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The CuO-mesoporous silicon oxide nanocomposites are widely used in the adsorption and degradation of environmental pollutants, attributing to the effective photogenerated electrons and holes and high specific surface area of mesoporous silicon oxide. In this papers, Cu(NO3)2 was introduced during the assembly process of mesoporous silicon oxide to prepare CuO/MCM-41 nanocomposites by one-step method, which significantly simplified the preparation process compared to post-synthesis transplantation method. The prepared CuO/MCM-41 nanocomposites was characterized by Brunauer–Emmett–Teller (BET), Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscope (FE-SEM), Energy-dispersive X-ray Spectroscopy (EDS) and X-ray Diffraction (XRD). CuO/MCM-41 nanocomposites had excellent degradation effect under UV irradiation of 12W on different structures dyes, such as Methylene Blue(MB), Direct Fast Scarlet 4BS, Direct Yellow 27 and Reactive Turquoise Blue KN-G. It was demonstrated that the kinetic studies of photocatalytic degradation of MB were fitted well with the first-order kinetic model. The degradation of MB retained 97% after five cycles reusing of the catalyst. The results indicated that CuO/MCM-41 nanocomposites could be used as an effective photocatalyst for the degradation of different types of dyes in wastewater. [ABSTRACT FROM AUTHOR]

Introduction: Mycoplasma pneumoniae (MP) is the major cause of respiratory infections that threaten the health of children and adolescents worldwide. Therefore, an early, simple, and accurate detection approach for MP is critical to prevent outbreaks of MP-induced community-acquired pneumonia. Methods: Here, we explored a simple and accurate method for MP identification that combines loop-mediated isothermal amplification (LAMP) with the CRISPR/Cas12b assay in a one-pot reaction. Results: In the current study, the whole reaction was completed within 1 h at a constant temperature of 57°C. The limit of detection of this assay was 33.7 copies per reaction. The specificity of the LAMP-CRISPR/Cas12b method was 100%, without any cross-reactivity with other pathogens. Overall, 272 clinical samples were used to evaluate the clinical performance of LAMP-CRISPR/Cas12b. Compared with the gold standard results from real-time PCR, the present method provided a sensitivity of 88.11% (126/143), specificity of 100% (129/129), and consistency of 93.75% (255/272). Discussion: Taken together, our preliminary results illustrate that the LAMPCRISPR/Cas12b method is a simple and reliable tool for MP diagnosis that can be performed in resource-limited regions. [ABSTRACT FROM AUTHOR]

Large congenital lid colobomas are traditionally repaired using 1- or 2-step vascularized flap-graft combinations. However, visual axis occlusion for weeks is a severe problem in small children and recent reports suggest that the flap pedicle does not contribute to blood perfusion. A "one-step" substitute for large lid defects has recently been reported in animals and humans, demonstrating the viability of a bilamellar autograft alone. We present an alternative "one-step" reconstructive approach in a 6-month-old infant who had a centrally-located large upper eyelid defect resulting from a congenital coloboma. The free full-thickness bilamellar autograft was harvested from the contralateral upper eyelid. The follow-up time was 48 months. Cosmetic and functional results were good, the bilamellar graft survived, and there was no graft ischemia, necrosis, or rejection. The boy developed madarosis, lid notching, and mild contour irregularity but needed no reoperation since the parent was satisfied with the surgical result. A free bilamellar eyelid autograft seems to be an outstanding alternative to both "conventional 2-step" and "modern 1-step" options for the reconstruction of large colobomatous eyelid openings, especially in young infants who cannot tolerate visual axis blockage. It is an easy, practical, fast, and effective technique that also saves cost in health care. [ABSTRACT FROM AUTHOR]

The development of commercially viable composite conducting polymer electrodes for energy storage is limited by the requirement of multiple and complex fabrication steps, low energy density, and poor cycling stability. In this work, a straightforward, economical, single‐step method is developed for creating densely packed nanostructured PEDOT/graphene composite material demonstrating its application as an electrode for supercapacitors. The electrode achieved the highest mass loading reported so far in the literature for composite vapor phase polymerized PEDOT/rGO using aqueous FeCl3 (25.2 mg cm−2), and displayed an ultrahigh areal capacitance of 4628.3 mF cm−2 at 0.5 mA cm−2. The symmetric two‐electrode setup displayed an energy density of 169.3 µWh cm−2 and a 70% capacitance retention after 70 000 cycles, showcasing its exceptional performance and durability. [ABSTRACT FROM AUTHOR]

ABSTRACT Salmonella enterica serovar Typhimurium (ST) is a predominant serovar causing foodborne illnesses worldwide. Traditional detection methods often face challenges, including the need for specialized equipment, skilled operators, and lengthy procedures. To address these limitations, we developed a rapid, sensitive, and specific ST detection method by integrating loop-mediated isothermal amplification (LAMP) with the clustered regularly interspaced short palindromic repeats and associated protein 12b (CRISPR/Cas12b) system, all within a single tube. Our results indicate that the LAMP–CRISPR/Cas12b reaction can be completed isothermally in under 1 h without requiring specialized instruments. The platform’s limit of detection (LoD) is 12.5 copies per reaction. Additionally, the system demonstrated 100% inclusivity and exclusivity when tested against 30 reference strains, highlighting its specificity. In practical applications, the LoDs for ST in pure nucleic acid and contaminated fecal samples were 2.32 and 23.2 CFU/mL, respectively, with higher sensitivity observed in pure nucleic acid samples. Overall, our findings underscore the potential of the one-tube LAMP–CRISPR/Cas12b platform as a rapid, sensitive, and specific tool for ST detection, particularly in resource-limited settings.IMPORTANCEHere, we have provided a novel one-step method for Salmonella Typhimurium detection in one pot by integrating the LAMP assay with the CRISPR/Cas12b system, offering significant advantages in terms of simplicity, speed, and accuracy.

Background: Percutaneous nephrolithotomy (PCNL) is the first line treatment for treating kidney stones larger than 2 cm size with the advantages of lower morbidity and faster post-operative recovery. Creation of a nephrostomy access is one of the major steps of PCNL. It can be done either by single-step dilatation or by multi-step serial dilatation technique. Objectives: To compare the outcome and evaluate the efficacy of percutaneous nephrolithotomy done by single-step versus serial dilatation technique with specific reference to access time, fluoroscopy time, rate of blood transfusion, length of hospital stay (post-op) and complication rate. Methods: It was a prospective study with a sample size of 100 where 50 patients underwent one-step dilatation and other 50 patients underwent serial-dilatation technique. These patients were analysed on the basis of their demographic profile, renal access time, total fluoroscopy time for access, post-operative haematuria, duration of post-operative hospital stay and complications after operative procedure. Data was analysed and reported using statistical tools. Results: Demographic profile of the patients and stone characteristics were equivalent between two groups. Mean access tract dilatation time, mean total fluoroscopy time were significantly less in one of the groups. Requirement of blood transfusion, and post-operative complications rate were not significant between two groups. Conclusion: Both methods are safe and effective for tract dilatation. However, PCNL can be successfully performed by one-step dilatation with the added advantages of lesser time of renal access, lesser radiation exposure. Blood loss and hospital stay after operative procedure were not statistically significant between two groups. [ABSTRACT FROM AUTHOR]

