629 results on '"Oliveira JS"'
Search Results
2. The Value of Mind-Body Connection in Physical Activity for Older People
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Gilchrist, H, Haynes, A, Oliveira, JS, Grunseit, A, Sherrington, C, Bauman, A, Shepherd, R, and Tiedemann, A
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1106 Human Movement and Sports Sciences ,Gerontology - Abstract
Exercise that targets balance and strength is proven to prevent falls in older age. The Successful AGEing yoga trial is the first large randomized controlled trial to assess the impact of yoga on falls in people aged ≥60 years. We conducted a realist process evaluation to explain the strong participant engagement observed using interviews (21 participants and three yoga instructors) and focus groups (12 participants and four yoga instructors). Results showed that relaxation, breathing, and yoga's mind-body connection created a satisfying internal focus on bodily sensation which was valued by participants. The mechanisms of mindfulness and embodiment appeared to facilitate this. Mindfulness and embodiment are also linked to, and enhance engagement with, other forms of physical activity. By focusing creatively on these mechanisms, we can develop a range of programs that target improvements in physical and mental health (including reducing falls and fear of falls) and appeal to older people.
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- 2022
3. Yoga-based exercise to prevent falls in community-dwelling people aged 60 years and over: Study protocol for the Successful AGEing (SAGE) yoga randomised controlled trial
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Oliveira, JS, Sherrington, C, Lord, S, Sesto, R, Youkhana, S, Camara, GC, Grunseit, AC, Bauman, A, Anstey, KJ, Shepherd, RB, Tiedemann, A, Oliveira, JS, Sherrington, C, Lord, S, Sesto, R, Youkhana, S, Camara, GC, Grunseit, AC, Bauman, A, Anstey, KJ, Shepherd, RB, and Tiedemann, A
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Introduction Falls significantly reduce independence and quality of life in older age. Balance-specific exercise prevents falls in people aged 60+ years. Yoga is growing in popularity and can provide a high challenge to balance; however, the effect of yoga on falls has not been evaluated. This trial aims to establish the effect on falls of a yoga exercise programme compared with a yoga relaxation programme in community-dwellers aged 60+ years. Method and Analysis This randomised controlled trial will involve 560 community-dwelling people aged 60+ years. Participants will be randomised to either: (1) the Successful AGEing (SAGE) yoga exercise programme or (2) a yoga relaxation programme. Primary outcome is rate of falls in the 12 months post randomisation. Secondary outcomes include mental well-being, physical activity, health-related quality of life, balance self-confidence, physical function, pain, goal attainment and sleep quality at 12 months after randomisation. The number of falls per person-year will be analysed using negative binomial regression models to estimate between-group difference in fall rates. Generalised linear models will assess the effect of group allocation on the continuously scored secondary outcomes, adjusting for baseline scores. An economic analysis will compare the cost-effectiveness and cost-utility of the two yoga programmes. Ethics and dissemination Protocol was approved by the Human Research Ethics Committee at The University of Sydney, Australia (approval 2019/604). Trial results will be disseminated via peer-reviewed articles, conference presentations, lay summaries. Trial registration number The protocol for this trial is registered with the Australian New Zealand Clinical Trials Registry (ACTRN12619001183178).
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- 2020
4. OASIS-a randomised, placebo-controlled trial of oral glucocorticoids for leg pain in patients with acute sciatica: trial protocol
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Liu, C, Abdel Shaheed, C, McLachlan, AJ, Latimer, J, Li, Q, Buchbinder, R, Day, RO, Maher, CG, Richards, B, Oliveira, JS, Lin, CWC, Liu, C, Abdel Shaheed, C, McLachlan, AJ, Latimer, J, Li, Q, Buchbinder, R, Day, RO, Maher, CG, Richards, B, Oliveira, JS, and Lin, CWC
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INTRODUCTION: Sciatica is a lower spine condition characterised by radiating leg pain below the knee. It may be accompanied by motor and sensory loss in the distribution of a spinal nerve. There are few effective treatments for sciatica. Orally administered glucocorticoids have shown some promise, however, any beneficial effects need to be confirmed and weighed against drug safety and cost-effectiveness, in a high-quality, definitive trial. METHODS AND ANALYSIS: The Oral Steroids In Sciatica (OASIS) trial is a randomised, placebo-controlled, double-blind trial that will evaluate a tapering regimen of oral prednisolone in 200 participants with acute sciatica. Participants will be recruited on presentation to general practice, specialist outpatient clinics or hospital emergency departments and randomised to receive orally administered prednisolone 50 mg per day, up to 3 days then tapering to cessation over 10 days, or placebo, for a maximum of 13 days, in addition to guideline advice. Participants will be followed for 1 year. The primary endpoint will be leg pain intensity at 2 weeks. Secondary outcomes will include back pain intensity, disability, time to recovery, quality of life and treatment success rate. Adverse events will be assessed and a cost-effectiveness analysis will be conducted. ETHICS AND DISSEMINATION: Ethical approval has been granted from the Human Research Ethics Committee, The University of Sydney. Trial results will be disseminated by publications and conference presentations and via the media. TRIAL REGISTRATION NUMBER: ACTRN12619001716156.
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- 2020
5. Frequency of SNP -336A/G in the promoter region of CD209 in a population from northeastern Brazil
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Pereira Ac, P.N. Costa, Giovanny R. Pinto, de Oliveira Js, Ferreira Gp, and Hygor Ferreira-Fernandes
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Male ,Genotype ,Population ,Receptors, Cell Surface ,Biology ,Polymorphism, Single Nucleotide ,law.invention ,Gene Frequency ,law ,Genetics ,Humans ,SNP ,Lectins, C-Type ,Allele ,Promoter Regions, Genetic ,education ,Molecular Biology ,Allele frequency ,Alleles ,Polymerase chain reaction ,Aged ,Aged, 80 and over ,education.field_of_study ,Promoter ,General Medicine ,Middle Aged ,Molecular biology ,Genotype frequency ,Genetics, Population ,Female ,Cell Adhesion Molecules ,Brazil - Abstract
Dendritic cells (DCs) mediate the initiation of the immune response against a variety of pathogens. The DC-SIGN receptor is encoded by the gene CD209 and is expressed on the surface of DCs. It binds to mannose-rich carbohydrates and enables the recognition of bacteria, fungi, parasites, and viruses. SNP -336A/G in the promoter region of CD209 influences the expression of the DC-SIGN receptor. Several studies have associated this SNP with an increased susceptibility to infectious diseases and the development of more severe forms of disease. Therefore, the aim of this study was to determine the prevalence of SNP -336A/G in a population from northeastern Brazil. We analyzed 181 individuals from the general population of Parnaíba, Piauí, Brazil, of which 37% were men and 63% were women. SNP -336A/G was detected by polymerase chain reaction and treatment with the restriction enzyme MscI and visualized by electrophoresis on an 8% polyacrylamide gel stained with silver nitrate. Of the individuals analyzed, 116 (64.1%) were homozygous AA, 57 (31.5%) were heterozygous (AG), and 8 (4.4%) were homozygous GG. The allele frequency of -336G was 20.2%. Genotype frequencies were in Hardy-Weinberg equilibrium. To the best of our knowledge, this is the first report to describe the frequency of the CD209 SNP -336A/G in a population in the State of Piauí. Further studies are needed to determine the relationship between this SNP and the vulnerability of this population to major infectious diseases.
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- 2015
6. Long-Term and Sustained Therapeutic Results of a Specific Promonocyte Cell Formulation in Refractory Angina: ReACT (Refractory Angina Cell Therapy) Clinical Update and Cost-Effective Analysis
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Cruz E, Rodrigues de Oliveira Js, Regina Coeli dos Santos Goldenberg, Paul R. Sanberg, Coimbra Ac, Leslie W. Miller, Noedir Antônio Groppo Stolf, Enio Buffolo, Regazzi G, Rodrigues Branco Jn, Janaína José dos Santos Machado, Nelson Americo Hossne, Silvia P. Azevedo, and Adriana Luckow Invitti
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Male ,medicine.medical_specialty ,Time Factors ,Angiogenesis ,Cost-Benefit Analysis ,Population ,Myocardial Ischemia ,Biomedical Engineering ,lcsh:Medicine ,Monocyte-Macrophage Precursor Cells ,Peripheral blood mononuclear cell ,Statistics, Nonparametric ,Angina Pectoris ,Angina ,Cell therapy ,Percutaneous Coronary Intervention ,Surveys and Questionnaires ,Internal medicine ,medicine ,Humans ,Radionuclide Imaging ,education ,Aged ,Transplantation ,education.field_of_study ,business.industry ,Myocardium ,lcsh:R ,Cell Biology ,Canadian Cardiovascular Society ,Length of Stay ,Middle Aged ,medicine.disease ,Surgery ,Clinical trial ,Quality of Life ,Cardiology ,Female ,Stem cell ,business ,Follow-Up Studies - Abstract
Mononuclear stem cells have been studied for their potential in myocardial ischemia. In our previous published article, ReACT® phase I/II clinical trial, our results suggest that a certain cell population, promonocytes, directly correlated with the perceived angiogenesis in refractory angina patients. This study is ReACT's clinical update, assessing long-term sustained efficacy. The ReACT phase IIA/B noncontrolled, open-label, clinical trial enrolled 14 patients with refractory angina and viable ischemic myocardium, without ventricular dysfunction, who were not suitable for myocardial revascularization. The procedure consisted of direct myocardial injection of a specific mononuclear cell formulation, with a certain percentage of promonocytes, in a single series of multiple injections (24—90; 0.2 ml each) into specific areas of the left ventricle. Primary endpoints were Canadian Cardiovascular Society Angina Classification (CCSAC) improvement at the 12-month follow-up and ischemic area reduction (scintigraphic analysis) at the 12-month follow-up, in correlation with ReACT's formulation. A recovery index (for patients with more than 1 year follow-up) was created to evaluate CCSAC over time, until April 2011. Almost all patients presented progressive improvement in CCSAC beginning 3 months ( p = 0.002) postprocedure, which was sustained at the 12-month follow-up ( p = 0.002), as well as objective myocardium ischemic area reduction at 6 months (decrease of 15%, p < 0.024) and 12 months (decrease of 100%, p < 0.004) The recovery index ( n = 10) showed that the patients were graded less than CCSAC 4 for 73.9 ± 24.2% over a median follow-up time of 46.8 months. After characterization, ReACT's promonocyte concentration suggested a positive correlation with CCSAC improvement ( r = −0.575, p = 0.082). Quality of life (SF-36 questionnaire) improved significantly in almost all domains. Cost-effectiveness analysis showed decrease in angina-related direct costs. Refractory angina patients presented a sustained long-term improvement in CCSAC and myocardium ischemic areas after the procedure. The long-term follow-up and strong improvement in quality of life reinforce effectiveness. Promonocytes may play a key role in myocardial neoangiogenesis. ReACT dramatically decreased direct costs.
