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The proliferation of pest and invasive marine macroalgae threatens coastal ecosystems, with biotic interactions, including direct effects such as grazing and indirect effects such as the trophic cascades, where one species indirectly affects another through its interactions with a third species, play a critical role in determining the resistance of local communities to these invasions. This study examines the foraging behaviour and preference of native fish communities toward native (Halopteris scoparia, Sargassum vulgare) and non-indigenous (Asparagopsis taxiformis) macroalgae using the Remote Video Foraging System (RVFS). Fifty-four weedpops were deployed across three locations to present these macroalgae, while associated epifaunal assemblages were also collected. Video analysis revealed that four common fish species displayed preference towards native macroalgae, possibly due to by the presence of zoobenthos rather than herbivory. This observation suggests that these fish species identified the macroalgae as a habitat that harboured their preferred food items. In contrast, A. taxiformis was consistently avoided, suggesting limited integration into the local food web. Site-specific variations in fish-macroalgae interactions and epifaunal diversity highlighted the complexity of these dynamics. This study contributes to understanding of the ecological implications of invasive macroalgae and supports the use of RVFS as a tool for assessing local biotic resistance against non-indigenous species in coastal ecosystems globally., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Non-indigenous species (NIS) spread from marinas to natural environments is influenced by niche availability, habitat suitability, and local biotic resistance. This study explores the effect of indigenous fish feeding behaviour on NIS proliferation using fouling communities, pre-grown on settlement plates, as two distinct, representative models: one from NIS-rich marinas and the other from areas outside marinas with fewer NIS. These plates were mounted on a Remote Video Foraging System (RVFS) near three marinas on Madeira Island. After 24-h, NIS abundance was reduced by 3.5 %. Canthigaster capistrata's preference for marinas plates suggests potential biotic resistance. However, Sparisoma cretense showed equal biting frequencies for both plate types. The cryptogenic ascidian Trididemnum cereum was the preferred target for the fish. Our study introduces a global framework using RVFS for in-situ experiments, replicable across divers contexts (e.g., feeding behaviour, biotic resistance), which can be complemented by metabarcoding and isotopic analysis to confirm consumption patterns., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Introduction Acetylsalicylic acid (ASA) has commonly been touted for its potential to extend the aesthetic lifespan (EL) of Christmas trees when added to the water in the tree stand. This study examined the efficacy of ASA in prolonging the aesthetic longevity of spruce branches, in comparison to placebo and sildenafil. Intervention We conducted a triple-blinded, randomised clinical trial, wherein 60 spruce branches were allocated to one of three treatment arms in a 1:1:1 ratio. The primary intervention was ASA, compared against both placebo and sildenafil treatments. The study's primary endpoint was the EL of the spruce branches. Results All participating branches completed the study. No statistically significant differences were observed in the survival times across the three groups: ASA 17 days (standard deviation (SD): 6), placebo 20 days (SD: 8), and sildenafil 21 days (SD: 7); p = 0.30. Both the log-rank test and adjusted Cox proportional-hazards analyses failed to show any significant variations in aesthetic survival time among the treatment arms (p > 0.05). Conclusion Given our findings, there is no empirical support for the widely held recommendation of adding ASA to the water at the base of a Christmas tree to extend its aesthetic lifespan. Funding none. Trial registration none., (Published under Open Access CC-BY-NC-BD 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/.)

This paper presents a clinical case study investigating the pattern of a saxophonist's embouchure as a possible origin of orofacial pain. The rehabilitation addressed the dental occlusion and a fracture in a metal ceramic bridge. To evaluate the undesirable loads on the upper teeth, two piezoresistive sensors were placed between the central incisors and the mouthpiece during the embouchure. A newly fixed metal ceramic prosthesis was placed from teeth 13 to 25, and two implants were placed in the premolar zone corresponding to teeth 14 and 15. After the oral rehabilitation, the embouchure force measurements showed that higher stability was promoted by the newly fixed metal-ceramic prosthesis. The musician executed a more symmetric loading of the central incisors (teeth 11 and 21). The functional demands of the saxophone player and consequent application of excessive pressure can significantly influence and modify the metal-ceramic position on the anterior zone teeth 21/22. The contribution of engineering (i.e., monitoring the applied forces on the musician's dental structures) was therefore crucial for the correct assessment and design of the treatment plan.

