496 results on '"PTR-ToF-MS"'
Search Results
2. A novel VOC breath tracer method to evaluate indoor respiratory exposures in the near- and far-fields; implications for the spread of respiratory viruses
- Author
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Parhizkar, Hooman, Fretz, Mark, Laguerre, Aurélie, Stenson, Jason, Corsi, Richard L, Van Den Wymelenberg, Kevin G, and Gall, Elliott T
- Subjects
Epidemiology ,Public Health ,Health Sciences ,Lung ,Clinical Research ,2.2 Factors relating to the physical environment ,Aetiology ,Good Health and Well Being ,Air Pollutants ,Air Pollution ,Indoor ,Breath Tests ,Volatile Organic Compounds ,Aerosols ,Disease Transmission ,Infectious ,Bioaerosol ,COVID-19 ,Healthy buildings ,Risk ,Infectious disease ,PTR-ToF-MS ,Chemical Sciences ,Environmental Sciences ,Medical and Health Sciences ,Public health - Abstract
BackgroundSeveral studies suggest that far-field transmission (>6 ft) explains a significant number of COVID-19 superspreading outbreaks.ObjectiveTherefore, quantifying the ratio of near- and far-field exposure to emissions from a source is key to better understanding human-to-human airborne infectious disease transmission and associated risks.MethodsIn this study, we used an environmentally-controlled chamber to measure volatile organic compounds (VOCs) released from a healthy participant who consumed breath mints, which contained unique tracer compounds. Tracer measurements were made at 0.76 m (2.5 ft), 1.52 m (5 ft), 2.28 m (7.5 ft) from the participant, as well as in the exhaust plenum of the chamber.ResultsWe observed that 0.76 m (2.5 ft) trials had ~36-44% higher concentrations than other distances during the first 20 minutes of experiments, highlighting the importance of the near-field exposure relative to the far-field before virus-laden respiratory aerosol plumes are continuously mixed into the far-field. However, for the conditions studied, the concentrations of human-sourced tracers after 20 minutes and approaching the end of the 60-minute trials at 0.76 m, 1.52 m, and 2.28 m were only ~18%, ~11%, and ~7.5% higher than volume-averaged concentrations, respectively.SignificanceThis study suggests that for rooms with similar airflow parameters disease transmission risk is dominated by near-field exposures for shorter event durations (e.g., initial 20-25-minutes of event) whereas far-field exposures are critical throughout the entire event and are increasingly more important for longer event durations.Impact statementWe offer a novel methodology for studying the fate and transport of airborne bioaerosols in indoor spaces using VOCs as unique proxies for bioaerosols. We provide evidence that real-time measurement of VOCs can be applied in settings with human subjects to estimate the concentration of bioaerosol at different distances from the emitter. We also improve upon the conventional assumption that a well-mixed room exhibits instantaneous and perfect mixing by addressing spatial distances and mixing over time. We quantitatively assessed the exposure levels to breath tracers at alternate distances and provided more insights into the changes on "near-field to far-field" ratios over time. This method can be used in future to estimate the benefits of alternate environmental conditions and occupant behaviors.
- Published
- 2023
3. Influence of the Drying Process on the Volatile Profile of Different Capsicum Species.
- Author
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Taiti, Cosimo, Comparini, Diego, Moscovini, Lavinia, Violino, Simona, Costa, Corrado, and Mancuso, Stefano
- Subjects
PEPPERS ,HOT peppers ,SWEET peppers ,SPECIES ,VOLATILE organic compounds ,FOOD safety ,AROMATIC compounds - Abstract
Chili is a globally significant spice used fresh or dried for culinary, condiment, and medicinal purposes. Growing concerns about food safety have increased the demand for high-quality products and non-invasive tools for quality control like origin tracing and safety assurance. Volatile analysis offers a rapid, comprehensive, and safe method for characterizing various food products. Thus, this study aims to assess the impact of the drying process on the aromatic composition of various Capsicum species and to identify key compounds driving the aromatic complexity of each genetic makeup. To accomplish these objectives, the aroma was examined in fruits collected from 19 different pepper accessions (Capsicum sp.) belonging to four species: one ancestral (C. chacoense) and three domesticated pepper species (C. annuum, C. baccatum and C. chinense). Fresh and dried samples were analyzed using a headspace PTR-TOF-MS platform. Our findings reveal significant changes in the composition and concentration of volatile organic compounds (VOCs) from fresh to dried Capsicum. Notably, chili peppers of the species C. chinense consistently exhibited higher emission intensity and a more complex aroma compared to other species (both fresh and dried). Overall, the data clearly demonstrate that the drying process generally leads to a reduction in the intensity and complexity of the aromatic compounds emitted. Specifically, fresh peppers showed higher volatile organic compounds content compared to dried ones, except for the two sweet peppers studied, which exhibited the opposite behavior. Our analysis underscores the variability in the effect of drying on volatile compound composition among different pepper species and even among different cultivars, highlighting key compounds that could facilitate species classification in dried powder. This research serves as a preliminary guide for promoting the utilization of various pepper species and cultivars as powder, enhancing product valorization. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. A Study on the Formation Reactions and Conversion Mechanisms of HONO and HNO 3 in the Atmosphere of Daejeon, Korea.
- Author
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Kim, Kyoungchan, Lee, Chunsang, Choi, Dayeong, Han, Sangwoo, Eom, Jiwon, and Han, Jinseok
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NITROGEN oxides , *ATMOSPHERE , *NITROUS acid , *VOLATILE organic compounds , *RADICALS (Chemistry) - Abstract
Nitrogen oxides (NOX) in the atmosphere cause oxidation reactions with photochemical radicals and volatile organic compounds, leading to the accumulation of ozone (O3). NOX constitutes a significant portion of the NOy composition, with nitrous acid (HONO) and nitric acid (HNO3) following. HONO plays a crucial role in the reaction cycle of NOX and hydrogen oxides. The majority of HNO3 reduction mechanisms result from aerosolization through heterogeneous reactions, having adverse effects on humans and plants by increasing secondary aerosol concentrations in the atmosphere. The investigation of the formation and conversion mechanisms of HONO and HNO3 is important; however, research in this area is currently lacking. In this study, we observed HONO, HNO3, and their precursor gases were observed in the atmosphere using parallel-plate diffusion scrubber-ion chromatography. A 0-D box model simulated the compositional distribution of NOy in the atmosphere. The formation reactions and conversion mechanisms of HONO and HNO3 were quantified using reaction equations and reaction coefficients. Among the various mechanisms, dominant mechanisms were identified, suggesting their importance. According to the calculation results, the produce of HONO was predominantly attributed to heterogeneous reactions, excluding an unknown source. The sink processes were mainly governed by photolysis during daytime and reactions with OH radicals during nighttime. HNO3 showed dominance in its production from N2O5, and in its conversion mechanisms primarily involving aerosolization and deposition. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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5. Maize landrace and post-harvest traits are reflected in the volatile profile and nutritional composition of Italian maize porridge (Polenta): A preliminary study
- Author
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Michele Pedrotti, Linarty Linarty, Peng Cleo, Teresa Oliviero, Vincenzo Fogliano, and Franco Biasioli
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Flint maize ,Polenta ,Volatilome ,SPME GC-MS ,PTR-TOF-MS ,Food processing and manufacture ,TP368-456 - Abstract
Maize porridge, known as ''polenta'' in Italy, is a global staple food. This study aims to characterize the quality of four Italian flint maize landraces by investigating physical properties and macronutrients composition. By using SPME GC–MS and PTR-ToF-MS we analyzed the flours volatilome and changes in aroma profile post- cooking. Cooking induced the formation of 5 compounds and the loss of up to 25 compounds, primarily through evaporation. Post-cooking, the concentrations of some sulphur compounds (methanethiol, dimethyl sulfide and dimethyl trisulfide), lipid oxidation compounds (2-pentylfuran and hexanal) and Maillard reaction compounds including some aldehydes (nonanal, benzaldehyde, phenylacetaldheyde), pyridine and furans (furfural and furfuryl alcohol) increased. Differences in volatilome and macronutrients contents among landraces were also observed with Marano samples having on average a significantly higher concentration of proteins (13.67 %), while the Nostrano samples had the highest fat content (5.00 %). Fatty acid profile differences were mirrored in the volatilome. Spin flours had the highest level of linoleic acid, leading to elevated levels in cooked polenta due to linoleic acid oxidation. The differences in volatilome and macronutrients contents among the samples confirmed that local landraces are not only important for biodiversity and cultural heritage but also lead to unique aroma compounds profiles.
- Published
- 2024
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6. INVESTIGATIONS OF POTENTIALLY HARMFUL LIMONENE PHOTOOXIDATION PRODUCTS UNDER NOx AND NOx-FREE ATMOSPHERIC SIMULATED CONDITIONS.
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Negru, Alina Giorgiana, Roman, Claudiu, Amarandei, Cornelia, Arsene, Cecilia, and Olariu, Romeo Iulian
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PROTON transfer reactions , *LIMONENE , *WEATHER , *TIME-of-flight mass spectrometers , *GAS phase reactions , *PHOTOOXIDATION , *QUARTZ - Abstract
Given the ubiquity of limonene in cleaning and cosmetic products, investigating its gasphase oxidation products from reactions with OH radicals and ozone has become important for human health. The present study investigates limonene gas-phase reaction with OH radicals under controlled NOx or NOx-free conditions using facilities from the 760 L Environmental Simulation Chamber made of Quartz (ESC-Q-UAIC) together with state-of-the-art instruments such as proton-transfer-reaction time-of-flight mass spectrometer (PTR-ToF-MS, model 6000 X2, IONICON). The obtained kinetic results (klimonene+OH = (16.42 ± 2.20) × 10-11 cm³×molecule-1×s-1), based on the relative rate techniques with 1,3,5-trimethylbenzene and cyclohexene as reference compounds, are in good agreement with the previous kinetic studies. The PTR-ToF-MS mass spectra acquired within the NOx-free experiments revealed signals at mass-to-charge ratios of 101.060, 139.112, and 169.122, that may be assigned to potentially harmful oxidation products, namely 4-oxopentanal (C5H8O2), 4-acetyl-1-methylcyclohexene (C9H14O), and 3-isopropenyl-6-oxo-heptanal (C10H16O2), respectively. Additionally, observed differences between NOx and NOx-free experiments highlight that NOx strongly suppresses the formation of secondary organic aerosols during limonene oxidation and favours the formation of 4-acetyl-1-methylcyclohexene. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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7. Identification of Volatile Molecules and Bioactivity of Gruyt Craft Beer Enriched with Citrus aurantium var. dulcis L. Essential Oil.
- Author
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Taiti, Cosimo, Di Sotto, Antonella, Stefano, Giovanni, Percaccio, Ester, Iannone, Matteo, Marianelli, Andrea, and Garzoli, Stefania
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CRAFT beer , *ESSENTIAL oils , *TANNINS , *TERPENES , *IRON chelates , *VOLATILE organic compounds , *PLANT polyphenols , *FOOD aroma , *CITRUS - Abstract
In this work, for the first time, a gruyt beer and the same one after the addition of Citrus aurantium essential oil (AEO), were investigated to determine the composition of the volatile fraction. The applied analytical techniques, such as Head Space/Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry (HS/SPME-GC-MS) and Proton Transfer Reaction-Time of Flight-Mass Spectrometer (PTR-ToF-MS), allowed us to identify the content of volatile organic compounds (VOCs). From the comparison between the two beer samples, it showed that the one after the addition of AEO was particularly richened in limonene and a series of minor terpene compounds. AEO was also characterized by GC/MS analysis and the results showed that limonene reached 95%. Confocal microscopy was used to look at riboflavin autofluorescence in yeast cells. It was found that beer with AEO had twice as much fluorescence intensity as the control. A spectrophotometric analysis of total polyphenols, tannins, and flavonoids, and a bioactivity screening, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azinobis-(3-Ethylbenzthiazolin-6-Sulfonic Acid) (ABTS) radical scavenger, chelating, reducing, antiglycative ones, were also carried out. Moreover, the tolerability of the tested samples in human H69 cholangiocytes and the cytoprotection towards the tert-butyl hydroperoxide (tBOOH)-induced oxidative damage were evaluated. Under our experimental conditions, the beers were found to be able to scavenge DPPH and ABTS radicals and chelate iron ions, despite weak antiglycative and reducing properties. The tested samples did not affect the viability of H69 cholangiocytes up to the highest concentrations; moreover, no signs of cytoprotection against the damage induced by tBOOH were highlighted. Adding AEO to beer resulted in a moderate enhancement of its DPPH scavenging and chelating abilities, without improvements in the other assays. Conversely, AEO and its major compound limonene were ineffective when assessed at the concentrations added to beer. This evidence suggests that the addition of AEO may enhance the organoleptic features of the beer and slightly potentiate some of its bioactivities. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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8. Detection of Secondary Metabolites, Proximate Composition and Bioactivity of Organic Dried Spirulina (Arthrospira platensis).
