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263 results on '"Overton W"'

2. High‐fidelity detection and sorting of nanoscale vesicles in viral disease and cancer

Author

Morales‐Kastresana, Aizea, Musich, Thomas A, Welsh, Joshua A, Telford, William, Demberg, Thorsten, Wood, James CS, Bigos, Marty, Ross, Carley D, Kachynski, Aliaksander, Dean, Alan, Felton, Edward J, Van Dyke, Jonathan, Tigges, John, Toxavidis, Vasilis, Parks, David R, Overton, W Roy, Kesarwala, Aparna H, Freeman, Gordon J, Rosner, Ariel, Perfetto, Stephen P, Pasquet, Lise, Terabe, Masaki, McKinnon, Katherine, Kapoor, Veena, Trepel, Jane B, Puri, Anu, Kobayashi, Hisataka, Yung, Bryant, Chen, Xiaoyuan, Guion, Peter, Choyke, Peter, Knox, Susan J, Ghiran, Ionita, Robert‐Guroff, Marjorie, Berzofsky, Jay A, and Jones, Jennifer C

Subjects
Biochemistry and Cell Biology, Biological Sciences, Nanotechnology, Bioengineering, Cancer, Nanofacs, sorting, flow cytometry, phenotyping, extracellular vesicles, Biochemistry and cell biology
Abstract

Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields of medicine as biomarkers and mediators of or treatments for disease. However, exosomes and small viruses fall below the detection limits of conventional flow cytometers due to the overlap of particle-associated scattered light signals with the detection of background instrument noise from diffusely scattered light. To identify, sort, and study distinct subsets of EVs and other nanoparticles, as individual particles, we developed nanoscale Fluorescence Analysis and Cytometric Sorting (nanoFACS) methods to maximise information and material that can be obtained with high speed, high resolution flow cytometers. This nanoFACS method requires analysis of the instrument background noise (herein defined as the "reference noise"). With these methods, we demonstrate detection of tumour cell-derived EVs with specific tumour antigens using both fluorescence and scattered light parameters. We further validated the performance of nanoFACS by sorting two distinct HIV strains to >95% purity and confirmed the viability (infectivity) and molecular specificity (specific cell tropism) of biological nanomaterials sorted with nanoFACS. This nanoFACS method provides a unique way to analyse and sort functional EV- and viral-subsets with preservation of vesicular structure, surface protein specificity and RNA cargo activity.

Published
2019

16. The forests of the United States : their use /

Author

Price, Overton W. (Overton Westfeldt), 1873-1914, Cox, William T. (William Thomas), 1878-1961, Kellogg, Royal S., United States. Forest Service, U.S. Department of Agriculture, National Agricultural Library, Price, Overton W. (Overton Westfeldt), 1873-1914, Cox, William T. (William Thomas), 1878-1961, Kellogg, Royal S., and United States. Forest Service

Subjects
Forest management, Forests and forestry, Internet resource, United States
Published
1909

17. The forests of the United States : their use

Author

Price, Overton W. (Overton Westfeldt), 1873-1914, Kellogg, R. S. (Royal Shaw), 1874-1965, Cox, William T. (William Thomas), 1878-1961, United States. Forest Service, U.S. Department of Agriculture, National Agricultural Library, Price, Overton W. (Overton Westfeldt), 1873-1914, Kellogg, R. S. (Royal Shaw), 1874-1965, Cox, William T. (William Thomas), 1878-1961, and United States. Forest Service

Subjects
Forest management, Forests and forestry, Internet resource, United States

19. First results for a novel superconducting imaging-surface sensor array

Author

Kraus, R.H., Jr., Flynn, E.R., Espy, M.A., Matlashov, A., Overton, W., Peters, M.V., and Ruminer, P.

Subjects
Superconducting quantum interference devices -- Testing, Superconductivity -- Research, Superconductors -- Magnetic properties, Business, Electronics, Electronics and electrical industries
Abstract

The fabrication of a superconducting imaging-surface system utilizing 12 coplanar thin-film superconducting quantum interference device (SQUID) magnetometers is presented. Sensor response measurements to 'point dipole' magnetic sources, including the uniform fields that were used to simulate ambient magnetic fields, performed according to the predicted values. The superconducting imaging-surface reduced the uniform ambient fields at the SQUID sensors by about a factor of ten.

Published
1999

27. Letters to the Editor

Author

Lindley, Dennis V., Overton, W. Scott, Stehman, Stephen V., Hedayat, A. S., Zumbo, Bruno D., Zimmerman, Donald W., McKean, Joseph W., Vidmar, Thomas J., Stone, Dan C., Ilderton, Robert B., Abramson, Lee R., Gottfried, Paul, and Konold, Clifford

Published
1996

41. First results for a novel superconducting imaging-surface sensor array

Author

Flynn, E. R., Kraus, R. H. Jr., Flynn E. R., Espy M. A., Matlashov A., Overton W., Peters M. V., Ruminer P., Matlashov, A., Espy, M. A., Overton, W., Peters, M. V., Ruminer, P., Kraus, R. R., Flynn E R., Espy M A., Peters M V., and Ruminer P

Abstract

A superconducting imaging-surface system was constructed using 12 coplanar thin-film SQUID magnetometers located parallel to and spaced 2 cm from a 25 cm diameter lead imaging-plane. Some measurements included two additional sensors on the ''back'' side of the superconducting imaging-plane to study the field symmetry for our system. Performance was measured in a shielded can and in the open laboratory environment. Data from this system has been used to: (a) understand the noise characteristics of the dewar-SQUID imaging plate arrangement, (b) to verify the imaging principle, (c) measure the background rejection factor of the imaging plane, and (d) compare superconducting materials for the imaging plane. A phantom source field was measured at the sensors as a function of phantom distance from the sensor array to verify the imaging theory. Both the shape and absolute value of the measured and predicted curves agree very well indicating the system is behaving as a gradiometer in accordance with theory. The output from SQUIDs located behind the imaging surface that sense background fields can be used for software or analog background cancellation. Fields arising from sources close to the imaging plane were shielded form the background sensors by more than a factor of 1000. Measurement of the symmetry of sensor sensitivity to uniform fields exactly followed theoretical predictions.

Published
1999

47. Sampling Methods for Multiresource Forest Inventory

Author

Overton, W. Scott

Subjects
Sampling Methods for Multiresource Forest Inventory (Book) -- Book reviews, Books -- Book reviews, Mathematics
Abstract

Hans T. Schreuder, Timothy G. Gregoire, and Geoffrey Wood. New York; John Wiley, 1993. xv + 446 pp. $79.95. Characterized in its Preface as 'a reference text for forest biometricians [...]

Published
1994

49. Statistical Methods in Soil and Land Resource Survey

Author

Overton, W. Scott

Subjects
Statistical Methods in Soil and Land Resource Survey (Nonfiction work) -- Book reviews, Books -- Book reviews, Mathematics
Abstract

R. Webster and M. A. Oliver. New York: Oxford University Press, 1990. 325 pp. $95 (cloth) $48 (paperback). This is a nice little book. It is written in a warm [...]

Published
1991

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