41 results on '"Ortega, Sheila"'
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2. Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
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Mohamed Echchakery, Carmen Chicharro, Samia Boussaa, Javier Nieto, Eugenia Carrillo, Ortega Sheila, Javier Moreno, and Ali Boumezzough
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Leishmania tropica ,L. infantum ,LnPCR ,ITS1 PCR ,Rodents ,Morocco ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasites’ transmission cycles. The present study investigates the role of rodent species as potential reservoirs of Leishmania spp. in central Morocco, where both L. tropica and L. infantum have been reported. Methods Rodents were caught from 22 sites in central Morocco, by using Sherman metal traps, and identified morphologically. For each specimen, genomic DNA was extracted from different tissues using the Speed Tools DNA extraction Kit. Then, samples were PCR-analyzed, targeting the SSU rRNA gene to detect Leishmania spp. DNA, followed by amplification of the internal transcribed spacer 1 (ITS1) and its sequencing to identify the species. Results A total of 197 rodents belonging to ten species were captured and identified: Rattus rattus (40.61%), Mus musculus (25.38%), Apodemus sylvaticus (8.63%), Mus spretus (7.11%), Meriones shawi (5.58%), Rattus norvegicus (4.57%), Meriones libycus (3.05%), Mastomys erythroleucus (2.03%), Gerbillus campestris (2.03%) and Lemniscomys barbarus (1.01%). Molecular analysis revealed the presence of Leishmania species in 18 specimens: six R. rattus (out of 80 captured; 7.5%), 11 M. musculus (out of 50 captured; 22%), and one R. norvegicus (out of 9 captured; 11.11%). Conclusions To the best of our knowledge, L. infantum and L. tropica were identified in rodent species for the first time in Morocco. These findings suggest that rodent species may be involved in L. infantum and L. tropica transmission cycles in this country but that further studies are needed to confirm their role as reservoirs of Leishmania species in Morocco.
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- 2017
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3. Viabilidade do home office para profissionais da área da tecnologia da informação: vantagens e desvantagens em adotar esse modelo de trabalho
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Mariano, Thais dos Santos, Carvalho, Denilson Luiz de, Ortega, Sheila, Mariano, Thais dos Santos, Carvalho, Denilson Luiz de, and Ortega, Sheila
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The emergence of the home office brought with it an opportunity for innovation and a new work model, differentiating from the usual. Since March 2020, many companies have chosen to use the remote format, due to the isolation restrictions imposed by the WHO (World Health Organization), due to the COVID-19 pandemic. This article aims to analyze the impacts caused on the productivity and motivation of the employee who is working in home-office. This is an exploratory survey, with a qualitative and quantitative approach and bibliographic design, in which a survey was applied via Google Forms to 61 professionals in the area of information technology. The main results attest that employees feel adapted and fit in remote work, in this way the home office is becoming a trend, among organizations, and may be the working style of the future., O surgimento do home office trouxe a oportunidade de inovação e um novo modelo de trabalho, se diferenciando do modo presencial, desde então muitas empresas começaram a implantar este formato remoto em seu ambiente corporativo, sobretudo com as pessoas do ramo de TI. Ao longo do tempo este método foi entrando em ascensão e obtendo uma grande procura, principalmente em meados de 2020, devido às restrições de isolamento social. O presente artigo tem como objetivo analisar os impactos causados na produtividade e motivação do colaborador que se encontra trabalhando em home-office. Trata-se de uma pesquisa exploratória, com abordagem qualitativa, quantitativa e delineamento bibliográfico, no qual foi aplicado um survey via Google Forms para 61 profissionais da área da tecnologia da informação. Os resultados principais atestam que os funcionários se sentem adaptados e aptos no trabalho remoto, desta forma o home office está se tornando uma tendência entre as organizações, podendo ser o estilo de trabalho do futuro., El surgimiento de home office trajo la oportunidad de la innovación y un nuevo modelo de trabajo, diferenciándose de lo habitual. Desde marzo de 2020, muchas empresas han optado por utilizar el formato remoto, debido a las restricciones de aislamiento impuestas por la OMS (Organización Mundial de la Salud), por la pandemia de COVID-19. El presente artículo tiene como objetivo analizar los impactos causados en la productividad y la motivación del empleado que se encuentra trabajando en home-office. Se trata de una investigación exploratoria, con enfoque cualitativo, cuantitativo y delineación bibliográfica, en la que se aplicó una encuesta a través de Google Forms a 61 profesionales del área de la tecnología de la información. Los principales resultados atestiguan que los empleados se sienten adaptados y aptos en el trabajo remoto, por lo que la oficina en casa se está convirtiendo en una tendencia entre las organizaciones, pudiendo ser el estilo de trabajo del futuro.
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- 2023
4. El discurso estudiantil en el día de la reapertura de la Universidad del Zulia
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Rincón Finol, Imelda, Ortega, Sheila, López, Gustavo, Rincón Finol, Imelda, Ortega, Sheila, and López, Gustavo
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This research work serves as a presentation of Number 41 of the Journal of the University of Zulia, a scientific publication that is part of the historical heritage of this university institution, which is why it is attached to the Free History Chair of the University of Zulia. The objective of the article is to identify the political vision of the speech delivered by Bachelor José Antonio Borjas, during the reopening of the University of Zulia, on October 1, 1946. The reopening of this university implied a whole historical process, whose background involves the time of the Hispanic colonization, extending until the foundation of the National College of Maracaibo, installed on April 19, 1837, being the most immediate antecedent for the creation and installation of the University of Zulia, on September 11, 1891. Among the The conclusions highlight the coincidence present in the student speech delivered by the bachelor José Antonio Borjas in the act of the Reopening of the University of Zulia, with the speech of the Rector Jesús Enrique Lossada, where both valued the reinstatement of the university as an achievement of the Revolution of October 18, 1945 in Venezuela., Este trabajo de investigación sirve de presentación del Número 41 de la Revista de la Universidad del Zulia, publicación científica que forma parte del patrimonio histórico de esta institución universitaria, por lo que está adscrita a la Cátedra Libre Historia de la Universidad del Zulia. El objetivo del artículo consiste en identificar la visión política del discurso pronunciado por el Bachiller José Antonio Borjas, durante la reapertura de la Universidad del Zulia, el 01 de Octubre de 1946. La reapertura de esta universidad implicó todo un proceso histórico, cuyos antecedentes involucra la época de la colonización hispánica, extendiéndose hasta la fundación del Colegio Nacional de Maracaibo, instalado el 19 de abril de 1837, siendo el antecedente más inmediato para la creación e instalación de la Universidad del Zulia, el 11 de Septiembre de 1891. Entre las conclusiones destaca la coincidencia presente en el discurso estudiantil pronunciado por el bachiller José Antonio Borjas en el acto de la Reapertura de la Universidad del Zulia, con el discurso del Rector Jesús Enrique Lossada, donde ambos valoraron la reinstalación de la universidad como un logro de la Revolución del 18 de Octubre de 1945 en Venezuela.
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- 2023
5. VIABILIDADE DO HOME OFFICE PARA PROFISSIONAIS DA ÁREA DA TECNOLOGIA DA INFORMAÇÃO: VANTAGENS E DESVANTAGENS EM ADOTAR ESSE MODELO DE TRABALHO.
