Search

Your search keyword '"Painter, Michio"' showing total 15 results
Start Over Author "Painter, Michio"
15 results on '"Painter, Michio"'

1. Mechanistic Differences in Neuropathic Pain Modalities Revealed by Correlating Behavior with Global Expression Profiling.

Author

Cobos, Enrique J, Nickerson, Chelsea A, Gao, Fuying, Chandran, Vijayendran, Bravo-Caparrós, Inmaculada, González-Cano, Rafael, Riva, Priscilla, Andrews, Nick A, Latremoliere, Alban, Seehus, Corey R, Perazzoli, Gloria, Nieto, Francisco R, Joller, Nicole, Painter, Michio W, Ma, Chi Him Eddie, Omura, Takao, Chesler, Elissa J, Geschwind, Daniel H, Coppola, Giovanni, Rangachari, Manu, Woolf, Clifford J, and Costigan, Michael

Subjects
T-Lymphocytes, Macrophages, Animals, Mice, Inbred C57BL, Mice, Transgenic, Mice, Neuralgia, Hyperalgesia, Behavior, Animal, Touch, Male, TRPV Cation Channels, Cold Temperature, Sensory Receptor Cells, Transcriptome, T cells, TrpV1, WCGNA, cold allodynia, gene expression, immune system, macrophages, neuropathic pain, tactile allodynia, transcript profiling, Inbred C57BL, Transgenic, Behavior, Animal, Pain Research, Chronic Pain, Peripheral Neuropathy, Neurodegenerative, Neurosciences, Clinical Research, 2.1 Biological and endogenous factors, Neurological, Pain Conditions - Chronic, Biochemistry and Cell Biology, Medical Physiology
Abstract

Chronic neuropathic pain is a major morbidity of neural injury, yet its mechanisms are incompletely understood. Hypersensitivity to previously non-noxious stimuli (allodynia) is a common symptom. Here, we demonstrate that the onset of cold hypersensitivity precedes tactile allodynia in a model of partial nerve injury, and this temporal divergence was associated with major differences in global gene expression in innervating dorsal root ganglia. Transcripts whose expression change correlates with the onset of cold allodynia were nociceptor related, whereas those correlating with tactile hypersensitivity were immune cell centric. Ablation of TrpV1 lineage nociceptors resulted in mice that did not acquire cold allodynia but developed normal tactile hypersensitivity, whereas depletion of macrophages or T cells reduced neuropathic tactile allodynia but not cold hypersensitivity. We conclude that neuropathic pain incorporates reactive processes of sensory neurons and immune cells, each leading to distinct forms of hypersensitivity, potentially allowing drug development targeted to each pain type.

Published
2018

2. Enhanced Neuronal Regeneration in the CAST/Ei Mouse Strain Is Linked to Expression of Differentiation Markers after Injury.

Author

Lisi, Véronique, Singh, Bhagat, Giroux, Michel, Guzman, Elmer, Painter, Michio W, Cheng, Yung-Chih, Huebner, Eric, Coppola, Giovanni, Costigan, Michael, Woolf, Clifford J, and Kosik, Kenneth S

Subjects
Neurites, Animals, Mice, MicroRNAs, Nerve Regeneration, Male, Basic Helix-Loop-Helix Transcription Factors, Peripheral Nerve Injuries, Ascl1, CAST/Ei, RNA sequencing, dorsal root ganglia, miR-7048, regeneration, single-cell analyses, Genetics, Injury (total) Accidents/Adverse Effects, Biotechnology, Regenerative Medicine, Neurosciences, 2.1 Biological and endogenous factors, Neurological, Biochemistry and Cell Biology, Medical Physiology
Abstract

Peripheral nerve regeneration after injury requires a broad program of transcriptional changes. We investigated the basis for the enhanced nerve regenerative capacity of the CAST/Ei mouse strain relative to C57BL/6 mice. RNA sequencing of dorsal root ganglia (DRG) showed a CAST/Ei-specific upregulation of Ascl1 after injury. Ascl1 overexpression in DRG neurons of C57BL/6 mice enhanced their neurite outgrowth. Ascl1 is regulated by miR-7048-3p, which is downregulated in CAST/Ei mice. Inhibition of miR-7048-3p enhances neurite outgrowth. Following injury, CAST/Ei neurons largely retained their mature neuronal profile as determined by single-cell RNA- seq, whereas the C57BL/6 neurons acquired an immature profile. These findings suggest that one facet of the enhanced regenerative phenotype is preservation of neuronal identity in response to injury.

