
- Full Text
- View/download PDF
14. Autoimmune hemolytic anemia: current knowledge and perspectives
- Author
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Elżbieta Nowakowska, Edyta Wolny-Rokicka, Joanna Rupa-Matysek, Anna Olewicz-Gawlik, Sylwia Sulimiera Michalak, and Lidia Gil
- Subjects
0301 basic medicine ,lcsh:Immunologic diseases. Allergy ,Aging ,Cold agglutinin disease ,Immunology ,Review ,Pathogenesis ,lcsh:Geriatrics ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Medicine ,Paroxysmal cold hemoglobinuria ,Autoimmune hemolytic anemia ,B cell ,Shear stress ,business.industry ,Autoantibody ,medicine.disease ,Cold Agglutinin ,Treatment ,lcsh:RC952-954.6 ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Rituximab ,business ,lcsh:RC581-607 ,Microvesicles ,medicine.drug - Abstract
Autoimmune hemolytic anemia (AIHA) is an acquired, heterogeneous group of diseases which includes warm AIHA, cold agglutinin disease (CAD), mixed AIHA, paroxysmal cold hemoglobinuria and atypical AIHA. Currently CAD is defined as a chronic, clonal lymphoproliferative disorder, while the presence of cold agglutinins underlying other diseases is known as cold agglutinin syndrome. AIHA is mediated by autoantibodies directed against red blood cells (RBCs) causing premature erythrocyte destruction. The pathogenesis of AIHA is complex and still not fully understood. Recent studies indicate the involvement of T and B cell dysregulation, reduced CD4+ and CD25+ Tregs, increased clonal expansions of CD8 + T cells, imbalance of Th17/Tregs and Tfh/Tfr, and impaired lymphocyte apoptosis. Changes in some RBC membrane structures, under the influence of mechanical stimuli or oxidative stress, may promote autohemolysis. The clinical presentation and treatment of AIHA are influenced by many factors, including the type of AIHA, degree of hemolysis, underlying diseases, presence of concomitant comorbidities, bone marrow compensatory abilities and the presence of fibrosis and dyserthropoiesis. The main treatment for AIHA is based on the inhibition of autoantibody production by mono- or combination therapy using GKS and/or rituximab and, rarely, immunosuppressive drugs or immunomodulators. Reduction of erythrocyte destruction via splenectomy is currently the third line of treatment for warm AIHA. Supportive treatment including vitamin supplementation, recombinant erythropoietin, thrombosis prophylaxis and the prevention and treatment of infections is essential. New groups of drugs that inhibit immune responses at various levels are being developed intensively, including inhibition of antibody-mediated RBCs phagocytosis, inhibition of B cell and plasma cell frequency and activity, inhibition of IgG recycling, immunomodulation of T lymphocytes function, and complement cascade inhibition. Recent studies have brought about changes in classification and progress in understanding the pathogenesis and treatment of AIHA, although there are still many issues to be resolved, particularly concerning the impact of age-associated changes to immunity.
- Published
- 2020
15. Choroby zakaźne i pasożytnicze
- Author
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Anna Boroń-Kaczmarska, Alicja Wiercińska-Drapało, Brygida Adamek, Monika Bociąga-Jasik, Aneta Cybula, Zenon Czuba, Justyna Dunaj-Małyszko, Katarzyna Dzierżanowska-Fangrat, Ewa Firląg-Burkacka, Maria Gańczak, Aleksander Garlicki, Mariusz Gąsior, Justyna Glik, Małgorzata Inglot, Joanna Jabłońska, Ewa Janczewska, Jerzy Jaroszewicz, Marek Kawecki, Lucjan Kępa, Brygida Knysz, Maciej Kondrusik, Katarzyna Kosińska-Kaczyńska, Arleta Kowala-Piaskowska, Joanna Kozłowska, Adam Krajewski, Dagny Krankowska, Anna Kuna, Włodzimierz Mazur, Tomasz Mikuła, Anna Moniuszko-Malinowska, Iwona Mozer-Lisewska, Alicja Nowowiejska-Wiewióra, Barbara Oczko-Grzesik, Anita Olczak, Anna Olewicz-Gawlik, Maria Olszyńska-Krowicka, Sławomir Pancewicz, Miłosz Parczewski, Bronisława Pietrzak, Damian Piotrowski, Mariusz Sapuła, Ewa Siwak, Barbara Sobala-Szczygieł, Wojciech Stańczak, Wojciech Stolarz, Magdalena Suchacz, Leszek Szenborn, Bartosz Szetela, Mirosław Wielgoś, and Andrzej Załęski
- Published
- 2022
16. Choroby zakaźne i pasożytnicze
- Author
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Boroń-Kaczmarska, Anna, primary, Wiercińska-Drapało, Alicja, additional, Adamek, Brygida, additional, Bociąga-Jasik, Monika, additional, Cybula, Aneta, additional, Czuba, Zenon, additional, Dunaj-Małyszko, Justyna, additional, Dzierżanowska-Fangrat, Katarzyna, additional, Firląg-Burkacka, Ewa, additional, Gańczak, Maria, additional, Garlicki, Aleksander, additional, Gąsior, Mariusz, additional, Glik, Justyna, additional, Inglot, Małgorzata, additional, Jabłońska, Joanna, additional, Janczewska, Ewa, additional, Jaroszewicz, Jerzy, additional, Kawecki, Marek, additional, Kępa, Lucjan, additional, Knysz, Brygida, additional, Kondrusik, Maciej, additional, Kosińska-Kaczyńska, Katarzyna, additional, Kowala-Piaskowska, Arleta, additional, Kozłowska, Joanna, additional, Krajewski, Adam, additional, Krankowska, Dagny, additional, Kuna, Anna, additional, Mazur, Włodzimierz, additional, Mikuła, Tomasz, additional, Moniuszko-Malinowska, Anna, additional, Mozer-Lisewska, Iwona, additional, Nowowiejska-Wiewióra, Alicja, additional, Oczko-Grzesik, Barbara, additional, Olczak, Anita, additional, Olewicz-Gawlik, Anna, additional, Olszyńska-Krowicka, Maria, additional, Pancewicz, Sławomir, additional, Parczewski, Miłosz, additional, Pietrzak, Bronisława, additional, Piotrowski, Damian, additional, Sapuła, Mariusz, additional, Siwak, Ewa, additional, Sobala-Szczygieł, Barbara, additional, Stańczak, Wojciech, additional, Stolarz, Wojciech, additional, Suchacz, Magdalena, additional, Szenborn, Leszek, additional, Szetela, Bartosz, additional, Wielgoś, Mirosław, additional, and Załęski, Andrzej, additional
- Published
- 2022
- Full Text
- View/download PDF
17. Changes in Inflammatory Markers after Administration of Tocilizumab in COVID-19: A Single-Center Retrospective Study
- Author
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Olewicz-Gawlik, Anna, primary, Ginter-Matuszewska, Barbara, additional, Kamiński, Mikołaj, additional, Adamek, Agnieszka, additional, Bura, Maciej, additional, Mozer-Lisewska, Iwona, additional, and Kowala-Piaskowska, Arleta, additional
- Published
- 2021
- Full Text
- View/download PDF
18. Soluble selectins and highly fucosylated ?1-antichymotrypsin in rheumatoid arthritis patients
- Author
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Anna Olewicz-Gawlik, Izabela Korczowska-Łącka, and Paweł Hrycaj
- Subjects
acute phase proteins ,fucosylation ,rheumatoid arthritis ,selectins ,Medicine - Abstract