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- 2015
7. Work-Related Stress and Substance Use as Risk Factors for Chronic Disease Among Three-Wheel Drivers in Galle, Sri Lanka: A Qualitative Study
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Kirkorowicz, JM, primary, Sapukotana, P, primary, De Silva, PV, primary, Noda, M, primary, De Oliveira, JS, primary, and Østbye, T, primary
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- 2014
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8. Prognosis related to staging systems for chronic lymphocytic leukemia
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Marcelo Belchior Rosendo da Silva, Mihoko Yamamoto, de Oliveira Js, José Kerbauy, de Faria, Goihman S, Delbone de Faria Rm, Universidade de Alfenas Division of Hematology, and Universidade Federal de São Paulo (UNIFESP)
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Staging ,Chronic lymphocytic leukemia ,Leucemia linfocítica crônica ,Prognóstico ,lcsh:Medicine ,Context (language use) ,Ethnic origin ,Gastroenterology ,Clinical ,Internal medicine ,hemic and lymphatic diseases ,medicine ,Humans ,Chronic Lymphocytic Leukemia ,Survivors ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Analysis of Variance ,business.industry ,lcsh:R ,Estadiamento ,General Medicine ,Clínica ,Middle Aged ,medicine.disease ,Prognosis ,Leukemia, Lymphocytic, Chronic, B-Cell ,Leukemia ,Female ,business - Abstract
CONTEXTO: Diversas variáveis clínicas se relacionam ao prognóstico na leucemia linfocítica crônica; no entanto, o estadiamento de Binet determina melhor o prognóstico do que o estadiamento de Rai e Rai modificado. OBJETIVO: Avaliar características clínicas e laboratoriais no prognóstico da leucemia linfocítica crônica. TIPO DE ESTUDO: Estudos de correlação. LOCAL: Universidade Federal de São Paulo (UNIFESP) - Escola Paulista de Medicina / Universidade de Alfenas. PARTICIPANTES: 73 patients diagnosticados entre 1977 e 1994. VARIÁVEIS ESTUDADAS: Sexo, origem étnica, idade, linfadenomegalia, esplenomegalia, hepatomegalia, três ou mais áreas com aumento linfóide, hemoglobina, linfocitos, plaquetas. RESULTADOS: A sobrevida média dos pacientes foi 76 meses, idade média de 65 anos, variando de 33 a 87 anos. Quarenta e quatro pacientes (60,3%) eram do sexo masculino e 29 (39,7%) do sexo feminino. A análise univariada mostrou que o nível de hemoglobina (P = 0,001), número de plaquetas (P = 0,03), linfocitose periférica (P = 0,03), número de áreas linfóides acometidas (P = 0,01), assim como os estadiamentos de Rai (P = 0,02), Rai modificado (0,007) e Binet (P = 0,003) relacionaram-se significantemente com a sobrevida. A análise multivariada, pela regressão de Cox, demonstrou que o sistema de Binet determina melhor o prognóstico do que os estadiamentos de Rai e Rai modificado. CONCLUSÃO: O estadiamento de Binet é melhor que o estadiamento de Rai e Rai modificado na determinação do prognóstico. CONTEXT: Chronic lymphocytic leukemia (CLL) is a clonal lymphoproliferative disorder, characterized by B lymphocytic proliferation. CLL is the most frequent adult leukemia in Western countries, accounting for 25 to 30% of all white leukemic patients. OBJECTIVE: To evaluate clinical and staging characteristics in prognosis of chronic lymphocytic leukemia. DESIGN: Evaluation of clinical-staging data. SETTING: Universidade Federal de São Paulo (UNIFESP) - Escola Paulista de Medicina / Universidade de Alfenas. SAMPLE: 73 patients diagnosed from 1977 to 1994. MAIN MEASUREMENTS: Sex, ethnic origin, age, lymphadenopathy, splenomegaly, hepatomegaly, three or more areas of lymphoid enlargement, hemoglobin (g/dl), lymphocytes/mm³, Platelets/mm³ RESULTS: Mean survival of patients was 76 months, median age was 65 years, ranging from 33 to 87. Forty-four patients (60.3%) were male and 29 (39.7%) female. CONCLUSION: The Binet system determined a better prognosis than Rai. Universidade de Alfenas Division of Hematology Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Division of Hematology Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Division of Epidemiology UNIFESP, EPM, Division of Hematology UNIFESP, EPM, Division of Epidemiology SciELO
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- 2000
9. Binding specificity of sea anemone toxins to Nav 1.1-1.6 sodium channels: unexpected contributions from differences in the IV/S3-S4 outer loop
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Oliveira, J, Redaelli, E, Zaharenko, A, Cassulini, R, Konno, K, Pimenta, D, Freitas, J, Clare, J, Wanke, E, Oliveira, JS, Zaharenko, AJ, Cassulini, RR, Pimenta, DC, Freitas, JC, Clare, JJ, REDAELLI, ELISA, WANKE, ENZO, Oliveira, J, Redaelli, E, Zaharenko, A, Cassulini, R, Konno, K, Pimenta, D, Freitas, J, Clare, J, Wanke, E, Oliveira, JS, Zaharenko, AJ, Cassulini, RR, Pimenta, DC, Freitas, JC, Clare, JJ, REDAELLI, ELISA, and WANKE, ENZO
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Sea anemones are an important source of various biologically active peptides, and it is known that ATX-II from Anemonia sulcata slows sodium current inactivation. Using six different sodium channel genes (from Nav1.1 to Nav1.6), we investigated the differential selectivity of the toxins AFT-II (purified from Anthopleura fuscoviridis) and Bc-III (purified from Bunodosoma caissarum) and compared their effects with those recorded in the presence of ATX-II. Interestingly, ATX-II and AFT-II differ by only one amino acid (L36A) and Bc-III has 70% similarity. The three toxins induced a low voltage-activated persistent component primarily in the Nav1.3 and Nav1.6 channels. An analysis showed that the 18 dose-response curves only partially fit the hypothesized binding of Lys-37 (sea anemone toxin Anthopleurin B) to the Asp (or Glu) residue of the extracellular IV/S3-S4 loop in cardiac (or nervous) Na+ channels, thus suggesting the substantial contribution of some nearby amino acids that are different in the various channels. As these channels are atypically expressed in mammalian tissues, the data not only suggest that the toxicity is highly dependent on the channel type but also that these toxins and their various physiological effects should be considered prototype models for the design of new and specific pharmacological tools
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- 2004
10. Increased expression of CD4+CD25 +FOXP3+ regulatory T cells correlates with Epstein-Barr virus and has no impact on survival in patients with classical Hodgkin lymphoma in Brazil.
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Assis MC, Campos AH, Oliveira JS, Soares FA, Silva JM, Silva PB, Penna AD, Souza EM, Baiocchi OC, Assis, Mariane C G, Campos, Antonio H F M, Oliveira, Jose S R, Soares, Fernando A, Silva, Joyce M K, Silva, Priscilla B, Penna, Adriana D, Souza, Eni M, and Baiocchi, Otavio C G
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The tumor microenvironment of classical Hodgkin lymphoma (cHL) is clearly responsible for the maintenance of the malignant Hodgkin-Reed-Sternberg (HRS) cells, and Epstein-Barr virus (EBV) has been shown to play a role in this immune evasion. EBV can increase the migration of CD4(+)CD25(+)FOXP3(+) lymphocytes, named regulatory T cells (Tregs). In this study, we assessed the distribution and biological significance of Tregs in patients with cHL. Tissue microarrays were constructed using diagnostic biopsies available in 130 cHL patients and stained with CD4, CD8, CD25, and FOXP3 antibodies. For the present study, only cHL patients whose histology could be confirmed and EBV association established were studied. From the 130 cHL patients selected for this study, 56 were classified as EBV-related and 74 EBV non-related cHL. There were no association between clinical characteristics and the expression of Tregs. However, higher levels of Tregs correlated with EBV presence on HRS cells (p = 0.02), although it did not influence event-free survival (EFS) and overall survival (p = 0.98 and p = 0.59, respectively). This study demonstrates that Tregs expression correlates with EBV presence in HRS cells and has no impact on survival of patients with cHL. Further studies investigating the mechanisms in which EBV recruits Tregs to the tumor microenvironment will contribute not only to our understanding on the pathogenesis of cHL but also to the development of new therapeutic strategies. [ABSTRACT FROM AUTHOR]
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- 2012
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11. Impact of Epstein-Barr virus in the clinical evolution of patients with classical Hodgkin's lymphoma in Brazil.
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Souza EM, Baiocchi OC, Zanichelli MA, Alves AC, Assis MG, Eiras DP, Dobo C, and Oliveira JS
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INTRODUCTION: Classical Hodgkin's Lymphoma (cHL) has been frequently associated with Epstein-Barr virus (EBV), which can be found in a latent pattern in Reed-Sternberg (RS) cells. However, the impact of the presence of EBV in RS cells and its prognosis are still controversial. We analysed the presence of EBV in RS cells and its influence in the clinical evolution of patients with cHL treated in two public hospitals in the city of Sao Paulo, Brazil. MATERIALS AND METHODS: We selected 97 patients with cHL from 1994 to 2004. Patients were only included in this study if they had (1) >18 years, (2) negative HIV serology, (3) undergone similar chemotherapy protocols, (4) paraffin blocks available with enough material for systematic review and histological reclassification and for detection of EBV in RS cells by in situ hybridization and immunohistochemistry and (5) clinical, epidemiological and laboratorial parameters available after a thorough chart review. RESULTS: EBV was identified in 52.5% of the cases. Mixed cellularity (MC) subtype was more common in EBV-related tumours (25.5%) (p = 0.005). There was no difference on age, gender, stage and the presence of B symptoms between the two groups. The presence of EBV did not influence event free survival (EFS) (p = 0.38) or overall survival (OS) (p = 0.80) with a median follow-up of 80 months. CONCLUSION: We demonstrate that the prevalence of EBV-related cHL in this Brazilian population is 52.5% and, that, the presence of EBV does not change the clinical evolution and OS of patients treated with similar chemotherapy protocols. Copyright © 2010 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]
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- 2010
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12. Detection of galectin-1 and anti-galectin-1 antibodies in serum and cerebrospinal fluid derived from lupus patients
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Delalibera-Joviliano, R., Oliveira, Js, Montes, Mab, Rosa, Jc, Greene, Lj, Donadi, Ea, Cummings, Rd, and Marcelo Dias Baruffi
13. BTC, a human P450 containing Escherichia coli tester strain with a high sensitivity towards alkylating agents
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Duarte, Mp, Palma, Bb, Laires, A., Oliveira, Js, Rueff, J., and Michel Kranendonk
14. Assessing potato canopy growth and development at the individual leaf level to improve the understanding of the plant source–sink relations
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Oliveira, JS, Brown, HE, and Moot, Derrick
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- 2021
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15. Yield and weight distribution of two potato cultivars grown from seed potatoes of different physiological ages
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Oliveira, JS, Brown, HE, Gash, A, and Moot, Derrick
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- 2017
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16. Micronutrient Consumption Among Postpartum Women and Food Insecurity.
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Freire JKS, da Silveira JAC, Longo-Silva G, Silva-Neto LGR, de Andrade MIS, de Lima Soares L, Oliveira JS, de Melo Barros L, de Menezes Marinho P, and de Menezes RCE
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This study aimed to assess the prevalence of inadequate micronutrient intake and its risk factors among women one year after giving birth. 240 women of reproductive age were assessed. Micronutrient intake was assessed from two 24-hour records at three stages (3-6-12 months after childbirth). A generalized linear mixed-effect model was used to assess the factors associated with food intake. A prevalence of inadequate micronutrient intake was observed, with the exception of iron. Intake was lower among food-insecure women (zinc: β -0.13; iron: β -0.09; vitamin C: β -0.44). The postpartum period and food insecurity remained statistically associated with lower food consumption in the explanatory models tested.
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- 2024
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17. Review: Laticifer as a plant defense mechanism.
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Freitas CDT, Demarco D, Oliveira JS, and Ramos MV
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- Plant Defense Against Herbivory, Plants metabolism, Latex metabolism
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Laticifers have been utilized as paradigms to enhance comprehension of specific facets of plant ecology and evolution. From the beginning of seedling growth, autonomous laticifer networks are formed throughout the plant structure, extending across all tissues and organs. The vast majority of identified products resulting from laticifer chemistry and metabolism are linked to plant defense. The latex, which is the fluid contained within laticifers, is maintained under pressure and has evolved to serve as a defense mechanism against both aggressors and invaders, irrespective of their capabilities or tactics. Remarkably, the latex composition varies among different species. The current goal is to understand the specific functions of various latex components in combating plant enemies. Therefore, the study of latex's chemical composition and proteome plays a critical role in advancing our understanding about plant defense mechanisms. Here, we will discuss some of these aspects., Competing Interests: Declaration of Competing Interest No conflicts of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)
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- 2024
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18. Performance of grazing cows fed supplements from coproducts of biodiesel industry replacing soybean meal.