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Ortega, Sheila Muramatsu, dos Santos Mariano, Thais, and Luiz de Carvalho, Denilson
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TELECOMMUTING ,INFORMATION technology - Abstract
Copyright of Revista Fatec Zona Sul (REFAS) is the property of Revista Fatec Zona Sul (REFAS) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2023
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6. Wild micromammal host spectrum of zoonotic eukaryotic parasites in Spain. Occurrence and genetic characterisation
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Vioque, Fátima, primary, Dashti, Alejandro, additional, Santín, Mónica, additional, Ruiz‐Fons, Francisco, additional, Köster, Pamela C., additional, Hernández‐Castro, Carolina, additional, García, Jesus T., additional, Bailo, Begoña, additional, Ortega, Sheila, additional, Olea, Pedro P., additional, Arce, Fernando, additional, Chicharro, Carmen, additional, Nieto, Javier, additional, González, Fernando, additional, Viñuela, Javier, additional, Carmena, David, additional, and González‐Barrio, David, additional
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- 2022
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7. Jesús Enrique Lossada: Maestro Rector de la reapertura de la Universidad del Zulia. A propósito del aniversario 75 de la Revista de la Universidad del Zulia
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Rincón, Imelda, primary, Ortega, Sheila, additional, and López, Gustavo, additional
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- 2022
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8. Zoonotic Enterocytozoon bieneusi genotypes in free-ranging and farmed wild ungulates in Spain
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Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Ayuntamiento de Barcelona, Ministerio de Ciencia e Innovación (España), Dashti, Alejandro, Santín, Mónica, Köster, Pamela C., Bailo, Begoña, Ortega, Sheila, Imaña, Elena, Habela, Miguel Ángel, Rivero-Juárez, Antonio, Vicente, Joaquín, Arnal, Maria Cruz, Fernández de Luco, Daniel, Morrondo, Patrocinio, Armenteros, José A., Balseiro, Ana, Cardona, Guillermo A., Martínez-Carrasco, Carlos, Ortiz, José-Antonio, Calero Bernal, Rafael, Carmena, David, González-Barrio, David, WE&H group, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Ayuntamiento de Barcelona, Ministerio de Ciencia e Innovación (España), Dashti, Alejandro, Santín, Mónica, Köster, Pamela C., Bailo, Begoña, Ortega, Sheila, Imaña, Elena, Habela, Miguel Ángel, Rivero-Juárez, Antonio, Vicente, Joaquín, Arnal, Maria Cruz, Fernández de Luco, Daniel, Morrondo, Patrocinio, Armenteros, José A., Balseiro, Ana, Cardona, Guillermo A., Martínez-Carrasco, Carlos, Ortiz, José-Antonio, Calero Bernal, Rafael, Carmena, David, González-Barrio, David, and WE&H group
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Microsporidia comprises a diverse group of obligate, intracellular, and spore-forming parasites that infect a wide range of animals. Among them, Enterocytozoon bieneusi is the most frequently reported species in humans and other mammals and birds. Data on the epidemiology of E. bieneusi in wildlife are limited. Hence, E. bieneusi was investigated in eight wild ungulate species present in Spain (genera Ammotragus, Capra, Capreolus, Cervus, Dama, Ovis, Rupicapra, and Sus) by molecular methods. Faecal samples were collected from free-ranging (n = 1058) and farmed (n = 324) wild ungulates from five Spanish bioregions. The parasite was detected only in red deer (10.4%, 68/653) and wild boar (0.8%, 3/359). Enterocytozoon bieneusi infections were more common in farmed (19.4%, 63/324) than in wild (1.5%, 5/329) red deer. A total of 11 genotypes were identified in red deer, eight known (BEB6, BEB17, EbCar2, HLJD-V, MWC_d1, S5, Type IV, and Wildboar3) and three novel (DeerSpEb1, DeerSpEb2, and DeerSpEb3) genotypes. Mixed genotype infections were detected in 15.9% of farmed red deer. Two genotypes were identified in wild boar, a known (Wildboar3) and a novel (WildboarSpEb1) genotypes. All genotypes identified belonged to E. bieneusi zoonotic Groups 1 and 2. This study provides the most comprehensive epidemiological study of E. bieneusi in Spanish ungulates to date, representing the first evidence of the parasite in wild red deer populations worldwide. Spanish wild boars and red deer are reservoir of zoonotic genotypes of E. bieneusi and might play an underestimated role in the transmission of this microsporidian species to humans and other animals.
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- 2022
9. Wild micromammal host spectrum of zoonotic eukaryotic parasites in Spain. Occurrence and genetic characterisation
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Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España), Fundación BBVA, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Instituto de Salud Carlos III, Universidad Autónoma de Madrid, Vioque, Fátima, Dashti, Alejandro, Santín, Mónica, Ruiz-Fons, Francisco, Köster, Pamela C., Hernández-Castro, Carolina, García, Jesús T., Bailo, Begoña, Ortega, Sheila, Olea, Pedro P., Arce, Fernando, Chicharro, Carmen, Nieto, Javier, González, Fernando, Viñuela, Javier, Carmena, David, González-Barrio, David, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España), Fundación BBVA, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Instituto de Salud Carlos III, Universidad Autónoma de Madrid, Vioque, Fátima, Dashti, Alejandro, Santín, Mónica, Ruiz-Fons, Francisco, Köster, Pamela C., Hernández-Castro, Carolina, García, Jesús T., Bailo, Begoña, Ortega, Sheila, Olea, Pedro P., Arce, Fernando, Chicharro, Carmen, Nieto, Javier, González, Fernando, Viñuela, Javier, Carmena, David, and González-Barrio, David
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Micromammals have historically been recognized as highly contentious species in terms of the maintenance and transmission of zoonotic pathogens to humans. Limited information is currently available on the epidemiology and potential public health significance of intestinal eukaryotes in wild micromammals. We examined 490 faecal samples, grouped into 155 pools, obtained from 11 micromammal species captured in 11 Spanish provinces for the presence of DNA from Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. The presence of Leishmania spp. was investigated in individual spleen samples. All micromammal species investigated harboured infections by at least one eukaryotic parasite, except Apodemus flavicollis, Myodes glareolus, Sorex coronatus and Sciurus vulgaris, but the sample size for these host species was very low. Cryptosporidium spp. was the most prevalent species found (3.7%, 95% confidence interval [CI]: 2.2–5.7), followed by G. duodenalis (2.8%, 95% CI: 1.6–4.6) and E. bieneusi (2.6%, 95% CI: 1.4–4.3). All pooled faecal samples tested negative for Blastocystis sp. Leishmania infantum was identified in 0.41% (95% CI: 0.05–1.46) of the 490 individual spleen samples analysed. Sequence analyses allowed the identification of Cryptosporidium andersoni (5.9%), C. ditrichi (11.7%), C. muris (5.9%), C. parvum (5.9%), C. tyzzeri (5.9%), rat genotypes CR97 (5.9%) and W19 (5.9%), vole genotypes V (11.7%) and VII (5.9%) and Cryptosproridium spp. (35.3%) within Cryptosporidium (n = 17). Known genotypes C (66.7%) and Peru11 (25.0%) and a novel genotype (named MouseSpEb1, 8.3%) were detected within E. bieneusi (n = 12). None of the G. duodenalis-positive samples could be genotyped at the assemblage level. Molecular data indicate that wild micromammals were primarily infected by rodent-adapted species/genotypes of eukaryotic pathogens and thereby have a limited role as a source of human infections. The presence of ruminant-adapted species C.
