396 results on '"P. Di Bartolo"'
Search Results
2. From pediatric to adult care: a survey on the transition process in type 1 diabetes mellitus and the diabetes services in Italy
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Graziani, Vanna, Suprani, Tosca, Di Bartolo, Paolo, and Marchetti, Federico
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- 2024
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3. Temporal trends in the starting of insulin therapy in type 2 diabetes in Italy: data from the AMD Annals initiative
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Giandalia, A., Nicolucci, A., Modugno, M., Lucisano, G., Rossi, M. C., Manicardi, V., Rocca, A., Di Cianni, G., Di Bartolo, P., Candido, R., Cucinotta, D., and Russo, G. T.
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- 2024
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4. Biderivations of complete Lie algebras
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Di Bartolo, Alfonso and La Rosa, Gianmarco
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Mathematics - Rings and Algebras ,17B40, 17B05, 17B20 - Abstract
The authors of this article intend to present some results obtained in the study of biderivations of complete Lie algebras. Firstly they present a matricial approach to do this, which was a useful and explanatory tool not only in the study of biderivations but also in the synthesis of these results. Then they study all biderivations of a Lie algebra $L$ with $\operatorname{Z}(L)=0$ and $\operatorname{Der(L)}=\operatorname{ad(L)}$, called complete. Moreover, as an application of the previous result, they describe all biderivations of a semisimple Lie algebra (that are complete), extending a result obtained by X. Tang in ([20]) that describes all biderivations of a complex simple Lie algebra. And thirdly, results on symmetric and skew-symmetric biderivations are also presented., Comment: Accepted manuscript to appear in JAA
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- 2023
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5. Non-nilpotent Leibniz algebras with one-dimensional derived subalgebra
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Di Bartolo, Alfonso, La Rosa, Gianmarco, and Mancini, Manuel
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Mathematics - Rings and Algebras ,16W25, 17A32, 17B30, 17B40, 20M99, 22A30 - Abstract
In this paper we study non-nilpotent non-Lie Leibniz $\mathbb{F}$-algebras with one-dimensional derived subalgebra, where $\mathbb{F}$ is a field with $\operatorname{char}(\mathbb{F}) \neq 2$. We prove that such an algebra is isomorphic to the direct sum of the two-dimensional non-nilpotent non-Lie Leibniz algebra and an abelian algebra. We denote it by $L_n$, where $n=\dim_{\mathbb{F}} L_n$. This generalizes the result found in [11], which is only valid when $\mathbb{F}=\mathbb{C}$. Moreover, we find the Lie algebra of derivations, its Lie group of automorphisms and the Leibniz algebra of biderivations of $L_n$. Eventually, we solve the coquecigrue problem for $L_n$ by integrating it into a Lie rack., Comment: Final version, accepted for publication
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- 2023
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6. Quality of care and clinical inertia in the management of cardiovascular risk factors in patients with type 1 and type 2 diabetes: data from AMD annals
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Da Porto, A., Candido, R., Rocca, A., Manicardi, V., Nicolucci, A., Miranda, C., Cimino, E., Di Bartolo, P., Di Cianni, G., and Russo, G.
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- 2024
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7. Non-Nilpotent Leibniz Algebras with One-Dimensional Derived Subalgebra
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Di Bartolo, Alfonso, La Rosa, Gianmarco, and Mancini, Manuel
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- 2024
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8. Experiences, behaviours, and perspectives of young cancer survivors on physical activity
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Ewan Thomas, Luca Di Bartolo, Marina Galioto, Domiziana Seminara, Susanna Pusa, Rebecca Baxter, Sonia Ortega-Gómez, David Jiménez-Pavón, Maria Vasilopoulou, Apostolos Vantarakis, Paula Tavares, Maria João Campos, Petra Thaller, Joshua Thaller, Sofia Papakonstantinou, Musa Kirkar, Sara Vivirito, Francesca Glorioso, Ennio Iannitto, Mario Lo Mauro, and Antonino Bianco
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Exercise ,Family nursing ,Social support ,Healthy lifestyle ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
This narrative review aimed to identify young cancer survivors’ behaviours, experiences, and perspectives concerning physical activity, and identify useful strategies for promoting a healthy lifestyle. A manual search on the following databases was conducted: PubMed, Scopus, and Web of Science. The search was conducted between June 1, 2023, and April 12, 2024. Articles published from database inception up to April 12, 2024, were retrieved. Articles published in any language were considered. Perspectives including ideas, perceived barriers, and facilitators have been identified. Young cancer survivors seem to engage in physical activity as a useful coping strategy to regain normality and keep healthy after the cancer diagnosis. Although emotional and social support seems fundamental to increase participation, several other factors, including physical limitations, fatigue, sex, cancer type, and socio-economic status can influence physical activity participation. For those engaged in physical activity, the preferred activities are walking, biking, going to the gym, and exercising at home, while the least preferred are exercising at the hospital or boot camp-based exercises. Yoga is more frequently chosen by those still under treatment. Young cancer survivors appear to have unique needs different from those of adult cancer survivors. Mode of treatment delivery, increased awareness concerning the effects of physical activity, including families and friends, connecting survivors, and providing social support together with increasing motivation are key strategies for the promotion of physical activity in young cancer survivors. Fitness and healthcare professionals should consider these aspects to increase young cancer survivors' involvement in physical activities.
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- 2024
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9. Gut microorganism enzymes unlock universal blood
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Satchwell, Timothy J., Di Bartolo, Natalie, and Toye, Ashley M.
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- 2024
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10. Corrigendum: Biosecurity measures to control hepatitis E virus on European pig farms
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Tamino Dubbert, Marina Meester, Richard Piers Smith, Tijs J. Tobias, Ilaria Di Bartolo, Reimar Johne, Enrico Pavoni, Gergana Krumova-Valcheva, Elena Lucia Sassu, Christopher Prigge, Giuseppe Aprea, Hannah May, Nadine Althof, Giovanni Ianiro, Jacek Żmudzki, Albena Dimitrova, Giovanni Loris Alborali, Daniela D'Angelantonio, Silvia Scattolini, Noemi Battistelli, and Elke Burow
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hepatitis-E-virus ,HEV ,biosecurity measures ,pig production ,BIOPIGEE ,One Health ,Veterinary medicine ,SF600-1100 - Published
- 2024
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11. Effects of resistance training on sleep quality and disorders among individuals diagnosed with cancer: A systematic review and meta‐analysis of randomized controlled trials
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Dora Maric, Salvatore Ficarra, Luca Di Bartolo, Carlo Rossi, Zoi Asimakopoulou, Apostolos Vantarakis, Ana Carbonell‐Baeza, David Jiménez‐Pavón, Beatriz Gomes, Paula Tavares, Rebecca Baxter, Susanna Pusa, Petra Thaller, Sofia Papakonstantinou, Musa Kirkar, Francesca Glorioso, Marina Galioto, Ambra Gentile, Ewan Thomas, and Antonino Bianco
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exercise oncology ,insomnia ,physical activity ,quality of life ,tumor ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Sleep disorders are often complained by cancer patients and can last years after the end of therapies, leading to different negative consequences. Non‐pharmacological strategies such as exercise interventions may be considered to counteract this phenomenon. The literature supports the beneficial effects of aerobic training (AT), while evidence on resistance training (RT) is scarce. Accordingly, our systematic review aims to investigate the potential novel effect of RT on sleep outcomes in cancer survivors. Methods The literature search was conducted on MEDLINE (Pubmed), Web of Science, Scopus, and Cochrane Central Register of Controlled Trials databases, including only randomized controlled trials (RCTs). The screening procedure was conducted using the web‐based software COVIDENCE. Sleep outcomes assessed through self‐reported questionnaires or objective sleep measurements were extracted from RCTs recruiting cancer survivors of any age and gender, on or off treatment. The risk of bias (RoB) for each study was assessed using the Cochrane RoB 2 tool for RCTs. Meta‐analytic syntheses were performed on sleep quality and insomnia. Results A total of 21 studies were included in the review. Considering the mean percentage differences of all studies combined, promising positive results were found after combined aerobic and resistance exercise program (COMB) for sleep quality (−19%) and sleep disturbance (−17.3%). The meta‐analysis results showed significant improvement for both sleep quality and insomnia (d = 0.28, SE: 0.11, Z = 2.51, p
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- 2024
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12. Investigating the Hepatitis E Virus (HEV) Diversity in Rat Reservoirs from Northern Italy
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Luca De Sabato, Marina Monini, Roberta Galuppi, Filippo Maria Dini, Giovanni Ianiro, Gabriele Vaccari, Fabio Ostanello, and Ilaria Di Bartolo
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hepatitis E virus ,reservoir ,rat ,zoonoses ,HEV-C1 ,Italy ,Medicine - Abstract
Hepatitis E virus belonging to the Rocahepevirus ratti species, genotype HEV-C1, has been extensively reported in rats in Europe, Asia and North America. Recently, human cases of hepatitis associated with HEV-C1 infection have been reported, but the zoonotic nature of rat-HEV remains controversial. The transmission route of rat-HEV is unidentified and requires further investigation. The HEV strains of the Paslahepevirus balayani species, belonging to the same Hepeviridae family, and including the zoonotic genotype HEV-3 usually found in pigs, have also sporadically been identified in rats. We sampled 115 rats (liver, lung, feces) between 2020 and 2023 in Northeast Italy and the HEV detection was carried out by using Reverse Transcription PCR. HEV RNA was detected in 3/115 (2.6%) rats who tested positive for HEV-C1 strains in paired lung, intestinal contents and liver samples. Overall, none tested positive for the Paslahepevirus balayani strains. In conclusion, our results confirm the presence of HEV-rat in Italy with a prevalence similar to previous studies but show that there is a wide heterogeneity of strains in circulation. The detection of HEV-C1 genotype of Rocahepevirus ratti species in some human cases of acute hepatitis suggests that HEV-C1 may be an underestimated source of human infections. This finding, with the geographically widespread detection of HEV-C1 in rats, raises questions about the role of rats as hosts for both HEV-C1 and HEV-3 and the possibility of zoonotic transmission.