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Alves Júnior RT, Signoretti RD, Henriques LT, Silva EEPD, Bittencourt CA, de Oliveira JS, Pinheiro JK, and Gonzaga Neto S
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- Animals, Cattle physiology, Female, Diet veterinary, Digestion drug effects, Glycine max chemistry, Animal Nutritional Physiological Phenomena, Cottonseed Oil administration & dosage, Milk chemistry, Animal Feed analysis, Dietary Supplements analysis, Lactation, Biofuels analysis
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The objective of the present study was to evaluate the substitution of soybean meals by biodiesel co-products on the performance and nutrient balance of lactating cows on pasture. Twelve dairy cows (7/8 Holstein-Gir) in early lactation were included in this feeding trial. These animals had an average daily milk yield of 21.1 kg and were distributed in a 4 × 4 triple Latin square experimental design. Protein supplements fed to these lactating cows contained peanut meal, cotton and sunflower as a complete replacement for soybean meal. Nutrient intake and digestibility, milk production, microbial protein synthesis, energy and protein balance were the parameters assessed in this study. Supplements containing different biodiesel co-products did not influence (P > 0.05) the level of forage intake, supplementation and nutrient by lactating cows, except for the total digestible nutrients (TDN) intake which was higher (P ≤ 0.05) in cows supplemented with cottonseed meal. The cotton meal also promoted higher digestibility of TDN (P = 0.001) and total carbohydrates (TCHO; P = 0.001) of the diet and, as a result, higher energy consumption (P = 0.01) and lower negative energy balance (P = 0.01). Cottonseed meal is a co-product of the biodiesel industry that has the ability to replace soybean meal as a source of protein in the supplement without affecting performance and promoting greater energy availability, demonstrating its ability to reduce the effects of negative energy balance in cows at the beginning of lactation kept on tropical pastures., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
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- 2024
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19. Retrospective Analysis of Omicron in Minas Gerais, Brazil: Emergence, Dissemination, and Diversification.
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Fonseca PLC, Braga-Paz I, de Araújo E Santos LCG, Dias RC, de Souza CSA, Carvalho NO, Queiroz DC, Alves HJ, de Araújo JLF, Moreira FRR, Menezes MT, Menezes D, Silva ABPE, Ferreira JGG, Adelino TER, Bernardes AFL, Carobin NV, Carvalho RS, Ferrari CZ, Guimarães NR, Lamounier LO, Souza FG, Vargas LA, Ribeiro MO, Arruda MB, Alvarez P, Moreira RG, de Oliveira ES, Sabino AP, de Oliveira JS, Januário JN, Iani FCM, Souza RP, and Aguiar RS
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Brazil is one of the countries most affected by COVID-19, with the highest number of deaths recorded. Brazilian Health Institutions have reported four main peaks of positive COVID-19 cases. The last two waves were characterized by the emergence of the VOC Omicron and its sublineages. This study aimed to conduct a retrospective surveillance study illustrating the emergence, dissemination, and diversification of the VOC Omicron in 15 regional health units (RHUs) in MG, the second most populous state in Brazil, by combining epidemiological and genomic data. A total of 5643 confirmed positive COVID-19 samples were genotyped using the panels TaqMan SARS-CoV-2 Mutation and 4Plex SC2/VOC Bio-Manguinhos to define mutations classifying the BA.1, BA.2, BA.4, and BA.5 sublineages. While sublineages BA.1 and BA.2 were more prevalent during the third wave, BA.4 and BA.5 dominated the fourth wave in the state. Epidemiological and viral genome data suggest that age and vaccination with booster doses were the main factors related to clinical outcomes, reducing the number of deaths, irrespective of the Omicron sublineages. Complete genome sequencing of 253 positive samples confirmed the circulation of the BA.1, BA.2, BA.4, and BA.5 subvariants, and phylogenomic analysis demonstrated that the VOC Omicron was introduced through multiple international events, followed by transmission within the state of MG. In addition to the four subvariants, other lineages have been identified at low frequency, including BQ.1.1 and XAG. This integrative study reinforces that the evolution of Omicron sublineages was the most significant factor driving the highest peaks of positive COVID-19 cases without an increase in more severe cases, prevented by vaccination boosters.
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- 2024
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20. Cut-off points of adiposity indices associated with insulin resistance in Brazilian postpubertal adolescents.
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Sena BDS, de Andrade MIS, Oliveira JS, Leal VS, de Lira PIC, and de Arruda IKG
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Objectives: This study aims to establish cut-off points for lipid accumulation product and tri-ponderal mass index to identify insulin resistance (IR) in Brazilian postpubertal adolescents., Methods: We conducted an analysis of postpubertal adolescents enrolled in the national school-based cross-sectional study of cardiovascular risks in adolescents (ERICA-BRAZIL) from February 2013 to November 2014. IR was defined by homeostatic model assessment index for IR values ≥2.32 for girls and ≥2.87 for boys. The analysis involved calculating the area under receiver operating characteristic curves, sensitivity values, specificity, positive and negative predictive values, and positive and negative likelihood ratios to determine reference values of indices with optimal performance., Results: The sample was comprised of 14 026 adolescents, with 25.3% (95% confidence intervals: 24.6%-26.1%) exhibiting IR, more prevalent among girls and overweight individuals. The ideal lipid accumulation product cut-off points associated with IR were 13.5 for the total population, 13.8 for male adolescents, and 13.5 for girls. Regarding tri-ponderal mass index, the optimal cut-off values for identifying IR were 14.1, 13.9, and 14.5 kg/m³ in the general sample, boys, and girls, respectively., Conclusions: This study establishes cut-off points for adiposity indices, demonstrating their effectiveness in screening for IR in postpubertal Brazilian adolescents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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21. Effectiveness of a two-component nutritional strategy for blood pressure control in individuals with hypertension users of a public health system: a randomized controlled clinical trial.
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Bersch-Ferreira AC, Machado RHV, de Oliveira JS, Santos RHN, da Silva LR, Mota LGS, Pagano R, Sady ERR, Miyada DHK, Valeis N, Martins PN, de Almeida JC, Marchioni DML, Lara EMS, Gherardi-Donato ECS, Quinhoneiro D, Carvalho APPF, Machado MMA, Gedda LPF, Ferro CMB, Carvalho ACMS, Feoli AMP, Walker MS, Pinto SL, Silva KC, Bruscato NM, Moriguchi EH, Penafort AM, Santiago RA, Osório DRD, Rower HB, Barbosa MR, da Silva JGST, Weber B, Cavalcanti AB, and Marcadenti A
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- Humans, Male, Female, Middle Aged, Aged, Treatment Outcome, Adult, Public Health, Antihypertensive Agents therapeutic use, Hypertension diet therapy, Hypertension therapy, Blood Pressure
- Abstract
Objective: To evaluate the effectiveness of a nutritional strategy based on two components and adapted for the public health system on blood pressure, cardiometabolic features, self-care, qualify of life and diet quality in individuals with hypertension., Methods: NUPRESS was an open-label, parallel-group, superiority randomized controlled clinical trial in which participants at least 21 years with hypertension and poorly controlled blood pressure were randomly assigned (1 : 1 allocation ratio) to either an individualized dietary prescription according to nutritional guidelines (control group, n = 205); or a two-component nutrition strategy, including a goal-directed nutritional counseling and mindfulness techniques (NUPRESS [intervention] group, n = 205). Primary outcomes were SBP (mmHg) after 24 weeks of follow up and blood pressure control, defined as either having SBP more than 140 mmHg at baseline and achieving 140 mmHg or less after follow-up or having SBP 140 mmHg or less at baseline and reducing the frequency of antihypertensive drugs in use after follow-up., Results: In total, 410 participants were randomized and submitted to an intention-to-treat analysis regarding primary outcomes. Both groups decreased blood pressure, but after adjusting for baseline values, there was no significant difference between them on SBP [intervention-control difference: -0.03 (-3.01; 2.94); P = 0.98] nor blood pressure control [odds ratio 1.27 (0.82; 1.97); P = 0.28]. No differences between groups were also detected regarding secondary and tertiary outcomes., Conclusion: There was no difference between a two-component nutritional strategy and an established dietary intervention on blood pressure in participants with hypertension., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)
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- 2024
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22. Exercise prescriptions for patients on hemodialysis in Brazil: a scoping review.
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Ribeiro HS, Andrade FP, Leal DV, Oliveira JS, Wilund KR, Reboredo MM, and Viana JL
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- Humans, Brazil, Prescriptions statistics & numerical data, Exercise, Renal Dialysis, Exercise Therapy methods
- Abstract
Introduction: Exercise is being incorporated into the treatment of patients on hemodialysis; however, little is known about the major characteristics of these interventions., Objective: To describe the exercise protocols prescribed for hemodialysis patients in Brazil., Methods: A scoping review was conducted following JBI and Prisma-ScR guidelines. Searches were carried out in Medline, Embase and three other databases until May 2024. Other sources (websites, books and guidelines) were also investigated. Evidence from patients on hemodialysis describing exercise protocols in all settings and designs in Brazil was included., Results: Forty-five pieces of evidence were found, resulting in 54 exercise protocols from 16 Brazilian states. Strength exercises (33.3%), followed by aerobic exercises (22.2%), were the most prescribed, mainly to be performed during dialysis (85.2%). The most prevalent professionals supervising the programs were physiotherapists and exercise physiologists (37.0% and 18.5%, respectively). All protocols implemented the principles of type and frequency training, while progression was adopted in only 53.7%. The main prescription was three times per week (88.9%). Exercise intensity was predominantly determined by subjective methods (33.3%)., Conclusion: Aerobic and strength exercises during dialysis were the most commonly prescribed modalities in Brazil, with the majority of programs being properly supervised by qualified professionals. However, existing protocols have not employed systematic progression throughout the intervention, which would be appropriate for providing better physiological responses and adaptations.
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- 2024
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23. Bibliometric study and potential applications in the development of starch films with nanocellulose: A perspective from 2019 to 2023.
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Oliveira JP, Silva IBD, Costa JDSS, Oliveira JS, Oliveira EL, Coutinho ML, Almeida MEF, Landim LB, Silva NMCD, and Oliveira CP
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- Food Packaging methods, Nanoparticles chemistry, Tensile Strength, Nanofibers chemistry, Starch chemistry, Cellulose chemistry, Bibliometrics
- Abstract
This study aimed to perform a bibliometric analysis of starch films with nanocellulose, using the Scopus database and VOSviewer and Bibliometrix software. A total of 258 documents were identified between 2019 and 2023, reflecting a growing interest in research, particularly in journals such as the International Journal of Biological Macromolecules, Polymers, and Carbohydrate Polymers. The most common terms were "starch" (349 occurrences), "cellulose" (207), and "tensile strength" (175). China (58 articles), Brazil (38), and India (33) led scientific production, with authors like Ilyas (13 articles) and Sapuan (10) at the forefront. Approximately 41.7 % of the studies used corn starch. The analysis revealed that 66 % of the studies investigated films with cellulose nanofibrils (CNF), 32 % with cellulose nanocrystals (CNC), and 2 % with bacterial nanocellulose (CB). The majority of studies (94.1 %) used the casting method for film production. Additionally, 35.44 % focused on reinforcing films with nanocellulose, while 7 % developed blends with other biopolymers. About 59.44 % examined the performance of starch films for food packaging, 11.25 % explored practical applications in various foods. Furthermore, 7.94 % incorporated active agents to improve antioxidant and antimicrobial properties, 1.30 % investigated active packaging. Moreover, 2.36 % explored the use of films in materials engineering, and 2.36 % explored biomedical potential. Only 0.40 % evaluated the impact of films on wastewater treatment. The analysis highlights the potential of starch films with nanocellulose, demonstrating their diverse applications and the growing interest in the field., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
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- 2024
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24. Cutaneous Application of Capsaicin Cream Reduces Clinical Signs of Experimental Colitis and Repairs Intestinal Barrier Integrity by Modulating the Gut Microbiota and Tight Junction Proteins.