A series of quaternary ammonium or phosphonium salts were applied as zeolite growth modifiers in the synthesis of hierarchical ZSM-5 zeolite. The results showed that the use of methyltriphenylphosphonium bromide (MTBBP) could yield nano-sized hierarchical ZSM-5 zeolite with a "rice crust" morphology feature, which demonstrates a better catalytic performance than other disinfect candidates. It was confirmed that the addition of MTBBP did not cause discernable adverse effects on the microstructures or acidities of ZSM-5, but it led to the creation of abundant meso- to marco- pores as a result of aligned tiny particle aggregations. Moreover, the generation of the special morphology was believed to be a result of the coordination and competition between MTBBP and Na+ cations. The as-synthesized hierarchical zeolite was loaded with Zn and utilized in the propane aromatization reaction, which displayed a prolonged lifetime (1430 min vs. 290 min compared with conventional ZSM-5) and an enhanced total turnover number that is four folds of the traditional one, owing to the attenuated hydride transfer reaction and slow coking rate. This work provides a new method to alter the morphological properties of zeolites with low-cost disinfectants, which is of great potential for industrial applications. [ABSTRACT FROM AUTHOR]

Point-of-care sensors targeting blood marker analysis must be designed to function with very small volumes since acquiring a blood sample through a simple, mostly pain-free finger prick dramatically limits the sample size and comforts the patient. Therefore, we explored the potential of converting a conventional lateral flow assay (LFA) for a significant biomarker into a self-contained and compact polymer channel-based LFA to minimize the sample volume while maintaining the analytical merits. Our primary objective was to eliminate the use of sample-absorbing fleece and membrane materials commonly present in LFAs. Simultaneously, we concentrated on developing a ready-to-deploy one-step LFA format, characterized by dried reagents, facilitating automation and precise sample transport through a pump control system. We targeted the detection of the heart failure biomarker NT-proBNP in only 15 µL human whole blood and therefore implemented strategies that ensure highly sensitive detection. The biosensor combines streptavidin-functionalized magnetic beads (MNPs) as a 3D detection zone and fluorescence nanoparticles as signal labels in a sandwich-based immunoassay. Compared to the currently commercialized LFA, our biosensor demonstrates comparable analytical performance with only a tenth of the sample volume. With a detection limit of 43.1 pg∙mL−1 and a mean error of 18% (n ≥ 3), the biosensor offers high sensitivity and accuracy. The integration of all-dried long-term stable reagents further enhances the convenience and stability of the biosensor. This lateral flow channel platform represents a promising advancement in point-of-care diagnostics for heart failure biomarkers, offering a user-friendly and sensitive platform for rapid and reliable testing with low finger-prick blood sample volumes. [ABSTRACT FROM AUTHOR]

Although several separation membranes for the purification of dye-containing oily wastewater have been reported, achieving continuous one-step separation and photodegradation of complex oil/water effluents containing dyes is still a significant challenge. In previous studies, the purification of oil/water mixtures has typically involved a two-step process involving the initial separation of oil/water mixtures followed by the subsequent photodegradation of organic dyes. This is because one-step purification requires the membrane to concurrently possess an excellent separation efficiency and photodegradation ability to ensure efficient degradation of dyes as they rapidly pass through the separation membrane. Herein, we propose a photothermally responsive poly-(N-isopropylacrylamide)-MXene/polyacrylonitrile-poly-(N-isopropylacrylamide) nanofibrous hydrogel membrane (PNIPAm-MXene/PAN-PNIPAm, PMPP membrane). The PMPP membrane is composed of a PNIPAm-MXene hydrogel bonded to the surface of a PAN-PNIPAm nanofibrous membrane. The PNIPAm-MXene hydrogel layer imparts excellent photothermally responsive wettability, photodegradation activity, and antifouling properties to the membrane. The PAN-PNIPAm nanofibrous membrane provides a porous structure that ensures the highly efficient separation of complex oil/water mixtures. The PMPP membrane achieves simultaneous one-step separation and photodegradation of complex oil/water effluents under light irradiation, particularly in the case of multiphase heavy oil/water/light oil mixtures containing both oily and water-soluble organic dyes and oil–water emulsions. The degradation and separation efficiencies of the PMPP membrane exceed 99.5%. Even after 20 cycles, the degradation rates of Sudan IV and methyl blue (MB) remain above 95.7 and 99.9%, respectively. [ABSTRACT FROM AUTHOR]

Multiple impurities removal represents one of the most daunting challenges in gas purification, but the attainment of efficient adsorptive separation is hindered by the difficulty in designing adsorbents that could simultaneously capture different impurities. Herein, we revealed a molecular trap within Zn‐trz‐ox (trz = 1,2,4‐triazole; ox = oxalic acid) that featured positive H and negative O sites, and the suitable pore size, which exhibited remarkable one‐step C2H4 purification performance directly from quaternary C2H4/C2H2/C2H6/CO2 mixtures. The separation selectivities of C2H4 with respect to CO2, C2H2, and C2H6 are 9.8, 2.6, and 2.5, higher than the sole adsorbent yet reported. Meanwhile, polymer grade C2H4 (≥99.95%) could be directly obtained with record C2H4 productivity of 1.5 mol kg−1, over 10 times higher than that of the previous benchmark material. The deep insight into the binding behavior revealed by simulation studies offers important clues for the design of advanced adsorbent for multiple impurities capture. [ABSTRACT FROM AUTHOR]

Biomass shows intrinsic superiorities to produce hierarchical porous activated carbon with microwave heating. However, biomass is incapable of absorbing microwave energy, and additional microwave absorbers must be added. Herein, a crucible capable of absorbing microwave waves was employed to simplify the process to produce a hierarchical corncob-based porous carbon (HCCPC) using a microwave-assisted one-step physical–chemical coupled activation method without additional microwave absorber being added. The resulting HCCPC possessed a high specific surface area of 1948 m2/g, a high mesopore ratio of 35.5%, and a high graphitization degree of IG/ID = 1.55. The corresponding capacitive performances of HCCPC-based symmetrical two-electrode supercapacitors (SCs) were investigated in aqueous, organic, and ionic liquid electrolytes. The SC with KOH electrolyte showed the highest specific capacity of 281 F/g at 0.5 A/g and the most outstanding cyclic durability of 100% at 0.5 A/g after 10,000 cycles. Meanwhile, the SC with 1-ethyl-3-methylimidazolium tetrafluoroborate (EMIMBF4) ionic liquid electrolyte could obtain the highest energy density of 48.2 Wh/kg at a power density of 900 W/kg. These results demonstrate that employing a crucible capable of absorbing microwave energy in the microwave-assisted one-step physical–chemical coupled activation is universal and feasible to prepare biomass-based hierarchical porous carbon for high-performance SCs. [ABSTRACT FROM AUTHOR]