Introduction. Fucosylation of acute phase proteins and serum soluble selectin levels is increased in rheumatoid arthritis (RA) patients and can influence leukocyte extravasation. Aim. The aim of this study was to evaluate the concentration and fucosylation of ?1-antichymotrypsin (ACT) in relation to serum concentrations of soluble forms of selectins in RA patients. Material and methods. Serum samples of 70 RA patients and 30 healthy controls were examined using sandwich enzyme-linked immunosorbent assay (ELISA). Results. ACT-FR was significantly increased in RA patients when compared to healthy controls (p
- Published
- 2015
- Full Text
- View/download PDF
19. Blood serum levels of amino-terminal pro-C-type natriuretic peptide in patients with rheumatoid arthritis
- Author
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Olewicz-Gawlik, A, Trzybulska, D, Grala, P, and Hrycaj, P
- Published
- 2010
- Full Text
- View/download PDF
20. A case of bizarre posttransplant anti-glomerular basement membrane disease
- Author
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Anna Olewicz-Gawlik, Dorota Sikorska, Ilona Idasiak-Piechocka, Monika Świerczewska, Andrzej Oko, Husam Samara, Jan Sikora, Magdalena Pawlik, Grzegorz Dworacki, and Jan Żeromski
- Subjects
Pathology ,medicine.medical_specialty ,Immunology ,kidney transplantation ,lcsh:Medicine ,Case Report ,Mesangial hypercellularity ,Immunofluorescence ,Anti-Glomerular Basement Membrane Disease ,urologic and male genital diseases ,Type IV collagen ,medicine ,Immunology and Allergy ,immunofluorescence ,Kidney transplantation ,Basement membrane ,Kidney ,anti-glomerular basement membrane disease ,medicine.diagnostic_test ,business.industry ,urogenital system ,lcsh:R ,Glomerulonephritis ,medicine.disease ,medicine.anatomical_structure ,business ,glomerulonephritis - Abstract
The non-collagenous (NC1) domain of α3 and α5 chains of type IV collagen are eminent targets of abnormal immune response in anti-glomerular basement membrane (anti-GBM) disease, which can be diagnosed by the presence of strong linear IgG staining along GBM detected by direct immunofluorescence. The presence of linear GBM fixation in renal allograft is a rare finding. We observed a 33-year-old male with de novo renal failure in a kidney transplant. An examination of a kidney biopsy specimen revealed, in light microscopy, mild mesangial hypercellularity together with mild focal interstitial fibrosis and sparse inflammatory infiltrate. In immunofluorescence microscopy strong linear IgG staining along the capillary walls was seen. Serum anti-GBM antibodies were negative and no mutation in exons coding NC1 domains of α3 and α5 chains of type IV collagen were detected. We described a rare case of a patient with atypical anti-GBM disease in renal allograft, caused probably by the same process which affected the native kidneys.
- Published
- 2019
21. Interleukin-17 and interleukin-23: importance in the pathogenesis of lung impairment in patients with systemic sclerosis
- Author
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OLEWICZ-GAWLIK, Anna, DANCZAK-PAZDROWSKA, Aleksandra, KUZNAR-KAMINSKA, Barbara, GORNOWICZ-POROWSKA, Justyna, KATULSKA, Katarzyna, TRZYBULSKA, Dorota, BATURA-GABRYEL, Halina, SILNY, Wojciech, POPLAWSKI, Dariusz, and HRYCAJ, Pawel
- Published
- 2014
- Full Text
- View/download PDF
22. Large bone distractor for open reconstruction of articular fractures of the calcaneus
- Author
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Grala, Pawel, Twardosz, Wojciech, Tondel, Wieslaw, Olewicz-Gawlik, Anna, and Hrycaj, Pawel
- Published
- 2009
- Full Text
- View/download PDF
23. Does low-dose and short-term glucocorticoids treatment increase the risk of osteoporosis in rheumatoid arthritis female patients?
- Author
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Korczowska, Izabela, Olewicz-Gawlik, Anna, Trefler, Jakub, Hrycaj, Paweł, and Krzysztof Łącki, Jan
- Published
- 2008
- Full Text
- View/download PDF
24. Fucosylation of serum α1-acid glycoprotein in rheumatoid arthritis patients treated with infliximab
- Author
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Olewicz-Gawlik, Anna, Korczowska-Łącka, Izabela, Łącki, Jan K., Klama, Kamilla, and Hrycaj, Paweł
- Published
- 2007
- Full Text
- View/download PDF
25. The importance of hypoalbuminemia in peritoneal dialysis patients: Impact of gender
- Author
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Dorota Sikorska, Anna Olewicz-Gawlik, Andrzej Oko, Krzysztof Pawlaczyk, and Ewa Baum
- Subjects
Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,030232 urology & nephrology ,Serum albumin ,Medicine (miscellaneous) ,Gastroenterology ,General Biochemistry, Genetics and Molecular Biology ,Peritoneal dialysis ,03 medical and health sciences ,0302 clinical medicine ,Renal Dialysis ,Internal medicine ,Electric Impedance ,Internal Medicine ,medicine ,Humans ,Pharmacology (medical) ,Hypoalbuminemia ,Serum Albumin ,Genetics (clinical) ,Dialysis ,Aged ,030222 orthopedics ,biology ,Troponin T ,business.industry ,Middle Aged ,Brain natriuretic peptide ,medicine.disease ,C-Reactive Protein ,Reviews and References (medical) ,biology.protein ,Kidney Failure, Chronic ,Female ,business ,Peritoneal Dialysis ,Body mass index ,Bioelectrical impedance analysis - Abstract
Background High mortality in peritoneal dialysis (PD) patients is associated with the presence of nontraditional cardiovascular risk factors, such as malnutrition. However, hypoalbuminemia in patients undergoing PD may have gender-dependent consequences. Objectives The aim of the study was to evaluate the relationship between hypoalbuminemia, overhydration (OH), inflammation, and cardiovascular risk, depending on gender. Material and methods The group studied consisted of 54 PD patients: 26 male (mean age: 59 ±19 years) and 28 female (mean age: 52 ±15 years). Serum albumin levels were measured routinely by the hospital central laboratory. The degree of OH was assessed by bioelectrical impedance analysis (BIA). Serum concentrations of C-reactive protein (CRP) and interleukin (IL)-6 were measured as inflammatory markers. Levels of N-terminal prohormone of brain natriuretic peptide (NT-proBNP) and troponin T (TnT) were used to assess cardiovascular risk. Results Median serum albumin concentration was 3.9 g/dL (3.6-4.2 g/dL). Both genders were comparable regarding most parameters except body weight (79 ±16 kg vs 67 ±16 kg; p = 0.009), but no differences were observed in body mass index (BMI) (26.3 ±5.0 kg/m2 vs 26.2 ±5.9 kg/m2; non significant (NS)). There was also no difference in the prevalence of hypoalbuminemia between female and male PD patients (23% vs 21%; NS). In females, low serum albumin concentrations were associated with OH, inflammation and cardiovascular risk, while in males serum albumin levels correlated with the parameters of dialysis and cardiovascular risk. Conclusions The impact of hypoalbuminemia may be gender-dependent. It seems that hypoalbuminemia is more important for female patients. It is also possible that different mechanisms regulate serum albumin concentration in female and male PD patients.