Published
2017

3. Robust Axonal Regeneration Occurs in the Injured CAST/Ei Mouse CNS.

Author

Omura, Takao, Omura, Kumiko, Tedeschi, Andrea, Riva, Priscilla, Painter, Michio W, Rojas, Leticia, Martin, Joshua, Lisi, Véronique, Huebner, Eric A, Latremoliere, Alban, Yin, Yuqin, Barrett, Lee B, Singh, Bhagat, Lee, Stella, Crisman, Tom, Gao, Fuying, Li, Songlin, Kapur, Kush, Geschwind, Daniel H, Kosik, Kenneth S, Coppola, Giovanni, He, Zhigang, Carmichael, S Thomas, Benowitz, Larry I, Costigan, Michael, and Woolf, Clifford J

Subjects
Neurosciences, Cognitive Sciences, Neurology & Neurosurgery, Psychology
Published
2016

4. Robust Axonal Regeneration Occurs in the Injured CAST/Ei Mouse CNS.

Author

Omura, Takao, Omura, Kumiko, Tedeschi, Andrea, Riva, Priscilla, Painter, Michio W, Rojas, Leticia, Martin, Joshua, Lisi, Véronique, Huebner, Eric A, Latremoliere, Alban, Yin, Yuqin, Barrett, Lee B, Singh, Bhagat, Lee, Stella, Crisman, Tom, Gao, Fuying, Li, Songlin, Kapur, Kush, Geschwind, Daniel H, Kosik, Kenneth S, Coppola, Giovanni, He, Zhigang, Carmichael, S Thomas, Benowitz, Larry I, Costigan, Michael, and Woolf, Clifford J

Subjects
Ganglia, Spinal, Axons, Animals, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Inbred DBA, Mice, Inbred NOD, Mice, Spinal Cord Injuries, Sciatic Neuropathy, Nerve Regeneration, Mice, 129 Strain, Ganglia, Spinal, Inbred C3H, Inbred C57BL, Inbred DBA, Inbred NOD, Strain, Spinal Cord Injury, Neurosciences, Injury - Trauma -, Genetics, Neurodegenerative, Regenerative Medicine, Brain Disorders, Stem Cell Research - Nonembryonic - Non-Human, Stem Cell Research, Injury (total) Accidents/Adverse Effects, 1.1 Normal biological development and functioning, Neurological, Neurology & Neurosurgery, Cognitive Sciences, Psychology
Abstract

Axon regeneration in the CNS requires reactivating injured neurons' intrinsic growth state and enabling growth in an inhibitory environment. Using an inbred mouse neuronal phenotypic screen, we find that CAST/Ei mouse adult dorsal root ganglion neurons extend axons more on CNS myelin than the other eight strains tested, especially when pre-injured. Injury-primed CAST/Ei neurons also regenerate markedly in the spinal cord and optic nerve more than those from C57BL/6 mice and show greater sprouting following ischemic stroke. Heritability estimates indicate that extended growth in CAST/Ei neurons on myelin is genetically determined, and two whole-genome expression screens yield the Activin transcript Inhba as most correlated with this ability. Inhibition of Activin signaling in CAST/Ei mice diminishes their CNS regenerative capacity, whereas its activation in C57BL/6 animals boosts regeneration. This screen demonstrates that mammalian CNS regeneration can occur and reveals a molecular pathway that contributes to this ability.

Published
2015

6. Reduction of Neuropathic and Inflammatory Pain through Inhibition of the Tetrahydrobiopterin Pathway

Author

Latremoliere, Alban, Latini, Alexandra, Andrews, Nick, Cronin, Shane J., Fujita, Masahide, Gorska, Katarzyna, Hovius, Ruud, Romero, Carla, Chuaiphichai, Surawee, Painter, Michio, Miracca, Giulia, Babaniyi, Olusegun, Remor, Aline Pertile, Duong, Kelly, Riva, Priscilla, Barrett, Lee B., Ferreirós, Nerea, Naylor, Alasdair, Penninger, Josef M., Tegeder, Irmgard, Zhong, Jian, Blagg, Julian, Channon, Keith M., Johnsson, Kai, Costigan, Michael, and Woolf, Clifford J.