Objective. X-ray spectral computed tomography (CT) allows for material decomposition (MD). This study compared a one-step material decomposition MD algorithm with a two-step reconstruction MD algorithm using acquisitions of a prototype CT scanner with a photon-counting detector (PCD). Approach. MD and CT reconstruction may be done in two successive steps, i.e. decompose the data in material sinograms which are then reconstructed in material CT images, or jointly in a one-step algorithm. The one-step algorithm reconstructed material CT images by maximizing their Poisson log-likelihood in the projection domain with a spatial regularization in the image domain. The two-step algorithm maximized first the Poisson log-likelihood without regularization to decompose the data in material sinograms. These sinograms were then reconstructed into material CT images by least squares minimization, with the same spatial regularization as the one step algorithm. A phantom simulating the CT angiography clinical task was scanned and the data used to measure noise and spatial resolution properties. Low dose carotid CT angiographies of 4 patients were also reconstructed with both algorithms and analyzed by a radiologist. The image quality and diagnostic clinical task were evaluated with a clinical score. Main results. The phantom data processing demonstrated that the one-step algorithm had a better spatial resolution at the same noise level or a decreased noise value at matching spatial resolution. Regularization parameters leading to a fair comparison were selected for the patient data reconstruction. On the patient images, the one-step images received higher scores compared to the two-step algorithm for image quality and diagnostic. Significance. Both phantom and patient data demonstrated how a one-step algorithm improves spectral CT image quality over the implemented two-step algorithm but requires a longer computation time. At a low radiation dose, the one-step algorithm presented good to excellent clinical scores for all the spectral CT images. [ABSTRACT FROM AUTHOR]

Purpose: Laparoscopic adjustable gastric band (LAGB) has high technical and weight loss failure rates. We evaluate here the 1-year morbidity, mortality, and weight loss of laparoscopic Roux-en-Y-gastric bypass (LRYGB) as a feasible conversion strategy. Methods: Patients with a failed primary LAGB who underwent LRYGB from July 2004 to December 2019 were selected from an electronic database at our center. Patients had a conversion to LRYGB at the same time (one-stage approach) or with a minimum of 3 months in between (two-stage approach). Primary outcomes included 30-day morbidity and mortality. Secondary outcomes were body mass index (BMI), percent excess weight loss (%EWL), and percent excess BMI lost (%EBMIL) at 1 year postoperatively. Results: A total of 1295 patients underwent a conversion from LAGB to LRYGB at our center: 1167 patients (90.1%) in one stage and 128 patients (9.9%) in two stages. There was no mortality. An early (30-day) postoperative complication occurred in 93 patients (7.2%), with no significant difference found between groups. Hemorrhage was the most common complication in 39 patients (3.0%), and the reoperation was required in 19 patients (1.4%). At 1 year postoperatively, the mean BMI was 28.0 kg/m2, the mean %EWL 72.8%, and the mean %EBMIL 87.0%. No statistically significant difference was found between the groups. Conclusion: Conversion to LRYGB can be considered as a safe and effective option with low complication rate and good weight loss outcomes at 1 year. One-stage conversion provides the same early outcome as two-step surgery with a competent surgeon. [ABSTRACT FROM AUTHOR]

Factor mixture modeling (FMM) has been increasingly used in behavioral and social sciences to examine unobserved population heterogeneity. Covariates (e.g., gender, race) are often included in FMM to help understand the formation and characterization of latent subgroups or classes. This Monte Carlo simulation study evaluated the performance of one-step and three-step approaches to covariate inclusion across three scenarios, i.e., correct specification (study 1), model misspecification (study 2), and model overfitting (study 3), in terms of direct covariate effects on factors. Results showed that the performance of these two approaches was comparable when class separation was large and the specification of covariate effect was correct. However, one-step FMM had better class enumeration than the three-step approach when class separation was poor, and was more robust to the misspecification or overfitting concerning direct covariate effects. Recommendations regarding covariate inclusion approaches are provided herein depending on class separation and sample size. Large sample size (1000 or more) and the use of sample size-adjusted BIC (saBIC) in class enumeration are recommended. [ABSTRACT FROM AUTHOR]

New structural sheet metal parts are developed in an iterative, time-consuming manner. To improve the reproducibility and speed up the iterative drawability assessment, we propose a novel low-dimensional multi-fidelity inspired machine learning architecture. The approach utilizes the results of low-fidelity and high-fidelity finite element deep drawing simulation schemes. It hereby relies not only on parameters, but also on additional features to improve the generalization ability and applicability of the drawability assessment compared to classical approaches. Using the machine learning approach on a generated data set for a wide range of different cross-die drawing configurations, a classifier is trained to distinguish between drawable and non-drawable setups. Furthermore, two regression models, one for drawable and one for non-drawable designs are developed that rank designs by drawability. At instantaneous evaluation time, classification scores of high accuracy as well as regression scores of high quality for both regressors are achieved. The presented models can substitute low-fidelity finite element models due to their low evaluation times while at the same time, their predictive quality is close to high-fidelity models. This approach may enable fast and efficient assessments of designs in early development phases at the accuracy of a later design phase in the future. [ABSTRACT FROM AUTHOR]

A series of quaternary ammonium or phosphonium salts were applied as zeolite growth modifiers in the synthesis of hierarchical ZSM-5 zeolite. The results showed that the use of methyltriphenylphosphonium bromide (MTBBP) could yield nano-sized hierarchical ZSM-5 zeolite with a “rice crust” morphology feature, which demonstrates a better catalytic performance than other disinfect candidates. It was confirmed that the addition of MTBBP did not cause discernable adverse effects on the microstructures or acidities of ZSM-5, but it led to the creation of abundant meso- to marco- pores as a result of aligned tiny particle aggregations. Moreover, the generation of the special morphology was believed to be a result of the coordination and competition between MTBBP and Na+ cations. The as-synthesized hierarchical zeolite was loaded with Zn and utilized in the propane aromatization reaction, which displayed a prolonged lifetime (1430 min vs. 290 min compared with conventional ZSM-5) and an enhanced total turnover number that is four folds of the traditional one, owing to the attenuated hydride transfer reaction and slow coking rate. This work provides a new method to alter the morphological properties of zeolites with low-cost disinfectants, which is of great potential for industrial applications.