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Santos EA, Silva JL, Leocádio PCL, Andrade MER, Queiroz-Junior CM, Oliveira NSS, Alves JL, Oliveira JS, Aguilar EC, Boujour K, Cogliati B, Cardoso VN, Fernandes SOA, Faria AMC, and Alvarez-Leite JI
- Abstract
Capsaicin, a pungent compound in chili peppers, is described as having potent anti-inflammatory, antioxidant, and antimicrobial properties. It is also described as a potential modulator of the immune system and intestinal microbiota. Oral or rectal administration of capsaicin has been studied to treat or prevent colitis. However, those vias are often not well accepted due to the burning sensation that capsaicin can cause. Our objective was to evaluate whether the application of capsaicin skin creams (0.075%) would be effective in improving inflammation and epithelial barrier function as well as the composition of the gut microbiota in a model of mild colitis induced by dextran sulfate sodium (1.5%). The results showed that the cutaneous application of capsaicin reversed weight loss and decreased colon shortening and diarrhea, all typical signs of colitis. There was also an improvement in the intestinal epithelial barrier, preserving proteins from tight junctions. We also evaluated the biodistribution of
99m technetium-radiolabeled capsaicin (99m Tc-CAPS) applied to the back skin of the animals. We found significant concentrations of 99 mTc-Cap in the colon and small intestine after 2 and 4 h of administration. In addition, there was an increased expression of capsaicin receptor TRPV1 in the colon. Moreover, animals with colitis receiving cutaneous capsaicin presented a better short-chain fatty acid profile and increased levels of SIgA, suggesting increased microbiota diversity. In conclusion, our work opens avenues for further studies to better understand capsaicin's potential benefits and mechanisms in addressing colitis through cutaneous application., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)- Published
- 2024
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25. Lactobacillus delbrueckii CIDCA 133 fermented milk modulates inflammation and gut microbiota to alleviate acute colitis.
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de Jesus LCL, Freitas ADS, Dutra JDCF, Campos GM, Américo MF, Laguna JG, Dornelas EG, Carvalho RDO, Vital KD, Fernandes SOA, Cardoso VN, de Oliveira JS, de Oliveira MFA, Faria AMC, Ferreira E, Souza RO, Martins FS, Barroso FAL, and Azevedo V
- Subjects
- Animals, Mice, Probiotics therapeutic use, Male, Mice, Inbred C57BL, Disease Models, Animal, Intestinal Mucosa microbiology, Intestinal Mucosa metabolism, Inflammation, Colon microbiology, Colon metabolism, Lactobacillus, Gastrointestinal Microbiome drug effects, Colitis microbiology, Colitis chemically induced, Colitis metabolism, Colitis drug therapy, Lactobacillus delbrueckii metabolism, Cultured Milk Products microbiology, Dextran Sulfate
- Abstract
Lactobacillus delbrueckii subsp. lactis CIDCA 133 is a health-promoting bacterium that can alleviate gut inflammation and improve the epithelial barrier in a mouse model of mucositis. Despite these beneficial effects, the protective potential of this strain in other inflammation models, such as inflammatory bowel disease, remains unexplored. Herein, we examined for the first time the efficacy of Lactobacillus delbrueckii CIDCA 133 incorporated into a fermented milk formulation in the recovery of inflammation, epithelial damage, and restoration of gut microbiota in mice with dextran sulfate sodium-induced colitis. Oral administration of Lactobacillus delbrueckii CIDCA 133 fermented milk relieved colitis by decreasing levels of inflammatory factors (myeloperoxidase, N-acetyl-β-D-glucosaminidase, toll-like receptor 2, nuclear factor-κB, interleukins 10 and 6, and tumor necrosis factor), secretory immunoglobulin A levels, and intestinal paracellular permeability. This immunobiotic also modulated the expression of tight junction proteins (zonulin and occludin) and the activation of short-chain fatty acids-related receptors (G-protein coupled receptors 43 and 109A). Colonic protection was effectively associated with acetate production and restoration of gut microbiota composition. Treatment with Lactobacillus delbrueckii CIDCA 133 fermented milk increased the abundance of Firmicutes members (Lactobacillus genus) while decreasing the abundance of Proteobacteria (Helicobacter genus) and Bacteroidetes members (Bacteroides genus). These promising outcomes influenced the mice's mucosal healing, colon length, body weight, and disease activity index, demonstrating that this immunobiotic could be explored as an alternative approach for managing inflammatory bowel disease., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
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- 2024
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26. Prospective, randomized, controlled trial assessing the effects of a driving pressure-limiting strategy for patients with acute respiratory distress syndrome due to community-acquired pneumonia (STAMINA trial): protocol and statistical analysis plan.
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Maia IS, Medrado FA Jr, Tramujas L, Tomazini BM, Oliveira JS, Sady ERR, Barbante LG, Nicola ML, Gurgel RM, Damiani LP, Negrelli KL, Miranda TA, Santucci E, Valeis N, Laranjeira LN, Westphal GA, Fernandes RP, Zandonai CL, Pincelli MP, Figueiredo RC, Bustamante CLS, Norbin LF, Boschi E, Lessa R, Romano MP, Miura MC, Alencar Filho MS, Dantas VCS, Barreto PA, Hernandes ME, Grion CMC, Laranjeira AS, Mezzaroba AL, Bahl M, Starke AC, Biondi RS, Dal-Pizzol F, Caser EB, Thompson MM, Padial AA, Veiga VC, Leite RT, Araújo G, Guimarães M, Martins PA, Lacerda FH, Hoffmann Filho CR, Melro L, Pacheco E, Ospina-Táscon GA, Ferreira JC, Freires FJC, Machado FR, Cavalcanti AB, and Zampieri FG
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- Humans, Brazil epidemiology, Colombia epidemiology, Intensive Care Units, Pneumonia therapy, Prospective Studies, Tidal Volume, Randomized Controlled Trials as Topic, Multicenter Studies as Topic, Community-Acquired Infections therapy, Positive-Pressure Respiration methods, Respiratory Distress Syndrome therapy, Respiratory Distress Syndrome physiopathology
- Abstract
Background: Driving pressure has been suggested to be the main driver of ventilator-induced lung injury and mortality in observational studies of acute respiratory distress syndrome. Whether a driving pressure-limiting strategy can improve clinical outcomes is unclear., Objective: To describe the protocol and statistical analysis plan that will be used to test whether a driving pressure-limiting strategy including positive end-expiratory pressure titration according to the best respiratory compliance and reduction in tidal volume is superior to a standard strategy involving the use of the ARDSNet low-positive end-expiratory pressure table in terms of increasing the number of ventilator-free days in patients with acute respiratory distress syndrome due to community-acquired pneumonia., Methods: The ventilator STrAtegy for coMmunIty acquired pNeumoniA (STAMINA) study is a randomized, multicenter, open-label trial that compares a driving pressure-limiting strategy to the ARDSnet low-positive end-expiratory pressure table in patients with moderate-to-severe acute respiratory distress syndrome due to community-acquired pneumonia admitted to intensive care units. We expect to recruit 500 patients from 20 Brazilian and 2 Colombian intensive care units. They will be randomized to a driving pressure-limiting strategy group or to a standard strategy using the ARDSNet low-positive end-expiratory pressure table. In the driving pressure-limiting strategy group, positive end-expiratory pressure will be titrated according to the best respiratory system compliance., Outcomes: The primary outcome is the number of ventilator-free days within 28 days. The secondary outcomes are in-hospital and intensive care unit mortality and the need for rescue therapies such as extracorporeal life support, recruitment maneuvers and inhaled nitric oxide., Conclusion: STAMINA is designed to provide evidence on whether a driving pressure-limiting strategy is superior to the ARDSNet low-positive end-expiratory pressure table strategy for increasing the number of ventilator-free days within 28 days in patients with moderate-to-severe acute respiratory distress syndrome. Here, we describe the rationale, design and status of the trial.
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- 2024
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27. Efficacy and safety of adenosine, rapid ventricular pacing and hypothermia in cerebral aneurysms clipping: a systematic review and meta-analysis.
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Nager GB, Pontes JPM, Udoma-Udofa OC, Gomes FC, Larcipretti ALL, de Oliveira JS, Dagostin CS, Fernandes MNF, and de Andrade Bannach M
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- Humans, Hypothermia, Induced methods, Treatment Outcome, Neurosurgical Procedures methods, Cardiac Pacing, Artificial methods, Intracranial Aneurysm surgery, Adenosine therapeutic use
- Abstract
Background and Objectives: Cerebral aneurysms in complex anatomical locations and intraoperative rupture can be challenging. Many methods to reduce blood flow can facilitate its exclusion from the circulation. This study evaluated the safety and efficacy of using adenosine, rapid ventricular pacing, and hypothermia in cerebral aneurysm clipping., Methods: Databases (PubMed, Embase, and Web of Science) were systematically searched for studies documenting the use of adenosine, rapid ventricular pacing, and hypothermia in cerebral aneurysm clipping and were included in this single-arm meta-analysis. The primary outcome was 30-day mortality. Secondary outcomes included neurological outcomes by mRs and GOS, and cardiac outcomes. We evaluated the risk of bias using ROBIN-I, a tool developed by the Cochrane Collaboration. OpenMetaAnalyst version 2.0 was used for statistical analysis and I2 measured data heterogeneity. Heterogeneity was defined as an I
2 > 50%., Results: Our systematic search yielded 10,100 results. After the removal of duplicates and exclusion by title and abstract, 64 studies were considered for full review, of which 29 were included. The overall risk of bias was moderate. The pooled proportions of the adenosine analysis for the different outcomes were: For the primary outcome: 11,9%; for perioperative arrhythmia: 0,19%; for postoperative arrhythmia: 0,56%; for myocardial infarction incidence: 0,01%; for follow-up good recovery (mRs 0-2): 88%; and for neurological deficit:14.1%. In the rapid ventricular pacing analysis, incidences were as follows: peri operative arrhythmia: 0,64%; postoperative arrhythmia: 0,3%; myocardial infarction: 0%. In the hypothermia analysis, the pooled proportion of 30-day mortality was 11,6%. The incidence of post-op neurological deficits was 35,4% and good recovery under neurological analysis by GOS was present in 69.2%., Conclusion: The use of the three methods is safe and the related complications were very low. Further studies are necessary, especially with comparative analysis, for extended knowledge., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2024
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28. A Mediterranean diet improves glycation markers in healthy people and in those with chronic diseases: a systematic review of clinical trials.
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Oliveira JS, da Silva JA, de Freitas BVM, Alfenas RCG, and Bressan J
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Context: Consumption of the Mediterranean diet (MedDiet) has beneficial effects on cardiometabolic health and aging., Objective: This systematic review aimed to critically investigate the effect of the MedDiet on glycation markers in healthy or overweight individuals with type 2 diabetes or cardiovascular disease., Data Sources: MEDLINE, EMBASE, Web of Science, and the Cochrane Library were searched, using the terms "Mediterranean diet" AND "glycation end products, advanced"., Data Extraction: Three randomized and 3 nonrandomized clinical trials, containing data on 2935 adult and elderly individuals with normal weight or overweight, were included. All extracted data were compiled, compared, and critically analyzed., Data Analysis: The authors of most of the studies demonstrated a reduction in serum concentrations of advanced glycation end products (AGEs), such as εN-carboxymethyllysine and methylglyoxal, and in skin autofluorescence levels after at least 4 weeks of adherence to the MedDiet. The MedDiet also led to positive effects on gene expression of receptors for AGEs, as RAGE and AGER1, and an enzyme involved in detoxification (glyoxalase I). There is no evidence that short-term adherence affects glycation markers., Conclusions: Glycation markers improved in response to the MedDiet. The possible mechanisms involved may be related to the low AGE and refined sugars content of the diet, as well as its high monounsaturated fatty acid, phenolic compound, and dietary fiber contents., Systematic Review Registration: PROSPERO registration no. CRD42021284006., (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Life Sciences Institute. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
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- 2024
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29. Purification and partial physical-chemical characterization of a new bovine trypsin proteoform (zeta-trypsin).