Published
2015
Full Text
View/download PDF

8. ImmGen at 15

Author

Aguilar, Stephanie Vargas, Aguilar, Oscar, Allan, Rhys, Amir, El Ad David, Angeli, Veronique, Artyomov, Maxim, Asinovski, Natasha, Astarita, Jilian, Austen, K. Frank, Bajpai, Geetika, Barrett, Nora, Baysoy, Alev, Benoist, Christophe, Bellemare-Pelletier, Angelique, Berg, Brad, Best, Adam, Bezman, Natalie, Blair, David, Blander, Julie, Bogunovic, Milena, Brennan, Patrick, Brenner, Michael, Brown, Brian, Buechler, Matthew, Buenrostro, Jason, Casanova, Maria Acebes, Choi, Kyunghee, Chow, Andrew, Chudnovskiy, Aleksey, Cipoletta, Daniela, Cohen, Nadia, Collins, James, Colonna, Marco, Cook, Alison, Costello, James, Cremasco, Viviana, Crowl, Ty, Crozat, Karine, Cruse, Richard, D’angelo, June, Dalod, Marc, Davis, Scott, Demiralp, Cagatay, Deng, Tianda, Desai, Jigar, Desland, Fiona, Dhainaut, Maxime, Ding, Jiarui, Doedens, Andrew, Dominguez, Claudia, Doran, Graeme, Dress, Regine, Dustin, Michael, Dwyer, Daniel, Dzhagalov, Ivan, Elpek, Kutlu, Ergun, Ayla, Ericson, Jeff, Esomonu, Eunice, Fairfax, Keke, Fletcher, Anne, Frascoli, Michela, Fuchs, Anja, Gainullina, Anastasiia, Gal-Oz, Shani, Gallagher, Michael, Gautier, Emmanuel, Gazit, Roi, Gibbings, Sophie, Giraud, Matthieu, Ginhoux, Florent, Goldrath, Ananda, Gotthardt, Dagmar, Gray, Daniel, Greter, Melanie, Grieshaber-Bouyer, Ricardo, Guilliams, Martin, Haidermota, Sara, Hardy, Randy, Hashimoto, Daigo, Helft, Julie, Hendricks, Deborah, Heng, Tracy, Hill, Jonathan, Hyatt, Gordon, Idoyaga, Juliana, Jakubzick, Claudia, Jarjoura, Jessica, Jepson, Daniel, Jia, Baosen, Jianu, Radu, Johanson, Tim, Jordan, Stefan, Jojic, Vladimir, Kamimura, Yosuke, Kana, Veronica, Kang, Joonsoo, Kapoor, Varun, Kenigsberg, Ephriam, Kent, Andrew, Kim, Charles, Kim, Edy, Kim, Francis, Kim, Joel, Kim, Kiwook, Kiner, Evgeny, Knell, Jamie, Koller, Daphne, Kozinn, Larry, Krchma, Karen, Kreslavsky, Taras, Kronenberg, Mitchell, Kwan, Wing-Hong, Laidlaw, David, Lam, Viola, Lanier, Lewis, Laplace, Catherine, Lareau, Caleb, Lavin, Yonit, Lavine, Kory, Leader, Andrew, Leboeuf, Marylene, Lee, Jacob, Lee, Jisu, Li, Bo, Li, Hu, Li, Yuesheng, Lionakis, Michail, Luche, Herve, Lynch, Lydia, Magen, Assaf, Maier, Barbara, Malhotra, Deepali, Malhotra, Nidhi, Malissen, Marie, Maslova, Alexandra, Mathis, Diane, Mcfarland, Adelle, Merad, Miriam, Meunier, Etienne, Miller, Jennifer, Milner, Justin, Mingueneau, Michael, Min-Oo, Gundula, Monach, Paul, Moodley, Devapregasan, Mortha, Arthur, Morvan, Maelig, Mostafavi, Sara, Muller, Soren, Muus, Christoph, Nabekura, Tsukasa, Rao, Tata Nageswara, Narang, Vipin, Narayan, Kavitha, Ner-Gaon, Hadas, Nguyen, Quyhn, Nigrovic, Peter, Novakovsky, German, Nutt, Stephan, Omilusik, Kayla, Ortiz-Lopez, Adriana, Paidassi, Helena, Paik, Henry, Painter, Michio, Paynich, Mallory, Peng, Vincent, Potempa, Marc, Pradhan, Rachana, Price, Jeremy, Qi, Yilin, Qi, Yiqing, Quon, Sara, Ramirez, Ricardo, Ramanan, Deepshika, Randolph, Gwendalyn, Regev, Aviv, Rhoads, Andrew, Robinette, Michelle, Rose, Samuel, Rossi, Derrick, Rothamel, Katie, Sachidanandam, Ravi, Sathe, Priyanka, Scott, Charlotte, Seddu, Kumba, See, Peter, Sergushichev, Alexey, Shaw, Laura, Shay, Tal, Shemesh, Avishai, Shinton, Susan, Shyer, Justin, Sieweke, Michael, Smillie, Chris, Spel, Lotte, Spidale, Nick, Stifano, Giuseppina, Subramanian, Ayshwarya, Sun, Joseph, Sylvia, Katelyn, Tellier, Julie, This, Sébastien, Tomasello, Elena, Todorov, Helena, Turley, Shannon, Vijaykumar, Brinda, Wagers, Amy, Wakamatsu, Ei, Wang, Chendi, Wang, Peter, Wroblewska, Aleksandra, Wu, Jun, Yang, Edward, Yang, Liang, Yim, Aldrin, Yng, Lim Sheau, Yoshida, Hideyuki, Yu, Bingfei, Zhou, Yan, Zhu, Yanan, Ziemkiewicz, Caroline, Microenvironment, Cell Differentiation, Immunology and Cancer (MICMAC), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), The Walter and Eliza Hall Institute of Medical Research (WEHI), Icahn School of Medicine at Mount Sinai [New York] (MSSM), National University of Singapore (NUS), Washington University School of Medicine in St. Louis, Washington University in Saint Louis (WUSTL), Harvard Medical School [Boston] (HMS), Genentech, Inc. [San Francisco], Brigham & Women’s Hospital [Boston] (BWH), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Brown University, Lymphocytes B effecteurs et à mémoire – Effector and memory B cells, Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Technische Universität Dresden = Dresden University of Technology (TU Dresden), Crozat, Karine, and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL)

