Search

Your search keyword '"Pahlke, Gudrun"' showing total 49 results
Start Over Author "Pahlke, Gudrun"
49 results on '"Pahlke, Gudrun"'

1. Anthocyanin-Rich Berry Extracts Affect SN-38-Induced Response: A Comparison of Non-Tumorigenic HCEC-1CT and HCT116 Colon Carcinoma Cells.

Author

Schmutz, Cornelia, Plaza, Crepelle, Steiger, Franziska, Stoirer, Natascha, Gufler, Judith, Pahlke, Gudrun, Will, Frank, Berger, Walter, and Marko, Doris

Subjects
POISONS, INTESTINAL mucosa, DNA topoisomerase I, TREATMENT effectiveness, CYTOTOXINS
Abstract

Chemotherapy with irinotecan (CPT-11), the pro-drug of the highly cytotoxic SN-38, is among the standard-of-care treatments for colorectal cancer. To counteract undesired toxic side effects on healthy tissue such as the intestinal epithelium, the use of preparations rich in polyphenols with anti-oxidative and anti-inflammatory properties such as anthocyanins has been proposed. In the present study, the question of whether non-tumorigenic human epithelium cells (HCEC-1CT) can be protected against the cytotoxic impact of SN-38 by anthocyanin-rich polyphenol extracts without compromising the desired therapeutic effect against tumor cells (HCT-116) was addressed. Hence, single and combinatory effects of anthocyanin-rich polyphenol extracts of elderberry (EB), bilberry (Bil), blackberry (BB) and black currant (BC) with the chemotherapeutic drug SN-38 were investigated. Out of the extracts, BB showed the most potent concentration-dependent cytotoxicity alone and in combination with SN-38, with even stronger effects in non-tumorigenic HCEC-1CT cells. In cytotoxic concentrations, BB decreased the level of DNA/topoisomerase I covalent complexes in HCEC-1CT cells below base level but without concomitant reduction in SN-38-induced DNA strand breaks. The herein reported data argue towards an interference of anthocyanins with successful treatment of cancer cells and a lack of protective properties in healthy cells. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

11. Markers for DNA damage are induced in the rat colon by the Alternaria toxin altertoxin-II, but not a complex extract of cultured Alternaria alternata

Author

Aichinger, Georg, primary, Pahlke, Gudrun, additional, Puntscher, Hannes, additional, Groestlinger, Julia, additional, Grabher, Stephanie, additional, Braun, Dominik, additional, Tillmann, Katharina, additional, Plasenzotti, Roberto, additional, Del Favero, Giorgia, additional, Warth, Benedikt, additional, Höger, Harald, additional, and Marko, Doris, additional

Published
2022
Full Text
View/download PDF

17. Long‐term consumption of anthocyanin‐rich fruit juice: impact on gut microbiota and antioxidant markers in lymphocytes of healthy males

Author

Groh, Isabel Anna Maria, Riva, Alessandra, Braun, Dominik, Sutherland, Heidi G., Williams, Owen, Bakuradze, Tamara, Pahlke, Gudrun, Richling, Elke, Haupt, Larisa M., Griffiths, Lyn R., Berry, David, Marko, Doris, Groh, Isabel Anna Maria, Riva, Alessandra, Braun, Dominik, Sutherland, Heidi G., Williams, Owen, Bakuradze, Tamara, Pahlke, Gudrun, Richling, Elke, Haupt, Larisa M., Griffiths, Lyn R., Berry, David, and Marko, Doris

