90 results on '"P. Froissard"'
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2. Actor-critic versus direct policy search: a comparison based on sample complexity
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de Broissia, Arnaud de Froissard and Sigaud, Olivier
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Computer Science - Learning - Abstract
Sample efficiency is a critical property when optimizing policy parameters for the controller of a robot. In this paper, we evaluate two state-of-the-art policy optimization algorithms. One is a recent deep reinforcement learning method based on an actor-critic algorithm, Deep Deterministic Policy Gradient (DDPG), that has been shown to perform well on various control benchmarks. The other one is a direct policy search method, Covariance Matrix Adaptation Evolution Strategy (CMA-ES), a black-box optimization method that is widely used for robot learning. The algorithms are evaluated on a continuous version of the mountain car benchmark problem, so as to compare their sample complexity. From a preliminary analysis, we expect DDPG to be more sample efficient than CMA-ES, which is confirmed by our experimental results., Comment: Proceedings JFPDA (Journees Francaises Planification Decision Apprentissage)
- Published
- 2016
3. Oil Bodies from Chia (Salvia hispanica L.) and Camelina (Camelina sativa L.) Seeds for Innovative Food Applications: Microstructure, Composition and Physical Stability
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Christelle Lopez, Hélène Sotin, Hanitra Rabesona, Bruno Novales, Jean-Michel Le Quéré, Marine Froissard, Jean-Denis Faure, Sylvain Guyot, and Marc Anton
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lipid droplet ,oil body ,interface ,membrane ,natural oil-in-water emulsion ,plant-based food ,Chemical technology ,TP1-1185 - Abstract
Exploring and deciphering the biodiversity of oil bodies (OBs) recovered from oilseeds are of growing interest in the preparation of sustainable, natural and healthy plant-based food products. This study focused on chia (Salvia hispanica L.) and camelina (Camelina sativa L.) seed OBs. A green refinery process including ultrasound to remove mucilage, aqueous extraction by grinding and centrifugation to recover OBs from the seeds was used. The microstructure, composition and physical stability of the OBs were examined. Confocal laser scanning microscopy images showed that chia and camelina seed OBs are spherical assemblies coated by a layer of phospholipids and proteins, which have been identified by gel electrophoresis. The mean diameters determined by laser light scattering measurements were 2.3 and 1.6 µm for chia and camelina seed OBs, respectively. The chia and camelina seed OBs were rich in lipids and other bioactive components with, respectively, 64% and 30% α-linolenic acid representing 70% and 53% of the total fatty acids in the sn-2 position of the triacylglycerols, 0.23% and 0.26% phospholipids, 3069 and 2674 mg/kg oil of β-sitosterol, and lipophilic antioxidants: 400 and 670 mg/kg oil of γ-tocopherol. Phenolic compounds were recovered from the aqueous extracts, such as rutin from camelina and caffeic acid from chia. Zeta-potential measurements showed changes from about −40 mV (pH 9) to values that were positive below the isoelectric points of pH 5.1 and 3.6 for chia and camelina seed OBs, respectively. Below pH 6.5, physical instability of the natural oil-in-water emulsions with aggregation and phase separation was found. This study will contribute to the development of innovative and sustainable food products based on natural oil-in-water emulsions containing chia and camelina seed OBs for their nutritional and health benefits.
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- 2023
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4. The Host Protein Aquaporin-9 is Required for Efficient Plasmodium falciparum Sporozoite Entry into Human Hepatocytes
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Nadia Amanzougaghene, Shahin Tajeri, Samir Yalaoui, Audrey Lorthiois, Valérie Soulard, Audrey Gego, Armelle Rametti, Véronica Risco-Castillo, Alicia Moreno, Maurel Tefit, Geert-Jan van Gemert, Robert W. Sauerwein, Jean-Christophe Vaillant, Philippe Ravassard, Jean-Louis Pérignon, Patrick Froissard, Dominique Mazier, and Jean-François Franetich
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Plasmodium falciparum ,sporozoites ,liver stage ,hepatocytes ,Aquaporin-9 ,CD81 ,Microbiology ,QR1-502 - Abstract
Hepatocyte invasion by Plasmodium sporozoites represents a promising target for innovative antimalarial therapy, but the molecular events mediating this process are still largely uncharacterized. We previously showed that Plasmodium falciparum sporozoite entry into hepatocytes strictly requires CD81. However, CD81-overexpressing human hepatoma cells remain refractory to P. falciparum infection, suggesting the existence of additional host factors necessary for sporozoite entry. Here, through differential transcriptomic analysis of human hepatocytes and hepatoma HepG2-CD81 cells, the transmembrane protein Aquaporin-9 (AQP9) was found to be among the most downregulated genes in hepatoma cells. RNA silencing showed that sporozoite invasion of hepatocytes requires AQP9 expression. AQP9 overexpression in hepatocytes increased their permissiveness to P. falciparum. Moreover, chemical disruption with the AQP9 inhibitor phloretin markedly inhibited hepatocyte infection. Our findings identify AQP9 as a novel host factor required for P. falciparum sporozoite hepatocyte-entry and indicate that AQP9 could be a potential therapeutic target.
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- 2021
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5. Regulation of lipid droplet dynamics in Saccharomyces cerevisiae depends on the Rab7-like Ypt7p, HOPS complex and V1-ATPase
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Isabelle Bouchez, Marie Pouteaux, Michel Canonge, Mélanie Genet, Thierry Chardot, Alain Guillot, and Marine Froissard
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Ypt7p ,Rab GTPase ,Lipid droplet ,Vacuole ,V-ATPase ,Science ,Biology (General) ,QH301-705.5 - Abstract
It has now been clearly shown that lipid droplets (LDs) play a dynamic role in the cell. This was reinforced by LD proteomics which suggest that a significant number of trafficking proteins are associated with this organelle. Using microscopy, we showed that LDs partly co-localize with the vacuole in S. cerevisiae. Immunoblot experiments confirmed the association of the vacuolar Rab GTPase Rab7-like Ypt7p with LDs. We observed an increase in fatty acid content and LD number in ypt7Δ mutant and also changes in LD morphology and intra LD fusions, revealing a direct role for Ypt7p in LD dynamics. Using co-immunoprecipitation, we isolated potential Ypt7p partners including, Vma13p, the H subunit of the V1 part of the vacuolar (H+) ATPase (V-ATPase). Deletion of the VMA13 gene, as well as deletion of three other subunits of the V1 part of the V-ATPase, also increased the cell fatty acid content and LD number. Mutants of the Homotypic fusion and vacuole protein sorting (HOPS) complex showed similar phenotypes. Here, we demonstrated that LD dynamics and membrane trafficking between the vacuole and LDs are regulated by the Rab7-like Ypt7p and are impaired when the HOPS complex and the V1 domain of the V-ATPase are defective.
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- 2015
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6. Bioconversion of agricultural lignocellulosic residues into branched-chain fatty acids using Streptomyces lividans
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Dulermo Thierry, Coze Fabien, Virolle Marie-Joëlle, Méchin Valérie, Baumberger Stéphanie, and Froissard Marine
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Streptomyces ,rapeseed ,sunflower ,fatty acids ,lignocellulose ,Oils, fats, and waxes ,TP670-699 - Abstract
Two lignocellulosic agricultural residues, sunflower stalks and rape straw, were investigated as potential low-cost, non-food substrates for the production of triacylglycerols by the oleaginous, lignocellulolytic bacteria Streptomyces lividans. Chemical analysis of each type of residue revealed similar cell wall compositions in the polysaccharides and lignins of the two feedstocks, with high lignin β-O-4 bond content compared to other angiosperms’ lignin. Growing tests of Streptomyces lividans TK 24 were performed before and after sequential water and ethanol extraction by assessing bacterial fatty acid accumulation. All extracted and non-extracted samples were found to be substrates of the bacteria with fatty acid production ranging between 19% and 44% of the production obtained with arabinose as a reference substrate. The maximum conversion rate was obtained with the less lignified, non-extracted sample. This study suggests that lignocellulosic residues from oleaginous crops could be advantageously valorized by microbial bioconversion processes for the production of lipids of interest.
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- 2016
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7. From near-surface to root-zone soil moisture using an exponential filter: an assessment of the method based on in-situ observations and model simulations
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C. Albergel, C. Rüdiger, T. Pellarin, J.-C. Calvet, N. Fritz, F. Froissard, D. Suquia, A. Petitpa, B. Piguet, and E. Martin
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Technology ,Environmental technology. Sanitary engineering ,TD1-1066 ,Geography. Anthropology. Recreation ,Environmental sciences ,GE1-350 - Abstract
A long term data acquisition effort of profile soil moisture is under way in southwestern France at 13 automated weather stations. This ground network was developed in order to validate remote sensing and model soil moisture estimates. In this paper, both those in situ observations and a synthetic data set covering continental France are used to test a simple method to retrieve root zone soil moisture from a time series of surface soil moisture information. A recursive exponential filter equation using a time constant, T, is used to compute a soil water index. The Nash and Sutcliff coefficient is used as a criterion to optimise the T parameter for each ground station and for each model pixel of the synthetic data set. In general, the soil water indices derived from the surface soil moisture observations and simulations agree well with the reference root-zone soil moisture. Overall, the results show the potential of the exponential filter equation and of its recursive formulation to derive a soil water index from surface soil moisture estimates. This paper further investigates the correlation of the time scale parameter T with soil properties and climate conditions. While no significant relationship could be determined between T and the main soil properties (clay and sand fractions, bulk density and organic matter content), the modelled spatial variability and the observed inter-annual variability of T suggest that a weak climate effect may exist.