- Published
- 2018
26. Levels of EGF and VEGF in patients with primary and secondary Sjögren’s syndrome
- Author
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Katarzyna Błochowiak, Jerzy Sokalski, Michalina Nowak-Gabryel, Jan Sikora, Dorota Trzybulska, Jarosław Kocięcki, Anna Olewicz-Gawlik, and Henryk Witmanowski
- Subjects
Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,Saliva ,Arginine ,Angiogenesis ,Medicine (miscellaneous) ,Salivary Glands, Minor ,Polymerase Chain Reaction ,Peripheral blood mononuclear cell ,General Biochemistry, Genetics and Molecular Biology ,Pathogenesis ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Epidermal growth factor ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Pharmacology (medical) ,Genetics (clinical) ,030203 arthritis & rheumatology ,Epidermal Growth Factor ,business.industry ,Healthy Volunteers ,Vascular endothelial growth factor ,stomatognathic diseases ,Sjogren's Syndrome ,Real-time polymerase chain reaction ,Endocrinology ,chemistry ,Reviews and References (medical) ,Leukocytes, Mononuclear ,Female ,business ,Biomarkers ,030215 immunology - Abstract
Background Aberrant angiogenesis plays a role in the pathogenesis of Sjogren's syndrome (SS). Objectives The aim of this study was to compare the levels of vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) in stimulatory parotid saliva and in serum in healthy subjects (HS), patients with primary SS (pSS) and secondary SS (sSS) and to evaluate the expression of EGF, proangiogenic VEGF165 and antiangiogenic VEGF165 b mRNA isoforms. Additionally, we determined the salivary levels of serine/arginine splicing factor (SRSF1), which regulates VEGF165 and VEGF165 b expression. Material and methods The study comprised 34 women (16 with pSS and 18 with sSS) and healthy subjects for blood and saliva sampling. EGF and VEGF levels in saliva and serum and salivary SRSF1 levels were determined by enzyme-linked immunosorbent assay (ELISA). The expression of VEGF165 , VEGF165 b and EGF in peripheral blood mononuclear cells (PBMC) was evaluated by quantitative polymerase chain reaction (qPCR). Results There were no differences in the levels of EGF, VEGF, SRSF1 and in the expression of the EGF, VEGF165 and VEGF165 b between HS and SS patients, or between pSS and sSS patients. The salivary levels of VEGF165 and EGF were significantly higher in pSS, sSS and HS than serum levels. Levels of SRSF1 correlated positively with VEGF and EGF levels. Levels of EGF, VEGF and SRSF1 correlated with each other. Conclusions The balance of VEGF isoforms is not disturbed in SS. Saliva is more sensitive for the detection of EGF and VEGF than serum, but salivary levels of those proteins are not representative for SS.
- Published
- 2018
27. Serum alarm antiproteases in systemic sclerosis patients
- Author
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Magdalena Frydrychowicz, Katarzyna Katulska, Dorota Trzybulska, Iwona Mozer-Lisewska, Barbara Kuznar-Kaminska, Halina Batura-Gabryel, Katarzyna Graniczna, Anna Olewicz-Gawlik, and Aleksandra Dańczak-Pazdrowska
- Subjects
Adult ,Male ,0301 basic medicine ,Immunology ,Arthritis, Rheumatoid ,Pathogenesis ,Young Adult ,03 medical and health sciences ,DLCO ,Diffusing capacity ,medicine ,Humans ,Immunology and Allergy ,Protease Inhibitors ,Secretory Leukocyte Peptidase Inhibitor ,skin and connective tissue diseases ,Aged ,Aged, 80 and over ,Scleroderma, Systemic ,Lung ,business.industry ,General Medicine ,Middle Aged ,Acquired immune system ,medicine.disease ,Immunity, Innate ,Elafin ,030104 developmental biology ,medicine.anatomical_structure ,Rheumatoid arthritis ,Female ,business ,Biomarkers ,SLPI - Abstract
Alarm antiproteases, i.e. secretory leukocyte protease inhibitor ad elafin, are key mediators in innate immune response and integrate innate and adaptive immunity systems. The aim of the study was to assess clinical significance of serum levels of alarm antiproteases, elafin and secretory leukocyte protease inhibitor (SLPI) in patients with systemic sclerosis (SSc). Twenty-eight patients with SSc, 25 patients with rheumatoid arthritis (RA) and 22 healthy controls were recruited. Serum elafin and SLPI levels were examined using enzyme-linked immunosorbent assay (ELISA). The patients with SSc had significantly increased serum levels of SLPI in comparison with the RA patients and the healthy controls (p
- Published
- 2017
28. Serum ICAM-1, VCAM-1 and E-selectin levels in patients with primary and secondary Sjögren’s syndrome
- Author
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Anna Olewicz-Gawlik, Henryk Witmanowski, Dorota Trzybulska, Jarosław Kocięcki, Katarzyna Błochowiak, Jerzy Sokalski, and Michalina Nowak-Gabryel
- Subjects
0301 basic medicine ,Medicine (miscellaneous) ,Pilot Projects ,Gastroenterology ,chemistry.chemical_compound ,0302 clinical medicine ,Pharmacology (medical) ,Xerophthalmia ,Genetics (clinical) ,ICAM-1 ,medicine.diagnostic_test ,biology ,Middle Aged ,Intercellular Adhesion Molecule-1 ,Sjogren's Syndrome ,Erythrocyte sedimentation rate ,Female ,E-Selectin ,Adult ,medicine.medical_specialty ,Adolescent ,Visual analogue scale ,Vascular Cell Adhesion Molecule-1 ,Enzyme-Linked Immunosorbent Assay ,Blood Sedimentation ,Xerostomia ,General Biochemistry, Genetics and Molecular Biology ,Young Adult ,03 medical and health sciences ,stomatognathic system ,Internal medicine ,E-selectin ,Internal Medicine ,medicine ,Humans ,VCAM-1 ,Aged ,030203 arthritis & rheumatology ,business.industry ,Case-control study ,medicine.disease ,eye diseases ,SSS ,stomatognathic diseases ,030104 developmental biology ,chemistry ,Case-Control Studies ,Reviews and References (medical) ,biology.protein ,business ,Biomarkers - Abstract
Background Typical features of Sjogren's syndrome (SS) are severe xerostomia and xerophthalmia which are basic diagnostic criteria. Objectives The aim of this study was to compare the serum levels of soluble (s) intercellular adhesion molecule 1 (sICAM-1), vascular cell adhesion molecule 1 (sVCAM-1) and sE-selectin between primary (pSS), secondary (sSS) and healthy subjects (HS). We correlated these results with selected clinical parameters of disease activity and parameters of the severity of xerostomia and xerophthalmia. Material and methods The serum levels of sICAM-1, sVCAM-1 and sE-selectin were determined by enzyme-linked immunosorbent assay (ELISA) in 16 patients with pSS, 18 with sSS and 15 HS. Eye dryness and xerostomia were assessed by the Schirmer's test, the Fox test and the visual analogue scale (VAS). Results The levels of sICAM-1 in pSS and sVCAM-1 in sSS patients were significantly higher when compared to HS (p = 0.02 and p = 0.048, respectively). There were no differences between pSS and sSS. In pSS, sVCAM-1 correlated positively with VAS (rS = 0.52, p = 0.04) and the Fox test (rS = 0.66, p=0.01). In sSS, sE-selectin correlated positively with sICAM-1 (rS = 0.54, p = 0.01), the duration of the disease (rS = 0.51, p = 0.03) and negatively with the Schirmer's test (rS = 0.59, p = 0.04). sICAM-1 correlated positively with the erythrocyte sedimentation rate (ESR) value (rS = 0.59, p = 0.01). Conclusions sVCAM-1 reflects xerostomia in pSS. sICAM-1 and sE-selectin may be additional parameters of sSS activity.