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- 2024
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13. Evaluation and deployment of isotype-specific salivary antibody assays for detecting previous SARS-CoV-2 infection in children and adults
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Thomas, Amy C., Oliver, Elizabeth, Baum, Holly E., Gupta, Kapil, Shelley, Kathryn L., Long, Anna E., Jones, Hayley E., Smith, Joyce, Hitchings, Benjamin, di Bartolo, Natalie, Vasileiou, Kate, Rabi, Fruzsina, Alamir, Hanin, Eghleilib, Malak, Francis, Ore, Oliver, Jennifer, Morales-Aza, Begonia, Obst, Ulrike, Shattock, Debbie, Barr, Rachael, Collingwood, Lucy, Duale, Kaltun, Grace, Niall, Livera, Guillaume Gonnage, Bishop, Lindsay, Downing, Harriet, Rodrigues, Fernanda, Timpson, Nicholas, Relton, Caroline L., Toye, Ashley, Woolfson, Derek N., Berger, Imre, Goenka, Anu, Davidson, Andrew D., Gillespie, Kathleen M., Williams, Alistair J. K., Bailey, Mick, Brooks-Pollock, Ellen, Finn, Adam, and Halliday, Alice
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- 2023
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14. Biosecurity measures to control hepatitis E virus on European pig farms
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Tamino Dubbert, Marina Meester, Richard Piers Smith, Tijs J. Tobias, Ilaria Di Bartolo, Reimar Johne, Enrico Pavoni, Gergana Krumova-Valcheva, Elena Lucia Sassu, Christopher Prigge, Giuseppe Aprea, Hannah May, Nadine Althof, Giovanni Ianiro, Jacek Żmudzki, Albena Dimitrova, Giovanni Loris Alborali, Daniela D'Angelantonio, Silvia Scattolini, Noemi Battistelli, and Elke Burow
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hepatitis-E-virus ,HEV ,biosecurity measures ,pig production ,BIOPIGEE ,One Health ,Veterinary medicine ,SF600-1100 - Abstract
Hepatitis E virus (HEV) genotype 3 is a prevalent zoonotic pathogen in European pig farms, posing a significant public health risk primarily through the foodborne route. The study aimed to identify effective biosecurity measures for controlling HEV transmission on pig farms, addressing a critical gap in current knowledge. Utilizing a cross-sectional design, fecal samples from gilts, dry sows, and fatteners were collected on 231 pig farms of all farm types across nine European countries. Real-time RT-PCR was employed to test these samples for HEV. Simultaneously, a comprehensive biosecurity questionnaire captured data on various potential measures to control HEV. The dependent variable was HEV risk, categorized as lower or higher based on the percentage of positive pooled fecal samples on each farm (25% cut-off). The data were analyzed using generalized linear models (one for finisher samples and one for all samples) with a logit link function with country and farm type as a priori fixed factors. The results of the final multivariable models identified key biosecurity measures associated with lower HEV risk, which were the use of a hygienogram in the breeding (OR: 0.06, p = 0.001) and/or fattening area after cleaning (OR: 0.21, p = 0.019), the presence of a quarantine area (OR: 0.29, p = 0.025), testing and/or treating purchased feed against Salmonella (OR: 0.35, p = 0.021), the presence of other livestock species on the farm, and having five or fewer persons in charge of the pigs. Contrary to expectations, some biosecurity measures were associated with higher HEV risk, e.g., downtime of 3 days or longer after cleaning in the fattening area (OR: 3.49, p = 0.005) or mandatory handwashing for farm personnel when changing barn sections (OR: 3.4, p = 0.026). This novel study unveils critical insights into biosecurity measures effective in controlling HEV on European pig farms. The identification of both protective and risk-associated measures contributes to improving strategies for managing HEV and underscores the complexity of biosecurity in pig farming.
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- 2024
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15. Sex Related Differences and Factors Associated With Peri-Procedural and One Year Mortality in Chronic Limb Threatening Ischaemia Patients
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Eugenio Martelli, Matilde Zamboni, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Sangiorgi, Mariangela Valentina Puci, Allegra Rosa Martelli, Teresa Messina, Paolo Frigatti, Maria Pia Borrelli, Carlo Ruotolo, Ilaria Ficarelli, Paolo Rubino, Francesco Pezzo, Luciano Carbonari, Andrea Angelini, Edoardo Galeazzi, Luca Calia di Pinto, Franco Michelino Fiore, Armando Palmieri, Giorgio Ventoruzzo, Giulia Mazzitelli, Franco Ragni, Antonio Bozzani, Enzo Forliti, Claudio Castagno, Pietro Volpe, Mafalda Massaro, Diego Moniaci, Elisa Pagliasso, Tania Peretti, Mauro Ferrari, Nicola Troisi, Piero Modugno, Maurizio Maiorano, Umberto Marcello Bracale, Marco Panagrosso, Mario Monaco, Giovanni Giordano, Giuseppe Natalicchio, Antonella Biello, Giovanni Maria Celoria, Alessio Amico, Mauro Di Bartolo, Massimiliano Martelli, Roberta Munaò, Davide Razzano, Giovanni Colacchio, Francesco Bussetti, Gaetano Lanza, Antonio Cardini, Bartolomeo Di Benedetto, Mario De Laurentis, Maurizio Taurino, Pasqualino Sirignano, Pierluigi Cappiello, Andrea Esposito, Santi Trimarchi, Silvia Romagnoli, Andrea Padricelli, Giorgio Giudice, Adolfo Crinisio, Giovanni Di Nardo, Giuseppe Battaglia, Rosario Tringale, Salvatore De Vivo, Rita Compagna, Valerio Stefano Tolva, Ilenia D'Alessio, Ruggiero Curci, Simona Giovannetti, Giuseppe D'Arrigo, Giusi Basile, Dalmazio Frigerio, Gianfranco Veraldi, Luca Mezzetto, Arnaldo Ippoliti, Fabio Massimo Oddi, and Alberto Maria Settembrini
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Diseases of the circulatory (Cardiovascular) system ,RC666-701 ,Surgery ,RD1-811 - Published
- 2024
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16. Selection of a novel cell-internalizing RNA aptamer specific for CD22 antigen in B cell acute lymphoblastic leukemia
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Dario Ruiz-Ciancio, Li-Hsien Lin, Suresh Veeramani, Maya N. Barros, Diego Sanchez, Ary Lautaro Di Bartolo, Diego Masone, Paloma H. Giangrande, María Belén Mestre, and William H. Thiel
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MT: Delivery Strategies ,aptamer ,cell internalization SELEX ,CD22 protein ,B cell acute lymphoblastic leukemia ,molecular docking ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Despite improvements in B cell acute lymphoblastic leukemia (B-ALL) treatment, a significant number of patients experience relapse of the disease, resulting in poor prognosis and high mortality. One of the drawbacks of current B-ALL treatments is the high toxicity associated with the non-specificity of chemotherapeutic drugs. Targeted therapy is an appealing strategy to treat B-ALL to mitigate these toxic off-target effects. One such target is the B cell surface protein CD22. The restricted expression of CD22 on the B-cell lineage and its ligand-induced internalizing properties make it an attractive target in cases of B cell malignancies. To target B-ALL and the CD22 protein, we performed cell internalization SELEX (Systematic Evolution of Ligands by EXponential enrichment) followed by molecular docking to identify internalizing aptamers specific for B-ALL cells that bind the CD22 cell-surface receptor. We identified two RNA aptamers, B-ALL1 and B-ALL2, that target human malignant B cells, with B-ALL1 the first documented RNA aptamer interacting with the CD22 antigen. These B-ALL-specific aptamers represent an important first step toward developing novel targeted therapies for B cell malignancy treatments.