Conditional knockout mice are valuable tools for examining the functions of targeted genes in a time‐ and space‐specific manner. Here, we generated gene‐edited mice by using the Tol2 transposon to introduce guide RNA (gRNA) into fertilized eggs obtained by crossing LSL (loxP‐stop‐loxP)‐CRISPR‐associated 9 (Cas9) mice, which express Cas9 in a Cre‐dependent manner, with CAG‐CreER mice. Transposase mRNA and plasmid DNA, which contained a gRNA sequence for the gene encoding tyrosinase flanked by the transposase recognition sequence, were injected together into fertilized eggs. As a result, the transcribed gRNA cleaved the target genome in a Cas9‐dependent manner. Using this method, it is possible to generate conditional genome‐edited mice more easily in a shorter period of time. [ABSTRACT FROM AUTHOR]

N-doped reduced graphene oxide quantum dots (N-rGQDs) have attracted more and more attention in efficient catalytic degradation of aqueous organic pollutants. However, the synthesis of N-rGQDs is generally a complex and high energy required process for the reduction and N-doping steps. In this study, a facile and green fabrication approach of N-rGQDs is established, based on a metal-free Fenton reaction without additional energy-input. The N structures of N-rGQDs play a significant role in the promotion of their catalytic performance. The N-rGQDs with relatively high percentage of aromatic nitrogen (NAr-rGQDs) perform excellent catalytic activities, with which the degradation efficiency of pollutant is enhanced by 25 times. Density functional theory (DFT) calculation also indicates aromatic nitrogen structures with electron-rich sites are prone to transfer electron, presenting a key role in the catalytic reaction. This metal-free Fenton process provides a green and cost-effective strategy for one-step fabrication of N-rGQDs with controllable features and potential environmental catalytic applications.

Core-shell microspheres have attracted widespread attention in the field of drug delivery due to their special morphology and excellent surface properties. However, the preparation of the core–shell microspheres composed of hydroxyapatite (HA) have been rarely reported. In this paper, porous HA core–shell microspheres were prepared through a one-step hydrothermal method by using sodium trimetaphosphate (STMP) as the phosphorus source. SEM and TEM observations showed that the prepared porous HA core–shell microspheres were composed of a porous shell and a solid core, with a gap between the core and the shell. These microspheres bear uniform size and identical structure. With the increase of the hydrothermal time, the solid microspheres transformed to porous core–shell microspheres with the simultaneous transformation of their phase from amorphous calcium phosphate (ACP) to HA. The possible formation mechanism is proposed, providing inspiration for the design of porous HA core–shell microspheres and their potential biological applications.

Home testing technology strategy is critical for early screening of disease. However, current home testing technologies often require complex processes, which limits their application. In this study, a time-resolved cascade logic gate microfluidic chip (TCLMC) was revealed to enable capillary force-based one-step operation without manual intervention or professional equipment. By analogy with logic gates in the circuit, TCLMC could automatically control the fluid flow and regulate the incubation time to optimize the immunoassay. The limit of detection of TCLMC for SARS-CoV-2 and influenza B virus (Flu B) was 134.94 and 79.17 pg mL−1 within 10 min. Additionally, this study tested saliva samples from 12 Flu B patients and 24 healthy controls to verify its clinical application. The results showed that TCLMC had high sensitivity (100%), specificity (100%), and accuracy (100%). This study provides a new one-step strategy for home testing and demonstrates its great potential in the diagnosis field. [ABSTRACT FROM AUTHOR]

The one-step green preparation of heterojunction-structured bifunctional high-efficiency electrocatalysts provides a simple method for the green, low-cost, convenient and efficient application of hydrogen energy, meanwhile it is still challenging to achieve. In the present work, the phosphorus-doped two-phase heterojunction P-Co 5.47 N/Co 9 S 8 @NPC nanorods was synthesized on carbon cloth (CC) as a high-quality bifunctional electrode through simple one-step chemical vapor deposition (CVD) method. Saccharomycetes cerevisiae were used for the non-toxic phosphorus doping and formation of a carbon nanolayer wrapping Co 5.47 N/Co 9 S 8 nanorods. Compared with the single-phase electrocatalyst Co 9 S 8 @NPC NSs/CC, P-Co 5.47 N/Co 9 S 8 @NPC NSs/CC exhibited more excellent electrocatalytic performance which required overpotentials of 69 mV for hydrogen evolution reaction (HER), 182.5 mV for oxygen evolution reaction (OER), 1.45 V for over water splitting (OWS) at 10 mA cm−2 in KOH solution. The designed P-Co 5.47 N/Co 9 S 8 @NPC NSs/CC presented multiple advantages of nanorod structure, doped structure, abundant heterointerfaces, as well as dual-phase synergy, which triggered outstanding electrocatalytic performance. [Display omitted] • P-Co 5.47 N/Co 9 S 8 @NPC heterostructure is synthesized by one-step preparation strategy. • P-Co 5.47 N/Co 9 S 8 @NPC NSs/CC catalyst exhibits superb electrocatalytic activity. • P-Co 5.47 N/Co 9 S 8 @NPC NSs/CC has nanorod structure and doped structure. • P-Co 5.47 N/Co 9 S 8 @NPC NSs/CC has abundant heterointerfaces and dual-phase synergy. [ABSTRACT FROM AUTHOR]

[Display omitted] • A DNA tweezer-assembled, regenerated nanosensor was proposed for viral RNA detection. • Rapid detection of viral RNA was achieved within 30 min. • Sustainable detection of viral RNA was achieved at least three cycles. • Determination of virus simulant was achieved. • Rapid and sustainable detection of heavy metal ion (Hg2+) was performed. Transcription-polymerase chain reaction (RT-qPCR) is the golden standard to detect viral RNA. However, it suffers from several inherent defects such as high false-negative rates, time-consuming and expensive. To exploit better analytical methods for future global pandemics, it is vital to develop rapid, sustainable, and environmentally-friendly sensing methods. Here, a one-step, regenerated nanosensor assembled by DNA tweezers is developed for rapid and sustainable detection of viral RNA. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA is selected as model analyte. The DNA tweezer is designed with a central strand and two arm strands containing overhangs complementary to SARS-CoV-2 RNA. The SARS-CoV-2 RNA hybridizes with the two arm overhangs and strains the DNA tweezer to closed conformation, resulting in the close proximity of the donor and acceptor fluorophores. With the addition of an anti-strand complementary to the SARS-CoV-2 RNA, the closed DNA tweezer is relaxed to open conformation through a strand displacement process, leading to the separation of the dual fluorophores. Thus, the DNA tweezer is revived for fresh SARS-CoV-2 RNA. The proposed nanosensor can be regenerated for three cycles with the same performance. Moreover, the viral RNA can be detected within 30 min with a detection limit of 0.04 nM. By altering the sequences of the DNA tweezer, rapid, sensitive, and sustainable detection of Hg2+ is achieved. The proposed nanosensor provides a novel approach for rapid, flexible, and sustainable detection of viral RNA and Hg2+, exhibiting great potential for routine preliminary coronavirus disease tests, epidemic control, and environmental monitoring. [ABSTRACT FROM AUTHOR]

As most HIV rapid tests (HRT) detect only HIV-1/2 antibodies, their performance during primary HIV infection is poor. Determine HIV Early detect (Abbott) (Determine) is the only HRT with an HIV-1 p24-antigen detection, but the impact of this addition in shortening the diagnostic window remains unclear. A total of 183 HIV-1 primary infection samples were tested using the HRTs Determine and ONE STEP anti-HIV (1&2) Test (InTec Products) (One-Step). The pre-seroconversion subgroup was defined as p24-antigen positivity without Western blot nor Liaison XL (fouth generation enzyme immunoassay with distinct signal for p24-antigen and HIV-1 antibody) HIV-1 antibodies. Global sensitivity (95% CI) was 95% (91-97) for Determine versus 80% (74%-85%) for One-Step (difference p = 1.38e-06). Pre-seroconversion subgroup sensitivity was lower, at 71.9 (54.6%-84.4%) for Determine and 9.7% (3.3%-24.9%) for One-Step. Among the 45 samples with an HIV-1 infection date, no HRT was reactive up to 2 weeks. Between 2 and 3 weeks, Determine sensitivity was 78% (45%-95%) versus 56% (27%-81%) for One-Step. From 3 weeks to 1 month Determine sensitivity was 90% (62%-98%) and One-Step 45% (21%-72%). The last negative sample occurred at 3 weeks for Determine versus 70-90 days for One-Step. HRT with p24-antigen detection significantly shortens the diagnostic window from approximatively 3 months to 1 month. HRTs should be used with caution in the first month after HIV infection., (© 2024 The Author(s). Journal of Medical Virology published by Wiley Periodicals LLC.)

