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Your search keyword '"Pérez-Salcedo L"' showing total 11 results
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11 results on '"Pérez-Salcedo L"'

1. Molecular identification of black-pigmented bacteria from subgingival samples of cats suffering from periodontal disease

Author

Pérez-Salcedo, L., Laguna, E., Sánchez, M. C., Marín, M. J., O`Connor, A., González, I., Sanz Alonso, Mariano, Herrera González, David, Pérez-Salcedo, L., Laguna, E., Sánchez, M. C., Marín, M. J., O`Connor, A., González, I., Sanz Alonso, Mariano, and Herrera González, David

Abstract

Objectives: To characterise the black-pigmented bacterial species found in the subgingival samples of cats with periodontal disease using molecular-based microbiological techniques. Methods: Sixty-five subgingival samples obtained from 50 cats with periodontal disease were analysed by polymerase chain reaction amplified ribosomal DNA restriction analysis and cloning and sequencing of the 16S rRNA genes. Results: Among the 65 subgingival samples, eight phylogenetic profiles were obtained, of which the most prevalent species were: Porphyromonas gulae (40%), P. gingivalis/P. gulae (36 · 9%), P. gulae/Porphyromonas sp. UQD 406 (9 · 2%), Odoribacter denticanis (6 · 2%), P. gulae/Porphyromonas sp. UQD 348 (1 · 5%) and P. circumdentaria (1 · 5%). When compared with the species resulting from biochemical diagnosis, the identification of P. gulae was congruent in 70% of the cases, while colonies identified as P. intermedia-like corresponded in 80% of cases to P. gulae. Clinical significance: The use of molecular-based microbiological diagnostic techniques resulted in a predominance of Porphyromonas spp. in the subgingival plaque of cats suffering from periodontal disease. Further characterisation of these bacteria identified P. gulae, O. denticanis and P. circumdentaria. The more frequently detected phylogenetic profiles corresponded to P. gingivalis and P. gulae., Depto. de Especialidades Clínicas Odontológicas, Fac. de Odontología, TRUE, pub

Published
2015

2. Colgajo de reposición apical

Author

Pérez-Salcedo, L. and Bascones Martínez, A.

Subjects
Colgajo de reposición apical, Apically positioned flap
Abstract

Se describe la técnica del colgajo de reposición apical desde el primer autor, Nabers en 1954, así como las modificaciones realizadas por Ariaudo y Tyrrel en 1957 y Friedman en 1962. También se presenta un caso clínico donde se describen los diferentes pasos de la cirugía de reposición apical. Por último, se hace una revisión evaluando la eficacia del colgajo de reposición apical en el tratamiento de la periodontitis. This paper describes the technique apically positioned flap since the first author Nabers in 1954 and also the modifications performed by Ariaudo and Tyrrell in 1957 and Friedman in 1962. Also a clinical case is presented where it can be observed the different steps of the apically positioned flap in clinical pictures. Afterwards some papers in which the efficacy of the apically positioned flap in the treatment of periodontitis are evaluated and described.

Published
2011

4. Tumores benignos de la mucosa oral

Author

Pérez-Salcedo, L. and Bascones Martínez, A.

Subjects
Tumor benign, surgical excision, escisión quirúrgica, Benign tumor
Abstract

La mayoría de los tumores de los tejidos blandos pueden presentarse en varios sitios del organismo, así como en la mucosa bucal. En este artículo se van a describir los diferentes tipos de tumores benignos de la mucosa oral, se clasificarán según el tejido de procedencia. El diagnóstico definitivo será histológico y permitirá tomar una decisión terapéutica. The majority of soft tissues tumors can be present in various sites of the organism, as well as in the oral cavity. In this paper, we will describe all the different types of benign tumors in the oral cavity, the classification will be according to the tissue of origin. The definitive diagnosis would be histological and this would provide the correct treatment.

