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Currently, the negative effects of unified and intensive agriculture are of growing concern. To mitigate them, the possibilities of using local but nowadays underused crop for food production should be more thoroughly investigated and promoted. The soybean is the major crop cultivated for vegetable oil production in Zambia, while the oil production from local oil-bearing plants is neglected. The chemical composition of oils and cakes of a three traditional oil plant used by descendants of the Lozi people for cooking were investigated. Parinari curatellifolia and Schinziophyton rautanenii oils were chiefly composed of α-eleostearic (28.58–55.96%), linoleic (9.78–40.18%), and oleic acid (15.26–24.07%), whereas Ochna serrulata contained mainly palmitic (35.62–37.31%), oleic (37.31–46.80%), and linoleic acid (10.61–18.66%); the oil yield was high (39–71%). S. rautanenii and O. serrulata oils were rich in γ-tocopherol (3236.18 μg/g, 361.11 μg/g, respectively). The O. serrulata oil also had a very distinctive aroma predominantly composed of p-cymene (52.26%), m-xylene (9.63%), γ-terpinene (9.07%), o-xylene (7.97), and limonene (7.23%). The cakes remaining after oil extraction are a good source of essential minerals, being rich in N, P, S, K, Ca, and Mg. These plants have the potential to be introduced for use in the food, technical, or pharmaceutical industries.

Recently, Pacific nations have faced to alarming increase in prevalence of noncommunicable diseases connected with consumption of non-traditional processed food. It is believed that re-introduction of native diet may mitigate these negative trends. One of the traditional staple food of Pacific region are seeds of underutilized leguminous tree Inocarpus fagifer. Nevertheless, information on their chemical composition and nutritional properties are missing. Therefore we decided to analyze this crop for the presence of fatty acids, minerals, phenolics and vitamins. Performed analyses revealed a slightly predominating portion of unsaturated (e.g. 18:2 n-6; 18:1 n-9 and 18:3α n-3) over saturated (e.g. C18 and C16) fatty acids. Considering minerals, the substantial concentrations of copper, magnesium, manganese, potassium and zinc (19.32; 1823.21; 8.44; 23308.41 and 77.99 mg kg-1 of dry matter respectively) were recorded. Ferulic and coumaric acids were the most abundant phenolics (3.23 and 1.48 mg kg-1 of dry matter respectively), whereas flavonoids, isoflavonoids and coumestrol were also present. Regarding vitamins, niacin and riboflavin were found in respective concentrations 131.80 and 4.47 mg kg-1 of dry matter. Our findings suggest I. fagifer seeds as a prospective food source of several health-beneficial constituents which might contribute to the well-being of Pacific islanders. The online version of this article (doi: 10.5073/JABFQ.2016.089.034) contains a supplementary file.

This study aimed to examine the changes in selected soil properties at Lítov spoil heap (Sokolov, Czech Republic) and compare the current situation with the situation described twenty years ago. A total of 110 soil samples were taken at Lítov at the same sites as in 1998. The analyses of basic soil characteristics involved: exchangeable soil pH (pHKCl), organic carbon content (Cox), quality of humic substances (A400/A600), exchangeable acidity (Ea), and two types of aluminium contents in the soil. Changes in all soil characteristics between 1998 and 2018 were statistically evaluated, compared, and visualized using Geographical Information Systems (GIS). We have observed an increase of pHKCl, Cox and a slight improvement in humus quality compared to the results from 1998. The temporal changes of soil characteristics were evident in the whole area, and the influence of reclamation methods was also pronounced. Soil development close to the regional common natural conditions was found in the area where agricultural reclamation measures (i.e., covering with topsoil) were carried out. Furthermore, afforestation - mainly by deciduous trees - supported the improvement of soil characteristics favourable for plant growth. High pyrite content and marshland were identified as the main causes that led to vegetation cover mortality.