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- 2008
8. Single cell synchrotron FT-IR microspectroscopy reveals a link between neutral lipid and storage carbohydrate fluxes in S. cerevisiae.
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Frédéric Jamme, Jean-David Vindigni, Valérie Méchin, Tamazight Cherifi, Thierry Chardot, and Marine Froissard
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Medicine ,Science - Abstract
In most organisms, storage lipids are packaged into specialized structures called lipid droplets. These contain a core of neutral lipids surrounded by a monolayer of phospholipids, and various proteins which vary depending on the species. Hydrophobic structural proteins stabilize the interface between the lipid core and aqueous cellular environment (perilipin family of proteins, apolipoproteins, oleosins). We developed a genetic approach using heterologous expression in Saccharomyces cerevisiae of the Arabidopsis thaliana lipid droplet oleosin and caleosin proteins AtOle1 and AtClo1. These transformed yeasts overaccumulate lipid droplets, leading to a specific increase in storage lipids. The phenotype of these cells was explored using synchrotron FT-IR microspectroscopy to investigate the dynamics of lipid storage and cellular carbon fluxes reflected as changes in spectral fingerprints. Multivariate statistical analysis of the data showed a clear effect on storage carbohydrates and more specifically, a decrease in glycogen in our modified strains. These observations were confirmed by biochemical quantification of the storage carbohydrates glycogen and trehalose. Our results demonstrate that neutral lipid and storage carbohydrate fluxes are tightly connected and co-regulated.
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- 2013
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9. Natural variation in seed very long chain fatty acid content is controlled by a new isoform of KCS18 in Arabidopsis thaliana.
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Sophie Jasinski, Alain Lécureuil, Martine Miquel, Olivier Loudet, Sylvain Raffaele, Marine Froissard, and Philippe Guerche
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Medicine ,Science - Abstract
Oil from oleaginous seeds is mainly composed of triacylglycerols. Very long chain fatty acids (VLCFAs) are major constituents of triacylglycerols in many seed oils and represent valuable feedstock for industrial purposes. To identify genetic factors governing natural variability in VLCFA biosynthesis, a quantitative trait loci (QTL) analysis using a recombinant inbred line population derived from a cross between accessions Bay-0 and Shahdara was performed in Arabidopsis thaliana. Two fatty acid chain length ratio (CLR) QTL were identified, with one major locus, CLR.2, accounting for 77% of the observed phenotypic variation. A fine mapping and candidate gene approach showed that a key enzyme of the fatty acid elongation pathway, the β-ketoacyl-CoA synthase 18 (KCS18), was responsible for the CLR.2 QTL detected between Bay-0 and Shahdara. Association genetics and heterologous expression in yeast cells identified a single point mutation associated with an alteration of KCS18 activity, uncovering the molecular bases for the modulation of VLCFA content in these two natural populations of Arabidopsis. Identification of this kcs18 mutant with altered activity opens new perspectives for the modulation of oil composition in crop plants.
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- 2012
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10. Populations of Myriophyllum alterniflorum L. as bioindicators of pollution in acidic to neutral rivers in the Limousin region
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Chatenet, P., Froissard, D., Cook-Moreau, J., Hourdin, P., Ghestem, A., Botineau, M, and Haury, J.
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- 2006
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11. Copper Accumulation in a Reservoir Ecosystem Following Copper Sulfate Treatment (St. Germain Les Belles, France)
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van Hullebusch, Eric, Chatenet, Philippe, Deluchat, Véronique, Chazal, Philippe M., Froissard, Didier, Botineau, Michel, Ghestem, Axel, and Baudu, Michel
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- 2003
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12. Temperature shift and host cell contact up-regulate sporozoite expression of Plasmodium falciparum genes involved in hepatocyte infection.
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Anthony Siau, Olivier Silvie, Jean-François Franetich, Samir Yalaoui, Carine Marinach, Laurent Hannoun, Geert-Jaan van Gemert, Adrian J F Luty, Emmanuel Bischoff, Peter H David, Georges Snounou, Catherine Vaquero, Patrick Froissard, and Dominique Mazier
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Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
Plasmodium sporozoites are deposited in the skin by Anopheles mosquitoes. They then find their way to the liver, where they specifically invade hepatocytes in which they develop to yield merozoites infective to red blood cells. Relatively little is known of the molecular interactions during these initial obligatory phases of the infection. Recent data suggested that many of the inoculated sporozoites invade hepatocytes an hour or more after the infective bite. We hypothesised that this pre-invasive period in the mammalian host prepares sporozoites for successful hepatocyte infection. Therefore, the genes whose expression becomes modified prior to hepatocyte invasion would be those likely to code for proteins implicated in the subsequent events of invasion and development. We have used P. falciparum sporozoites and their natural host cells, primary human hepatocytes, in in vitro co-culture system as a model for the pre-invasive period. We first established that under co-culture conditions, sporozoites maintain infectivity for an hour or more, in contrast to a drastic loss in infectivity when hepatocytes were not included. Thus, a differential transcriptome of salivary gland sporozoites versus sporozoites co-cultured with hepatocytes was established using a pan-genomic P. falciparum microarray. The expression of 532 genes was found to have been up-regulated following co-culture. A fifth of these genes had no orthologues in the genomes of Plasmodium species used in rodent models of malaria. Quantitative RT-PCR analysis of a selection of 21 genes confirmed the reliability of the microarray data. Time-course analysis further indicated two patterns of up-regulation following sporozoite co-culture, one transient and the other sustained, suggesting roles in hepatocyte invasion and liver stage development, respectively. This was supported by functional studies of four hitherto uncharacterized proteins of which two were shown to be sporozoite surface proteins involved in hepatocyte invasion, while the other two were predominantly expressed during hepatic parasite development. The genome-wide up-regulation of expression observed supports the hypothesis that the shift from the mosquito to the mammalian host contributes to activate quiescent salivary gland sporozoites into a state of readiness for the hepatic stages. Functional studies on four of the up-regulated genes validated our approach as one means to determine the repertoire of proteins implicated during the early events of the Plasmodium infection, and in this case that of P. falciparum, the species responsible for the severest forms of malaria.
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- 2008
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13. Hepatocyte permissiveness to Plasmodium infection is conveyed by a short and structurally conserved region of the CD81 large extracellular domain.
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Samir Yalaoui, Sergine Zougbédé, Stéphanie Charrin, Olivier Silvie, Cécile Arduise, Khemais Farhati, Claude Boucheix, Dominique Mazier, Eric Rubinstein, and Patrick Froissard
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Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
Invasion of hepatocytes by Plasmodium sporozoites is a prerequisite for establishment of a malaria infection, and thus represents an attractive target for anti-malarial interventions. Still, the molecular mechanisms underlying sporozoite invasion are largely unknown. We have previously reported that the tetraspanin CD81, a known receptor for the hepatitis C virus (HCV), is required on hepatocytes for infection by sporozoites of several Plasmodium species. Here we have characterized CD81 molecular determinants required for infection of hepatocytic cells by P. yoelii sporozoites. Using CD9/CD81 chimeras, we have identified in CD81 a 21 amino acid stretch located in a domain structurally conserved in the large extracellular loop of tetraspanins, which is sufficient in an otherwise CD9 background to confer susceptibility to P. yoelii infection. By site-directed mutagenesis, we have demonstrated the key role of a solvent-exposed region around residue D137 within this domain. A mAb that requires this region for optimal binding did not block infection, in contrast to other CD81 mAbs. This study has uncovered a new functionally important region of CD81, independent of HCV E2 envelope protein binding domain, and further suggests that CD81 may not interact directly with a parasite ligand during Plasmodium infection, but instead may regulate the function of a yet unknown partner protein.
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- 2008
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14. Structural organization of str 246C and str 246N, plant defense-related genes from Nicotiana tabacum
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Froissard, Didier, Gough, Clare, Czernic, Pierre, Schneider, Michel, Toppan, Alain, Roby, Dominique, and Marco, Yves
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- 1994
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15. Differential regulation in tobacco cell suspensions of genes involved in plant-bacteria interactions by pathogen-related signals
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Godiard, Laurence, Froissard, Didier, Fournier, Joëlle, Axelos, Michèle, and Marco, Yves
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- 1991
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16. Transcriptional activation of 2 classes of genes during the hypersensitive reaction of tobacco leaves infiltrated with an incompatible isolate of the phytopathogenic bacterium Pseudomonas solanacearum
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Marco, Yves J., Ragueh, Fatima, Godiard, Laurence, and Froissard, Didier
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- 1990
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17. The Tore Supra LHCD control system: new improvements using PLCs and state machines
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J Baudet, P Pastor, F. Kazarian, J.Y. Journeaux, J Simoncini, and P. Froissard
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Finite-state machine ,business.industry ,Computer science ,Mechanical Engineering ,Programmable logic controller ,Control engineering ,Tore Supra ,Software ,Data acquisition ,Nuclear Energy and Engineering ,Human interface device ,Control system ,General Materials Science ,business ,Real-time operating system ,Civil and Structural Engineering - Abstract
The Tore Supra lower hybrid (LH) control system has been in operation for 10 years. Although its control system has given excellent service, it is an ageing system, and incompatible with many modern components and techniques. Hence, in order to improve the LH system's reliability, efficiency, compatibility and visibility, a new design of its control system was undertaken. A solution based on modern programmable logic controller coupled with a fast logic hardware ‘state machine’ has been selected as a replacement. This can handle the ‘state diagram’ and associated safety interlock functions for each klystron with the required time response. This paper presents the association of this state machine for fast control with the PLC for slow control, a PC based human interface, and all the control and process diagnostic functions which complete this Tore Supra LH control and security system.
