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21 results on '"Pérez-Parallé, M. Luz"'

1. Light pollution in shallow coastal waters: aquaculture farming in the Galician Atlantic shoreline

Author

Bará, Salvador, Pérez-Parallé, M. Luz, Sánchez, José L., Lima, Raul Cerveira, Cegarra, Xurxo, and Bouzas, José Antonio Fernández

Subjects
Physics - Atmospheric and Oceanic Physics
Abstract

Artificial light at night is a relevant environmental stressor whose unwanted consequences have been documented by a growing body of research. In this work we analize the loss of the natural darkness of the night in aquaculture farms located in shallow waters close to the shoreline, in the Atlantic coast of Galicia. High-luminance LED lampposts are switched-on the whole night long for surveillance purposes, significantly increasing the light levels throughout the whole water column, from the surface to the seabed. Measured in terms of the weighted radiance associated with the significant magnitude m1/3, the zenithal brightness at these locations is 125 times higher than should correspond to a pristine natural site. Given the potential severity of the environmental effects of artificial light at night, the dubious efficacy of light per se as a theft deterrence factor, and the availability of more efficient and environmentally friendly technologies for surveillance, the use of permanent floodlight sources in coastal areas is strongly discouraged and should be restricted as much as possible., Comment: ArXiv number added, otherwise identical to version 1

Published
2018

9. RNA-Seq Transcriptome Profiling of the Queen Scallop (Aequipecten opercularis) Digestive Gland after Exposure to Domoic Acid-Producing Pseudo-nitzschia

Author

Ventoso, Pablo, Pazos, Antonio J., Pérez-Parallé, M. Luz, Blanco, Juan, Triviño, Juan C., and Sánchez, José L.

Subjects
scallop, qPCR, domoic acid, lcsh:R, oxidative stress, lcsh:Medicine, Aequipecten opercularis, bivalves, RNA-seq, transcriptome, differential expression, amnesic shellfish poisoning (ASP)
Abstract

Some species of the genus Pseudo-nitzschia produce the toxin domoic acid, which causes amnesic shellfish poisoning (ASP). Given that bivalve mollusks are filter feeders, they can accumulate these toxins in their tissues. To elucidate the transcriptional response of the queen scallop Aequipecten opercularis after exposure to domoic acid-producing Pseudo-nitzschia, the digestive gland transcriptome was de novo assembled using an Illumina HiSeq 2000 platform. Then, a differential gene expression analysis was performed. After the assembly, 142,137 unigenes were obtained, and a total of 10,144 genes were differentially expressed in the groups exposed to the toxin. Functional enrichment analysis found that 374 Pfam (protein families database) domains were significantly enriched. The C1q domain, the C-type lectin, the major facilitator superfamily, the immunoglobulin domain, and the cytochrome P450 were among the most enriched Pfam domains. Protein network analysis showed a small number of highly connected nodes involved in specific functions: proteasome components, mitochondrial ribosomal proteins, protein translocases of mitochondrial membranes, cytochromes P450, and glutathione S-transferases. The results suggest that exposure to domoic acid-producing organisms causes oxidative stress and mitochondrial dysfunction. The transcriptional response counteracts these effects with the up-regulation of genes coding for some mitochondrial proteins, proteasome components, and antioxidant enzymes (glutathione S-transferases, thioredoxins, glutaredoxins, and copper/zinc superoxide dismutases).

Published
2019

19. Eight-hour PCR-based procedure for the detection of Salmonella in raw oysters

Author

Vázquez-Novelle, M. Dolores, Pazos, Antonio J., Abad, Marcelina, Sánchez, José L., and Pérez-Parallé, M. Luz

Subjects
DETECTION of microorganisms, SALMONELLA, POLYMERASE chain reaction, OYSTERS
Abstract

Abstract: The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isothiocyanate, E.Z.N.A. Mollusc Kit, Chelex®-100, and lysis with detergents) were compared. A nested PCR method combined with 3.5 h pre-enrichment in buffered peptone water (BPW) and DNA extraction by the resin Chelex®-100 is proposed for the detection of S. senftenberg in oyster samples. The detection limit of the method is less than 0.1 CFU/ml (<1 CFU/g of oyster). This procedure is shown to be an excellent tool for the sensitive detection of S. senftenberg from naturally contaminated oysters, with results being obtained within 8 h. [Copyright &y& Elsevier]

Published
2005
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20. Effects of food ration, water flow rate and bacteriological levels of broodstock on the reproductive conditioning of the European flat oyster (Ostrea edulis,Linnaeus 1758)

Author

Maneiro, Verónica, Santos, Ysabel, Pazos, Antonio J., Silva, Arturo, Torres-Corral, Yolanda, Sánchez, José L., and Pérez-Parallé, M. Luz

Abstract

•Oysters conditioned with a food ration of 3 % produced the largest number of larvae.•Oysters conditioned at flow rate of 3 L h−1oyster−1had the highest mortality rate.•Highest levels of vibrio and heterotropic bacteria were found at 3 L h−1oyster−1.•Best larval production obtained at 3% food ration and 2 L h−1oyster−1flow rate.•Influence of bacteriological charge of broodstock is studied for the first time.

Published
2020
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