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- 2022
10. Parental leave in medical school: supporting students as parents
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Ortega, Sheila R., primary, Barnes, Jacob M., additional, and Waller, Jacquelyn D., additional
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- 2022
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11. Zoonotic Enterocytozoon bieneusi genotypes in free-ranging and farmed wild ungulates in Spain
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Dashti, Alejandro, Santín, Mónica, Köster, Pamela C, Bailo, Begoña, Ortega, Sheila, Imaña, Elena, Habela, Miguel Ángel, Rivero-Juarez, Antonio, Vicente, Joaquin, Conejero, Carles, González-Crespo, Carlos, Garrido, Cristina, Gassó, Diana, Andrea Murillo, Diana, Serrano, Emmanuel, Mentaberre, Gregorio, Torres-Blas, Irene, Estruch, Josep, Pastor, Josep, Ramón López-Olvera, Jorge, Escobar-González, María, Valldeperes, Marta, Mesalles, Montse, López, Omar, Álvarez, Raquel, Cuenca, Rafaela, Velarde, Roser, Lavín, Santiago, Arnal, Maria C, Fernánez de Luco, Daniel, Morrondo, Patrocinio, Armenteros, José A, Balseiro, Ana, Cardona, Guillermo A, Martínez-Carrasco, Carlos, Ortiz, José Antonio, Calero-Bernal, Rafael, Carmena, David, González-Barrio, David, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Ayuntamiento de Barcelona, and Ministerio de Ciencia e Innovación (España)
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Swine Diseases ,molecular diversity ,China ,Sheep ,Enterocytozoon bieneusi ,Genotype ,Swine ,Deer ,Sus scrofa ,Sheep Diseases ,Animals, Wild ,General Medicine ,Enterocytozoon ,Feces ,Infectious Diseases ,Spain ,Microsporidiosis ,wild ungulates ,Prevalence ,Animals ,Humans ,Phylogeny - Abstract
WE&H group: Carles Conejero, Carlos González-Crespo, Cristina Garrido, Diana Gassó, Diana Andrea Murillo, Emmanuel Serrano, Gregorio Mentaberre, Irene Torres-Blas, Josep Estruch, Josep Pastor, Jorge Ramón López-Olvera, María Escobar-González, Marta Valldeperes, Montse Mesalles, Omar López, Raquel Álvarez, Rafaela Cuenca, Roser Velarde, Santiago Lavín., Microsporidia comprises a diverse group of obligate, intracellular, and spore-forming parasites that infect a wide range of animals. Among them, Enterocytozoon bieneusi is the most frequently reported species in humans and other mammals and birds. Data on the epidemiology of E. bieneusi in wildlife are limited. Hence, E. bieneusi was investigated in eight wild ungulate species present in Spain (genera Ammotragus, Capra, Capreolus, Cervus, Dama, Ovis, Rupicapra, and Sus) by molecular methods. Faecal samples were collected from free-ranging (n = 1058) and farmed (n = 324) wild ungulates from five Spanish bioregions. The parasite was detected only in red deer (10.4%, 68/653) and wild boar (0.8%, 3/359). Enterocytozoon bieneusi infections were more common in farmed (19.4%, 63/324) than in wild (1.5%, 5/329) red deer. A total of 11 genotypes were identified in red deer, eight known (BEB6, BEB17, EbCar2, HLJD-V, MWC_d1, S5, Type IV, and Wildboar3) and three novel (DeerSpEb1, DeerSpEb2, and DeerSpEb3) genotypes. Mixed genotype infections were detected in 15.9% of farmed red deer. Two genotypes were identified in wild boar, a known (Wildboar3) and a novel (WildboarSpEb1) genotypes. All genotypes identified belonged to E. bieneusi zoonotic Groups 1 and 2. This study provides the most comprehensive epidemiological study of E. bieneusi in Spanish ungulates to date, representing the first evidence of the parasite in wild red deer populations worldwide. Spanish wild boars and red deer are reservoir of zoonotic genotypes of E. bieneusi and might play an underestimated role in the transmission of this microsporidian species to humans and other animals., This work was supported by Health Institute Carlos III (ISCIII), Spanish Ministry of Economy and Competitiveness, under project PI19CIII/00 029. Sample collection in Catalonia was supported by the Council of Barcelona through contracts 13/051, 15/0174, 16/0243 and 16/0243-00-PR/01, from the Spanish Ministry of Economy and Competitiveness under research grants CGL2012- 40043-C02-01, CGL2012-40043-C02-02, and from the Spanish Ministry of Science and Innovation under research grant CGL2016-80543-P PID2020-115046GB-I00, and USDA-ARS Project No: 8042 -32000-112 -00-D., Antonio Rivero-Juárez is the recipient of a Miguel Servet Research Contract by the Ministerio de Ciencia, Promoción y Universidades of Spain (CP18/00111). D.G.B. is the recipient of a Sara Borrell Research Contract (CD19CIII/00011) funded by the Spanish Ministry of Science, Innovation and Universities. A.D. is the recipient of a PFIS contract (FI20CIII/00002) funded by the Spanish Ministry of Science and Innovation and Universities., Medical Mycology
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- 2022
12. Diarrhoea‐causing enteric protist species in intensively and extensively raised pigs ( Sus scrofa domesticus ) in Southern Spain. Part II: Association with Hepatitis E virus susceptibility
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Rivero‐Juarez, Antonio, primary, Dashti, Alejandro, additional, Santín, Mónica, additional, Köster, Pamela C., additional, Lopez‐Lopez, Pedro, additional, Risalde, María A., additional, García‐Bocanegra, Ignacio, additional, Gomez‐Villamandos, Jose Carlos, additional, Caballero‐Gómez, Javier, additional, Frías, Mario, additional, Bailo, Begoña, additional, Ortega, Sheila, additional, Muadica, Aly Salimo, additional, Calero‐Bernal, Rafael, additional, González‐Barrio, David, additional, Rivero, Antonio, additional, Briz, Verónica, additional, and Carmena, David, additional
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- 2021
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13. Diarrhoea‐causing enteric protist species in intensively and extensively raised pigs ( Sus scrofa domesticus ) in Southern Spain. Part I: Prevalence and genetic diversity
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Dashti, Alejandro, primary, Rivero‐Juárez, Antonio, additional, Santín, Mónica, additional, George, Nadja S., additional, Köster, Pamela C., additional, López‐López, Pedro, additional, Risalde, María A, additional, García‐Bocanegra, Ignacio, additional, Gómez‐Villamandos, Jose Carlos, additional, Caballero‐Gómez, Javier, additional, Frías, Mario, additional, Bailo, Begoña, additional, Ortega, Sheila, additional, Muadica, Aly Salimo, additional, Calero‐Bernal, Rafael, additional, González‐Barrio, David, additional, Rivero, Antonio, additional, Briz, Verónica, additional, and Carmena, David, additional
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- 2021
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14. Molecular Detection and Characterization of Blastocystis sp. and Enterocytozoon bieneusi in Cattle in Northern Spain
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Abarca, Nadia, primary, Santín, Mónica, additional, Ortega, Sheila, additional, Maloney, Jenny G., additional, George, Nadja S., additional, Molokin, Aleksey, additional, Cardona, Guillermo A., additional, Dashti, Alejandro, additional, Köster, Pamela C., additional, Bailo, Begoña, additional, Hernández-de-Mingo, Marta, additional, Muadica, Aly S., additional, Calero-Bernal, Rafael, additional, Carmena, David, additional, and González-Barrio, David, additional
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- 2021
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15. Determinación de contaminantes orgánicos en muestras agroalimentarias mediante técnicas miniaturizadas de extracción combinadas con técnicas de separación acopladas a espectrometría de masas
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Mejías Ortega, Sheila, Rodríguez Delgado, Miguel Ángel, Socas Rodríguez, Bárbara, Máster Universitario en Seguridad y Calidad de los Alimentos, and Socas Rodriguez, Bárbara
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En este trabajo se propone una metodología analítica basada en el método QuEChERS y la cromatografía de gases acoplada a un espectrómetro de masas para la determinación de un grupo de disruptores endocrinos, incluyendo bisfenoles y alquilfenoles, en muestras de zumos. Abstract. In this work, an analytical methodology based on QuEChERS method and gas chromatography tandem mass spectrometry is proposed for the determination of endocrine disruptors, including bisphenols and alkylphenols, in juice samples.