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- 2001
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18. Coupling and power handling of the new Tore Supra LHCD launcher
- Author
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E. Bertrand, F. Kazarian, S. Dutheil, L. Tanaskovic, Ph. Bibet, A. Ekedahl, and P. Froissard
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Physics ,Electron density ,business.industry ,Plasma parameters ,Mechanical Engineering ,Near and far field ,Electron ,Tore Supra ,Fusion power ,Nuclear physics ,Optics ,Nuclear Energy and Engineering ,Electric field ,Limiter ,General Materials Science ,business ,Civil and Structural Engineering - Abstract
A new LHCD launcher, designed to inject 4 MW during 1000 s, has been installed on Tore Supra and successfully tested. A power of 3 MW with 7% power reflection coefficient has been reached. The coupling has been studied for various plasma parameters and positions, in order to study the robustness of the mode converter used in the antenna to divide the power into three in the poloidal direction. Due to the reduced electric field and the very efficiently water-cooled guard limiter, the temperature increase induced by the acceleration of electrons in the near field has been drastically reduced.
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- 2001
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19. Lower hybrid dynamics by ECE spectra in Tore Supra
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Yves Peysson, P Gomez, D. Thouvenin, P. Froissard, J. L. Segui, and G. Giruzzi
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Physics ,Mechanical Engineering ,Cyclotron resonance ,Tore Supra ,Lower hybrid oscillation ,Electron cyclotron resonance ,Nuclear Energy and Engineering ,Physics::Plasma Physics ,Electron temperature ,General Materials Science ,Plasma diagnostics ,Cyclotron radiation ,Atomic physics ,Diffusion (business) ,Civil and Structural Engineering - Abstract
The suprathermal electron cyclotron radiation is used to investigate fundamental phenomena governing electron tail formation during lower hybrid current drive (LHCD). To this end, an approximate analytical solution of the 2-D time-dependent Fokker-Planck equation is developed. Electron cyclotron emission (ECE) is analysed with this model. The pitch-angle scattering time, the characteristic time of the quasi-linear diffusion process, the perpendicular temperature and the quasi-linear diffusion coefficient are measured by fitting the temporal evolution of the ECE si,signal. (C) 2001 Elsevier Science B.V. All rights reserved.
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- 2001
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20. New advanced launcher for lower hybrid current drive on Tore Supra
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P. Hertout, A. Durocher, F. Surle, J.J. Cordier, M. Chantant, L. Garampon, L Garguiolo, A. Ekedahl, F. Kazarian, M. Goniche, C. Deck, Ph. Bibet, G. Rey, C. Portafaix, D Lafon, L. Doceul, G. Tonon, F. Samaille, P. Froissard, and G. Agarici
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Physics ,Mechanical Engineering ,Nuclear engineering ,Pulse duration ,Plasma ,Tore Supra ,Nuclear magnetic resonance ,Nuclear Energy and Engineering ,Limiter ,Torque ,General Materials Science ,Power flux ,Civil and Structural Engineering ,Power density - Abstract
A new actively cooled advanced launcher is being built for Tore Supra LHCD to inject 4 MW during 1000 s at 3.7 GHz, at a power density of 25 MW/m 2 (a conservative value observed in Tore Supra experiments). It is made from an array of 6×48 active and 6×9 passive waveguides. The design uses technologies which are relevant for a next step machine such that it can: (i) withstand a plasma radiated flux of 0.15 MW/m 2 ; (ii) radiate power with spectra having peak N// values of 2.02±0.35; (iii) withstand a total torque of 8.6 10 4 N m during disruptions; (iv) allow an antenna 20 cm radial stroke adjustable in real time, (v) withstand a convected power flux of 10 MW/m 2 on its guard limiter made of CFC tiles. A prototype of each new component of this antenna has been tested successfully at the nominal power with a pulse length of 1000 s.
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- 2000
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21. Enhanced heat flux in the scrape-off layer due to electrons accelerated in the near field of lower hybrid grills
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J. Mailloux, Ph. Jacquet, J. H. Harris, V. Fuchs, M. Goniche, P. Froissard, D. Guilhem, Y. Demers, P. Bibet, J.T. Hogan, G. Rey, and X. Litaudon
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Physics ,Nuclear and High Energy Physics ,Convective heat transfer ,business.industry ,Field line ,Near and far field ,Plasma ,Electron ,Tore Supra ,Condensed Matter Physics ,Computational physics ,Optics ,Heat flux ,Physics::Plasma Physics ,Landau damping ,business - Abstract
As a result of experimental observations of localized heat flux on components magnetically connected to radiating waveguides in Tore Supra and in TdeV, the acceleration of electrons near lower hybrid (LH) antennas has been investigated. A simple analytical model has been developed to compute the dynamics of the particles in the near field approximation. Landau damping of the very high N|| (20 < N|| < 100) component of the launched spectrum on the thermal electrons of the scrape-off layer (SOL) is found to occur. Simulation of a typical LH pulse in Tore Supra indicates that the electrons can be accelerated up to 2-3 keV. Modelling of the interaction of this fast electron population with the edge plasma allows a calculation of the heat flux on plasma facing components that are magnetically connected to the antenna. Model results and the results of experiments in Tore Supra and TdeV are compared. The calculated heat fluxes are found to be fairly consistent when the variation of convective heat flux at the grill aperture is taken into account. From this analysis, it is concluded that, for an LH power density of 25 MW/m2, the resulting heat flux along the field lines (3.5 MW/m2) is manageable for the components connected to the antenna, provided that good coupling can be maintained at a low density in front of the grill.
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- 1998
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22. Conditioning and high power operation of the lower hybrid current drive launcher in JET
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F. Rimini, B. Fischer, J. A. Romero, J.A. Dobbing, C. Gormezano, M. Lennholm, A. Ekedahl, P. Schildand, P. Froissard, F. X. Söldner, and P. Finburg
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Physics ,Power transmission ,Jet (fluid) ,Tokamak ,Divertor ,Nuclear engineering ,Joint European Torus ,Plasma ,Condensed Matter Physics ,Power (physics) ,law.invention ,Nuclear Energy and Engineering ,law ,Electric field - Abstract
The full lower hybrid current drive (LHCD) system on the Joint European Torus (JET) has been operational since the start of the pumped divertor phase of JET in 1994. By a campaign of vacuum conditioning in the beginning of operation, the power transmission and conditioning of the fuli launcher was speeded up, as compared with the prototype launcher used in 1990/91. After vents of the tokamak, the conditioning can be recovered by -300 vacuum pulses over 3-4 days. Even after extensive conditioning and with efficient pumping, the power handling of the fuli LHCD system on plasma is limited by the maximum electric field in the waveguides, the limit being 400-500 kV/m.
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- 1998
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23. Feedback control of the current profile on Tore Supra
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X. Litaudon, T. Wijnands, D. Van Houtte, G. Martin, and P. Froissard
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Physics ,Nuclear and High Energy Physics ,Steady state ,Safety factor ,Plasma ,Mechanics ,Current (fluid) ,Tore Supra ,Condensed Matter Physics ,Current density ,Partial current ,Voltage - Abstract
Recent results on feedback control of the global shape of the current density profile in discharges with lower hybrid current drive (LHCD) on Tore Supra are presented. The global shape of the current density profile is characterized by its internal inductance li, and feedback controlled through the LH power or the launched LH wave spectrum. Feedback control of the flux on the plasma boundary has allowed for exploration of regimes with partial current drive (constant loop voltage) and full current drive (zero loop voltage). In stationary, steady state discharges at 0.8 MA (qa=7), the current profile shape is characterized by an li between 1.5 and 1.7 and a safety factor on-axis (q0) between 1 and 1.4. The energy confinement is 1.3 to 1.6 times higher than the value predicted by the Rebut-Lallia-Watkins scaling law
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- 1997
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24. Strong toroidal asymmetries in power deposition on divertor and first wall components during LHCD on TdeV and Tore Supra
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G. Rey, J. Mailloux, J. P. Gunn, A. Côté, J.H. Harris, Y. Demers, M. Tareb, M. Goniche, P. Froissard, C. Boucher, Ph. Jacquet, C. Côté, V. Fuchs, Ph. Bibet, B. Terreault, and D. Guilhem
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Nuclear and High Energy Physics ,Toroid ,Chemistry ,Divertor ,RF power amplifier ,Analytical chemistry ,Near and far field ,Electron ,Tore Supra ,Computational physics ,Nuclear Energy and Engineering ,Physics::Plasma Physics ,Dielectric heating ,General Materials Science ,Antenna (radio) - Abstract
Strong toroidal asymmetries in power deposition during lower hybrid current drive experiments have been observed on TdeV and Tore Supra. These asymmetries are characterized by high heat loads on components magnetically connected to the LHCD antenna. The experimental results indicate that fast electrons generated by the rf fields in the scrape-off layer in front of the antenna are responsible for the localized heat loads. The rf power lost in the edge though this channel increases with both the edge density and the rf power level. In TdeV, the fraction of the injected power lost can exceed 10% at high power and high density, while it stays under 2% in Tore Supra. A simple model describing the trajectories of electrons in front of the antenna suggests that the high N| components of the near field spectrum are responsible for the acceleration of the scrape-off layer electrons.
