1. Inflammatory profile of vertically HIV-1 infected adolescents receiving ART in Cameroon: a contribution toward optimal pediatric HIV control strategies
- Author
-
Aude Christelle Ka’e, Aubin Joseph Nanfack, Georgia Ambada, Maria Mercedes Santoro, Desire Takou, Ezechiel Ngoufack Jagni Semengue, Alex Durand Nka, Marie Laure Mpouel Bala, Orphelie Ndoh Endougou, Elise Elong, Grace Beloumou, Sandrine Djupsa, Davy Hyacinthe Gouissi, Nadine Fainguem, Michel Carlos Tommo Tchouaket, Samuel Martin Sosso, Daniel Kesseng, Francis Ateba Ndongo, Nelson Sonela, Arnaud Cedric Lacmago Kamta, Hyppolite K. Tchidjou, Therese Ndomgue, Suzie Tetang Moyo Ndiang, Anne Esther Njom Nlend, Celine Nguefeu Nkenfou, Carla Montesano, Gregory Edie Halle-Ekane, Giulia Cappelli, Caroline T. Tiemessen, Vittorio Colizzi, Francesca Ceccherini-Silberstein, Carlo-Federico Perno, and Joseph Fokam
- Subjects
HIV-1 ,inflammation ,antiretroviral therapy ,adolescents ,cytokines ,viral load ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Antiretroviral therapy (ART) has improved the lifespan of people living with HIV. However, their immune system remains in a state of sustained activation/inflammation, which favors viral replication and depletion of helper T-cells with varying profiles according to ART-response. We herein sought to ascertain the inflammatory profile of adolescents living with perinatal HIV-1 infection (ALPHI) receiving ART in an African context. In this cross-sectional and comparative study among ART-experienced ALPHI in Yaoundé-Cameroon, HIV-1 RNA was measured by Abbott Real-time PCR; CD4 cells were enumerated using flow cytometry; serum cytokines were measured by ELISA; HIV-1 proviral DNA was genotyped by Sanger-sequencing; and archived drug resistance mutations (ADRMs) were interpreted using Stanford HIVdb.v9.0.1. Overall, 73 adolescents were enrolled (60 ALPHI and 13 HIV-1 negative peers) aged 15 (13-18) years; 60.00% were female. ART median duration was 92 (46-123) months; median viral load was 3.99 (3.17-4.66) RNA Log10 (copies)/mL and median CD4 count was 326 (201-654) cells/mm3. As compared to HIV-negative adolescents, TNFα was highly expressed among ALPHI (p
- Published
- 2023
- Full Text
- View/download PDF