Salmonella enterica serovar Typhimurium (ST) is a predominant serovar causing foodborne illnesses worldwide. Traditional detection methods often face challenges, including the need for specialized equipment, skilled operators, and lengthy procedures. To address these limitations, we developed a rapid, sensitive, and specific ST detection method by integrating loop-mediated isothermal amplification (LAMP) with the clustered regularly interspaced short palindromic repeats and associated protein 12b (CRISPR/Cas12b) system, all within a single tube. Our results indicate that the LAMP-CRISPR/Cas12b reaction can be completed isothermally in under 1 h without requiring specialized instruments. The platform's limit of detection (LoD) is 12.5 copies per reaction. Additionally, the system demonstrated 100% inclusivity and exclusivity when tested against 30 reference strains, highlighting its specificity. In practical applications, the LoDs for ST in pure nucleic acid and contaminated fecal samples were 2.32 and 23.2 CFU/mL, respectively, with higher sensitivity observed in pure nucleic acid samples. Overall, our findings underscore the potential of the one-tube LAMP-CRISPR/Cas12b platform as a rapid, sensitive, and specific tool for ST detection, particularly in resource-limited settings., Importance: Here, we have provided a novel one-step method for Salmonella Typhimurium detection in one pot by integrating the LAMP assay with the CRISPR/Cas12b system, offering significant advantages in terms of simplicity, speed, and accuracy., Competing Interests: The authors declare no conflict of interest.

Abstract Aim To compare universal screening with selective risk factor based screening for GDM, using the one-step 75 g oral glucose tolerance test (OGTT). Materials and method A cross-sectional, comparison between universal and selective risk factor based screening for GDM, among 400 antenatal care clients at Alex-Ekwueme Federal University Teaching Hospital Abakaliki (AE-FUTHA). All the participants had 75 g OGTT at 24–28 weeks of gestation and risk factor screening for GDM. All 400 participants formed the universal group while participants with one or more of the considered risk factors formed the selective risk factor group. Data were analyzed using IBM SPSS version 20. Statistical comparison was done using t- test for continuous variables. Logistics regression was used to determine the level of associations of the independent predictors for hyperglycemia. Level of significance was set at P

To overcome the intrinsic disadvantages of pristine g-C3N4 and make it reusable, improve the activity of g-C3N4 by making use of the near-infrared region, the magnetic Fe3O4/β-NaYF4/g-C3N4 (FO/UC/CN) heterogeneous materials with high and stable NIR enhanced photocatalytic activity were prepared via a new facile one-step solvothermal synthesis method. It can be revealed that FO/UC/CN can activate UV–visible light under excitation of 980 nm. Compared with pure Fe3O4, g-C3N4 and Fe3O4/g-C3N4, the obtained FO/UC/CN15 heterojunction exhibits good photodegradation rates of 65.56, 21.35, 59.22 and 20.49% for MB, MO, RhB and phenol, respectively. In addition, after four cycles of the photocatalytic degradation test, the photodegradation rates remain 62.14, 20.54, 57.64 and 19.36%, respectively. The results illustrate magnetic FO/UC/CN heterojunction exhibits highly NIR-enhanced photocatalytic activity and excellent stability. This study indicates a promising system to synthesize g-C3N4-based magnetic photocatalysis for effectively utilizing the NIR and treating industrial wastewater, which can be expected to alleviate the energy crisis and enhance the application of solar energy for dealing with environmental remediation in the future. [ABSTRACT FROM AUTHOR]

We propose a theoretical model for realizing a photonic two-qubit phase gate in cavity QED using a one-step process. The fidelity and probability of success of the conditional quantum phase gate is very high in the presence of cavity decay. Our scheme only employs one two-level atom, and thus is much simpler than other schemes involving multi-level atoms. This proposal can also be applied to generate two-mode squeezed states; therefore, we give three examples, i.e., the two-mode squeezed vacuum state, two-mode squeezed odd coherent state, and two-mode squeezed even coherent state, to estimate the variance of Duan's criterion when taking into account cavity decay. It is shown that the variance is smaller than 2 for the three squeezed states in most cases. Furthermore, we utilize logarithmic negativity to measure the entanglement, and find that these squeezed states have very high degrees of entanglement. [ABSTRACT FROM AUTHOR]

A novel method for detecting pesticide multi‐residue in grass forage (alfalfa and oat) was established based on the one‐step automatic extraction and purification technology of quick, easy, cheap, effective, rugged, and safe combined with ultrahigh‐performance liquid chromatography quadrupole Orbitrap high‐resolution mass spectrometry. The crushed sample was extracted with acetonitrile with 1% acetate, followed by a cleanup step with a primary‐secondary amine, octadecylsilane, and graphitized carbon black. The extraction and purification were carried out using the one‐step automatic pretreatment equipment. The target pesticides were acquired in positive ion electrospray ionization mode and full scan/data dependent secondary scan mode. The calibration curve shows good linearity over the corresponding concentration range, with the coefficient of determination greater than 0.99. The screening detection limits were 0.5–50 μg/kg, and the limit of quantification for the 206 pesticides was set at 1–50 μg/kg. At the spiking levels of one, two, and 10 times of limit of quantification, more than 95% of pesticides had recovery between 70–120%, with a relative standard deviation ≤20%. The method was proved to be simple, rapid, high‐sensitivity, and could be routinely used for the high throughput screening and quantitative analysis of pesticide residues in alfalfa and oat. [ABSTRACT FROM AUTHOR]