Microplastics (<5 mm) are a ubiquitous marine pollutant which are highly bioavailable to marine organisms across all trophic levels. Marine predators are especially vulnerable to microplastic pollution through direct and indirect ingestion (e.g., trophic transfer) due to their high trophic position. In particular, oceanic islands are more susceptible to plastic accumulation, increasing the relative number of microplastics in the environment that are available for consumption. The dynamics of microplastic accumulation in marine predators inhabiting remote islands, however, is sparsely documented. Here we describe microplastic exposure in the Critically Endangered Mediterranean monk seal (Monachus monachus) from the Madeira archipelago (Northeast Atlantic) using scat-based analysis. Microplastics were recovered from 18 scat samples collected between 2014-2021 and were characterized to the polymer level using Fourier-Transform Infrared (u-FTIR) spectroscopy. A total of 390 microplastic particles were recovered, ranging between 0.2-8.6 particles g -1 dry weight (mean 1.84 ± 2.14 particles g -1 ) consisting mainly of fragments (69 %) of various sizes and polymer composition (e.g., PE, PET, PS). Microplastic prevalence (100 % of samples analysed) was higher than what has been previously recorded using scat-based analysis in other pinniped species. Our results suggest that the levels of microplastic pollution in the coastal food-web in the Madeira archipelago are relatively high, placing higher-trophic level organisms at increased risk of microplastic consumption, including humans. This study provides the first insights into microplastic exposure to Madeira's monk seals that may contribute to future management decisions for the species and their long-term survival., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Multi-trait genomic prediction (MTGP) can improve selection accuracy for economically valuable 'primary' traits by incorporating data on correlated secondary traits. Resistance to Fusarium head blight (FHB), a fungal disease of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.), is evaluated using four genetically correlated traits: incidence (INC), severity (SEV), Fusarium damaged kernels (FDK), and deoxynivalenol content (DON). Both FDK and DON are primary traits; DON evaluation is expensive and usually requires several months for wheat breeders to get results from service laboratories performing the evaluations. We evaluated MTGP for DON using three soft red winter wheat breeding datasets: two diversity panels from the University of Illinois (IL) and Purdue University (PU) and a dataset consisting of 2019-2020 University of Illinois breeding cohorts. For DON, relative to single-trait (ST) genomic prediction, MTGP including phenotypic data for secondary traits on both validation and training sets, resulted in 23.4 and 10.6% higher predictive abilities in IL and PU panels, respectively. The MTGP models were advantageous only when secondary traits were included in both training and validation sets. In addition, MTGP models were more accurate than ST models only when FDK was included, and once FDK was included in the model, adding additional traits hardly improved accuracy. Evaluation of MTGP models across testing cohorts indicated that MTGP could increase accuracy by more than twofold in the early stages. Overall, we show that MTGP can increase selection accuracy for resistance to DON accumulation in wheat provided FDK is evaluated on the selection candidates., (© 2022 The Authors. The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America.)