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- 2017
29. The importance of residual renal function in peritoneal dialysis
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Andrzej Oko, Bengt Lindholm, Maria Wanic-Kossowska, Natasza Czepulis, Anna Olewicz-Gawlik, Ewa Baum, Dorota Sikorska, Krzysztof Pawlaczyk, and Bartlomiej Posnik
- Subjects
Adult ,Male ,medicine.medical_specialty ,Urology ,medicine.medical_treatment ,Statistics as Topic ,Water-Electrolyte Imbalance ,030232 urology & nephrology ,Peripheral edema ,Diuresis ,Renal function ,030204 cardiovascular system & hematology ,Kidney Function Tests ,Lower risk ,Gastroenterology ,Peritoneal dialysis ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Edema ,Natriuretic Peptide, Brain ,medicine ,Humans ,Ejection fraction ,Troponin T ,business.industry ,Middle Aged ,Peptide Fragments ,Renal Elimination ,Endocrinology ,Cardiovascular Diseases ,Echocardiography ,Nephrology ,Kidney Failure, Chronic ,Female ,Poland ,medicine.symptom ,business ,Peritoneal Dialysis ,Biomarkers - Abstract
Peritoneal dialysis (PD) patients with preserved residual diuresis have a lower risk of death and complications. Here we analyzed associations between residual diuresis and presence of fluid overload and biomarkers of cardiac strain and nutrition in PD patients. Among 44 PD patients placed into three subgroups, depending on volume of residual diuresis (group A ≤ 500; group B 600–1900; and group C ≥ 2000 mL/day), we examined: overhydration (OH) assessed by bioimpedance analysis (BIA; yielding OH index OHBIA) and by clinical criteria (edema and hypertension); nutritional status (by subjective global assessment, SGA); metabolic status (electrolytes, serum lipid profile, CRP, and albumin); biomarkers of fluid overload and cardiac strain (N-terminal probrain natriuretic peptide, NT-proBNP, and troponin T, TnT); and, echocardiography and chest X-ray. With increasing residual diuresis in group A, B and C, fewer patients had signs of overhydration defined as OHBIA > 1.1 L (75.0, 42.9 and 33.3 %) or peripheral edema (25.0, 21.4 and 0 %) and NT-proBNP (15199 ± 16150 vs. 5930 ± 9256 vs. 2600 ± 3907 pg/mL; p
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- 2016
30. Serum Clara cell 16-kDa protein levels and lung impairment in systemic sclerosis patients
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Dorota Trzybulska, Halina Batura-Gabryel, Paweł Hrycaj, Katarzyna Katulska, Anna Olewicz-Gawlik, Aleksandra Dańczak-Pazdrowska, and Barbara Kuznar-Kaminska
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Pathology ,medicine.medical_specialty ,High-resolution computed tomography ,Doença intersticial pulmonar ,lcsh:Diseases of the musculoskeletal system ,Enzyme-Linked Immunosorbent Assay ,Interstitial lung disease ,Disease ,03 medical and health sciences ,0302 clinical medicine ,Diffusing capacity ,Esclerose sistêmica ,medicine ,Humans ,Plethysmograph ,Lung volumes ,Lung ,Plethysmography, Whole Body ,General Environmental Science ,030203 arthritis & rheumatology ,Scleroderma, Systemic ,medicine.diagnostic_test ,business.industry ,Blood Proteins ,respiratory system ,medicine.disease ,respiratory tract diseases ,medicine.anatomical_structure ,030228 respiratory system ,Case-Control Studies ,Clara cell ,Systemic sclerosis ,General Earth and Planetary Sciences ,lcsh:RC925-935 ,Lung Diseases, Interstitial ,business ,Biomarkers ,CC16 - Abstract
Objective: To assess clinical utility of serum Clara cell 16-kDa protein measurements in relation with staging system for systemic sclerosis associated interstitial lung disease. Materials and methods: Serum levels of Clara cell 16-kDa protein were determined by ELISA in 28 systemic sclerosis patients and 30 healthy controls, and correlated with staging system for systemic sclerosis associated interstitial lung disease in systemic sclerosis patients. Lung involvement was assessed functionally (body plethysmography, diffusing capacity of the lung for carbon monoxide) and radiologically (an average disease extent on high resolution computed tomography of the lungs) in SSc patients. Results: We observed statistically significant differences in serum Clara cell 16-kDa protein levels between systemic sclerosis patients and healthy controls only in non-smokers. However, serum Clara cell 16-kDa protein concentrations were significantly elevated in patients with high resolution computed tomography extent >20% in comparison to patients with high resolution computed tomography extent
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- 2016
31. Níveis séricos de proteína de células de Clara de 16kDa e comprometimento pulmonar em pacientes com esclerose sistêmica
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Anna Olewicz-Gawlik, Aleksandra Dańczak-Pazdrowska, Halina Batura-Gabryel, Dorota Trzybulska, Barbara Kuznar-Kaminska, Katarzyna Katulska, and Paweł Hrycaj
- Subjects
030203 arthritis & rheumatology ,Gynecology ,medicine.medical_specialty ,Doença intersticial pulmonar ,business.industry ,Interstitial lung disease ,03 medical and health sciences ,0302 clinical medicine ,030228 respiratory system ,Rheumatology ,Esclerose sistêmica ,Medicine ,Systemic sclerosis ,business ,CC16 - Abstract
ResumoObjetivoAvaliar a utilidade clínica das medições séricas da proteína de células de Clara de 16‐kDa (CC16) em relação ao sistema de estadiamento para doença pulmonar intersticial associada a esclerose sistêmica (DPI‐ES).Materiais e métodosForam determinados os níveis séricos de CC16 por ELISA em 28 pacientes com ES e 30 controles saudáveis e correlacionados com o sistema de estadiamento para DPI‐ES em pacientes com ES. O envolvimento pulmonar foi avaliado funcionalmente (pletismografia corporal, capacidade de difusão de monóxido de carbono) e radiologicamente (extensão média da doença na tomografia computadorizada de alta resolução dos pulmões, TCAR) em pacientes com ES.ResultadosForam encontradas diferenças estatisticamente significativas nos níveis séricos de CC16 entre pacientes com ES e controles saudáveis apenas em não tabagistas. No entanto, as concentrações séricas de CC16 eram significativamente elevadas em pacientes com extensão>20% na TCAR em comparação com pacientes com extensão 20% in comparison to patients with HRCT extent
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- 2016
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32. Oral mucosal manifestations in primary and secondary Sjögren syndrome and dry mouth syndrome
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Adriana Polańska, Anna Olewicz-Gawlik, Jarosław Kocięcki, Henryk Witmanowski, Katarzyna Błochowiak, Michalina Nowak-Gabryel, and Jerzy Sokalski
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lcsh:Internal medicine ,Saliva ,Pathology ,medicine.medical_specialty ,Dermatology ,Sjögren syndrome ,Oral cavity ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,lcsh:Dermatology ,medicine ,Immunology and Allergy ,dry mouth syndrome ,lcsh:RC31-1245 ,030203 arthritis & rheumatology ,Original Paper ,Secondary Sjögren Syndrome ,business.industry ,030206 dentistry ,lcsh:RL1-803 ,Dry mouth ,medicine.disease ,stomatognathic diseases ,medicine.symptom ,business - Abstract
Introduction : One of the most important symptoms of Sjögren syndrome is xerostomia. The oral cavity deprived of saliva and its natural lubricative, protective and antibacterial properties is prone to a number of unfavourable consequences. Aim : To present the most important lesions on the oral mucosa in primary and secondary Sjögren syndrome and in dry mouth syndrome. Material and methods: The study group comprised 55 patients including 52 women and 3 men aged 20–72 years (average: 28.25 years). Results : Basing on the accepted criteria, primary Sjögren syndrome was diagnosed in 22 (40%) patients, secondary Sjögren syndrome in 18 (32.7%) patients, and dry mouth syndrome in 15 (27.27%) patients. The physical examination and the examination of the mouth were performed and history was elicited from every patient. Conclusions : The most common pathologies appearing on the oral mucosa in primary and secondary Sjögren syndrome are angular cheilitis, cheilitis, increased lip dryness as well as non-specific ulcerations, aphthae and aphthoid conditions.