The composition of soil organic matter is considered to have a key influence on C sequestration and global climate change and can be associated with changes in vegetation cover in the terrestrial ecosystem. Our study aimed to evaluate the soil chemical structures and various organic components from available or reactive to more stable forms in forest soils affected by acidification and after conversion from fairly close to natural beech (Fagus sylvatica) stands to a spruce (Picea abies) monoculture. Our results revealed that the beech stands had higher contents of dissolved organic carbon and low molecular mass organic acid compared to the spruce stands. The aliphatic CH groups within the soluble alkaline-extractable organic substance (AEOS) gradually disappeared with deeper soil horizons under both forest species, while the presence of aliphatic CH groups in the low-solubility AEOS was more pronounced in the A horizon under spruce and relatively increased with depth under beech stands. The carboxylic groups were more prevalent in deeper soil horizons, while polysaccharide chains and nitrogen functional groups decreased with depth under both forest stands but were more prevalent under beech than under spruce stands. These findings suggest that the stability of organic matter through the forest soil profiles increased due to the transformation of various organic compounds from litter to more stable organic matter with higher amounts of lignin components to greater amounts of carboxylic groups and aromatic groups in deeper soil horizons. Furthermore, a higher number of mobile components of soil organic matter and carboxylic acids, together with lower pH and cation exchange capacity under spruce, resulted in the leaching of nutrients, releasing risk elements into the soil solution and accelerating the podzolization process.

Abstract Mining and smelting activities can contaminate soils and affect farming due to high emissions and input of potentially toxic elements (PTE) into the environment. Soils (sampled from two depths) and market vegetables from vegetable gardens located within the vicinity of unconfined slag deposits from decades of mining and smelting activities in Kutná Hora, Czechia were assessed to determine to what extent they pose a health hazard to communities that use these gardens. Pseudo-total As concentrations in the soils exceeded background levels (4.5 mg kg−1) 1.9–93 times, with higher concentrations in the deeper layer. The pseudo-total concentrations of PTE in soils ranked in the order As > Zn > Cd > Pb. Phyto-available concentrations of PTE in soils were relatively low, compared to pseudo-total concentrations. Concentration of As, Cd, Pb and Zn in the vegetables exceeded guideline values, with the highest concentrations found in the fruits of cucumber, peppers, and zucchini. Despite low phyto-available PTE concentrations in soils, all the PTE concentrations in the vegetables surpassed the guidelines set by the Czech Ministry of Health and EU directive, indicating a health hazard to consumers.

This study aims to compare the chromium (Cr) mobility in two soils with different Cr sources. The first one is an anthropogenically contaminated soil from an area of a piston ring production factory, and the second one is a serpentine soil with a naturally increased Cr level from the National Nature Reserve Mohelno serpentine steppe in the Czech Republic. To assess the influence of naturally occurring environmental factors on the Cr mobility, two sets of column leaching experiments were constructed, namely an acidified water treatment (AW) and a simulated root exudate treatment (SRE). It was found that the anthropogenically contaminated soil leached out many times more Cr for both (the AW and SRE) treatments in comparison with the serpentine soil. Generally, all the samples showed enhanced leaching from the bottom layer, respectively the horizon, for both treatments. Only the anthropogenic soil showed some differences in the bottom layer, which was more susceptible to the AW treatment than to the SRE one. These findings show the high dependence of the leachability of Cr on the Cr content origin. It has been proven that naturally enriched sites where elevated levels of Cr originate from the bedrock are more resistant to Cr leaching than anthropogenically contaminated soils. Feasible causes of these differences are discussed in this study.

The methodology described here presents the procedures for determining physical soil properties of undisturbed soil samples. Besides describing the methods for determining bulk and particle density, moisture content and porosity of the soil in detail, it also offers a way of determining soil's water holding properties when there is no pressure membrane apparatus available. This method is based on a capillary water saturation experiment and gravimetric measurements performed in different time intervals after the saturation (30 minutes, 2 hours, and 24 hours). With a few, simple to follow steps, and not using complicated and space-consuming equipment, it can be replicated in almost any laboratory, and the results are easily interpreted. The method was, and still is, widely used in the Czech Republic, and some parts of it are used as standard soil testing methods. To a lesser or greater detail, this method is described in Rejšek (1999), Valla et al. (2011), Pospíšilová et al. (2016) and ÚKZÚZ (2016), and this methodology is compiled from those publications, mainly focusing (and using the same abbreviations) on the procedures described by Valla et al. (2011). The methodology described does not essentially differ from the original, but the steps here have been described to a greater detail, based on the practical experiences obtained over the years, in order to make some common mistakes less likely to happen. The methodology is further complemented with graphical illustrations for each step described in the process, making it clearer, more easily understood, and easier to replicate. Since this methodology has not been available in English so far, this guide offers a great opportunity of its replication on an international level. • Simple, cost-effective and environmentally friendly method for determining physical soil properties • Easy replication and results interpretation • Results can be obtained even in non-highly specialized soil laboratories

