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The sulfonamide Schiff base compound (E)-4-((4-(dimethylamino)benzylidene)amino)-N-(5-methylisoxazol-3-yl)benzenesulfonamide was successfully prepared and fully characterized. The foremost objective of this study was to explore the molecular geometry of the aforementioned compound and determine its drug likeness characteristics, docking ability as an insulysin inhibitor, anticancer and antioxidant activities. The molecular structure of this compound was optimized using the B3LYP/6-311G+(d,p) level of theory. The compound was completely characterized utilizing both experimental and DFT approaches. Molecular electrostatic potential, frontier molecular orbitals, Fukui function, drug likeness, and in silico molecular docking analyses of this compound were performed. Wave functional properties such as localized orbital locator, electron localization function and non-covalent interactions were also simulated. The compound was screened for anticancer and antioxidant activities using in vitro technique. The observed FT-IR, UV-Vis, and 1 H NMR results compared with simulated data and both results were fairly consistent. The experimental and computational spectral findings confirm the formation of the Schiff base compound. Both π-π* and n-π* transitions were observed in both experimental and computational UV-Vis spectra. The examined compound followed to Pfizer, Golden Triangle, GSK, and Lipinski's rules. Consequently, it possesses a more favorable absorption, distribution, metabolism, excretion, and toxicity (ADMET) profile, making it a suitable candidate for non-toxic oral drug use. Moreover, the compound exhibited promising insulysin inhibition activity in an in silico molecular docking. The compound showed in vitro anticancer activity against A549 cancer cells with an IC 50 value of 40.89 μg/mL and moderate antioxidant activity., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

The sulfonamide Schiff base (C 16 H 14 N 4 O 3 S) was successfully synthesized and experimentally ascertained. The main purpose of this research is to investigate the geometry of the aforesaid molecule using both experimental and density functional theory (DFT) techniques and determine its drug likeness characteristics, docking ability as an insulysin inhibitor, and its NLO property. For the computational investigations the DFT approaches were utilized at the B3LYP level with the 6-311G+(d,p) basic set. The experimental results of the compound (such as FT-IR, UV-Vis, and 1 H NMR) were compared with simulated data. The both results were well and consistent with previously related published data. The obtained spectral results confirm the formation of the Schiff base compound. Both π-π* and n-π* interactions were found in experimental and computational UV-Vis spectra, as well as in the natural bond orbital (NBO) study. The molecular, electronic, covalent, and non-covalent interactions were analyzed using DFT studies. Both experimental and simulation results revealed that the compound is successfully formed and relatively stable. The compound with a lower band gap showed high chemical reactivity. The medicinal characteristics of the compound were evaluated using in silico medicinal methods. The investigated compound was also followed Pfizer, Golden Triangle, GSK as well as Lipinski's rules. Therefore, the compound has more favorable absorption, distribution, metabolism, excretion, and toxicity (ADMET) profile and it can be used as non-toxic oral drug candidate. The compound was exhibited good insulysin inhibitory activity and it has almost eighteen times higher non-linear optical properties than urea and three times higher than potassium dihydrogen phosphate (KDP)., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Here, we demonstrated the synthesis of a zinc based luminescent MOF, 1 (NDC = 2,6- naphthalenedicarboxylate) for the ratiometric detection of biomarker riboflavin (RBF; vitamin B 2 ) in water dispersed medium. Further, this MOF detected two other antibiotic drug molecules, nitrofurantoin (NFT) and nitrofurazone (NZF). The detection of these analytes is very quick (∼seconds), and the limit of detection (LOD) for RBF, NZF and NFT are calculated as 16.58 ppm, 47.63 ppb and 56.96 ppb, respectively. The detection of these analytes was also comprehended by solid, solution, cost-effective paper strip method i.e., triphasic identification capabilities. The sensor is reusable without losing its detection efficacy. The sensor further showed the recognition abilities of these antibiotics in real field samples (river water, urine and tablet) and RBF in vitamin B 2 pills and food samples (milk and cold drinks). The sensing merit of 1 urged us to fabricate of 1@cotton fabric composite, which exhibited the colorimetric detection of these analytes. In-depth experimental analysis suggested that the occurrence of photo-induced electron transfer (PET), fluorescence resonance energy transfer (FRET), and the inner filter effect (IFE) are the possible sensing mechanisms for the recognition of the antibiotics drug. The FRET mechanism is responsible for the recognition of RBF. The sensing mechanism is further supported by the theoretical analysis and the excited lifetime measurement., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

