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4 results on '"Ouwerkerk-van Velzen, MCM"'

2. Fetal cell detection in maternal blood: A study in 236 samples using erythroblast morphology, DAB and HbF staining, and FISH analysis

Author

Oosterwijk, JC, Mesker, WE, Ouwerkerk-van Velzen, MCM, Knepfle, CFHM, Wiesmeijer, KC, Beverstock, GC, van Ommen, GJB, Kanhai, HHH, Tanke, HJ, Damage and Repair in Cancer Development and Cancer Treatment (DARE), and Targeted Gynaecologic Oncology (TARGON)

Subjects
fetal hemoglobin, chorionic villus sampling, prenatal diagnosis, hemoglobin gamma-chain, FLOW-CYTOMETRY, POLYMERASE CHAIN-REACTION, IN-SITU HYBRIDIZATION, PERIPHERAL-BLOOD, DIAGNOSIS, 3,3-diaminobenzidin, TROPHOBLAST, immunocytochemistry, FISH, rare event detection, PRENATAL SEX DETERMINATION, nucleated red blood cell, NUCLEATED ERYTHROCYTES, fetal-maternal transfusion, ENRICHMENT, PREGNANT-WOMEN
Abstract

A protocol to detect fetal nucleated red blood cells (NRBCs) was tested in 217 pregnant women and in 19 nonpregnant controls. All the pregnant women were sampled after chorionic villus sampling (CVS); 20 were also sampled pre-CVS. NRBC recognition was based upon morphology by using staining of hemoglobin with 3,3-diaminobenzidin (DAB) or by immunocytochemical staining for fetal hemoglobin (HbF), This was combined with FISH analysis for both the X- and Y-chromosomes on the same cells. Progressive refinement of the methods increased the number of cases where NRBCs were detected from 53% (DAB) to 75% and 78% for DAB and HbF staining, respectively, with on average 43 NRBCs (range, 1-220), DAB gave a slightly higher yield than HbF in the lower cell count range (

Published
1998

3. Detectie van foetale erythroblasten in moederlijk bloed

Author

Oosterwijk, J.C., MESKER, W.E., Ouwerkerk-van Velzen, MCM, Knepfle, CFHM, Wiesmeijer, C.C., Beverstock, G.C., van Ommen, G.J.B., Kanhai, H.H.H., Tanke, HJ, Damage and Repair in Cancer Development and Cancer Treatment (DARE), and Targeted Gynaecologic Oncology (TARGON)

Published
1998

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