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- 2023
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17. Exploring the Potential of Muridae as Sentinels for Human and Zoonotic Viruses
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Ilaria Di Bartolo, Luca De Sabato, Giovanni Ianiro, Gabriele Vaccari, Filippo Maria Dini, Fabio Ostanello, and Marina Monini
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Muridae ,rats ,mice ,viral zoonoses ,rodents ,reservoir ,Microbiology ,QR1-502 - Abstract
In recent years, the transmission of viruses from wildlife to humans has raised significant public health concerns, exemplified by the COVID-19 pandemic caused by the betacoronavirus SARS-CoV-2. Human activities play a substantial role in increasing the risk of zoonotic virus transmission from wildlife to humans. Rats and mice are prevalent in urban environments and may act as reservoirs for various pathogens. This study aimed to evaluate the presence of zoonotic viruses in wild rats and mice in both urban and rural areas, focusing on well-known zoonotic viruses such as betacoronavirus, hantavirus, arenavirus, kobuvirus, and monkeypox virus, along with other viruses occasionally detected in rats and mice, including rotavirus, norovirus, and astrovirus, which are known to infect humans at a high rate. A total of 128 animals were captured, including 70 brown rats (Rattus norvegicus), 45 black rats (Rattus rattus), and 13 house mice (Mus musculus), and feces, lung, and liver were collected. Among brown rats, one fecal sample tested positive for astrovirus RNA. Nucleotide sequencing revealed high sequence similarity to both human and rat astrovirus, suggesting co-presence of these viruses in the feces. Murine kobuvirus (MuKV) was detected in fecal samples from both black (n = 7) and brown (n = 6) rats, primarily from urban areas, as confirmed by sequence analysis. These findings highlight the importance of surveillance and research to understand and mitigate the risks associated with the potential transmission of pathogens by rodents.
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- 2024
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18. Identification of Aichivirus in a Pet Rat (Rattus norvegicus) in Italy
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Flora Alfano, Maria Gabriella Lucibelli, Francesco Serra, Martina Levante, Simona Rea, Amalia Gallo, Federica Petrucci, Alessia Pucciarelli, Gerardo Picazio, Marina Monini, Ilaria Di Bartolo, Dario d’Ovidio, Mario Santoro, Esterina De Carlo, Giovanna Fusco, and Maria Grazia Amoroso
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aichivirus ,Rattus norvegicus ,exotic pets ,kobuvirus ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
We investigated the occurrence of eight potential zoonotic viruses in 91 exotic companion mammals from pet shops in southern Italy via real-time PCR and end-point PCR. The animals were screened for aichivirus, sapovirus, astrovirus, hepatitis A, noroviruses (GI and GII), rotavirus, circovirus, and SARS-CoV-2. Among the nine species of exotic pets studied, only one rat tested positive for aichivirus. The high sequence similarity to a murine kobuvirus-1 strain previously identified in China suggests that the virus may have been introduced into Italy through the importation of animals from Asia. Since exotic companion mammals live in close contact with humans, continuous sanitary monitoring is crucial to prevent the spread of new pathogens among domestic animals and humans. Further investigations on detecting and typing zoonotic viruses are needed to identify emerging and re-emerging viruses to safeguard public health.
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- 2024
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19. Selective Pressure and Evolution of SARS-CoV-2 Lineages BF.7 and BQ.1.1 Circulating in Italy from July to December 2022
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Alessandra Lo Presti, Luigina Ambrosio, Angela Di Martino, Arnold Knijn, Luca De Sabato, Gabriele Vaccari, Ilaria Di Bartolo, Stefano Morabito, Anna Teresa Palamara, Paola Stefanelli, and on behalf of the Italian Genomic Laboratory Network
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genetic diversity ,mutation ,phylogenetic analysis ,SARS-CoV-2 ,selective pressure ,Biology (General) ,QH301-705.5 - Abstract
In this work, we studied the selective pressure and evolutionary analysis on the SARS-CoV-2 BF.7 and BQ.1.1 lineages circulating in Italy from July to December 2022. Two different datasets were constructed: the first comprised 694 SARS-CoV-2 BF.7 lineage sequences and the second comprised 734 BQ.1.1 sequences, available in the Italian COVID-19 Genomic (I-Co-Gen) platform and GISAID (last access date 15 December 2022). Alignments were performed with MAFFT v.7 under the Galaxy platform. The HYPHY software was used to study the selective pressure. Four positively selected sites (two in nsp3 and two in the spike) were identified in the BF.7 dataset, and two (one in ORF8 and one in the spike gene) were identified in the BQ.1.1 dataset. Mutation analysis revealed that R408S and N440K are very common in the spike of the BF.7 genomes, as well as L452R among BQ.1.1. N1329D and Q180H in nsp3 were found, respectively, at low and rare frequencies in BF.7, while I121L and I121T were found to be rare in ORF8 for BQ.1.1. The positively selected sites may have been driven by the selection for increased viral fitness, under circumstances of defined selective pressure, as well by host genetic factors.
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- 2024
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20. A Review of Slaughter Practices and Their Effectiveness to Control Microbial – esp. Salmonella spp. – Contamination of Pig Carcasses
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Arvo Viltrop, Tarmo Niine, Tijs Tobias, Elena Lucia Sassu, Ilaria Di Bartolo, Enrico Pavoni, Giovanni Loris Alborali, Elke Burow, and Richard Piers Smith
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Abattoir ,Biosecurity ,Slaughter hygiene ,Swine ,Pork ,Hepatitis E virus ,Food processing and manufacture ,TP368-456 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
The BIOPIGEE project (part of the One Health European Joint Programme under Horizon 2020) aimed to identify relevant measures to effectively control Salmonella, and another zoonotic pathogen, hepatitis E virus (HEV) within the pig meat food chain. The aim of this study was to identify biosecurity measures or management practices that are relevant for limiting Salmonella and/or HEV occurrence and spread within pig slaughterhouses. This was with the final goal of compiling a list of biosecurity measures for different processes and operations along the slaughter line with evidence of their effectiveness. To achieve this, a literature review was conducted on studies estimating the effectiveness of measures applied in slaughterhouses to reduce the microbial contamination of pig carcasses. Results of this literature search are discussed and presented in summary tables that could be used as a source of information for the pig slaughter industry to further develop their guidelines on hygienic slaughter.
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- 2023
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21. Precision farming technologies for crop protection: A meta-analysis
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Evangelos Anastasiou, Spyros Fountas, Matina Voulgaraki, Vasilios Psiroukis, Michael Koutsiaras, Olga Kriezi, Erato Lazarou, Anna Vatsanidou, Longsheng Fu, Fabiola Di Bartolo, Jesus Barreiro-Hurle, and Manuel Gómez-Barbero
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Pesticide use ,Precision farming ,Crop protection ,Robots ,Sensors ,Platforms ,Agriculture (General) ,S1-972 ,Agricultural industries ,HD9000-9495 - Abstract
Precision farming technologies (PFTs) can have a significant impact on reducing the dependency on plant protection products (PPP) for crop protection. PFTs for crop protection comprises an extensive suite of digital solutions that can be used to predict, detect, and control pests. In this paper, we report the results of a systematic literature meta-analysis to map PFTs in relation to crop protection and identify trends and gaps in the use of these technologies in order for them to be easily adopted by farmers. In total, 239 different research articles were assessed in terms of sensor, platform, crop, pest, pest management stage, and impact type. The majority of research articles focused on arable crops and on weed management. The identified PFTs can achieve up to 97 % savings in herbicides, reduce the area that needs insecticide application by up to 70 %, and reduce weed densities by 89 %. In the future, high resolutions images and proximal sensing with heterogeneous robotic systems is expected to make pest detection and control more efficient.
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- 2023
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22. Precision farming technologies on crop protection: A stakeholders survey
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Evangelos Anastasiou, Spyros Fountas, Michael Koutsiaras, Matina Voulgaraki, Anna Vatsanidou, Jesus Barreiro-Hurle, Fabiola Di Bartolo, and Manuel Gómez-Barbero
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Pesticide use ,Small and medium-sized farmers ,Precision farming ,Crop protection ,Adoption ,Policies ,Agriculture (General) ,S1-972 ,Agricultural industries ,HD9000-9495 - Abstract
Although precision farming technologies (PFTs) have the ability to reduce the use of pesticides, delivering on this potential relies on the adoption of PFTs by the farming community. With small and medium-sized farmers representing more than 95% of the total number of farmers while cultivating less than 30% of the total cultivated area globally, and presenting a low adoption rate of PFTs, their adoption of PFTs becomes key to understand the global potential of these technologies. In this paper we report an expert knowledge elicitation of the main aspects of PFTs adoption by small and medium-sized farmers covering the perceived usefulness for their farms, the main barriers for adoption and the role of agricultural institutions and policies in overcoming these. Data were obtained via an online survey which was answered by 175 agricultural experts from around the globe. From the analysis of the responses, we can conclude that the usefulness of PFTs are crop and technology specific. Nearly all respondents considered the lack of technical support to be the most important limiting factor for the adoption of PFTs and identified farmers who are already adopters of PFTs and farmer cooperatives as the most promising agents for disseminating this support and increasing adoption by other farmers. Provision of incentives to lower investment costs together with advisory services were the most important policy interventions identified by respondents to foster the adoption of PFTs by small and medium-sized farmers. With regards to the way PFTs would be implemented in practice, most respondents believed that the PFTs will be purchased as a service from private companies by small and medium-sized farmers. Finally, future research is needed to study the impact of policies on the adoption of PFTs for crop protection in order to identify optimized policy pathways to enhance their adoption by small and medium-sized farmers.