Multi-view clustering has attracted increasing attention for handling complex data with multiple views or sources. Among them, spectral clustering-based methods become more and more popular due to it can make full use of information from different views. However, most existing multi-view spectral clustering methods typically adopt a two-step scheme, which firstly obtains the spectral embedding matrix through graph fusion or multi-feature fusion, and then uses the k-means algorithm to cluster the spectral embedding matrix to obtain the final clustering result. This two-step scheme inevitably leads to information loss, resulting in a suboptimal solution. Furthermore, the methods of graph fusion and multi-feature fusion have not taken into account the inconsistency of features between different views and the unordered nature of clustering labels, which also decreases the clustering performance. To solve these problems, we propose a novel one-step multi-view spectral clustering based on multi-feature similarity fusion. This model simultaneously conducts graph learning, multi-feature similarity fusion and discretization in a unified framework, which can mutually negotiate and optimize each other to achieve better results. Furthermore, compared to directly fusing affinity matrices or spectral embedding matrixs from different views, we take advantage of the property of the spectral embedding matrix, fuse the similarity of samples in feature space, better handle the differences between different views. Finally, the superiority of our method is verified by the experimental evaluation of several data sets. The demo code of this work is publicly available at https://github.com/kong-de-zheng/MOMSC. [Display omitted] • Introduce block diagonal constraint to make the affinity matrix accurately reflecting the data structure. • Fuse the features from different views, preserving the local structure and the consistency of multi-views. • Propose an one-step framework to optimize graph learning, multi-feature fusion and clustering. [ABSTRACT FROM AUTHOR]

Point-of-care testing (POCT) technology, using lateral flow assays and microfluidic systems, facilitates cost-effective diagnosis, timely treatment, ongoing monitoring, and prevention of life-threatening outcomes. Aside from significant advancements demonstrated in academic research, implementation in real-world applications remains frustratingly limited. The divergence between academic developments and practical utility is often due to factors such as operational complexity, low sensitivity and the need for trained personnel. Taking this into consideration, our objective is to present a critical and objective overview of the latest advancements in fully integrated one-step POCT assays for home-testing which would be commercially viable. In particular, aspects of signal amplification, assay design modification, and sample preparation are critically evaluated and their features and medical applications along with future perspective and challenges with respect to minimal user intervention are summarized. Associated with and very important for the one-step POCT realization are also readout devices and fabrication processes. Critical analysis of available and useful technologies are presented in the SI section. [ABSTRACT FROM AUTHOR]

• Facile single-step processing of LTO by suspension plasma spraying demonstrated. • Suspension can be conveniently prepared using commercial LTO powder. • Spray parameters significantly influence LTO coating deposition. • Although partially delithiation is noted, LTO spinel phase largely retained. • Galvanostatic cycling of the coating showed the expected potential reaction. Spinel Li 4 Ti 5 O 12 (LTO) is a promising anode material for solid state thin film batteries (SSTB) due to its almost-zero volume change and promising Li-ion mobility. However, preparing LTO anodes for SSTB demands tedious vacuum-based processing steps that are not cost effective. In this context, the present study embarks on evaluating the versatile suspension plasma spraying (SPS) approach to fabricate LTO coatings without using any binder. The microstructure and stoichiometry of the fabricated LTO coatings developed through the SPS route reveals retention of ∼76 wt.% of the spinel LTO from the starting feedstock, with minor amounts of rutile and anatase TiO 2. The SPS experiments yielded varying thickness build up rates of the LTO coatings depending on the processing parameters adopted. The electrochemical data of the produced LTO based electrode tested in a half-cell through galvanostatic cycling show reversible lithiation and delithiation at expected potential, thereby validating the promise of the SPS technique for potential fabrication of SSTB components once fully optimized. [Display omitted] [ABSTRACT FROM AUTHOR]

Summary: A one-step activation technique for preparing activated carbon/titanium dioxide (AC-TiO2) photocatalyst was developed in this study, which achieved the expansion of activated carbon and the crystallization transition of titanium dioxide in one step. The properties of the generated materials were examined utilizing photocatalytic degradation of tetracycline hydrochloride (TC). The optimized sample (10%AC-TiO2) has a photocatalytic efficiency of 99.8% in 60 minutes and a degradation efficiency of more than 90% after 5 cycles. According to the experiment results, the combination of AC with TiO2 at high temperatures can induce a considerable number of oxygen vacancies in TiO2, hence increasing the reaction activation site, resulting in improved photocatalytic performance. A strong C-Ti chemical bond can be formed between AC and TiO2. Simultaneously, using AC as the substrate decreases the aggregation problem induced by the small nano size of TiO2 and increases dispersion uniformity. The unique carbon structure with a amount of pore structure can considerably improve the catalyst's adsorption efficacy. Because o f the synergistic effect of adsorption-degradation and the perfect photocatalytic activity of TiO2, the composite photocatalyst has exceptional photocatalytic efficiency, opening new possibilities for TiO2 modification. [ABSTRACT FROM AUTHOR]

It is a major constraint that complex synthesis methods limit the application of catalysts. Herein, Ag/BaTiO 3 catalyst is synthesized via a facile method, which demonstrates the outstanding catalytic properties. Investigations reveal that Ag-loading BaTiO 3 could increase carrier concentration, reduce the electronic impedance and enhance carrier's separation efficiency under ultrasonic vibration, leading to superior piezocatalytic performance. Therefore, Ag/BaTiO 3 catalyst presents the high degradation efficiency of 95.4 % in 50 min for RhB dye solution at light illumination and ultrasonic vibration. Moreover, when Ag content is 0.33 %, the degradation ratio of the sample still remains 81.9 % after 5th cycles, which indicates Ag/BaTiO 3 catalyst has good stability. This work provides a novel and easy implement strategy for designing high performance catalysts, and could be extended to other catalytic material. [Display omitted] [ABSTRACT FROM AUTHOR]

Due to their self-supporting and nanoparticulate structure, metal aerogels have emerged as excellent electrocatalysts, especially in the light of the shift to renewable energy cycles. While a large number of synthesis parameters have already been studied in depth, only superficial attention has been paid to the solvent. In order to investigate the influence of this parameter with respect to the gelation time, crystallinity, morphology, or porosity of metal gels, AuxCuy aerogels were prepared in water and ethanol. It was shown that although gelation in water leads to highly porous gels (60 m2g−1), a CuO phase forms during this process. The undesired oxide could be selectively removed using a post-washing step with formic acid. In contrast, the solvent change to EtOH led to a halving of the gelation time and the suppression of Cu oxidation. Thus, pure Cu aerogels were synthesized in addition to various bimetallic Au3X (X = Ni, Fe, Co) gels. The faster gelation, caused by the lower permittivity of EtOH, led to the formation of thicker gel strands, which resulted in a lower porosity of the AuxCuy aerogels. The advantage given by the solvent choice simplifies the preparation of metal aerogels and provides deeper knowledge about their gelation. [ABSTRACT FROM AUTHOR]

A programmed, autonomous, and self-powered DNA motor was developed for one-step amplification detection of ochratoxin A (OTA). The OTA can bind with the aptamer of hairpin and induce the opening of hairpin. Then, the Mg2+-specific DNAzyme can circularly cleave the fluorophore-labeled DNA on the gold nanoparticles (AuNPs), causing autonomous and processive movement of DNA motor. Finally, the cleaved fluorophore-labeled DNA segments can leave from the AuNP surface, causing significant recovery of fluorescent signal. A good linear range was obtained from 0.01 to 5 nM (R2 = 0.992) with a detection limit of 2 pM. Importantly, this strategy is self-powered and only one-step operation is needed for the whole process. It shows great potential in food security supervision or biological imaging of OTA. [ABSTRACT FROM AUTHOR]