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- 1997
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25. Runaway electron measurements in the JET tokamak
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R Martin Solis, P. van Belle, O.N. Jarvis, E Cecil, P Froissard, G.J. Sadler, B Fischer, B Esposito, N. Watkins, Raul Sanchez, J. M. Adams, and F.B. Marcus
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Physics ,Jet (fluid) ,Tokamak ,Turbulence ,Astrophysics::High Energy Astrophysical Phenomena ,Transport coefficient ,Bremsstrahlung ,Electron ,Plasma ,Condensed Matter Physics ,law.invention ,Magnetic field ,Nuclear Energy and Engineering ,law ,Atomic physics - Abstract
The perpendicular x-ray emission up a to few MeV of runaway electrons has been measured in JET low-density ohmic discharges by means of the fast electron bremsstrahlung profile monitor. A diffusion model simulating the temporal evolution of the line-integrated x-ray signals is used to determine the runaway radial transport coefficient in the central region of the plasma ( for r/a
- Published
- 1996
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26. Stationary magnetic shear reversal experiments in Tore Supra
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J.R. Ferron, R. Arslanbekov, G. T. Hoang, Yves Peysson, X. Litaudon, Ph. Bibet, F. Kazarian-Vibert, K Kupfer, D. Moreau, G. Rey, E. Joffrin, M. Goniche, and P. Froissard
- Subjects
Materials science ,Condensed matter physics ,Wave propagation ,Plasma ,Tore Supra ,Condensed Matter Physics ,Thermal diffusivity ,Magnetic field ,Geomagnetic reversal ,Nuclear Energy and Engineering ,Physics::Plasma Physics ,Atomic physics ,Absorption (electromagnetic radiation) ,Current density - Abstract
Stable and stationary states with hollow current density profiles have been achieved in Tore Supra with lower hybrid current drive (LHCD) during reduced toroidal magnetic field operation and in weak LH absorption regimes. For these plasma conditions, off-axis LH power deposition profiles are obtained in a reproducible manner when the internal LH caustics prevent central absorption of the waves. In the multipass LH wave propagation regime, the validity of the statistical treatment of stochastic wave diffusion is shown both theoretically and experimentally. When a large fraction of the plasma current (above 50%) is non-inductively sustained by the LH waves, the magnetic shear is reversed in the plasma core, i.e. inside a normalized plasma radius of the order of 0.4. The resulting hollow current density profiles have led to an enhancement of the total electron thermal energy content, up to a factor of 1.6 compared with L-mode discharges. The confinement improvement is attributed to a strong reduction of the electron thermal diffusivity in the central reversed shear region, nearly down to its neoclassical level.
- Published
- 1996
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27. Modelling of lower hybrid current drive and comparison with experimental results in JET
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C. Gormezano, F.G. Rimini, P. Froissard, F. X. Söldner, A. Ekedahl, Yu.F. Baranov, and M. Lennholm
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Physics ,Nuclear and High Energy Physics ,Jet (fluid) ,Wave propagation ,Scattering ,Electric field ,Bremsstrahlung ,Electron ,Current (fluid) ,Atomic physics ,Diffusion (business) ,Condensed Matter Physics ,Computational physics - Abstract
A new code has been developed and benchmarked with data from lower hybrid current drive (LHCD) experiments in JET. The model includes stochastic radial diffusion of electrons and scattering of lower hybrid (LH) waves. The code is validated by comparing calculated and experimental current drive efficiencies as well as calculated and measured hard X ray bremsstrahlung emission radial profiles. Reasonable agreement has been found for full LH current driven cases and LH plus moderate DC electric field
- Published
- 1996
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28. Review of neutral beam heating on JET for physics experiments and the production of high fusion performance plasmas
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R. König, N. Peacock, E. Martin, L. Lauro-Taroni, D.V. Bartlett, B. Ingram, G. Vlases, A. Sibley, C. Terella, C. Lowry, N. A. Gottardi, T. Elevant, G. Saibene, J. Christiansen, M. Baronian, A. Tesini, T. Raimondi, A. J. Bickley, J. How, H. van der Beken, A. Haigh, N. C. Hawkes, M. C. Ramos de Andrade, H. Morsi, G. Murphy, M. Botman, A. Dines, A. Gondhalekar, C. Gormezano, M. Irving, H. Brelen, M. Tabellini, B. Schunke, B.J.D. Tubbing, G. Sadler, P. R. Thomas, C. Gowers, P. E. Stott, G. Corrigan, S. Cooper, W. J. Brewerton, H. D. Falter, M. Keilhacker, A. Korotkov, V. Marchese, M. Cox, P. Breger, M. Nilsen, T. Szabo, M. L. Watkins, R. Claesen, C. J. Hancock, I. D. Young, S. Ali-Arshad, M. J. Watson, O. N. Jarvis, E. Bertolini, C. Walker, S. Clement, Y. Baranov, W. Bailey, G. Celentano, C. Froger, K. D. Lawson, D. Stork, D.F.H. Start, A. Cherubini, R. Monk, S. L. Dmitrenko, H. Jaeckel, S. Richards, C. A. Steed, L. G. Eriksson, S. F. Mills, S. J. Booth, P. G. Doyle, P. Meriguet, R. J. M. Pearce, H. Duquenoy, G. Radford, R. Prentice, F. Jensen, M. A. Pick, C. D. Challis, B. Alper, R. Wolf, J. Lingertat, F. Soldner, M. O'Mullane, N. Deliyanakis, P. Nielsen, A. C. Bell, R. Lasser, E. Deksnis, J. P. Coad, P. J. Harbour, E. M. Jones, T. Budo, F. Marcus, N. Davies, B. Balet, F.G. Rimini, M. Comiskey, T. Wade, P. Burton, T. Bonicelli, P. Gaze, K. Fullard, D. Martin, W. Zwingmann, T. Winkel, M. Ottaviani, P. Massmann, J. O'Rourke, D. Bond, P. Boucquey, P. Barabaschi, R. D. Gill, M. Cooke, B. Patel, W. Suverkroop, A. Kaye, D. Chiron, T. Businaro, D. Goodall, M.F. Stamp, G. B. Denne-Hinnov, R. Ostrom, A. Girard, L. Horton, F. Trevalion, C. Woodward, J. Ehrenberg, M. Johnson, A. Loarte, S. Puppin, R. Simoni, J. Jacquinot, A. Galetsas, W. Obert, M. Schmid, J. F. Junger, J. F. Jaeger, P. Andrew, L. Rossi, K. Borras, P. Smeulders, R. Reichle, A. Rolfe, J. Plancoulaine, P. Chuilon, T. T. C. Jones, R. Barnsley, A. Gibson, P. Card, N. Dolgetta, R. Rookes, M. Rapisarda, A. Colton, P. Schild, H. Buttgereit, M. von Hellermann, C. Perry, Henrik Bindslev, M. Garribba, F. Hurd, J. Mart, C. Sborchia, S. M. Scott, K. Blackler, A. Santagiustina, G. Bosia, C. Cottrell, I. Coffey, G. Newbert, S. Papastergiou, P. Butcher, L. Svensson, G. Vayakis, O. Da Costa, T. Hender, S. Weber, C. F. Maggi, V. V. Parail, P. Froissard, A. Taroni, A.E. Costley, J. P. Poffe, V.P. Bhatnagar, A. C. Maas, Y. Agarici, K. Thomsen, H. McBryan, Francesco Porcelli, H. Altmann, T. J. Wijnands, T. Brown, R. T. Ross, D. O'Brien, R. N. Litunovski, J. J. Davies, R. Russ, P. Kupschus, Annika Ekedahl, G. Magyar, G. Fishpool, H. Deesch, A. C. C. Sips, N. G. Kidd, C. Caldwell-Nichols, T. P. Hughes, M. Newmann, R. Sartori, S. Corti, S. K. Erents, T. Martin, R. Haange, A. M. Edwards, J.A. Dobbing, M. Gadeberg, G. Matthews, Laurie Porte, M. Wykes, D. Wilson, S. J. Davies, J. M. Adams, D. Ward, Wolfgang Kerner, L. Zannelli, J. G. Cordey, A. Tanga, P. Peacock, P. Bertoldi, H. Summers, L. Galbiati, W. J. Dickson, N. P. Hawkes, Michael Loughlin, David Campbell, D. Summers, P. Stangeby, D. Campling, J. L. Hemmerich, G. Benali, S. E. Dorling, J.A. Hoekzema, P. Haynes, J. L. Salanave, F. Junique, M. Salisbury, M. Brusati, J. Wesson, E. Oord, R. Giannella, M. Bures, J. Freiling, G. Janeschitz, M. Huart, E. Righi, G. Sanazzaro, P. J. Lomas, G. Deschamps, P. Stubberfield, M. Lennholm, E. Thompson, B. Macklin, P. J. Howarth, L. P. D. F. Jones, B. E. Keen, P. Noll, M. Brandon, R. Smith, P. Barker, F. Nave, P.D. Morgan, and P. Crawley
- Subjects
Physics ,Fusion ,Jet (fluid) ,Mechanical Engineering ,Nuclear engineering ,Plasma ,Neutral beam injection ,Ion ,Nuclear physics ,Nuclear Energy and Engineering ,Nuclear fusion ,General Materials Science ,Tritium ,Beam (structure) ,Civil and Structural Engineering - Abstract
The JET neutral beam injection system has proved to be both effective and reliable as a plasma heating device. The ion heating and plasma fuelling characteristics of the system are ideally suited to the production of high fusion performance plasmas while the flexibility in the choice of beam species (H, D, T, 3 He or 4 He) and the ability to inject into almost any JET plasma configuration allows a wide variety of related physics experiments to be carried out. The capability to inject (for the first time) tritium beams was essential to the successful execution of the first tritium experiments in which 1.7 MW of power from DT fusion reactions was generated.