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- 2021
16. Diarrhoea‐causing enteric protist species in intensively and extensively raised pigs (Sus scrofa domesticus) in Southern Spain. Part II: Association with Hepatitis E virus susceptibility.
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Rivero‐Juárez, Antonio, Dashti, Alejandro, Santín, Mónica, Köster, Pamela C., López‐López, Pedro, Risalde, María A., García‐Bocanegra, Ignacio, Gómez‐Villamandos, José Carlos, Caballero‐Gómez, Javier, Frías, Mario, Bailo, Begoña, Ortega, Sheila, Muadica, Aly Salimo, Calero‐Bernal, Rafael, González‐Barrio, David, Rivero, Antonio, Briz, Verónica, and Carmena, David
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CRYPTOSPORIDIUM ,HEPATITIS E virus ,SWINE ,SPECIES ,ANIMAL populations ,FOOD pathogens - Abstract
Enteropathogenic parasites can infect a wide range of mammals, including humans, supposing an important zoonotic risk. Hepatitis E virus (HEV) is an emerging foodborne pathogen of increasing public health relevance, affecting both humans and animal populations. Because both microorganisms share faecal‐oral transmission route they may constitute an excellent model to evaluate the interplay between them. Thus, we aim to evaluate the viral‐parasite interactions at the enteric interface in swine. We included pigs of two different breeds farming in South Spain under different production systems. We compared the HEV prevalence by the presence of Giardia duodenalis, Cryptosporidium spp., Balantioides coli, Blastocystis sp. and Enterocytozoon bieneusi in faecal samples. The HEV prevalence was 13.1 (62 out 475, 95% CI: 10.2–16.4). Those pigs infected with Cryptosporidium spp. showed a higher prevalence of HEV (30.8 vs. 12%; p =.012). In the same way, animals bearing E. bieneusi seem to have a higher rate of HEV infection (24.2 vs. 12.2%; p =.06). According to their location in the gut, animals bearing intracellular enteroparasites showed a higher HEV prevalence than those uninfected (29.6 vs. 12.7%; p =.038), meanwhile those carrying extracellular enteroparasites had a lower likelihood to be infected by HEV than those uninfected (12.1 vs. 23.1%; p =.071). Those animals bearing both types of enteroparasites showed a similar prevalence of HEV infection than those exhibiting negative for both (20.8 vs. 26.1%; p =.763). Our study provides evidence that intracellular and extracellular enteroparasites modulate the susceptibility to HEV infection in pigs. Meanwhile, the presence of extracellular enteroparasites shows a protective effect on the risk of HEV acquisition in swine, whereas intracellular enteroparasites seems to have the opposite effect, favouring the HEV infection. [ABSTRACT FROM AUTHOR]
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- 2022
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17. Diarrhoea‐causing enteric protist species in intensively and extensively raised pigs (Sus scrofa domesticus) in Southern Spain. Part I: Prevalence and genetic diversity.
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Dashti, Alejandro, Rivero‐Juárez, Antonio, Santín, Mónica, George, Nadja S., Köster, Pamela C., López‐López, Pedro, Risalde, María A, García‐Bocanegra, Ignacio, Gómez‐Villamandos, Jose Carlos, Caballero‐Gómez, Javier, Frías, Mario, Bailo, Begoña, Ortega, Sheila, Muadica, Aly Salimo, Calero‐Bernal, Rafael, González‐Barrio, David, Rivero, Antonio, Briz, Verónica, and Carmena, David
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CRYPTOSPORIDIUM ,SWINE ,GENETIC variation ,SPECIES - Abstract
Numerous protist species are shared between humans and pigs. Among those, Giardia duodenalis, Cryptosporidium spp. and Balantioides coli have a clear public and animal health significance. For others such as Enterocytozoon bieneusi and Blastocystis sp., their impact on animal health has not been fully established. Little information is currently available on the molecular diversity of these protists in swine populations. To fill this gap, we molecularly assessed G. duodenalis, Cryptosporidium spp., B. coli, Blastocystis sp. and E. bieneusi in faecal samples from Iberian and Large White pigs raised under different (intensive and/or extensive) management systems in southern Spain. A total of 151 extensively raised Iberian pigs, 140 intensively raised Iberian pigs, and 184 intensively raised Large White pigs were investigated. Blastocystis sp. was the agent most prevalently found (47.8%), followed by B. coli (45.5%), G. duodenalis (10.7%), E. bieneusi (6.9%), and Cryptosporidium spp. (5.5%). Blastocystis sp. was significantly less prevalent in intensively raised Iberian pigs (22.9%) than in their extensively raised counterparts (51.0%) or in intensively raised Large White pigs (64.1%). A significantly higher prevalence was found for G. duodenalis, Cryptosporidium spp., and E. bieneusi in Large White pigs than Iberian pigs. Balantioides coli was similarly distributed (40.0–51.1%) in all three investigated swine populations. Sequence analyses revealed the presence of G. duodenalis assemblage E, two Cryptosporidium species (Cryptosporidium scrofarum and Cryptosporidium suis), B. coli (genotypes A and B), Blastocystis sp. (ST1, ST3, and ST5), and E. bieneusi (EbpA, EbpC, EbpD, O, and a novel genotype named PigSpEb2). Novel genotype PigSpEb2 was found alone or in combination with EbpA. Data suggest a widespread exposure to protist enteroparasites in domestic pig populations irrespectively of breed and raising management system. Many of the species/genotypes identified have a zoonotic potential and might represent a public health concern. [ABSTRACT FROM AUTHOR]
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- 2022
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18. Loop-Mediated Isothermal Amplification Allows Rapid, Simple and Accurate Molecular Diagnosis of Human Cutaneous and Visceral Leishmaniasis Caused by Leishmania infantum When Compared to PCR
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Ibarra-Meneses, Ana Victoria, primary, Chicharro, Carmen, additional, Sánchez, Carmen, additional, García, Emilia, additional, Ortega, Sheila, additional, Ndung’u, Joseph Mathu, additional, Moreno, Javier, additional, Cruz, Israel, additional, and Carrillo, Eugenia, additional
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- 2021
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19. Importancia de la tecnología ambiental para el gerente empresarial / The Importance of Environmental Technology for the Company Manager
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Ramos, César, Plata, Dalia, Ortega, Sheila, García, Jesús, Ramos, César, Plata, Dalia, Ortega, Sheila, and García, Jesús
- Abstract
ResumenEste estudio en proceso de construcción, tiene como objetivo de terminar la importancia de la Tecnología Ambiental para el gerente empresarial. Esta investigación es de tipo descriptivo donde se hizo un análisis documental a la información recopilada. Resultados preliminares: a) las legislaciones ambientales obligan la incorporación de la tecnología ambiental en los procesos de producción industrial y en la obtención de productos biodegradables y b) la tecnología ambiental permite al gerente empresarial tener organizaciones altamente competitivas con tecnología de avanzada sin deteriorar el ambiente. En conclusión, la tecnología ambiental en el sector productivo, propicia que el gerente empresarial pueda ser asertivo en la selección de la alta tecnología en la optimización de su negocio con margen mínimo de impacto ambiental.AbstractThis study in process has the objective of determining the importance ofenvironmental technology for the company manager. The research is of thedescriptive type using documentary analysis of the collected information.Preliminary results: a) environmental legislation requires the incorporation ofenvironmental technology in industrial production processes and in obtainingbiodegradable products; b) environmental technology allows the companymanager to have a highly competitive organization with advanced technologywithout deteriorating the environment. In conclusion, environmentaltechnology in the productive sector makes it possible for the company managerto be assertive in selecting high technology for optimizing his business with aminimum margin of environmental impact
