Search

A microfluorimetric system was used to study rat bone marrow erythroblastic islands (EI) and macrophages. Measurement of fluorescence from acridine-orange-treated cells was performed on the microscope LUMAM-E3 (LOMO, Leningrad) at 530-550 and 630-650 nm. The intensity of fluorescence in 530-550 nm range depended on the intensity of proliferative processes in EI, that at 630-650 nm was associated with activity of lysosomal apparatus of EI macrophages. Erythroblast amplification in EI is parallel both to enhancement of fluorescence intensity at 530-550 nm and 630-650 nm. Intensity of fluorescence of different bone marrow macrophages was evaluated. It is suggested that a part of bone marrow macrophages has high affinity to erythroid tissue.

Eight physically trained male volunteers living in tropical countries were examined. Being under high physical load they were given the diet (for 3 weeks) containing food concentrates and canned food with a caloric value of 3860 calories. Energy expenditures amounted to 3600-4100 kcal/day. According to the physiological data the central and peripheral nervous systems and higher nervous activity remained unchanged throughout the entire observation period. As shown by the PWC170 test, the orthostatic stability and exercise tolerance were lowered at the initial period of the living in hot climate. Subjectively this was manifested by excessive fatigue toward the end of the working day. All the characteristics under study returned to normal by the 10th day of living in hot climate. The data obtained indicate that under the conditions of hot climate and appreciable physical loading, it is necessary to take into account the changes in body function in order to improve the diet for the people to be able to get adapted to the conditions in question.