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- 1995
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29. Overview of high performance H-modes in JET
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A. C. C. Sips, A.E. Costley, F. Hurd, G. Saibene, M. Salisbury, M. Brusati, C. Perry, P. J. Harbour, T. Martin, J. P. Poffe, Laurie Porte, H. van der Beken, N. C. Hawkes, J. Wesson, M. Bures, G. Janeschitz, M. Huart, A. Santagiustina, G. Bosia, H. Altmann, J. L. Salanave, A. Dines, N. G. Kidd, F. Junique, E. Righi, P. J. Lomas, P. G. Doyle, J. G. Cordey, G. Magyar, V. V. Parail, K. Thomsen, A. Gondhalekar, M. Irving, C. Gowers, R. Ostrom, C. Woodward, A. Galetsas, A. Loarte, P. Card, P. Trevalion, A. M. Edwards, T. P. Hughes, F. Jensen, M. Newman, C. Caldwell-Nichols, N. Peacock, P. Smeulders, A. Korotkov, A. Colton, P. Chuilon, T. T. C. Jones, F.G. Rimini, T. Winkel, P. Stubberfield, M. A. Pick, J.A. Hoekzema, T. Szabo, J. M. Adams, R. Prentice, Wolfgang Kerner, L. Zannelli, M. Rapisarda, D.F.H. Start, L. G. Eriksson, P. Schild, M. Wykes, D. Wilson, S. J. Davies, A. Sibley, P. Haynes, B. Alper, R. Wolf, T. Elevant, R. T. Ross, J. O'Rourke, E. Thompson, C. J. Hancock, R. Haange, P. E. Stott, A. Tesini, B. Macklin, M. Baronian, W. J. Brewerton, M.F. Stamp, L. P. D. F. Jones, A. C. Maas, B. E. Keen, A. Taroni, H. Morsi, G. Murphy, H. D. Falter, M. Keilhacker, I. D. Young, M. von Hellermann, A. Girard, A. Haigh, M. Cooke, A. Cherubini, Henrik Bindslev, D. Goodall, L. Horton, S. K. Erents, J.A. Dobbing, M. Gadeberg, E. Deksnis, G. Matthews, M. Comiskey, T. Wade, F. Marcus, M. Schmid, P. Burton, M. Garribba, G. Newbert, P. Barabaschi, A. Peacock, V. Marchese, C. Froger, K. D. Lawson, P. Noll, M. Brandon, G. Sadler, P. R. Thomas, C. F. Maggi, W. Bailey, D. Ward, K. Blackler, A. Rolfe, T. J. Wijnands, R. Barnsley, G. Celentano, R. Russ, Annika Ekedahl, G. Vayakis, T. Bonicelli, P. Froissard, C. Walker, J. Jacquinot, J. Plancoulaine, P. Kupschus, N. Dolgetta, Y. Agarici, D. Summers, M. Ottaviani, H. Brelen, S. Ali-Arshad, C. Sborchia, R. Claesen, C. A. Steed, S. F. Mills, A. Gibson, R. Smith, B. Schunke, B.J.D. Tubbing, J. Mart, H. McBryan, L. Svensson, J. J. Davis, S. M. Scott, R. J. M. Pearce, J. P. Coad, F. Soldner, T. Budd, P. Stangeby, E. M. Jones, V.P. Bhatnagar, C. D. Challis, R. Rookes, D. Campling, I. Coffey, W. Zwingmann, A. C. Bell, E. Oord, D. O'Brien, P. Gaze, N. Davies, D. Bond, David Campbell, P. Barker, F. Nave, G. B. Denne-Hinnov, S. Papastergiou, R. Monk, S. L. Dmitrenko, B. Balet, P. Butcher, L. Rossi, K. Borras, O. Da Costa, R. Giannella, P. Massmann, R. D. Gill, R. Sartori, J. Lingertat, S. Weber, R. N. Litunovski, H. Buttgereit, J. Ehrenberg, B. Patel, R. Lasser, N. A. Gottardi, A. Kaye, T. Brown, J. Christiansen, T. Businaro, L. Lauro-Taroni, C. Gormezano, O. N. Jarvis, S. Clement, A. J. Bickley, J. Freiling, D.V. Bartlett, D. Chiron, M. Botman, B. Ingram, C. Terella, C. Lowry, W. Obert, M. Tabellini, S. Corti, S. Cooper, P. Bertoldi, E. Bertolini, H. Summers, P.D. Morgan, P. Crawley, R. Reichle, Francesco Porcelli, G. Sanazzaro, G. Corrigan, T. Raimondi, G. Deschamps, M. J. Watson, M. C. Ramos de Andrade, G. Fishpool, H. Deesch, J. L. Hemmerich, G. Benali, Y. Baranov, H. Jaeckel, S. E. Dorling, G. Radford, S. J. Booth, J. F. Junger, H. Duquenoy, M. Lennholm, L. Galbiati, W. J. Dickson, N. P. Hawkes, R. Simonini, Michael Loughlin, T. Hender, M. Cox, P. Breger, W. Suverkropp, M. Nilsen, M. L. Watkins, S. Puppin, D. Stork, S. Richards, P. Nielsen, P. Boucquey, G.A. Cottrell, A. Tanga, P. J. Howarth, K. Fullard, D. Martin, M. Johnson, J. F. Jaeger, P. Andrew, P. Meriguet, Ralf König, M. O'Mullane, N. Deliyanakis, E. Martin, G. Vlases, and J. How
- Subjects
Physics ,Nuclear Energy and Engineering ,Diamagnetism ,Plasma ,Atomic physics ,Condensed Matter Physics ,Phenomenology (particle physics) ,Scaling ,Ion - Abstract
An account is given of the high performance plasmas established by development of the H-mode regime in JET in the experimental campaigns up to 1992. High performance in this case is measured in terms of the confinement enhancement achieved over the L-mode scaling as measured using the plasma diamagnetism. Three JET H-mode regimes have achieved enhancement factors (H G DIA ) over Goldston L-mode scaling of 2.5 < H G DIA < 4.0. These are the Pellet Enhanced Performance (PEP) H-MODE, the high bootstrap fraction (high β POL ) H-mode and the Hot Ion (HI) H-mode. The phenomenology of these three regimes is reviewed and contrasts and common threads are elucidated
- Published
- 1994
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30. Determination of the suprathermal electron distribution function during lower hybrid current drive in JET
- Author
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Annika Ekedahl, D.V. Bartlett, A. Airoldi, M. Brusati, R. P. da Silva, P. Froissard, G. Ramponi, and Yves Peysson
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Physics ,Nuclear and High Energy Physics ,Jet (fluid) ,Astrophysics::High Energy Astrophysical Phenomena ,Cyclotron ,Electron ,__ ,Condensed Matter Physics ,Lower hybrid oscillation ,law.invention ,Magnetic field ,Distribution function ,law ,Plasma diagnostics ,Current (fluid) ,Atomic physics - Abstract
The information provided by different diagnostics has been combined in order to characterize the fast electron distribution function in lower hybrid current drive experiments on JET. In particular, X ray and electron cyclotron emission data are complementary in defining the fast electron energy contents in the directions parallel and perpendicular to the magnetic field. A numerical analysis chain has been developed which identifies the main moments of the distribution function of the current carrying fast electrons and allows simulations based on these moments to be compared with X ray emission, electron cyclotron emission and magnetic data. The method of analysis and the associated diagnostics are described, and results are presented which have been obtained during the lower hybrid current drive campaign on JET.
- Published
- 1994
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31. Study of radiation scattering in the hard X ray energy range by a tokamak inner wall
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C. Pocheau, P. Froissard, and Y. Peysson
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Physics ,Nuclear and High Energy Physics ,Range (particle radiation) ,Tokamak ,Scattering ,Astrophysics::High Energy Astrophysical Phenomena ,Monte Carlo method ,Compton scattering ,X-ray ,Plasma ,Condensed Matter Physics ,law.invention ,Physics::Plasma Physics ,law ,Plasma diagnostics ,Atomic physics - Abstract
A detailed investigation is presented of the X rays that emerge from a tokamak inner wall after a random walk in which Compton scattering is the dominant process. The ability to measure accurately plasma emission above 50 keV along lines of sight facing the inner wall is assessed using a Monte Carlo code, the standard solution for radiation transport in a bounded medium. For peaked emission profiles, the scattered contribution can be large compared with the direct plasma emission for lines of sight probing the border of the plasma. Good quantitative agreement is found between the predicted and the measured hard X ray data for this case
- Published
- 1993
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32. The lower hybrid current drive system for ITER: progress on the route of the new launchers
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C. Gormezano, P. Bibet, J.G. Wegrowe, P. Schild, R. Walton, L. Doceul, G. Rey, Frej Wasastjerna, P. Froissard, A. Kaye, F. X. Söldner, F. Kazarian, H. Panissie, G. Tonon, C. Portafaix, and M. Goniche
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Coupling ,Incident power density ,Engineering ,Long pulse ,business.industry ,Electrical engineering ,Current (fluid) ,Converters ,business ,Power (physics) - Abstract
The design of a Lower Hybrid Waves (LHW) system for ITER EDA is based on the robust concept of the passive active multijunction (PAM) which associates a good coupling of the slow wave with an efficient cooling of the grill mouth. The PAM is fed by an oversized section (hyperguide) exited by several mode converters which ensure the poloidal sharing of the RP power. Specific components, such as the mode converter and hyperguide have now been successfully tested at high power and long pulse duration (up to 1000 s on test bed). The 50 MW required to cover the envisioned scenarios, can be coupled by two launchers located in two ports of the machine. The working incident power density is 23 MW/m/sup 2/ which is routinely achieved in present experiments at 3.7 GHz.