Abstract

Polyphenols are considered protective against diseases associated with oxidative stress. Short‐term intake of an anthocyanin‐rich fruit juice resulted in significantly reduced deoxyribonucleic acid (DNA) strand‐breaks in peripheral blood lymphocytes (PBLs) and affected antioxidant markers in healthy volunteers. Consequently, effects of long‐term consumption of fruit juice are of particular interest. In focus was the impact on nuclear factor erythroid 2 (NFE2)‐related factor 2 (Nrf2), the Nrf2‐regulated genes NAD(P)H quinone oxidoreductase 1 (NQO‐1) and heme oxygenase 1 (HO‐1) as well as effects on the gut microbiota. In a nine‐week placebo‐controlled intervention trial with 57 healthy male volunteers, consumption of anthocyanin‐rich juice significantly increased NQO‐1 and HO‐1 transcript levels in PBLs compared to a placebo beverage as measured by real‐time polymerase chain reaction (PCR). Three Nrf2‐promotor single nucleotide polymorphisms (SNPs), analyzed by pyrosequencing, indicated an association between individual Nrf2 transcript levels and genotype. Moreover, the Nrf2 genotype appeared to correlate with the presence of specific microbial organisms identified by 16S‐PCR and classified as Spirochaetaceae. Furthermore, the microbial community was significantly affected by the duration of juice consumption and intake of juice itself. Taken together, long‐term consumption of anthocyanin‐rich fruit juice affected Nrf2‐dependent transcription in PBLs, indicating systemic effects. Individual Nrf2 genotypes may influence the antioxidant response, thus requiring consideration in future intervention studies focusing on the Nrf2 pathway. Anthocyanin‐rich fruit juice had an extensive impact on the gut microbiota.

Published
2021

20. Long-Term Consumption of Anthocyanin-Rich Fruit Juice: Impact on Gut Microbiota and Antioxidant Markers in Lymphocytes of Healthy Males

Author

Groh, Isabel Anna Maria, primary, Riva, Alessandra, additional, Braun, Dominik, additional, Sutherland, Heidi G., additional, Williams, Owen, additional, Bakuradze, Tamara, additional, Pahlke, Gudrun, additional, Richling, Elke, additional, Haupt, Larisa M., additional, Griffiths, Lyn R., additional, Berry, David, additional, and Marko, Doris, additional

Published
2020
Full Text
View/download PDF

30. First insights into Alternaria multi-toxin in vivo metabolism

Author

Puntscher, Hannes, primary, Hankele, Svenja, additional, Tillmann, Katharina, additional, Attakpah, Eva, additional, Braun, Dominik, additional, Kütt, Mary-Liis, additional, Del Favero, Giorgia, additional, Aichinger, Georg, additional, Pahlke, Gudrun, additional, Höger, Harald, additional, Marko, Doris, additional, and Warth, Benedikt, additional

Published
2019
Full Text
View/download PDF

32. From specialized cell cultures to high quality fine chemicals: ANTHOcyanin production PLatform Using Suspension cultures (ANTHOPLUS)

Author

Matros, Andrea, Mock, Hans-Peter, Oertel, Anne, Martens, Stefan, Wendell, Micael, Hvoslef-Eide, Anne-Kathrine, Pahlke, Gudrun, Marko, Doris, Appelhagen, Ingo, and Martin, Cathie

Subjects
fungi, food and beverages
Abstract

ANTHOPLUS yields anthocyanins in stable plant tissue cultures. We aim to produce purified anthocyanins, of novel complexity in side chain decoration, or labelled with stable isotopes for assaying the composition of feedstock for natural colors, bioavailability, bioefficacy and mechanistic research in experimental medicine and as analytical standards. Production comprises the whole value-chain from synthesis, scale-up, preparative purification, assessment of toxicity and bioactivities to distribution for sales. We focus on strategies established for the medium-scale purification of anthocyanins from cell suspension cultures. Anthocyanin profiles of plant cell extracts and chemical structures of candidate molecules are analyzed by LC-MS. Selected compounds are purified by sequential flash and preparative reversed phase chromatography. The purity of resulting fractions is validated by a combination of LC-MS and GC-MS approaches, as well as by enzyme assays., Journal of International Society of Antioxidants in Nutrition & Health, Vol 3, No 4 (2016)

