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Introduction: Sorghum bicolor: widely cultivated in Asia and Africa, faces increasing challenges from climate change, specifically from abiotic stresses like drought and salinity. This study evaluates how different sorghum genotypes respond to separate and combined stresses of drought and salinity. Methods: Carried out with three replications using a randomized complete block design, the experiment measured biochemical and physiological parameters, including stomatal conductance, chlorophyll content, and antioxidant enzyme activities. Molecular analysis focused on stress-responsive gene expression. Results: Results indicated enhanced stress responses under combined conditions, with significant variation in antioxidant enzymatic activities among genotypes. Genotype-specific osmotic adjustments were observed through proline and glycine betaine accumulation. Physiological parameters such as chlorophyll content, cell membrane stability, stomatal conductance, and water potential were critical indicators of stress tolerance. Gene expression analysis revealed upregulation of stress-responsive genes, particularly under combined stress conditions. Discussion: Correlation and principal component analysis analyses highlighted the interdependencies among traits, emphasizing their roles in oxidative stress mitigation. Samsorg-17 exhibited the highest resilience due to consistently high levels of catalase, superoxide dismutase, and glycine betaine, alongside superior physiological attributes. CRS-01 showed moderate resilience with the highest Na/K ratio and notable photosynthesis rate and relative water content, but was less consistent in biochemical markers under stress. Samsorg-42 demonstrated resilience under specific conditions but was generally less robust than Samsorg-17 across most indicators. These findings emphasize the importance of developing stress-resilient sorghum cultivars through targeted breeding programs to enhance tolerance to drought and salinity in sustainable agriculture. [ABSTRACT FROM AUTHOR]

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IntroductionSorghum bicolor: widely cultivated in Asia and Africa, faces increasing challenges from climate change, specifically from abiotic stresses like drought and salinity. This study evaluates how different sorghum genotypes respond to separate and combined stresses of drought and salinity.MethodsCarried out with three replications using a randomized complete block design, the experiment measured biochemical and physiological parameters, including stomatal conductance, chlorophyll content, and antioxidant enzyme activities. Molecular analysis focused on stress-responsive gene expression.ResultsResults indicated enhanced stress responses under combined conditions, with significant variation in antioxidant enzymatic activities among genotypes. Genotype-specific osmotic adjustments were observed through proline and glycine betaine accumulation. Physiological parameters such as chlorophyll content, cell membrane stability, stomatal conductance, and water potential were critical indicators of stress tolerance. Gene expression analysis revealed upregulation of stress-responsive genes, particularly under combined stress conditions.DiscussionCorrelation and principal component analysis analyses highlighted the interdependencies among traits, emphasizing their roles in oxidative stress mitigation. Samsorg-17 exhibited the highest resilience due to consistently high levels of catalase, superoxide dismutase, and glycine betaine, alongside superior physiological attributes. CRS-01 showed moderate resilience with the highest Na/K ratio and notable photosynthesis rate and relative water content, but was less consistent in biochemical markers under stress. Samsorg-42 demonstrated resilience under specific conditions but was generally less robust than Samsorg-17 across most indicators. These findings emphasize the importance of developing stress-resilient sorghum cultivars through targeted breeding programs to enhance tolerance to drought and salinity in sustainable agriculture.

Zinc oxide nanoparticles (ZnO NPs) exhibit diverse applications, including antimicrobial, UV-blocking, and catalytic properties, due to their unique structure and properties. This study focused on the characterization of zinc oxide nanoparticles (ZnO NPs) synthesized from Juglans regia leaves and their application in mitigating the impact of simultaneous infection by Meloidogyne arenaria (root-knot nematode) and Macrophomina phaseolina (root-rot fungus) in cowpea plants. The characterization of ZnO NPs was carried out through various analytical techniques, including UV–visible spectrophotometry, Powder-XRD analysis, FT-IR spectroscopy, and SEM-EDX analysis. The study confirmed the successful synthesis of ZnO NPs with a hexagonal wurtzite structure and exceptional purity. Under in vitro conditions, ZnO NPs exhibited significant nematicidal and antifungal activities. The mortality of M. arenaria juveniles increased with rising ZnO NP concentrations, and a similar trend was observed in the inhibition of M. phaseolina mycelial growth. SEM studies revealed physical damage to nematodes and structural distortions in fungal hyphae due to ZnO NP treatment. In infected cowpea plants, ZnO NPs significantly improved plant growth parameters, including plant length, fresh mass, and dry mass, especially at higher concentrations. Leghemoglobin content and the number of root nodules also increased after ZnO NP treatment. Additionally, ZnO NPs reduced gall formation and egg mass production by M. arenaria nematodes and effectively inhibited the growth of M. phaseolina in the roots. Furthermore, histochemical analyses demonstrated a reduction in oxidative stress, as indicated by decreased levels of reactive oxygen species (ROS) and lipid peroxidation in ZnO NP-treated plants. These findings highlight the potential of green-synthesized ZnO NPs as an eco-friendly and effective solution to manage disease complex in cowpea caused by simultaneous nematode and fungal infections. [ABSTRACT FROM AUTHOR]

