Following the intensive development of isolated (i.e., not coupled with on-chip waveguide) vertically rolled-up microtube ring resonators (VRU-MRRs) for both active and passive applications, a variety of microtube-based devices has been realized. These include microcavity lasers, optical sensors, directional couplers, and active elements in lab-on-a-chip devices. To provide more advanced and complex functionality, the focus of tubular geometry research is now shifting toward (i) refined vertical light transfer in 3D stacks of multiple photonic layers and (ii) to make microfluidic cooling system in the integrated optoelectronic system. Based on this motivation, this PhD research is devoted to the demonstration and the implementation of monolithic integration of VRU-MRRs with photonic waveguides for 3D photonic integration and their optofluidic applications. Prior to integration, high-quality isolated VRU-MRRs on the flat Si substrate are firstly fabricated by the controlled release of differentially strained titanium-dioxide (TiO2) bilayered nanomembranes. The fabricated microtubes support resonance modes for both telecom and visible photonics. The outcome of the isolated VRU-MRRs is a record high Q (≈3.8×10^3) in the telecom wavelength range with optimum tapered optical fiber resonator interaction. To further study the optical modes in the visible and near infrared spectral range, μPL spectroscopy is performed on the isolated VRU-MRRs, which are activated by entrapping various sizes of luminescent nanoparticles (NPs) within the windings of rolled-up nanomembranes based on a flexible, robust and economical method. Moreover, it is realized for the first time, in addition to serving as light sources that NPs-aggregated in isolated VRU-MRRs can produce an optical potential well that can be used to trap optical resonant modes. After achieving all the required parameters for creating a high-quality TiO2 VRU-MRR, the monolithic integration of VRU-MRRs with Si nanophotonic waveguides is experimentally demonstrated, exhibiting a significant step toward 3D photonic integration. The on-chip integration is realized by rolling up 2D pre-strained TiO2 nanomembranes into 3D VRU-MRRs on a microchip which seamlessly expanded over several integrated waveguides. In this intriguing vertical transmission configuration, resonant filtering of optical signals at telecom wavelengths is demonstrated based on ultra-smooth and subwavelength thick-walled VRU-MRRs. Finally, to illustrate the usefulness of the fully integrated VRU-MRRs with photonic waveguides, optofluidic functionalities of the integrated system is investigated. In this work, two methods are performed to explore optofluidic applications of the integrated system. First, the hollow core of an integrated VRU-MRR is uniquely filled with a liquid solution (purified water) by setting one end of the VRU-MRRs in contact with a droplet placed onto the photonic chip via a glass capillary. Second, the outside of an integrated VRU-MRR is fully covered with a big droplet of liquid. Both techniques lead to a significant shift in the WGMs (Δλ≈46 nm). A maximum sensitivity of 140 nm/refractive index unit, is achieved. The achievements of this PhD research open up fascinating opportunities for the realization of massively parallel optofluidic microsystems with more functionality and flexibility for analysis of biomaterials in lab-on-a-tube systems on single chips. It also demonstrates 3D photonic integration in which optical interconnects between multiple photonic layers are required.