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2. Mutation of the imprinted gene OsEMF2a induces autonomous endosperm development and delayed cellularization in rice

Author

Tonosaki, Kaoru, Ono, Akemi, Kunisada, Megumi, Nishino, Megumi, Nagata, Hiroki, Sakamoto, Shingo, Kijima, Saku T, Furuumi, Hiroyasu, Nonomura, Ken-Ichi, Sato, Yutaka, Ohme-Takagi, Masaru, Endo, Masaki, Comai, Luca, Hatakeyama, Katsunori, Kawakatsu, Taiji, and Kinoshita, Tetsu

Subjects
Genetics, Human Genome, Endosperm, Epigenesis, Genetic, Gene Expression Regulation, Plant, Mutation, Oryza, Plant Proteins, Transcriptome, Biochemistry and Cell Biology, Plant Biology, Plant Biology & Botany
Abstract

In angiosperms, endosperm development comprises a series of developmental transitions controlled by genetic and epigenetic mechanisms that are initiated after double fertilization. Polycomb repressive complex 2 (PRC2) is a key component of these mechanisms that mediate histone H3 lysine 27 trimethylation (H3K27me3); the action of PRC2 is well described in Arabidopsis thaliana but remains uncertain in cereals. In this study, we demonstrate that mutation of the rice (Oryza sativa) gene EMBRYONIC FLOWER2a (OsEMF2a), encoding a zinc-finger containing component of PRC2, causes an autonomous endosperm phenotype involving proliferation of the central cell nuclei with separate cytoplasmic domains, even in the absence of fertilization. Detailed cytological and transcriptomic analyses revealed that the autonomous endosperm can produce storage compounds, starch granules, and protein bodies specific to the endosperm. These events have not been reported in Arabidopsis. After fertilization, we observed an abnormally delayed developmental transition in the endosperm. Transcriptome and H3K27me3 ChIP-seq analyses using endosperm from the emf2a mutant identified downstream targets of PRC2. These included >100 transcription factor genes such as type-I MADS-box genes, which are likely required for endosperm development. Our results demonstrate that OsEMF2a-containing PRC2 controls endosperm developmental programs before and after fertilization.

Published
2021

4. DNA demethylation by ROS1a in rice vegetative cells promotes methylation in sperm

Author

Kim, M Yvonne, Ono, Akemi, Scholten, Stefan, Kinoshita, Tetsu, Zilberman, Daniel, Okamoto, Takashi, and Fischer, Robert L

Subjects
Contraception/Reproduction, Genetics, Human Genome, Generic health relevance, Arabidopsis, DNA Glycosylases, DNA Methylation, DNA, Plant, Oryza, Ovule, Plant Development, Plant Proteins, Pollen, DNA methylation, DNA demethylation, epigenetics, rice, pollen
Abstract

Epigenetic reprogramming is required for proper regulation of gene expression in eukaryotic organisms. In Arabidopsis, active DNA demethylation is crucial for seed viability, pollen function, and successful reproduction. The DEMETER (DME) DNA glycosylase initiates localized DNA demethylation in vegetative and central cells, so-called companion cells that are adjacent to sperm and egg gametes, respectively. In rice, the central cell genome displays local DNA hypomethylation, suggesting that active DNA demethylation also occurs in rice; however, the enzyme responsible for this process is unknown. One candidate is the rice REPRESSOR OF SILENCING 1a (ROS1a) gene, which is related to DME and is essential for rice seed viability and pollen function. Here, we report genome-wide analyses of DNA methylation in wild-type and ros1a mutant sperm and vegetative cells. We find that the rice vegetative cell genome is locally hypomethylated compared with sperm by a process that requires ROS1a activity. We show that many ROS1a target sequences in the vegetative cell are hypomethylated in the rice central cell, suggesting that ROS1a also demethylates the central cell genome. Similar to Arabidopsis, we show that sperm non-CG methylation is indirectly promoted by DNA demethylation in the vegetative cell. These results reveal that DNA glycosylase-mediated DNA demethylation processes are conserved in Arabidopsis and rice, plant species that diverged 150 million years ago. Finally, although global non-CG methylation levels of sperm and egg differ, the maternal and paternal embryo genomes show similar non-CG methylation levels, suggesting that rice gamete genomes undergo dynamic DNA methylation reprogramming after cell fusion.