6-Shogaol is one of the main active phenolic components of ginger and has neuroprotective effects by protecting brain against the oxidative stress and regulate the levels of neurotrophic factors. The objective of the present study was to verify the effect of 6-shogaol on neurochemical parameters in offspring after maternal immune activation by lipopolysaccharide (LPS) in rats. Twelve pregnant Wistar rats received 100 μg/kg of LPS or saline solution on the gestational day 9.5. Male offspring participated in the study and from the postnatal days (PND) 30 and 55, respectively, they were supplemented with 6-shogaol or saline solution, by gavage at a dose of 10 mg/kg/day, orally for 5 days. In PND 37 and 62, analysis of kinase signaling regulated by extracellular signal 1/2 (ERK 1/2), levels of neurotrophic factor derived from the brain (BDNF), and neuron-specific enolase (NSE), lipid and protein oxidative damage was evaluated by 4-hydroxy-2-nonenal (HNE) and 3-nitrotyrosine (3-NT), respectively, and myeloperoxidase (MPO) activity was performed in the hippocampus. Prenatal exposure to LPS significantly decreased ERK and BDNF levels in PND 37 and 62, increased NSE levels and lipid damage in rats in PND 37, and increased 3-NT level in rats in PND 62. With treatment using 6-shogaol, an increase in ERK and BDNF levels was identified in PND 37 and 62 and a reduction in HNE and MPO activity in rats in PND 37 and 62, respectively. 6-Shogaol positively increased markers of neuronal growth, plasticity and synaptic activity and reduced oxidative damage in the hippocampus in an animal model of autism by maternal immune activation., (© 2021 S. Karger AG, Basel.)

Background: Specific dentofacial characteristics in wind instrumentalists should be taken in consideration when analyzing physiological and anatomical issues regarding the musician's embouchure, posture, and biomechanics during musical performance., Objectives: To compare tooth cephalometric characteristics between wind instrument players and string players (overjet, overbite, lower facial height, facial convexity, lower incisor inclination, and interincisal angle)., Methods: In total, 48 wind instrumentalists (67%) and 24 string instrumentalists (33%). These musicians performed lateral tele-radiography and the correspondent linear and angular measurements of the dentofacial cephalometric analysis. Statistical comparison of wind and string instrumentalists was made by using an independent t-test., Results: Small variations on the analyzed parameters were found between the wind and string instrument groups. Based on the cephalometric analysis the variable interincisal angle was statistically significant ( p < 0.05), when comparing the wind and string instrument group., Conclusions: Knowledge of the overjet and overbite value permits a substantial analysis on the tooth position of wind instrument players, where both of these parameters are increased and greater than the norm value. The cephalometry was an added value on the interpretation of possible factors that lead to the position of the central incisors of wind instruments. Till some extent in this group of musicians the applied forces during the embouchure mechanism on the anterior teeth and the existing perioral forces promote an equilibrium on the vector of forces. This study findings demonstrate that when evaluating the two samples, wind and string instruments there are different dentofacial configurations, however the only statistically significant differences that were found are related to the interincisal angle ( p < 0.05).

The present work suggests research and innovation on the topic of dental education after the COVID-19 pandemic, is highly justified and could lead to a step change in dental practice. The challenge for the future in dentistry education should be revised with the COVID-19 and the possibility for future pandemics, since in most countries dental students stopped attending the dental faculties as there was a general lockdown of the population. The dental teaching has an important curriculum in the clinic where patients attend general dentistry practice. However, with SARS-CoV-2 virus, people may be reluctant having a dental treatment were airborne transmission can occur in some dental procedures. In preclinical dental education, the acquisition of clinical, technical skills, and the transfer of these skills to the clinic are extremely important. Therefore, dental education has to adapt the curriculum to embrace new technology devices, instrumentations systems, haptic systems, simulation based training, 3D printer machines, to permit validation and calibration of the technical skills of dental students.

Background: The occurrence of an orofacial trauma can originate health, social, economic and professional problems. A 13-year boy suffered the avulsion of tooth 11 and 21, lost at the scenario., Methods: Three intraoral appliances were manufactured: A Hawley appliance with a central expansion screw and two central incisors (1), trumpet edentulous anterior tooth appliance (2) and a customized splint (3) were designed as part of the rehabilitation procedure. Objectively assessing the sound quality of the trumpet player with these new devices in terms of its spectral, temporal, and spectro-temporal audio properties. A linear frequency response microphone was adopted for precision measurement of pitch, loudness, and timbre descriptors., Results: Pitch deviations may result from the different intra-oral appliances due to the alteration of the mouth cavity, respectively, the area occupied and modification/interaction with the anatomy. This investigation supports the findings that the intra-oral appliance which occupies less volume is the best solution in terms of sound quality., Conclusions: Young wind instrumentalists should have dental impressions of their teeth made, so their dentist has the most reliable anatomy of the natural teeth in case of an orofacial trauma. Likewise, the registration of their sound quality should be done regularly to have standard parameters for comparison.