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Cruz FT, Rosa DP, Vasconcelos AVB, de Oliveira JS, Bleicher L, and Santos AMC
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- Animals, Cattle, Kinetics, Enzyme Stability, Protein Conformation, Trypsin chemistry, Trypsin metabolism
- Abstract
Recent advancements in enzyme research have unveiled a new proteoform of bovine trypsin, expanding our understanding of this well-characterized enzyme. While generally similar to other trypsins, this novel proteoform comprises three polypeptide chains, marking a significant difference in activity, kinetic properties, and conformational stability. Compared with the already known bovine trypsin proteoforms, the results showed a lower: activity, k
cat and kcat .KM -1 and protein 'foldedness' ratio for the new proteoform. Molecular autolysis, a common feature in trypsin and chymotrypsin, has been explored through comparative physical chemistry properties with other proteoforms. This new proteoform of trypsin not only enriches the existing enzyme repertoire but also promises to shed light on the intricate physiological pathway for enzyme inactivation. Our results suggest that the new trypsin proteoform is one of the likely final pathways for enzyme inactivation in a physiological environment. This discovery opens up new avenues for further research into the functional implications of this new trypsin proteoform., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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30. Unveiling the anticancer potential of the ethanolic extract from Trichoderma asperelloides .
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Oliveira ACR, De Oliveira FS, Bráz AF, Oliveira JS, Lima-Santos J, and Dias AAM
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The discovery of new therapeutic alternatives for cancer treatment is essential for improving efficacy and specificity, overcoming resistance, and enabling a more personalized approach for each patient. We investigated the antitumor activity of the crude ethanolic extract of the fungus Trichoderma asperelloides (ExtTa) and its interaction with chemotherapeutic drugs. It was observed, by MTT cytotoxicity assay, that ExtTa significantly reduced cell viability in breast adenocarcinoma, glioblastoma, lung carcinoma, melanoma, colorectal carcinoma, and sarcomas cell lines. The highest efficacy and selectivity of ExtTa were found against glioblastoma T98G and colorectal HCT116 cell lines. ExtTa is approximately four times more cytotoxic to those tumor cells than to non-cancer cell lines. A synergistic effect between ExtTa and doxorubicin was found in the treatment of osteosarcoma Saos-2 cells, as well as with 5-fluorouracil in the treatment of HCT116 colorectal carcinoma cells using CompuSyn software. Our data unravel the presence of bioactive compounds with cytotoxic effects against cancer cells present in T. asperelloides ethanolic crude extract, with the potential for developing novel anticancer agents., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Oliveira, De Oliveira, Bráz, Oliveira, Lima-Santos and Dias.)
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- 2024
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31. Social inequities in food deserts and food swamps in a northeastern Brazilian capital.
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Lima da Silva JM, Oliveira JS, Casagrande Borges D, Honório OS, Mendes LL, and Canuto R
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- Humans, Brazil, Food Supply, Socioeconomic Factors, Residence Characteristics, Food, Food Deserts, Wetlands
- Abstract
This study identified food deserts and swamps, investigating their associations with socioeconomic and demographic conditions. This ecological study was conducted using data from urban census tracts in the city of Recife, which were considered the unit of analysis. Information on food retail was obtained from government sources in 2019. Census tracts below the 25th percentile in the density of healthy food retail (i.e., those that predominantly sell natural or minimally processed foods, mixed businesses, and super- and hypermarkets) were classified as food deserts. Census tracts above the 25th percentile in the density of unhealthy food retail (i.e., those selling primarily ultra-processed foods) were considered food swamps. The socioeconomic and demographic conditions of the census tracts were evaluated using variables from the 2010 census (per capita income, average income, race, literacy of the head of household, and the availability of essential services) and the Health Vulnerability Index. Census tracts considered food deserts (28.5%) were more vulnerable, characterized by lower income and access to essential services, more illiterate residents and more minorities (Black/Indigenous/mixed race). Food swamps (73.47%) were more prevalent in less vulnerable neighbourhoods characterized by higher percentages of literate residents and Whites, greater purchasing power, and better basic sanitation. The characteristics of Recife's food deserts and swamps demonstrate social inequalities in the food environment. Public facilities could play a vital role in promoting healthy eating within food deserts. Additionally, future implementation of taxes on ultra-processed foods and the provision of tax subsidies to natural or minimally processed food sellers might contribute to fostering healthier dietary choices.
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- 2024
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32. Consumption of in natura and ultra-processed foods in adults: an analysis of social, metabolic, and lifestyle determinants.
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Oliveira RKG, Domingos Júnior IR, Leal VS, Oliveira JS, Lira PIC, and Souza NP
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- Adolescent, Adult, Aged, Female, Humans, Male, Middle Aged, Young Adult, Alcohol Drinking epidemiology, Body Mass Index, Brazil, Cross-Sectional Studies, Diet statistics & numerical data, Feeding Behavior, Fruit, Sociodemographic Factors, Vegetables, Food, Processed, Life Style, Socioeconomic Factors
- Abstract
Objective: To assess the social, metabolic, and lifestyle determinants of consumption of fruits, vegetables, and greens (FVG) and ultra-processed food (ULT) in adults from Pernambuco., Methods: Cross-sectional and analytical study, conducted in 2015/2016. In addition to sociodemographic variables, the determinants of lifestyle were level of physical activity, alcohol consumption, tobacco use, and metabolic variables were self-reported hypertension, blood glucose, and Body Mass Index (BMI). Consumption was measured by the Food Frequency Questionnaire, then created the Frequency of Consumption Index (SFI) of the mean intake of ULT and FVG foods. The indices of FVG and ULT consumption were transformed into quartiles and these variables were included in the multinomial logistic regression, considering their determinants when p<0.05., Results: The sample was representative of the state, with 1,067 people being interviewed, whose intake of ULT was higher than that of FVG in the lowest and highest quartile of the consumption index. Consumption of fruit and vegetables was higher in higher consumption of alcoholic beverages (p=0.031) and BMI>25 kg/m2 (p=0.047); and lower in the lowest income (p=0.001). ULT intake was higher in young adults (p=0.005), lower income (p=0.044), and controlled blood glucose (p=0.021). Rural areas were 52% less exposed to medium-high ULT consumption (p<0.006)., Conclusion: Higher rate of ULT consumption in relation to fresh foods, with income as a common determinant, inversely associated with ULT intake and directly related to FVG, which demands structuring policies.
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- 2024
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33. Is time really brain in stroke therapy?: A meta-analysis of mechanical thrombectomy up to 155 h post ictus.
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Larcipretti ALL, Gomes FC, Dagostin CS, Nager GB, Udoma-Udofa OC, Pontes JPM, de Oliveira JS, and Bannach MA
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- Humans, Treatment Outcome, Time Factors, Time-to-Treatment statistics & numerical data, Stroke surgery, Stroke therapy, Mechanical Thrombolysis methods, Thrombectomy methods, Ischemic Stroke surgery, Ischemic Stroke therapy
- Abstract
Background and Objectives: Mechanical thrombectomy (MT) has been established as the gold standard of treatment for patients with Acute Ischemic Stroke (AIS) who present up to 6 h after the onset of the stroke. Recently, the DEFUSE-3 and DAWN trials established the safety of starting the MT procedure up to 16 and 24 h after the patient was last seen well, respectively. The purpose of this study is to assess the safety and functional effects of thrombectomy in individuals with AIS detected at a late stage (> 24 h)., Materials and Methods: PubMed, Web of Science, Embase, and Cochrane databases were thoroughly searched for research on MT in patients in the extremely late time window after AIS. The primary outcomes were symptomatic cerebral hemorrhage, 90-day mortality, Thrombolysis in Cerebral Infarction (TICI) 2b-3, and Modified Rankin Scale (mRS) 0-2., Results: Our study included fifteen studies involving a total of 1,221 patients who presented with AIS and an extended time window. The primary outcome of interest was the favorable functional outcome, mRS 0-2 at 90 days. The pooled proportion for this outcome was 45% (95% confidence interval 34-58%). Other outcomes included the TICI 2b or 3 (successful recanalization), which was reported in 12 studies and had a 79% incidence in the study population (95% CI 68-87%). Complications included: symptomatic intracranial hemorrhage (sICH), which revealed an incidence of 7% in the study population (95% CI 5-10%); and 90-day mortality, which reported a 27% incidence (95% CI 24-31%). In addition, we conducted a comparative analysis between endovascular treatment and standard medical therapy., Conclusion: Our meta-analysis provides evidence that supports the need of further randomized and prospective clinical trials to better assess the effectiveness and safety of MT in these patients., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
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- 2024
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34. Evaluation of internal markers in digestibility estimation for sheep and goats.
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da Silva MA, de Oliveira JS, Santos EM, de Lima Cruz GF, de Araújo GGL, da Silva MMC, de Carvalho Sobral G, de Farias Ramos JP, Gomes PGB, Leite GM, Coutinho Santos MA, da Cunha Torres Júnior P, and Matias Filho AF
- Subjects
- Animals, Sheep, Nutrients, Dietary Supplements, Nitrogen, Goats, Detergents
- Abstract
The objective of this study was to compare the indirect methods of obtaining digestibility with the direct method of total fecal collection to estimate the apparent digestibility of nutrients in sheep and goats supplemented with non-protein nitrogen. Five goats and five sheep with no defined racial pattern were used, distributed in two 5 × 5 Latin squares, with split plots, considering the diets as plots and the apparent digestibility determination methodologies as subplots. The diets were composed of buffelgrass hay and the addition, via ruminal infusion, of increasing amounts of nitrogen supplementation in order to gradually raise the CP level of the basal diet in intervals of 2% points, that is, + 2, +4, + 6 and + 8%. Samples of the feeds offered, and the leftovers were collected daily during the five days of collection to determine the nutrient intake, as well as the total collection of feces to determine the apparent digestibility of the nutrients. The amount of fecal dry matter excreted was estimated by the concentration of Indigestible Acid Detergent Fiber (ADFi), Indigestible Neutral Detergent Fiber (NDFi), Indigestible Dry Matter at 244 h (DMi 244 h) and Indigestible Dry Matter at 264 h (DMi 264 h). Among the evaluated markers, DMi 264 h had the lowest accuracy in estimating fecal excretion and nutrient digestibility. For the goat species, the markers ADFi and DMi 244 h proved to be able to adequately predict fecal excretion and digestibility indices, while NDFi stood out for both species. Among the evaluated markers, NDFi is the one that most accurately estimates the nutrient digestibility of the diet for goats and sheep., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
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- 2024
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35. Association of vitamin D and cognition in people with type 2 diabetes: a systematic review.
