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Aim: Traumatic dental injuries occur frequently in children. Treatment and follow ups are important to avoid short and long term complications. The aim of this study was to investigate the prevalence of sequelae in the permanent anterior teeth following the trauma in their predecessors in the period of 2005 to 2011. Methods: This study was performed retrospectively according the data from the records of 79 children (53 boys, 26 girls), who were treated for anterior deciduous tooth trauma. Of the 79 affected children (186 teeth), 35 (91 teeth, 22 boys and13 girls) were available for the follow-up examination. Kappa coefficient was calculated for inter-examiner reliability. Descriptive statistics including percentage and mean values were also calculated. Results: Sequelae were detected on 45(49.5%) of 91 successors of the traumatized deciduous teeth and enamel hypoplasia and/or discoloration was observed in 14 (31.1%) teeth. Eruption was disturbed in 14 (31.1%). In 13 teeth (28.9%) crown and root malformation, in three teeth (6.7%) only crown and in one tooth (2.2%) only root malformation was detected. Most frequent sequela of successors were observed after the intrusive luxation of the deciduous teeth (33%). Crown-root malformations of the successor teeth were most frequently occurred after the trauma at the age of 0 to 2 (61.53%). Conclusions: Early diagnosis and treatment of primary dental injuries and routine clinical and radiological follow up would minimize the sequelae which were observed in permanent successors..

The aims of this study were to verify the occurrence of dental injuries in 343 amateur Turkish soccer players in Izmir and the level of knowledge of the teams’ soccer players about mouthguards. The soccer players were interviewed to determine the occurrence of dental trauma during soccer and mouthguard usage level. The data were analyzed with descriptive analysis to determine absolute and relative frequencies of answers for each one of the questions. Only 35 (10.2%) soccer players reported the occurrence of some type of dental injury during soccer practice. Regarding emergency conducts, approximately 84 players (24.48%) answered that replantation could be obtained after teeth avulsion, 23 players (27.38%) answered that successful replantation could be obtained within 2 hours immediately after injury, and 60.71% were not able to answer this question. Regarding mouthguard use, 61.8% of soccer players did not know about mouthguards. It was possible to conclude that dental injuries are common during amateur soccer practice and that there is a lack of information in the soccer players related to the emergency conducts and prevention of dental trauma.

Objective: To evaluate the 30-month clinical performance of a nanofilled-resin composite with or without glass-fiber layering in restorations of large cavities in posterior teeth of children. Study Design: A total of 71 restorations were placed in permanent molar teeth of 47 children (mean age 10.9 years) with (FRC; n=35) or without (RC; n=36) fiber layering. One operator placed all restorations. Restorations were evaluated according to the USPHS modified-Ryge criteria at baseline, 6, 12, 18, 24, and 30 months. The data were analyzed using Fisher's exact and chi-square tests and outcomes were compared using the Cochran-Q test (p

WOS: 000269373500012, PubMed ID: 19101739, The aim was to test the null hypotheses that there is no difference: (1) in carious lesion development at the restoration margin between class II composite resin restorations in primary molars produced through the atraumatic restorative treatment (ART) with and without a chemomechanical caries removal gel and (2) in the survival rate of class II composite resin restorations between two treatment groups after 2 years. Three hundred twenty-seven children with 568 class II cavitated lesions were included in a parallel mouth study design. Four operators placed resin composite (Filtek Z 250) restorations bonded with a self-etch adhesive (Adper prompt L pop). Two independent examiners evaluated the restorations after 0.5, 1, and 2 years using the modified Ryge criteria. The Kaplan-Meier survival method was applied to estimate survival percentages. A high proportion of restorations were lost during the study period. Therefore, the first hypothesis could not be tested. No statistically significant difference was observed between the cumulative survival percentages of restorations produced by the two treatment approaches over the 2-year period (ART, 54.1 +/- 3.4%; ART with Carisolv (TM), 46.0 +/- 3.4%). This hypothesis was accepted. ART with chemomechanical gel might not provide an added benefit increasing the survival percentages of ART class II composite resin restorations in primary teeth.