- Author
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Taiti, Cosimo, Di Vito, Maura, Di Mercurio, Mattia, Costantini, Lara, Merendino, Nicolò, Sanguinetti, Maurizio, Bugli, Francesca, and Garzoli, Stefania
- Subjects
METABOLITES ,LACTOBACILLUS casei ,SPIRULINA ,OXIDANT status ,CARBOHYDRATES ,SUGAR alcohols ,VOLATILE organic compounds - Abstract
In this work, Arthrospira platensis grown in Tuscany, Italy, was investigated using different analytical approaches to characterize its volatile and non-volatile chemical composition. The results showed the presence of a high number of volatile organic compounds (VOCs) such as hydrocarbons, furans, sulfides, alkanes, aldehydes, alcohols, ketones, esters and compounds belonging to other chemical classes such as fatty acids, alcohols and sugars. Furthermore, a proximal composition analysis was also performed to determine the protein, fat, carbohydrate and ash content. Total antioxidant capacity (TAC) determined by FRAP and ABTS
•+ methods (5.96 mmol TE/g DW; 5.28 mmol Fe2+ E/g DW, respectively), showed good reducing power and comparable free radical scavenging activity. The antibacterial power of spirulina-based alcoholic macerate (AM) was also evaluated against Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 060127853), Enterococcus faecalis (ATCC 29211), Klebsiella pneumoniae (ATCC 700603) and Candida albicans (ATCC 24433) and the obtained data have shown that it had no effect against pathogenic bacterial strains. On the contrary, at low concentrations, AM exerted a prebiotic effect on some probiotic strains such as L. casei if treated with AM concentrations ranging from 1.56% v/v and 3.12% v/v and L. rhamnosus if treated with AM concentrations lower than 0.78% v/v. In conclusion, this study highlighted how spirulina, based on the rich composition and its antioxidant and prebiotic effect, can represent a source of beneficial substances for human health. [ABSTRACT FROM AUTHOR]- Published
- 2024
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9. Leaf-level metabolic changes in response to drought affect daytime CO2 emission and isoprenoid synthesis pathways.
- Author
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Ladd, S Nemiah, Daber, L Erik, Bamberger, Ines, Kübert, Angelika, Kreuzwieser, Jürgen, Purser, Gemma, Ingrisch, Johannes, Deleeuw, Jason, Haren, Joost van, Meredith, Laura K, and Werner, Christiane
- Subjects
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LEGUMES , *DROUGHTS , *AMINO acid synthesis , *KREBS cycle , *ATMOSPHERIC chemistry , *ISOPENTENOIDS , *TROPICAL forests - Abstract
In the near future, climate change will cause enhanced frequency and/or severity of droughts in terrestrial ecosystems, including tropical forests. Drought responses by tropical trees may affect their carbon use, including production of volatile organic compounds (VOCs), with implications for carbon cycling and atmospheric chemistry that are challenging to predict. It remains unclear how metabolic adjustments by mature tropical trees in response to drought will affect their carbon fluxes associated with daytime CO2 production and VOC emission. To address this gap, we used position-specific 13C-pyruvate labeling to investigate leaf CO2 and VOC fluxes from four tropical species before and during a controlled drought in the enclosed rainforest of Biosphere 2 (B2). Overall, plants that were more drought-sensitive had greater reductions in daytime CO2 production. Although daytime CO2 production was always dominated by non-mitochondrial processes, the relative contribution of CO2 from the tricarboxylic acid cycle tended to increase under drought. A notable exception was the legume tree Clitoria fairchildiana R.A. Howard, which had less anabolic CO2 production than the other species even under pre-drought conditions, perhaps due to more efficient refixation of CO2 and anaplerotic use for amino acid synthesis. The C. fairchildiana was also the only species to allocate detectable amounts of 13C label to VOCs and was a major source of VOCs in B2. In C. fairchildiana leaves, our data indicate that intermediates from the mevalonic acid (MVA) pathway are used to produce the volatile monoterpene trans -β-ocimene, but not isoprene. This apparent crosstalk between the MVA and methylerythritol phosphate pathways for monoterpene synthesis declined with drought. Finally, although trans -β-ocimene emissions increased under drought, it was increasingly sourced from stored intermediates and not de novo synthesis. Unique metabolic responses of legumes may play a disproportionate role in the overall changes in daytime CO2 and VOC fluxes in tropical forests experiencing drought. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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10. Room temperature rate coefficients for the reaction of chlorine atoms with a series of volatile methylsiloxanes (L2‐L5, D3‐D6).
- Author
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Sarachandra Kumar Geetha, Anjitha, Mikoviny, Tomas, Piel, Felix, and Wisthaler, Armin
- Subjects
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TIME-of-flight mass spectrometry , *PROTON transfer reactions , *CHLORINE , *ATOMS , *ATMOSPHERIC temperature , *ATMOSPHERIC pressure - Abstract
The kinetics of chlorine (Cl) atom reactions with a series of volatile methylsiloxanes (VMS) including hexamethyldisiloxane (L2), octamethyltrisiloxane (L3), decamethyltetrasiloxane (L4), dodecamethylpentasiloxane (L5), hexamethylcyclotrisiloxane (D3), octamethylcyclotetrasiloxane (D4), decamethylcyclopentasiloxane (D5), and dodecamethylcyclohexasiloxane (D6) were investigated in relative rate experiments at room temperature and atmospheric pressure. The experiments were carried out in a 0.2 m3 PFA Teflon chamber, employing proton‐transfer‐reaction time‐of‐flight mass spectrometry (PTR‐ToF‐MS) as the chemical‐analytical tool. The following relative and absolute reaction rate coefficients were obtained using isoprene as reference compound (kVMS/kisoprene; kVMS × 1010 cm3 molec−1 s −1): L2 (0.32 ± 0.02; 1.31 ± 0.21), L3 (0.38 ± 0.00; 1.56 ± 0.23), L4 (0.48 ± 0.01; 1.98 ± 0.29), L5 (0.54 ± 0.03; 2.22 ± 0.34), D3 (0.14 ± 0.02; 0.56 ± 0.13), D4 (0.26 ± 0.01; 1.05 ± 0.16), D5 (0.36 ± 0.02; 1.46 ± 0.22), D6 (0.39 ± 0.02; 1.61 ± 0.25). The following relative and absolute reaction rate coefficients were obtained using toluene as reference compound (kVMS/ktoluene; kVMS × 1010 cm3 molec−1 s −1): L2 (1.59 ± 0.18; 0.95 ± 0.14), L3 (2.25 ± 0.14; 1.35 ± 0.16), L4 (2.38 ± 0.01; 1.43 ± 0.14), L5 (3.57 ± 0.11; 2.14 ± 0.22), D3 (0.87 ± 0.01; 0.52 ± 0.05), D4 (1.48 ± 0.12; 0.89 ± 0.11), D5 (2.02 ± 0.15; 1.21 ± 0.15), D6 (2.54 ± 0.11; 1.52 ± 0.17). Our data confirm that reactions with Cl atoms need to be taken into account when assessing the decomposition of VMS in Cl‐rich tropospheric environments. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
11. Room temperature rate coefficients for the reaction of chlorine atoms with a series of volatile methylsiloxanes (L2‐L5, D3‐D6).
- Author
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Sarachandra Kumar Geetha, Anjitha, Mikoviny, Tomas, Piel, Felix, and Wisthaler, Armin
- Subjects
TIME-of-flight mass spectrometry ,PROTON transfer reactions ,CHLORINE ,ATOMS ,ATMOSPHERIC temperature ,ATMOSPHERIC pressure - Abstract
The kinetics of chlorine (Cl) atom reactions with a series of volatile methylsiloxanes (VMS) including hexamethyldisiloxane (L2), octamethyltrisiloxane (L3), decamethyltetrasiloxane (L4), dodecamethylpentasiloxane (L5), hexamethylcyclotrisiloxane (D3), octamethylcyclotetrasiloxane (D4), decamethylcyclopentasiloxane (D5), and dodecamethylcyclohexasiloxane (D6) were investigated in relative rate experiments at room temperature and atmospheric pressure. The experiments were carried out in a 0.2 m3 PFA Teflon chamber, employing proton‐transfer‐reaction time‐of‐flight mass spectrometry (PTR‐ToF‐MS) as the chemical‐analytical tool. The following relative and absolute reaction rate coefficients were obtained using isoprene as reference compound (kVMS/kisoprene; kVMS × 1010 cm3 molec−1 s −1): L2 (0.32 ± 0.02; 1.31 ± 0.21), L3 (0.38 ± 0.00; 1.56 ± 0.23), L4 (0.48 ± 0.01; 1.98 ± 0.29), L5 (0.54 ± 0.03; 2.22 ± 0.34), D3 (0.14 ± 0.02; 0.56 ± 0.13), D4 (0.26 ± 0.01; 1.05 ± 0.16), D5 (0.36 ± 0.02; 1.46 ± 0.22), D6 (0.39 ± 0.02; 1.61 ± 0.25). The following relative and absolute reaction rate coefficients were obtained using toluene as reference compound (kVMS/ktoluene; kVMS × 1010 cm3 molec−1 s −1): L2 (1.59 ± 0.18; 0.95 ± 0.14), L3 (2.25 ± 0.14; 1.35 ± 0.16), L4 (2.38 ± 0.01; 1.43 ± 0.14), L5 (3.57 ± 0.11; 2.14 ± 0.22), D3 (0.87 ± 0.01; 0.52 ± 0.05), D4 (1.48 ± 0.12; 0.89 ± 0.11), D5 (2.02 ± 0.15; 1.21 ± 0.15), D6 (2.54 ± 0.11; 1.52 ± 0.17). Our data confirm that reactions with Cl atoms need to be taken into account when assessing the decomposition of VMS in Cl‐rich tropospheric environments. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
12. Application of breath analysis for the detection of drugs of abuse
- Author
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Mirmigkou, Sofia
- Subjects
breath analysis ,breath profiles ,human breath ,breath volatiles ,volatile organic compounds (VOCs) ,detection of drugs of abuse ,PTR-TOF-MS - Abstract
Human breath is a mixture of gases of which a small part is volatile organic compounds (VOCs), which can either be of endogenous or exogenous origin. This thesis focuses on the exogenous compounds that are metabolized in the human body, their detection in exhaled breath and their possible application as a forensic tool in the detection of drugs of abuse. Online real-time proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS) was used for the analysis of breath after having consumed the equivalent of one unit of alcohol as a model system. These experiments showed ethanol could be very quickly detected and its metabolites appeared in the breath profile sometime later, showing the significance of mouth contamination while measuring ethanol via breath. Large differences were also observed when comparing the results between the fasted and non-fasted states of volunteers. Variability of ethanol in the breath profiles was also investigated, showing the importance of biological variation in such studies. The investigation of the ion distribution of alcohols using selective reagent ionization time-of-flight mass spectrometry (SRI-TOF-MS) is presented to better determine breath compounds produced via drug metabolism. The ion distribution of a selection of deuterated compounds was also investigated, showing the attention that needs to be taken when working with such compounds as markers in breath profiles. The PTR-TOF-MS system was applied further for the investigation of a more complex drug of abuse in breath, pseudoephedrine. Breath and blood profiles of drug metabolites were investigated and showed how variable the T_max can be in blood samples, but also the homogeneity of breath profiles between volunteers making it difficult to decipher differences in the data set. Such studies show the challenging aspects of breath analysis as a detection method for drugs of abuse as biological variation is a major confounder. However, whilst the use of breath analysis should be approached with some caution it has potential for future forensic applications.