Purpose: Musculoskeletal disorders can be common in wind and string instrumentalists. The musical gesture technique associated to musical performance can promote an asymmetrical isometric contraction with the hyperactivity of specific muscular groups and the overload of articular surfaces which can cause discomfort or pain. The aim of this investigation was to assess specific anatomical regions of interest within the cranio-cervico-mandibular complex in order to determine and evaluate the presence of muscular hyperactivity associated to musical performance., Methods: An infrared imaging camera, FLIR®, was used to record the regions of interest in wind (n = 48) and string instrumentalists (n = 29). Bilateral temperature differences were considered as thermal asymmetries with a conventional threshold of 0.3 ºC to distinguish pathological from healthy states. The regions of interest (ROI) were the anterior triangle of the neck, the sternocleidomastoid and trapezius muscles regarding the postural and stabilizing muscles of the head and neck. On the orofacial region, the anatomical sites were the anterior temporal muscle, the masseter muscle and the temporomandibular joint. A Pearson chi-square test was performed to compare the assessed areas., Results: Between 30-37% of all participants showed ROI in an asymmetric pattern. The most affected sites were the temporal muscle and the TMJ for both groups. The anterior triangle of the neck showed statistical differences (p = 0.044) between string and wind instrumentalists, while the temporal (p = 0.034) and trapezius muscle (p = 0.028) when comparing large and small mouthpieces of brass instruments. Although female participants showed a higher prevalence of asymmetrical patterns regarding the ROI, no statistical differences were found between genders., Conclusions: Infrared thermography exhibited significant differences between wind and string instruments within the CCMC. Regarding the issue of occupational health in performing arts, it is an advantage being able to quantify asymmetrical patterns sites of the CCMC to understand the underlying physiological responses to repetitive movements, overloading and muscular hyperactivity that occur during musical performance.

Background: Playing an instrument may promote a parafunctional behavior within the cranio-cervical-mandibular-complex with unknown repercussions. The aim of this study was to find any association between the dental inter-arch relationship and the practice of a wind or string instrument., Methods: A sample of 77 musicians, divided in two groups of wind (n = 50) and string instrumentalists (n = 27), had a lateral cephalogram taken to compare six cephalometric parameters following the Rickett's analysis (maxilla position, mandible position, facial type, skeletal class, upper incisor and lower incisor inclination). The Fisher test was performed to compare, with a 95% statistical confidence, if both groups have similar frequency distributions for each cephalometric parameter., Results: No statistical differences were found for the maxilla position, mandible position, facial type, skeletal class and upper incisor inclination. Statistical differences were found for the lower incisor inclination (p = 0.011)., Conclusions: Playing a wind instrument showed to have little orthopaedic influence at the craniofacial morphology, on contrary it may influence the lower incisor inclination with its osseous base.