- Published
- 2016
33. The effect of caveolin-1 knockdown on interleukin-1β-induced chemokine (C-C motif) ligand 2 expression in synovial fluid-derived fibroblast-like synoviocytes from patients with rheumatoid arthritis
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Dorota Trzybulska, Jan Sikora, Mariusz Kaczmarek, Paweł Hrycaj, Agata Kolecka-Bednarczyk, Anna Olewicz-Gawlik, and Magdalena Frydrychowicz
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Chemokine ,Small interfering RNA ,Caveolin 1 ,Interleukin-1beta ,Medicine (miscellaneous) ,Down-Regulation ,CCL2 ,030226 pharmacology & pharmacy ,General Biochemistry, Genetics and Molecular Biology ,Arthritis, Rheumatoid ,03 medical and health sciences ,0302 clinical medicine ,Synovial Fluid ,Internal Medicine ,medicine ,Synovial fluid ,Humans ,Pharmacology (medical) ,Genetics (clinical) ,Cells, Cultured ,Chemokine CCL2 ,Gene knockdown ,biology ,Chemistry ,Monocyte ,Synovial Membrane ,Interleukin ,Fibroblasts ,Molecular biology ,Synoviocytes ,medicine.anatomical_structure ,Cell culture ,030220 oncology & carcinogenesis ,Reviews and References (medical) ,biology.protein - Abstract
Background Rheumatoid arthritis (RA) is a chronic autoimmune disease leading to destructive changes in peripheral joints and their irreversible deformity. The influx of chemoattractant-mediated inflammatory cells to the joints is one of the main features of RA. Objectives The aim of this study was to investigate the effect of a knockdown of caveolin-1 (CAV1), a known regulator of multiple cell signaling pathways, on chemokine (C-C motif) ligand 2/monocyte chemoattractant protein-1 (CCL2/MCP-1) expression in synovial fluid-derived fibroblast-like synoviocytes (sfd-FLSs) obtained from patients with RA. Material and methods Primary cell cultures of sfd-FLSs were established from RA synovial fluids. Cells were transiently transfected with small interfering RNA (siRNA) specific for CAV1, and then incubated with interleukin (IL)-1β to induce CCL2 expression. The expression levels of CAV1 and CCL2 were assessed at transcript level, using quantitative polymerase chain reaction (qPCR) and at protein level by enzyme-linked immunosorbent assay (ELISA) and western blotting analysis. Results A transient CAV1 knockdown in sfd-FLSs resulted in a decrease in the IL-1β-induced CCL2 mRNA expression level vs non-transfected cells and cells transfected with non-targeting siRNA. The concentration of secreted CCL2 was not affected significantly. Conclusions Our study demonstrates that CCL2 expression in sfd-FLSs is CAV1-dependent, but only at transcript level. As the function of CAV1 has not been unequivocally determined, more studies are needed to confirm the role of CAV1 in inflammatory processes related to RA.
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- 2018
34. Skin Barrier Function in Patients with Primary and Secondary Sjögren's Syndrome
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Anna, Olewicz-Gawlik, Adriana, Polańska, Dorota, Trzybulska, Michalina, Nowak-Gabryel, Katarzyna, Błochowiak, Jarosław, Kocięcki, Jerzy, Sokalski, Ryszard, Żaba, Zygmunt, Adamski, and Aleksandra, Dańczak-Pazdrowska
- Subjects
Adult ,Sjogren's Syndrome ,Case-Control Studies ,Humans ,Female ,Organism Hydration Status ,Middle Aged ,Water Loss, Insensible ,Skin - Abstract
Sjögren's syndrome (SS) is a systemic autoimmune disease. A large percentage of patients with SS suffer from dry skin, the cause and pathogenesis of which in this group of patients remains obscure. The aim of the present study was to investigate skin barrier function in patients with SS. Measurements of transepidermal water loss (TEWL) and hydration of stratum corneum (corneometry, CM) were performed in 30 female patients with SS (17 with primary SS and 13 with secondary SS), 20 patients with atopic dermatitis (AD) and 14 healthy controls. There were no statistically significant differences in TEWL values between the three investigated groups, while CM values were significantly decreased in patients with AD when compared with patients with SS and the healthy controls. Based on the obtained results, skin barrier function and hydration in patients with SS showed no functional alterations.
- Published
- 2018
35. Skin barrier function in primary and secondary Sjögren’s syndrome patients
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Anna Olewicz-Gawlik, Adriana Polańska, Dorota Trzybulska, Michalina Nowak-Gabryel, Katarzyna Błochowiak, Jarosław Kocięcki, Jerzy Sokalski, Ryszard Żaba, Zygmunt Adamski, and Aleksandra Dańczak-Pazdrowska
- Subjects
integumentary system - Abstract
Sjögren’s syndrome (SS) is a systemic autoimmune disease. A large percentage of patients with SS suffer from dry skin, the cause and pathogenesis of which in this group of patients remains obscure. The aim of the present study was to investigate skin barrier function in patients with SS. Measurements of transepidermal water loss (TEWL) and hydration of stratum corneum (corneometry, CM) were performed in 30 female patients with SS (17 with primary SS and 13 with secondary SS), 20 patients with atopic dermatitis (AD) and 14 healthy controls. There were no statistically significant differences in TEWL values between the three investigated groups, while CM values were significantly decreased in patients with AD when compared with patients with SS and the healthy controls. Based on the obtained results, skin barrier function and hydration in patients with SS showed no functional alterations.