- Published
- 2021
- Full Text
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13. Influence of the Drying Process on the Volatile Profile of Different Capsicum Species
- Author
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Cosimo Taiti, Diego Comparini, Lavinia Moscovini, Simona Violino, Corrado Costa, and Stefano Mancuso
- Subjects
Capsicum ,DRYING process ,PTR-TOF-MS ,PLSDA ,volatile analysis ,Botany ,QK1-989 - Abstract
Chili is a globally significant spice used fresh or dried for culinary, condiment, and medicinal purposes. Growing concerns about food safety have increased the demand for high-quality products and non-invasive tools for quality control like origin tracing and safety assurance. Volatile analysis offers a rapid, comprehensive, and safe method for characterizing various food products. Thus, this study aims to assess the impact of the drying process on the aromatic composition of various Capsicum species and to identify key compounds driving the aromatic complexity of each genetic makeup. To accomplish these objectives, the aroma was examined in fruits collected from 19 different pepper accessions (Capsicum sp.) belonging to four species: one ancestral (C. chacoense) and three domesticated pepper species (C. annuum, C. baccatum and C. chinense). Fresh and dried samples were analyzed using a headspace PTR-TOF-MS platform. Our findings reveal significant changes in the composition and concentration of volatile organic compounds (VOCs) from fresh to dried Capsicum. Notably, chili peppers of the species C. chinense consistently exhibited higher emission intensity and a more complex aroma compared to other species (both fresh and dried). Overall, the data clearly demonstrate that the drying process generally leads to a reduction in the intensity and complexity of the aromatic compounds emitted. Specifically, fresh peppers showed higher volatile organic compounds content compared to dried ones, except for the two sweet peppers studied, which exhibited the opposite behavior. Our analysis underscores the variability in the effect of drying on volatile compound composition among different pepper species and even among different cultivars, highlighting key compounds that could facilitate species classification in dried powder. This research serves as a preliminary guide for promoting the utilization of various pepper species and cultivars as powder, enhancing product valorization.
- Published
- 2024
- Full Text
- View/download PDF
14. Addition of Spirulina to Craft Beer: Evaluation of the Effects on Volatile Flavor Profile and Cytoprotective Properties.
- Author
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Taiti, Cosimo, Stefano, Giovanni, Percaccio, Ester, Di Giacomo, Silvia, Iannone, Matteo, Marianelli, Andrea, Di Sotto, Antonella, and Garzoli, Stefania
- Subjects
VITAMIN B2 ,SPIRULINA ,CYTOPROTECTION ,BEER ,CRAFT beer ,RADICALS (Chemistry) ,FLAVOR ,CARBOXYLIC acids ,OXIDATIVE stress - Abstract
SPME-GC-MS and PTR-ToF-MS techniques were applied to describe the content of volatile flavor compounds in a craft beer before and after adding spirulina. The obtained results showed that the volatile profile of the two beer samples differed. Furthermore, to chemically characterize biomass spirulina, a derivatization reaction followed by GC-MS analysis was performed, highlighting a high content of molecules belonging to different chemical classes, such as sugars, fatty acids and carboxylic acids. A spectrophotometric analysis of total polyphenols and tannins, investigation into the scavenging activity towards DPPH and ABTS radicals and confocal microscopy of brewer's yeast cells were carried out. Moreover, the cytoprotective and antioxidant properties towards the oxidative damage induced by tert-butyl hydroperoxide (tBOOH) in human H69 cholangiocytes were investigated. Finally, the modulation of Nrf2 signaling under oxidative stress conditions was also evaluated. Both samples of beer were shown to contain similar levels of total polyphenols and tannins, with slightly increased levels in that containing spirulina 0.25% w/v. Moreover, the beers were found to be endowed with radical scavenging properties towards both DPPH and ABTS radicals, albeit with a weak contribution of spirulina; however, a higher riboflavin content was detected in spirulina-treated yeast cells. Conversely, the addition of spirulina (0.25% w/v) appeared to improve the cytoprotective properties of beer towards tBOOH-induced oxidative damage in H69 cells and reduce intracellular oxidative stress. Accordingly, the cytosolic Nrf2 expression was found to be increased. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
15. Selective Reagent Ion-Time-of-Flight-Mass Spectrometric Investigations of the Intravenous Anaesthetic Propofol and Its Major Metabolite 2,6-Diisopropyl-1,4-benzoquinone.
- Author
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Chawaguta, Anesu, Weiss, Florentin, Marotto, Alessandro, Jürschik, Simone, and Mayhew, Chris A.
- Subjects
PROPOFOL ,QUINONE ,METHYL groups ,PROTON affinity ,DAUGHTER ions ,CHARGE transfer ,QUINONE derivatives - Abstract
Featured Application: First detailed reduced electric field selected reagent ion mass spectrometric study of propofol and its major metabolite for potential analytical breath test applications in the clinical environment. The first detailed selected reagent ion-time-of-flight-mass spectrometric fundamental investigations of 2,6-diisopropylphenol, more commonly known as propofol (C
12 H18 O), and its metabolite 2,6-diisopropyl-1,4-benzoquinone (C12 H16 O2 ) using the reagent ions H3 O+ , H3 O+ .H2 O, O2 +• and NO+ are reported. Protonated propofol is the dominant product ion resulting from the reaction of H3 O+ with propofol up to a reduced electric field strength (E/N) of about 170 Td. After 170 Td, collision-induced dissociation leads to protonated 2-(1-methylethyl)-phenol (C9 H13 O+ ), resulting from the elimination of C3 H6 from protonated propofol. A sequential loss of C3 H6 from C9 H13 O+ also through collision-induced processes leads to protonated phenol (C6 H7 O+ ), which becomes the dominant ionic species at E/N values exceeding 170 Td. H3 O+ .H2 O does not react with propofol via a proton transfer process. This is in agreement with our calculated proton affinity of propofol being 770 kJ mol−1 . Both O2 +• and NO+ react with propofol via a charge transfer process leading to two product ions, C12 H18 O+ (resulting from non-dissociative charge transfer) and C11 H15 O+ that results from the elimination of one of the methyl groups from C12 H18 O+ . This dissociative pathway is more pronounced for O2 +• than for NO+ throughout the E/N range investigated (approximately 60–210 Td), which reflects the higher recombination energy of O2 +• (12.07 eV) compared to that of NO+ (9.3 eV), and hence the higher internal energy deposited into the singly charged propofol. Of the four reagent ions investigated, only H3 O+ and H3 O+ .H2 O react with 2,6-diisopropyl-1,4-benzoquinone, resulting in only the protonated parent at all E/N values investigated. The fundamental ion-molecule studies reported here provide underpinning information that is of use for the development of soft chemical ionisation mass spectrometric analytical techniques to monitor propofol and its major metabolite in the breath. The detection of propofol in breath has potential applications for determining propofol blood concentrations during surgery and for elucidating metabolic processes in real time. [ABSTRACT FROM AUTHOR]- Published
- 2023
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16. Investigation of Geraniol Biotransformation by Commercial Saccharomyces Yeast Strains by Two Headspace Techniques: Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry (SPME-GC/MS) and Proton Transfer Reaction-Time of Flight-Mass Spectrometry (PTR-ToF-MS)
- Author
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Roberts, Rebecca, Khomenko, Iuliia, Eyres, Graham T., Bremer, Phil, Silcock, Patrick, Betta, Emanuela, and Biasioli, Franco
- Subjects
MASS spectrometry ,GAS chromatography ,BIOCONVERSION ,YEAST ,VOLATILE organic compounds ,SPECTROMETRY ,SACCHAROMYCES - Abstract
Hop-derived volatile organic compounds (VOCs) and their transformation products significantly impact beer flavour and aroma. Geraniol, a key monoterpene alcohol in hops, has been reported to undergo yeast-modulated biotransformation into various terpenoids during fermentation, which impacts the citrus and floral aromas of the finished beer. This study monitored the evolution of geraniol and its transformation products throughout fermentation to provide insight into differences as a function of yeast species and strain. The headspace concentration of VOCs produced during fermentation in model wort was measured using Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry (SPME-GC/MS) and Proton Transfer Reaction-Time of Flight-Mass Spectrometry (PTR-ToF-MS). In the absence of yeast, only geraniol was detected, and no terpenoid compounds were detected in geraniol-free ferments. During fermentation, the depletion of geraniol was closely followed by the detection of citronellol, citronellyl acetate and geranyl acetate. The concentration of the products and formation behaviour was yeast strain dependent. SPME-GC/MS provided confidence in compound identification. PTR-ToF-MS allowed online monitoring of these transformation products, showing when formation differed between Saccharomyces cerevisiae and Saccharomyces pastorianus yeasts. A better understanding of the ability of different yeast to biotransform hop terpenes will help brewers predict, control, and optimize the aroma of the finished beer. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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17. Detection of Secondary Metabolites, Proximate Composition and Bioactivity of Organic Dried Spirulina (Arthrospira platensis)
- Author
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Cosimo Taiti, Maura Di Vito, Mattia Di Mercurio, Lara Costantini, Nicolò Merendino, Maurizio Sanguinetti, Francesca Bugli, and Stefania Garzoli
- Subjects
GC/MS ,PTR-ToF-MS ,antioxidant activity ,antimicrobial activity ,prebiotic effect ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
In this work, Arthrospira platensis grown in Tuscany, Italy, was investigated using different analytical approaches to characterize its volatile and non-volatile chemical composition. The results showed the presence of a high number of volatile organic compounds (VOCs) such as hydrocarbons, furans, sulfides, alkanes, aldehydes, alcohols, ketones, esters and compounds belonging to other chemical classes such as fatty acids, alcohols and sugars. Furthermore, a proximal composition analysis was also performed to determine the protein, fat, carbohydrate and ash content. Total antioxidant capacity (TAC) determined by FRAP and ABTS•+ methods (5.96 mmol TE/g DW; 5.28 mmol Fe2+E/g DW, respectively), showed good reducing power and comparable free radical scavenging activity. The antibacterial power of spirulina-based alcoholic macerate (AM) was also evaluated against Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 060127853), Enterococcus faecalis (ATCC 29211), Klebsiella pneumoniae (ATCC 700603) and Candida albicans (ATCC 24433) and the obtained data have shown that it had no effect against pathogenic bacterial strains. On the contrary, at low concentrations, AM exerted a prebiotic effect on some probiotic strains such as L. casei if treated with AM concentrations ranging from 1.56% v/v and 3.12% v/v and L. rhamnosus if treated with AM concentrations lower than 0.78% v/v. In conclusion, this study highlighted how spirulina, based on the rich composition and its antioxidant and prebiotic effect, can represent a source of beneficial substances for human health.
- Published
- 2023
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18. Identification of Volatile Molecules and Bioactivity of Gruyt Craft Beer Enriched with Citrus aurantium var. dulcis L. Essential Oil
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Cosimo Taiti, Antonella Di Sotto, Giovanni Stefano, Ester Percaccio, Matteo Iannone, Andrea Marianelli, and Stefania Garzoli
- Subjects
chemical composition ,HS-SPME ,GC-MS ,PTR-ToF-MS ,antioxidant activity ,chelating activity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
In this work, for the first time, a gruyt beer and the same one after the addition of Citrus aurantium essential oil (AEO), were investigated to determine the composition of the volatile fraction. The applied analytical techniques, such as Head Space/Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry (HS/SPME-GC-MS) and Proton Transfer Reaction-Time of Flight-Mass Spectrometer (PTR-ToF-MS), allowed us to identify the content of volatile organic compounds (VOCs). From the comparison between the two beer samples, it showed that the one after the addition of AEO was particularly richened in limonene and a series of minor terpene compounds. AEO was also characterized by GC/MS analysis and the results showed that limonene reached 95%. Confocal microscopy was used to look at riboflavin autofluorescence in yeast cells. It was found that beer with AEO had twice as much fluorescence intensity as the control. A spectrophotometric analysis of total polyphenols, tannins, and flavonoids, and a bioactivity screening, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azinobis-(3-Ethylbenzthiazolin-6-Sulfonic Acid) (ABTS) radical scavenger, chelating, reducing, antiglycative ones, were also carried out. Moreover, the tolerability of the tested samples in human H69 cholangiocytes and the cytoprotection towards the tert-butyl hydroperoxide (tBOOH)-induced oxidative damage were evaluated. Under our experimental conditions, the beers were found to be able to scavenge DPPH and ABTS radicals and chelate iron ions, despite weak antiglycative and reducing properties. The tested samples did not affect the viability of H69 cholangiocytes up to the highest concentrations; moreover, no signs of cytoprotection against the damage induced by tBOOH were highlighted. Adding AEO to beer resulted in a moderate enhancement of its DPPH scavenging and chelating abilities, without improvements in the other assays. Conversely, AEO and its major compound limonene were ineffective when assessed at the concentrations added to beer. This evidence suggests that the addition of AEO may enhance the organoleptic features of the beer and slightly potentiate some of its bioactivities.