A hydrogen bonded ability metal organic framework (MOF, 1 Zn ) is used for the ultra-sensitive "turn-on" detection of hyperprolinemia biomarker with ultrafast (within 5 s) colorimetric response making the first MOF based hyperprolinemia biomarker sensor. The detection limit (4.46 ppb) is outperformed compared to all contemporary hyperprolinemia biomarker based sensors. Further, the sensor showed the recognition of biomarker in biological sample (human saliva). The detection of biomarker is also realized through colorimetric response (solution based and paper strip method). The mechanism of sensing is established through the electron transfer and the absorption caused emission (ACE). Moreover, the theoretical study is performed to support the sensing mechanism. The control titration of 1 Zn suggest that the free -NH 2 group of linker in 1 Zn is involved in supramolecular interaction (hydrogen bonding) with the carboxylic group present on biomarker results the facile occurrence of electron transfer and ACE. Consequently, the luminescence "turn-on" effect of 1 Zn for hyperprolinemia biomarker is observed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The exploration of smart sensors is of great significance for the selectivity, sensitivity, and ability to show the low detection limit for the target analyte. Here, we have used the linker H 2 L (5-((anthracen-9-ylmethyl)amino)isophthalic acid) for the construction of {[Cd(L)(DMF)(H 2 O) 2 ]·H 2 O} n ( 1 ) which is in order with the chromophore anthracene moiety and the free -NH functionality as a guest interaction site. This framework showed the luminescence recovery "turn-on" detection of the Al 3+ ion in an aqueous solution. An exhaustive mechanism study disclosed that the Lewis acid-base-type interaction between the Al 3+ ion and the -NH functionality of the linker in the framework revealed that the absorbance caused an enhancement for the "turn-on" sensing event. Besides the "turn-on" sensing event, the "turn-off" sensing phenomenon of 1 is also noticed when it detects the hazardous oxo-anions (MnO 4 - and CrO 4 2- ) with limit of detection values of 17.08 and 19.91 ppb, respectively. The detection of these diverse analytes are very fast (10 s) and they can also be recognized through a colorimetric response. The sensing mechanisms for these analytes are established by photoinduced electron transfer, Forster resonance energy transfer, and inert filter effect along with theoretical investigation. Furthermore, to show the sensing application of 1 in a versatile podium, a MOF gel composite, 1@AA (AA = Agar-Agar), was developed from 1 with AA. Interestingly, 1@AA showed the colorimetric detection of these analytes under UV light. Therefore, sensor 1 behaves as a smart sensory material for the recognition of the above analytes through a simultaneous "turn-on" and "turn-off" effect.

Herein, we describe the synthesis of a new fluorescent d 10 coordination polymer, [Zn 2 (CFDA) 2 (BPEP)] n ·nDMF (CP-1) under solvothermal reaction condition using zinc metal ion. In CP-1, Zn(II) ion along with CFDA and BPED ligand forms a 2-fold self-interpenetrated 3D coordination polymers. This CP-1 is characterized by the single crystal X-ray diffraction (SCXRD), powder X-ray diffraction (PXRD), infrared spectra, optical microscope image and thermogravimetric analysis and the framework is found to maintain its structural stability in different solvents. The framework (CP-1) detected antibiotics (NFT (nitrofurantoin) and NZF (nitrofurazone)) and organo-toxin trinitrophenol in aqueous dispersed medium. Apart from the fast responsive (10 s), the detection limit for them was found at ppb level. The detection of these organo-aromatics were also comprehended by the colorimetric response through solid, solution and low cost paper strip technique i.e., triple mode recognition capability. The probe is re-usable without changing in its sensing efficiency and in addition, it has been applied for the detection of these analytes in the real field specimens (soil, river water, human urine and commercial tablet). The sensing ability is established by in-depth experimental analysis and the life time measurement where mechanism such as photo induced electron transfer (PET), fluorescence resonance energy transfer (FRET), inner filter effect (IFE) was recognized. The presence of guest interaction sites on the linker backbone in CP-1 induces diverse supramolecular interaction with the targeted analytes results to bring them in proximity for the occurrence of these sensing mechanism. The Stern-Volmer quenching constant values of CP-1 for the targeted analytes are admirable and the low detection limit (LOD) values for NFT, NZF and TNP are found to be 34.54, 67.79 and 43.93 ppb respectively. Further, the DFT theory is carried out in details to justify the sensing mechanism., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Metal-organic frameworks (MOFs ) are excellent candidates for use in chemistry, material sciences and engineering thanks to their interesting qualitative features and potential applications. Quite interestingly, the luminescence of MOFs can be engineered by regulation of the ligand design, metal ion selection and encapsulation of guest molecules within the MOF cavity. Temperature is a very crucial physical parameter and the market share of temperature sensors is rapidly expanding with technology and medicinal advancement. Among the wide variety of available temperature sensors, recently MOFs have emerged as potential temperature sensors with the capacity to precisely measure the temperature. Lanthanide-based thermometry has advantages because of its ratiometric response ability, high quantum yield and photostability, and therefore lanthanide-based MOFs were initially focused on to construct MOF thermometers. As science and technology have gradually changed, it has been observed that with the inclusion of dye, quantum dots, etc . within the MOF cavity, it is possible to develop MOF-based thermometry. This review consolidates the recent advances of MOF-based ratiometric thermometers and their mechanism of energy transfer for determining the temperature (thermal sensitivity and temperature uncertainty). In addition, some fundamental points are also discussed, such as concepts for guiding the design of MOF ratiometric thermometers, thermometric performance and tuning the properties of MOF thermometers.