Abstract Unhealthy soils in peri-urban and urban areas expose individuals to potentially toxic elements (PTEs), which have a significant influence on the health of children and adults. Hundred and fifteen (n = 115) soil samples were collected from the district of Frydek Mistek at a depth of 0–20 cm and measured for PTEs content using Inductively coupled plasma—optical emission spectroscopy. The Pearson correlation matrix of the eleven relevant cross-correlations suggested that the interaction between the metal(loids) ranged from moderate (0.541) correlation to high correlation (0.91). PTEs sources were calculated using parent receptor model positive matrix factorization (PMF) and hybridized geostatistical based receptor model such as ordinary kriging-positive matrix factorization (OK-PMF) and empirical Bayesian kriging-positive matrix factorization (EBK-PMF). Based on the source apportionment, geogenic, vehicular traffic, phosphate fertilizer, steel industry, atmospheric deposits, metal works, and waste disposal are the primary sources that contribute to soil pollution in peri-urban and urban areas. The receptor models employed in the study complemented each other. Comparatively, OK-PMF identified more PTEs in the factor loadings than EBK-PMF and PMF. The receptor models performance via support vector machine regression (SVMR) and multiple linear regression (MLR) using root mean square error (RMSE), R square (R2) and mean square error (MAE) suggested that EBK-PMF was optimal. The hybridized receptor model increased prediction efficiency and reduced error significantly. EBK-PMF is a robust receptor model that can assess environmental risks and controls to mitigate ecological performance.

Open-cast coal mining is one of the most often-debated industries in the world. Due to the significant environmental and health issues it causes, many of these sites have been reclaimed over the years, and many scientific publications and research has followed. In this paper, we have tried to assess the trends in recent research performed on reclaimed coal mining sites (RMS) by analyzing the publications visible on Web of Science (WoS) between 2015 and 2020 and dividing the research into six categories. The results show that there is a trend of rapid increase in research that deals with carbon and its pooling, nutrients, vegetation, and microbiology, and a significant decline in research on RMS soil physical properties, whereas other categories have shown an increasing but relatively steady trend. The application of modern technologies is also discussed. China, the USA, and India are the countries that quantitatively take the lead in coal RMS research, with India slowly overtaking the US in more recent years.

Open-cast coal mining presents a big global issue because of the large areas the mines occupy, which get entirely changed. Their ecosystems lose most of their functions, and a huge amount of fertile soil gets utterly destroyed. Reclamation is a process of returning the functions of the soil after the excavation is finished, most commonly achieved by establishing vegetation, which can sometimes be very difficult. This happens due to the physical, chemical and biological changes that occur on these sites, which are described in this paper. Also, some directions for mitigating these problems are given. Once the vegetation is successfully introduced, natural cycles that were compromised by the mining are established once again, and the process of soil formation begins. Some trends and problems related to pedogenesis research on reclaimed mine sites are presented and discussed, along with presumptions of how the process of soil formation evolves on afforested clayey Technosols of central Europe. The potential future research which would confirm these presumptions is discussed, with the emphasis on the need of research performed on older reclamation sites, as well as sites with similar ecological conditions and different tree species cover.

ABSTRACT: The study aimed to assess laying performance, growth rate, and bone quality properties of tibia and femur bones of various genotypes of laying hens, including determining essential element composition at the end of the laying cycle in smallholder conditions. The study included three genotypes of laying hens; Czech golden spotted (CGS), White Leghorn (LE) and Dominant Partridge D300 (D300) hens. In total, 180 hens (60/genotype) were used in 3 replications (20 hens/replication). The eggs were collected to determine egg lay and hen-day egg production. Additionally, feed consumption was recorded to determine feed consumption per day or egg, resp. The mortality rate was recorded. Hens were individually weighed every 10 wk to analyze the growth performance and body weight changes during the laying cycle. The differences in performance characteristics were observed as significant in all studied parameters. The bone quality analysis consisted of the determination of bone weight, length, width, and fracture toughness. Furthermore, dry matter, ash, and selected elements, which included boron (B), calcium (Ca), cadmium (Cd), copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn), sodium (Na), phosphorus (P), lead (Pb), and zinc (Zn) were assessed. Regarding the results of tibia and femur bones, the effect of genotype was determined as significant in all evaluated properties. In terms of element composition, all evaluated elements significantly differed among the genotypes in the tibia (with one exception of Cu) and in the femur (with one exception of Cd). In conclusion, our results showed that hens’ performance, production quality, mortality and bone properties significantly differed among genotypes under smallholder conditions. Thus, every genotype needs to be carefully considered, when the rearing conditions are set.