- Published
- 2002
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33. Tore supra lower hybrid test bed: Description and results
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F. Kazarian, J. Clary, P. Froissard, L. Tanaskovic, M. Goniche, P. Bibet, S. Dutheil, G. Rey, S. Poli, and B. Beaumont
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Steady state (electronics) ,Computer science ,Electronic engineering ,Radio frequency ,Tore Supra ,Simulation - Abstract
The new Tore Supra objective is to produce steady state operation up to 1000 seconds. The Lower Hybrid test bed of Tore Supra has been developed to validate Radio Frequency (RF) concepts compatible with this objective. The overall layout and specification is presented together with operation modes and an example of test campaign.
- Published
- 1999
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34. Preliminary design of an antenna made of passive active multijunctions for LHCD for test on FTU
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F. Kazarian, A. Marra, M. Roccon, P. Froissard, Ph. Bibet, A. A. Tuccillo, and Francesco Mirizzi
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Engineering ,Microwave amplifiers ,business.industry ,Frascati Tokamak Upgrade ,Electrical equipment ,Nuclear engineering ,Time schedule ,Electrical engineering ,Iter tokamak ,Antenna (radio) ,business ,Electronic equipment - Abstract
To test on the Frascati Tokamak Upgrade (FTU) the concept of Passive Active Multijunction (PAM) proposed for Lower Hybrid Current Drive (LHCD) on ITER, studies and design have been made in collaboration between ENEA and CEA. The main properties of this launcher have been analyzed. A description of the antenna and the time schedule toward the experiment is given.
- Published
- 1999
- Full Text
- View/download PDF
35. High ICRF power in Tore Supra
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G. Martin, F. Kazarian, S. Brémond, I. Monakhov, B. Beaumount, L. Colas, L. G. Eriksson, P. Froissard, A. Bécoulet, V. Basiuk, F. Nguyen, L. Ladurelle, P. Bibet, and V. Bergeaud
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law ,Chemistry ,Cyclotron resonance ,Electron temperature ,Electron ,Plasma ,Tore Supra ,Atomic physics ,Faraday cage ,Electromagnetic radiation ,law.invention ,Bootstrap current - Abstract
The aim of the ICRF system of Tore Supra is to provide high power to the plasma and to sustain long pulse operation. Good results have been obtained at high power in minority heating at 57 MHz on He(H) plasmas: 10 MW of ICRH alone have been coupled and 12 MW of combined heating (minority ICRH+LH) have been sustained for 2 s. The new lateral bumpers installed on one antenna are very satisfactory for power handling. ICRH experiments in 3He minority (42 MHz, B0=3.7 T) have also been carried out. In particular, 6 MW have been coupled in D plasma. The influence of the 3He concentration has been studied. There is experimental evidence of electron heating with mode conversion at high concentration (10–20%) of 3He. Without 3He, 3 MW have been coupled in FWEH on similar D target plasma. A scan in plasma current has been performed (1.5→1.1 MA). The bootstrap current is estimated. Some edge phenomena are observed: heating of the septum and other parts of the Faraday screen occurs when a cyclotron resonance layer is ...
- Published
- 1999
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36. Flexible N// Launcher for Lower Hybrid Wave injection in the first operation phase of ITER
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S. Berio, J.‐G. Wégrowe, G. Tonon, X. Litaudon, Gt. Hoang, Ph. Bibet, M. Tareb, F. Kazarian-Vibert, G. Rey, M. Goniche, and P. Froissard
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Physics ,Antenna design ,Nuclear engineering ,Frame (networking) ,Phase (waves) ,Plasma ,Reflection coefficient ,Lower hybrid oscillation ,Power (physics) - Abstract
A first conceptual antenna design based on the passive-active multijunction (PAM), was proposed in 93 in the frame of an ITER-TASK [ 1 ]. The present work accounts for the dimensional change of the ITER ports and also proposes a new arrangement of the hyperguides feeding the PAM to allow the flexibility of the N // spectrum which is desirable to cover the variety of envisioned scenarios. 35 to 55 MW of LH power could be injected in 1 port of ITER depending on the actual plasma reflection coefficient.
- Published
- 1997
- Full Text
- View/download PDF
37. Long pulse performance with the LHCD system on Tore-Supra
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T. Hoang, P. Bibet, Y. Peysson, X. Litaudon, M. Goniche, C. Portafaix, G. Rey, G. Martin, D. Guilhem, F. Kazarian-Vibert, J.Y. Journeaux, P. Froissard, and T. Wijnands
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Materials science ,Steady state (electronics) ,Control theory ,Plasma parameters ,Hybrid system ,Nuclear engineering ,Plasma ,Tore Supra ,Electromagnetic radiation ,Power density ,Power (physics) - Abstract
High power and energy performance have been achieved by the Tore-Supra Lower Hybrid system during the last experimental campaign. Plasma pulses up to 120 s in steady state conditions with a power density up to 24 MW/m2 were obtained. A combination of feedback controls of the LH and plasma parameters have also been used to investigate long pulse and current profile control operating scenarios.
- Published
- 1997
- Full Text
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38. High performance results with the LHCD system on Tore-Supra and new Launcher design for quasi continuous operation
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M. Goniche, F. Surle, G. Agarici, P. Hertout, S. Berio, F. Kazarian-Vibert, C. Deck, Yves Peysson, X. Litaudon, G. Rey, P. Froissard, D. Guilhem, G. Martin, J.Y. Journeaux, C. Portafaix, J.‐G. Wégrowe, T. Hoang, L. Garampon, Ph. Bibet, M. Mattioli, G. Tonon, and M. Tareb
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Coupling ,Physics ,Steady state ,Continuous operation ,Nuclear engineering ,Plasma ,Tore Supra ,Converters ,Power density ,Power (physics) - Abstract
High power and energy performance have been achieved by the Tore-Supra LH system during the last experimental campaign. Far distance coupling as well as plasma pulses up to 120 s in steady state conditions with a power density of 24 MW/m2 were obtained. A new launcher, made with RF components such as mode converters has been designed in order to extend the present TS performance towards quasi continuous operations.
- Published
- 1997
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39. New launchers for Tore Supra LHCD
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G. Rey, M. Goniche, P. Froissard, L. Cogneau-Garampon, G. Tonon, P. Hertout, R. Mitteau, G. Agarici, F. Kazarian-Vibert, Ph. Bibet, C. Portafaix, and C. Deck
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Materials science ,business.industry ,Electrical engineering ,Aerospace engineering ,Tore Supra ,Waveguide array ,business ,Plasma current - Abstract
In order to increase the reliability of the launchers for LHCD on Tore Supra, to drive the main part of the plasma current for long pulses (1000 s), new antennae have been studied. Their front tip can be of 2 types: the first one using multijunctions, the second one using active-passive waveguide array to test this new concept for ITER. After a brief description of their design, results of the main studies and tests in order to realize them are given.
- Published
- 1997
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40. Quasi-optical LHW launcher pre-design study for Tore-Supra, modelizations
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P. Garin, Ph. Bibet, G. Rey, J.P. Crenn, Gilles Berger-By, M. Pain, G. Tonon, J.‐G. Wégrowe, H. Kuus, G. Agarici, and P. Froissard
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Diffraction ,Power transmission ,Engineering ,Mathematical model ,Robustness (computer science) ,business.industry ,Electric field ,Electrical equipment ,Design study ,Electronic engineering ,Tore Supra ,Aerospace engineering ,business - Abstract
The ‘Quasi‐Optical Grill’ (QOG) has been proposed to increase the robustness and the simplicity of a Lower‐Hybrid Waves antenna for reactor application [1]. To compare the performance of a QOG antenna with that of conventional scheme, an experiment was envisaged in TORE‐SUPRA in cooperation with the ENEA. Encouraging modelling results [2] lead to conduct a pre‐design study [3]. The main results of this study are presented in this paper. In particular, an analysis of the practical system has shown a limitation of the injected power due to either the appearance of large electric fields in the resonant structure, or to an increased reflexion linked with departures from ideal conditions. This analysis is based on the results of the diffraction code [2] developed by the ENEA complemented by a simple ‘Fabry‐Perot’ model. A further optimization is thus needed and is being undertaken to assess the future of the concept.
- Published
- 1996
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41. LHCD OPERATIONS IN JET AND DEVELOPMENTS FOR FUSION APPLICATIONS
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A. Kaye, P. Schild, Y. Baranov, P. Finburg, T. McCarthy, J. Plancoulaine, P. Haydon, F.G. Rimini, J.A. Dobbing, A. Ekedahl, G. Agarici, B. Fischer, R. Page, C. Gormezano, P. Paling, M. Brandon, G. Platt, M. Lennholm, P. Froissard, M. Brusati, F. X. Söldner, and D. Pledge
- Subjects
Coupling ,Physics ,Fusion ,Jet (fluid) ,business.industry ,Active feedback ,Plasma ,Aerospace engineering ,business ,Power (physics) - Abstract
The full Lower Hybrid Current Drive (LHCD) launcher in JET has been completed and installed. Up to 6 MW has been coupled to the plasma and 2 MA full current drive was achieved with 4 MW of LH power. Coupling is improved by using active feedback control on the launcher and plasma position. A new launcher design concept, called Hyperguide, has been developed and tested successfully in JET to replace eventually conventional launchers using multijunctions for next step machines like ITER.