Published
2016
Full Text
View/download PDF

33. Impact of a blackberry extract and single anthocyanins on the inflammatory response of human macrophage THP-1 cells

Author

Cenk, Ebru, Pahlke, Gudrun, Oertel, Anne, Martens, Stefan, Matros, Andrea, Mock, Hans-Peter, and Marko, Doris

Subjects
Anthocyanins, Inflammation, food and beverages, Settore BIO/15 - BIOLOGIA FARMACEUTICA
Abstract

A diet rich in anthocyanins is considered to be beneficial for health. Thus, investigations on anthocyanin-rich extracts and single constituents are of great interest. We investigated a blackberry extract, containing a spectrum of anthocyanins, in comparison to two sub-fractions comprising cyanidin-3-(6''-dioxalylglucoside and cyanidin-3-(malonylglucoside), respectively, regarding their impact on inflammation-related cytokine expression and secretion in human macrophages. Relative gene transcription and secretion of IL-6 and TNF-alpha were measured using quantitative real time PCR and human cytokine bead-based multiplex immunoassay, respectively. Human THP-1 monocytes were differentiated with phorbol-12-myristate-13-acetate into macrophages which were pre-incubated with physiologically relevant concentrations of blackberry extract or single compounds. Subsequent stimulation with lipopolysaccharides from E.coli induced gene transcription and cytokine release mimicking the inflammatory response. Cyanidin-3-(malonylglucoside) had no impact on LPS-induced IL6 and TNF-alpha expression. In contrast, the blackberry extract and cyanidin-3-(6''-dioxalylglucoside) caused further stimulatory effects for the transcription and secretion of IL-6 at higher concentrations ≥0.05 µg/ml. The secretion of TNF-alpha was increased as well by both, yet with no impact on the transcript level after 3 h of incubation. The results clearly show that single as well as combinatory effects might be of relevance for the immune-stimulatory impact of the blackberry extract., Journal of International Society of Antioxidants in Nutrition & Health, Vol 3, No 4 (2016)

Published
2016

47. Differential phosphodiesterase expression and cytosolic Ca2+ in human CNS tumour cells and in non-malignant and malignant cells of rat origin.

Author

Vatter, Sandra, Pahlke, Gudrun, Deitmer, Joachim W., and Eisenbrand, Gerhard

Subjects
*CYCLIC nucleotide phosphodiesterases, *PHOSPHODIESTERASES, *CANCER cells, *TUMORS, *DRUG therapy, *THERAPEUTICS
Abstract

A promising attempt in the field of tumour therapy is the modulation of intracellular, proliferation-associated signalling pathways. The role of cyclic nucleotide phosphodiesterases (PDEs), key enzymes in cAMP/cGMP signal transduction, was investigated in two human CNS tumour cell lines as well as in the rat glioblastoma cell line C6 in comparison with rat cerebellar astrocytes with the emphasis on target evaluation. We found differential PDEexpression patterns in human CNS tumour cell lines as well as in CNS cells of rat origin. In human glioblastoma cells, intracellular cAMP and Ca2+ levels correlated well with the PDE expression pattern. There were, however, marked differences in PDE expression and Ca2+ kinetics between the human glioblastoma cell lines. In contrast to human epithelial tumour cells, shown earlier by us to express significantly enhanced cAMP-specific PDE activity, this was not the case in rat glioblastoma cells compared with non-malignant rat astrocytes. Despite different levels of PDE1 and PDE4 expression and activity, cyclic nucleotide and Ca2+ levels in non-malignant and malignant rat CNS cells were similar. Thesein vitrodata do not support the concept of PDE1C representing a target exploitable for drug treatment of malignant CNS tumours. [ABSTRACT FROM AUTHOR]

Published
2005
Full Text
View/download PDF

48. Alternaria alternataMycotoxins Activate the Aryl Hydrocarbon Receptor and Nrf2-ARE Pathway to Alter the Structure and Immune Response of Colon Epithelial Cells

