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In this study, the cytotoxic effects of the extracts of methanol: water (80:20)prepared from the above-ground parts of the varieties of Onobrychis albifloraHub.-Mor., Onobrychis argyrea Boiss. subsp. argyrea, Onobrychis galegifoliaBoiss. and Onobrychis tournefortii (Willd.) Desv. species to HCT-116 cellswere investigated. With cytotoxicity analysis that with these species inhibitorconcentrations (IC50) which resulted in a 50% reduction in the proliferation ofHCT-116 cells were identified. In continuation of the study; the antiproliferativeand apoptotic effects of Onobrychis albiflora extract on HCT-116 cells wereevaluated by Caspase 3, Annexin V / PI Apoptosis / Necrosis analysis, ApopxinGreen and 7-AAD Apoptosis / Necrosis analysis. The antiproliferative, apop-totic and necrotic effects of Onobrychis albiflora extract on HCT-116 cells alsowere determined.

The bioactive compounds of detrital leaves of seaweed, Zostera spp. found as beach-cast were investigated by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Extracts of methanol, ethyl acetate, and n-hexane of detrital Zostera spp. leaves collected from Coasts of Aegean Sea, Izmir (Turkey) were used to determine the major bioactive constituents. Major bioactive elements in detrital leaves of Zostera spp. were elucidated as apigenin-7-O-glucoside, luteolin-7-O-glucoside, rosmarinic acid and diosmetin-7-sulfate. Minor amounts of apigenin-7-O-glucoside, luteolin-7-O-glucoside and rosmarinic acid were identified in the detrital leaves. Diosmetin-7-sulfate was the major compound in quantity. The antimicrobial activities of the extracts and individual bioactive compounds were tested for Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Candida albicans and Vibrio alginolyticus. The antimicrobial activity of solvent extracts and the isolated compunds exhibited different sensitivities to the microorganisms tested. Antimicrobial activity was higher in the ethyl acetate extract in comparison to n-hexane and methanol extracts. Diosmetin-7-sulfate had no antimicrobial activity. Apigenin-7-O-glucoside, luteolin-7-O-glucoside and rosmarinic acid inhibited antifungal growth. Apigenin-7-O-glucoside and luteolin-7-O-glucoside showed antibacterial activity with different sensitivity towards bacteria. Dead biomass of Zostera spp. in beach-cast form can be used as a source for bioactive constituents. The bioactive potential of 'detrital leaves' of Zostera spp. may be of importance in projecting towards various biological applications.

WOS: 000395523800024, PubMed ID: 27978496, Alchemilla mollis (Buser) Rothm aerial part and root methanolic-water extracts were evaluated for their hepatoprotective activity on carbon tetrachloride induced hepatotoxicity and hypoglycemic activity on alloxan-induced diabetic mice. None of the tested extracts exhibited effects on blood glucose levels. However hepatoprotective activity results have revealed that serum ALT levels were significantly lowered by both the aerial part and root extracts at doses of 100 mg/kg and 200 mg/kg. Histopathological examination showed that A. mollis aerial parts and roots induced significant recovery from cellular damage; when compared to the carbon tetrachloride group, the most significant activity was observed with A. mollis aerial part extracts at a dose of 200 mg/kg. There is evidence of a hepatoprotective activity of A. mollis on the phenolic content of the plant, especially in the case of flavonoids, which have potent antioxidant properties. (C) 2016 Elsevier Masson SAS. All rights reserved.

WOS: 000359380100025, PubMed ID: 25700659, The aim of the present study is to evaluate the treatment potential of Alchemilla mollis (Buser) Rothm. and Alchemilla persica Rothm. in the experimentally induced endometriosis model in rats. Endometriosis was surgically induced in rats by autotransplanting endometrial tissue to abdominal wall. Thirty-six rats were randomly divided into six groups. The groups were orally treated with the methanol:water (80:20) extracts of aerial parts and roots of A. mollis and A. persica. Buserelin acetate (20 mg) was used as the reference drug. The phytochemical contents of the most active extracts were determined by high performance liquid chromatography. The cystic formation was determined to be significantly decreased with the aerial part extract of A. mollis. A reduction in the endometrioma was also determined for the aerial part extract of A. persica group. However, significant reduction on the levels of cytokine were recorded for the A. mollis aerial part extract group. Therefore, the phytochemical contents of the aerial part extracts of A. mollis. and A. persica were analyzed. The results of the present study revealed that the aerial part extracts of A. mollis and A. persica could be beneficial in the treatment of endometriosis.