- Published
- 1995
- Full Text
- View/download PDF
42. Tellurium halide IR fibers for remote spectroscopy
- Author
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Karine Le Foulgoc, D. Picque, Hongli Ma, Jacques Lucas, Xhang Hua Zhang, Jean Heuze, Chantal Blanchetiere, P. Colardelle, P. Froissard, and G. Corrieu
- Subjects
Optical fiber ,Materials science ,Spectrometer ,Analytical chemistry ,Infrared spectroscopy ,chemistry.chemical_element ,Halide ,law.invention ,Devitrification ,chemistry ,law ,Fourier transform infrared spectroscopy ,Tellurium ,Spectroscopy - Abstract
The new family of IR transmitting glasses, the TeX glasses, based on the association of tellurium and halide (Cl, Br, or I) are characterized by a wide optical window extending from 2 to 18 micrometers and a strong stability towards devitrification. Optical fibers drawn from these glasses exhibit low losses in the 7 - 10 micrometers range (less than 1 dB/m for single index fibers, 1 - 2 dB/m for fibers having a core-clad structure). The TeX glass fibers have been used in a remote analysis set-up which is mainly composed of a FTIR spectrometer coupled with a HgCdTe detector. This prototype system permits qualitative and quantitative analysis in a wide wavelength region lying from 3 to 13 micrometers , covering the fundamental absorption of more organic species. The evolution of a lactic and an alcoholic fermentation has been monitored by means of this set-up.
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- 1994
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43. First results with the modified JET
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S. Clement, P. Nielsen, D. V. Bartlett, R. Goulding, M. Bures, P. Boucquey, R. Claesen, M. Cox, P. Breger, W. Suverkropp, M. L. Watkins, S. Richards, C. J. Hancock, I. D. Young, P. Card, T. Raimondi, H. Jaeckl, R. Ostrom, J. Mart, Henrik Bindslev, E. van der Goot, C. Woodward, D. Summers, R. König, B. Schunke, T. Hender, E. Thompson, F. Sartori, F. Cecil, P. Chuilon, T. T. C. Jones, S. Milani, G. Saibene, R. Prentice, B. Macklin, L. Svensson, J. Lingertat, P. Smeulders, R. L. Shaw, N. Watkins, R. J. M. Pearce, H. van der Beken, N. C. Hawkes, J. Jacquinot, P. Stubberfield, N. G. Kidd, T. P. Hughes, T. Elevant, R. Monk, W. Zwingmann, J.A. Hoekzema, S. Papastergiou, G. Vlases, R. Lasser, B. Fischer, S. L. Dmitrenko, C. D. Challis, L. Lauro-Taroni, F. Hurd, R. T. Ross, R. Cusack, D. Wilson, H. Brelen, David Campbell, A. C. Maas, G. Cottrell, E. Bertolini, J. How, J. Plancoulaine, R. Russ, O. Da Costa, K. Lawson, J. P. Poffe, N. Peacock, L. Porte, L. Galbiati, S. Ali-Arshad, B. Ingram, B. Alper, R. Wolf, K. Fullard, E. Oord, H. Altmann, A. M. Edwards, C. Terella, C. Lowry, D. Martin, E. Deksnis, A. Rossi, D. O'Brien, S. J. Davies, J. J. Davis, F. Marcus, R. Giannella, C. Walker, V. Bhatnagar, C. A. Steed, A. Gibson, F. Jensen, A. Ekedahl, M. Baronian, J. M. Adams, M. A. Pick, E. M. Jones, N. P. Hawkes, G. D'Antona, P. Schild, L. Horton, J. Freiling, D. Bond, G. Sadler, Wolfgang Kerner, D. Stork, N. Davies, S. K. Erents, R. Simonini, Michael Loughlin, M. Stamp, M. Schmid, P. J. Harbour, L. Zannelli, M. Schaffer, R. D. Gill, F. Nguyen, M. Gadeberg, A. Tanga, A. Haigh, B. Balet, F. Junique, G. Matthews, M. Johnson, G. Sanazzaro, P. R. Thomas, T. Budd, J. L. Hemmerich, G. Benali, A. Peacock, S. E. Dorling, G. McCormick, J. Dobbing, J. F. Jaeger, P. Andrew, P. Froissard, C. Perry, A. Santagiustina, M. von Hellermann, W. Bailey, D. Ward, C. Caldwell-Nichols, B. Patel, G. Celentano, T. Wade, V. V. Parail, T. Businaro, E. Lazzaro, A. C. Bell, G. Vayakis, G. Fishpool, H. Deesch, F. Rimini, S. Colombi, S. Puppin, B. Tubbing, W. Obert, A. C. C. Sips, F. Soldner, J. Christiansen, A. Sibley, N. Zornig, A. Rookes, M. Salisbury, A. Kaye, D. Chiron, N. A. Gottardi, J. Wesson, S. Sharapov, T. Martin, S. Weber, P. Lomas, D. Campling, P. Lamalle, A. Rolfe, M. Cooke, D. Goodall, A. J. Bickley, N. Deliyanakis, P. Morgan, A. Tesini, B. Fechner, I. Hutchinson, M. Garribba, R. Reichle, F. Porcelli, R. Barnsley, G. Newbert, G. Murphy, M. Tabellini, P. Stangeby, M. Huart, S. Cooper, E. Righi, L. Rossi, K. Borras, L. G. Eriksson, C. F. Maggi, C. Froger, J. G. Cordey, N. Dolgetta, H. Buttgereit, O. Pogutse, Y. Agarici, S. M. Scott, S. Ishida, D. F. Start, P. E. Stott, A. Meigs, W. J. Brewerton, H. D. Falter, M. Keilhacker, J. P. Coad, V. Marchese, A. Dines, A. Gondhalekar, M. Irving, C. Gowers, H. Guo, K. Thomsen, C. Laviron, C. Gormezano, A. Taroni, A. Howman, P. Kupschus, T. Szabo, M. Ottaviani, P. Burton, P. Ageladarakis, O. N. Jarvis, J. Ehrenberg, P. Savrukhin, T. Bonicelli, M. J. Watson, Y. Baranov, H. Duquenoy, T. Brown, Ambrogio Fasoli, P. G. Doyle, P. Crawley, G. Radford, L. P. D. F. Jones, B. E. Keen, P. Noll, M. Brandon, U. Gerstel, T. Winkel, R. Smith, J. F. Junger, E. Lyadina, P. Barker, F. Nave, P. J. Howarth, M. Lennholm, K. Blackler, H. McBryan, C. Grisolia, R. Sartori, and H. Summers
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Physics ,Jet (fluid) ,Nuclear engineering ,Divertor ,Plasma ,Condensed Matter Physics ,Power (physics) ,Nuclear physics ,Nuclear Energy and Engineering ,___ ,Power handling ,Electron heating ,Current (fluid) ,Scaling - Abstract
JET was extensively modified in the 1992/93 shutdown. The new pumped divertor and many new systems were brought into operation early in 1994. Operations have progressed to 4MA plasma current and, with substantial additional heating, H-mode confinement results confirm the expected scaling. The high power handling capability of the pumped divertor with sweeping is estimated at 20MW for 20s. H-mode plasmas have large Type I ELMs. With lower hybrid heating alone, 2MA full current drive has been achieved with good efficiency, with ICRF power, effective heating and direct electron heating have been demonstrated.
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- 1994
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44. Recent Results from the Lower Hybrid Current Drive Experiment on JET
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M. Brusati, M. Lennholm, P. Froissard, M. Pain, D. Start, C. Gormezano, P. Schild, J. Jacquinot, A. Ekedahl, M. C. R. de Andrade, P. J. Lomas, and F.G. Rimini
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Jet (fluid) ,Chemistry ,Bremsstrahlung ,Limiter ,Flux ,Electron temperature ,Plasma ,Atomic physics ,Current (fluid) ,Ohmic contact - Abstract
The JET Lower Hybrid Current Drive (LHCD) prototype system has been in operation in the early part of the 1991 experimental campaign with generated power up to 2.7 MW at 3.7 GHz and coupled power to the plasma up to 2.4 MW. Experiments have focused so far on the determination of the performance limits of the system, both in terms of long pulse operation and of replacement of the ohmically driven plasma current. Further experiments have also been carried out to confirm early findings of central electron heating induced by LH when applied in combination with Ion Cyclotron Resonance Heating (ICRH). Long pulse operation has been obtained on JET with 2 MA, 1.9 T limiter plasmas with 1 minute current flat top where 40% of the required flux is provided by the combined application of the LH and ICRF systems. Full replacement of the inductive current has been observed in 1 MA, 3.4 T ohmic plasmas with 1.9 MW of LH power, with current drive efficiencies up to 〈ne〉 Icd/Plh∼0.35 1020 m −2 A/W with volume averaged ele...
- Published
- 1992
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45. Fate and forms of Cu in a reservoir ecosystem following copper sulfate treatment (Saint Germain les Belles, France)
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Van Hullebusch, E., Chatenet, P., Deluchat, V., Chazal, P. M., Froissard, D., Lens, P. N.L., Baudu, M., Van Hullebusch, E., Chatenet, P., Deluchat, V., Chazal, P. M., Froissard, D., Lens, P. N.L., and Baudu, M.