The objective of this research was to evaluate the influence of the housing system (deep litter [DL] vs. deep litter with swimming pond [DLSP]) on productive performance, carcass traits, body temperature, blood profile, and the element composition of the femur and tibia in Muscovy ducks. At 5 weeks of age, sexed ducklings (264) were divided into 4 equal groups according to housing system and gender (drakes vs. ducks). The groups were as follows: 66 drakes/DL, 66 drakes/DLSP, 66 ducks/DL, and 66 ducks/DLSP. Each of the four groups was divided into three identical replicated subgroups of 22 animals. Regarding external body temperature, the DL birds had higher temperatures compared with the DLSP birds. In addition, drakes had lower temperature values than ducks. Regarding the blood analysis, the birds did not manifest any deviations in the biochemical traits of the blood. The DLSP birds had greater live weight, weight gain, and feed conversion ratio, but a lower proportion of breast meat than the DL birds. The housing conditions did not affect the fracture toughness of the tibia and femur of the birds; however, Muscovy ducks from the DLSP group had more Ca and Mg in the tibia and more Mg in the femur compared with the DL birds.

The aim of the study was to evaluate the effect of clay as an amendment to decrease the mobility and plant availability of potentially toxic elements (PTEs). Kaolinite and vermiculite were added at a 9% application rate to a contaminated garden soil from Kutná Hora. Half of the original soil was acidified by the addition of HNO3, to observe the effect of the pH on the adsorption of the PTEs. The samples were analysed four times over an eight-week period to evaluate the effect of the clay additions on the As, Cd, Pb and Zn plant availability and different fractions of the BCR sequential extraction. The results indicate that the PTEs were mainly bound by ion/cation exchange on the edges of the clay minerals and onto the Fe/Mn oxides and organic fractions in the soils. The significance (P < 0.05) of the pH was observed for the plant availability of the PTE, especially on the As and Cd. A comparison of the vermiculite in the form of chunks and as powder displayed the most statistically significant results, where the vermiculite powder significantly decreased the plant availability of the As, Cd and Pb, while the opposite effect was observed for the Zn.

Soil microorganisms play a main role in the nutrient cycle and they also play an important role in soil health. This article studies the influence of three rates of biochar (0.5, 1 and 3%) in comparison with control (0 biochar) in two different soils (Valečov and Čistá) on soil microbiota activities. The biochar was prepared from 80% of digestate from Zea mays L. and 20% of cellulose fibres by pyrolysis (470°C, 17 min). The biochar ability to influence microbial processes in soil was determined by respiration and nitrification tests. There were no significant differences between basal respiration of control samples and biochar-amended samples. Basal respiration in the Valečov soil reached average amounts from 1.32 to 1.52 mg CO2/h/100 g. In the Čistá soil, basal respiration reached average amounts from 1.40 to 1.49 mg CO2/h/100 g. No significant differences were proved also in nitrification tests of both soils. Nitrifying potential was the highest in 3% rate of biochar amendment. There were no negative changes in the measured soil parameters. CO2 efflux was not higher in biochar-amended soil.

Genistein (GEN) and zearalenone (ZEA), environmental oestrogens commonly present in feedstuff for pigs, are known for their effects on reproductive functions. The aim was to verify the in vitro effects of 0.5-20 µM concentrations of GEN, ZEA and its metabolite α-zearalenol (α-ZOL) on pig sperm motility. A dose-dependent increase of the immotile sperm amount against fast and medium-fast sperm clusters was observed with all three oestrogens from the lowest concentrations tested. Individual CASA (computer-assisted sperm analysis) parameters of motile sperms seemed to be less sensitive indicators. This should be considered especially in toxicological research on a sperm model. Background of inconsistencies in to date-published papers is discussed. The results shift the effective concentrations of ZEA, α-ZOL, and GEN to values achievable in vivo and raises the questions of risk assessment of these compounds in pig reproduction.