- Published
- 2023
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19. Volatilomics of raspberry fruit germplasm by combining chromatographic and direct-injection mass spectrometric techniques
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Brian Farneti, Iuliia Khomenko, Matteo Ajelli, Karen Elizabeth Wells, Emanuela Betta, Eugenio Aprea, Lara Giongo, and Franco Biasioli
- Subjects
Rubus idaeus L. ,PTR-ToF-MS ,SPME-GC-MS ,germplasm ,VOCs ,phenotyping ,Biology (General) ,QH301-705.5 - Abstract
The application of direct-injection mass spectrometric (DI-MS) techniques, like Proton Transfer Reaction Time of Flight Mass Spectrometry (PTR-ToF-MS) has been suggested as a reliable phenotyping tool for fruit volatilome assessment in both genetic and quality-related studies. In this study the complexity of raspberry aroma was investigated by a comprehensive untargeted VOC analysis, done by combining SPME-GC-MS and PTR-ToF-MS assessments with multi-block discriminant analysis using the DIABLO mixOmics framework. The aim was to acquire an exhaustive characterization of the raspberry volatilome according to different fruit ripening stages (pink, ripe, and overripe) and genetic variances (50 accessions), as well as to investigate the potential of PTR-ToF-MS as a rapid and high throughput VOC phenotyping tool to address issues related to raspberry fruit quality. Results of this study demonstrated the complementarity between SPME-GC-MS and PTR-ToF-MS techniques to evaluate the raspberry aroma composition. PTR-ToF-MS generates reliable raspberry VOC fingerprints mainly due to a reduced compound fragmentation and precise content estimation. In addition, the high collinearity between isomers of monoterpenes and norisoprenoids, discovered by GC analysis, reduces the main analytic limitation of PTR-ToF-MS of not being able to separate isomeric molecules. The high similarity between the VOC matrices obtained by applying PTR-ToF-MS and SPME-GC-MS confirmed the possibility of using PTR-ToF-MS as a reliable high throughput phenotyping tool for raspberry volatiolome assessment. In addition, results provided by the germplasm collection investigation enabled to distinguish the best performing accessions, based on VOCs composition, to be used as superior parental lines for future breeding programs.
- Published
- 2023
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20. Rapid detection of markers in green coffee beans with different primary processing treatments of Coffea arabica L. from Yunnan.
- Author
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Wan, Li, Li, Yan, Wang, Hong, Wang, Yueping, Song, Lianping, and Liang, Wenjuan
- Subjects
- *
GREEN bean , *COFFEE beans , *COFFEE , *LINOLEIC acid , *RAPID tooling , *COFFEE processing - Abstract
The characteristic flavor of Coffea arabica from Yunnan is largely attributed to the primary processing treatments through affecting the VOCs accumulation. Therefore, a rapid and comprehensive detection technique is needed to accurately recognize VOCs in green coffee beans with different pretreatment methods. Hence, we conducted volatile profiles and identified nine markers of three different primary processed green coffee beans from the major production areas in Yunnan with the combined of HS-SPME-GC-MS and PTR-TOF-MS. The relationships between the chemical composition and the content of VOCs in green coffee beans were elucidated. Among the markers, palmitic acid (F3), linoleic acid (F6), α -ethylidene phenylacetaldehyde (T4), and phytane (T8) contributed to the antioxidant activity of sun-exposed green coffee beans. In conclusion, the analytical technology presented here provided a general tool for an overall and rapid understanding of a detailed volatile profiles of green coffee beans in Yunnan. • Characterizing chemical classes in different primary processed green coffee beans. • Identified nine markers in different processed green coffee beans from three origins. • Confirmed the viability of rapid recognition of markers in green coffee beans. • Possible contributors of antioxidant activity were found in VOCs and markers. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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21. Monitoring In Vitro and In Vivo Aroma Release of Espresso Coffees with Proton-Transfer-Reaction Time-of-Flight Mass Spectrometry.
- Author
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Romano, Andrea, Cappellin, Luca, Bogialli, Sara, Pastore, Paolo, Navarini, Luciano, and Biasioli, Franco
- Subjects
FOOD aroma ,ESPRESSO ,TIME-of-flight mass spectrometry ,COFFEE - Abstract
This work presents in vitro and in vivo aroma release analysis of three espresso coffees carried out by PTR-ToF-MS headspace and nosespace analysis, respectively. The products were C. arabica coffees prepared using an espresso coffee machine: a low-caffeine C. arabica var. laurina light roast, a low-caffeine C. arabica var. laurina dark roast, and a single-origin coffee from Ethiopia which were roasted to a medium roast degree. Headspace analysis allowed for discrimination between coffees with a prediction accuracy of 92% or higher. Relevant discriminating compounds were related to the roasting degree and varietal compounds. Coffee nosespace consisted of 35 mass peaks overall. Despite this relatively low number of detected peaks, coffee discrimination was still possible with ≥93% accuracy. The compounds most relevant to the discrimination were those related to the roasting degree. Major differences—both qualitative and quantitative—were found between headspace and nosespace profiles. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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22. Soil VOC emissions of a Mediterranean woodland are sensitive to shrub invasion.
- Author
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Meischner, M., Haberstroh, S., Daber, L. E., Kreuzwieser, J., Caldeira, M. C., Schnitzler, J.‐P., and Werner, C.
- Subjects
- *
TIME-of-flight mass spectrometry , *SOIL respiration , *ACETALDEHYDE , *CORK oak , *SOILS , *FORESTS & forestry , *ACETONE - Abstract
Many belowground processes, such as soil respiration and soil–atmosphere VOC (volatile organic compounds) exchange, are closely linked to soil microbiological processes. However, little is known about how changes in plant species cover, i.e. after plant invasion, alter these soil processes. In particular, the response of soil VOC emissions to plant invasion is not well understood.We analysed soil VOC emissions and soil respiration of a Mediterranean cork oak (Quercus suber) ecosystem, comparing soil VOC emissions from a non‐invaded Q. suber woodland to one invaded by the shrub Cistus ladanifer. Soil VOC emissions were determined under controlled conditions using online proton‐transfer time‐of‐flight mass spectrometry. Net soil VOC emissions were measured by exposing soils with or without litter to different temperature and soil moisture conditions.Soil VOC emissions were sensitive to C. ladanifer invasion. Highest net emission rates were determined for oxygenated VOC (acetaldehyde, acetone, methanol, acetic acid), and high temperatures enhanced total VOC emissions. Invasion affected the relative contribution of various VOC. Methanol and acetaldehyde were emitted exclusively from litter and were associated with the non‐invaded sites. In contrast, acetone emissions increased in response to shrub presence. Interestingly, low soil moisture enhanced the effect of shrub invasion on VOC emissions.Our results indicate that shrub invasion substantially influences important belowground processes in cork oak ecosystems, in particular soil VOC emissions. High soil moisture is suggested to diminish the invasion effect through a moisture‐induced increase in microbial decomposition rates of soil VOC. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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23. Development and validation of a screening model for lung cancer using machine learning: A large-scale, multi-center study of biomarkers in breath.
- Author
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Jing Li, Yuwei Zhang, Qing Chen, Zhenhua Pan, Jun Chen, Meixiu Sun, Junfeng Wang, Yingxin Li, and Qing Ye
- Subjects
LUNG cancer ,PROTON transfer reactions ,MACHINE learning ,TIME-of-flight mass spectrometry ,MODEL validation - Abstract
Objectives: Lung cancer (LC) is the largest single cause of death from cancer worldwide, and the lack of effective screening methods for early detection currently results in unsatisfactory curative treatments. We herein aimed to use breath analysis, a noninvasive and very simple method, to identify and validate biomarkers in breath for the screening of lung cancer. Materials and methods: We enrolled a total of 2308 participants from two centers for online breath analyses using proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS). The derivation cohort included 1007 patients with primary LC and 1036 healthy controls, and the external validation cohort included 158 LC patients and 107 healthy controls. We used eXtreme Gradient Boosting (XGBoost) to create a panel of predictive features and derived a prediction model to identify LC. The optimal number of features was determined by the greatest area under the receiver-operating characteristic (ROC) curve (AUC). Results: Six features were defined as a breath-biomarkers panel for the detection of LC. In the training dataset, the model had an AUC of 0.963 (95% CI, 0.941-0.982), and a sensitivity of 87.1% and specificity of 93.5% at a positivity threshold of 0.5. Our model was tested on the independent validation dataset and achieved an AUC of 0.771 (0.718-0.823), and sensitivity of 67.7% and specificity of 73.0%. Conclusion: Our results suggested that breath analysis may serve as a valid method in screening lung cancer in a borderline population prior to hospital visits. Although our breath-biomarker panel is noninvasive, quick, and simple to use, it will require further calibration and validation in a prospective study within a primary care setting. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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24. Evaluation of tetrachloroethylene (PCE) and its degradation products in human exhaled breath and indoor air in a community setting.
- Author
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Lee JH, Bryant AK, Alajlouni M, Boor BE, Tasoglou A, and Liu S
- Subjects
- Humans, Male, Female, Adult, Exhalation, Middle Aged, Environmental Exposure analysis, Indiana, Environmental Monitoring methods, Breath Tests methods, Air Pollution, Indoor analysis, Tetrachloroethylene analysis
- Abstract
Tetrachloroethylene (PCE) is a widely utilized volatile chemical in industrial applications, including dry cleaning and metal degreasing. Exposure to PCE potentially presents a significant health risk to workers as well as communities near contamination sites. Adverse health effects arise not only from PCE, but also from PCE degradation products, such as trichloroethylene (TCE) and vinyl chloride (VC). PCE, TCE, and VC can contaminate water, soil, and air, leading to exposure through multiple pathways, including inhalation, ingestion, and dermal contact. This study focused on a community setting in Martinsville, Indiana, a working-class Midwestern community in the United States, where extensive PCE contamination has occurred due to multiple contamination sites (referring to 'plumes'), including a Superfund site. Utilizing proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS), PCE, TCE, and VC concentrations were measured in the exhaled breath of 73 residents from both within and outside the plume areas. PCE was detected in 66 samples, TCE in 26 samples, and VC in 68 samples. Our results revealed a significant positive correlation between the concentrations of these compounds in exhaled breath and indoor air (Pearson correlation coefficients: PCE = 0.75, TCE = 0.71, and VC = 0.89). This study confirms the presence of PCE and its degradation products in exhaled breath in a community exposure investigation, demonstrating the potential of using exhaled breath analysis in monitoring exposure to environmental contaminants. This study showed the feasibility of utilizing PTR-TOF-MS in community investigations to assess exposure to PCE and its degradation products by measuring these compounds in exhaled breath and indoor air., (Creative Commons Attribution license.)
- Published
- 2024
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25. Non-Invasive O-Toluidine Monitoring during Regional Anaesthesia with Prilocaine and Detection of Accidental Intravenous Injection in an Animal Model.
- Author
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Brock, Beate, Fuchs, Patricia, Kamysek, Svend, Walther, Udo, Traxler, Selina, Pugliese, Giovanni, Miekisch, Wolfram, Schubert, Jochen K., and Trefz, Phillip
- Subjects
INTRAVENOUS injections ,PRILOCAINE ,TOLUIDINE ,CERVICAL plexus ,ANESTHESIA ,INTRACRANIAL pressure - Abstract
Regional anaesthesia is well established as a standard method in clinical practice. Currently, the local anaesthetics of amino-amide types such as prilocaine are frequently used. Despite routine use, complications due to overdose or accidental intravenous injection can arise. A non-invasive method that can indicate such complications early would be desirable. Breath gas analysis offers great potential for the non-invasive monitoring of drugs and their volatile metabolites. The physicochemical properties of o-toluidine, the main metabolite of prilocaine, allow its detection in breath gas. Within this study, we investigated whether o-toluidine can be monitored in exhaled breath during regional anaesthesia in an animal model, if correlations between o-toluidine and prilocaine blood levels exist and if accidental intravenous injections are detectable by o-toluidine breath monitoring. Continuous o-toluidine monitoring was possible during regional anaesthesia of the cervical plexus and during simulated accidental intravenous injection of prilocaine. The time course of exhaled o-toluidine concentrations considerably differed depending on the injection site. Intravenous injection led to an immediate increase in exhaled o-toluidine concentrations within 2 min, earlier peak and higher maximum concentrations, followed by a faster decay compared to regional anaesthesia. The strength of correlation of blood and breath parameters depended on the injection site. In conclusion, real time monitoring of o-toluidine in breath gas is possible by means of PTR-ToF-MS. Since simulated accidental intravenous injection led to an immediate increase in exhaled o-toluidine concentrations within 2 min and higher maximum concentrations, monitoring exhaled o-toluidine may potentially be applied for the non-invasive real-time detection of accidental intravenous injection of prilocaine. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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- View/download PDF
26. Dynamic aromatics: Evaluating fragrance quality shifts and implementation of real-time rapid detection in Hedychium cut flowers during senescence.