Since Galileo's time, the pendulum has evolved into one of the most exciting physical objects in mathematical modeling due to its vast range of applications for studying various oscillatory dynamics, including bifurcations and chaos, under various interests. This well-deserved focus aids in comprehending various oscillatory physical phenomena that can be reduced to the equations of the pendulum. The present article focuses on the rotational dynamics of the two-dimensional forced-damped pendulum under the influence of the ac and dc torque. Interestingly, we are able to detect a range of the pendulum's length for which the angular velocity exhibits a few intermittent extreme rotational events that deviate significantly from a certain well-defined threshold. The statistics of the return intervals between these extreme rotational events are supported by our data to be spread exponentially at a specific pendulum's length beyond which the external dc and ac torque are no longer sufficient for a full rotation around the pivot. The numerical results show a sudden increase in the size of the chaotic attractor due to interior crisis, which is the source of instability that is responsible for triggering large amplitude events in our system. We also notice the occurrence of phase slips with the appearance of extreme rotational events when the phase difference between the instantaneous phase of the system and the externally applied ac torque is observed., (© 2023 Author(s). Published under an exclusive license by AIP Publishing.)

Frequent use of antibiotics and the growth of industry lead to the pollution of several natural resources which is one of the major consequences for fatality to human health. Exploration of smart sensing materials is highly anticipated for ultrasensitive detection of those hazardous organics. The robust porous hydrogen bonded network encompassing a free-NH 2 moiety, Zn(II)-based metal-organic framework (MOF) ( 1 ), is used for the selective detection of antibiotics and toxic oxo-anions at the ppb level. The framework is able to detect the electronically dissimilar antibiotic sulfadiazine and nitrofurazone via fluorescence "turn-on" and "turn-off" processes, respectively. The antibiotic-triggered reversible fluoro-switching phenomena (fluorescence "on-off-on") are also observed by using the fluorimetric method. An extensive theoretical investigation was performed to establish the fluoro-switching response of 1 , triggered by a class of antibiotics and also the sensing of oxo-anions. This investigation reveals that the interchange of the HOMO-LUMO energy levels of fluorophore and analytes is responsible for such a fluoro-switchable sensing activity. Sensor 1 showed the versatile detection ability which is reflected by the detection of a carcinogenic nitro-group-containing drug "roxarsone". In view of the sustainable environment along with quick-responsive merit of 1 , an in situ MOF gel composite ( 1 @CS; CS = corn starch) is prepared using 1 and CS due to its useful potential features such as biocompatibility, toxicologically innocuous, good flexibility, and low commercial price. The MOF composite exhibited visual detection of the above analytes as well as antibiotic-triggered reversible fluoro-switchable colorimetric "on-off-on" response. Therefore, 1 @CS represents a promising smart sensing material for monitoring of the antibiotics and oxo-anions, particularly appropriate for the real-field analysis of carcinogenic drug molecule "roxarsone" in food specimens.

The highly luminescent complex [CdQ 2 (H 2 O) 2 ] (1) shows ultra-selectivity and high sensitivity to the explosive organo-toxin trinitrophenol (TNP). This detection is extremely fast with a high quenching constant (5.3 × 10 4 M -1 ) and a very low limit of detection (LOD) of 137 nM/59 ppb. This motivated us to detect the lethal carcinogenic arsenical drug roxarsone (ROX), which is reported here for the first time. The quenching constant and LOD for ROX using 1 were found to be 4.9 × 10 4 M -1 and 86 nM (or 37 ppb), respectively. Moreover, the probe also recognizes three lethal toxic oxo-anions (MnO 4 - , Cr 2 O 7 2- and CrO 4 2- ) with outstanding quenching constant (2.2 × 10 4 M -1 , 1.4 × 10 4 M -1 and 1.1 × 10 4 M -1 ) and very low LODs (141 nM/61 ppb, 178 nM/78 ppb and 219 nM/95 ppb). Compared to the previously reported homogeneous sensing nature of the discrete complexes, our complex showed the detection of toxic pollutants in a heterogeneous manner, which results in high recyclability and hence multi-cycle sensing capability. Interestingly, 1 shows the possibility for real-time monitoring through naked eye detection by visible colorimetric changes in solid, solution and strip paper methods, i.e. , triphasic detection ability. In addition, the sensor also exhibited the cross-sensing ability for these pollutants. The experimental sensing mechanism is strongly supported by the exhaustive theoretical investigation. Based on the fluorescence signal shown by each analyte, an integrated AND-OR logic gate is constructed. Furthermore, the sensing ability of 1 remains intact towards the detection of versatile real field samples including lethal carcinogenic arsenical drug roxarsone in the real food sample.