Soil contamination by potentially toxic elements (PTEs) is intensifying under increasing industrialization. Thus, the ability to efficiently delineate contaminated sites is crucial. Visible–near infrared (vis–NIR: 350–2500 nm) and X-ray fluorescence (XRF: 0.02–41.08 keV) spectroscopic techniques have attracted tremendous attention for the assessment of PTEs. Recently, the application of fused vis–NIR and XRF spectroscopy, which is based on the complementary effect of data fusion, is also increasing. Moreover, different data manipulation methods, including feature selection approaches, affect the prediction performance. This study investigated the feasibility of using single and fused vis–NIR and XRF spectra while exploring feature selection algorithms for the assessment of key soil PTEs. The soil samples were collected from one of the most heavily polluted areas of the Czech Republic and scanned using laboratory vis–NIR and XRF spectrometers. Univariate filter (UF) and genetic algorithm (GA) were used to select the bands of greater importance for the PTE prediction. Support vector machine (SVM) was then used to train the models using the full-range and feature-selected spectra of single sensors and their fusion. It was found that XRF spectra alone (primarily GA-selected) performed better than single vis–NIR and fused spectral data for predictions of PTEs. Moreover, the prediction models that were derived from the fused data set (particularly the GA-selected) enhanced the models’ accuracies as compared with the single vis–NIR spectra. In general, the results suggest that the GA-selected spectra obtained from the single XRF spectrometer (for As and Pb) and from the fusion of vis–NIR and XRF (for Pb) are promising for accurate quantitative estimation detection of the mentioned PTEs.

The interactions of different organic acids such as citric, malic, oxalic, and fulvic with aluminium were studied using ion-exchange chromatography (IC) and high performance liquid chromatography (HPLC). The experiments were carried out at low pH (1.5-3.1). The results of IC experiments on the interaction between Al and oxalate, citrate, and malate were compared with model chemical equilibrium calculations. The strongest effect on Al speciation was observed with oxalic acid and fulvic acid. These two ligands formed more than one type of complex with Al. In contrast, there was no significant effect of malic acid on Al speciation and a rather weak effect caused by citric acid. The studies show that the formed complexes are stable even at low pH.

Forest soils near industrial zones can be endangered by acid deposition and by dust deposition containing potentially toxic elements (PTEs). Soils of the study area are acidified and the surface enrichment with Cd, Cu, Pb, and Zn reflects anthropogenic contamination. Two forms of all PTEs were evaluated: potentially mobilized (2M nitric acid extraction) and mobile (0.01M CaCl2 extraction) - the most toxic form. Negligible amounts of Cu and Pb were found in the mobile form. Pb mobilization is decreased by co-emitted bases and Cu mobilization is mainly controlled by soil reaction. These elements represent just a potential risk for the ecosystem. The mobile forms of Cd, Zn, and Mn account for approximately 30% of potentially mobilized forms in organic horizons. These elements could pose a problem to ecosystem vitality. Cd is toxic at small concentration and its content in mobilized form approaches the critical load. Cd can be considered the most dangerous element in the study area. Zn concentration is not reaching the limit value. Mobilization of Zn and Pb is mainly controlled by Ca and Mg content. The highest concentrations of Mn were found in the mineral horizons. It predicates a geogenic origin. The lowest percentage of the mobile form is in mineral horizon and its mobilization is controlled mainly by pH. Based on these results, a direct damage of forest by PTE contamination in the Silesian Beskids can be excluded. Lower contamination level along with acid condition and P deficiency could act as a permanent stress factor. Stressed forest is more predisposed e.g. to frost or insect damage.