Published
2019

8. A surge in respiratory syncytial virus infection-related hospitalizations associated with the COVID-19 pandemic: An observational study at pediatric emergency referral hospitals in Tokushima Prefecture

Author

Shichijo, Koichi, primary, Fukura, Shoko, additional, Takeuchi, Shunsuke, additional, Tayama, Takahiro, additional, Ono, Akemi, additional, Ichihara, Yuko, additional, Suga, Kenichi, additional, Sato, Hiroki, additional, Takechi, Atsumi, additional, Matsumoto, Sonomi, additional, Fujino, Shuji, additional, Taniguchi, Takako, additional, Takahashi, Akiyoshi, additional, Watanabe, Tsutomu, additional, and Kondo, Shuji, additional

Published
2023
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13. IVA for RV function analysis in TOF

Author

Ono, Akemi, Hayabuchi, Yasunobu, Tanaka, Manami, and Kagami, Shoji

Subjects
pulmonary annulus, sense organs, tetralogy of Fallot, isovolumic acceleration, right ventriclar function
Abstract

Assessment of right ventricular (RV) function is quite important in patients with surgically corrected tetralogy of Fallot (TOF). However, quantitative assessment of RV function remains challenging, mainly because of the complex RV geometry. This prospective study investigated isovolumic acceleration (IVA), a parameter of myocardial systolic function not influenced by either preload or afterload, using tissue Doppler imaging. We evaluated IVA measured on pulmonary annulus (PA-IVA) and tricuspid annulus (TA-IVA), because we considered that PA-IVA and TA-IVA correspond with systolic function of the RV outflow tract (RVOT) and RV basal function, respectively. Thirty-nine patients with surgically repaired TOF (TOF group) and 40 age-matched healthy children (control group) were enrolled in this study. No significant difference was seen between TA-IVA (2.5 ± 0.8 m/s2) and PA-IVA (2.4 ± 0.8 m/s2) in the control group. In the TOF group, PA-IVA (1.0 ± 0.5 m/s2) was significantly lower than TA-IVA (1.3 ± 0.6 m/s2, p < 0.05). Both TA-IVA and PA-IVA were significantly lower in the TOF group than in the control group (p < 0.05 each). We concluded that PA-IVA offers a useful index to assess RVOT function in TOF patients.

Published
2020

17. Verification Using Healthy Adults of the Effects of Hydrogen Water Foot Baths

Author

Tamura, Sachiko, Yada, Naoyuki, Ono, Akemi, Tashiro, Keiko, and Nakabayashi, Makoto

Subjects
hydrogen water, effect, adult, foot bath
Abstract

application/pdf, To verify the effects of foot baths using hydrogen water (oxidation-reduction potential: -87 to -168 mV, hydrogen content: 0.1 to 0.2 ppm), seven healthy adult people were given foot baths using tap water and hydrogen water, and the results compared. The results show that when using the hydrogen water foot bath, there was a significant decrease in diastolic blood pressure coupled with a significant increase in the temperature of the dorsum pedis. This suggests that the effects of foot baths using hydrogen water are higher than those using tap water, and will improve peripheral blood flow.

Published
2019

23. Pulmonary annular motion velocity reflects right ventricular outflow tract function in children with surgically repaired congenital heart disease

Author

Hayabuchi, Yasunobu, Ono, Akemi, Homma, Yukako, and Kagami, Shoji

Subjects
Tissue Doppler imaging, cardiovascular system, Children, Right ventricular outflow tract, Congenital heart disease
Abstract