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- 2020
20. Prevalence of asymptomatic Leishmania infection and associated risk factors, after an outbreak in the south-western Madrid region, Spain, 2015
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Ibarra-Meneses, Ana Victoria, primary, Carrillo, Eugenia, additional, Nieto, Javier, additional, Sánchez, Carmen, additional, Ortega, Sheila, additional, Estirado, Alicia, additional, Latasa Zamalloa, Pello, additional, Sanz, Juan Carlos, additional, García-Comas, Luis, additional, Ordobás, María, additional, and Moreno, Javier, additional
- Published
- 2019
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21. Cellular Markers of Active Disease and Cure in Different Forms of Leishmania infantum-Induced Disease
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Botana, Laura, primary, Matía, Belén, additional, San Martin, Juan V., additional, Romero-Maté, Alberto, additional, Castro, Alicia, additional, Molina, Laura, additional, Fernandez, Laura, additional, Ibarra-Meneses, Ana, additional, Aguado, Marta, additional, Sánchez, Carmen, additional, Horrillo, Luis, additional, Chicharro, Carmen, additional, Nieto, Javier, additional, Ortega, Sheila, additional, Ruiz-Giardin, José Manuel, additional, Carrillo, Eugenia, additional, and Moreno, Javier, additional
- Published
- 2018
- Full Text
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22. Vigencia de la reforma universitaria de Córdoba en la gestión pública
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Rincón, Imelda, Ortega, Sheila, Rincón, Imelda, and Ortega, Sheila
- Abstract
Este estudio tiene como propósito analizar la vigencia histórica de la reforma universitaria de Córdoba en la gestión pública regional. Para ello, se desarrolló una metodología descriptiva-explicativa y proyectiva con diseño documental relacionados al tema antes señalado. Esto arrojó como resultado la participación y el protagonismo de estudiantes, intelectuales, trabajadores en general que trascendieron el ámbito nacional de Argentina, sintetizado en el Manifiesto Laminar denominado “El grito de Córdoba”, con el pliego de 10 peticiones. Se concluyó que la misión específica de las universidades debe ser generar educación, ciencia y tecnología capaz de innovar el contexto sociopolítico en función de la igualdad y la justicia social; por lo que se recomiendan lineamientos estratégicos de interacción orgánica entre las universidades y la gestión pública regional orientados para el esclarecimiento y soluciones concretas de los problemas medulares de la educación regional, con el fin de concretar en acciones reales, la igualdad y la justicia social reglamentadas en las políticas públicas.
- Published
- 2018
23. Additional file 1: Figure S1. of Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
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Echchakery, Mohamed, Chicharro, Carmen, Boussaa, Samia, Nieto, Javier, Carrillo, Eugenia, Ortega Sheila, Moreno, Javier, and Boumezzough, Ali
- Abstract
Profile of agarose (1.5%) gel for diagnostic of Leishmania spp. in DNA extracted from tissue from rodent species, using LnPCR for to amplify part of the SSU rRNA gene for diagnosis. Lane MM: 100 bp molecular marker (DNA ladder); Lanes 1, 12: negative controls (no DNA PCR); Lane 13: positive control (MHOM/FR/78/LEM75); Lanes 2–11: DNA extracted of tissue from rodent species. LnPCR for first amplification was carried out using R221/R332 primers. Figure S2. Profile of agarose (1.5%) gel for diagnostic of Leishmania spp. in DNA extracted from tissue from rodent species, using LnPCR for to amplify part of the SSU rRNA gene for diagnosis. Lane MM: 100 bp molecular marker (DNA ladder); Lanes 1, 12, 14: negative controls (no DNA PCR); Lanes 13, 15: positive controls (MHOM/FR/78/LEM75); Lanes 2–11: DNA extracted of tissue from rodent species. LnPCR for second amplification was carried out using R233/R333 primers. Figure S3. Profile of agarose (1.5%) gel for characterization of Leishmania species in DNA extracted from tissue from rodent species, using ITS1-PCR for to amplify the ribosomal internal transcribed spacer 1 (ITS1) region. Lane MM: 100 bp molecular marker (DNA ladder); Lanes 1, 16, 18: negative controls (no DNA PCR); Lanes 17, 19: positive controls (MHOM/FR/78/LEM75); Lanes 2–15: DNA extracted of tissue from rodent species. ITS1-PCR for second amplification was carried out using SAC/VAN2 primers. (DOCX 314 kb)
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- 2017
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24. Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
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Javier Nieto, Carmen Chicharro, Ali Boumezzough, Ortega Sheila, Mohamed Echchakery, Javier Moreno, Samia Boussaa, and Eugenia Carrillo
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0301 basic medicine ,Male ,Veterinary medicine ,Leishmania tropica ,Endemic Diseases ,Mus spretus ,030231 tropical medicine ,ITS1 PCR ,Leishmaniasis, Cutaneous ,Rodentia ,Lemniscomys barbarus ,Rodents ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Cutaneous leishmaniasis ,parasitic diseases ,medicine ,Animals ,Humans ,lcsh:RC109-216 ,Leishmania infantum ,Disease Reservoirs ,Meriones shawi ,biology ,LnPCR ,Research ,Leishmaniasis ,030108 mycology & parasitology ,DNA, Protozoan ,biology.organism_classification ,Leishmania ,medicine.disease ,L. infantum ,Rats ,Morocco ,Infectious Diseases ,Leishmaniasis, Visceral ,Parasitology ,Female ,Gerbillinae - Abstract
Background Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasites’ transmission cycles. The present study investigates the role of rodent species as potential reservoirs of Leishmania spp. in central Morocco, where both L. tropica and L. infantum have been reported. Methods Rodents were caught from 22 sites in central Morocco, by using Sherman metal traps, and identified morphologically. For each specimen, genomic DNA was extracted from different tissues using the Speed Tools DNA extraction Kit. Then, samples were PCR-analyzed, targeting the SSU rRNA gene to detect Leishmania spp. DNA, followed by amplification of the internal transcribed spacer 1 (ITS1) and its sequencing to identify the species. Results A total of 197 rodents belonging to ten species were captured and identified: Rattus rattus (40.61%), Mus musculus (25.38%), Apodemus sylvaticus (8.63%), Mus spretus (7.11%), Meriones shawi (5.58%), Rattus norvegicus (4.57%), Meriones libycus (3.05%), Mastomys erythroleucus (2.03%), Gerbillus campestris (2.03%) and Lemniscomys barbarus (1.01%). Molecular analysis revealed the presence of Leishmania species in 18 specimens: six R. rattus (out of 80 captured; 7.5%), 11 M. musculus (out of 50 captured; 22%), and one R. norvegicus (out of 9 captured; 11.11%). Conclusions To the best of our knowledge, L. infantum and L. tropica were identified in rodent species for the first time in Morocco. These findings suggest that rodent species may be involved in L. infantum and L. tropica transmission cycles in this country but that further studies are needed to confirm their role as reservoirs of Leishmania species in Morocco. Electronic supplementary material The online version of this article (10.1186/s13071-017-2398-8) contains supplementary material, which is available to authorized users.