- Published
- 2018
36. A case of bizarre posttransplant anti-glomerular basement membrane disease
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Olewicz-Gawlik, Anna, primary, Żeromski, Jan, additional, Świerczewska, Monika, additional, Idasiak-Piechocka, Ilona, additional, Pawlik, Magdalena, additional, Sikorska, Dorota, additional, Samara, Husam, additional, Oko, Andrzej, additional, Sikora, Jan, additional, and Dworacki, Grzegorz, additional
- Published
- 2019
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37. Circulating adipokines and organ involvement in patients with systemic sclerosis
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Olewicz-Gawlik, A., Danczak-Pazdrowska, A., Kuznar-Kaminska, B., Batura-Gabryel, H., Katulska, K., Wojciech, S., Trzybulska, D., and Hrycaj, P.
- Subjects
Adult ,Leptin ,Male ,lcsh:Immunologic diseases. Allergy ,lcsh:Internal medicine ,Scleroderma, Systemic ,scleroderma, adipokines, pulmonary fibrosis ,Pilot Projects ,Middle Aged ,respiratory system ,Young Adult ,Humans ,Female ,Resistin ,Adiponectin ,lcsh:RC31-1245 ,lcsh:RC581-607 ,hormones, hormone substitutes, and hormone antagonists ,Aged - Abstract
In recent years, mediators synthesized in the adipose tissue, the so-called adipokines, have been reported to play important roles in the pathogenesis of autoimmune rheumatic diseases.To compare serum leptin, adiponectin and resistin levels in patients with systemic sclerosis (SSc) and healthy controls. To find possible relationship between serum levels of adipokines and organ involvement with focus on interstitial lung disease in SSc patients.Lung involvement was assessed functionally (body plethysmography, diffusing capacity of the lung for carbon monoxide (DLCO) and six-minute walk test) and radiologically (using average disease extent on high resolution computed tomography (HRCT) of the lungs according to the percentage of interstitial changes) in 29 SSc patients. Quantitative sandwich ELISA was used to measure resistin, leptin and adiponectin concentrations in sera of patients and 30 healthy controls.We found no statistically significant differences in serum resistin, leptin and adiponectin levels between SSc patients and the controls. However, serum adiponectin concentrations were significantly lower in active than in inactive patients, they also correlated positively with vital capacity (VC) (p=0.04) and negatively with Valentini activity score (p=0.04). Serum resistin levels were significantly elevated in patients with digital ulcers (p=0.03) and serum concentrations of leptin were associated with the duration of SSc symptoms other than Raynaud's phenomenon (p0.01) CONCLUSIONS: Serum adiponectin should be further investigated as a candidate for SSc activity marker and resistin may play a role in ulcer development in SSc patients.
- Published
- 2015
38. Soluble selectins and highly fucosylated ?1-antichymotrypsin in rheumatoid arthritis patients
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Izabela Korczowska‑Łącka, Anna Olewicz‑Gawlik, and Paweł Hrycaj
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rheumatoid arthritis ,medicine.medical_specialty ,selectins ,biology ,business.industry ,Acute-phase protein ,medicine.disease ,Leukocyte extravasation ,Endocrinology ,Internal medicine ,Rheumatoid arthritis ,acute phase proteins ,fucosylation ,biology.protein ,Medicine ,Rheumatoid factor ,α1 antichymotrypsin ,Antibody ,business ,Fucosylation ,Selectin - Abstract
Introduction. Fucosylation of acute phase proteins and serum soluble selectin levels is increased in rheumatoid arthritis (RA) patients and can influence leukocyte extravasation. Aim. The aim of this study was to evaluate the concentration and fucosylation of ?1-antichymotrypsin (ACT) in relation to serum concentrations of soluble forms of selectins in RA patients. Material and methods. Serum samples of 70 RA patients and 30 healthy controls were examined using sandwich enzyme-linked immunosorbent assay (ELISA). Results. ACT-FR was significantly increased in RA patients when compared to healthy controls (p
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- 2015
39. Dialysis vintage and cardiovascular injury as factors influencing long-term survival in peritoneal dialysis and hemodialysis
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Patrycja Klysz, Dorota Sikorska, Magdalena Roszak, Katarzyna Ścigacz, Anna Olewicz-Gawlik, Bengt Lindholm, Andrzej Oko, Krzysztof Hoppe, Anna Kawka, Krzysztof Schwermer, Ewa Baum, and Krzysztof Pawlaczyk
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Adult ,Male ,medicine.medical_specialty ,Time Factors ,medicine.medical_treatment ,030232 urology & nephrology ,Medicine (miscellaneous) ,General Biochemistry, Genetics and Molecular Biology ,Peritoneal dialysis ,03 medical and health sciences ,0302 clinical medicine ,Troponin T ,Renal Dialysis ,Risk Factors ,Internal medicine ,Cause of Death ,Internal Medicine ,medicine ,Humans ,Pharmacology (medical) ,030212 general & internal medicine ,Myocardial infarction ,Stroke ,Genetics (clinical) ,Dialysis ,Aged ,Ejection fraction ,business.industry ,Middle Aged ,medicine.disease ,Survival Analysis ,Survival Rate ,Cardiovascular Diseases ,Reviews and References (medical) ,Cardiology ,Kidney Failure, Chronic ,Cardiovascular Injury ,Female ,Hemodialysis ,business ,Peritoneal Dialysis ,Biomarkers - Abstract
BACKGROUND Cardiovascular (CV) incidents are the major cause of mortality in maintenance dialysis (MD) patients undergoing peritoneal dialysis (PD) or hemodialysis (HD). CV injury indicators may be useful to investigate the dialysis modality influence on survival. OBJECTIVES The aim of this study was to compare selected laboratory and echocardiographic (ECHO) markers of CV injury in terms of dialysis vintage (DV), CV-related mortality and all-cause mortality. MATERIAL AND METHODS The study involved 301 patients on HD (n = 301) and PD (n = 58), who were divided into subgroups according to DV. The subjects' medical histories included diabetes mellitus (DM), myocardial infarction (MI), stroke, CV deaths and deaths from non-CV causes. Their CV parameters were measured with ECHO for the left ventricle ejection fraction (EF), posterior wall (LVW) and interventricular septum (IVS). Serum analyses of cardiac troponin T (TnT) and N-terminal pro-brain natriuretic peptide (BNP) were also carried out. RESULTS In the subgroup with a DV of 4 years, the PD and HD patients were of a similar age, and had similar mortality and morbidity rates and CV markers, except for thicker IVS in the HD patients. CONCLUSIONS Focusing on the data analysis based on mortality, and both laboratory and echocardiographic markers of cardiovascular injury, PD seems to be a more favorable method of dialysis. The advantage of PD was noted in subjects with a DV < 2 years. HD showed no outcome benefit over PD in longer DV.