Right ventricular (RV) dysfunction is generally evaluated using analyses of tricuspid annular motion. However, it represents only one aspect of RV performance. Whether measuring pulmonary annular motion velocity could serve as a novel way to evaluate global RV and/or RV outflow tract (RVOT) performance in pediatric congenital heart disease (CHD) patients with surgically repaired RVOT was evaluated. In this prospective study, tissue Doppler-derived pulmonary annular motion velocity was measured in children (aged 2-5 years) with RVOT reconstruction (RVOTR group, n = 48) and age-matched healthy children (Control, n = 60). The types of RVOTR procedures were as follows: pulmonary valve-sparing procedure (PVS, n = 7); transannular patch with monocusp valve reconstruction (TAP, n = 29); and RV-to-PA conduit reconstruction using a pericardial valve with expanded polytetrafluoroethylene conduit (Rastelli, n = 12). Pulmonary annular motion velocity waveforms comprised systolic bimodal (s1’ and s2’) and diastolic e’ and a’ waves in all participants. The peak velocities of s1’, s2’, e’, and a’ were significantly lower in the RVOTR group than in the control group (all p < 0.0001). Furthermore, these parameters depended significantly on the type of surgical procedure. The peak velocities of s1’, s2’, and e’ had significant correlations with RVOT ejection fraction (RVOT-EF) (r = 0.56, 0.49, and 0.34, respectively) and RVOT fractional shortening (RVOT-FS) (r = 0.72, 0.55, and 0.41, respectively), although there were no significant correlations between pulmonary annular motion and global RV function, including RV ejection fraction (RVEF) and RV fractional area change (RVFAC) in the assessment of all RVOTR group patients. The pulmonary annular motion parameters in the PVS group had significant correlations with both global RV and RVOT performance. The TAP group showed significant correlations between RVOT function and pulmonary annular motion. The Rastelli group showed almost no significant correlations between RV/RVOT function and tissue Doppler parameters. Pulmonary annular motion velocity is a simple, rapid, reproducible, and useful method of assessing RVOT function in children with surgically repaired CHD.

Published
2017

24. Mutation of the imprinted geneOsEMF2ainduces autonomous endosperm development and delayed cellularization in rice

Author

Tonosaki, Kaoru, primary, Ono, Akemi, additional, Kunisada, Megumi, additional, Nishino, Megumi, additional, Nagata, Hiroki, additional, Sakamoto, Shingo, additional, Kijima, Saku T, additional, Furuumi, Hiroyasu, additional, Nonomura, Ken-Ichi, additional, Sato, Yutaka, additional, Ohme-Takagi, Masaru, additional, Endo, Masaki, additional, Comai, Luca, additional, Hatakeyama, Katsunori, additional, Kawakatsu, Taiji, additional, and Kinoshita, Tetsu, additional

Published
2020
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28. Next-generation sequencing for the diagnosis of patients with congenital multiple anomalies and / or intellectual disabilities

Author

Suga, Kenichi, primary, Imoto, Issei, additional, Ito, Hiromichi, additional, Naruto, Takuya, additional, Goji, Aya, additional, Osumi, Keita, additional, Tokaji, Narumi, additional, Homma, Yukako, additional, Ono, Akemi, additional, Ichihara, Yuko, additional, Shono, Miki, additional, Mori, Tatsuo, additional, Urushihara, Maki, additional, Nakagawa, Ryuji, additional, Hayabuchi, Yasunobu, additional, and Kagami, Shoji, additional

Published
2020
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40. Long-term Results After Open Mitral Commissurotomy for a One-Month-Old Infant With Mitral Stenosis

Author

Kitaichi, Takashi, primary, Sugano, Mikio, additional, Arase, Hiroki, additional, Kawatani, Yohei, additional, Kameta, Kanako, additional, Kurobe, Hirotsugu, additional, Fujimoto, Eiki, additional, Ono, Akemi, additional, Hayabuchi, Yasunobu, additional, Fujita, Hiroshi, additional, Sogabe, Hitoshi, additional, and Kitagawa, Tetsuya, additional

Published
2017
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45. Detection of 1p36 deletion by clinical exome-first diagnostic approach

Author

Watanabe, Miki, primary, Hayabuchi, Yasunobu, additional, Ono, Akemi, additional, Naruto, Takuya, additional, Horikawa, Hideaki, additional, Kohmoto, Tomohiro, additional, Masuda, Kiyoshi, additional, Nakagawa, Ryuji, additional, Ito, Hiromichi, additional, Kagami, Shoji, additional, and Imoto, Issei, additional

Published
2016
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