Seed priming with a composite of iron oxide (Fe3O4) and silicon dioxide (SiO2) nanoparticles (NPs) is an innovative technique to mitigate cadmium (Cd) and chromium (Cr) uptake in plants from rooting media. The current study explored the impact of seed priming with varying levels of Fe3O4 NPs, SiO2 NPs, and Fe3O4-SiO2 nanocomposites on Cd and Cr absorption and phytotoxicity, metal-induced oxidative stress mitigation, growth and biomass yield of spinach (Spinacia oleracea L.). The results showed that seed priming with the optimum level of 100 mg L−1 of Fe3O4-SiO2 nanocomposites significantly (p ≤ 0.05) increased root dry weight (144 %), shoot dry weight (243 %) and leaf area (34.4 %) compared to the control, primarily by safeguarding plant's photosynthetic machinery, oxidative stress and phytotoxicity of metals. Plants treated with this highest level of Fe3O4-SiO2 nanocomposites exhibited a substantial increase in photosynthetic and gas exchange indices of spinach plants and enhanced activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) antioxidant enzymes by 45 %, 48 %, and 60 %, respectively. Correspondingly, the relative gene expression levels of SOD, CAT, and APX also rose by 109 %, 181 %, and 137 %, respectively, compared to non-primed plants. This nanocomposite application also boosted the levels of phenolics (28 %), ascorbic acid (68 %), total sugars (129 %), flavonoids (39 %), and anthocyanin (29 %) in spinach leaves, while significantly reducing Cd (34.7 %, 53.4 %) and Cr (20.2 %, 28.8 %) contents in plant roots and shoots, respectively. These findings suggest that seed priming with Fe3O4-SiO2 nanocomposites effectively mitigated the toxic effects of Cd and Cr, enhancing the growth and biomass yield of spinach in Cd and Cr co-contaminated environments, offering a promising sustainable approach for producing metal-free crops.

ABSTRACTIt is essential to identify suitable supplements that enhance cell growth, viability, and functional development in cell culture systems. The use of fetal bovine serum (FBS) has been common, but it has limitations, such as batch-to-batch variability, ethical concerns, and risks of environmental contamination. In this study, we explore the potential of Rhodobacter sphaeroides extract, derived from a probiotic photosynthetic bacterium, as an alternative supplement. Our results demonstrate that the extract from R. sphaeroides significantly improves various aspects of cell behavior compared to serum-free conditions. It enhances cell growth and viability to a greater extent than FBS supplementation. Additionally, the extract alleviates oxidative stress by reducing intracellular levels of reactive oxygen species and stimulates lysosomal activity, contributing to cellular processes. The presence of abundant amino acids, glycine and arginine, in the extract may play a role in promoting cell growth. These findings emphasize the potential of R. sphaeroides extract as a valuable supplement for cell culture, offering advantages over the use of FBS.IMPORTANCEThe choice of supplements for cell culture is crucial in biomedical research, but the widely used fetal bovine serum (FBS) has limitations in terms of variability, ethics, and environmental risks. This study explores the potential of an extract from Rhodobacter sphaeroides, a probiotic bacterium, as an alternative supplement. The findings reveal that the R. sphaeroides extract surpasses FBS in enhancing cell growth, viability, and functionality. It also mitigates oxidative stress and stimulates lysosomal activity, critical for cellular health. The extract’s abundance of glycine and arginine, amino acids with known growth-promoting effects, further highlights its potential. By providing a viable substitute for FBS, the R. sphaeroides extract addresses the need for consistent, ethical, and environmentally friendly cell culture supplements. This research paves the way for sustainable and reliable cell culture systems, revolutionizing biomedical research and applications in drug development and regenerative medicine.

Plants are usually provoked by a variety of heavy metal (HM) stressors that have adverse effects on their growth and other biochemical characterizations. Among the HMs, chromium has been considered the most toxic for both plants and animals. The present study was conducted to compare the phytotoxic effects of increasing chromium (VI) salt and nanoparticles (NPs) concentrations on various growth indexes of rice (Oryza sativa L. var. swat 1) seedlings grown in a hydroponic system. The 7-day rice seedlings were exposed to Cr (VI) salt and NPs hydroponic suspensions which were adjusted to the concentration of 0, 50, 100, 150, 200 and 250 mg/L. Both the Cr (VI) salt and NPs with lower concentrations (up to 100mg/L) exerted minimum inhibitory effects on the growth performance of rice seedlings. However, a significant decrease in shoot and root length and their fresh and dry weight was recorded at higher doses of Cr (VI) salt (200 mg/L) and NPs (250 mg/L). The stress induced by Cr (VI) salt has drastically affected the roots, whereas, Cr (VI) NPs significantly affected the shoot tissues. Photosynthetic pigments decreased significantly in a dose-dependent manner, and the reduction was more pronounced in rice seedlings exposed to Cr (VI) NPs compared to Cr (VI) salt. Cr (VI) NPs enhanced the membrane permeability in shoots and roots as compared to that of Cr (VI) salt, which resulted in higher concentration of reactive oxygen species (ROS) and increased lipid peroxidation. The activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) increased significantly in shoot/root tissue following exposure to higher doses of Cr (VI) salt (200 mg/L) and NPs stress (250 mg/L), while minor changes in CAT and APX activities were observed in root and shoot tissues after exposure to higher concentration of Cr (VI) NP. Furthermore, the increasing concentrations of Cr (VI) NPs increased the length of stomatal guard cells. Conclusively, Cr (VI) salt and NPs in higher concentrations have higher potential to damage the growth and induce oxidative stress in rice plants. [ABSTRACT FROM AUTHOR]

