1. Immunoregulatory effect of mesenchymal stem cell via mitochondria signaling pathways in allergic asthma
- Author
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Seyyed Shamsadin Athari, Minmin Huang, and Entezar Mehrabi Nasab
- Subjects
OPA1, Mitochondrial dynamin like GTPase ,Allergy ,AHR, Airway hyperresponsiveness ,IT, Intratrachea administration ,MFN, Mitofusin ,Cytb, Cytochrome b ,MSC, mesenchymal stem cell ,Mitochondrion ,Ig, Immunoglobulin ,PGE2, Prostaglandin E2 ,IP, Intraperitoneal injection ,MSC/IV, mesenchymal stem cell intravenous injection ,MSC/BI, mesenchymal stem cell bronchial administration ,IL, Interleukin ,Biology (General) ,PGC1a, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ,BM, Bone marrow ,H&E, Haemotoxylin and eosin ,OVA, Ovalbumin ,FIS1, Mitochondrial fission 1 protein ,Cys-LT, Cysteinyl Leukotriene ,respiratory system ,TGF, Transforming growth factor ,PBS, Phosphate-buffered saline ,IFN, Interferon ,HLA, Human leukocyte antigen ,medicine.anatomical_structure ,ELISA, Enzyme-linked immunosorbent assay ,Original Article ,ATP, Adenosine triphosphate ,medicine.symptom ,Signal transduction ,CD, Cluster of differentiation ,General Agricultural and Biological Sciences ,MIP, macrophage inflammatory protein ,PAS, Periodic-acid-Schiff ,MMP, Matrix metalloproteinase ,TFAM, Transcription factor A mitochondrial ,QH301-705.5 ,Inflammation ,COX, Cyclooxygenase ,NO, Nitric oxide ,MSC ,iPSC, induced pluripotent stem cells ,Nrf, Nuclear erythroid 2 p45-related factor ,CCL, Chemokine (C-C motif) ligand ,medicine ,BALF, Bronchoalveolar lavage fluid ,LT, Leukotriene ,Th, T helper ,Asthma ,ComputingMethodologies_COMPUTERGRAPHICS ,Goblet cell ,Drp1, Mitochondrial fission depends on the cytosolic GTPase dynamin-related protein 1 ,business.industry ,Mesenchymal stem cell ,TNF, Tumor necrosis factor ,Eosinophil ,HGF, Hepatocyte growth factor ,medicine.disease ,Mucus ,respiratory tract diseases ,HO, Heme oxygenase ,Immune system ,IDO, Indoleamine 2,3-dioxygenase ,Immunology ,business ,ND1, NADH-ubiquinone oxidoreductase chain 1 ,ROS, Reactive oxygen species - Abstract
Graphical abstract, Asthma is a complicated lung disease, which has increased morbidity and mortality rates in worldwide. There is an overlap between asthma pathophysiology and mitochondrial dysfunction and MSCs may have regulatory effect on mitochondrial dysfunction and treats asthma. Therefore, immune-modulatory effect of MSCs and mitochondrial signaling pathways in asthma was studied. After culturing of MSCs and producing asthma animal model, the mice were treated with MSCs via IV via IT. BALf's eosinophil Counting, The levels of IL-4, −5, −13, −25, –33, INF-γ, Cys-LT, LTB4, LTC4, mitochondria genes expression of COX-1, COX-2, ND1, Nrf2, Cytb were measured and lung histopathological study were done. BALf's eosinophils, the levels of IL-4, −5, −13, −25, –33, LTB4, LTC4, Cys-LT, the mitochondria genes expression (COX-1, COX-2, Cytb and ND-1), perivascular and peribronchial inflammation, mucus hyper-production and hyperplasia of the goblet cell in pathological study were significantly decreased in MSCs-treated asthma mice and reverse trend was found about Nrf-2 gene expression, IFN-γ level and ratio of the INF-γ/IL-4. MSC therapy can control inflammation, immune-inflammatory factors in asthma and mitochondrial related genes, and prevent asthma immune-pathology.
- Published
- 2021