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17 results on '"Otaizo-Carrasquero, Francisco"'

1. Human CCR6 +Th cells show both an extended stable gradient of Th17 activity and imprinted plasticity

Author

Singh, Satya, primary, Parween, Farhat, additional, Edara, Nithin, additional, Zhang, Hongwei H, additional, Chen, Jinguo, additional, Otaizo-Carrasquero, Francisco, additional, Cheng, Debby, additional, Oppenheim, Nicole A, additional, Ransier, Amy, additional, Zhu, Wenjun, additional, Shamsaddini, Amirhossein, additional, Gardina, Paul J, additional, Darko, Samuel W, additional, Singh, Tej Pratap, additional, Douek, Daniel C, additional, Myers, Timothy G, additional, and Farber, Joshua M, additional

Published
2023
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3. Human CCR6+ Th Cells Show Both an Extended Stable Gradient of Th17 Activity and Imprinted Plasticity

Author

Singh, Satya P., primary, Parween, Farhat, additional, Edara, Nithin, additional, Zhang, Hongwei H., additional, Chen, Jinguo, additional, Otaizo-Carrasquero, Francisco, additional, Cheng, Debby, additional, Oppenheim, Nicole A., additional, Ransier, Amy, additional, Zhu, Wenjun, additional, Shamsaddini, Amirhossein, additional, Gardina, Paul J., additional, Darko, Samuel W., additional, Singh, Tej Pratap, additional, Douek, Daniel C., additional, Myers, Timothy G., additional, and Farber, Joshua M., additional

Published
2023
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4. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

Author

Parween, Farhat, primary, Singh, Satya P., additional, Zhang, Hongwei H, additional, Kathuria, Nausheen, additional, Otaizo-Carrasquero, Francisco A., additional, Shamsaddini, Amirhossein, additional, Gardina, Paul J., additional, Ganesan, Sundar, additional, Kabat, Juraj, additional, Lorenzi, Hernan A., additional, Myers, Timothy G., additional, and Farber, Joshua M., additional

Published
2023
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5. Human CCR6+Th cells show both an extended stable gradient of Th17 activity and imprinted plasticity

Author

Singh, Satya P., primary, Parween, Farhat, additional, Edara, Nithin, additional, Zhang, Hongwei H., additional, Chen, Jinguo, additional, Otaizo-Carrasquero, Francisco, additional, Cheng, Debby, additional, Oppenheim, Nicole A., additional, Ransier, Amy, additional, Zhu, Wenjun, additional, Shamsaddini, Amirhossein, additional, Gardina, Paul J., additional, Darko, Samuel W., additional, Singh, Tej Pratap, additional, Douek, Daniel C., additional, Myers, Timothy G., additional, and Farber, Joshua M., additional

Published
2023
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8. Regional Differences in Human Biliary Tissues and Corresponding In Vitro-Derived Organoids

Author

Rimland, Casey A, Tilson, Samantha G, Morell, Carola M, Tomaz, Rute A, Lu, Wei-Yu, Adams, Simone E, Georgakopoulos, Nikitas, Otaizo-Carrasquero, Francisco, Myers, Timothy G, Ferdinand, John R, Gieseck, Richard L, Sampaziotis, Fotios, Tysoe, Olivia C, Ross, Alexander, Kraiczy, Judith M, Wesley, Brandon, Muraro, Daniele, Zilbauer, Matthias, Oniscu, Gabriel C, Hannan, Nicholas RF, Forbes, Stuart J, Saeb-Parsy, Kourosh, Wynn, Thomas A, Vallier, Ludovic, Rimland, Casey A [0000-0002-5187-6921], Myers, Timothy G [0000-0002-7059-5906], Gieseck, Richard L [0000-0003-0200-581X], Tysoe, Olivia C [0000-0002-1061-1484], Forbes, Stuart J [0000-0003-3715-2561], and Apollo - University of Cambridge Repository

Subjects
Common Bile Duct, Keratin-19, Tissue and Organ Procurement, Gallbladder, Cell Differentiation, Epithelial Cells, Organoids, Mice, Bile Ducts, Intrahepatic, Gene Expression Regulation, Liver, Bile Ducts, Extrahepatic, Animals, Bile, Humans, RNA-Seq
Abstract