Subjects
0301 basic medicine, Regulation of gene expression, [SDV.IMM] Life Sciences [q-bio]/Immunology, Test data generation, Computer science, [SDV]Life Sciences [q-bio], Immunology, Gene regulatory network, Genomics, Genome project, Data science, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, microRNA, Immunology and Allergy, DECIPHER, [SDV.IMM]Life Sciences [q-bio]/Immunology, Scientific publishing, ComputingMilieux_MISCELLANEOUS, 030215 immunology
Abstract

International audience; 700 comment | SERIES ImmGen at 15 Nature Immunology's 20 th anniversary is a good opportunity to reminisce about the ImmGen collective endeavor-its goals, successes and horror stories-and the group's exploration of various modes of scientific publishing. The Immunological Genome Project T he Immunological Genome Project (ImmGen) is a collaborative group of immunology and computational biology laboratories that perform a thorough dissection of gene expression and its regulation in the immune system of the mouse. This activity first centered on mRNA expression and then expanded to microRNA (miRNA), chromatin structure, nuclear organization and protein-RNA relationships. Shared protocols, data generation and QC pipelines have yielded data that can be directly compared from >250 stem, lymphoid and myeloid cell types, at baseline or under challenge. The group develops and applies computational tools to decipher regulatory connections and transcriptional control. From its inception, data generated by ImmGen were meant to be a public resource, and they can be accessed through dedicated web and smartphone platforms that use interactive graphic displays that make the results intuitive to users.