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- 2023
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23. Evaluation and deployment of isotype-specific salivary antibody assays for detecting previous SARS-CoV-2 infection in children and adults
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Amy C. Thomas, Elizabeth Oliver, Holly E. Baum, Kapil Gupta, Kathryn L. Shelley, Anna E. Long, Hayley E. Jones, Joyce Smith, Benjamin Hitchings, Natalie di Bartolo, Kate Vasileiou, Fruzsina Rabi, Hanin Alamir, Malak Eghleilib, Ore Francis, Jennifer Oliver, Begonia Morales-Aza, Ulrike Obst, Debbie Shattock, Rachael Barr, Lucy Collingwood, Kaltun Duale, Niall Grace, Guillaume Gonnage Livera, Lindsay Bishop, Harriet Downing, Fernanda Rodrigues, Nicholas Timpson, Caroline L. Relton, Ashley Toye, Derek N. Woolfson, Imre Berger, Anu Goenka, Andrew D. Davidson, Kathleen M. Gillespie, Alistair J. K. Williams, Mick Bailey, Ellen Brooks-Pollock, Adam Finn, Alice Halliday, and the CoMMinS Study Team
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Medicine - Abstract
Thomas et al. test 6 ELISAs detecting IgA and IgG antibodies to whole SARS-CoV-2 spike protein, to its receptor binding domain region and to nucleocapsid protein in saliva. Across 20 household outbreaks, antibody responses are heterogeneous, but a reliable indicator of recent infection.
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- 2023
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24. First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria
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Amir Agabou, Mohamed Hocine Benaissa, Ilyes Bouasla, Luca De Sabato, Sana Hireche, Giovanni Ianiro, Marina Monini, and Ilaria Di Bartolo
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hepatitis E virus ,ELISA ,seroprevalence ,risk factors ,dromedary camels ,Algeria ,Veterinary medicine ,SF600-1100 - Abstract
Hepatitis E is an acute self-limited or fulminant infection in humans, caused by the hepatitis E virus (HEV). This member of the Hepeviridae family has been identified in a wide range of domestic and wild animals all over the world, with a possible transmission to humans through fecal oral route, direct contact and ingestion of contaminated meat products, making it one of the global zoonotic and public health major concerns. Since there is no monitoring program and a lack of data on HEV in animals in Algeria, the current preliminary survey has been undertaken to elucidate the exposure to the virus in camels at abattoirs of six southern provinces of Algeria. Two-hundred and eight sera/plasma were collected and analyzed (by double antigen sandwich ELISA) for the presence of total anti-HEV antibodies, among which 35.1% were positive, but no HEV RNA could be isolated from them (by two pan-HEV nested RT-PCR and broad range real-time reverse transcription RT-PCR). The univariate analysis showed significant associations (p
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- 2023
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25. Erinaceus coronavirus persistence in hedgehogs (Erinaceus europaeus) in a non-invasive, in vivo, experimental setting
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Luca De Sabato, Giovanni Ianiro, Francesca Manzia, Marina Monini, Barbara Chiappini, Ilaria Di Bartolo, and Gabriele Vaccari
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Merbecovirus ,Erinaceus europaeus ,Erinaceus coronavirus (EriCoV) ,Coronavirus ,European hedgehogs ,Veterinary medicine ,SF600-1100 - Abstract
In the last 20 years, new zoonotic CoV strains have emerged (SARS-CoV, MERS-CoV, and SARS-CoV-2), and new species have also been reported in animals. In Europe, the Erinaceus coronavirus (EriCoV) was recently described in Erinaceus europaeus. However, information on the prevalence and duration of viral shedding is unknown. In this study, feces samples were collected from 102 European hedgehogs hosted in the Center for the Recovery of Wild Fauna in Rome and analyzed for the presence of EriCoV RNA by Reverse Transcription-PCR. In total, 45 animals (44.1%) resulted positive for EriCoV at the first sampling and 63 (61.7%) animals were positive at the follow-up, which was performed from the 3rd to the 86th day. The duration of fecal virus shedding showed a mean duration of 22.8 days and lasted up to 62 days. Eighteen hedgehogs showed intermittent viral shedding. Phylogenetic analysis showed a correlation with EriCoV strains reported in Germany, the United Kingdom, and northern Italy. None of the EriCoV sequences showed the CD200 ortholog insertion, previously observed in strains isolated in animals from northern Italy. Interestingly, all but one animal revealed the presence in their feces of the same EriCoV sequences, analyzing the short genomic region at 3' spike gene and 5' ORF3a 500bp fragment (100% nt.id.) in both first and follow-up samples. This result suggests that animals were infected with the same strain during their stay at the center. Our results confirm that EriCoV can persist in hedgehogs for a long period, underlining that hedgehogs are an important commensal reservoir for Merbecovirus. A long duration of viral shedding increases the likelihood that the virus will spread in the environment.
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- 2023
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26. The legacy effect of hyperglycemia and early use of SGLT-2 inhibitors: a cohort study with newly-diagnosed people with type 2 diabetesResearch in context
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Antonio Ceriello, Giuseppe Lucisano, Francesco Prattichizzo, Rosalba La Grotta, Chiara Frigé, Salvatore De Cosmo, Paolo Di Bartolo, Graziano Di Cianni, Paola Fioretto, Carlo Bruno Giorda, Roberto Pontremoli, Giuseppina Russo, Francesca Viazzi, and Antonio Nicolucci
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AMD Annals initiative ,Type 2 diabetes ,Metabolic memory ,Legacy effect ,Cardiovascular diseases ,Sodium-glucose cotransporter 2 inhibitors ,Public aspects of medicine ,RA1-1270 - Abstract
Summary: Background: A delay in reaching HbA1c targets in patients with newly-diagnosed type 2 diabetes (T2D) is associated with an increased long-term risk of developing cardiovascular diseases (CVD), a phenomenon referred to as legacy effect. Whether an early introduction of glucose-lowering drugs with proven benefit on CVD can attenuate this phenomenon is unknown. Methods: Using data derived from a large Italian clinical registry, i.e. the AMD Annals, we identified 251,339 subjects with newly-diagnosed T2D and without CVD at baseline. Through Cox regressions adjusted for multiple risk factors, we examined the association between having a mean HbA1c between 7.1 and 8% or >8%, compared with ≤7%, for various periods of early exposure (0–1, 0–2, 0–3 years) and the development of later (mean subsequent follow-up 4.6 ± 2.9 years) CVD, evaluated as a composite of myocardial infarction, stroke, coronary or peripheral revascularization, and coronary or peripheral bypass. We performed this analysis in the overall cohort and then splitting the population in two groups of patients: those that introduced sodium-glucose transport protein 2 inhibitors (SGLT-2i) during the exposure phase and those not treated with these drugs. Findings: Considering the whole cohort, subjects with both a mean HbA1c between 7.1 and 8% and >8%, compared with patients attaining a mean HbA1c ≤ 7%, showed an increased risk of developing the outcome in all the three early exposure periods assessed, with the highest risk observed in patients with mean HbA1c > 8% in the 3 years exposure period (hazard ratio [HR]1.33; 95% confidence interval [CI] 1.063–1.365). The introduction of SGLT-2i during the exposure periods of 0–1 and 0–2 years eliminated the association between poor glycemic control and the outcome (p for interaction 0.006 and 0.003, respectively, vs. patients with the same degree of glycemic control but not treated with these drugs). Interpretation: Among patients with newly diagnosed T2D and free of CVD at baseline, a poor glycemic control in the first three years after diagnosis is associated with an increased subsequent risk of CVD. This association is no longer evident when SGLT-2i are introduced in the first two years, suggesting that these drugs attenuate the phenomenon of legacy effect. An early treatment with these drugs might thus promote a long-lasting benefit in patients not attaining proper glycemic control after T2D diagnosis. Funding: This work was supported, in part, by the Italian Ministry of Health (Ricerca Corrente) to IRCCS MultiMedica.
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- 2023
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27. T cell migration and effector function differences in familial adenomatous polyposis patients with APC gene mutations
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Céline Cuche, Marta Mastrogiovanni, Marie Juzans, Hélène Laude, Marie-Noëlle Ungeheuer, Daniel Krentzel, Maria Isabella Gariboldi, Daniel Scott-Algara, Marianne Madec, Sophie Goyard, Camille Floch, Gaëlle Chauveau-Le Friec, Pierre Lafaye, Charlotte Renaudat, Muriel Le Bidan, Christine Micallef, Sandrine Schmutz, Sébastien Mella, Sophie Novault, Milena Hasan, Darragh Duffy, Vincenzo Di Bartolo, and Andrés Alcover
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Adenomatous polyposis coli ,APC ,familial adenomatous polyposis ,T cell activation ,T cell migration ,cytokines ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Familial adenomatous polyposis (FAP) is an inherited disease characterized by the development of large number of colorectal adenomas with high risk of evolving into colorectal tumors. Mutations of the Adenomatous polyposis coli (APC) gene is often at the origin of this disease, as well as of a high percentage of spontaneous colorectal tumors. APC is therefore considered a tumor suppressor gene. While the role of APC in intestinal epithelium homeostasis is well characterized, its importance in immune responses remains ill defined. Our recent work indicates that the APC protein is involved in various phases of both CD4 and CD8 T cells responses. This prompted us to investigate an array of immune cell features in FAP subjects carrying APC mutations. A group of 12 FAP subjects and age and sex-matched healthy controls were studied. We characterized the immune cell repertoire in peripheral blood and the capacity of immune cells to respond ex vivo to different stimuli either in whole blood or in purified T cells. A variety of experimental approaches were used, including, pultiparamater flow cytometry, NanosString gene expression profiling, Multiplex and regular ELISA, confocal microscopy and computer-based image analyis methods. We found that the percentage of several T and natural killer (NK) cell populations, the expression of several genes induced upon innate or adaptive immune stimulation and the production of several cytokines and chemokines was different. Moreover, the capacity of T cells to migrate in response to chemokine was consistently altered. Finally, immunological synapses between FAP cytotoxic T cells and tumor target cells were more poorly structured. Our findings of this pilot study suggest that mild but multiple immune cell dysfunctions, together with intestinal epithelial dysplasia in FAP subjects, may facilitate the long-term polyposis and colorectal tumor development. Although at an initial discovery phase due to the limited sample size of this rare disease cohort, our findings open new perspectives to consider immune cell abnormalities into polyposis pathology.