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- 2017
40. Patterns of glomerular disease based on 4-year kidney biopsy material analyzed by light microscopy and immunofluorescence: a retrospective single-center analysis in Poland
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Andrzej Oko, Anna Olewicz-Gawlik, Katarzyna Iwanik, Jan Żeromski, Dorota Sikorska, Jan Sikora, Krzysztof Pawlaczyk, Ilona Idasiak-Piechocka, Agnieszka Pluto-Prądzyńska, Magdalena Roszak, Aldona Woźniak, and Grzegorz Dworacki
- Subjects
Nephrology ,Adult ,Male ,medicine.medical_specialty ,Pathology ,Adolescent ,Kidney Glomerulus ,Lupus nephritis ,lcsh:Medicine ,Fluorescent Antibody Technique ,Pathology and Forensic Medicine ,Young Adult ,Focal segmental glomerulosclerosis ,Membranous nephropathy ,renal biopsy ,Internal medicine ,Membranoproliferative glomerulonephritis ,medicine ,Humans ,Aged ,Retrospective Studies ,Aged, 80 and over ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Glomerulonephritis ,General Medicine ,Middle Aged ,medicine.disease ,epidemiology ,Female ,Kidney Diseases ,Renal biopsy ,Poland ,business ,Nephrotic syndrome ,glomerulonephritis - Abstract
The aim of this study was to assess the epidemiology of different patterns of chronic glomerular diseases based on clinical, histopathological and immunofluorescent findings of glomerulonephritis patients hospitalized in the Department of Nephrology, Transplantology and Internal Diseases in Poznan between January 2009 and December 2012. We retrospectively studied 418 patients who had been subjected to renal biopsies. Data on serum creatinine concentration, 24 h proteinuria, arterial hypertension, diabetes mellitus, and histological and immunofluorescent findings were collected. The patients’ mean age was 42 ±15. The male sex prevailed (53.1%). Immunoglobulin A nephropathy was the most common finding (18.9%), followed by focal segmental glomerulosclerosis (16.3%), membranous glomerulonephritis (10.1%), lupus nephritis (8.4%), extracapillary glomerulonephritis (3.3%) and membranoproliferative glomerulonephritis (2.6%). In 69 (16.5%) patients the biopsy was non-informative or non-diagnostic. Patients with membranous nephropathy presented the highest frequency of nephrotic syndrome (71.4%), followed by membranoproliferative glomerulonephritis and focal segmental glomerulosclerosis. Combined analysis of the clinical, histopathological and immunofluorescent findings in glomerulonephritis patients based on a single center’s data can provide important epidemiological findings.
- Published
- 2017
41. Factors associated with overhydration in peritoneal dialysis patients
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Krzysztof Pawlaczyk, Dorota Sikorska, Andrzej Oko, Anna Olewicz-Gawlik, Ewa Baum, Magdalena Roszak, Małgorzata Kałużna, Maria Wanic-Kossowska, Bengt Lindholm, and Włodzimierz Samborski
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,030232 urology & nephrology ,state of hydration ,Diuresis ,Renal function ,Peritoneal equilibration test ,030204 cardiovascular system & hematology ,Logistic regression ,Gastroenterology ,Peritoneal dialysis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Diabetes mellitus ,medicine ,bioelectrical impedance analysis ,business.industry ,medicine.disease ,Surgery ,peritoneal dialysis ,Medicine ,business ,Hypervolemia ,Bioelectrical impedance analysis - Abstract
Aim. Overhydration is a prevalent problem in peritoneal dialysis (PD) patients. The aim of the study was to evaluate the effect of several factors on the development of overhydration in PD patients. Material and Methods. The study was performed on 74 PD patients, who were divided into two groups according to bioimpedance analysis hydration status (OHBIA): Group A OHBIA
- Published
- 2017
42. The importance of hypoalbuminemia in peritoneal dialysis patients: Impact of gender
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Sikorska, Dorota, primary, Olewicz-Gawlik, Anna, additional, Baum, Ewa, additional, Pawlaczyk, Krzysztof, additional, and Oko, Andrzej, additional
- Published
- 2018
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43. Theeffect of caveolin-1 knockdown on interleukin-1β-induced chemokine (C-C motif) ligand 2 expression in synovial fluid-derived fibroblast-like synoviocytes from patients with rheumatoid arthritis
- Author
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Trzybulska, Dorota, primary, Olewicz-Gawlik, Anna, additional, Sikora, Jan, additional, Frydrychowicz, Magdalena, additional, Kolecka-Bednarczyk, Agata, additional, Kaczmarek, Mariusz, additional, and Hrycaj, Pawel, additional
- Published
- 2018
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44. Levels of EGF and VEGF in patients with primary and secondary Sjögren’s syndrome
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Błochowiak, Katarzyna, primary, Trzybulska, Dorota, additional, Olewicz-Gawlik, Anna, additional, Sikora, Jan, additional, Nowak-Gabryel, MIchalina, additional, Kocięcki, Jarosław, additional, Witmanowski, Henryk, additional, and Sokalski, Jerzy, additional
- Published
- 2018
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45. Transforming growth factor-β1 in plaque morphea
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Justyna Gornowicz-Porowska, Anna Olewicz-Gawlik, Aleksandra Dańczak-Pazdrowska, Beata Szramka-Pawlak, Wojciech Silny, Michał J. Kowalczyk, Ryszard Żaba, Jakub Pazdrowski, Aleksandra Szewczyk, Marta Molińska-Glura, and Paweł Hrycaj
- Subjects
Original Paper ,transforming growth factor ,Pathology ,medicine.medical_specialty ,business.industry ,morphea ,transforming growth factor-β ,Dermatology ,medicine.disease ,Peripheral blood mononuclear cell ,Scleroderma ,Pathogenesis ,Breast cancer ,Fibrosis ,Immunology ,Immunology and Allergy ,Medicine ,scleroderma ,business ,Localized Scleroderma ,Morphea ,Transforming growth factor - Abstract
Introduction: Morphea (localized scleroderma) is a rare cutaneous disease characterized by skin fibrosis of unknown pathogenesis. Transforming growth factor-β (TGF-β) is a potent profibrotic factor. The role of TGF-β in morphea remains unclear. Aim: The goal of this study was to estimate the expression level of TGF-β1 in skin and peripheral blood mononuclear cells as well as the plasma levels of TGF-β1 in plaque morphea (MEP). Material and methods: The study involved 20 MEP patients. Three control groups were involved: 1 - plasma: 36 healthy volunteers; 2 - PBMC: 47 healthy volunteers; 3 - skin biopsies: 13 samples collected during mastectomy (breast cancer was not skin involved). The analysis of TGF-β1 plasma levels was performed with the use an adequate ELISA kit, while real-time polymerase chain reaction was employed for the expression of TGF-β1 in peripheral blood mononuclear cells (PBMC) and skin. Results: In our study we have not detected differences in TGF-β 1 expression in PBMC, skin, nor in plasma levels of TGF-β1 between MEP patients and healthy controls, regardless of disease activity and its duration. Conclusions: The results of our study contradict the claim of the substantial role of TGF-β1 in the most common morphea subtype - MEP.