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da Silva AD, Oliveira JS, de Castro IC, Paiva WC, Gomes JMG, and Pimenta LCJP
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- Adult, Aged, Humans, Cognition, Dietary Supplements, Vitamin D, Vitamins, Observational Studies as Topic, Randomized Controlled Trials as Topic, Young Adult, Middle Aged, Diabetes Mellitus, Type 2 complications, Vitamin D Deficiency complications, Vitamin D Deficiency epidemiology
- Abstract
Context: There is a high prevalence of vitamin D deficiency and impaired cognitive function in people with type 2 diabetes mellitus (T2DM)., Objective: To critically and systematically review the literature on the association between vitamin D status and cognitive performance in people with type 2 diabetes., Data Sources: This review was conducted according to PRISMA recommendations. MEDLINE, SCOPUS, the Cochrane Library, and Web of Science databases were searched using the terms "Diabetes Mellitus, Type 2", "Cognitive Function", and "Vitamin D"., Data Extraction: Eight observational and 1 randomized study were included, containing data of 14 648 adult and elderly individuals (19-74 y). All extracted data were compiled, compared, and critically analyzed., Data Analysis: There is no strong evidence that lower serum concentrations of vitamin D and vitamin D-binding protein are associated with worsening cognitive function in individuals with T2DM. Vitamin D supplementation (12 wk) improved the scores of some executive functioning tests, although there was no difference between low doses (5000 IU/wk) and high doses (50 000 IU/wk)., Conclusions: There is no high-quality evidence demonstrating an association between vitamin D status and cognitive function, or clinical benefits on cognition from vitamin D supplementation in individuals with T2DM. Future studies are needed. Systematic Review Registration: PROSPERO registration no. CRD42021261520., (© The Author(s) 2023. Published by Oxford University Press on behalf of the International Life Sciences Institute. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
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- 2024
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36. Differences in Falls and Physical Activity in Older Women From Two Generations.
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Kwok WS, Khalatbari-Soltani S, Dolja-Gore X, Byles J, Oliveira JS, Pinheiro MB, and Sherrington C
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- Humans, Female, Aged, Longitudinal Studies, Australia epidemiology, Risk Factors, Exercise, Women's Health
- Abstract
Background: Falls and physical inactivity increase with age. However, physical activity, falls and their associations in older people born at different times are unclear., Methods: Women born 1921-26 and 1946-51 who completed follow-up questionnaires in 1999 (n = 8 403, mean (SD) age: 75 (1) years) and 2019 (n = 7 555; 71 (1) years) in the Australian Longitudinal Study on Women's Health. Self-reported noninjurious and injurious falls in the previous 12 months and weekly amounts and types of physical activity (brisk walking, moderate- and vigorous-intensity) were compared between the cohorts using Chi-square tests. Associations between physical activity, and noninjurious and injurious falls were estimated using multinomial logistic regressions informed by a directed acyclic graph., Results: A greater proportion of the later (1946-51) cohort (59%) reached 150-300 minutes of weekly physical activity, as recommended by the World Health Organization, compared to the earlier (1921-26) cohort (43%, p < .001). A greater proportion of the later cohort reported noninjurious falls (14% vs 8%). Both cohorts reported similar proportions of injurious falls (1946-51:15%, 1921-26:14%). In both cohorts, participation in 150-300 minutes of physical activity was associated with lower odds of noninjurious falls (adjusted Odds Ratio, 95% CI: 1921-26: 0.66, 0.52-0.84; 1946-51: 0.78, 0.63-0.97) and injurious falls (1921-26: 0.72, 0.60-0.87; 1946-51: 0.78, 0.64-0.96)., Conclusions: Participation in recommended levels of physical activity was associated with reduced falls in both cohorts. However, generational differences were found with more falls and more physical activities in the women born later. Future studies could examine the reasons contributing to the generational differences., (© The Author(s) 2024. Published by Oxford University Press on behalf of The Gerontological Society of America.)
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- 2024
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37. Responsiveness of Patient-Reported and Device-Based Physical Activity Measures: Secondary Analysis of Four Randomized Trials.
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Baldwin JN, He J, Oliveira JS, Bates A, Tiedemann A, Hassett L, Sherrington C, and Pinheiro MB
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- Aged, Humans, Exercise Therapy, Randomized Controlled Trials as Topic, Walking, Middle Aged, Exercise, Resistance Training
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Purpose: This study aimed to compare the responsiveness of patient-reported and device-based instruments within four physical activity trials., Methods: This was a secondary analysis of four randomized trials that used both a patient-reported outcome measure (the Incidental and Planned Exercise Questionnaire (IPEQ)) and a device-based instrument (ActiGraph or ActivPAL) to measure physical activity. The four trials included were (i) Activity and MObility UsiNg Technology (AMOUNT), digitally enabled exercises in those undertaking aged care and neurological rehabilitation; (ii) Balance Exercise Strength Training at Home, home-based balance and strength exercises in community-dwelling people 65 yr or older; (iii) Coaching for Healthy Ageing (CHAnGE), physical activity coaching and fall prevention intervention in community-dwelling people 60 yr or older; and (iv) Fitbit trial, fall prevention and physical activity promotion with health coaching and activity monitor in community-dwelling people 60 yr or older. We estimated treatment effects for all variables within each physical activity instrument using regression analyses and expressed results as effect sizes (ES)., Results: Overall, device-based instruments were more responsive among healthy older adults (ES range, 0.01 to 0.32), whereas the IPEQ was more responsive among adults requiring rehabilitation (ES range, -0.06 to 0.35). Both the IPEQ and device-based instruments were more responsive in trials that promoted walking via coaching participants to increase their daily steps (AMOUNT (ES range, -0.06 to 0.35), CHAnGE (ES range, -0.24 to 0.22), and Fitbit trial (ES range, -0.23 to 0.32)). Individual variables within the IPEQ and device-based instruments varied in their responsiveness (ES range, -0.13 to 0.20)., Conclusions: Both the IPEQ and device-based instruments are able to detect small changes in physical activity levels. However, responsiveness varies across different interventions and populations. Our findings provide guidance for researchers and clinicians in selecting an appropriate instrument to measure changes in physical activity., (Copyright © 2023 by the American College of Sports Medicine.)
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- 2024
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38. Exercise Interventions Delivered Through Telehealth to Improve Physical Functioning for Older Adults with Frailty, Cognitive, or Mobility Disability: A Systematic Review and Meta-Analysis.
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Dawson R, Oliveira JS, Kwok WS, Bratland M, Rajendran IM, Srinivasan A, Chu CY, Pinheiro MB, Hassett L, and Sherrington C
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- Humans, Aged, Frailty therapy, Female, Male, Middle Aged, Aged, 80 and over, Accidental Falls prevention & control, Postural Balance physiology, Exercise Therapy methods, Quality of Life, Telemedicine, Mobility Limitation
- Abstract
Introductions: This study assessed the effects of telehealth-delivered exercise interventions on physical functioning for older adults and explored implementation measures related to program delivery. Methods: We conducted a systematic review of studies investigating effects of exercise interventions delivered through telehealth in adults 60+ years of age with frailty, mobility, or cognitive disability on mobility, strength, balance, falls, and quality of life (QoL). Electronic databases (MEDLINE, CINAHL, SPORTSDiscus, and Physiotherapy Evidence Database) were searched from inception until May 2022. Evidence certainty was assessed with Grading of Recommendations, Assessment, Development, and Evaluation and meta-analysis summarized study effects. Results: A total of 11 studies were included, 5 randomized controlled trials, 2 pilot studies, and 4 feasibility studies. The overall certainty of evidence was rated as "low" or "very low." Pooled between-group differences were not statistically significant, but effect sizes suggested that telehealth produced a moderate improvement on mobility ( n = 5 studies; standardized mean difference [SMD] = 0.63; 95% confidence interval [CI] = -0.25 to 1.51; p = 0.000, I
2 = 86%) and strength ( n = 4; SMD = 0.73; 95% CI = -0.10 to 1.56; p = 0.000, I2 = 84%), a small improvement on balance ( n = 3; SMD = 0.40; 95% CI = -035 to 1.15; p = 0.012, I2 = 78%), and no effect on QoL. Analysis of implementation measures suggested telehealth to be feasible in this population, given high rates of acceptability and adherence with minimal safety concerns. Discussion: Telehealth may provide small to moderate benefits on a range of physical outcomes and appears to be well received in aged care populations.- Published
- 2024
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39. Prevalence of sarcopenia in patients with chronic kidney disease: a global systematic review and meta-analysis.
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Duarte MP, Almeida LS, Neri SGR, Oliveira JS, Wilkinson TJ, Ribeiro HS, and Lima RM
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- Humans, Prevalence, Sarcopenia epidemiology, Renal Insufficiency, Chronic epidemiology, Renal Insufficiency, Chronic complications
- Abstract
Sarcopenia is a risk factor for adverse clinical outcomes in chronic kidney disease (CKD) patients, including mortality. Diagnosis depends on adopted consensus definition and cutoff values; thus, prevalence rates are generally heterogeneous. We conducted a systematic review and meta-analysis to investigate the global prevalence of sarcopenia and its traits across the wide spectrum of CKD. A systematic search was conducted using databases, including MEDLINE and EMBASE, for observational studies reporting the prevalence of sarcopenia. We considered sarcopenia according to the consensus definition of the European Working Group on Sarcopenia in Older People (EWGSOP), the Asian Working Group for Sarcopenia, the Foundation for the National Institutes of Health Sarcopenia Project, and the International Working Group on Sarcopenia (IWGS). Subgroup analyses by CKD stages, consensus, and gender were performed. Pooled prevalence was obtained from random-effect models. A total of 140 studies (42 041 patients) across 25 countries were included in this systematic review and meta-analyses. Global prevalence of sarcopenia was 24.5% [95% confidence interval (CI): 20.9-28.3) and did not differ among stages (P = 0.33). Prevalence varied according to the consensus definition from 11% to 30%, with no significant difference (P = 0.42). Prevalence of severe sarcopenia was 21.0% (95% CI: 11.7-32.0), with higher rates for patients on dialysis (26.2%, 95% CI: 16.6-37.1) compared to non-dialysis (3.0%, 95% CI: 0-11.1; P < 0.01). Sarcopenic obesity was observed in 10.8% (95% CI: 3.5-21.2). Regarding sarcopenia traits, low muscle strength was found in 43.4% (95%CI: 35.0-51.9), low muscle mass in 29.1% (95% CI: 23.9-34.5), and low physical performance in 38.6 (95% CI: 30.9-46.6) for overall CKD. Prevalence was only higher in patients on dialysis (50.0%, 95% CI: 41.7-57.4) compared to non-dialysis (19.6%, 95% CI: 12.8-27.3; P < 0.01) for low muscle strength. We found a high global prevalence of sarcopenia in the wide spectrum of CKD. Low muscle strength, the primary sarcopenia trait, was found in almost half of the overall population with CKD. Patients on dialysis were more prevalent to low muscle strength and severe sarcopenia. Nephrology professionals should be aware of regularly assessing sarcopenia and its traits in patients with CKD, especially those on dialysis., (© 2024 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by Wiley Periodicals LLC.)
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- 2024
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40. BENEFITS OF A CLINICAL PATHWAY IN TOTAL KNEE ARTHROPLASTY.
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Ferreira MC, Silva G, Marinelli CPA, de Oliveira JS, Mathiasi PA, and Camanho GL
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Objective: Demonstrate whether a multiprofessional Clinical Pathway Program in Total Knee Arthroplasty (CPPA) contributesto optimizing hospital care., Method: Retrospective study of medical data of care indicators in 310 patients divided into two groups: A- who underwent arthroplasty in the last biennium before the introduction of the CPPA (n=144) and group B- who underwent TKA in the biennium after the introduction of the CPPA (n=166)., Results: Postoperative showed a significant difference in favor of group B over group A for hospitalization time in days 4.33 ± 2.79 and 5.4 ± 1.67 (p<0.001), time of prophylactic antibiotic in hours 28.13 ± 33.77 and 81.49 ± 40.91 (p<0.001), referral to the intensive care unit 40.9% and 73.4% (p<0.001), initiation of thromboprophylaxis within 24 hours 97.9% and 82.5% (p<0.001), use of elastic stockings and/or intermittent compression prescribed for thromboprophylaxis 89.5% and 31.2% (p<0.001), initiation of rehabilitation within 24 hours 90.1% and 66.1% (p<0.001), readmissions within 30 days 4.1% and 3% (p = 0.76), readmissions 90 days 2.7% and 6.6% (p = 0.183), transfusions 5.5% and 15.2% (p = 0.033)., Conclusion: The implementation of a multiprofessional CPPA contributed to the implementation of care protocols, favoring greater patient safety. Level of Evidence III; Retrospective Comparative Study. , Competing Interests: All authors declare no potential conflict of interest related to this article.