- Published
- 2017
25. Validation of rK39 immunochromatographic test and direct agglutination test for the diagnosis of Mediterranean visceral leishmaniasis in Spain
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Bangert, Mathieu, primary, Flores-Chávez, María D., additional, Llanes-Acevedo, Ivonne P., additional, Arcones, Carolina, additional, Chicharro, Carmen, additional, García, Emilia, additional, Ortega, Sheila, additional, Nieto, Javier, additional, and Cruz, Israel, additional
- Published
- 2018
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26. Aplicaciones biomédicas de nanopartículas magnéticas
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Ortega, Sheila and Torrecillas, Ramón
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education - Published
- 2016
27. Prevalence of asymptomatic Leishmania infection and associated risk factors, after an outbreak in the southwestern Madrid region, Spain, 2015.
- Author
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Ibarra-Meneses, Ana Victoria, Carrillo, Eugenia, Nieto, Javier, Sánchez, Carmen, Ortega, Sheila, Estirado, Alicia, Zamalloa, Pello Latasa, Sanz, Juan Carlos, García-Comas, Luis, Ordobás, María, and Moreno, Javier
- Published
- 2019
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28. Aplicaciones biomédicas de nanopartículas magnéticas
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Torrecillas, Ramón, Ortega, Sheila, Torrecillas, Ramón, and Ortega, Sheila
- Published
- 2016
29. PROPUESTA DE LINEAMIENTOS GEOESTRATÉGICOS PARA EL DESARROLLO EDUCATIVO DE LA ZONA FRONTERIZA: MUNICIPIO PÁEZ DEL ESTADO ZULIA
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Marcano, Noraida and Ortega, Sheila
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lcsh:LC8-6691 ,lcsh:Special aspects of education ,Lineamientos geoestratégicos para el desarrollo educativo ,Geostrategic outlines for the educational development ,Border area ,Páez Municipality of the Zulia state ,lcsh:L ,Zona fronteriza ,Municipio Páez del Estado Zulia ,lcsh:Education - Abstract
El trabajo que se presenta tiene como finalidad proponer algunos Lineamientos Geoestratégicos para el Desarrollo Educativo de la Zona Fronteriza, Municipio Páez del Estado Zulia, los cuales derivan del análisis de los factores que condicionan el desarrollo educativo de ese municipio, donde se identifican los aspectos educativos, económicos, socioculturales, demográficos y físico-naturales de esa realidad fronteriza a través de la metodología descriptiva-exploratoria. Se trabajó con dos muestras estratificadas, una del personal docente de las instituciones educativas ubicadas en la zona y la otra, conformada por los padres y representantes de los estudiantes cursantes en estas instituciones. Las técnicas e instrumentos utilizados fueron la observación directa de los elementos intervinientes y la encuesta tipo cuestionario, las cuales permitieron obtener los insumos necesarios para determinar que esa zona fronteriza requiere una propuesta geoestratégica, con visión prospectiva que, apoyada en un enfoque sistémico y holístico, conduzca a mejorar la situación actual de la educación, condicionada de manera desfavorable por los factores contextuales del entorno interno y externo que la han relegado a un estancamiento en su desarrollo.This work intends to propose some Geostrategic Outlines for the Educational Development of the Border Area: Páez District of the Zulia State, which derive from the analysis of the factors that condition the educational development in the District, where the educational, economic, sociocultural, demographic and natural-physical aspects of that border reality are identified through the descriptive-exploratory methodology. Two stratified samples were worked, one of the educational personnel of the educational institutions located in the area and the other one, which includes the parents and representatives of the students attending those institutions. The techniques and instruments employed were the direct observation of intervening elements and the survey type questionnaire, which allowed to obtain the necessary inputs to determine why that border area requires a geostrategical proposal, with prospective vision that, supported in the systemic and holistic focuses, drives to improve the current situation of the education, conditioned in an unfavorable way for the contextual factors of the internal and external environment that have relegated it to a stagnation in its development.
- Published
- 2001
30. Las cátedras libres en el contexto de la reforma universitarialatinoamericana
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Rincón, Ismelda, Atencio, Maxula, Ortega, Sheila, Rincón, Ismelda, Atencio, Maxula, and Ortega, Sheila
- Abstract
Este artículo  analiza  las Cátedras  Libres en el contexto  de la Reforma Universitaria  Latinoamericana,  considerando ese término como  una  alternativa  de ejercer libre cátedra.  El tipo de investigación  es descriptiva-documental.  Las unidades de análisis fueron las fuentes secundarias  en físico y vía web.  Del análisis crítico, resultó:  a) existe vinculación  entre la Institución  Libre de Enseñanza  de España y los reformistas  de la Universidad  de Córdoba; b) En 1918 se asume la especificidad  de las Cátedras  Libres en el Manifiesto Laminar  de Córdoba  dando  inicio a la reforma  universitaria  latinoamericana, en la cual se fueron  incluyeron  progresivamente  las Cátedras  Libres.
- Published
- 2015
31. Valoración ética institucional del discurso del Dr. Jesús Enrique Lossada en la reapertura de la Universidad del Zulia
- Author
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Rincón, Imelda, Ortega, Sheila, Rincón, Imelda, and Ortega, Sheila
- Abstract
En el presente trabajo se planteó como objetivo general, analizar la valoración ética institucional en el discurso del Dr. Jesús Enrique Lossada con motivo de la reapertura de la Universidad del Zulia en el año 1946, basado en la metodología descriptiva y documental, con la técnica de análisis de contenido de sus escritos sobre la historia institucional de esa universidad. Se detectaron cuatro valores éticos: a) sostenibilidad por su posicionamiento y permanencia como máxima casa de estudio de la región occidental de Venezuela a través de sus aportes científico, culturales y cívicos; b) autonomía por la libertad académica; c) participación por su carácter público; d) pertinencia por su compromiso con el estado Zulia; e) libertad por su apertura a la pluralidad de las ideas. Al analizar el discurso del Rector Lossada se consideró la necesidad de rescatar los valores éticos institucionales expuestos en su discurso para fortalecer el compromiso académico de la Universidad del Zulia con la patria venezolana.