The individual adsorption/desorption of Cu, Pb, and Zn in six soil samples taken from organic horizons of acid forest soils is studied in batch experiments. These three metals were chosen because of the varying extent of their sorption to the soil organic matter. The initial concentration range for all metals is 0-200 mg/l. 0.01 mol/l Ca(NO3)2 was used as the background electrolyte. Soil samples were taken at random locations throughout a relatively small area and are characterized by small differences in mineralogical composition. Organic matter content and pH of soils are considered as the key factors causing differences in sorption/desorption behaviour of selected metals. In general, the sorption of all the three metals increased with a combination of higher pH value and increasing organic matter content. Anyway, the extent of the influence of these two characteristics on sorption behaviour of metals is different, for desorption significant differences were not determined. Copper sorption seems to reflect mainly the differences in organic matter content in addition to the differences in pH. The average amount of sorbed Pb was approximately 90%, Cu sorption averaged 60%, and that of Zn 30%. Desorption of Pb into 0.01 mol/l Ca(NO3)2 remained at approximately 4%, for Zn at 30%, and desorption of Cu reached up to 13% of the amount adsorbed. All the studied soils proved effective at immobilizing lead and copper, but zinc was relatively highly released from these soils, even when the sorbed amount was minimal. Different vegetational background of these samples (either beech or spruce forests) does not significantly influence the sorption extent of these metals as the lower pH of samples taken under spruce stands is probably compensated by higher organic matter content in these samples. The experimental data are fitted by the Freundlich equation and the parameters of this equation together with the adsorption and desorption efficiency are used for comparison of the behaviour of all the three metals among the six soils.

Serious pollution of alluvial soils by a wide range of potentially toxic elements is usually observed in the valleys with mining and metallurgical industry. To outline areas of increased risk elements inputs, measurements of soil magnetic susceptibility can be used. This method is based on the measurement of the concentration of ferrimagnetic minerals of anthropogenic origin, mainly iron oxides, which are closely associated with risk elements such as heavy metals. The aim of this study is to examine the link between magnetic susceptibility of poorly drained Litavka River alluvial soils located in the mining/smelting region of Příbram (Czech Republic) and the observed high concentration of risk elements in soils. Surface volume magnetic susceptibility and the vertical distribution of magnetic susceptibility in soil profiles were measured in situ. On the basis of field measurements, selected parts of soil cores were sampled for a detailed magnetic and chemical analysis. Our results demonstrate a statistically significant correlation between magnetic susceptibility and soil concentration of Cu, Pb, and Zn. The magnetically enhanced soil layer, relatively low frequency-dependent susceptibility, presence of Verwey transition, and scanning electron microscope observations suggested a prevalence of coarse magnetite/maghemite, likely of anthropogenic origin. However, the magnetic properties in-situ also reflected the natural soil conditions and soil processes. The high content of organic matter, varying reductive/oxidative condition, and vertical migration of magnetic phases were the main factors influencing the observed magnetic susceptibility values.

North Bohemia belongs to one of the most heavily industrialized and polluted regions in Europe. The enormous acid deposition which culminated in the 1970s has largely contributed to the accelerated acidification process in the soils and consequently to the wide forest decline in North Bohemian mountains. In this paper we map the active topsoil pH and humus quality with ordinary, universal, and regression kriging and compare the accuracy of resulting maps with cross-validation. For the regression kriging we use two types of spatially exhaustive auxiliary information, first the altitude derived from digital elevation model and second the land cover classes derived from satellite imagery. The leave-one-out (cross-validation) statistics, i.e. mean error, root mean squared error, and mean squared deviation ratio, are taken for comparison since they are widely accepted as measurements of the accuracy of digital soil maps. The results show that the regression kriging is superior over other kriging methods in this case. Out of 97 sampling sites the regression kriging with land cover classes is the best predictor at 32 sites for pH and at 30 sites for humus quality, the regression kriging with altitude at 31 and 25 sites, the universal kriging at 21 and 23 sites, and the ordinary kriging at 13 and 18 sites. The highest number of best predictions for regression kriging implies that the topsoil pH and humus quality are driven approximately equally by land cover and altitude and little less by pure geographic position. Furthermore, the universal kriging maps show a northeast to southwest spatial trend of topsoil pH and a northwest to southeast spatial trend for humus quality.