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- 2024
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41. The Cloning and Characterization of a Three-Finger Toxin Homolog (NXH8) from the Coralsnake Micrurus corallinus That Interacts with Skeletal Muscle Nicotinic Acetylcholine Receptors.
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Roman-Ramos H, Prieto-da-Silva ÁRB, Dellê H, Floriano RS, Dias L, Hyslop S, Schezaro-Ramos R, Servent D, Mourier G, de Oliveira JL, Lemes DE, Costa-Lotufo LV, Oliveira JS, Menezes MC, Markus RP, and Ho PL
- Subjects
- Animals, Amino Acid Sequence, Male, Elapid Venoms chemistry, Elapid Venoms toxicity, Elapid Venoms genetics, Receptors, Nicotinic metabolism, Receptors, Nicotinic genetics, Muscle, Skeletal metabolism, Muscle, Skeletal drug effects, Coral Snakes, Cloning, Molecular
- Abstract
Coralsnakes ( Micrurus spp.) are the only elapids found throughout the Americas. They are recognized for their highly neurotoxic venom, which is comprised of a wide variety of toxins, including the stable, low-mass toxins known as three-finger toxins (3FTx). Due to difficulties in venom extraction and availability, research on coralsnake venoms is still very limited when compared to that of other Elapidae snakes like cobras, kraits, and mambas. In this study, two previously described 3FTx from the venom of M. corallinus , NXH1 (3SOC1_MICCO), and NXH8 (3NO48_MICCO) were characterized. Using in silico, in vitro, and ex vivo experiments, the biological activities of these toxins were predicted and evaluated. The results showed that only NXH8 was capable of binding to skeletal muscle cells and modulating the activity of nAChRs in nerve-diaphragm preparations. These effects were antagonized by anti-rNXH8 or antielapidic sera. Sequence analysis revealed that the NXH1 toxin possesses eight cysteine residues and four disulfide bonds, while the NXH8 toxin has a primary structure similar to that of non-conventional 3FTx, with an additional disulfide bond on the first loop. These findings add more information related to the structural diversity present within the 3FTx class, while expanding our understanding of the mechanisms of the toxicity of this coralsnake venom and opening new perspectives for developing more effective therapeutic interventions.
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- 2024
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42. Effect of a coaching intervention to enhance physical activity and prevent falls in community-dwelling people aged 60+ years: a cluster randomised controlled trial.
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Oliveira JS, Sherrington C, Rissel C, Howard K, Tong A, Merom D, Wickham J, Bauman AE, Lord SR, Lindley RI, Simpson JM, Allman-Farinelli M, Kirkham C, Ramsay E, O'Rourke S, and Tiedemann A
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- Humans, Female, Aged, Male, Quality of Life, Fear, Exercise, Independent Living, Mentoring
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Objectives: To evaluate the effect of a coaching intervention compared with control on physical activity and falls rate at 12 months in community-dwelling people aged 60+ years., Design: Cluster randomised controlled trial., Setting: Community-dwelling older people., Participants: 72 clusters (605 participants): 37 clusters (290 participants) randomised to the intervention and 35 (315 participants) to control., Intervention: Intervention group received written information, fall risk assessment and prevention advice by a physiotherapist, activity tracker and telephone-based coaching from a physiotherapist focused on safe physical activity. Control group received written information and telephone-based dietary coaching. Both groups received up to 19 sessions of telephone coaching over 12 months., Outcomes: The co-primary outcomes were device-measured physical activity expressed in counts per minute at 12 months and falls rate over 12 months. Secondary outcomes included the proportion of fallers, device-measured daily steps and moderate-to-vigorous physical activity (MVPA), self-reported hours per week of physical activity, body mass index, eating habits, goal attainment, mobility-related confidence, quality of life, fear of falling, risk-taking behaviour, mood, well-being and disability., Results: The mean age of participants was 74 (SD 8) years, and 70% (n=425) were women. There was no significant effect of the intervention on device-measured physical activity counts per minute (mean difference 5 counts/min/day, 95% CI -21 to 31), or falls at 12 months (0.71 falls/person/year in intervention group and 0.87 falls/person/year in control group; incidence rate ratio 0.86, 95% CI 0.65 to 1.14). The intervention had a positive significant effect on device-measured daily steps and MVPA, and self-reported hours per week of walking, well-being, quality of life, and disability. No significant between-group differences were identified in other secondary outcomes., Conclusion: A physical activity and fall prevention programme including fall risk assessment and prevention advice, plus telephone-based health coaching, did not lead to significant differences in physical activity counts per minute or falls rate at 12 months. However, this programme improved other physical activity measures (ie, daily steps, MVPA, hours per week of walking), overall well-being, quality of life and disability., Trial Registration Number: ACTRN12615001190594., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY. Published by BMJ.)
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- 2024
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43. Monitoring falls in residential aged care facilities: Agreement between falls incident reports and progress notes.
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Dawson R, Feng A, Oliveira JS, Hassett L, Sherrington C, and Pinheiro MB
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- Aged, Humans, Female, Aged, 80 and over, Male, Risk Management, Residential Facilities, Pandemics, Homes for the Aged
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Objectives: Accurate fall reporting is essential for assessing the effectiveness of fall prevention strategies. This study aimed to investigate the level of agreement between incident reports and resident progress notes as data sources for falls monitoring in residential aged care facilities., Methods: A retrospective observational study was conducted involving 46 older people from six residential aged care facilities who had consented to join the broader TOP UP trial. Fall events documented in the incident report system and resident progress notes over 12 months before randomisation were extracted by two independent reviewers using a standardised Excel form. Agreement between the two data collection methods was calculated using Cohen's kappa coefficient., Results: A total of 75 falls were recorded from 27 (59%) of the 46 participants who were 65% female, with an average age of 83 [SD 9] years. The incident reports captured 68 (90.7%) falls, while the progress notes captured 73 (97.3%) falls. Overall, there was a 75% agreement between falls recorded in progress notes and incident reports. Perfect agreement was identified for five facilities (n = 35), while one facility had a lower agreement rate of 29% (n = 11), which appeared to be attributable to staff shortages linked to the COVID-19 pandemic., Conclusions: There was substantial agreement between incident reports and progress records. These findings support the use of incident reports for identifying falls in research or to investigate the effectiveness of fall prevention strategies in residential aged care facilities., (© 2024 The Authors. Australasian Journal on Ageing published by John Wiley & Sons Australia, Ltd on behalf of AJA Inc’.)
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- 2024
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44. Potential of coffee straw biochal as a substrate conditioner in seed lettuce and sorghum germination and vigority.
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Alixandre RD, Lima PAM, Almeida TFR, Oliveira JS, Pereira MB, Alixandre FT, Jacomino GRL, Dias RS, Alexandre RS, Ferreira A, Passos RR, and Lopes JC
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- Coffee, Edible Grain, Lactuca, Seeds physiology, Charcoal, Germination, Sorghum
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The use of residues from coffee production to obtain biochar is a sustainable approach, which aims to minimize the environmental impact of these materials. In this study, the effect of adding coffee straw biochar on the physiological quality of lettuce and sorghum seeds was investigated. Thus, the objective of this work was to study the effect of adding different concentrations of coffee biochar in the substrate composition on the physiological quality of lettuce (Lactuca sativa) and sorghum (Sorghum bicolor) seeds. The experimental design used was completely randomized, with five concentrations of biochar (0; 7.5; 15; 30 and 60%), conducted with four replications of 25 seeds. The use of biochar in the concentrations studied does not provide an increase in the average germination percentage and vigor of lettuce and sorghum seeds. The increase in the concentration of biochar caused less seed vigor, suggesting a toxic effect. For seed germination, there was no significant difference between lettuce and sorghum species, regardless of treatment. For the germination speed index, sorghum seeds have higher means, except for the treatment with the addition of 15% coffee straw biochar. Lettuce seeds have higher shoot length averages, except for treatment with 100% commercial substrate. The sorghum seeds have higher mean root length and dry mass than lettuce, regardless of the treatment.
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- 2024
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45. Primary results of the brazilian registry of atherothrombotic disease (NEAT).
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de Barros E Silva PGM, do Nascimento CT, Pedrosa RP, Nakazone MA, do Nascimento MU, de Araújo Melo L, Júnior OLS, Zimmermann SL, de Melo RMV, Bergo RR, Precoma DB, Tramujas L, Lima EG, Dantas JMM, do Amaral Baruzzi AC, Flumignan RLG, de Oliveira Paiva MSM, Gowdak LHW, de Carvalho PN, de Figueiredo Neto JA, Silvestre OM, Fioranelli A, Vieira RD', Horak ACP, Miyada DHK, Kojima FCS, de Oliveira JS, de Oliveira Silva L, Pavanello R, Ramacciotti E, and Lopes RD
- Subjects
- Aged, Female, Humans, Male, Brazil epidemiology, Prospective Studies, Registries, Risk Factors, Rivaroxaban therapeutic use, Middle Aged, Multicenter Studies as Topic, Observational Studies as Topic, Coronary Artery Disease drug therapy, Peripheral Arterial Disease epidemiology
- Abstract
There is limited contemporary prospective real-world evidence of patients with chronic arterial disease in Latin America. The Network to control atherothrombosis (NEAT) registry is a national prospective observational study of patients with known coronary (CAD) and/or peripheral arterial disease (PAD) in Brazil. A total of 2,005 patients were enrolled among 25 sites from September 2020 to March 2022. Patient characteristics, medications and laboratorial data were collected. Primary objective was to assess the proportion of patients who, at the initial visit, were in accordance with good medical practices (domains) for reducing cardiovascular risk in atherothrombotic disease. From the total of patients enrolled, 2 were excluded since they did not meet eligibility criteria. Among the 2,003 subjects included in the analysis, 55.6% had isolated CAD, 28.7% exclusive PAD and 15.7% had both diagnoses. Overall mean age was 66.3 (± 10.5) years and 65.7% were male patients. Regarding evidence-based therapies (EBTs), 4% were not using any antithrombotic drug and only 1.5% were using vascular dose of rivaroxaban (2.5 mg bid). Only 0.3% of the patients satisfied all the domains of secondary prevention, including prescription of EBTs and targets of body-mass index, blood pressure, LDL-cholesterol, and adherence of lifestyle recommendations. The main barrier for prescription of EBTs was medical judgement. Our findings highlight that the contemporary practice does not reflect a comprehensive approach for secondary prevention and had very low incorporation of new therapies in Brazil. Large-scale populational interventions addressing these gaps are warranted to improve the use of evidence-based therapies and reduce the burden of atherothrombotic disease.ClinicalTrials.gov NCT04677725., (© 2024. The Author(s).)
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- 2024
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46. Randomized Trial of Effect of Bariatric Surgery on Blood Pressure After 5 Years.