BACKGROUND AND AIMS: Organoids provide a powerful system to study epithelia in vitro. Recently, this approach was applied successfully to the biliary tree, a series of ductular tissues responsible for the drainage of bile and pancreatic secretions. More precisely, organoids have been derived from ductal tissue located outside (extrahepatic bile ducts; EHBDs) or inside the liver (intrahepatic bile ducts; IHBDs). These organoids share many characteristics, including expression of cholangiocyte markers such as keratin (KRT) 19. However, the relationship between these organoids and their tissues of origin, and to each other, is largely unknown. APPROACH AND RESULTS: Organoids were derived from human gallbladder, common bile duct, pancreatic duct, and IHBDs using culture conditions promoting WNT signaling. The resulting IHBD and EHBD organoids expressed stem/progenitor markers leucine-rich repeat-containing G-protein-coupled receptor 5/prominin 1 and ductal markers KRT19/KRT7. However, RNA sequencing revealed that organoids conserve only a limited number of regional-specific markers corresponding to their location of origin. Of particular interest, down-regulation of biliary markers and up-regulation of cell-cycle genes were observed in organoids. IHBD and EHBD organoids diverged in their response to WNT signaling, and only IHBDs were able to express a low level of hepatocyte markers under differentiation conditions. CONCLUSIONS: Taken together, our results demonstrate that differences exist not only between extrahepatic biliary organoids and their tissue of origin, but also between IHBD and EHBD organoids. This information may help to understand the tissue specificity of cholangiopathies and also to identify targets for therapeutic development.

Published
2021

9. Regional Differences in Human Biliary Tissues and Corresponding In Vitro–Derived Organoids

Author

Rimland, Casey A., primary, Tilson, Samantha G., additional, Morell, Carola M., additional, Tomaz, Rute A., additional, Lu, Wei‐Yu, additional, Adams, Simone E., additional, Georgakopoulos, Nikitas, additional, Otaizo‐Carrasquero, Francisco, additional, Myers, Timothy G., additional, Ferdinand, John R., additional, Gieseck, Richard L., additional, Sampaziotis, Fotios, additional, Tysoe, Olivia C., additional, Ross, Alexander, additional, Kraiczy, Judith M., additional, Wesley, Brandon, additional, Muraro, Daniele, additional, Zilbauer, Matthias, additional, Oniscu, Gabriel C., additional, Hannan, Nicholas R.F., additional, Forbes, Stuart J., additional, Saeb‐Parsy, Kourosh, additional, Wynn, Thomas A., additional, and Vallier, Ludovic, additional

Published
2021
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10. Therapeutic responses to Roseomonas mucosa in atopic dermatitis may involve lipid-mediated TNF-related epithelial repair

Author

Myles, Ian A., primary, Castillo, Carlo R., additional, Barbian, Kent D., additional, Kanakabandi, Kishore, additional, Virtaneva, Kimmo, additional, Fitzmeyer, Emily, additional, Paneru, Monica, additional, Otaizo-Carrasquero, Francisco, additional, Myers, Timothy G., additional, Markowitz, Tovah E., additional, Moore, Ian N., additional, Liu, Xue, additional, Ferrer, Marc, additional, Sakamachi, Yosuke, additional, Garantziotis, Stavros, additional, Swamydas, Muthulekha, additional, Lionakis, Michail S., additional, Anderson, Erik D., additional, Earland, Noah J., additional, Ganesan, Sundar, additional, Sun, Ashleigh A., additional, Bergerson, Jenna R.E., additional, Silverman, Robert A., additional, Petersen, Maureen, additional, Martens, Craig A., additional, and Datta, Sandip K., additional

Published
2020
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11. PAX1 is essential for development and function of the human thymus

Author

Yamazaki, Yasuhiro, primary, Urrutia, Raul, additional, Franco, Luis M., additional, Giliani, Silvia, additional, Zhang, Kejian, additional, Alazami, Anas M., additional, Dobbs, A. Kerry, additional, Masneri, Stefania, additional, Joshi, Avni, additional, Otaizo-Carrasquero, Francisco, additional, Myers, Timothy G., additional, Ganesan, Sundar, additional, Bondioni, Maria Pia, additional, Ho, Mai Lan, additional, Marks, Catherine, additional, Alajlan, Huda, additional, Mohammed, Reem W., additional, Zou, Fanggeng, additional, Valencia, C. Alexander, additional, Filipovich, Alexandra H., additional, Facchetti, Fabio, additional, Boisson, Bertrand, additional, Azzari, Chiara, additional, Al-Saud, Bander K., additional, Al-Mousa, Hamoud, additional, Casanova, Jean Laurent, additional, Abraham, Roshini S., additional, and Notarangelo, Luigi D., additional