- Author
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Zhou, Yiwei, Wang, Qin, Wei, Xue, Gao, Ting, Abbas, Farhat, Yan, Fulong, He, Jingjuan, Yu, Yunyi, Yu, Rangcai, and Fan, Yanping
- Subjects
- *
CUT flowers , *MULTIVARIATE analysis , *PHENYLPROPANOIDS , *LINALOOL , *MONOTERPENOIDS - Abstract
The postharvest fragrance quality of aromatic cut flowers is crucial, yet efficient and rapid detection methods are lacking. To develop a rapid and accurate method for evaluating the fragrance quality during the senescence of aromatic cut flowers, this study first utilized HS–SPME–GC–MS to analyze the fragrance of six Hedychium cultivars cut flowers at different stages postharvest (bud, semi-open, full bloom, and senescence), identifying 69 compounds. Multivariate statistical analysis revealed significant during full bloom, with monoterpenoids predominant in 'ZS', 'EM', 'Gaoling', and 'Jin', and benzenoids/phenylpropanoids in 'MH'. Concurrently, PTR–ToF–MS detected 68 masses consistent with GC–MS results. PLS–DA analysis identified eucalyptol, benzyl acetate, linalool, (E)- β -ocimene, methyl benzoate, and masses m/z 91.057, m/z 137.134, m/z 81.07, m/z 102.096, m/z 31.019 as potential markers for distinguishing Hedychium varieties. Additionally, PLS analysis identified masses m/z 58.078, m/z 103.076, and m/z 155.144 as predictors of agarospirol content; m/z 73.065, m/z 74.069, m/z 151.151 for eucalyptol, and m/z 27.021, m/z 31.019, m/z 41.039, m/z 43.054, m/z 55.054, m/z 57.033, m/z 70.077, m/z 72.056, m/z 88.079, m/z 91.057, m/z 92.065, and m/z 108.089 for benzyl acetate. The results provide new methods for efficient fragrance evaluation during the postharvest senescence of aromatic flower. [Display omitted] • Identified 69 VOCs in six Hedychium cut flowers using HS–SPME–GC–MS. • PTR–ToF–MS effectively evaluates fragrance changes in Hedychium cut flowers. • Combining GC–MS and PTR–ToF–MS enhances floral scent detection efficiency. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
27. The characteristic VOCs of different parts of Artocarpus heterophyllus fruit based on HS-SPME-GC-MS and PTR-TOF-MS.
- Author
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Wang, Yueping, Huang, Yequn, Song, Lianping, Wang, Hong, Wan, Li, Pang, Jinqian, and Liang, Wenjuan
- Subjects
- *
JACKFRUIT , *OCTANOIC acid , *WASTE recycling , *WASTE management , *ETHYL esters - Abstract
Insight investigation on both edible pulps and inedible parts involving inflorescence axis and shreds of Artocarpus heterophyllus Lam were carried out, a total of 98 VOCs and 201 masses were identified by the combination of HS-SPME-GC-MS and PTR-TOF-MS. Among them, according to the consistency of OAV and results of VIP > 1, p < 0.05, compounds methyl isovalerate (A2), 3-methylbutyl acetate (A5) and octanoic acid, ethyl ester (A21) were recognized as aroma markers to distinguish the pulps, shreds and inflorescence axis. Meanwhile, the inflorescence axis (IC 50 : 1.82 mg/mL) and shreds (IC 50 : 16.74 mg/mL) exhibited more excellent antioxidant potency than pulps (IC 50 : 17.43 mg/mL) in vitro. These findings validated the feasibility of coupling HS-SPME-GC-MS and PTR-TOF-MS for rapid detection of characteristic VOCs of this plant, and offered new prospect of fragrance utilization and waste management of the edible and inedible parts of A. heterophyllus fruit. [Display omitted] • HS-SPME-GC–MS and PTR-TOF-MS were coupled in identifying VOCs profiles of jackfruit. • Aroma markers to distinguish three parts of A. heterophyllus fruit were recognized. • VOCs from inedible parts of jackfruit showed potency in fragrance development. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
28. Determination of personal exposure to volatile organic compounds and their health risks after the use of mosquito repellents in residential environments using passive sampling.
- Author
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Kim, Pil-Gon, Choi, Yun-Hee, Lee, Arum, Shin, Jaeho, Song, Eugene, Sochichiu, Stefana, Koo, Yerim, Hong, Yongseok, and Kwon, Jung-Hwan
- Published
- 2024
- Full Text
- View/download PDF
29. Evolution of isoprene emission in Arecaceae (palms)
- Author
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Mingai Li, Jia Xu, Fuling Lyu, Iuliia Khomenko, Franco Biasioli, Mariacristina Villani, Barbara Baldan, and Claudio Varotto
- Subjects
Arecaceae ,isoprene ,isoprene synthase ,IspS diagnostic tetrad ,PTR‐ToF‐MS ,site‐directed mutagenesis ,Evolution ,QH359-425 - Abstract
Abstract Isoprene synthase (IspS) is the sole enzyme in plants responsible for the yearly emission in the atmosphere of thousands of tonnes of the natural hydrocarbon isoprene worldwide. Species of the monocotyledonous family Arecaceae (palms) are among the highest plant emitters, but to date no IspS gene from this family has been identified. Here, we screened with PTR‐ToF‐MS 18 genera of the Arecaceae for isoprene emission and found that the majority of the sampled species emits isoprene. Putative IspS genes from six different genera were sequenced and three of them were functionally characterized by heterologous overexpression in Arabidopsis thaliana, demonstrating that they encode functional IspS genes. Site‐directed mutagenesis and expression in Arabidopsis demonstrated the functional relevance of a novel IspS diagnostic tetrad from Arecaceae, whose most variable amino acids could not preserve catalytic function when substituted by a putatively dicotyledonous‐specific tetrad. In particular, mutation of threonine 479 likely impairs the open–closed transition of the enzyme by altering the network of hydrogen bonds between helices H1α, H, and I. These results shed new light on the evolution of IspS in monocots, suggesting that isoprene emission is an ancestral trait within the Arecaceae family. The identification of IspS from Arecaceae provides promising novel enzymes for the production of isoprene in heterologous systems and allows the screening and selection of commercially relevant palm varieties with lower environmental impact.
- Published
- 2021
- Full Text
- View/download PDF
30. Addition of Spirulina to Craft Beer: Evaluation of the Effects on Volatile Flavor Profile and Cytoprotective Properties
- Author
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Cosimo Taiti, Giovanni Stefano, Ester Percaccio, Silvia Di Giacomo, Matteo Iannone, Andrea Marianelli, Antonella Di Sotto, and Stefania Garzoli
- Subjects
SPME-GC-MS ,PTR-ToF-MS ,chemical analyses ,radical scavenger activity ,oxidative stress ,cytoprotective properties ,Therapeutics. Pharmacology ,RM1-950 - Abstract
SPME-GC-MS and PTR-ToF-MS techniques were applied to describe the content of volatile flavor compounds in a craft beer before and after adding spirulina. The obtained results showed that the volatile profile of the two beer samples differed. Furthermore, to chemically characterize biomass spirulina, a derivatization reaction followed by GC-MS analysis was performed, highlighting a high content of molecules belonging to different chemical classes, such as sugars, fatty acids and carboxylic acids. A spectrophotometric analysis of total polyphenols and tannins, investigation into the scavenging activity towards DPPH and ABTS radicals and confocal microscopy of brewer’s yeast cells were carried out. Moreover, the cytoprotective and antioxidant properties towards the oxidative damage induced by tert-butyl hydroperoxide (tBOOH) in human H69 cholangiocytes were investigated. Finally, the modulation of Nrf2 signaling under oxidative stress conditions was also evaluated. Both samples of beer were shown to contain similar levels of total polyphenols and tannins, with slightly increased levels in that containing spirulina 0.25% w/v. Moreover, the beers were found to be endowed with radical scavenging properties towards both DPPH and ABTS radicals, albeit with a weak contribution of spirulina; however, a higher riboflavin content was detected in spirulina-treated yeast cells. Conversely, the addition of spirulina (0.25% w/v) appeared to improve the cytoprotective properties of beer towards tBOOH-induced oxidative damage in H69 cells and reduce intracellular oxidative stress. Accordingly, the cytosolic Nrf2 expression was found to be increased.
- Published
- 2023
- Full Text
- View/download PDF
31. Selective Reagent Ion-Time-of-Flight-Mass Spectrometric Investigations of the Intravenous Anaesthetic Propofol and Its Major Metabolite 2,6-Diisopropyl-1,4-benzoquinone
- Author
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Anesu Chawaguta, Florentin Weiss, Alessandro Marotto, Simone Jürschik, and Chris A. Mayhew
- Subjects
PTR-ToF-MS ,SRI-ToF-MS ,propofol ,2,6 diisopropyl-1,4-benzoquinone ,intravenous anaesthetic ,reduced electric field ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
The first detailed selected reagent ion-time-of-flight-mass spectrometric fundamental investigations of 2,6-diisopropylphenol, more commonly known as propofol (C12H18O), and its metabolite 2,6-diisopropyl-1,4-benzoquinone (C12H16O2) using the reagent ions H3O+, H3O+.H2O, O2+• and NO+ are reported. Protonated propofol is the dominant product ion resulting from the reaction of H3O+ with propofol up to a reduced electric field strength (E/N) of about 170 Td. After 170 Td, collision-induced dissociation leads to protonated 2-(1-methylethyl)-phenol (C9H13O+), resulting from the elimination of C3H6 from protonated propofol. A sequential loss of C3H6 from C9H13O+ also through collision-induced processes leads to protonated phenol (C6H7O+), which becomes the dominant ionic species at E/N values exceeding 170 Td. H3O+.H2O does not react with propofol via a proton transfer process. This is in agreement with our calculated proton affinity of propofol being 770 kJ mol−1. Both O2+• and NO+ react with propofol via a charge transfer process leading to two product ions, C12H18O+ (resulting from non-dissociative charge transfer) and C11H15O+ that results from the elimination of one of the methyl groups from C12H18O+. This dissociative pathway is more pronounced for O2+• than for NO+ throughout the E/N range investigated (approximately 60–210 Td), which reflects the higher recombination energy of O2+• (12.07 eV) compared to that of NO+ (9.3 eV), and hence the higher internal energy deposited into the singly charged propofol. Of the four reagent ions investigated, only H3O+ and H3O+.H2O react with 2,6-diisopropyl-1,4-benzoquinone, resulting in only the protonated parent at all E/N values investigated. The fundamental ion-molecule studies reported here provide underpinning information that is of use for the development of soft chemical ionisation mass spectrometric analytical techniques to monitor propofol and its major metabolite in the breath. The detection of propofol in breath has potential applications for determining propofol blood concentrations during surgery and for elucidating metabolic processes in real time.