Author

Groestlinger, Julia, Spindler, Veronika, Pahlke, Gudrun, Rychlik, Michael, Del Favero, Giorgia, and Marko, Doris

Abstract

After ingestion of food commodities, the gastrointestinal tract (GIT) poses the first barrier against xenobiotics and pathogens. Therefore, it is regularly confronted with external stressors potentially affecting the inflammatory response and the epithelial barrier. Alternariamycotoxins such as alternariol (AOH) and altertoxin II (ATX-II) are frequently occurring food and feed contaminants that are described for their immunomodulatory capacities. Hence, this study aimed at exploring the effect of AOH and ATX-II as single compounds or binary mixtures on the immune response and epithelial homeostasis in noncancerous colon epithelial cells HCEC-1CT. Both toxins suppressed mRNA levels of proinflammatory mediators interleukin-8 (IL-8), tumor necrosis factor α (TNF-α), and secretion of IL-8, as well as mRNA levels of the matrix metallopeptidase 2 (MMP-2). Binary combinations of AOH and ATX-II reduced the response of the single toxins. Additionally, AOH and ATX-II modified immunolocalization of transmembrane proteins such as integrin β1, zona occludens 1 (ZO-1), claudin 4 (Cldn 4), and occludin (Ocln), which support colonic tissue homeostasis and intestinal barrier function. Moreover, the cellular distribution of ZO-1 was affected by ATX-II. Mechanistically, these effects could be traced back to the involvement of several transcription factors. AOH activated the nuclear translocation of the aryl hydrocarbon receptor (AhR) and the nuclear factor erythroid 2-related factor 2 (Nrf2), governing cell metabolic competence and structural integrity. This was accompanied by altered distribution of the NF-κB p65 protein, an important regulator of inflammatory response. ATX-II also induced AhR and Nrf2 translocation, albeit failing to substantiate the effect of AOH on the colonic epithelium. Hence, both toxins coherently repress the intestinal immune response on the cytokine transcriptional and protein levels. Furthermore, both mycotoxins affected the colonic epithelial integrity by altering the cell architecture.

Published
2022
Full Text
View/download PDF

49. Oak ellagitannins suppress the phosphorylation of the epidermal growth factor receptor in human colon carcinoma cells.

Author

Fridrich D, Glabasnia A, Fritz J, Esselen M, Pahlke G, Hofmann T, and Marko D

Subjects
Biphenyl Compounds pharmacology, Catechols pharmacology, Cell Division drug effects, Cell-Free System, Enzyme Inhibitors, Glycosides pharmacology, Glycosylation, HT29 Cells, Heterocyclic Compounds, 4 or More Rings pharmacology, Humans, Phosphorylation drug effects, Protein-Tyrosine Kinases antagonists & inhibitors, ErbB Receptors metabolism, Hydrolyzable Tannins pharmacology, Quercus chemistry
Abstract

The ellagitannins castalagin and vescalagin, and the C-glycosides grandinin and roburin E as well as ellagic acid were found to potently inhibit the growth of human colon carcinoma cells (HT29) in vitro. In a cell-free system these compounds were identified as potent inhibitors of the protein tyrosine kinase activity of the epidermal growth factor receptor (EGFR) with IC 50 values in the low nanomolar range. To address the question of whether the interference with the activity of the isolated EGFR also plays a role within intact cells, effects on the phosphorylation status of the EGFR, as a measure for its activity, were determined in HT29 cells. As exemplified for castalagin and grandinin, both the nonglycosylated and the glycosylated ellagitannins effectively suppressed EGFR phosphorylation, but only at concentrations > or =10 microM, thus, in a concentration range where growth inhibition was observed. These results indicate that the suppression of EGFR-mediated signaling might contribute to the growth inhibitory effects of these compounds present in oak-matured wines and spirits such as whiskey. In contrast, despite substantial growth inhibitory properties, ellagic acid did not significantly affect EGFR phosphorylation in HT29 cells up to 100 microM.

Published
2008
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results