Published
2020
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15. Therapeutic responses to Roseomonas mucosain atopic dermatitis may involve lipid-mediated TNF-related epithelial repair

Author

Myles, Ian A., Castillo, Carlo R., Barbian, Kent D., Kanakabandi, Kishore, Virtaneva, Kimmo, Fitzmeyer, Emily, Paneru, Monica, Otaizo-Carrasquero, Francisco, Myers, Timothy G., Markowitz, Tovah E., Moore, Ian N., Liu, Xue, Ferrer, Marc, Sakamachi, Yosuke, Garantziotis, Stavros, Swamydas, Muthulekha, Lionakis, Michail S., Anderson, Erik D., Earland, Noah J., Ganesan, Sundar, Sun, Ashleigh A., Bergerson, Jenna R.E., Silverman, Robert A., Petersen, Maureen, Martens, Craig A., and Datta, Sandip K.

Abstract

Roseomonas mucosatreatment in children with atopic dermatitis is associated with clinical improvements and potentially lipid-mediated TNF signaling.

Published
2020
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16. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells.

Author

Parween F, Singh SP, Zhang HH, Kathuria N, Otaizo-Carrasquero FA, Shamsaddini A, Gardina PJ, Ganesan S, Kabat J, Lorenzi HA, Myers TG, and Farber JM

Abstract

Pro-inflammatory T cells co-express multiple chemokine receptors, but the distinct functions of individual receptors on these cells are largely unknown. Human Th17 cells uniformly express the chemokine receptor CCR6, and we discovered that the subgroup of CD4 + CCR6 + cells that co-express CCR2 possess a pathogenic Th17 signature, can produce inflammatory cytokines independent of TCR activation, and are unusually efficient at transendothelial migration (TEM). The ligand for CCR6, CCL20, was capable of binding to activated endothelial cells (ECs) and inducing firm arrest of CCR6 + CCR2 + cells under conditions of flow - but CCR6 could not mediate TEM. By contrast, CCL2 and other ligands for CCR2, despite being secreted from both luminal and basal sides of ECs, failed to bind to the EC surfaces - and CCR2 could not mediate arrest. Nonetheless, CCR2 was required for TEM. To understand if CCR2's inability to mediate arrest was due solely to an absence of EC-bound ligands, we generated a CCL2-CXCL9 chimeric chemokine that could bind to the EC surface. Although display of CCL2 on the ECs did indeed lead to CCR2-mediated arrest of CCR6 + CCR2 + cells, activating CCR2 with surface-bound CCL2 blocked TEM. We conclude that mediating arrest and TEM are mutually exclusive activities of chemokine receptors and/or their ligands that depend, respectively, on chemokines that bind to the EC luminal surfaces versus non-binding chemokines that form transendothelial gradients under conditions of flow. Our findings provide fundamental insights into mechanisms of lymphocyte extravasation and may lead to novel strategies to block or enhance their migration into tissue., Competing Interests: The authors declare no competing financial interests.

Published
2023
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17. Human CCR6 + Th cells show both an extended stable gradient of Th17 activity and imprinted plasticity.

Author

Singh SP, Parween F, Edara N, Zhang HH, Chen J, Otaizo-Carrasquero F, Cheng D, Oppenheim NA, Ransier A, Zhu W, Shamsaddini A, Gardina PJ, Darko SW, Singh TP, Douek DC, Myers TG, and Farber JM

Abstract

Th17 cells have been investigated in mice primarily for their contributions to autoimmune diseases. However, the pathways of differentiation of Th17 and related (type 17) cells and the structure of the type 17 memory population in humans are not well understood; such understanding is critical for manipulating these cells in vivo . By exploiting differences in levels of surface CCR6, we found that human type 17 memory cells, including individual T cell clonotypes, form an elongated continuum of type 17 character along which cells can be driven by increasing RORγt. This continuum includes cells preserved within the memory pool with potentials that reflect the early preferential activation of multiple over single lineages. The CCR6 + cells' phenotypes and epigenomes are stable across cell divisions under homeostatic conditions. Nonetheless, activation in polarizing and non-polarizing conditions can yield additional functionalities, revealing, respectively, both environmentally induced and imprinted mechanisms that contribute differentially across the continuum to yield the unusual plasticity ascribed to type 17 cells.

Published
2023
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