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- 2023
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28. Adapting an Empirical Clinic-Based Organizational Skills Training Program for Use in Schools: Iterative Development and Pilot Assessment
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Di Bartolo, Christina A., Abikoff, Howard, McDonald, Margaret, and Gallagher, Richard
- Abstract
Effective organizational skills (e.g., time management, materials management, and task planning) are associated with academic success, yet some children struggle to gain these skills without intervention. While evidence-based organizational skills interventions for pediatric clinical populations exist, none target organizationally impaired general populations in a primary school setting. In Organizational Skills Training-School (OST-S), school professionals teach small groups of 4th-5th grade organizationally impaired elementary school students skills associated with tracking assignments, managing materials, time management, and planning. This open pilot study of 33 students examined: a. the feasibility of adapting and implementing an established clinic-based, individualized treatment (OST-C) to a group format in a school setting, b. the program's acceptability among children, parents, and school partners, and c. whether participating children improved in the same functional areas that OST-C positively influenced. Results indicated that we could adapt the intervention, school partners could implement it, and participants could accept it. Pre-post improvements in organizational skills (as rated by parents, teachers, and children), homework problems, and academic performance demonstrated the potential effectiveness of OST-S, suggesting the need for further efficacy work.
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- 2021
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29. α-Synuclein is required for sperm exocytosis at a post-fusion stage
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Micaela Vanina Buzzatto, María Victoria Berberián, Ary Lautaro Di Bartolo, Diego Masone, and Claudia Nora Tomes
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acrosome exocytosis ,cell permeabilization ,exocytosis ,fusion pore ,sperm ,α-synuclein ,Biology (General) ,QH301-705.5 - Abstract
The sperm acrosome is a large dense-core granule whose contents are secreted by regulated exocytosis at fertilization through the opening of numerous fusion pores between the acrosomal and plasma membranes. In other cells, the nascent pore generated when the membrane surrounding a secretory vesicle fuses with the plasma membrane may have different fates. In sperm, pore dilation leads to the vesiculation and release of these membranes, together with the granule contents. α-Synuclein is a small cytosolic protein claimed to exhibit different roles in exocytic pathways in neurons and neuroendocrine cells. Here, we scrutinized its function in human sperm. Western blot revealed the presence of α-synuclein and indirect immunofluorescence its localization to the acrosomal domain of human sperm. Despite its small size, the protein was retained following permeabilization of the plasma membrane with streptolysin O. α-Synuclein was required for acrosomal release, as demonstrated by the inability of an inducer to elicit exocytosis when permeabilized human sperm were loaded with inhibitory antibodies to human α-synuclein. The antibodies halted calcium-induced secretion when introduced after the acrosome docked to the cell membrane. Two functional assays, fluorescence and transmission electron microscopies revealed that the stabilization of open fusion pores was responsible for the secretion blockage. Interestingly, synaptobrevin was insensitive to neurotoxin cleavage at this point, an indication of its engagement in cis SNARE complexes. The very existence of such complexes during AE reflects a new paradigm. Recombinant α-synuclein rescued the inhibitory effects of the anti-α-synuclein antibodies and of a chimeric Rab3A-22A protein that also inhibits AE after fusion pore opening. We applied restrained molecular dynamics simulations to compare the energy cost of expanding a nascent fusion pore between two model membranes and found it higher in the absence than in the presence of α-synuclein. Hence, our results suggest that α-synuclein is essential for expanding fusion pores.
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- 2023
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30. Cross-sectional study of hepatitis E virus (HEV) circulation in Italian pig farms
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Giovanni Ianiro, Enrico Pavoni, Giuseppe Aprea, Romina Romantini, Giovanni Loris Alborali, Daniela D'Angelantonio, Giuliano Garofolo, Silvia Scattolini, Luca De Sabato, Chiara Francesca Magistrali, Elke Burow, Fabio Ostanello, Richard Piers Smith, and Ilaria Di Bartolo
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hepatitis E virus ,HEV ,zoonoses ,pig ,feces ,farm ,Veterinary medicine ,SF600-1100 - Abstract
Foodborne transmission is considered the main way of spreading zoonotic hepatitis E virus (HEV) infection in Europe. In recent years, the human cases of hepatitis E in subjects without history of travel in endemic areas have raised, suggesting that domestic HEV transmission is increasing. Pork products with or without liver, are often indicated as the source of many human foodborne HEV cases as well as small outbreaks. Pigs are recognized as the main reservoir of the zoonotic HEV-3 genotype, the most frequently detected in human cases in the EU. In the absence of a harmonized surveillance of HEV circulation, data on prevalence are heterogeneous but confirm a widespread circulation of HEV-3 in pig herds across EU. HEV-3 can pass through the food chain from farm to fork when infected animals are slaughtered. In Italy, several studies reported the circulation of HEV-3 in pig farms, but results are heterogeneous due to different methodologies applied. In the present study, we performed a survey over 51 pig herds belonging to three main types of farms: breeding, fattening and farrow-to-finish. HEV-RNA was analyzed by broad range Real-time RT-PCR on 20 samples for each farm, obtained by pooling together feces from 10 individuals. Overall, HEV RNA was confirmed on 150 fecal pooled samples out of 1,032 (14.5%). At least one positive pooled sample was detected from 18 farms out of 51 tested (35.3%). By lowering the number of infected pigs at primary production, the risk of HEV-3 entering into the food chain can be reduced. Hence, information on HEV circulation in herds is highly relevant for choosing preventive measures and deserves development of a monitoring program and further investigations.
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- 2023
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31. Tracking the Selective Pressure Profile and Gene Flow of SARS-CoV-2 Delta Variant in Italy from April to October 2021 and Frequencies of Key Mutations from Three Representative Italian Regions
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Alessandra Lo Presti, Angela Di Martino, Luigina Ambrosio, Luca De Sabato, Arnold Knijn, Gabriele Vaccari, Ilaria Di Bartolo, Stefano Morabito, Calogero Terregino, Alice Fusaro, Isabella Monne, Edoardo Giussani, Fabio Tramuto, Carmelo Massimo Maida, Walter Mazzucco, Claudio Costantino, Martina Rueca, Emanuela Giombini, Cesare Ernesto Maria Gruber, Maria Rosaria Capobianchi, Anna Teresa Palamara, Paola Stefanelli, and on behalf of the Italian Genomic Laboratory Network
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gene flows ,mutations ,SARS-CoV-2 Delta variant ,selective pressure ,Biology (General) ,QH301-705.5 - Abstract
The SARS-CoV-2 Delta variant of concern (VOC) was often associated with serious clinical course of the COVID-19 disease. Herein, we investigated the selective pressure, gene flow and evaluation on the frequencies of mutations causing amino acid substitutions in the Delta variant in three Italian regions. A total of 1500 SARS-CoV-2 Delta genomes, collected in Italy from April to October 2021 were investigated, including a subset of 596 from three Italian regions. The selective pressure and the frequency of amino acid substitutions and the prediction of their possible impact on the stability of the proteins were investigated. Delta variant dataset, in this study, identified 68 sites under positive selection: 16 in the spike (23.5%), 11 in nsp2 (16.2%) and 10 in nsp12 (14.7%) genes. Three of the positive sites in the spike were located in the receptor-binding domain (RBD). In Delta genomes from the three regions, 6 changes were identified as very common (>83.7%), 4 as common (>64.0%), 21 at low frequency (2.1%–25.0%) and 29 rare (≤2.0%). The detection of positive selection on key mutations may represent a model to identify recurrent signature mutations of the virus.