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- 2015
32. RESPONSABILIDAD SOCIAL Y REFORMA UNIVERSITARIA EN EL PROCESO HISTÓRICO DE LA UNIVERSIDAD DEL ZULIA (1891-2000)
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Rincón, Imelda and Ortega, Sheila
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responsabilidad social, reforma universitaria, Universidad del Zulia - Abstract
La Responsabilidad Social Universitaria (RSU) puede entenderse como la capacidad que tiene launiversidad de difundir y aplicar un conjunto de principios y valores éticos que sustenten la formaciónestudiantil y el compromiso social del quehacer científico. Este trabajo tiene como objetivo identificar elreferido concepto en el lapso histórico comprendido entre 1891 y 2000. La metodología utilizada fue ladescriptiva-documental, delimitada en etapas tomando en consideración sus especificidades. Comoresultado se evidenció que en la Universidad del Zulia, el concepto de RSU asociada a sus ideas e idealesde reforma estuvo presente en los procesos históricos abordados.
- Published
- 2013
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33. Application of real-time PCR for the detection of Strongyloides spp. in clinical samples in a reference center in Spain
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Saugar, José M., primary, Merino, Francisco J., additional, Martín-Rabadán, Pablo, additional, Fernández-Soto, Pedro, additional, Ortega, Sheila, additional, Gárate, Teresa, additional, and Rodríguez, Esperanza, additional
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- 2015
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34. Pertinencia social de las cátedras libres en la Universidad del Zulia
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Rincón, Imelda, Aular de Durán, Judith, Ortega, Sheila, Rincón, Imelda, Aular de Durán, Judith, and Ortega, Sheila
- Abstract
En Venezuela, se requiere la vinculación de lo académico con el saber popular. La Universidad del Zulia ofrece esa oportunidad a través de las Cátedras Libres. Es por eso que este trabajo tiene como objetivo proponer estrategias integradoras de estos saberes. La metodología utilizada fue la descriptiva-documental. Los resultados fueron: a) se hace necesario optimizar las potencialidades de las Cátedras Libres, b) es pertinente caracterizar el entorno social de LUZ, c) se debe vincular cada una de las Cátedras Libres con el saber popular afín a su naturaleza académica, y d) las Cátedras Libres ameritan de una campaña de divulgación de sus actividades. Se concluye que se requiere la elaboración de estrategias metodológicas desarrolladas por parte de la Coordinación de las Cátedras Libres bajo las directrices de las políticas acadé- micas universitarias para lograr el desarrollo de su pertinencia social, por lo que se recomienda que cada una de las Cátedras Libres se articulen en esas políticas.
- Published
- 2014
35. Coverage of educational infrastructure in Paez municipality. Taking advantages in GIS
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Ortega, Sheila, Romero, Adelmo, and Marín, Hugo
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Revistas ,Educational infrastructure ,Ponencias [Revista Geoenseñanza] ,Núcleo Táchira (NUTULA) ,Geographic Information Systems (GIS) ,Spatial analysis ,Análisis espacial ,Sistema de Información Geográfica (SIG) ,Infraestructura educativa ,Revista Geoenseñanza - Abstract
CONTENIDO Presentación. Santiago Rivera, José Armando Conferencias La geografía y su enseñanza en el ámbito de los tiempos actuales. Cunill Grau, Pedro La geografía, su enseñanza y los cambios del mundo actual. Proposal for a methodology based on G.I.S. to determine the potential use of land. Trinca Fighera, Delfina Ponencias Reflexiones recientes de lo social y los desafíos en la enseñanza de la geografía. Medidate recent reflexions about social facts and the challenges in the teaching of geography. Peñalver B., Luis Fragmentos para un discurso pedagógico eco-cognitivo. Fragment for an echo-cognitive speech. Ugas Fermín, Gabriel Consideraciones preliminares para re-pensar una pedagogía crítica del saber social desde el perfil epistemológico de la perspectiva geohistórica. Preliminary considerations to re-think a critical pedagogy of the social knowledge from the epistemological prolile of the geohistoric perspective. Guzmán Moya, Elsa Algunos criterios para el análisis de textos escolares de geografía. Some approaches for the analysis of some geography school text books. Yovane de Simancas, Katia Fundamentación del diseño de materiales didácticos en geografía para el logro de aprendizajes significativos. Foundation of the design of didactic materials in geography for the achievement of significant learning. Ceballos García, Beatriz Investigación documental sobre los conceptos y su aplicación en la enseñanza de la geografía. Documental research on the concepts and its application in the teaching of geography. Grupo GEOPAIDEIA. Martínez P., Luz Mery; Pérez, Ana Griselda; R. de Moreno, Elsa Amanda; Torres de Cárdenas, Rosa; Rodríguez P., Liliana y Suárez, Luz Marina Globalidad - especificidad. Una visión para la escuela. Globalityspecificity. A vision for the school. Bartoli, Rosa A. Consideraciones generales sobre la organización del espacio. Planificación y ordenación territorial. General considerations about the space organization. Planning and territorial organization. Rangel S., Alida Geomática: un nuevo paradigma para el manejo de la información geográfica. Geomatic: a new paradigm for managing geographic information. Flores R., Ernesto J. El Corema: una herramienta para el estudio de la organización del espacio. The Choreme: A tool for the study of spatial organization. Martinez Q., Lucia W. Cobertura de la infraestructura educativa del Municipio Páez. Aprovechamiento de un Sistema de Información Geográfica. Coverage of educational infrastructure in Paez municipality. Taking advantages in GIS. Ortega, Sheila; Romero, Adelmo y Marín, Hugo El sistema de la F.A.O. para evaluación de tierras sustentado en Sistemas de Información Geográfica. The F.A.O. framework for land evaluation based on geographic information systems. Flores R., Ernesto J. Propuesta cartogeohistórica de la Parroquia Cecilio Acosta Municipio Maracaibo, (Estado Zulia) como producto de la ocupación del espacio. Cartogeohistoric proposal of the parroquia Cecilio Acosta -Maracaibo Municipality- Zulia as a product of the space occupation. Sayago, Aura A. La enseñanza de la geografía en los estudios de postgrado. The teaching of geography in the postgraduate studies. Santiago Rivera, José Armando Enseñanza de la geohistoria: una propuesta curricular de estudios de postgrado. The teaching of geohistory curricular proposal for postgraduate studies. Loreto, Carlos El desarrollo curricular del Postgrado en Geografía en la Universidad del Zulia y su relación con las actividades de Pregrado. Apitz de Parra, Alicia 177-186 semestral Nivel analítico
- Published
- 2006
36. Responsabilidad social y reforma universitaria en el proceso histórico de la Universidad del Zulia 1891-2000: patrimonio para la transformación académica.
- Author
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Rincón, Imelda, Ortega, Sheila, Rincón, Imelda, and Ortega, Sheila
- Abstract
La Responsabilidad Social Universitaria -RSU- puede entenderse como la capacidad que tiene la universidad de difundir y aplicar un conjunto de principios y valores éticos que sustentan la formación estudiantil y el compromiso social del quehacer científico. Este trabajo tiene como objetivo identificar el referido concepto en el lapso histórico comprendido entre 1891 y 2000. La metodología utilizada fue la descriptiva-documental, delimitada en etapas, tomando en consideración sus especificidades. Como resultado, se evidenció que en la Universidad del Zulia, el concepto de RSU asociado a sus ideas e ideales de reforma estuvo presente en los procesos históricos abordados, lo cual constituye un valor patrimonial.