Antidepressants such as fluoxetine are frequently detected in estuaries and can have profound effects on non-target organisms by interfering with the neural system and affecting essential physiological processes and behaviours. In this context, short-term effects of fluoxetine exposure were analysed in the common goby Pomatoschistus microps, an estuarine resident fish species. Two experiments were conducted with fish exposed to: i) fluoxetine concentrations within the μg/L range for 96 h (0.1, 0.5, 10 and 100 μg/L) and ii) fluoxetine concentrations within the mg/L range for 1 h (1, 5 and 10 mg/L). Acute toxicity was assessed via multiple biomarker responses, namely: activity levels of antioxidant (superoxide dismutase and catalase) and detoxification enzymes (ethoxyresorufin O-deethylase and glutathione S-transferase); and biomarkers of effects (lipid peroxidation and DNA damage) and of neurotoxicity (acetylcholinesterase inhibition). Furthermore, behavioural responses concerning activity (active time, movement delay and number of active individuals) and feeding (number of feeding individuals) were also recorded and analysed. Acute fluoxetine exposure for 96 h (in the μg/L range) reduced antioxidant CAT activity with increasing concentrations but had no significant effect on SOD activity. Biotransformation enzymes showed bell-shaped response curves, suggesting efficient fluoxetine metabolism at concentrations up to 10 μg/L. No significant damage (LPO and DNAd) was observed at both concentration ranges (μg/L and mg/L), yet 1 h exposure to higher fluoxetine concentrations (mg/L range) inhibited acetylcholinesterase activity (up to 37%). Fluoxetine (at mg/L) also decreased the number of both feeding and active individuals (by 67%), decreased fish active time (up to 93%) and increased movement delay almost 3-fold (274%). Overall, acutely exposed P. microps were able to cope with fluoxetine toxicity at the μg/L range but higher concentrations (mg/L) affected fish cholinergic system and behavioural responses., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Introduction: Temporomandibular disorders (TMD) involve the presence of pain or dysfunction on certain areas of the Cranio-Cervico-Mandibular Complex (CCMC), such as the masticatory muscles, the temporomandibular joint (TMJ) and associated structures like the postural muscles of the cervical region, can be considered as a sub-group of musculoskeletal disorders. Wind instrument players, as a consequence of their musical performance and its relation with the CCMC, can develop a TMD associated to muscle hyperactivity of certain elevator muscles, or even an increase of the intra-articular pressure in the functioning of the TMJ throughout musical activity., Aim: The objective of this paper is to describe the necessary and elementary steps in the diagnoses and treatment of a wind instrumentalist with a temporomandibular disorder, with the introduction of infrared thermography during this procedure. This case study also has the purpose of presenting the usefulness of piezoresistive sensors in the analysis of the clarinettists' embouchure., Methodology: A Caucasian, 30-year-old female clarinettist was assessed through a clinical examination following the Diagnostic Criteria for TMD (RDC/TMD), as a complementary tool of diagnosis, a thermal imaging infrared camera, Flir E60 (Wilsonville, OR, USA), was used in order to analyse the above referred articular and muscular regions. The complementary examination protocol implemented with this clarinet player also involved the analyses of the embouchure with the support of piezoresistive sensors., Results: The clinical outcomes resulting from this work were based on the RDC/TMD diagnoses indicated that the clarinet player had an internal derangement on both TMJ, with an osteoarthritis on the left TMJ and an anterior disc displacement with reduction on the right TMJ. The infrared thermograms that were analysed, verified the existence of a temperature differential of the anterior temporal muscle (0.1 °C), the TMJ (0.1 °C) and the masseter muscle (0.7 °C), and after the occlusal splint therapy the asymmetry related to the master muscle reduced to 0.3 °C. The high pitches can reach values of 379 g of force induced to the tooth 21 comparing to the 88 g of force applied on tooth 11. The embouchure force measurements consistently presented greater forces during the higher notes, followed by the medium notes and finally the low notes and this happened with higher pressures being transmitted always to tooth 21., Conclusion: Performing arts medicine should understand the major importance of the dentistry field in the daily life of a professional musician, and the significance of implementing routine screening procedures of dental examinations, with infrared thermograms examination of distinct areas of the CCMC, as well as the use of sensors on the analyses of an eventual asymmetrical embouchure. Employing these techniques in dentistry will create the chance of preventing the overuse of some anatomical structures, with an early diagnosis and the correct monitoring of these areas.

Bias in underwater visual census has always been elusive. In fact, the choice of sampling method and the behavioural traits of fish are two of the most important factors affecting bias, but they are still treated separately, which leads to arbitrarily chosen sampling methods. FishCensus, a two-dimensional agent-based model with realistic fish movement, was used to simulate problematic behavioural traits in SCUBA diving visual census methods and understand how sampling methodology affects the precision and bias of counts. Using a fixed true density of 0.3 fish/m 2 and a fixed visibility of 6 m, 10 counts were simulated for several combinations of parameters for transects (length, width, speed) and point counts (radius, rotation speed, time), generating trait-specific heatmaps for bias and precision. In general, point counts had higher bias and were less precise than transects. Fish attracted to divers led to the highest bias, while cryptic fish had the most accurate counts. For point counts, increasing survey time increased bias and variability, increasing radius reduced bias for most traits but increased bias in the case of fish that avoid divers. Rotation speed did not have a significant effect in general, but it increased bias for fish that avoid divers. Wider and longer transects and a faster swim speed are beneficial when sampling mobile species, but a narrower, shorter transect with a slow swim is beneficial for cryptic fish., Competing Interests: The authors declare there are no competing interests.