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- 2023
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32. Targeting Implicit Bias in Medicine: Lessons from Art and Archaeology
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Zeidan, Amy, Tiballi, Anne, Woodward, Melanie, and Di Bartolo, Isha Marina
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Implicit Bias - Abstract
Implicit bias training is not currently a required component of residency education, yet implicit bias in medicine exists and may influence care provided to patients. We propose an innovative exercise that allows trainees to explore implicit bias outside of the clinical environment, in an interdisciplinary manner with museum anthropologists and archaeologists. The curriculum was designed with leaders at the Penn Museum and focuses on differentiating between objective and subjective assessments of historical objects. The first part of the exercise consists of a pre-brief, to introduce trainees to bias through the lens of an anthropologist/archaeologist. The second part guides trainees through“deep description,” where they explore objective and subjective findings of three different objects. The exercise concludes with a debrief and application of concepts learned to everyday clinical practice. This innovation was successful at introducing trainees to implicit bias in a nontraditional environment, and participants reported an improved understanding of implicit bias. Residency programs could consider partnering with local museums to implement a similar exercise as acomponent of conference curriculum.
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- 2019
33. Emergency department accesses for diabetes-related complications during COVID-19 pandemic in people with type 2 diabetes and depression
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Messina, Rossella, Iommi, Marica, Rucci, Paola, Reno, Chiara, Mazzotti, Arianna, Caletti, Maria Turchese, Altini, Mattia, Bravi, Francesca, Fantini, Maria Pia, and Di Bartolo, Paolo
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- 2022
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34. Is it time to consider depression as a major complication of type 2 diabetes? Evidence from a large population-based cohort study
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Messina, Rossella, Iommi, Marica, Rucci, Paola, Reno, Chiara, Fantini, Maria Pia, Lunghi, Carlotta, Altini, Mattia, Bravi, Francesca, Rosa, Simona, Nicolucci, Antonio, and Di Bartolo, Paolo
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- 2022
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35. A monoidal representation for linearized gravity
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Contreras, Ernesto, Di Bartolo, Cayetano, and Leal, Lorenzo
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High Energy Physics - Theory - Abstract
We propose an alternative representation for linear quantum gravity. It is based on the use of a structure that bears some resemblance to the Abelian loop representation used in electromagnetism but with the difference that space of extended object on which waves functions take values have a structure of commutative monoid instead of Abelian group. The generator of duality of the theory is realized in this representation and a geometrical interpretation is discussed.
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- 2016
36. Hepatitis E Virus (HEV) in Heavy Pigs in Slaughterhouses of Northern Italy: Investigation of Seroprevalence, Viraemia, and Faecal Shedding
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Marina Monini, Ilaria Di Bartolo, Luca De Sabato, Giovanni Ianiro, Francesca Agostinelli, and Fabio Ostanello
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HEV ,zoonoses ,pigs ,slaughterhouse ,viraemia ,IgM ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Hepatitis E virus (HEV) is considered an emerging threat in Europe, owing to the increased number of human cases and the widespread presence of the virus in pigs at farms. Most cases in industrialized countries are caused by the zoonotic HEV-3 genotype. The main transmission route of HEV-3 in Europe is foodborne, through consumption of raw or undercooked liver pork and wild boar meat. Pigs become susceptible to HEV infection after the loss of maternal immunity, and the majority of adult pigs test positive for IgG anti-HEV antibodies. Nonetheless, HEV-infected pigs in terms of liver, faeces, and rarely blood are identified at slaughterhouses. The present study aimed to investigate the prevalence of HEV-positive batches of Italian heavy pigs at slaughterhouses, assessing the presence of animals still shedding HEV upon their arrival at the slaughterhouse by sampling faeces collected from the floor of the trucks used for their transport. The occurrence of viraemic animals and the seroprevalence of anti-HEV antibodies were also assessed. The results obtained indicated the presence of anti-HEV IgM (1.9%), and a high seroprevalence of anti-HEV total antibodies (IgG, IgM, IgA; 89.2%, n = 260). HEV RNA was not detected in either plasma or faecal samples. Nevertheless, seropositive animals were identified in all eight batches investigated, confirming the widespread exposure of pigs to HEV at both individual and farm levels. Future studies are needed to assess the factors associated with the risk of HEV presence on farms, with the aim to prevent virus introduction and spread within farms, thereby eliminating the risk at slaughterhouse.
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- 2023
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37. Heart Failure with Reduced Ejection Fraction—Does Sex Matter?
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Swaraj, Sascha, Kozor, Rebecca, Arnott, Clare, Di Bartolo, Belinda A., and A. Figtree, Gemma
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- 2021
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38. Revising Biomimetics: Opportunities and Ambiguities in the Bioinspired Design Approach
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Carla Langella, Amilton J. V. Arruda, and Carmelo Di Bartolo
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Mechanical drawing. Engineering graphics ,T351-385 - Abstract
The paper aims to propose an overview of biomimetic design, exploring its features from a methodological and critical point of view, highlighting its potential but also its limits, ambiguities, risks, and obscurities. Critical interpretations of biomimetic design are presented through a method based on comparative literature review. This was filtered by several crucial questions aimed at highlighting the effectiveness and value of design approaches based on the observation and emulation of nature in our current historical period. Semantic choices, market impact, efficiency of methods, interdisciplinary collaboration, ethical and environmental consequences are some of the issues analysed. These themes have been selected taking into consideration open and unresolved matters within the international state of the art, in order to trace possible evolutionary strategies for the biomimetic approach, relating it to the current social, economic, political and technological scenario.
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- 2022
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39. Development and evaluation of low-volume tests to detect and characterize antibodies to SARS-CoV-2
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Alice Halliday, Anna E. Long, Holly E. Baum, Amy C. Thomas, Kathryn L. Shelley, Elizabeth Oliver, Kapil Gupta, Ore Francis, Maia Kavanagh Williamson, Natalie Di Bartolo, Matthew J. Randell, Yassin Ben-Khoud, Ilana Kelland, Georgina Mortimer, Olivia Ball, Charlie Plumptre, Kyla Chandler, Ulrike Obst, Massimiliano Secchi, Lorenzo Piemonti, Vito Lampasona, Joyce Smith, Michaela Gregorova, Lea Knezevic, Jane Metz, Rachael Barr, Begonia Morales-Aza, Jennifer Oliver, Lucy Collingwood, Benjamin Hitchings, Susan Ring, Linda Wooldridge, Laura Rivino, Nicholas Timpson, Jorgen McKernon, Peter Muir, Fergus Hamilton, David Arnold, Derek N. Woolfson, Anu Goenka, Andrew D. Davidson, Ashley M. Toye, Imre Berger, Mick Bailey, Kathleen M. Gillespie, Alistair J. K. Williams, and Adam Finn
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antibody ,diagnostic ,ELISA ,evaluation ,SARS-CoV-2 ,COVID-19 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Low-volume antibody assays can be used to track SARS-CoV-2 infection rates in settings where active testing for virus is limited and remote sampling is optimal. We developed 12 ELISAs detecting total or antibody isotypes to SARS-CoV-2 nucleocapsid, spike protein or its receptor binding domain (RBD), 3 anti-RBD isotype specific luciferase immunoprecipitation system (LIPS) assays and a novel Spike-RBD bridging LIPS total-antibody assay. We utilized pre-pandemic (n=984) and confirmed/suspected recent COVID-19 sera taken pre-vaccination rollout in 2020 (n=269). Assays measuring total antibody discriminated best between pre-pandemic and COVID-19 sera and were selected for diagnostic evaluation. In the blind evaluation, two of these assays (Spike Pan ELISA and Spike-RBD Bridging LIPS assay) demonstrated >97% specificity and >92% sensitivity for samples from COVID-19 patients taken >21 days post symptom onset or PCR test. These assays offered better sensitivity for the detection of COVID-19 cases than a commercial assay which requires 100-fold larger serum volumes. This study demonstrates that low-volume in-house antibody assays can provide good diagnostic performance, and highlights the importance of using well-characterized samples and controls for all stages of assay development and evaluation. These cost-effective assays may be particularly useful for seroprevalence studies in low and middle-income countries.
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- 2022
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40. Complete intra-laboratory validation of a LAL assay for bacterial endotoxin determination in EBV-specific cytotoxic T lymphocytes
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Salvatore Pasqua, Maria Concetta Niotta, Giuseppina Di Martino, Davide Sottile, Bruno Douradinha, Monica Miele, Francesca Timoneri, Mariangela Di Bella, Nicola Cuscino, Chiara Di Bartolo, Pier Giulio Conaldi, and Danilo D’Apolito
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advanced therapy medicinal products ,ATMPs ,endotoxins ,qualification ,method validation ,Limulus amebocyte lysate ,Genetics ,QH426-470 ,Cytology ,QH573-671 - Abstract
Endotoxin content is a critical factor that affects the safety of biological pharmaceutical products. International pharmacopoeias describe several reference methods to determine endotoxin levels in advanced therapy medicinal product (ATMP) preparations. Administration of ATMPs must be done as rapidly as possible to ensure complete viability and potency of the cellular product. To evaluate the endotoxin content in the shortest time possible, we chose to validate an alternative method based on the use of the Charles River Portable Testing System (PTS) and FDA-approved cartridges, compliant with the requirements of the European Pharmacopoeia and providing results in
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- 2021
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41. Possible Human-to-Dog Transmission of SARS-CoV-2, Italy, 2020
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Nicola Decaro, Gabriele Vaccari, Alessio Lorusso, Eleonora Lorusso, Luca De Sabato, Edward I. Patterson, Ilaria Di Bartolo, Grant L. Hughes, Liana Teodori, Costantina Desario, Barbara Colitti, Dominga Ricci, Domenico Buonavoglia, Sergio Rosati, Vito Martella, Cesare Cammà, Umberto Agrimi, and Gabriella Elia
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dogs ,humans ,SARS-CoV-2 transmission ,next-generation sequencing ,phylogeny ,SARS-CoV-2 ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We detected severe acute respiratory syndrome coronavirus 2 in an otherwise healthy poodle living with 4 family members who had coronavirus disease. We observed antibodies in serum samples taken from the dog, indicating seroconversion. Full-length genome sequencing showed that the canine and human viruses were identical, suggesting human-to-animal transmission.