Objective: The microbial quality of water in adental unit water systems (DUWS) is of considerableimportance because patients and dental staff are regularlyexposed to water and aerosol generated from thedental units. The objective of this study was toevaluate the 20 DUWS in general dental practices and todetermine the antibiotic susceptibility of the colonizingbacteria. Methods: Three water and one biofilm samples fromeach DUWS were investigated for total viable count (TVC),oral streptococci, Pseudomonas spp., Enterobacteria,Candida albicans and Legionella pneumophila. Results: Atotal of 44 morphologically different colonies were obtainedfrom water samples and 20 types of colonies (45.5%) couldbe identified using API test strips. The mean TVC valueswere 4.36 log CFU ml )1 for source waters, 4.95 log CFU ml )1 for 3-in-1 syringe samples, 4.91 log CFU ml )1 for air rotorsamples and 3.66 log CFU cm )2 for biofilm samples.Susceptibilities of the isolates were tested against piperacillin,ampicillin, ceftazidime, meropenem, gentamicin, tetracycline,ofloxacin and chloramphenicol by using microdilution methodaccording to NCCLS. The meropenem and ofloxacin haveshown the broadest spectrum against to the testedisolates. Conclusion: The study emphasizes the need foreffective mechanisms to reduce the microbial contaminationin DUWS, and highlights the risk for cross-infection in generaldental practice.Key words: antibiotic susceptibility; biofilm; dental unit watersystems

Objectives: The aim of this study was to investigate the presence of Enterococcus faecalis in endodontic infections in both deciduous and permanent teeth by culture and polymerase chain reaction (PCR) methods.Methods: A total of 145 children aged 5-13 years old were involved in this study. The presence of E. faecalis in necrotic deciduous and permanent teeth root canals was studied using culture and polymerase chain reaction methods.Results: Among 145 molar teeth, 57% (n=83) presented necrotic asymptomatic pulp tissues and were included in this study. Culture and PCR methods detected the test species in 18 and 22 of 83 teeth involved, respectively. E. faecalis was cultured from 8 (18%) of 45 necrotic deciduous teeth and from 10 (26%) of 38 necrotic permanent teeth. PCR detection identified the target species in 10 (22%) and 12 (32%) of necrotic deciduous and permanent teeth respectively. Statistically significant difference in the presence of E. faecalis in deciduous and permanent teeth was found by culture and PCR methods (P=0.03 and 0.02, respectively). The difference in the presence of E. faecalis between two different methods was not statistically significant (P>.05).Conclusions: The results of the present study confirm that both culture and PCR methods are sensitive to detect E. faecalis in root canals. (Eur J Dent 2007;1:216-221)

Background The authors evaluated the 24-month performance of a packable resin-based composite/dentin bonding system and a high-viscosity glass ionomer cement (GIC) in restorations placed in primary molars with the atraumatic restorative treatment (ART) approach. Methods Three dentists placed 419 restorations in 219 children aged 6 through 10 years who had bilateral matched pairs of carious posterior Class I and II primary teeth. They used a split-mouth design to place the two materials, which were assigned randomly to contralateral sides. The authors evaluated the restorations according to U.S. Public Health Service Ryge criteria. Results After 24 months, 96.7 percent of the Class I GIC restorations and 91 percent of the resin-based composite restorations survived, while the success rates for the Class II restorations were 76.1 percent and 82 percent for the GIC and resin-based composite restorations, respectively. The survival rate of the Class II resin-based composite restorations was 5.9 percent higher than that of the GIC restorations at the 24-month evaluation, but this difference was not statistically significant. However, the study results showed a statistically significant difference in survival rates between Class I and II restorations for both materials. Conclusion and Clinical Implications The two-year clinical performance of both materials was satisfactory for the restoration of Class I and II primary molars using the ART approach.

The aim of this study was to evaluate the in vivo efficacy of three intracanal medicaments (Ca(OH)2, 1% chlorhexidine gel and 1% chlorhexidine gel with Ca(OH)2 against Enterococcus faecalis in necrotic primary teeth. As a conclusion, chlorhexidine gel with or without Ca(OH)2 was more effective than Ca(OH)2 alone against Enterococcus faecalis.