Summary: An effective and eco‐friendly catalyst for hydrogen evolution reaction (HER) shows significance for new clean energy technologies. The TMPs are generally regarded as efficient electrocatalysts for HER. Unfortunately, the synthetic procedures of TMPs usually suffered from high cost, complexity, and toxicity caused by the use of chemical phosphorus sources. Phosphorus and nitrogen pollution lead to water eutrophication and consequent algae blooms. We use a kind of algae (caused by eutrophication) to develop TMP nanoparticles supported on the porous carbon matrix for HER by a one‐step calcination treatment, the preparation method is sustainable, economical, and effective. For the optimal catalyst Co2P nanoparticles supported on chlorella‐derived porous N‐doped carbon matrix (Co2P‐C‐NPC), the HER overpotential is measured to be 151 mV (acid condition) and 252 mV (alkaline condition) at the current density of 10 mA cm−2. The excellent HER performance results from the uniform dispersity, high content of carbon, phosphorus and nitrogen in the algae, the algae's high surface and porosity. If adverse element‐rich biomass can be converted into a useful catalyst material, then the efficient and eco‐friendly for water splitting is possible. [ABSTRACT FROM AUTHOR]

An effective base‐promoted cyclization and coupling strategy for the construction of substituted 3‐aryl‐5‐thio‐1,3,4‐thiadiazole‐2‐thiones from phenylhydrazines, olefins and CS2 is developed through employing CS2 as a sulfur feedstock. The reaction proceeds under mild reaction conditions in the absence of any external catalysts, transition metals and ligands. The one‐step three‐component procedure also shows the merits of simple operation, using available starting materials, wide substrate scopes, and high atom and step economy. This reaction provides facile access to substituted 3‐aryl‐5‐thio‐1,3,4‐thiadiazole‐2‐thiones, which could be used in the field of drug research. [ABSTRACT FROM AUTHOR]

In this study, a one-step electrochemical aptasensor was developed to detect the biomarker vascular endothelial growth factor (VEGF), an important protein in the pathogenesis of many retinal diseases, including age-related macular degeneration, diabetic retinopathy, retinopathy of prematurity, and retinal vein occlusion. The aptamer has a good affinity and can rapidly identify and capture VEGF based on its unique structure. We designed a VEGF aptasensor based on the aptamer recognition and complex metallo nanoenzyme particles as an electron exchange center and bridge between capture DNA and electrode. The aptamers maintained the hairpin structure to avoid nonspecific surface adsorption and expose the capture sequence outwards when the target was inexistent. Conversely, the aptamers opened the hairpin structure to release space to accomplish binding between VEGF and DNA, resulting in increased impedance. The performance of the electrochemical aptasensor is detected by electrochemical impedance spectroscopy (EIS). The limit of detection by EIS was as low as 8.2 pg ml−1, and the linear range was 10 pg ml−1–1 μg ml−1. The electrochemical aptasensor also showed high specificity and reproducibility.

Porcine circovirus type 3 (PCV3), a novel circovirus, imposes great burdens on the global pig industry. The penside tests for detecting PCV3 are critical for assessing the epidemiological status and working out disease prevention and control programs due to the unavailability of a commercial vaccine. A one-step molecular assay based on visual loop-mediated isothermal amplification (vLAMP) was developed for simple and rapid detection of PCV3. We compared its sensitivity and specificity with TaqMan quantitative real-time polymerase chain reaction (qPCR) and applied the developed assay in the epidemiological study of (n = 407) pooled swine sera collected from almost the entire mainland China during the years 2017–2018. We also explored the feasibility of the vLAMP assay for detecting raw samples without a prior DNA isolation step to expand its application capability. Results showed that the vLAMP assay could reliably detect the PCV3 cap gene with a detection limit of 10 DNA copies equal to that of the Taqman qPCR assay. In the epidemiological study, the PCV3 positive detection rate for 407 swine pooled sera detected by the vLAMP assay was 37.35% (152/407), whereas it was 39.01% (159/407) for Taqman qPCR. For the detection method without genome extraction, the results kept satisfactory specificity (100%) but displayed lower sensitivity (100% for CT < 32), indicating the direct detection is not sensitive enough to discriminate the samples with low viral loads. The one-step vLAMP is a convenient, rapid, and cost-effective diagnostic for penside detection and will enable the epidemiological surveillance of PCV3, which has widely spread in mainland China. [ABSTRACT FROM AUTHOR]

Introduction and objectives: Implementation of a one-step strategy for diagnosis of active Hepatitis C virus (HCV) infection would encourage the early diagnosis and reduce the time to access antiviral treatments. The aim of this study was to evaluate the impact of a HCV one-step diagnosis compared to the traditional twostep protocol in terms of the time required for patients to be seen by specialists and the time taken to start antiviral treatment. Material and methods: A comparative study was carried out to assess two diagnostic algorithms (one-step and two-step) for active HCV infection. Serological markers were quantified using the same serum sample to determine both anti-HCV antibodies (HCV-Ab) and HCV core antigen (HCV-cAg) by Architect i2000 SR kit. In this period, a multidisciplinary procedure was started for telematics referral of viremic patients. Results: One-step approach reduced the time required for patient HCV diagnosis, referral to a specialist, access to treatment, and eliminated the loss of patients to follow-up. Significant differences were observed between one-step and two-step diagnosis methods in the time required for patients to be seen by a specialist (18 days [Interquartile range (IQR) = 14-42] versus 107 days [IQR = 62-148]) and for the initiation of treatment (54 days [IQR = 43-75] versus 200 days [IQR = 116-388]), mainly for patients with advanced fibrosis (35 days [IQR = 116-388] versus 126 days [IQR = 152-366]). Conclusions: Use of HCV-cAg has proven to be a useful tool for screening patients with active hepatitis C. The development of a multidisciplinary protocol for the communication of results improved the efficiency of the care process. [ABSTRACT FROM AUTHOR]

Abstract Gold nanoparticles (GNPs) have always been used as doxorubicin (DOX) transport vectors for tumor diagnosis and therapy; however, the synthesis process of these vectors is to prepare GNPs via chemical reduction method firstly, followed by conjugation with DOX or specific peptides, so these meth•ods faced some common problems including multiple steps, high cost, time consuming, complicated preparation, and post-processing. Here, we present a one-step strategy to prepare the DOX-conjugated GNPs on the basis of DOX’s chemical constitution for the first time. Moreover, we prepare a multifunctional GNPs (DRN-GNPs) with a one-step method by the aid of the reductive functional groups possessed by DOX, RGD peptides, and nuclear localization peptides (NLS), which only needs 30 min. The results of scattering images and cell TEM studies indicated that the DRN-GNPs could target the Hela cells’ nucleus. The tumor inhibition rates of DRN-GNPs via tumor and tail vein injection of nude mice were 66.7% and 57.7%, respectively, which were significantly enhanced compared to control groups. One step synthesis of multifunctional GNPs not only saves time, materials, but also it is in line with the development direction of green chemistry, and it would lay the foundation for large-scale applications within the near future. Our results suggested that the fabrication strategy is efficient, and our prepared DRN-GNPs possess good colloidal stability in the physiological system; they are a potentially contrast agent and an efficient DOX transport vector for cervical cancer diagnosis and therapy.