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- 2013
46. Clinical immunology Interleukin 1β in morphea
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Michał J. Kowalczyk, Justyna Gornowicz-Porowska, Marta Molińska-Glura, Ryszard Żaba, Wojciech Silny, Beata Szramka-Pawlak, Paweł Hrycaj, Anna Olewicz-Gawlik, Aleksandra Dańczak-Pazdrowska, and Aleksandra Szewczyk
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Interleukin 1β ,Clinical immunology ,business.industry ,Immunology ,medicine ,Immunology and Allergy ,medicine.disease ,business ,Scleroderma ,Morphea - Published
- 2012
47. Interleukin-17A and interleukin-23 in morphea
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Anna Olewicz-Gawlik, Aleksandra Dańczak-Pazdrowska, Paweł Hrycaj, Beata Szramka-Pawlak, Justyna Gornowicz-Porowska, Aleksandra Szewczyk, Michał J. Kowalczyk, Ryszard Żaba, Wojciech Silny, and Marta Molińska-Glura
- Subjects
business.industry ,morphea ,medicine.medical_treatment ,Interleukin ,General Medicine ,medicine.disease ,Peripheral blood mononuclear cell ,Pathogenesis ,Cytokine ,Clinical Research ,Immunology ,Gene expression ,Interleukin 23 ,medicine ,scleroderma ,interleukin 23 ,autoimmune diseases ,Interleukin 17 ,interleukin 17 ,business ,Morphea - Abstract
Introduction: Morphea is a disease included in the group of scleroderma type autoimmune diseases. Interleukin (IL)-17A may play a role at every stage of its pathogenesis. The study aimed at evaluation of IL-17A and IL-23 (as the main cytokine which is supposed to stimulate and maintain synthesis of IL-17) in pathogenesis of morphea. Material and methods: The studies were performed on 41 blood samples from patients with morphea. Skin was sampled from 29 patients. The evaluation included: (1) expression of IL-17A and IL-23 genes in peripheral blood mononu clear cells (PBMC) using real-time polymerase chain reaction (PCR), (2) plasma concentrations of IL-17A and IL-23 using ELISA, (3) expression of IL-17A and IL- 23 genes in skin using real-time PCR. Results: The results of gene expression are expressed as median number of copies per million copies of GAPDH. Higher expression of IL-17A has been demonstrated in PBMC of morphea vs. control group (2630 and 1906 respectively; p = 0.004), accompanied by absence of significant differences in its plasma concentration (10 pg/ml in both groups) and by lowered expression in affected skin (9119 and 19113 respectively; p = 0.036). The results failed to demonstrate elevated IL-23 plas ma concentration in morphea vs. control group (5 pg/ml and 6 pg/ml respective ly; p = 0.335) or its increased expression in the skin (292 vs. 427; p = 0.383), although we noted its increased expression in PBMC (4419 vs. 808; p < 0.001). Conclusions: Based on the observed correlations we suggest that: (1) IL-17A does not represent a factor which promotes tissue injury in morphea, (2) IL-23 may playa role in pathogenesis of morphea.
- Published
- 2012
48. Blood serum levels of amino-terminal pro-C-type natriuretic peptide in patients with rheumatoid arthritis
- Author
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Anna Olewicz-Gawlik, Paweł Hrycaj, Paweł Grala, and Dorota Trzybulska
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Adult ,Male ,medicine.medical_specialty ,Endothelium ,medicine.drug_class ,Arthritis ,Enzyme-Linked Immunosorbent Assay ,Arthritis, Rheumatoid ,Pathogenesis ,Blood serum ,Internal medicine ,Synovitis ,medicine ,Natriuretic peptide ,Humans ,Endothelial dysfunction ,Aged ,business.industry ,Natriuretic Peptide, C-Type ,General Medicine ,Middle Aged ,medicine.disease ,Endocrinology ,medicine.anatomical_structure ,Rheumatoid arthritis ,Selectins ,Female ,business - Abstract
Angiogenesis is a prominent feature of rheumatoid synovitis and contributes to perpetuation of joint disease. The aim of presented study was to evaluate the association of inflammatory response and endothelial dysfunction parameters with serum levels of amino-terminal pro-C-type natriuretic peptide (NT-proCNP), a surrogate measure for a potent regulator of vascular tone and angiogenesis, CNP.We included in this study 40 rheumatoid arthritis (RA) patients (36 female, four male) and 30 healthy controls (28 female, two male). Disease activity score (DAS28), Health Assessment Questionnaire Disability Index (HAQDI), Rheumatoid Arthritis Disease Activity Index (RADAI) and visual analogue scales (VAS) for pain and arthritis were determined. The concentrations of the following laboratory parameters (serum C-reactive protein (CRP), soluble (s) L-, sP-, sE-selectin, NT-proCNP, IgM rheumatoid factor (RF), and anti-cyclic citrullinated peptide antibodies) were measured using sandwich enzyme-linked immunosorbent assay (ELISA).The concentrations of NT-proCNP in RA patients were significantly increased when compared to healthy controls (p0.001). In RA patients we found significant correlations only between sE-selectin and NT-proCNP levels (p=0.02). No significant associations were found between serum NT-proCNP and soluble selectin levels and patients' age and sex. Also, the parameters of clinical profile in the RA group (HAQ, RADAI, DAS28, VAS-pain, VAS-arthritis, swollen and tender joint counts) showed no association with serum NT-proCNP, sE-, sP- and sL-selectin.Serum concentration of NT-proCNP reflecting serum CNP level is likely secondary to the activation of endothelium. Nevertheless, the results of our study encourage to further studies over the role of CNP in the pathogenesis of RA, preferably on tissue level.
- Published
- 2010
49. Blood Serum Levels of Amino-Terminal Pro-C-Type Natriuretic Peptide in Patients with Systemic Sclerosis
- Author
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S. H. Mackiewicz, K Klama, Anna Olewicz-Gawlik, Aleksandra Dańczak-Pazdrowska, Paweł Grala, Wojciech Silny, Janusz Prokop, and Paweł Hrycaj
- Subjects
Male ,medicine.medical_specialty ,Endothelium ,medicine.drug_class ,Health Status ,Amino terminal ,Pilot Projects ,Inflammation ,Severity of Illness Index ,Biochemistry ,Blood serum ,Rheumatology ,Internal medicine ,medicine ,Natriuretic peptide ,Humans ,Orthopedics and Sports Medicine ,In patient ,skin and connective tissue diseases ,Molecular Biology ,Skin ,Scleroderma, Systemic ,integumentary system ,business.industry ,Significant difference ,Natriuretic Peptide, C-Type ,Cell Biology ,Middle Aged ,P-Selectin ,C-Reactive Protein ,Endocrinology ,medicine.anatomical_structure ,Disease Progression ,Female ,medicine.symptom ,E-Selectin ,business ,Biomarkers ,Selectin - Abstract
The aim of this pilot study was to examine the association of serum levels of amino-terminal fragment of pro-C-type natriuretic peptide (NT-proCNP), as a substitute measure of serum C-type natriuretic peptide (CNP), with clinical and laboratory findings in patients with systemic sclerosis (SSc). Serum NT-proCNP, soluble (s)E-and sP-selectin levels were examined using specific enzyme-linked immunosorbent assay in 30 patients with SSc and 30 healthy controls. We found no statistically significant difference in serum levels of NT-proCNP neither between patients with SSc and healthy controls nor between those treated with immunosuppressive agents and untreated patients. Nevertheless, in the investigated SSc group, serum NT-proCNP levels correlated with the concentrations of C-reactive protein (CRP) and the duration of the disease. Both sP- and sE-selectin levels were elevated in SSc patients when compared to healthy subjects. Also they did not correlate with the concentrations of NT-proCNP. The results of the study indicate that serum NT-proCNP level is likely secondary to existing inflammation. However, the magnitude of CNP action in SSc and its possible role in the pathogenesis of the disease remains to be elucidated.
- Published
- 2009
50. Serum ICAM-1, VCAM-1 and E-selectin levels in patients with primary and secondary Sjögren’s syndrome
- Author
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Błochowiak, Katarzyna, primary, OLEWICZ-GAWLIK, ANNA, additional, Trzybulska, Dorota, additional, Nowak-Gabryel, Michalina, additional, Kocięcki, Jarosław, additional, Witmanowski, Henryk, additional, and Sokalski, Jerzy, additional
- Published
- 2017
- Full Text
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