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- 2021
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42. Detection of HEV RNA Using One-Step Real-Time RT-PCR in Farrow-to-Finish Pig Farms in Bulgaria
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Gergana Lyubomirova Krumova-Valcheva, Ilaria Di Bartolo, Richard Piers Smith, Eva Gyurova, Gergana Mateva, Mihail Milanov, Albena Dimitrova, Elke Burow, and Hristo Daskalov
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hepatitis E virus ,farrow-to-finish pig farms ,finishers ,dry sows ,gilts ,one-step real-time RT-PCR ,Medicine - Abstract
(1) Background: HEV is a zoonotic, foodborne pathogen. It is spread worldwide and represents a public health risk. The aim of this study was to evaluate the presence of HEV RNA in farrow-to-finish pig farms in different regions of Bulgaria; (2) Methods: Isolation of HEV RNA from pooled samples of feces was performed using a QIAamp® Viral RNA Mini Kit followed by HEV RNA detection using a single-step real-time RT-PCR with primers and probes targeting the ORF 3 HEV genome; (3) Results: HEV RNA was detected in 12 out of 32 tested farms in Bulgaria (37.5%). The overall percentage of HEV-positive pooled fecal samples was 10.8% (68 of 630 samples). HEV was detected mostly in pooled fecal samples from finisher pigs (66/320, 20.6%) and sporadically from dry sows (1/62, 1.6%) and gilts (1/248, 0.4%); (4) Conclusions: Our results confirm that HEV circulates in farrow-to-finish pig farms in Bulgaria. In our study, we found HEV RNA in pooled fecal samples from fattening pigs (4–6-months age), shortly before their transport to the slaughterhouse indicating a potential risk to public health. The possible circulation of HEV throughout pork production requires monitoring and containment measures.
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- 2023
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43. Economic Impact of COVID-19 Lockdown on Italian NHS: Focus on Diabetes Mellitus
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Torre E, Colombo GL, Di Matteo S, Martinotti C, Valentino MC, Rebora A, Cecoli F, Monti E, Galimberti M, Di Bartolo P, Gaggioli G, and Bruno GM
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covid-19 ,diabetes type ii ,diabetic complications ,quality of life ,economic impact ,Medicine (General) ,R5-920 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Enrico Torre,1 Giorgio Lorenzo Colombo,2 Sergio Di Matteo,3 Chiara Martinotti,3 Maria Chiara Valentino,3 Alberto Rebora,1 Francesca Cecoli,1 Eleonora Monti,1 Marco Galimberti,4 Paolo Di Bartolo,5 Germano Gaggioli,6 Giacomo Matteo Bruno2 1Endocrinology Diabetology and Metabolic Diseases Unit - ASL3, Genoa, Italy; 2Department of Drug Sciences, University of Pavia, Pavia, Italy; 3S.A.V.E. Studi Analisi Valutazioni Economiche S.r.l., Health Economics & Outcomes Research, Milan, Italy; 4DP Trade SA, Lugano, Switzerland; 5Diabetes Center of Ravenna, Romagna Diabetes Clinical Network, Romagna Local Health Authority, Ravenna, Italy; 6Cardiology Unit Villascassi Hospital - ASL3, Genoa, ItalyCorrespondence: Giorgio Lorenzo ColomboDepartment of Drug Sciences, School of Pharmacy, University of Pavia, Pavia, ItalyEmail giorgio.colombo@unipv.itBackground: In Italy, the adoption of a total lockdown has generated almost total suspension of outpatient visits except for emergencies. Even after lockdown, the pandemic fear created additional barriers to access the health services. The aim of our study is to evaluate the economic impact of the lockdown for COVID-19 on public health in Italy, focusing on its effects on diabetic population.Materials and Methods: We analyzed the impact of the lockdown on excess mortality and morbidity in the Italian diabetic population during 2020. The analysis was divided into several steps: a quantification of specialist visit reduction, the calculation of excess mortality in the diabetic population, the economic evaluation of the slowdown in the use of innovative diabetic therapies. Furthermore, the impact of the lockdown on the reduction of procedures and follow-up visits in diabetic population was evaluated. The overall impact of the pandemic and lockdown effects on costs and quality of life was then calculated.Results: During 2020, a drop of 28% in patient access has been observed. Diabetic patients recorded a twice higher mortality value compared to general population (20.4% vs 10.2%). The analysis of market data revealed a slowdown in consumption of new antidiabetic therapies (− 14%, 27% vs 41%). We estimated an expense of € 26.6 million for NHS and a loss of 257 utilities in diabetic population due to the missed benefits related to slowdown in innovative antidiabetic drugs use and non-optimal follow-up and control of diabetes complications. In simulation scenarios, we also estimated an overall expenditure ranging from € 38.7 to 94.0 million and a loss of 294– 836 utilities.Conclusion: Diabetic population paid a high tribute to pandemic and lockdown, both in terms of number of deaths and burden of diabetic complications, together with an overall deterioration of quality of life.Keywords: COVID-19, diabetes type II, diabetic complications, quality of life, economic impact
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- 2021
44. Condizioni per collaborare. Scuole e terzo settore nella costruzione della comunità educante
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Luca Fanelli and Antonella Di Bartolo
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organizzazione scolastica ,comunità educante ,terzo settore ,Education (General) ,L7-991 ,Science ,Theory and practice of education ,LB5-3640 - Abstract
Il presente contributo parte dalle specifiche esperienze dei progetti OpenSpace e FA.C.E. e dal processo Lab Sperone Children per esplorare i caratteri del rapporto tra scuola e terzo settore a livello territoriale, contestualizzandolo nelle vicende che configurano l’attuale scenario. Procede quindi puntualizzando alcuni apprendimenti e proposte riguardanti, da un lato, la creazione di figure dedicate al lavoro di rete e, dall’altro, alla configurazione istituzionale nella quale le alleanze educative territoriali possono prosperare. Nelle conclusioni si tratteggiano brevemente alcune ulteriori condizioni necessarie affinché ciò possa verificarsi.
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- 2022
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45. Clinical profiles and quality of care of subjects with type 2 diabetes according to their cardiovascular risk: an observational, retrospective study
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Basilio Pintaudi, Alessia Scatena, Gabriella Piscitelli, Vera Frison, Salvatore Corrao, Valeria Manicardi, Giusi Graziano, Maria Chiara Rossi, Marco Gallo, Domenico Mannino, Paolo Di Bartolo, and Antonio Nicolucci
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Cardiovascular risk ,Type 2 diabetes ,Quality of care ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background The European Society of Cardiology (ESC) recently defined cardiovascular risk classes for subjects with diabetes. Aim of this study was to explore the distribution of subjects with type 2 diabetes (T2D) by cardiovascular risk groups according to the ESC classification and to describe the quality indicators of care, with particular regard to cardiovascular risk factors. Methods The study is based on data extracted from electronic medical records of patients treated at the 258 Italian diabetes centers participating in the AMD Annals initiative. Patients with T2D were stratified by cardiovascular risk. General descriptive indicators, measures of intermediate outcomes, intensity/appropriateness of pharmacological treatment for diabetes and cardiovascular risk factors, presence of other complications and overall quality of care were evaluated. Results Overall, 473,740 subjects with type 2 diabetes (78.5% at very high cardiovascular risk, 20.9% at high risk and 0.6% at moderate risk) were evaluated. Among people with T2D at very high risk: 26.4% had retinopathy, 39.5% had albuminuria, 18.7% had a previous major cardiovascular event, 39.0% had organ damage, 89.1% had three or more risk factors. The use of DPP4-i markedly increased as cardiovascular risk increased. The prescription of secretagogues also increased and that of GLP1-RAs tended to increase. The use of SGLT2-i was still limited, and only slightly higher in subjects with very high cardiovascular risk. The overall quality of care, as summarized by the Q score, tended to be lower as the level of cardiovascular risk increased. Conclusions A large proportion of subjects with T2D is at high or very high risk. Glucose-lowering drug therapies seem not to be adequately used with respect to their potential advantages in terms of cardiovascular risk reduction. Several actions are necessary to improve the quality of care.