Zinc oxide nanoparticles (ZnO NPs) exhibit diverse applications, including antimicrobial, UV-blocking, and catalytic properties, due to their unique structure and properties. This study focused on the characterization of zinc oxide nanoparticles (ZnO NPs) synthesized from Juglans regia leaves and their application in mitigating the impact of simultaneous infection by Meloidogyne arenaria (root-knot nematode) and Macrophomina phaseolina (root-rot fungus) in cowpea plants. The characterization of ZnO NPs was carried out through various analytical techniques, including UV–visible spectrophotometry, Powder-XRD analysis, FT-IR spectroscopy, and SEM-EDX analysis. The study confirmed the successful synthesis of ZnO NPs with a hexagonal wurtzite structure and exceptional purity. Under in vitro conditions, ZnO NPs exhibited significant nematicidal and antifungal activities. The mortality of M. arenaria juveniles increased with rising ZnO NP concentrations, and a similar trend was observed in the inhibition of M. phaseolina mycelial growth. SEM studies revealed physical damage to nematodes and structural distortions in fungal hyphae due to ZnO NP treatment. In infected cowpea plants, ZnO NPs significantly improved plant growth parameters, including plant length, fresh mass, and dry mass, especially at higher concentrations. Leghemoglobin content and the number of root nodules also increased after ZnO NP treatment. Additionally, ZnO NPs reduced gall formation and egg mass production by M. arenaria nematodes and effectively inhibited the growth of M. phaseolina in the roots. Furthermore, histochemical analyses demonstrated a reduction in oxidative stress, as indicated by decreased levels of reactive oxygen species (ROS) and lipid peroxidation in ZnO NP-treated plants. These findings highlight the potential of green-synthesized ZnO NPs as an eco-friendly and effective solution to manage disease complex in cowpea caused by simultaneous nematode and fungal infections.

Seed priming with a composite of iron oxide (Fe 3 O 4) and silicon dioxide (SiO 2) nanoparticles (NPs) is an innovative technique to mitigate cadmium (Cd) and chromium (Cr) uptake in plants from rooting media. The current study explored the impact of seed priming with varying levels of Fe 3 O 4 NPs, SiO 2 NPs, and Fe 3 O 4 -SiO 2 nanocomposites on Cd and Cr absorption and phytotoxicity, metal-induced oxidative stress mitigation, growth and biomass yield of spinach (Spinacia oleracea L.). The results showed that seed priming with the optimum level of 100 mg L−1 of Fe 3 O 4 -SiO 2 nanocomposites significantly (p ≤ 0.05) increased root dry weight (144 %), shoot dry weight (243 %) and leaf area (34.4 %) compared to the control, primarily by safeguarding plant's photosynthetic machinery, oxidative stress and phytotoxicity of metals. Plants treated with this highest level of Fe 3 O 4 -SiO 2 nanocomposites exhibited a substantial increase in photosynthetic and gas exchange indices of spinach plants and enhanced activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) antioxidant enzymes by 45 %, 48 %, and 60 %, respectively. Correspondingly, the relative gene expression levels of SOD, CAT, and APX also rose by 109 %, 181 %, and 137 %, respectively, compared to non-primed plants. This nanocomposite application also boosted the levels of phenolics (28 %), ascorbic acid (68 %), total sugars (129 %), flavonoids (39 %), and anthocyanin (29 %) in spinach leaves, while significantly reducing Cd (34.7 %, 53.4 %) and Cr (20.2 %, 28.8 %) contents in plant roots and shoots, respectively. These findings suggest that seed priming with Fe 3 O 4 -SiO 2 nanocomposites effectively mitigated the toxic effects of Cd and Cr, enhancing the growth and biomass yield of spinach in Cd and Cr co-contaminated environments, offering a promising sustainable approach for producing metal-free crops. [Display omitted] • Seed priming with Fe 3 O 4 -SiO 2 nanocomposite suppressed oxidative stress in spinach plants. • This nanocomposite enhanced the relative gene expression levels of antioxidant enzymes. • It boosted plant biomass by protecting photosynthetic machinery and metal phytotoxicity. • This Fe 3 O 4 -SiO 2 nanocomposite significantly reduced Cd and Cr uptake by plants. [ABSTRACT FROM AUTHOR]