Aim To compare the antibacterial properties and toxicity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate and 0.2% chlorhexidine gluconate plus 0.2% cetrimide (Cetrexidin®; Vebas, San Giuliano, Milan, Italy). Methodology The antibacterial effects of the irrigants in vitro were examined after 5 min and 48 h in freshly extracted human teeth with single roots, whose canals were infected by Enterococcus faecalis ATCC 29212. In a separate in vivo study, bacterial culture samples were collected before treatment from the infected root canals of deciduous teeth containing necrotic pulp tissue. Irrigants were used to clean the canals which were then left empty for 48 h. Aerobic/facultative anaerobic and anaerobic bacterial growth were compared before and 48 h after irrigation. Finally, the toxic effects of the irrigants were assessed by injecting them into the subcutaneous tissues of rats. The inflammatory reactions that occurred 2 h, 48 h and 2 weeks after the injections were evaluated. Results In the laboratory study, the 2% chlorhexidine gluconate and Cetrexidin® were significantly more effective on E. faecalis than the 5.25% NaOCl at 5 min (P

The purpose of this study was to determine the cytotoxic effects of five different primary tooth root canal filling materials on L929 permanent cell line with MTT assay.Kri 1 paste (iodoform), Diapex (iodoform+Ca(OH)(2)), Metapaste (Ca(OH)(2) with distilled water), Dentalis (iodoform+ZOE+Ca(OH)(2)) and Kalsin (Ca(OH)(2) with glycerin) were used in this study. Tested materials were in contact for 24, 48 and 72 hours with L929 cells. At the end of the test periods, MTT test solutions were added to the plates and incubated for 3 hours at 37 degrees C. Then optic densities were read using UV visible spectrophotometer. All assays were repeated three times to ensure reproducibility. The obtained data were analyzed statistically by one-way analysis of variance and Dunnett T3 post hoc test (P0.05).All tested materials were found cytotoxic on L929 cell line. It was found that Kri 1 paste group showed the highest survival rates.We concluded that the use of Kri 1 paste as a root canal filling material is a better option than other medications in primary teeth. Further research is necessary to determine the effect of root canal filling materials on vital tissues.

Kabuki Syndrome (KS) is a multiple congenital anomalies/mental retardation syndrome of unknown etiology. It is characterized by a dysmorphic face, postnatal growth retardation, skeletal abnormalities, mental retardation, and unusual dermatoglyphic patterns. The characteristic dental findings include hypodontia, microdontia, absence of upper lateral and lower central incisors and upper molars, abnormal tooth shape, widely spaced teeth, ectopic upper molars and malocclusion. The purpose of this report is to describe common and additional dental findings and dental treatment of an 11-year old female patient with KS. This case report emphasises the importance of oral and dental manifestations for diagnosis.

Background. The aim of this study was to assess the incidence of bacteremia from various procedures in children undergoing dental treatment under general anesthesia. Methods. Three blood samples for cultures were obtained from each patient; the first sample (10 ml basal) at the onset of the process and the second sample (10 ml) within 30 seconds following the nasotracheal intubation. The third blood sample (10 ml) was taken 30 seconds after the extraction of a deciduous tooth or a permanent tooth or slow drilling. Following incubation in an automated blood culture system, bacteria were identified by using conventional biochemical methods and commercial identification systems. Chi-square test was used to assess the findings statistically. Results. All blood cultures were negative before intubation (baseline) in every patient. However, the percentage of positive samples in deciduous tooth extraction and permanent tooth extraction groups were 18/26 (69.2%) and 18/25 (72%), respectively. These values were significantly greater than the results of baseline 0/74 (0%), slow drilling 3/23 (13%), and nasotracheal intubation 9/74 (12.3%) (P

The aim of this study was to compare the serum immunoglobulin A (IgA) and immunoglobulin G (IgG) levels and the presence of Treponema denticola in the root canals in a group of teeth with/without periapical lesion. A total of 66 children aged 8 to 13 years old were involved in this study. Five milliliters of blood samples were taken to detect the serum IgA and IgG levels. Sixty-six endodontic samplings were also obtained to determine the presence of T. denticola by polymerase chain reaction. The presence of T. denticola between the groups with/without periapical lesion was statistically significant (p = 0.026). A significant negative correlation was found between serum IgG and IgA levels and the presence of T. denticola (p = 0.023 and 0.038, respectively). This study may support the hypothesis that the presence of T. denticola in the root canals is mainly related to the periapical lesions, and the higher levels of serum IgG and IgA levels may protect against T. denticola.