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- 2023
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32. Investigation of Geraniol Biotransformation by Commercial Saccharomyces Yeast Strains by Two Headspace Techniques: Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry (SPME-GC/MS) and Proton Transfer Reaction-Time of Flight-Mass Spectrometry (PTR-ToF-MS)
- Author
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Rebecca Roberts, Iuliia Khomenko, Graham T. Eyres, Phil Bremer, Patrick Silcock, Emanuela Betta, and Franco Biasioli
- Subjects
beer ,fermentation ,geraniol ,biotransformation ,SPME-GC/MS ,PTR-ToF-MS ,Fermentation industries. Beverages. Alcohol ,TP500-660 - Abstract
Hop-derived volatile organic compounds (VOCs) and their transformation products significantly impact beer flavour and aroma. Geraniol, a key monoterpene alcohol in hops, has been reported to undergo yeast-modulated biotransformation into various terpenoids during fermentation, which impacts the citrus and floral aromas of the finished beer. This study monitored the evolution of geraniol and its transformation products throughout fermentation to provide insight into differences as a function of yeast species and strain. The headspace concentration of VOCs produced during fermentation in model wort was measured using Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry (SPME-GC/MS) and Proton Transfer Reaction-Time of Flight-Mass Spectrometry (PTR-ToF-MS). In the absence of yeast, only geraniol was detected, and no terpenoid compounds were detected in geraniol-free ferments. During fermentation, the depletion of geraniol was closely followed by the detection of citronellol, citronellyl acetate and geranyl acetate. The concentration of the products and formation behaviour was yeast strain dependent. SPME-GC/MS provided confidence in compound identification. PTR-ToF-MS allowed online monitoring of these transformation products, showing when formation differed between Saccharomyces cerevisiae and Saccharomyces pastorianus yeasts. A better understanding of the ability of different yeast to biotransform hop terpenes will help brewers predict, control, and optimize the aroma of the finished beer.
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- 2023
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33. Volatile organic compounds in wintertime North China Plain: Insights from measurements of proton transfer reaction time-of-flight mass spectrometer (PTR-ToF-MS).
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He, Xianjun, Yuan, Bin, Wu, Caihong, Wang, Sihang, Wang, Chaomin, Huangfu, Yibo, Qi, Jipeng, Ma, Nan, Xu, Wanyun, Wang, Ming, Chen, Wentai, Su, Hang, Cheng, Yafang, and Shao, Min
- Subjects
- *
TIME-of-flight mass spectrometers , *VOLATILE organic compounds , *WINTER , *COAL combustion , *FLAME ionization detectors , *PROTON transfer reactions , *FRAGMENTATION reactions , *CHEMICAL ionization mass spectrometry - Abstract
The characteristics of wintertime volatile organic compounds (VOCs) in the North China Plain (NCP) region are complicated and remain obscure. VOC measurements were conducted by a proton transfer reaction time-of-flight mass spectrometer (PTR-ToF-MS) at a rural site in the NCP from November to December 2018. Uncalibrated ions measured by PTR-ToF-MS were quantified and the overall VOC compositions were investigated by combining the measurements of PTR-ToF-MS and gas chromatography-mass spectrometer/flame ionization detector (GC-MS/FID). The measurement showed that although atmospheric VOCs concentrations are often dominated by primary emissions, the secondary formation of oxygenated VOCs (OVOCs) is non-negligible in the wintertime, i.e., OVOCs accounts for 42% ± 7% in the total VOCs (151.3 ± 75.6 ppbV). We demonstrated that PTR-MS measurements for isoprene are substantially overestimated due to the interferences of cycloalkanes. The chemical changes of organic carbon in a pollution accumulation period were investigated, which suggests an essential role of fragmentation reactions for large, chemically reduced compounds during the heavy-polluted stage in wintertime pollution. The changes of emission ratios of VOCs between winter 2011 and winter 2018 in the NCP support the positive effect of "coal to gas" strategies in curbing air pollutants. The high abundances of some key species (e.g. oxygenated aromatics) indicate the strong emissions of coal combustion in wintertime of NCP. The ratio of naphthalene to C8 aromatics was proposed as a potential indicator of the influence of coal combustion on VOCs. [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2022
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34. Detailed Speciation of Non-Methane Volatile Organic Compounds in Exhaust Emissions from Diesel and Gasoline Euro 5 Vehicles Using Online and Offline Measurements.
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Marques, Baptiste, Kostenidou, Evangelia, Valiente, Alvaro Martinez, Vansevenant, Boris, Sarica, Thibaud, Fine, Ludovic, Temime-Roussel, Brice, Tassel, Patrick, Perret, Pascal, Liu, Yao, Sartelet, Karine, Ferronato, Corinne, and D'Anna, Barbara
- Subjects
DIESEL motor exhaust gas ,VOLATILE organic compounds ,DIESEL particulate filters ,PROTON transfer reactions ,TIME-of-flight mass spectrometers ,GASOLINE ,THERMAL desorption - Abstract
The characterization of vehicle exhaust emissions of volatile organic compounds (VOCs) is essential to estimate their impact on the formation of secondary organic aerosol (SOA) and, more generally, air quality. This paper revises and updates non-methane volatile organic compounds (NMVOCs) tailpipe emissions of three Euro 5 vehicles during Artemis cold urban (CU) and motorway (MW) cycles. Positive matrix factorization (PMF) analysis is carried out for the first time on proton transfer reaction time-of-flight mass spectrometer (PTR-ToF-MS) datasets of vehicular emission. Statistical analysis helped to associate the emitted VOCs to specific driving conditions, such as the start of the vehicles, the activation of the catalysts, or to specific engine combustion regimes. Merged PTR-ToF-MS and automated thermal desorption gas chromatography mass spectrometer (ATD-GC-MS) datasets provided an exhaustive description of the NMVOC emission factors (EFs) of the vehicles, thus helping to identify and quantify up to 147 individual compounds. In general, emissions during the CU cycle exceed those during the MW cycle. The gasoline direct injection (GDI) vehicle exhibits the highest EF during both CU and MW cycles (252 and 15 mg/km), followed by the port-fuel injection (PFI) vehicle (24 and 0.4 mg/km), and finally the diesel vehicle (15 and 3 mg/km). For all vehicles, emissions are dominated by unburnt fuel and incomplete combustion products. Diesel emissions are mostly represented by oxygenated compounds (65%) and aliphatic hydrocarbons (23%) up to C
22 , while GDI and PFI exhaust emissions are composed of monoaromatics (68%) and alkanes (15%). Intermediate volatility organic compounds (IVOCs) range from 2.7 to 13% of the emissions, comprising essentially linear alkanes for the diesel vehicle, while naphthalene accounts up to 42% of the IVOC fraction for the gasoline vehicles. This work demonstrates that PMF analysis of PTR-ToF-MS datasets and GC-MS analysis of vehicular emissions provide a revised and deep characterization of vehicular emissions to enrich current emission inventories. [ABSTRACT FROM AUTHOR]- Published
- 2022
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35. Measuring volatile emissions from moss gametophytes: A review of methodologies and new applications.
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Brennan, Danlyn L., Kollar, Leslie M., Kiel, Scott, Deakova, Timea, Laguerre, Aurélie, McDaniel, Stuart F., Eppley, Sarah M., Gall, Elliott T., and Rosenstiel, Todd N.
- Subjects
- *
BIOSPHERE , *GAMETOPHYTES , *PLANT-microbe relationships , *VOLATILE organic compounds , *MOSSES , *SAMPLING (Process) - Abstract
Mosses inhabit nearly all terrestrial ecosystems and engage in important interactions with nitrogen‐fixing microbes, sperm‐dispersing arthropods, and other plants. It is hypothesized that these interactions could be mediated by biogenic volatile organic compounds (BVOCs). Moss BVOCs may play fundamental roles in influencing local ecologies, such as biosphere–atmosphere–hydrosphere communications, physiological and evolutionary dynamics, plant–microbe interactions, and gametophyte stress physiology. Further progress in quantifying the composition, magnitude, and variability of moss BVOC emissions, and their response to environmental drivers and metabolic requirements, is limited by methodological and analytical challenges. We review several sampling techniques with various analytical approaches and describe best practices in generating moss gametophyte BVOC measures. We emphasize the importance of characterizing the composition and magnitude of moss BVOC emissions across a variety of species to better inform and stimulate important cross‐disciplinary studies. We conclude by highlighting how current methods could be employed, as well as best practices for choosing methodologies. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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- View/download PDF
36. Non-Invasive Monitoring of Inflammation in Inflammatory Bowel Disease Patients during Prolonged Exercise via Exhaled Breath Volatile Organic Compounds.
- Author
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Henderson, Ben, Meurs, Joris, Lamers, Carlijn R., Batista, Guilherme Lopes, Materić, Dušan, Bertinetto, Carlo G., Bongers, Coen C. W. G., Holzinger, Rupert, Harren, Frans J. M., Jansen, Jeroen J., Hopman, Maria T. E., and Cristescu, Simona M.
- Subjects
INFLAMMATORY bowel diseases ,EXERCISE intensity ,VOLATILE organic compounds ,PROTON transfer reactions ,TIME-of-flight mass spectrometry ,FITNESS walking ,INFLAMMATION - Abstract
The aim of this study was to investigate volatile organic compounds (VOCs) in exhaled breath as possible non-invasive markers to monitor the inflammatory response in inflammatory bowel disease (IBD) patients as a result of repeated and prolonged moderate-intensity exercise. We included 18 IBD patients and 19 non-IBD individuals who each completed a 30, 40, or 50 km walking exercise over three consecutive days. Breath and blood samples were taken before the start of the exercise event and every day post-exercise to assess changes in the VOC profiles and cytokine concentrations. Proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) was used to measure exhaled breath VOCs. Multivariate analysis, particularly ANOVA-simultaneous component analysis (ASCA), was employed to extract relevant ions related to exercise and IBD. Prolonged exercise induces a similar response in breath butanoic acid and plasma cytokines for participants with or without IBD. Butanoic acid showed a significant correlation with the cytokine IL-6, indicating that butanoic acid could be a potential non-invasive marker for exercise-induced inflammation. The findings are relevant in monitoring personalized IBD management. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
37. Volatile fingerprinting of ripened cheese for authentication and characterisation of different dairy systems
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M. Bergamaschi, A. Cecchinato, and G. Bittante
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cow ,cheese ,volatile organic compound ,ptr-tof-ms ,Animal culture ,SF1-1100 - Abstract
Authentication of dairy systems is of growing interest for the dairy industry and we investigated the potentiality of using volatile fingerprinting of ripened cheeses by proton transfer reaction time-of-flight mass spectrometry. A total of 1,075 individual model cheeses made from milk of individual Brown Swiss cows of 72 farms were analysed. Using a linear discriminant analysis, cows and herds were assigned to 3 or 5 dairy systems differing in management, available facilities, and diets. We obtained variable discrimination abilities (up to 77% of correct classification of cheeses and 70% of farms with cross-validation). We found m/z 61,028 (acetic acid), 109,070 (pyrazine), and m/z 137,132 (terpene) characterising model cheeses from traditional dairy systems and m/z 71,086 (3-methyl-butan-1-ol, 3-methyl-3-buten-1-ol, pentan-1-ol), m/z 101,097 (hexan-2-one, hexanal), m/z 123,117 (nonenal), m/z 129,127 (octan-1-one, octanal), and two unidentified peaks m/z 83,071 and m/z 93,090 characterising model cheeses from the modern farms. In conclusion, it seems possible to discriminate between a range of dairy systems using fast volatile fingerprinting of ripened cheeses but a proper validation of results obtained is needed.Highlights Mass spectrometry technique (PTR-ToF-MS) was able to discriminate between dairy systems. We found m/z 61,028 (acetic acid), 109,070 (pyrazine), and m/z 137,132 (terpene) characterising model cheeses from traditional dairy systems. We found m/z 71,086 (3-methyl-butan-1-ol, 3-methyl-3-buten-1-ol, pentan-1-ol), m/z 101,097 (hexan-2-one, hexanal), m/z 123,117 (nonenal), m/z 129,127 (octan-1-one, octanal), and two unidentified peaks m/z 83,071 and m/z 93,090 characterising model cheeses from the modern farms.
- Published
- 2020
- Full Text
- View/download PDF
38. Measuring volatile emissions from moss gametophytes: A review of methodologies and new applications
- Author
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Danlyn L. Brennan, Leslie M. Kollar, Scott Kiel, Timea Deakova, Aurélie Laguerre, Stuart F. McDaniel, Sarah M. Eppley, Elliott T. Gall, and Todd N. Rosenstiel
- Subjects
biogenic volatile organic compounds ,bryophytes ,gametophytes ,GC‐RGD ,GC‐ToF‐MS ,PTR‐ToF‐MS ,Biology (General) ,QH301-705.5 ,Botany ,QK1-989 - Abstract
Abstract Mosses inhabit nearly all terrestrial ecosystems and engage in important interactions with nitrogen‐fixing microbes, sperm‐dispersing arthropods, and other plants. It is hypothesized that these interactions could be mediated by biogenic volatile organic compounds (BVOCs). Moss BVOCs may play fundamental roles in influencing local ecologies, such as biosphere–atmosphere–hydrosphere communications, physiological and evolutionary dynamics, plant–microbe interactions, and gametophyte stress physiology. Further progress in quantifying the composition, magnitude, and variability of moss BVOC emissions, and their response to environmental drivers and metabolic requirements, is limited by methodological and analytical challenges. We review several sampling techniques with various analytical approaches and describe best practices in generating moss gametophyte BVOC measures. We emphasize the importance of characterizing the composition and magnitude of moss BVOC emissions across a variety of species to better inform and stimulate important cross‐disciplinary studies. We conclude by highlighting how current methods could be employed, as well as best practices for choosing methodologies.