- Abstract
Copper sulfate (CuSO4) addition to freshwater for phytoplankton control has been practiced for decades, and remains the most effective algicidal treatment for numerous managed water bodies. A reservoir in the centre of France was the site for an investigation of copper distribution in aquatic systems after a copper sulfate treatment Results of copper monitoring showed a rapid conversion of dissolved Cu to particulate forms, with significant accumulation in the sediments/83% of total copper added). Total sediment Cu content increasedfrom approximately 37.7 to 45.4 μg.g-1dry weight after the first treatment. Sequential extraction suggested that a significanl portion of the sediment-borne Cu was associated with the organic fraction which may release Cu to the water column, although significant release would occur only under extreme changes in water chemistry. Based upon measured Cu concentrations, flows at the down-stream water, and known mass applied during treatment, mass balance calculations indicated that approximately 17% of the Cu was exported from the reservoir over a 70 day period following a 196 μg.L-lCu2+(as CuSO4, 5 H2O) treatment. The largest amount of copper was probably adsorbed on downstream sediment or lost in running water, Copper bioaccumulation by a moss, Fontinalis antipyretica, in the down-stream water showed that it was possible to distinguish between a treated and an untreated area. The impact of copper treatment in the down-stream reservoir could be followed using mosses. The bioaccumulation data further showed that there is a distance effect which could be exploited to determine potential copper impact on receiving water bodies. Thirty days after copper sulfate addition, Fontinalis still indicated copper exposure.
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- 2003
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46. Scavenger Receptor BI Boosts Hepatocyte Permissiveness to Plasmodium Infection.
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Yalaoui, Samir, Huby, Thierry, Franetich, Jean-François, Gego, Audrey, Rametti, Armelle, Moreau, Martine, Collet, Xavier, Siau, Anthony, van Gemert, Geert-Jan, Sauerwein, Robert W., Luty, Adrian J.F., Vaillant, Jean-Christophe, Hannoun, Laurent, Chapman, John, Mazier, Dominique, and Froissard, Patrick
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PLASMODIUM falciparum genetics ,MALARIA prevention ,LOW density lipoproteins ,LIVER cells ,CELL receptors ,PATHOGENIC microorganisms - Abstract
Summary: Infection of hepatocytes by Plasmodium falciparum sporozoites requires the host tetraspanin CD81. CD81 is also predicted to be a coreceptor, along with scavenger receptor BI (SR-BI), for hepatitis C virus. Using SR-BI-knockout, SR-BI-hypomorphic and SR-BI-transgenic primary hepatocytes, as well as specific SR-BI-blocking antibodies, we demonstrate that SR-BI significantly boosts hepatocyte permissiveness to P. falciparum, P. yoelii, and P. berghei entry and promotes parasite development. We show that SR-BI, but not the low-density lipoprotein receptor, acts as a major cholesterol provider that enhances Plasmodium infection. SR-BI regulates the organization of CD81 at the plasma membrane, mediating an arrangement that is highly permissive to penetration by sporozoites. Concomitantly, SR-BI upregulates the expression of the liver fatty-acid carrier L-FABP, a protein implicated in Plasmodium liver-stage maturation. These findings establish the mechanistic basis of the CD81-dependent Plasmodium sporozoite invasion pathway. [Copyright &y& Elsevier]
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- 2008
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47. Multigene Family Encoding 3',5'-Cyclic-GMP-Dependent Protein Kinases in Paramecium tetraureliaCells
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Kissmehl, Roland, Kru¨ger, Tim P., Treptau, Tilman, Froissard, Marine, and Plattner, Helmut
- Abstract
ABSTRACTIn the ciliate Paramecium tetraurelia, 3',5'-cyclic GMP (cGMP) is one of the second messengers involved in several signal transduction pathways. The enzymes for its production and degradation are well established for these cells, whereas less is known about the potential effector proteins. On the basis of a current Parameciumgenome project, we have identified a multigene family with at least 35 members, all of which encode cGMP-dependent protein kinases (PKGs). They can be classified into 16 subfamilies with several members each. Two of the genes, PKG1-1and PKG2-1, were analyzed in more detail after molecular cloning. They encode monomeric enzymes of 770 and 819 amino acids, respectively, whose overall domain organization resembles that in higher eukaryotes. The enzymes contain a regulatory domain of two tandem cyclic nucleotide-binding sites flanked by an amino-terminal region for intracellular localization and a catalytic domain with highly conserved regions for ATP binding and catalysis. However, some ParameciumPKGs show a different structure. In Western blots, PKGs are detected both as cytosolic and as structure-bound forms. Immunofluorescence labeling shows enrichment in the cell cortex, notably around the dense-core secretory vesicles (trichocysts), as well as in cilia. Immunogold electron microscopy analysis reveals consistent labeling of ciliary membranes, of the membrane complex composed of cell membrane and cortical Ca2+stores, and of regions adjacent to ciliary basal bodies, trichocysts, and trafficking vesicles. Since PKGs (re)phosphorylate the exocytosis-sensitive phosphoprotein pp63/pf upon stimulation, the role of PKGs during stimulated exocytosis is discussed, in addition to a role in ciliary beat regulation.
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- 2006
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48. Antagonistic Roles of ESCRT and Vps Class C/HOPS Complexes in the Recycling of Yeast Membrane Proteins
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Bugnicourt, Amandine, Froissard, Marine, Sereti, Kostianna, Ulrich, Helle D., Haguenauer-Tsapis, Rosine, and Galan, Jean-Marc
- Abstract
In Saccharomyces cerevisiae, deficiencies in the ESCRT machinery trigger the mistargeting of endocytic and biosynthetic ubiquitinated cargoes to the limiting membrane of the vacuole. Surprisingly, impairment of this machinery also leads to the accumulation of various receptors and transporters at the plasma membrane in both yeast and higher eukaryotes. Using the well-characterized yeast endocytic cargo uracil permease (Fur4p), we show here that the apparent stabilization of the permease at the plasma membrane in ESCRT mutants results from an efficient recycling of the protein. Whereas several proteins as well as internalized dyes are known to be recycled in yeast, little is known about the machinery and molecular mechanisms involved. The SNARE protein Snc1p is the only cargo for which the recycling pathway is well characterized. Unlike Snc1p, endocytosed Fur4p did not pass through the Golgi apparatus en route to the plasma membrane. Although ubiquitination of Fur4p is required for its internalization, deubiquitination is not required for its recycling. In an attempt to identify actors in this new recycling pathway, we found an unexpected phenotype associated with loss of function of the Vps class C complex: cells defective for this complex are impaired for recycling of Fur4p, Snc1p, and the lipophilic dye FM4-64. Genetic analyses indicated that these phenotypes were due to the functioning of the Vps class C complex in trafficking both to and from the late endosomal compartment.
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- 2004
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49. Antagonistic roles of ESCRT and Vps class C/HOPS complexes in the recycling of yeast membrane proteins.
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Amandine, Bugnicourt, Marine, Froissard, Kostianna, Sereti, D, Ulrich Helle, Rosine, Haguenauer-Tsapis, and Jean-Marc, Galan
- Abstract
In Saccharomyces cerevisiae, deficiencies in the ESCRT machinery trigger the mistargeting of endocytic and biosynthetic ubiquitinated cargoes to the limiting membrane of the vacuole. Surprisingly, impairment of this machinery also leads to the accumulation of various receptors and transporters at the plasma membrane in both yeast and higher eukaryotes. Using the well-characterized yeast endocytic cargo uracil permease (Fur4p), we show here that the apparent stabilization of the permease at the plasma membrane in ESCRT mutants results from an efficient recycling of the protein. Whereas several proteins as well as internalized dyes are known to be recycled in yeast, little is known about the machinery and molecular mechanisms involved. The SNARE protein Snc1p is the only cargo for which the recycling pathway is well characterized. Unlike Snc1p, endocytosed Fur4p did not pass through the Golgi apparatus en route to the plasma membrane. Although ubiquitination of Fur4p is required for its internalization, deubiquitination is not required for its recycling. In an attempt to identify actors in this new recycling pathway, we found an unexpected phenotype associated with loss of function of the Vps class C complex: cells defective for this complex are impaired for recycling of Fur4p, Snc1p, and the lipophilic dye FM4-64. Genetic analyses indicated that these phenotypes were due to the functioning of the Vps class C complex in trafficking both to and from the late endosomal compartment.
- Published
- 2004
50. NSF regulates membrane traffic along multiple pathways in Paramecium.
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Roland, Kissmehl, Marine, Froissard, Helmut, Plattner, Massoud, Momayezi, and Jean, Cohen
- Abstract
N-ethylmaleimide (NEM)-sensitive factor (NSF), a regulator of soluble NSF attachment protein receptors (SNAREs), is required for vesicular transport in many eukaryotic cells. In the ciliated protozoon Paramecium, complex but well-defined transport routes exist, constitutive and regulated exocytosis, endocytosis, phagocytosis and a fluid excretory pathway through contractile vacuoles, that can all be studied independently at the whole cell level. To unravel the role of NSF and of the SNARE machinery in this complex traffic, we looked for NSF genes in Paramecium, starting from a partial sequence found in a pilot random sequencing project. We found two very similar genes, PtNSF1 and PtNSF2, which both seem to be expressed. Peptide-specific antibodies (Abs) recognize PtNSF as a 84 kDa band. PtNSF gene silencing results in decreasing phagocytotic activity, while stimulated exocytosis of dense core-vesicles (trichocysts), once firmly attached at the cell membrane, persists. Ultrastructural analysis of silenced cells shows deformation or disappearance of structures involved in membrane traffic. Aggregates of numerous small, smooth vesicles intermingled with branches of ER occur in the cytoplasm and are most intensely labeled with anti-NSF Ab-gold. Furthermore, elongated vesicles of approximately 30 nm diameter can be seen attached at cortical calcium storage compartments, the alveolar sacs, whose unknown biogenesis may thus be revealed. Involvement of PtNSF in some low frequency fusion events was visualized in non-silenced cells by immuno-fluorescence, after cautious permeabilization in the presence of ATP-gamma-S and NEM. Our data document that PtNSF is involved in distinct pathways of vesicle traffic in Paramecium and that actual sensitivity to silencing is widely different, apparently dependent on the turnover of membrane-to-membrane attachment formation.
- Published
- 2002
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