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- 2021
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46. Use of 27G needles improves sensitivity and performance of ATCC anaerobe reference microorganism detection in BacT/Alert system
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Salvatore Pasqua, Giampiero Vitale, Anna Pasquariello, Bruno Douradinha, Fabio Tuzzolino, Francesca Cardinale, Chiara Cusimano, Chiara Di Bartolo, Pier Giulio Conaldi, and Danilo D’Apolito
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microbiological contaminations ,automated blood culture system ,BacT/Alert ,sterility testing ,microorganisms ,hospital infections ,Genetics ,QH426-470 ,Cytology ,QH573-671 - Abstract
Effective detection of microbiological contaminations present in medicinal cellular products is a crucial step to ensure patients’ safety. In recent decades, several rapid microbiological methods have been developed and validated, but variabilities linked to the use of different resources have led to discordant validation of methods and performance results. Considering this, while developing an in-house BacT/Alert-based method, we evaluated all of the materials used in its validation. Of particular importance, we noticed that the syringe gauge used to inject the samples into the bottles was crucial to obtain robust results. We chose to conduct a comparative test between the BacT/Alert system and the compendial method described in the European Pharmacopoeia, using five dilutions of nine reference microorganism strains and 21G or 27G needles. Our results confirmed that the BacT/Alert system is a valid and faster alternative method to assess sterility of clinical cell therapy products, and that the use of 27G needles increases its sensitivity to detect reference anaerobe microorganisms.
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- 2021
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47. Sex-Related Differences and Factors Associated with Peri-Procedural and 1 Year Mortality in Chronic Limb-Threatening Ischemia Patients from the CLIMATE Italian Registry
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Eugenio Martelli, Matilde Zamboni, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe M. Sangiorgi, Mariangela V. Puci, Allegra R. Martelli, Teresa Messina, Paolo Frigatti, Maria Pia Borrelli, Carlo Ruotolo, Ilaria Ficarelli, Paolo Rubino, Francesco Pezzo, Luciano Carbonari, Andrea Angelini, Edoardo Galeazzi, Luca Calia Di Pinto, Franco M. Fiore, Armando Palmieri, Giorgio Ventoruzzo, Giulia Mazzitelli, Franco Ragni, Antonio Bozzani, Enzo Forliti, Claudio Castagno, Pietro Volpe, Mafalda Massara, Diego Moniaci, Elisa Pagliasso, Tania Peretti, Mauro Ferrari, Nicola Troisi, Piero Modugno, Maurizio Maiorano, Umberto M. Bracale, Marco Panagrosso, Mario Monaco, Giovanni Giordano, Giuseppe Natalicchio, Antonella Biello, Giovanni M. Celoria, Alessio Amico, Mauro Di Bartolo, Massimiliano Martelli, Roberta Munaó, Davide Razzano, Giovanni Colacchio, Francesco Bussetti, Gaetano Lanza, Antonio Cardini, Bartolomeo Di Benedetto, Mario De Laurentis, Maurizio Taurino, Pasqualino Sirignano, Pierluigi Cappiello, Andrea Esposito, Santi Trimarchi, Silvia Romagnoli, Andrea Padricelli, Giorgio Giudice, Adolfo Crinisio, Giovanni Di Nardo, Giuseppe Battaglia, Rosario Tringale, Salvatore De Vivo, Rita Compagna, Valerio S. Tolva, Ilenia D’Alessio, Ruggiero Curci, Simona Giovannetti, Giuseppe D’Arrigo, Giusi Basile, Dalmazio Frigerio, Gian Franco Veraldi, Luca Mezzetto, Arnaldo Ippoliti, Fabio M. Oddi, and Alberto M. Settembrini
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chronic limb-threatening ischemia ,outcome ,sex ,age ,limb salvage ,Medicine - Abstract
Background: Identifying sex-related differences/variables associated with 30 day/1 year mortality in patients with chronic limb-threatening ischemia (CLTI). Methods: Multicenter/retrospective/observational study. A database was sent to all the Italian vascular surgeries to collect all the patients operated on for CLTI in 2019. Acute lower-limb ischemia and neuropathic-diabetic foot are not included. Follow-up: One year. Data on demographics/comorbidities, treatments/outcomes, and 30 day/1 year mortality were investigated. Results: Information on 2399 cases (69.8% men) from 36/143 (25.2%) centers. Median (IQR) age: 73 (66–80) and 79 (71–85) years for men/women, respectively (p < 0.0001). Women were more likely to be over 75 (63.2% vs. 40.1%, p = 0.0001). More men smokers (73.7% vs. 42.2%, p < 0.0001), are on hemodialysis (10.1% vs. 6.7%, p = 0.006), affected by diabetes (61.9% vs. 52.8%, p < 0.0001), dyslipidemia (69.3% vs. 61.3%, p < 0.0001), hypertension (91.8% vs. 88.5%, p = 0.011), coronaropathy (43.9% vs. 29.4%, p < 0.0001), bronchopneumopathy (37.1% vs. 25.6%, p < 0.0001), underwent more open/hybrid surgeries (37.9% vs. 28.8%, p < 0.0001), and minor amputations (22% vs. 13.7%, p < 0.0001). More women underwent endovascular revascularizations (61.6% vs. 55.2%, p = 0.004), major amputations (9.6% vs. 6.9%, p = 0.024), and obtained limb-salvage if with limited gangrene (50.8% vs. 44.9%, p = 0.017). Age > 75 (HR = 3.63, p = 0.003) is associated with 30 day mortality. Age > 75 (HR = 2.14, p < 0.0001), nephropathy (HR = 1.54, p < 0.0001), coronaropathy (HR = 1.26, p = 0.036), and infection/necrosis of the foot (dry, HR = 1.42, p = 0.040; wet, HR = 2.04, p < 0.0001) are associated with 1 year mortality. No sex-linked difference in mortality statistics. Conclusion: Women exhibit fewer comorbidities but are struck by CLTI when over 75, a factor associated with short- and mid-term mortality, explaining why mortality does not statistically differ between the sexes.
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- 2023
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48. Absence of Hepatitis E Virus (HEV) in Italian Lagomorph Species Sampled between 2019 and 2021
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Luca De Sabato, Giovanni Ianiro, Virginia Filipello, Sara Arnaboldi, Francesco Righi, Fabio Ostanello, Monica Giammarioli, Antonio Lavazza, and Ilaria Di Bartolo
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hepatitis E virus ,rabbit ,hare ,wild rabbit ,zoonosis ,HEV ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
The zoonotic hepatitis E virus genotype 3 (HEV-3) causes most autochthonous human hepatitis E cases in Europe, which are due to the consumption of raw or undercooked food products of animal origin. Pigs and wild boars are considered the main reservoirs of this genotype, while rabbits are the reservoir of a distinct phylogenetic group named HEV-3ra, which is classified within the HEV-3 genotype but in a separate clade. Evidence for the zoonotic potential of HEV-3ra was suggested by its detection in immunocompromised patients in several European countries. HEV-3ra infection was found in farmed and feral rabbit populations worldwide and its circulation was reported in a few European countries, including Italy. Furthermore, Italy is one of the major rabbit meat producers and consumers across Europe, but only a few studies investigated the presence of HEV in this reservoir. The aim of this study was to assess the presence of HEV in 328 Italian hares and 59 farmed rabbits collected in 3 Italian macro-areas (North, North-Central, and South-Central), between 2019 and 2021. For this purpose, liver samples were used to detect HEV RNA using broad-range real-time RT-PCR and nested RT-PCR. Using 28 liver transudates from hares, the ELISA test for anti-HEV IgG detection was also performed. Neither HEV RNA nor anti-HEV antibodies were detected. Further studies will be conducted to assess the HEV presence in Italian lagomorphs to establish the role of this host and the possible risk of transmission for workers with occupational exposure, to pet owners and via food.
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- 2023
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49. In Vitro Replication of Swine Hepatitis E Virus (HEV): Production of Cell-Adapted Strains
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Giovanni Ianiro, Marina Monini, Maria Grazia Ammendolia, Luca De Sabato, Fabio Ostanello, Gabriele Vaccari, and Ilaria Di Bartolo
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HEV ,subtype ,HEV-3 ,A549 ,isolate ,cell culture ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
The hepatitis E caused by the virus HEV of genotypes HEV-3 and HEV-4 is a zoonotic foodborne disease spread worldwide. HEV is currently classified into eight different genotypes (HEV-1–8). Genotypes HEV-3 and HEV-4 are zoonotic and are further divided into subtypes. Most of the information on HEV replication remains unknown due to the lack of an efficient cell cultivation system. Over the last couple of years, several protocols for HEV cultivation have been developed on different cell lines; even if they were troublesome, long, and scarcely reproducible, they offered the opportunity to study the replicative cycle of the virus. In the present study, we aimed to obtain a protocol ready to use viral stock in serum free medium that can be used with reduced time of growth and without any purification steps. The employed method allowed isolation and cell adaptation of four swine HEV-3 strains, belonging to three different subtypes. Phylogenetic analyses conducted on partial genome sequences of in vitro isolated strains did not reveal any insertion in the hypervariable region (HVR) of the genomes. A limited number of mutations was acquired in the genome during the virus growth in the partial sequences of Methyltransferase (Met) and ORF2 coding genes.
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- 2023
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50. Delphi-Based Consensus on Treatment Intensification in Type 2 Diabetes Subjects Failing Basal Insulin Supported Oral Treatment: Focus on Basal Insulin + GLP-1 Receptor Agonist Combination Therapies
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Fadini, Gian Paolo, Disoteo, Olga, Candido, Riccardo, Di Bartolo, Paolo, Laviola, Luigi, and Consoli, Agostino
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- 2021
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