- Published
- 2022
- Full Text
- View/download PDF
39. Jasmonic acid and heat stress induce high volatile organic compound emissions in Picea abies from needles, but not from roots.
- Author
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Meischner M, Dumberger S, Daber LE, Haberstroh S, Kreuzwieser J, Schnitzler JP, and Werner C
- Abstract
Plants emit diverse volatile organic compounds (VOCs) from their leaves and roots for protection against biotic and abiotic stress. An important signaling cascade activated by aboveground herbivory is the jasmonic acid (JA) pathway that stimulates the production of VOCs. So far it remains unclear if the activation of this pathway also leads to enhanced VOC emissions from conifer roots, and how the interplay of above- and belowground defenses in plants are affected by multiple stressors. Therefore, we simultaneously analyzed needle and root VOC emissions of Picea abies saplings, as well as CO2 and H2O fluxes in response to aboveground JA treatment, heat stress and their interaction in a controlled climate chamber experiment. Continuous online VOC measurements by PTR-TOF-MS showed an inverse pattern of total needle and root VOC emissions, when plants were treated with JA and heat. While needle sesquiterpene emissions increased nine-fold one day after JA application, total root VOC emissions decreased. This was mainly due to reduced emissions of acetone and monoterpenes by roots. In response to aboveground JA treatment, root total carbon emitted as VOCs decreased from 31% to only 4%. While VOC emissions aboveground increased, net CO2 assimilation strongly declined due to JA treatment, resulting in net respiration during the day. Interestingly, root respiration was not affected by aboveground JA application. Under heat the effect of JA on VOC emissions of needles and roots was less pronounced. The buffering effect of heat on VOC emissions following JA treatment points towards an impaired defense reaction of the plants under multiple stress. Our results indicate efficient resource allocation within the plant to protect threatened tissues by a rather local VOC release. Roots may only be affected indirectly by reduced belowground carbon allocation, but are not involved directly in the JA-induced stress response., (© The Author(s) 2024. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.)
- Published
- 2024
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40. Insights into the abnormal increase of ozone during COVID-19 in a typical urban city of China.
- Author
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Kun Zhang, Zhiqiang Liu, Xiaojuan Zhang, Qing Li, Jensen, Andrew, Wen Tan, Ling Huang, Yangjun Wang, Joost de Gouw, and Li Li
- Abstract
The outbreak of COVID-19 promoted strict restrictions to human activities in China, which led to dramatic decrease in most air pollutant concentrations (e.g., PM
2.5 , PM10 , NOx , SO2 , and CO). However, abnormal increase of ozone (O3 ) concentrations was found during the lockdown period in most urban areas of China. In this study, we conducted a field measurement targeting ozone and its key precursors by utilizing a novel proton transfer reaction time-of-flight mass spectrometer (PTR-TOF-MS) in Changzhou, which is representative for the Yangtze River Delta (YRD) city cluster of China. We further applied the integrated methodology including machine learning, observation-based model (OBM), and sensitivity analysis to get insights into the reasons causing the abnormal increase of ozone. Major findings include: (1) By deweathered calculation, we found changes in precursor emissions contributed 5.1 ppbv to the observed O3 during the Full-lockdown period, while meteorological conditions only contributed 0.5 ppbv to the O3 changes. (2) By using an OBM model, we found that although significant reduction of O3 precursors was observed during Full-lockdown period, the photochemical formation of O3 was stronger than that during the Pre-lockdown period. (3) The NOx/VOCs ratio dropped dramatically from 1.84 during Pre-lockdown to 0.79 in Full-lockdown period, which switched O3 formation from VOCs-limited regime to the conjunction of NOx- and VOC-limited regime. Additionally, the decrease in NOx/VOCs ratio during Full-lockdown period was supposed to increase the MeanO3 by 2.4 ppbv. Results of this study investigate insights into the relationship between O3 and its precursors in urban area, demonstrating reasons causing the abnormal increase of O3 in most urban areas of China during the COVID-19 lock-down period. This study also underlines the necessity of controlling anthropogenic OVOCs, alkenes, and aromatics in the sustained campaign of reducing O3 pollution in China. [ABSTRACT FROM AUTHOR]- Published
- 2021
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- View/download PDF
41. Monitoring In Vitro and In Vivo Aroma Release of Espresso Coffees with Proton-Transfer-Reaction Time-of-Flight Mass Spectrometry
- Author
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Andrea Romano, Luca Cappellin, Sara Bogialli, Paolo Pastore, Luciano Navarini, and Franco Biasioli
- Subjects
aroma release ,headspace ,nosespace ,espresso coffee ,PTR-ToF-MS ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
This work presents in vitro and in vivo aroma release analysis of three espresso coffees carried out by PTR-ToF-MS headspace and nosespace analysis, respectively. The products were C. arabica coffees prepared using an espresso coffee machine: a low-caffeine C. arabica var. laurina light roast, a low-caffeine C. arabica var. laurina dark roast, and a single-origin coffee from Ethiopia which were roasted to a medium roast degree. Headspace analysis allowed for discrimination between coffees with a prediction accuracy of 92% or higher. Relevant discriminating compounds were related to the roasting degree and varietal compounds. Coffee nosespace consisted of 35 mass peaks overall. Despite this relatively low number of detected peaks, coffee discrimination was still possible with ≥93% accuracy. The compounds most relevant to the discrimination were those related to the roasting degree. Major differences—both qualitative and quantitative—were found between headspace and nosespace profiles.
- Published
- 2022
- Full Text
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42. Emissions of Trace Organic Gases From Western U.S. Wildfires Based on WE‐CAN Aircraft Measurements.
- Author
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Permar, Wade, Wang, Qian, Selimovic, Vanessa, Wielgasz, Catherine, Yokelson, Robert J., Hornbrook, Rebecca S., Hills, Alan J., Apel, Eric C., Ku, I‐Ting, Zhou, Yong, Sive, Barkley C., Sullivan, Amy P., Collett, Jeffrey L., Campos, Teresa L., Palm, Brett B., Peng, Qiaoyun, Thornton, Joel A., Garofalo, Lauren A., Farmer, Delphine K., and Kreidenweis, Sonia M.
- Subjects
VOLATILE organic compounds & the environment ,WILDFIRES ,WILDFIRES & the environment ,AEROSOLS & the environment ,GAS chromatography ,EMISSIONS (Air pollution) - Abstract
We present emission measurements of volatile organic compounds (VOCs) for western U.S. wildland fires made on the NSF/NCAR C‐130 research aircraft during the Western Wildfire Experiment for Cloud Chemistry, Aerosol Absorption, and Nitrogen (WE‐CAN) field campaign in summer 2018. VOCs were measured with complementary instruments onboard the C‐130, including a proton‐transfer‐reaction time‐of‐flight mass spectrometer (PTR‐ToF‐MS) and two gas chromatography (GC)‐based methods. Agreement within combined instrument uncertainties (<60%) was observed for most co‐measured VOCs. GC‐based measurements speciated the isomeric contributions to selected PTR‐ToF‐MS ion masses and generally showed little fire‐to‐fire variation. We report emission ratios (ERs) and emission factors (EFs) for 161 VOCs measured in 31 near‐fire smoke plume transects of 24 specific individual fires sampled in the afternoon when burning conditions are typically most active. Modified combustion efficiency (MCE) ranged from 0.85 to 0.94. The measured campaign‐average total VOC EF was 26.1 ± 6.9 g kg−1, approximately 67% of which is accounted for by oxygenated VOCs. The 10 most abundantly emitted species contributed more than half of the total measured VOC mass. We found that MCE alone explained nearly 70% of the observed variance for total measured VOC emissions (r2 = 0.67) and >50% for 57 individual VOC EFs representing more than half the organic carbon mass. Finally, we found little fire‐to‐fire variability for the mass fraction contributions of individual species to the total measured VOC emissions, suggesting that a single speciation profile can describe VOC emissions for the wildfires in coniferous ecosystems sampled during WE‐CAN. Key Points: A total of 161 measured volatile organic compound emission factors is 26.1 ± 6.9 g kg−1, 67% of which is from oxygenated species76% of the total volatile organic compound emitted mass shows statistically significant dependence on modified combustion efficiencyMass fraction contributions of individual species to the total measured emissions have little variability in 24 western U.S. fires [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
43. Non-Invasive O-Toluidine Monitoring during Regional Anaesthesia with Prilocaine and Detection of Accidental Intravenous Injection in an Animal Model
- Author
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Beate Brock, Patricia Fuchs, Svend Kamysek, Udo Walther, Selina Traxler, Giovanni Pugliese, Wolfram Miekisch, Jochen K. Schubert, and Phillip Trefz
- Subjects
breath gas analysis ,drug monitoring ,o-toluidine ,prilocaine ,PTR-ToF-MS ,regional anaesthesia ,Microbiology ,QR1-502 - Abstract
Regional anaesthesia is well established as a standard method in clinical practice. Currently, the local anaesthetics of amino-amide types such as prilocaine are frequently used. Despite routine use, complications due to overdose or accidental intravenous injection can arise. A non-invasive method that can indicate such complications early would be desirable. Breath gas analysis offers great potential for the non-invasive monitoring of drugs and their volatile metabolites. The physicochemical properties of o-toluidine, the main metabolite of prilocaine, allow its detection in breath gas. Within this study, we investigated whether o-toluidine can be monitored in exhaled breath during regional anaesthesia in an animal model, if correlations between o-toluidine and prilocaine blood levels exist and if accidental intravenous injections are detectable by o-toluidine breath monitoring. Continuous o-toluidine monitoring was possible during regional anaesthesia of the cervical plexus and during simulated accidental intravenous injection of prilocaine. The time course of exhaled o-toluidine concentrations considerably differed depending on the injection site. Intravenous injection led to an immediate increase in exhaled o-toluidine concentrations within 2 min, earlier peak and higher maximum concentrations, followed by a faster decay compared to regional anaesthesia. The strength of correlation of blood and breath parameters depended on the injection site. In conclusion, real time monitoring of o-toluidine in breath gas is possible by means of PTR-ToF-MS. Since simulated accidental intravenous injection led to an immediate increase in exhaled o-toluidine concentrations within 2 min and higher maximum concentrations, monitoring exhaled o-toluidine may potentially be applied for the non-invasive real-time detection of accidental intravenous injection of prilocaine.