Published
2020
Full Text
View/download PDF

9. Transcriptomes of the B and T Lineages Compared by Multiplatform Microarray Profiling

Author

Painter, Michio W., Davis, Scott, Hardy, Richard R., Mathis, Diane, Benoist, Christophe, Immunological Genome Project Consortium, Massachusetts Institute of Technology. Department of Biology, and Regev, Aviv

Subjects
Gene expression profiling, Transcriptome, Genetics, Cell type, Immune system, Cellular differentiation, Immunology, Immunology and Allergy, Biology, Acquired immune system, Gene, Genome
Abstract

T and B lymphocytes are developmentally and functionally related cells of the immune system, representing the two major branches of adaptive immunity. Although originating from a common precursor, they play very different roles: T cells contribute to and drive cell-mediated immunity, whereas B cells secrete Abs. Because of their functional importance and well-characterized differentiation pathways, T and B lymphocytes are ideal cell types with which to understand how functional differences are encoded at the transcriptional level. Although there has been a great deal of interest in defining regulatory factors that distinguish T and B cells, a truly genomewide view of the transcriptional differences between these two cells types has not yet been taken. To obtain a more global perspective of the transcriptional differences underlying T and B cells, we exploited the statistical power of combinatorial profiling on different microarray platforms, and the breadth of the Immunological Genome Project gene expression database, to generate robust differential signatures. We find that differential expression in T and B cells is pervasive, with the majority of transcripts showing statistically significant differences. These distinguishing characteristics are acquired gradually, through all stages of B and T differentiation. In contrast, very few T versus B signature genes are uniquely expressed in these lineages, but are shared throughout immune cells., National Institute of Allergy and Infectious Diseases (U.S.) (National Institutes of Health (R24 AI072073)

Published
2011
Full Text
View/download PDF

10. Enhanced neuronal regeneration in the CAST/Ei mouse strain is linked to expression of differentiation markers after injury

Author

Lisi, Véronique, primary, Singh, Bhagat, additional, Giroux, Michel, additional, Guzman, Elmer, additional, Painter, Michio W, additional, Cheng, Yung-Chih, additional, Huebner, Eric, additional, Coppola, Giovanni, additional, Costigan, Michael, additional, Woolf, Clifford J., additional, and Kosik, Kenneth S, additional

Published
2017
Full Text
View/download PDF

11. Robust axonal regeneration occurs in the injured CAST/Ei mouse central nervous system

Author

Omura, Takao, Omura, Kumiko, Tedeschi, Andrea, Riva, Priscilla, Painter, Michio W, Rojas, Leticia, Martin, Joshua, Lisi, Véronique, Huebner, Eric A, Latremoliere, Alban, Yin, Yuqin, Barrett, Lee, Singh, Bhagat, Lee, Stella, Crisman, Tom, Gao, Fuying, Li, Songlin, Kapur, Kush, Geschwind, Daniel H, Kosik, Kenneth S, Coppola, Giovanni, He, Zhigang, Carmichael, S Thomas, Benowitz, Larry I, Costigan, Michael, and Woolf, Clifford J

Subjects
Mice, Inbred C3H, Mice, 129 Strain, Article, Axons, Nerve Regeneration, Mice, Inbred C57BL, Mice, nervous system, Mice, Inbred DBA, Mice, Inbred NOD, Ganglia, Spinal, Animals, Sciatic Neuropathy, Spinal Cord Injuries
Abstract

Axon regeneration in the central nervous system (CNS) requires reactivating injured neurons’ intrinsic growth state and enabling growth in an inhibitory environment. Using an inbred mouse neuronal phenotypic screen, we find that CAST/Ei mouse adult dorsal root ganglion neurons extend axons more on CNS myelin than the other eight strains tested, especially when pre-injured. Injury-primed CAST/Ei neurons also regenerate markedly in the spinal cord and optic nerve more than those from C57BL/6 mice and show greater spouting following ischemic stroke. Heritability estimates indicate that extended growth in CAST/Ei neurons on myelin is genetically determined, and two whole-genome expression screens yield the Activin transcript Inhba as most correlated with this ability. Inhibition of Activin signaling in CAST/Ei mice diminishes their CNS regenerative capacity whereas its activation in C57BL/6 animals boosts regeneration. This screen demonstrates that mammalian CNS regeneration can occur and reveals a molecular pathway that contributes to this ability.