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Schiavon CA, Cavalcanti AB, Oliveira JD, Machado RHV, Santucci EV, Santos RN, Oliveira JS, Damiani LP, Junqueira D, Halpern H, Monteiro FLJ, Noujaim PM, Cohen RV, de Sousa MG, Bortolotto LA, Berwanger O, and Drager LF
- Subjects
- Humans, Female, Adult, Middle Aged, Male, Blood Pressure, Antihypertensive Agents therapeutic use, Obesity complications, Obesity surgery, Risk Factors, Treatment Outcome, Diabetes Mellitus, Type 2 complications, Diabetes Mellitus, Type 2 surgery, Diabetes Mellitus, Type 2 drug therapy, Bariatric Surgery, Gastric Bypass adverse effects, Gastric Bypass methods, Hypertension drug therapy, Hypertension epidemiology, Obesity, Morbid surgery
- Abstract
Background: Obesity represents a major obstacle for controlling hypertension, the leading risk factor for cardiovascular mortality., Objectives: The purpose of this study was to determine the long-term effects of bariatric surgery on hypertension control and remission., Methods: We conducted a randomized clinical trial with subjects with obesity grade 1 or 2 plus hypertension using at least 2 medications. We excluded subjects with previous cardiovascular events and poorly controlled type 2 diabetes. Subjects were assigned to Roux-en-Y gastric bypass (RYGB) combined with medical therapy (MT) or MT alone. We reassessed the original primary outcome (reduction of at least 30% of the total antihypertensive medications while maintaining blood pressure levels <140/90 mm Hg) at 5 years. The main analysis followed the intention-to-treat principle., Results: A total of 100 subjects were included (76% women, age 43.8 ± 9.2 years, body mass index: 36.9 ± 2.7 kg/m
2 ). At 5 years, body mass index was 36.40 kg/m2 (95% CI: 35.28-37.52 kg/m2 ) for MT and 28.01 kg/m2 (95% CI: 26.95-29.08 kg/m2 ) for RYGB (P < 0.001). Compared with MT, RYGB promoted a significantly higher rate of number of medications reduction (80.7% vs 13.7%; relative risk: 5.91; 95% CI: 2.58-13.52; P < 0.001) and the mean number of antihypertensive medications was 2.97 (95% CI: 2.33-3.60) for MT and 0.80 (95% CI: 0.51-1.09) for RYGB (P < 0.001). The rates of hypertension remission were 2.4% vs 46.9% (relative risk: 19.66; 95% CI: 2.74-141.09; P < 0.001). Sensitivity analysis considering only completed cases revealed consistent results. Interestingly, the rate of apparent resistant hypertension was lower after RYGB (0% vs 15.2%)., Conclusions: Bariatric surgery represents an effective and durable strategy to control hypertension and related polypharmacy in subjects with obesity. (GAstric bypass to Treat obEse Patients With steAdy hYpertension [GATEWAY]; NCT01784848)., Competing Interests: Funding Support and Author Disclosures This work was supported by grant #100238 from Ethicon, represented in Brazil by Johnson and Johnson do Brasil. Dr Schiavon has received a research grant from Ethicon; and has received lecture fees from Ethicon and Medtronic. Dr Cohen has received a research grant paid to the Institution from Johnson and Johnson, Medtech, and Medtronic; has received honoraria for lectures, presentations, and Speakers Bureau from Johnson and Johnson, Medtech, Medtronic, Janssen Pharmaceuticals, Novo Nordisk, and Abbott; and is a member of the Scientific Advisory Board for Baritek and GI Dynamics. Dr Drager has received lecture fees from Ethicon. All other authors have reported that they have no relationships relevant to the contents of this paper to disclose., (Copyright © 2024 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.)- Published
- 2024
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47. Use of behavior change techniques in physical activity programs and services for older adults: findings from a rapid review.
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Gilchrist H, Oliveira JS, Kwok WS, Sherrington C, Pinheiro MB, Bauman A, Tiedemann A, and Hassett L
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- Aged, Humans, Quality of Life, Randomized Controlled Trials as Topic, Behavior Therapy methods, Exercise
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Background: Understanding behavior change techniques (BCTs) used in randomized controlled trials (RCTs) of physical activity programs/services for older adults can help us to guide their implementation in real-world settings., Purpose: This study aims to: (a) identify the number and type of BCTs used in physical activity programs/services for older adults evaluated in large, good quality RCTs and (b) explore the impact of different BCTs on different outcome domains., Methods: This is a secondary data analysis of a WHO-commissioned rapid review of physical activity programs/services for older adults. Fifty-six trials testing 70 interventions were coded for the type and number of BCTs present using a published BCT taxonomy. The proportion of positive effects found from physical activity interventions using the most common BCTs was calculated for the outcomes of physical activity, intrinsic capacity, functional ability, social domain, cognitive and emotional functioning, and well-being and quality of life., Results: Thirty-nine of the 93 possible BCTs were identified in the included trials and 529 BCTs in total (mean 7.6, range 2-17). The most common BCTs were "action planning" (68/70 interventions), "instructions on how to perform a behavior" (60/70), "graded tasks" (53/70), "demonstration of behavior" (44/70), and "behavioral practice/rehearsal" (43/70). Interventions that used any of the most common BCTs showed overwhelmingly positive impacts on physical activity and social domain outcomes., Conclusion: Consideration of which BCTs are included in interventions and their impact on outcomes can improve the effectiveness and implementation of future interventions. To enable this, providers can design, implement, and evaluate interventions using a BCT taxonomy., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Society of Behavioral Medicine.)
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- 2024
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48. Forage cactus as an additive in corn without the cob silages of feedlot sheep diets.
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de Carvalho Sobral G, de Oliveira JS, Santos EM, de Araújo GGL, de Sousa Santos FN, Medeiros GR, de Souza EJO, de Sousa Vieira D, Leite GM, Coelho DFO, Santana LP, Gomes PGB, da Cunha Torres Júnior P, Santos MAC, and Viana NB
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- Animals, Female, Diet veterinary, Dietary Fiber, Digestion, Ethers, Lactation, Milk, Plant Extracts, Rumen, Sheep, Silage analysis, Weight Gain, Cactaceae, Zea mays
- Abstract
This study aimed to assess the impact of adding forage cactus as an additive to the production of corn silage without the cob on the performance of feedlot sheep and subsequent silage losses. The experimental design was completely randomized, consisting of three treatments: corn silage without cob; 0% = 100% corn plant without the cob; 10% = 90% corn plant without cob + 10% forage cactus; 20% = 80% corn plant without cob + 20% forage cactus. Significant effects were observed for dry matter intake (P = 0.0201), organic matter (P = 0.0152), ether extract (P = 0.0001), non-fiber carbohydrates (P = 0.0007). Notably, nutrient digestibility showed significant differences in organic matter (P = 0.0187), ether extract (P = 0.0095), neutral detergent fiber (P = 0.0005), non-fiber carbohydrates (P = 0.0001), and metabolizable energy (P = 0.0001). Performance variables, including total weight gain (P = 0.0148), average daily weight gain (P = 0.0148), feeding efficiency, and rumination efficiency of dry matter (P = 0.0113), also exhibited significant effects. Consequently, it is recommended to include 20% forage cactus in corn silage, which, based on natural matter, helps meet animals' water needs through feed. This inclusion is especially vital in semi-arid regions and aids in reducing silage losses during post-opening silo disposal., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
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- 2024
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49. Chemical Properties, Ruminal Fermentation, Gas Production and Digestibility of Silages Composed of Spineless Cactus and Tropical Forage Plants for Sheep Feeding.
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Godoi PFA, Magalhães ALR, de Araújo GGL, de Melo AAS, Silva TS, Gois GC, Dos Santos KC, do Nascimento DB, da Silva PB, de Oliveira JS, Santos EM, da Silva TGF, Zanine AM, Ferreira DJ, Voltolini TV, and Campos FS
- Abstract
The aim was to evaluate the chemical composition, carbohydrates, protein fractionation and in vitro gas production of silages composed of spineless cactus and tropical forages and their effect on sheep performance. Treatments consisted of silages: corn silage (CS), spineless cactus silage (SCS), spineless cactus + gliricidia (SCG), spineless cactus + buffel grass silage (SCBG) and spineless cactus + pornunça (SCP). Silos were opened 60 days after ensiling, and analyses were carried out. The digestibility test lasted for 36 days, with eight animals per treatment. A completely randomized design was adopted. Considering carbohydrate fractionation, CS, SCS and SCBG silages had higher total carbohydrate content ( p = 0.001). The SCS silage presented a higher A + B1 fraction ( p = 0.001). The SCBG and SCG silages showed a higher B2 fraction ( p < 0.0001) compared to the CS and SCS silages. The SCBG and SCP silages presented a higher C fraction ( p = 0.001). For protein fractionation, the SCP and SCG silages showed higher crude protein contents ( p = 0.001). The CS and SCS silages showed a higher A fraction ( p = 0.001). The SCBG silage presented a higher B1 + B2 fraction ( p = 0.001). The SCG silage showed a higher B3 fraction ( p = 0.006) compared to SCBG silage. The SCS and SCP silages showed a higher C fraction ( p = 0.001). Exclusive SCS silage showed higher in vitro dry matter digestibility ( p = 0.001), dry matter degradability ( p = 0.001) and total gas production ( p = 0.001). The use of the SCBG, SCP and SCG silages to feed sheep increased the dry matter intake ( p < 0.001). Sheep fed the SCG silage showed greater dry matter and crude protein digestibility compared to the sheep fed the CS, SCS and SCP silages ( p = 0.002). There was a higher water intake ( p < 0.001) with the use of the SCS and SCG silages to feed the sheep. The SCP and SCG silages provided a greater intake ( p < 0.001) and excretion ( p < 0.001) of nitrogen by the animals. Although there were no differences between the treatments for daily gains, lambs that received the spineless cactus-based silage associated with tropical forages showed higher gains (160-190 g/day) than lambs that received CS silage (130 g/day). Thus, the use of spineless cactus associated with buffelgrass, pornunça and gliricidia to prepare mixed silages (60:40) to feed sheep has potential use to feed sheep, with positive effects on nutrient degradation and increases in dry matter intake. Under experimental conditions, we recommend the exclusive use of spineless cactus silage associated with buffel grass, pornunça and gliricidia in feeding sheep in semi-arid regions, as it provides nutrients, water and greater daily gains compared to corn silage.
- Published
- 2024
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50. High-throughput analysis reveals disturbances throughout the cell caused by Arabidopsis UCP1 and UCP3 double knockdown.
- Author
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Lima RPM, Oliveira JS, do Nascimento LC, Labate MTV, Labate CA, Barreto P, and Maia IG
- Subjects
- Adipose Tissue, Brown metabolism, Aspartic Acid, Glutamates metabolism, Ion Channels metabolism, Mitochondrial Proteins genetics, Mitochondrial Proteins metabolism, Protein Isoforms metabolism, Uncoupling Protein 1 metabolism, Uncoupling Protein 3 metabolism, Arabidopsis genetics, Arabidopsis metabolism
- Abstract
Three genes encoding mitochondrial uncoupling proteins (UCPs) have been described in Arabidopsis thaliana (UCP1 to UCP3). In plants, UCPs may act as an uncoupler or as an aspartate/glutamate exchanger. For instance, much of the data regarding UCP functionality were obtained for the UCP1 and UCP2 isoforms compared with UCP3. Here, to get a better understanding about the concerted action of UCP1 and UCP3 in planta, we investigated the transcriptome and metabolome profiles of ucp1 ucp3 double mutant plants during the vegetative phase. For that, 21-day-old mutant plants, which displayed the most evident phenotypic alterations compared to wild type (WT) plants, were employed. The double knockdown of UCP1 and UCP3, isoforms unequivocally present inside the mitochondria, promoted important transcriptional reprogramming with alterations in the expression of genes related to mitochondrial and chloroplast function as well as those responsive to abiotic stress, suggesting disturbances throughout the cell. The observed transcriptional changes were well integrated with the metabolomic data of ucp1 ucp3 plants. Alterations in metabolites related to primary and secondary metabolism, particularly enriched in the Alanine, Aspartate and Glutamate metabolism, were detected. These findings extend our knowledge of the underlying roles played by UCP3 in concert with UCP1 at the whole plant level., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Masson SAS. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
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