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Additional file 5. Mitochondria are distributed between astrocytes via tunneling nanotubes. Arrows indicate transfer of MitoTracker™ Green labeled mitochondria from the top cell to the bottom cell via tunneling nanotubes. The top panel show fluorescent mitochondria in white and the bottom panel show the merged images (GFP and bright field) of the same time points.

Additional file 3. Mitochondrial fusion protein levels are not affected by Aβ exposure. Western blot analysis demonstrated that OPA1 levels remain unchanged by Aβ exposure at 7d and 7d+6d.

Additional file 8. Lipid droplets are found in both Aβ exposed and control astrocytes. Lipid droplets (white asterisks) are observed in Aβ exposed astrocytes (a,b) and in controls (c,c´).

Additional file 1. Astrocytic markers. hiPSC-derived astrocytes were stained with DAPI (blue) and the astrocytic markers: GLAST-1 (A), Vimentin (B) S100β (C), AQP4 (D), GFAP (E), and Nestin (F). Scale bar: 20 μm.

Additional file 2. Aβ exposure affects mitochondrial motility in astrocytes. The parameter displacement, distance, velocity and speed were analyzed with Mitometer (A). Motility analysis showed a significant increase in the mitochondrial speed (B) and velocity (C) in Aβ-exposed astrocytes.

Additional file 7. Aβ exposure does not affect basal respiration of mitochondria, cell viability or apoptosis in astrocytes. Seahorse OCR analysis showed no differences in mitochondrial basal respiration between control and Aβ-exposed astrocytes (A). Alamar blue assay showed no decrease in viability in the astrocyte cultures exposed to Aβ (B). Similarly, WB analysis showed no increase in apoptosis markers caspase-3 and BAX in the Aβ-exposedcultures (C and D).