515 results on '"Otoi, Takeshige"'
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2. Effects of centrifugation treatment before electroporation on gene editing in pig embryos
3. Evaluation of culture methods and chemical reagent combinations on CRISPR/Cas9 gene editing systems by lipofection in pig zygotes
4. Programmed cell death-1-modified pig developed using electroporation-mediated gene editing for in vitro fertilized zygotes
5. Avoiding aggregation of human bone marrow–derived mesenchymal stem cells stored in cell preservation solutions
6. GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system
7. Selection of spermatozoa with high motility and quality from bovine frozen-thawed semen using the centrifuge-free device
8. Evaluating variations in bilirubin glucuronidation activity by protease inhibitors in canine and human primary hepatocytes cultured in a 3D culture system
9. Relationship between oxygen partial pressure and inhibition of cell aggregation of human adipose tissue-derived mesenchymal stem cells stored in cell preservation solutions
10. GEEP Method: An Optimized Electroporation-Mediated Gene Editing Approach for Establishment of Knockout Pig Lines
11. Dimethyl sulfoxide-free cryopreservation solution containing trehalose, dextran 40, and propylene glycol for therapy with human adipose tissue-derived mesenchymal stromal cells
12. Comparative analysis of bilirubin glucuronidation activity in canine and human primary hepatocytes using a 3D culture system
13. Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation
14. Porcine embryo development and inactivation of microorganisms after ultraviolet-C irradiation at 228 nm
15. Designing a diagnostic method to predict the optimal artificial insemination timing in cows using artificial intelligence
16. Reproductive cycle and in vitro maturation of canine oocyte: A meta-analysis approach
17. Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system
18. Effects of green tea polyphenols and α-tocopherol on the quality of chilled cat spermatozoa and sperm IZUMO1 protein expression during long-term preservation
19. Heat Shock Related Protein Expression in Abdominal Testes of Asian Elephant (Elephas maximus).
20. Quality and fertilizing ability of frozen–thawed porcine sperm separated using a migration sedimentation method.
21. One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs
22. Effects of ergothioneine supplementation on the quality of liquid-preserved and frozen-thawed boar semen
23. Development of porcine embryos cultured in media irradiated with ultraviolet‐C
24. Impact of redox status of donor cows before superovulation treatment on in vivo embryo production
25. Comparative analysis of bilirubin glucuronidation activity in 2D- and 3D-cultured human hepatocellular carcinoma HepG2 cells
26. Genome mutation after the introduction of the gene editing by electroporation of Cas9 protein (GEEP) system into bovine putative zygotes
27. Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
28. Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events
29. Generation of mutant pigs by lipofection-mediated genome editing in embryos
30. A change in the steroid metabolic pathway in human testes showing deteriorated spermatogenesis
31. Selection of spermatozoa with high motility and quality from bovine frozen-thawed semen using the centrifuge-free device
32. Development and characterization of islet‐derived mesenchymal stem cells from clinical grade neonatal porcine cryopreserved islets
33. Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes
34. Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation
35. Development and characterization of islet‐derived mesenchymal stem cells from clinical grade neonatal porcine cryopreserved islets.
36. Development of porcine embryos cultured in media irradiated with ultraviolet‐C.
37. Mosaic TP53 Mutation on Tumour Development in Pigs: A Case Study
38. Effects of curcumin supplementation on quality of porcine spermatozoa irradiated with ultraviolet‐C at 228 nm during liquid preservation
39. The Effects of an In Vitro Oocyte Maturation System and Chlorogenic Acid Supplementation during Embryo Culture on the Development of Porcine Cloned Embryos Derived from Native Vietnamese Ban Pigs
40. Development and Genome Mutation of Bovine Zygotes Vitrified Before and After Genome Editing via Electroporation
41. Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes
42. Effects of ergothioneine supplementation on the quality of liquid-preserved and frozen-thawed boar semen.
43. Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN
44. Comparison of chemically mediated CRISPR/Cas9 gene editing systems using different nonviral vectors in porcine embryos
45. Efficiency of genetic modification in gene‐knockout sperm‐derived zygotes followed by electroporation of guide RNA targeting the same gene
46. Astaxanthin present in the maturation medium reduces negative effects of heat shock on the developmental competence of porcine oocytes
47. Relationship between GnRH-induced LH increase profiles in the serum and vaginal mucus of Japanese Black beef cows
48. Efficacy of Abdominal Ultrasonography for Differentiation of Gastrointestinal Diseases in Calves
49. Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods
50. Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs
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