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10 results on '"Omaru M. Kabia"'

2. Bifunctional fluorescent probes for detection of amyloid aggregates and reactive oxygen species

Author

Lisa-Maria Needham, Judith Weber, James W. B. Fyfe, Omaru M. Kabia, Dung T. Do, Ewa Klimont, Yu Zhang, Margarida Rodrigues, Christopher M. Dobson, Sonia Ghandi, Sarah E. Bohndiek, Thomas N. Snaddon, and Steven F. Lee

Subjects
fluorophores, ros, sensors, protein aggregation, single-molecule imaging, thioflavin t, Science
Abstract

Protein aggregation into amyloid deposits and oxidative stress are key features of many neurodegenerative disorders including Parkinson's and Alzheimer's disease. We report here the creation of four highly sensitive bifunctional fluorescent probes, capable of H2O2 and/or amyloid aggregate detection. These bifunctional sensors use a benzothiazole core for amyloid localization and boronic ester oxidation to specifically detect H2O2. We characterized the optical properties of these probes using both bulk fluorescence measurements and single-aggregate fluorescence imaging, and quantify changes in their fluorescence properties upon addition of amyloid aggregates of α-synuclein and pathophysiological H2O2 concentrations. Our results indicate these new probes will be useful to detect and monitor neurodegenerative disease.

Published
2018
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3. Si-Directed regiocontrol in asymmetric Pd-catalyzed allylic alkylations using C1-ammonium enolate nucleophiles

Author

Thomas N. Snaddon, James W. B. Fyfe, Omaru M. Kabia, and Colin M. Pearson

Subjects
Allylic rearrangement, 010405 organic chemistry, Organic Chemistry, Enantioselective synthesis, Regioselectivity, 010402 general chemistry, 01 natural sciences, Biochemistry, Combinatorial chemistry, Article, 0104 chemical sciences, chemistry.chemical_compound, Tsuji–Trost reaction, chemistry, Nucleophile, Drug Discovery, Electrophile, Lewis acids and bases, Vinylsilane
Abstract

Cooperative catalysis enables the direct enantioselective α-allylation of linear prochiral esters using Si-substituted allyl electrophiles. The Si-substituent directs the regioselectivity of enantioselective bond formation and provides products containing synthetically versatile pentafluorophenyl ester and vinylsilane moieties. Critical to the efficacy of this process was the recognition that the ancillary ligand on palladium could be altered to prevent formation of a deleterious ether by-product, whilst retaining enantioselectivity through the Lewis base catalyst. Flexibility such as this is unique to cooperative catalysis events and provides efficient access to an array of enantioenriched products that are orthogonally functionalized and easily modified.

Published
2018

4. Bifunctional fluorescent probes for detection of amyloid aggregates and reactive oxygen species

Author

Steven F. Lee, Yu Zhang, Thomas N. Snaddon, Lisa-Maria Needham, Margarida Rodrigues, Ewa Klimont, Omaru M. Kabia, James W. B. Fyfe, Judith Weber, Sarah E. Bohndiek, Christopher M. Dobson, Dung T. Do, Sonia Ghandi, Needham, Lisa-Maria [0000-0002-8139-5862], Bohndiek, Sarah [0000-0003-0371-8635], Lee, Steven [0000-0003-4492-5139], and Apollo - University of Cambridge Repository

Subjects
0301 basic medicine, Fluorescence-lifetime imaging microscopy, Amyloid, single-molecule imaging, Protein aggregation, 010402 general chemistry, medicine.disease_cause, sensors, 01 natural sciences, protein aggregation, 03 medical and health sciences, chemistry.chemical_compound, medicine, fluorophores, lcsh:Science, Bifunctional, Multidisciplinary, ROS, Single Molecule Imaging, Fluorescence, 3. Good health, 0104 chemical sciences, 030104 developmental biology, chemistry, Benzothiazole, Biophysics, Thioflavin T, lcsh:Q, Oxidative stress
Abstract

Protein aggregation into amyloid deposits and oxidative stress are key features of many neurodegenerative disorders including Parkinson's and Alzheimer's disease. We report here the creation of four highly sensitive bifunctional fluorescent probes, capable of H 2 O 2 and/or amyloid aggregate detection. These bifunctional sensors use a benzothiazole core for amyloid localization and boronic ester oxidation to specifically detect H 2 O 2 . We characterized the optical properties of these probes using both bulk fluorescence measurements and single-aggregate fluorescence imaging, and quantify changes in their fluorescence properties upon addition of amyloid aggregates of α-synuclein and pathophysiological H 2 O 2 concentrations. Our results indicate these new probes will be useful to detect and monitor neurodegenerative disease.

Published
2018

5. Correction to ‘Bifunctional fluorescent probes for detection of amyloid aggregates and reactive oxygen species’

Author

Thomas N. Snaddon, Sonia Gandhi, Ewa Klimont, James W. B. Fyfe, Steven F. Lee, Lisa-Maria Needham, Margarida Rodrigues, Yu Zhang, Sarah E. Bohndiek, Christopher M. Dobson, Judith Weber, Omaru M. Kabia, Dung T. Do, Lee, Steven F [0000-0003-4492-5139], and Apollo - University of Cambridge Repository

Subjects
single-molecule imaging, Amyloid, Stereochemistry, sensors, 3101 Biochemistry and Cell Biology, protein aggregation, chemistry.chemical_compound, fluorophores, lcsh:Science, Bifunctional, chemistry.chemical_classification, Reactive oxygen species, Multidisciplinary, Chemistry, Correction, ROS, Fluorescence, Thioflavin T, lcsh:Q, Research Article, 31 Biological Sciences
Abstract

Protein aggregation into amyloid deposits and oxidative stress are key features of many neurodegenerative disorders including Parkinson's and Alzheimer's disease. We report here the creation of four highly sensitive bifunctional fluorescent probes, capable of H2O2 and/or amyloid aggregate detection. These bifunctional sensors use a benzothiazole core for amyloid localization and boronic ester oxidation to specifically detect H2O2. We characterized the optical properties of these probes using both bulk fluorescence measurements and single-aggregate fluorescence imaging, and quantify changes in their fluorescence properties upon addition of amyloid aggregates of α-synuclein and pathophysiological H2O2 concentrations. Our results indicate these new probes will be useful to detect and monitor neurodegenerative disease.

Published
2018
Full Text
View/download PDF

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