Published
2015

12. Transcriptomes of the B and T lineages compared by multi-platform microarray profiling1

Author

Painter, Michio W., Davis, Scott, Hardy, Richard R., Mathis, Diane, and Benoist, Christophe

Subjects
Genome, Transcription, Genetic, Gene Expression Profiling, B-Lymphocyte Subsets, Cell Differentiation, Article, High-Throughput Screening Assays, Mice, Inbred C57BL, Mice, Random Allocation, T-Lymphocyte Subsets, Consensus Sequence, Animals, Cell Lineage, Oligonucleotide Array Sequence Analysis, Signal Transduction
Abstract

T and B lymphocytes are developmentally and functionally related cells of the immune system, representing the two major branches of adaptive immunity. Although originating from a common precursor, they play very different roles: T cells contribute to and drive cell-mediated immunity, while B cells secrete antibodies. Because of their functional importance and well-characterized differentiation pathways, T and B lymphocytes are ideal cell-types with which to understand how functional differences are encoded at the transcriptional level. Although there has been a great deal of interest in defining regulatory factors that distinguish T and B cells, a truly genome-wide view of the transcriptional differences between these two cells types has not yet been taken. To obtain a more global perspective of the transcriptional differences underlying T and B cells, we exploited the statistical power of combinatorial profiling on different microarray platforms, and the breadth of the Immunological Genome Project (ImmGen) gene-expression database, to generate robust differential signatures. We find that differential expression in T and B cells is pervasive, the majority of transcripts showing statistically significant differences. These distinguishing characteristics are acquired gradually, though all stages of B and T differentiation. On the other hand, very few T vs. B signature genes are uniquely expressed in these lineages, but are shared throughout immune cells.

Published
2011

13. Transcriptomes of the B and T lineages compared by multiplatform microarray profiling

Author

Massachusetts Institute of Technology. Department of Biology, Regev, Aviv, Painter, Michio W., Davis, Scott, Hardy, Richard R., Mathis, Diane, Benoist, Christophe, Immunological Genome Project Consortium, Massachusetts Institute of Technology. Department of Biology, Regev, Aviv, Painter, Michio W., Davis, Scott, Hardy, Richard R., Mathis, Diane, Benoist, Christophe, and Immunological Genome Project Consortium

Abstract

T and B lymphocytes are developmentally and functionally related cells of the immune system, representing the two major branches of adaptive immunity. Although originating from a common precursor, they play very different roles: T cells contribute to and drive cell-mediated immunity, whereas B cells secrete Abs. Because of their functional importance and well-characterized differentiation pathways, T and B lymphocytes are ideal cell types with which to understand how functional differences are encoded at the transcriptional level. Although there has been a great deal of interest in defining regulatory factors that distinguish T and B cells, a truly genomewide view of the transcriptional differences between these two cells types has not yet been taken. To obtain a more global perspective of the transcriptional differences underlying T and B cells, we exploited the statistical power of combinatorial profiling on different microarray platforms, and the breadth of the Immunological Genome Project gene expression database, to generate robust differential signatures. We find that differential expression in T and B cells is pervasive, with the majority of transcripts showing statistically significant differences. These distinguishing characteristics are acquired gradually, through all stages of B and T differentiation. In contrast, very few T versus B signature genes are uniquely expressed in these lineages, but are shared throughout immune cells., National Institute of Allergy and Infectious Diseases (U.S.) (National Institutes of Health (R24 AI072073)

Published
2013

14. The Immunological Genome Project: networks of gene expression in immune cells.

Author

Heng, Tracy S P, Painter, Michio W, Elpek, Kutlu, Lukacs-Kornek, Veronika, Mauermann, Nora, Turley, Shannon J, Koller, Daphne, Kim, Francis S, Wagers, Amy J, Asinovski, Natasha, Davis, Scott, Fassett, Marlys, Feuerer, Markus, Gray, Daniel H D, Haxhinasto, Sokol, Hill, Jonathan A, Hyatt, Gordon, Laplace, Catherine, Leatherbee, Kristen, and Mathis, Diane

Abstract

The Immunological Genome Project combines immunology and computational biology laboratories in an effort to establish a complete 'road map' of gene-expression and regulatory networks in all immune cells [ABSTRACT FROM AUTHOR]

Published
2008
Full Text
View/download PDF

15. Regeneration in the aging peripheral nervous system

Author

Painter, Michio Wendell, Woolf, Clifford, and Benowitz, Larry Ira

Subjects
Neurosciences
Abstract

In the peripheral nervous system (PNS), aging is associated with a number of disorders, including a decline in regenerative capacity after injury. Although this decline has been observed in both rodents and humans for decades, the cellular and molecular underpinnings of this defect have remained elusive. As such, the goal of this thesis was to elucidate, at least in part, how aging impinges on axonal regeneration.

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results