The importance of establishing the ecological quality of estuarine systems has been widely acknowledged and led to the development of several fish community-based multimetric indices. Nevertheless, a question rose about the accuracy of these tools when natural disturbance is acting upon the organization of the systems' communities. Four multimetric indices were used to examine their ability to differentiate the ecological status of five small estuarine systems (southern Portugal), and also to test if they reflected the level of anthropogenic pressures. Fish assemblages from Mira, Odeceixe and Aljezur (in the Southwest coast), Gilão and Bensafrim (in the South coast) estuaries were sampled seasonally for one year, and anthropogenic sources of pressure were identified and quantified. We found that although the applied indices provided information on ecological condition differentiation among systems, they are unable to explain different classes of ecological status in systems with equivalent pressure levels., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

The use of arthropods for monitoring habitat changes has grown widely in the last decades. In Brazil, however, most of the studies in restored areas have involved only vegetation changes. The present study aimed at investigating recolonization patterns of epigeic arthropods in recently restored sites of semideciduous forests in southeastern Brazil. We compared the community structure of adjoining sites 5, 17, 29 and 36 months old with that at a nearby forest remnant (reference site). We also determined the most abundant species and looked for ecological indicator species of each site age. Arthropods were sampled using pitfall traps, and their assemblages were described and compared with multi- and univariate statistical methods. Species abundance and richness equivalent to the reference site were reached at five months after planting, however species composition was very distinctive not only in relation to the reference site, but also among restored sites. Some of the main species found in this restoration stage are common in agroecosystems or cerrado vegetation. Nevertheless, there was a clear trend of arthropod fauna in restored sites moving toward the fauna in the forest remnant over time. Our results also highlighted ants and termites because of their abundance and ants because of their high value as ecological indicators of restoration age.

Objective: Phenotypical Down syndrome includes pharyngeal and maxillary hypoplasia and, frequently, constricted maxillary arch with nasal obstruction., Study Design: This clinical trial assessed the effects of rapid maxillary expansion on ENT disorders in 24 children with Down syndrome randomly allocated to receive either rapid maxillary expansion or not. Each group received ENT and speech therapy assessments before expansion and after the device had been removed., Results: In the rapid maxillary expansion group, the yearly ENT infection rate was reduced when assessed after device removal (p < 0.01). The parents of rapid maxillary expansion children reported a reduction in respiratory obstruction symptoms. Audiological assessment revealed improvements in the rapid maxillary expansion group (p < 0.01). Cephalometry showed increased maxillary width in the rapid maxillary expansion group., Conclusions: Rapid maxillary expansion resulted in a reduction in hearing loss, yearly rate of ENT infections and parentally assessed symptoms of upper airway obstruction, compared with no treatment. These findings are probably related to expanded oronasal space, due to rapid maxillary expansion.

In this paper the Marine Fish Community Index (MFCI) for the assessment of ecological status of marine environment is proposed. The MFCI was divided into 4 typologies: Rocky subtidal; shallow, intermediate and deep soft-bottoms. Based on the typical community associated to each typology and the DPSIR analysis performed, a set of metrics were selected and tested through a multiple correlation matrix (Pearson's coefficient) and the core ones included in the index. The MFCI was applied in all typologies and the scores obtained with each metric were analyzed. In order to test the robustness of the MFCI the final ecological value of each zone was recalculated by removing successively one metric at a time. The MFCI showed a sensitive and robust response in the ecological status assessment. Since it incorporates both functional and structural community information, the MFCI can be useful in the context of the Marine Strategy Framework Directive as well as in other contexts of conservation and sustainable management of the marine environment.