- Published
- 2013
37. Responsabilidad social y reforma universitaria en el proceso histórico de la Universidad del Zulia (1891-2000)
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Rincón Finol, Imelda, Ortega, Sheila, Rincón Finol, Imelda, and Ortega, Sheila
- Published
- 2013
38. Discurso político del maestro Jesús Enrique Lossada
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Rincón, Imelda, Ortega, Sheila, Rincón, Imelda, and Ortega, Sheila
- Abstract
En el presente trabajo se planteó como objetivo general, analizar el discurso político del maestro Jesús Enrique Lossada a partir de su obra literaria, sus escritos políticos y filosóficos. Se detectó lo siguiente en el discurso político del maestro Jesús Enrique Lossada: a) su visión social está sustentada en tres ideas fundamentales: libertad, igualdad y fraternidad, b) su pertinencia en el contexto venezolano de la primera mitad del siglo XX, puede afirmarse que respondió a las necesidades socio-contextuales de la época; c) en cuanto a su vigencia en el presente se puede considerar que se adelantó a los procesos políticos actuales. Al analizar el discurso político del maestro Lossada se consideró la necesidad de rescatar los valores intelectuales y éticos de la actividad política vinculados a los procesos de la educación y el trabajo.
- Published
- 2012
39. SOCIAL RESPONSIBILITY AND UNIVERSITY REFORM IN THE ZULIA UNIVERSITY HISTORIC PROCESS (1891-2000).
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Rincón, Imelda and Ortega, Sheila
- Subjects
- *
UNIVERSITIES & colleges , *SOCIAL responsibility , *SCIENTIFIC method , *DOCUMENTARY credit - Abstract
University Social Responsibility (USR) can be understood as the ability of the university to disseminate and implement a set of ethical principles and values that support student training and social engagement of scientific endeavor. This work aims to identify that concept in the historical period between 1891 and 2000. The methodology used was descriptive documentary, bounded on stage taking into account their specificities. Results evidenced that at the University of Zulia, the concept of RSU associated with its ideas and ideals of reform was present in the historical processes addressed. [ABSTRACT FROM AUTHOR]
- Published
- 2013
40. Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect Leishmania spp.
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Chicharro C, Nieto J, Miguelañez S, Garcia E, Ortega S, Peña A, Rubio JM, and Flores-Chavez M
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- Animals, Humans, Real-Time Polymerase Chain Reaction methods, Spain, Reproducibility of Results, DNA, Protozoan genetics, DNA, Protozoan analysis, Sensitivity and Specificity, Mammals, Leishmania genetics, Leishmaniasis diagnosis
- Abstract
Leishmaniasis is an endemic parasitic disease in at least 98 countries. In Spain, it is considered a zoonosis caused by Leishmania infantum, with an annual incidence of 0.62 cases/100,000 inhabitants. The predominant clinical manifestations are the cutaneous (CL) and visceral forms (VL), and the diagnosis is performed by parasitological, serological, and molecular tests. At the WHO Collaborating Center for Leishmaniasis (WHOCCLeish), routine diagnostic tests are based on a nested PCR (Ln-PCR), culture, and serological tests. To simplify our PCR protocol, we aimed to develop and validate a ready-to-use nested gel-form PCR (LeishGelPCR) and a duplex real-time PCR (qPCR) that allowed simultaneous detection of Leishmania and mammalian DNA as an internal control (Leish-qPCR). Clinical validation was performed in 200 samples from the WHOCCLeish collection; 92 and 85 out of 94 and 87 samples were positive by LeishGelPCR and Leish-qPCR, respectively, showing a sensitivity of 98% in both approaches. The specificity was 100% for LeishGelPCR and 98% for Leish-qPCR. The limits of detection of both protocols were similar (0.5 and 0.2 parasites/reaction). Parasite loads in VL and CL forms were similar, although high loads were observed when invasive samples were tested. In conclusion, LeishGelPCR and Leish-qPCR showed excellent performance in the diagnosis of leishmaniasis. These new forms of 18S rRNA gene PCR are equivalent to Ln-PCR and can be introduced in the algorithm for CL and VL diagnosis. IMPORTANCE Although the gold standard for diagnosis of leishmaniasis is the microscopic observation of amastigotes, molecular techniques are becoming a cost-efficient alternative. Currently, PCR is a routine resource that is used in many reference microbiology laboratories. In this article, we have described two ways to improve the reproducibility and usability of the molecular detection of Leishmania spp. These new approaches could be introduced even in middle- and low-resource laboratories; one is a ready-to-use gel-form system of a nested PCR and the other is a real-time PCR. We show why molecular diagnosis is the best methodology to confirm a clinical suspicion of leishmaniasis with higher sensitivity than traditional methods, thus facilitating early diagnosis and timely treatment of human leishmaniasis., Competing Interests: The authors declare no conflict of interest.
- Published
- 2023
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41. Zoonotic Enterocytozoon bieneusi genotypes in free-ranging and farmed wild ungulates in Spain.
- Author
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Dashti A, Santín M, Köster PC, Bailo B, Ortega S, Imaña E, Habela MÁ, Rivero-Juarez A, Vicente J, Arnal MC, de Luco DF, Morrondo P, Armenteros JA, Balseiro A, Cardona GA, Martínez-Carrasco C, Ortiz JA, Calero-Bernal R, Carmena D, and González-Barrio D
- Subjects
- Animals, Animals, Wild, China epidemiology, Feces, Genotype, Humans, Phylogeny, Prevalence, Sheep, Spain epidemiology, Sus scrofa, Swine, Deer parasitology, Enterocytozoon genetics, Microsporidiosis epidemiology, Microsporidiosis veterinary, Sheep Diseases, Swine Diseases epidemiology
- Abstract
Microsporidia comprises a diverse group of obligate, intracellular, and spore-forming parasites that infect a wide range of animals. Among them, Enterocytozoon bieneusi is the most frequently reported species in humans and other mammals and birds. Data on the epidemiology of E. bieneusi in wildlife are limited. Hence, E. bieneusi was investigated in eight wild ungulate species present in Spain (genera Ammotragus, Capra, Capreolus, Cervus, Dama, Ovis, Rupicapra, and Sus) by molecular methods. Faecal samples were collected from free-ranging (n = 1058) and farmed (n = 324) wild ungulates from five Spanish bioregions. The parasite was detected only in red deer (10.4%, 68/653) and wild boar (0.8%, 3/359). Enterocytozoon bieneusi infections were more common in farmed (19.4%, 63/324) than in wild (1.5%, 5/329) red deer. A total of 11 genotypes were identified in red deer, eight known (BEB6, BEB17, EbCar2, HLJD-V, MWC_d1, S5, Type IV, and Wildboar3) and three novel (DeerSpEb1, DeerSpEb2, and DeerSpEb3) genotypes. Mixed genotype infections were detected in 15.9% of farmed red deer. Two genotypes were identified in wild boar, a known (Wildboar3) and a novel (WildboarSpEb1) genotypes. All genotypes identified belonged to E. bieneusi zoonotic Groups 1 and 2. This study provides the most comprehensive epidemiological study of E. bieneusi in Spanish ungulates to date, representing the first evidence of the parasite in wild red deer populations worldwide. Spanish wild boars and red deer are reservoir of zoonotic genotypes of E. bieneusi and might play an underestimated role in the transmission of this microsporidian species to humans and other animals., (© The Author(s) 2022. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.)
- Published
- 2022
- Full Text
- View/download PDF
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