The talon cusp is a relatively rare dental developmental anomaly characterized by the presence of an accessory cusp-like structure projecting from the cingulum area or cementoenamel junction. The alteration can cause clinical problems such as caries or occlusal interference. Management of the talon cusp varies according to the circumstances of the individual case and should be as conservative as possible. The purpose of this report was to present the case of bilateral talon cusp in primary maxillary central incisors that was successfully managed with conservative therapy.

This study sought to compare the antibacterial efficacy of three commonly used intracanal medicaments with propolis against Enterococcus faecalis. This study utilized 180 freshly extracted single-rooted intact human permanent teeth with a single root canal. After root canal preparations and sterilization, canals were contaminated with E. faecalis and incubated at 37 degrees C (+/- 1.0 degrees C) for seven days. The teeth were divided randomly into six groups. To determine bacterial growth on blood agar, microbiological samples were carried out with sterile paper points to evaluate results at 48 hours and at ten days. All data were analyzed statistically with t-test, Mann Whitney, Kruskal Wallis, and one-way ANOVA tests. This study revealed that propolis had good in vitro antibacterial activity against E. faecalis in the root canals, suggesting that it could be used as an alternative intracanal medicament.

The objective of this study was to compare the postoperative morbidities for 24 hours following dental care under day-stay general anesthesia using sevoflurane or halothane in intellectually disabled children.Eighty-six premedicated patients with intellectual disabilities underwent general anesthesia for their dental treatment. They were randomly given anesthesia maintained with sevoflurane (2% to 3%) or halothane (1% to 1.5%) after receiving inhalation induction either with sevoflurane (8%) or halothane (5%) and nitrous oxide in oxygen (50:50). The patients' age, gender, weight, ASA Class, type of dental treatment, and duration of anesthesia and operation were recorded as well as the time required for recovery (Aldrete Scale) and the length of time taken before they were discharged (postanesthetic discharge scoring system) from the hospital. Pain and agitation were recorded using a visual analog scale (0 to 10). Other postoperative morbidities, which include crying, nausea and vomiting, bleeding, and drowsiness, were also noted for 24 hours after the operation.The most common morbidities during the postoperative 24 hours were agitation and pain, and their occurrence was significantly more common in the sevoflurane group than in the halothane group (P.05). The recovery time was shorter in the sevoflurane group, but it was not statistically significant. There was no difference between the groups in the discharge time.Apart from more postoperative agitation and pain after awakening from sevoflurane, the quality of recovery was similar for both sevoflurane and halothane.

PubMed ID: 15881836, Propolis means a gum that is gathered by bees from various plants. It is known for its biological properties, having antibacterial, antifungal and healing properties. The aims of this study were to evaluate the antimicrobial activity of four different Anatolian propolis samples on different groups of microorganisms including some oral pathogens and comparison between their chemical compositions. Ethanol extracts of propolis (EEP) were prepared from four different Anatolian propolis samples and examined whether EEP inhibit the growth of the test microorganisms or not. For the antimicrobial activity assays, minimum inhibitory concentrations (MIC) were determined by using macrodilution method. The MIC values of the most effective propolis (TB) were 2 µg/ml for Streptococcus sobrinus and Enterococcus faecalis, 4 µg/ml for Micrococcus luteus, Candida albicans and C. krusei, 8 µg/ml for Streptococcus mutans, Staphylococcus aureus, Staphylococcus epidermidis and Enterobacter aerogenes, 16 µg/ml for Escherichia coli and C. tropicalis and 32 µg/ml for Salmonella typhimurium and Pseudomonas aeruginosa. The chemical compositions of EEP's were determined by high-temperature high-resolution gas chromatography coupled to mass spectrometry. The main compounds of four Anatolian propolis samples were flavonoids such as pinocembrin, pinostropin, isalpinin, pinobanksin, quercetin, naringenin, galangine and chrysin. Although propolis samples were collected from different regions of Anatolia all showed significant antimicrobial activity against the Gram positive bacteria and yeasts. Propolis can prevent dental caries since it demonstrated significant antimicrobial activity against the microorganisms such as Streptococcus mutans, Streptococcus sobrinus and C. albicans, which involves in oral diseases. © 2005 Elsevier GmbH. All rights reserved.

Fusion has been described as a developmental anomaly characterized by the union of 2 adjacent teeth. Bilateral dental fusion in the primary dentition is a rare dental anomaly. The purpose of this report was to present a 9-year-old boy who had bilateral fusion of his mandibular primary lateral incisor and canine teeth.