- Published
- 2022
- Full Text
- View/download PDF
44. Evolution of isoprene emission in Arecaceae (palms).
- Author
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Li, Mingai, Xu, Jia, Lyu, Fuling, Khomenko, Iuliia, Biasioli, Franco, Villani, Mariacristina, Baldan, Barbara, and Varotto, Claudio
- Subjects
- *
PALMS , *ISOPRENE , *PLANT enzymes , *SITE-specific mutagenesis , *ARABIDOPSIS thaliana , *DATE palm - Abstract
Isoprene synthase (IspS) is the sole enzyme in plants responsible for the yearly emission in the atmosphere of thousands of tonnes of the natural hydrocarbon isoprene worldwide. Species of the monocotyledonous family Arecaceae (palms) are among the highest plant emitters, but to date no IspS gene from this family has been identified. Here, we screened with PTR‐ToF‐MS 18 genera of the Arecaceae for isoprene emission and found that the majority of the sampled species emits isoprene. Putative IspS genes from six different genera were sequenced and three of them were functionally characterized by heterologous overexpression in Arabidopsis thaliana, demonstrating that they encode functional IspS genes. Site‐directed mutagenesis and expression in Arabidopsis demonstrated the functional relevance of a novel IspS diagnostic tetrad from Arecaceae, whose most variable amino acids could not preserve catalytic function when substituted by a putatively dicotyledonous‐specific tetrad. In particular, mutation of threonine 479 likely impairs the open–closed transition of the enzyme by altering the network of hydrogen bonds between helices H1α, H, and I. These results shed new light on the evolution of IspS in monocots, suggesting that isoprene emission is an ancestral trait within the Arecaceae family. The identification of IspS from Arecaceae provides promising novel enzymes for the production of isoprene in heterologous systems and allows the screening and selection of commercially relevant palm varieties with lower environmental impact. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
45. Characteristics of wintertime VOCs in urban Beijing: Composition and source apportionment
- Author
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Liwei Wang, Jay G. Slowik, Yandong Tong, Jing Duan, Yifang Gu, Pragati Rai, Lu Qi, Giulia Stefenelli, Urs Baltensperger, Ru-Jin Huang, Junji Cao, and André S.H. Prévôt
- Subjects
VOCs ,PTR-ToF-MS ,PMF ,SOA formation ,Trajectory clusters ,Environmental pollution ,TD172-193.5 ,Meteorology. Climatology ,QC851-999 - Abstract
Characteristics and sources of volatile organic compounds (VOCs) were investigated with highly time-resolved measurements by a proton-transfer-reaction time-of-flight mass spectrometer (PTR-ToF-MS) at an urban site in Beijing in winter 2017. During the measurement period, high mixing ratios of VOCs (48.9 ppbv) and trace gases were observed, with alternating episodes of strong haze pollution and clean air. Ten VOC families showed clear dependence on the VOC concentration. Aromatics increased the most during haze, with significantly elevated benzene concentration at high VOC concentration, while CxHyO3 and CxHy increased the least. The positive matrix factorization (PMF) receptor model was applied to the VOC mass spectra, yielding four major VOC factors: traffic emissions (21.0%), solid fuel combustion (SFC, 24.4%), and two oxygenated VOC (OVOC) factors (32.3% and 22.3%). Traffic and solid fuel combustion were dominant during the periods of high total VOC concentration, while the OVOC1 fraction was reduced. Comparisons with organic aerosol (OA) sources showed increased oxygenated organic aerosol (OOA) concentration during high VOC concentration periods, indicating the importance of OVOCs to secondary organic aerosol formation. Furthermore, trajectory analysis showed that most of the clean days were associated with northerly winds with high ratios of OVOC1. In contrast, the haze periods were not only due to high primary emissions under stagnant conditions, but also influenced by air masses from a more regional scale.
- Published
- 2021
- Full Text
- View/download PDF
46. Non-Invasive Monitoring of Inflammation in Inflammatory Bowel Disease Patients during Prolonged Exercise via Exhaled Breath Volatile Organic Compounds
- Author
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Ben Henderson, Joris Meurs, Carlijn R. Lamers, Guilherme Lopes Batista, Dušan Materić, Carlo G. Bertinetto, Coen C. W. G. Bongers, Rupert Holzinger, Frans J. M. Harren, Jeroen J. Jansen, Maria T. E. Hopman, and Simona M. Cristescu
- Subjects
inflammatory bowel disease ,exercise ,breath analysis ,PTR-ToF-MS ,volatile organic compounds ,butanoic acid ,Microbiology ,QR1-502 - Abstract
The aim of this study was to investigate volatile organic compounds (VOCs) in exhaled breath as possible non-invasive markers to monitor the inflammatory response in inflammatory bowel disease (IBD) patients as a result of repeated and prolonged moderate-intensity exercise. We included 18 IBD patients and 19 non-IBD individuals who each completed a 30, 40, or 50 km walking exercise over three consecutive days. Breath and blood samples were taken before the start of the exercise event and every day post-exercise to assess changes in the VOC profiles and cytokine concentrations. Proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) was used to measure exhaled breath VOCs. Multivariate analysis, particularly ANOVA-simultaneous component analysis (ASCA), was employed to extract relevant ions related to exercise and IBD. Prolonged exercise induces a similar response in breath butanoic acid and plasma cytokines for participants with or without IBD. Butanoic acid showed a significant correlation with the cytokine IL-6, indicating that butanoic acid could be a potential non-invasive marker for exercise-induced inflammation. The findings are relevant in monitoring personalized IBD management.
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- 2022
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47. High-Throughput Volatilome Fingerprint Using PTR–ToF–MS Shows Species-Specific Patterns in Mortierella and Closely Related Genera.
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Telagathoti, Anusha, Probst, Maraike, Khomenko, Iuliia, Biasioli, Franco, and Peintner, Ursula
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- *
VOLATILE organic compounds , *MORTIERELLA , *HUMAN fingerprints , *MASS spectrometry , *PROTON transfer reactions - Abstract
In ecology, Volatile Organic Compounds (VOCs) have a high bioactive and signaling potential. VOCs are not only metabolic products, but are also relevant in microbial cross talk and plant interaction. Here, we report the first large-scale VOC study of 13 different species of Mortierella sensu lato (s. l.) isolated from a range of different alpine environments. Proton Transfer Reaction–Time-of-Flight Mass Spectrometry (PTR–ToF–MS) was applied for a rapid, high-throughput and non-invasiveVOCfingerprintingof72Mortierellas. l. isolates growing understandardized conditions. Overall, we detected 139 mass peaks in the headspaces of all 13 Mortierellas. l. species studied here. Thus, Mortierella s. l. species generally produce a high number of different VOCs. Mortierella species could clearly bedis criminated basedon their volatilomes, evenifonly high-concentration mass peaks were considered. The volatilomes were partially phylogenetically conserved. There were no VOCs produced by only one species, but the relative concentrations of VOCs differed between species. From a univariate perspective, we detected mass peaks with distinctively high concentrations in single species. Here, we provide initial evidence that VOCs may provide a competitive advantage and modulate Mortierella s. l. species distribution on a global scale. [ABSTRACT FROM AUTHOR]
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- 2021
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- View/download PDF
48. Quantitative and qualitative assessment of VOCs emitted from cellulose acetate movie films by PTR-ToF-MS.
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Kammer, Julien, Truong, Francois, Boissard, Christophe, Soulié, Anne-Laure, Dupont, Anne-Laurence, Simon, Leila, Gros, Valérie, and Lavédrine, Bertrand
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- *
CELLULOSE acetate , *VOLATILE organic compounds , *PROTON transfer reactions , *FORMIC acid , *PROPIONIC acid , *MOTION picture distribution - Abstract
• PTR-ToF-MS was used for the first time for real-time full qualitative and quantitative detection of VOCs released by 41 historical movie films on a cellulose acetate base. • Around 100 different organic ions were attributed to VOCs emitted from films. • Acetic acid, acetaldehyde, acetone, butanol, DMF, formic acid, methanol, propanoic acid dominated the VOC mixture composition. • Over 41 films, acetic acid was the most abundant VOC for 27 films reels, butanol for 6, DMF for 3, formic acid for 2, acetaldehyde for 2, acetone for 1. Cellulose acetate (CA) has been widely used since the second half of the 20th century as a transparent support for photographs and movie films in order to replace the flammable cellulose nitrate. Over time, hydrolysis occurs and the deacetylation of the CA produces acetic acid (AA), a well-known phenomenon in film archives, the so-called "vinegar syndrome". However, beside AA, other off-gassing compounds may be present, and very few studies have been devoted to their quantitative and qualitative assessment. Proton Transfer Reaction "Time-of-Flight" Mass Spectrometer (PTR-ToF-MS) combines high sensitivity with high mass resolution for real-time detection of multiple Volatile Organic Compounds (VOCs). This technique was used to evaluate the air composition from 41 films dated from the second half of the 20th century, which showed different degradation levels (ranked using A-D strips®: level 0–1.5). More than 100 VOCs were detected, and their distribution was different from one film to another. AA was the most abundant VOC in 27 film cans. In others, it was either N,N dimethylformamide (DMF), butanol, acetaldehyde, acetone or formic acid. This study shows that PTR-MS is a powerful tool to monitor in real-time, and non-invasively, objects degradation in a museum environment via the quantitative and qualitative analysis of their VOCs emissions, and that this can be used for hierarchical cluster analysis classification. [ABSTRACT FROM AUTHOR]
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- 2021
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49. Volatile fingerprinting of ripened cheese for authentication and characterisation of different dairy systems.
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Bergamaschi, M., Cecchinato, A., and Bittante, G.
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MASS spectrometry , *TIME-of-flight mass spectrometry , *CHEESE , *FISHER discriminant analysis , *PROTON transfer reactions , *CHEESEMAKING , *ACETIC acid - Abstract
Authentication of dairy systems is of growing interest for the dairy industry and we investigated the potentiality of using volatile fingerprinting of ripened cheeses by proton transfer reaction time-of-flight mass spectrometry. A total of 1,075 individual model cheeses made from milk of individual Brown Swiss cows of 72 farms were analysed. Using a linear discriminant analysis, cows and herds were assigned to 3 or 5 dairy systems differing in management, available facilities, and diets. We obtained variable discrimination abilities (up to 77% of correct classification of cheeses and 70% of farms with cross-validation). We found m/z 61,028 (acetic acid), 109,070 (pyrazine), and m/z 137,132 (terpene) characterising model cheeses from traditional dairy systems and m/z 71,086 (3-methyl-butan-1-ol, 3-methyl-3-buten-1-ol, pentan-1-ol), m/z 101,097 (hexan-2-one, hexanal), m/z 123,117 (nonenal), m/z 129,127 (octan-1-one, octanal), and two unidentified peaks m/z 83,071 and m/z 93,090 characterising model cheeses from the modern farms. In conclusion, it seems possible to discriminate between a range of dairy systems using fast volatile fingerprinting of ripened cheeses but a proper validation of results obtained is needed. Mass spectrometry technique (PTR-ToF-MS) was able to discriminate between dairy systems. We found m/z 61,028 (acetic acid), 109,070 (pyrazine), and m/z 137,132 (terpene) characterising model cheeses from traditional dairy systems. We found m/z 71,086 (3-methyl-butan-1-ol, 3-methyl-3-buten-1-ol, pentan-1-ol), m/z 101,097 (hexan-2-one, hexanal), m/z 123,117 (nonenal), m/z 129,127 (octan-1-one, octanal), and two unidentified peaks m/z 83,071 and m/z 93,090 characterising model cheeses from the modern farms. [ABSTRACT FROM AUTHOR]
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- 2020
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50. Evaluation of the VOC pollution pattern and emission characteristics during the Beijing resurgence of COVID-19 in summer 2020 based on the measurement of PTR-ToF-MS
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Zhining Zhang, Hanyang Man, Fengkui Duan, Zhaofeng Lv, Songxin Zheng, Junchao Zhao, Feifan Huang, Zhenyu Luo, Kebin He, and Huan Liu
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VOC ,Beijing resurgence ,COVID-19 ,PTR-ToF-MS ,source apportionment ,Environmental technology. Sanitary engineering ,TD1-1066 ,Environmental sciences ,GE1-350 ,Science ,Physics ,QC1-999 - Abstract
A second wave of coronavirus disease 2019 (COVID-19) infections emerged in Beijing in summer 2020, which provided an opportunity to explore the response of air pollution to reduced human activity. Proton-transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) coupled with positive matrix factorization (PMF) source apportionment were applied to evaluate the pollution pattern and capture the detailed dynamic emission characteristics of volatile organic compounds (VOCs) during the representative period, with the occurrence of O _3 pollution episodes and the Beijing resurgence of COVID-19. The level of anthropogenic VOC was lower than during the same period in previous years due to the pandemic and emission reduction measures. More than two thirds of the days during the observation period were identified as high-O _3 days and VOCs exhibited higher mixing ratios and faster consumption rates in the daytime on high-O _3 days. The identified VOC emission sources and the corresponding contributions during the whole observation period included: vehicle + fuel (12.41 ± 9.43%), industrial process (9.40 ± 8.65%), solvent usage (19.58 ± 13.46%), biogenic (6.03 ± 5.40%), background + long-lived (5.62 ± 11.37%), and two groups of oxygenated VOC (OVOC) factors (primary emission and secondary formation, 26.14 ± 15.20% and 20.84 ± 14.0%, respectively). Refined dynamic source apportionment results show that the ‘stay at home’ tendency led to decreased emission (−34.47 ± 1.90%) and a weakened morning peak of vehicle + fuel during the Beijing resurgence. However, a growing emission of primary OVOCs (+51.10 ± 8.28%) with similar diurnal variation was observed in the new outbreak and afterwards, which might be related to the enhanced usage of products intended to clean and disinfect. The present study illustrated that more stringent VOC reduction measures towards pandemic products should be carried out to achieve the balanced emission abatement of NO _x and VOC when adhering to regular epidemic prevention and control measures.
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- 2022
- Full Text
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