Shredded card (SC) was assessed for use as a sorbent of potentially toxic elements (PTE) carried from contaminated soil in various leachates (oxalic acid, formic acid, CaCl2, water). We further assessed SC for retention of PTE, using acidified water (pH 3.4). Vertical columns and a peristaltic pump were used to leach PTE from soils (O and A/B horizons) before passing through SC. Sorption onto SC was studied by comparing leachates, and by monitoring total PTE contents on SC before and after leaching. SC buffers against acidic soil conditions that promote metals solubility; considerable increases in solution pH (+4.49) were observed. Greatest differences in solution PTE content after leaching with/without SC occurred for Pb. In oxalic acid, As, Cd, Pb showed a high level of sorption (25, 15, and 58x more of the respective PTE in leachates without SC). In formic acid, Pb sorption was highly efficient (219x more Pb in leachate without SC). In water, only Pb showed high sorption (191x more Pb in leachate without SC). In desorption experiments, release of PTE from SC varied according to the source of PTE (organic/mineral soil), and type of solvent used. Arsenic was the PTE most readily leached in desorption experiments. Low As sorption from water was followed by fast release (70% As released from SC). A high rate of Cd sorption from organic acid solutions was followed by strong retention (~12% Cd desorption). SC also retained Pb after sorption from water, with subsequent losses of ≤8.5% of total bound Pb. The proposed use of this material is for the filtration of PTE from extract solution following soil washing. Low-molecular-mass organic acids offer a less destructive, biodegradable alternative to strong inorganic acids for soil washing.

A set of fifty samples were taken from soil surface layers of an anonymous vineyard in the Bohemia region of the Czech Republic. Samples were analysed for basic soil parameters including pH and humus content and quality and for potentially toxic elements Cu, Pb, Zn, Cd and Mn. When compared to soil guideline values, mean Cu exceeded the limit by 280%. Although other elements did not exceed the guideline value, Zn and Cd surpassed background concentrations by 108 and 187%, respectively. Mn did not occur in concentrations that are considered excessive with respect to common natural soil levels. A statistically significant correlation was observed for Cu and Cox, but no significant correlation could be made between Cu and humus quality, suggesting that in this case, Cu retention in surface soil is more dependent on quantity of humic substances rather than humus quality. The unnaturally high accumulation of Cu in the surface horizon is certainly of anthropogenic origin; most likely due to long term application of copper-based fungicides. High observed values of Cd are probably a result of the soils parent material and application of fertilizers which may have contained cadmium compounds. Distribution maps illustrated a common trend of potentially toxic element accumulation in the western section of the study area plot. However, many variables which were unmeasured in this study could account for the distribution and therefore more detailed study is required before conclusions can be made regarding this occurrence. An analysis of wine produced at the vineyard showed that although the end product beverage contained insignificant amounts of Cu in both red and white wines, the white wine contained 0.026 mg/l of Cd; a value which could contribute considerably to exceeding the dietary allowable intake of cadmium. The investigation should be extended to analyse a wider range of wines produced from the vineyard with a focus on soil-plant-fruit-wine transfer of cadmium and/or production processes.

A high displacement of inhabitants into large towns, presence of industry, and constantly growing traffic have a high impact on the environment and considerable exposure of human health to environmental risks. Therefore, putting emphasis on the best environmental quality is necessary. In this work, the pollution level of urban parks was studied, the influence of the type of pollution source was analysed, and the effect of shading by trees was studied. The analyses were carried out on soil samples taken from thirteen parks in two towns of the Czech Republic, in Prague, a town considered to be mainly residential, and Ostrava, a predominantly industrial town (steel working plant). The sampling points were selected to cover the whole towns equally. In each park, two sampling points were chosen, the first one under trees, the second one in the open area. The sampling was done in the summer of 2006 in the depths of 0-10 and 10-20 cm. In addition to basic soil analyses performed by routine methods, potentially risk elements (Zn, Cd, Pb, Cu, and As) in cold 2M HNO3 extract were determined. Differences between the sampling points shaded and not shaded by trees were evidenced, with higher concentrations of risk elements under trees. The element contents differed between both towns as well. Significantly higher values of lead (mean 86 mg/kg) and copper (mean 28 mg/kg) were found in Prague, as a traffic consequence, compared to Ostrava, where lead reached the mean of 41 mg/kg and copper of 18 mg/kg. Maximum permissible limits were exceeded in Ostrava parks especially with Cd, in Prague with Pb.

