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Additional file 2: Figure S1. Distribution of bacterial taxa at the phylum level and genus level; Figure S2. Reveal of characteristic bacterial taxa based on LDA Effect Size (LEfSe) analysis between Healthy-LB and PCOS-LB, between Healthy-HB and PCOS-HB; Figure S3. Distribution of fungal taxa at the phylum level and genus level; Figure S4. Reveal of characteristic fungal taxa based on LDA Effect Size (LEfSe) analysis between Healthy-LB and PCOS-LB; Figure S5. Reveal of characteristic fungal taxa based on LDA Effect Size (LEfSe) analysis between Healthy-HB and PCOS-HB; Figure S6. Serum metabolome changes in disease and healthy subjects; Figure S7. Reveal of characteristic metabolites based on LDA Effect Size (LEfSe) analysis between Healthy-LB and PCOS-LB; Figure S8. Reveal of characteristic metabolites based on LDA Effect Size (LEfSe) analysis between Healthy-HB and PCOS-HB; Figure S9. The bacterial genera and predicted pathway features were excavated on the base of Wilcox test comparison and Mean Decrease Gini by random forest importance parameter; Figure S10. The fungal genera and metabolites were excavated on the base of Wilcox test comparison and Mean Decrease Gini by random forest importance parameter; Figure S11. Correlation between bacterial diversity and fungal diversity.