Published
2010

10. Isolation and Identification of Porphyromonasspp. and other Putative Pathogens from Cats with Periodontal Disease

Author

Pérez-Salcedo, L., Herrera, D., Esteban-Saltiveri, D., León, R., Jeusette, I., Torre, C., O'Connor, A., González, I., and Sanz, M.

Abstract

The purpose of this study was to evaluate the subgingival microbiota and determine the most prevalent periodontal pathogens implicated in feline periodontal disease and to correlate these findings with the clinical periodontal status. Subgingival microbiological samples were taken under sedation from 50 cats with clinical signs of periodontal disease. Pooled paper point samples from 4 selected subgingival sites were cultured on blood agar and on Dentaid-1 medium. Suspected pathogens were identified, subcultured, and preserved. The association between the microbiological findings and the clinical status was studied using correlation coefficients (CC). In addition, cats were stratified in subgroups according to presence of putative pathogens, and comparisons were carried out using unpaired t-test. Three bacterial species were frequently detected including Porphyromonas gulae (86 %), Porphyromonas circumdentaria (70 %) and Fusobacterium nucleatum (90 %). The mean proportion of total flora was high for P. gulae (32.54 %), moderate for P. circundentaria (8.82 %), and low for F. nucleatum (3.96 %). Among the clinical variables, tooth mobility was correlated (CC > 0.50, p < 0.001) with recession, pocket depth, attachment level, gingival index, and calculus index (CC = 0.29, p = 0.04) as well as with total bacterial counts (CC = 0.38, p = 0.006). Cats with more than 10 % of P. gulae showed significantly more mobility (p = 0.014) and recession (p = 0.038), and a tendency for deeper probing pocket depths (p = 0.084) and attachment loss (p = 0.087). The results from this cross-sectional study confirmed that P. gulae is the most relevant pathogen in periodontal disease in cats.

Published
2013
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11. 24-hour evaluation of dental plaque bacteria and halitosis after consumption of a single placebo or dental treat by dogs.

Author

Jeusette IC, Román AM, Torre C, Crusafont J, Sánchez N, Sánchez MC, Pérez-Salcedo L, and Herrera D

Subjects
Animals, Dogs, Halitosis drug therapy, Real-Time Polymerase Chain Reaction, Sulfur Compounds, Bacteria drug effects, Bacterial Load drug effects, Dental Plaque microbiology, Dog Diseases drug therapy, Halitosis veterinary
Abstract

OBJECTIVE To determine whether consumption of a single dental treat with specific mechanical properties and active ingredients would provide a 24-hour effect on dental plaque bacteria and halitosis in dogs. ANIMALS 10 dogs of various breeds from a privately owned colony that had received routine dental scaling and polishing 4 weeks before the study began. PROCEDURES Dogs were randomly assigned to receive 1 placebo or dental treat first. A 4-week washout period was provided, and then dogs received the opposite treatment. Oral plaque and breath samples were collected before and 0.5, 3, 12, and 24 hours after treat consumption. Volatile sulfur compounds (VSCs) concentration was measured in breath samples. Total aerobic, total anaerobic, Porphyromonas gulae, Prevotella intermedia-like, Tannerella forsythia, and Fusobacterium nucleatum bacterial counts (measured via bacterial culture) and total live bacterial counts, total live and dead bacterial counts, and bacterial vitality (measured via quantitative real-time PCR assay) were assessed in plaque samples. RESULTS Compared with placebo treat consumption, dental treat consumption resulted in a significant decrease in breath VSCs concentration and all plaque bacterial counts, without an effect on bacterial vitality. Effects of the dental treat versus the placebo treat persisted for 12 hours for several bacterial counts and for 24 hours for breath VSCs concentration. CONCLUSIONS AND CLINICAL RELEVANCE Although clinical benefits should be investigated in larger scale, longer-term studies, results of this study suggested that feeding the evaluated dental treat may help to decrease oral bacterial growth in dogs for 12 hours and oral malodor for 24 hours. A feeding interval of 12 hours is therefore recommended.

Published
2016
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