The chlorotoluron transport in the soil profiles was studied under field conditions on three different soil types of the Czech Republic. The herbicide was applied on 21. 4. 2005 on a four-square meter plot using an application rate of 2 kg/ha. Soil samples were taken on days 35 and 150 from the herbicide application to study the remaining chlorotoluron distributions in the soil profiles. The chlorotoluron distributions in the monitored soils were very similar 35 days after the herbicide application. The major part of chlorotoluron was detected in the top layer of the soil profile (0-8 cm). The highest concentration was obtained in the top 2 cm layer and it decreased gradually with the depth. The percentages of the remaining chlorotoluron 35 days after the herbicide application were similar in Haplic Luvisol (29.97%) and Greyic Phaeozem (30.78%), and slightly higher in Haplic Cambisol (38.58%). The chlorotoluron distributions in the monitored soils differed considerably 150 days after the herbicide application. Chlorotoluron was detected in the entire monitored soils profiles (0-50 cm). The highest concentration was found in all cases in the top 2 cm layer and it decreased gradually with the depth to the depth of approximately 10 cm. Below this level, the herbicide contents were low and the values oscillated randomly. The percentages of the remaining chlorotoluron 150 days after the herbicide application were in the increasing order: Greyic Phaeozem < (5.45%) < Haplic Luvisol (11.7%) < Haplic Cambisol (17.48%). The BPS mathematical model connected with the soil database was used to simulate the chlorotoluron distribution 35 and 150 days after the herbicide application. The comparison of the measured and simulated data indicated probably varying chlorotoluron half-life during the experiment. The results showed that, if the chlorotoluron half-life is estimated based on the remaining chlorotoluron content in the soil profile on the 150th day after the herbicide application, the simulated herbicide content on the 35th day is twice as high as the measured one. On the other hand, if the half-life degradation of chlorotoluron is estimated based on the remaining chlorotoluron content in the soil profile on the 35th day, the herbicide is almost totally degraded on the 150th day of the model simulation.

Aluminium (Al) mobilisation in the forest soils is a serious problem due to the soil acidification. The rate and magnitude of leaching of Al and other elements and compounds from soils can be examined by means of percolation experiments. Aluminium elutriation was studied under laboratory conditions using undisturbed samples of forest topsoil from the Paličník area in the Jizera Mountains (Czech Republic), which originated under two different vegetation covers: European beech (Fagus sylvatica L.) and Norway spruce (Picea abies (L.) Karst). Ponding infiltration was performed using three subsequently applied solutions. KCl solution was used to simulate the soil solution. Solutions with sulphates and nitrates addition (of two different pH values) were used to simulate acid rainfall. Passing liquid phase was analysed with respect to Al content and aluminium speciation. Differences were found in Al content and transport between different soils under spruce and beech covers. The soil sample under the spruce forest (SF sample) had a higher initial Al content than the soil sample under the beech forest (BF sample). As a result, the aluminium leaching from the spruce soil sample and the final content of water-extractable Al in the soil (Al content after the leaching experiment) were higher compared to the beech soil sample. This suggests that Al mobility and potential toxicity in the beech forest are grater than those in the spruce monoculture when studied in the acidification endangered areas.

Background and aimsHigh Al resistance of Rumex obtusifolius together with its ability to accumulate Al has never been studied in weakly acidic conditions (pH > 5.8) and is not sufficiently described in real soil conditions. The potential elucidation of the role of organic acids in plant can explain the Al tolerance mechanism.MethodsWe established a pot experiment with R. obtusifolius planted in slightly acidic and alkaline soils. For the manipulation of Al availability, both soils were untreated and treated by lime and superphosphate. We determined mobile Al concentrations in soils and concentrations of Al and organic acids in organs.ResultsAl availability correlated positively to the extraction of organic acids (citric acid < oxalic acid) in soils. Monovalent Al cations were the most abundant mobile Al forms with positive charge in soils. Liming and superphosphate application were ambiguous measures for changing Al mobility in soils. Elevated transport of total Al from belowground organs into leaves was recorded in both lime-treated soils and in superphosphate-treated alkaline soil as a result of sufficient amount of Ca available from soil solution as well as from superphosphate that can probably modify distribution of total Al in R. obtusifolius as a representative of "oxalate plants." The highest concentrations of Al and organic acids were recorded in the leaves, followed by the stem and belowground organ infusions.ConclusionsIn alkaline soil, R. obtusifolius is an Al-hyperaccumulator with the highest concentrations of oxalate in leaves, of malate in stems, and of citrate in belowground organs. These organic acids form strong complexes with Al that can play a key role in internal Al tolerance but the used methods did not allow us to distinguish the proportion of total Al-organic complexes to the free organic acids.