Porcine circovirus type 3 (PCV3), a novel circovirus, imposes great burdens on the global pig industry. The penside tests for detecting PCV3 are critical for assessing the epidemiological status and working out disease prevention and control programs due to the unavailability of a commercial vaccine. A one-step molecular assay based on visual loop-mediated isothermal amplification (vLAMP) was developed for simple and rapid detection of PCV3. We compared its sensitivity and specificity with TaqMan quantitative real-time polymerase chain reaction (qPCR) and applied the developed assay in the epidemiological study of (n = 407) pooled swine sera collected from almost the entire mainland China during the years 2017–2018. We also explored the feasibility of the vLAMP assay for detecting raw samples without a prior DNA isolation step to expand its application capability. Results showed that the vLAMP assay could reliably detect the PCV3 cap gene with a detection limit of 10 DNA copies equal to that of the Taqman qPCR assay. In the epidemiological study, the PCV3 positive detection rate for 407 swine pooled sera detected by the vLAMP assay was 37.35% (152/407), whereas it was 39.01% (159/407) for Taqman qPCR. For the detection method without genome extraction, the results kept satisfactory specificity (100%) but displayed lower sensitivity (100% for CT < 32), indicating the direct detection is not sensitive enough to discriminate the samples with low viral loads. The one-step vLAMP is a convenient, rapid, and cost-effective diagnostic for penside detection and will enable the epidemiological surveillance of PCV3, which has widely spread in mainland China.

Introduction and objectives: Implementation of a one-step strategy for diagnosis of active Hepatitis C virus (HCV) infection would encourage the early diagnosis and reduce the time to access antiviral treatments. The aim of this study was to evaluate the impact of a HCV one-step diagnosis compared to the traditional two-step protocol in terms of the time required for patients to be seen by specialists and the time taken to start antiviral treatment. Material and methods: A comparative study was carried out to assess two diagnostic algorithms (one-step and two-step) for active HCV infection. Serological markers were quantified using the same serum sample to determine both anti-HCV antibodies (HCV-Ab) and HCV core antigen (HCV-cAg) by Architect i2000 SR kit. In this period, a multidisciplinary procedure was started for telematics referral of viremic patients. Results: One-step approach reduced the time required for patient HCV diagnosis, referral to a specialist, access to treatment, and eliminated the loss of patients to follow-up. Significant differences were observed between one-step and two-step diagnosis methods in the time required for patients to be seen by a specialist (18 days [Interquartile range (IQR) = 14–42] versus 107 days [IQR = 62–148]) and for the initiation of treatment (54 days [IQR = 43–75] versus 200 days [IQR = 116–388]), mainly for patients with advanced fibrosis (35 days [IQR = 116–388] versus 126 days [IQR = 152–366]). Conclusions: Use of HCV-cAg has proven to be a useful tool for screening patients with active hepatitis C. The development of a multidisciplinary protocol for the communication of results improved the efficiency of the care process.

A novel difunctionalization of aryldiazonium salts was realized for the one-step generation of symmetric and asymmetric p-azophenols. This approach is proceeded by the sequentially regioselective aromatic C-O and C-N bond construction under mild reaction conditions, unlocking a new reaction strategy to facilitate the synthesis of p-azophenols.

Introduction: Mycoplasma pneumoniae (MP) is the major cause of respiratory infections that threaten the health of children and adolescents worldwide. Therefore, an early, simple, and accurate detection approach for MP is critical to prevent outbreaks of MP-induced community-acquired pneumonia., Methods: Here, we explored a simple and accurate method for MP identification that combines loop-mediated isothermal amplification (LAMP) with the CRISPR/Cas12b assay in a one-pot reaction., Results: In the current study, the whole reaction was completed within 1 h at a constant temperature of 57°C. The limit of detection of this assay was 33.7 copies per reaction. The specificity of the LAMP-CRISPR/Cas12b method was 100%, without any cross-reactivity with other pathogens. Overall, 272 clinical samples were used to evaluate the clinical performance of LAMP-CRISPR/Cas12b. Compared with the gold standard results from real-time PCR, the present method provided a sensitivity of 88.11% (126/143), specificity of 100% (129/129), and consistency of 93.75% (255/272)., Discussion: Taken together, our preliminary results illustrate that the LAMP-CRISPR/Cas12b method is a simple and reliable tool for MP diagnosis that can be performed in resource-limited regions., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Liu, Liu, Chen, Shi, Maimaiti, Yang, Zheng, Lu, Li and Chen.)

A visible light catalyzed three‐component tandem annulation strategy for the synthesis of multiply substituted 1,2,4‐triazole‐3,5‐diamines from phenylhydrazines, isothiocyanatobenzenes and 1,1,3,3‐tetramethylguanidine is reported herein. The transformations proceed under simple and mild reaction conditions without the requirement of any external metals, ligands, and reductants. This one‐step procedure provides facile access to highly substituted 1,2,4‐triazole‐3,5‐diamines, which could be used in the field of medicinal chemistry. [ABSTRACT FROM AUTHOR]

Nanoenergetics‐on‐a‐chip (NOC) have been widely used with excellent performance. Nevertheless, the development of simple, convenient, low cost, and environmentally friendly strategies for preparation of energetic metal‐organic framework (E‐MOF) NOC films remains a challenge. In this work, we present a one‐step solvothermal method to deposit three‐dimensional (3‐D) E‐MOF films, [MOFfilm(Cu); [Cu(atrz)3(NO3)2]n (atrz=4,4'‐azo‐1,2,4‐triazole)] on oxidized copper foil. Physicochemical properties, including morphologies, decomposition temperature, sensitivity, laser ignition characteristics, and flame height of the MOFfilm(Cu) films were analysed. With thermal stability up to 243 °C, the resulting MOFfilm(Cu) films exhibit low sensitivity (Impact sensitivity (IS): 16 J; Friction sensitivity (FS): 112 N, and Electrostatic sensitivity (ESD): 24.75 J), combined with laser ignition capability. After laser excitement, MOFfilm(Cu) films can act as an ignition material owing to a remarkable flame height (8 mm) and a violent explosion process (180 μs). More importantly, the energetic properties for MOFfilm(Cu) films are conveniently tunable via a simple anion exchange strategy, and two new energetic MOF films, MOFfilm(Cu) ⋅ SO4 and MOFfilm(Cu) ⋅ BF4, have been prepared. This work provides a simple, safe, convenient, and lower cost strategy for fabricating energetic MOF films that are applicable in NOC technologies, and opens a new field for the application of anion exchange strategy. [ABSTRACT FROM AUTHOR]