The assessment of ecological status of marine fish communities required by the marine strategy framework directive (MSFD) emphasises the need for fish-based ecological indices in marine waters. In this study we adapt five estuarine multimetric indices to the marine environment and apply them in three types of substrates, analysing the metrics responsible for the obtained patterns of ecological status. The results show inefficiency of the community degradation index (CDI) and the biological health index (BHI) in ecological status assessment and disagreement between the estuarine biotic integrity index (EBI), the estuarine fish community index (EFCI) and the transitional fish classification index (TFCI). Analysis of individual metrics suggests lack of representativeness and consideration for the particularities of each substrate's typical fish communities. None of the tested indices were efficient on the marine environment, urging the need for new marine indices that account for differences between types of substrate and depth.

Susceptibility of Didymopanax vinosum (Apiaceae) to insect herbivores was investigated in three sites of a cerrado mosaic--composed of campo cerrado (a grassland with scattered trees and shrubs), cerradão (a tall woodland) and cerrado sensu stricto (intermediate between the two)--situated in Cerrado Pé-de-Gigante, Santa Rita do Passa Quatro, SP, Brazil. We also examined the relationship of folivory with the composition and abundance of the insect herbivore fauna, and with several nutritional and defensive plant characteristics (water, nitrogen, cellulose, lignin, tannin leaf contents, and leaf toughness). We collected insects associated with D. vinosum every month, and we measured leaf damage every three months. In general, the annual folivory differed among sites. It reached the highest rates in site 1 and site 3: 7.33 and 8.5 percent, respectively. Only 1.32 percent of annual folivory was observed in site 2. These levels resulted from the higher abundance, in sites 1 and 3, of the thrips Liothrips didymopanacis (Phlaeothripidae), the most abundant herbivore sampled, responsible for more than 90 percent of the observed damage. However, no significant relationship was found between insect activity and the chemical and physical composition of the leaves. Our findings suggest that, at least in this species, other chemical compounds or variables related to plant apparency and resource availability to herbivores (e.g. plant architecture) might play a more decisive role in the spatial variation of folivory than the nutritional and defensive traits that were analyzed.

Down syndrome (DS) is the most common aneuploid disorder at birth. The life expectancy of persons with DS has improved over the last forty years and is now at about sixty years. Phenotypic characteristics include general hypotonia, maxillary hypoplasia with a small oral cavity and a somewhat larger appearing tongue, frequent constricted maxillary arch, nasal obstruction and others. This prospective study assesses the effects of rapid maxillary expansion (RME) on nasal patency of children with DS, using acoustic rhinometry (AR). Twenty four children with DS, aged 5 to 12 years, had been randomly allocated to the RME and control groups. AR was performed to these individuals prior to expansion, approximately one month after, post maximal expansion, and after a 5 months period of retention. The data between the two groups were compared. Rapid maxillary expansion produced a significant augmentation of nasal volume in children who had been treated (p < 0.05) compared to the control group; these results were stable through the period of retention.

A smoking prevention project in six European countries (European Smoking prevention Framework Approach) was developed, featuring activities for adolescents, schools and parents, including out-of-school activities. Consensus meetings resulted in agreement between the countries on goals, objectives and theoretical methods. Countries' specific objectives were also included. National diversities required country-specific methods to realize the goals and objectives. The community intervention trial was used as the research design. Since interventions took place at the community level, communities or regions were allocated at random to the experimental or control conditions. Complete randomization was achieved in four countries. At baseline, smoking prevalence among 23 125 adolescents at the start of the project was 5.6% for regular smoking and 4.0% for daily smoking. Smoking prevalence rates were higher among girls than boys in all countries as far as weekly smoking was concerned. Process evaluations revealed that the project's ambitions were high, but were limited by various constraints including time and delays in receiving funds. Future smoking prevention projects should aim to identify the effective components within the social influence approach as well as within broader approaches and on reaching sustained effects.