Four species of Aethina [Aethina (Aethina) vicina Grouvelle, 1894, Aethina (Idaethina) orientalis (Nietner, 1856), Aethina (Idaethina) subrugosa (Grouvelle, 1894b) and Aethina (Circopes) subquadrata (Motschulsky, 1858)] have been newly recorded from northeast Indian states. Hitherto, three species, viz., Aethina (Aethina) argus Grouvelle, 1890, Aethina (Aethina) cyaneipennis Grouvelle, 1903 and Aethina (Aethina) inconspicua Nakane, 1967 were known from Northeast India. The specific status of Aethina (Aethina) nigrocastanea Grouvelle, 1903 has been reestablished. Altogether eight species of Aethina are treated in this paper. The paper incorporates redescriptions of the genus, and species of Aethina from Northeast India with a key to these species.

Herein, an amine decorated Cd(II) metal-organic framework (MOF) with a uninodal 6-c topology was synthesized as a suitable platform for facile post-synthetic modification (PSM). The as-synthesized parent d 10 -MOF (1) with free -NH 2 centers, when functionalized with two different carbonyl substituents (1-naphthaldehyde and benzophenone) of varying conjugation, produces two novel luminescent MOFs (LMOFs) viz. PSM-1 and PSM-2. The judicious incorporation of carbonyl substituents into the skeleton of 1 was rationalized via ESI-MS, 1 H-NMR, FT-IR and PXRD analyses. Interestingly, both PSM-1 and PSM-2 show 'turn-on' luminescent behaviour in the presence of 1,4-dioxane with the limit of detection (LOD) as 1.079 ppm and 2.487 ppm, respectively, with prompt response time (∼55 s & ∼58 s, respectively). The inhibition of PET is comprehended to be the prime reason for luminescence enhancement upon interaction with the targeted analyte which was further validated from DFT calculations. In continuation, the PSM-MOFs were equally responsive towards 1,4-dioxane in several complex environmental matrices and cosmetic products. Additionally, vapor phase detection of 1,4-dioxane using PSM-MOFs has also been demonstrated as an additional advantage ensuring propagation of future research endeavour.

The single-crystal X-ray diffraction method was employed to characterize a rigid hydrated metal-organic framework (MOF), [Co 2 (MA)(INA)·2H 2 O] n , that displays an affinity toward water molecules under ambient conditions after dehydration. The fully dehydrated form was obtained using an environmental gas cell technique in a stepwise manner followed by its CO 2 -pressurized structure at 298 K using in situ crystallography.

This article aims to outline the characteristics of the blood flow conveying copper (Cu) and gold (Au) nanoparticles (NPs) through a non-uniform endoscopic annulus with wall slip under the action of electromagnetic force and Hall currents. The flow of blood with the suspension of hybrid nanoparticles in the annulus is induced by the peristaltic pumping. The governing equations are modeled and then simplified with the postulate of lubrication theory. The resulting non-dimensional momentum equation after simplification is solved analytically by employing the He's homotopy perturbation method (HPM) with the computational software Mathematica program (version 11). The influential role of emerging physical parameters on the physiological features related to the blood flow is inferred graphically and physically. The analytical outcomes reveal that Hall parameter has a diminishing behavior on the blood flow while the inverse impact is endured for mounting Hartmann number. Electromagnetic field and Hall currents offer a superlative mode for regulating blood flow at the time of surgery. An increment in the volume fraction of nanoparticles causes a drop in the blood temperature profile. The trapping phenomenon is also explored with the help of contours. An expansion in Hartmann number reduces the size of entrapped bolus and ultimately vanishes when Hartmann number is very large. This prospective model may be applicable in electromagnetic micro-pumps, medical simulation devices, heart-lung machine (HLM), drug carrying and drug transport systems, cancer diagnosis, tumor selective photothermal therapy, etc., (Copyright © 2021 Elsevier Inc. All rights reserved.)

A new Schiff base, ( E )-benzyl-2-(4-hydroxy-2-methoxybenzylidene)hydrazinecarbodithioate (compound 1 ) has been synthesized and experimentally characterized by the IR, UV-Vis, 1 H-NMR and mass spectroscopies. The theoretical study of the synthesized compound was evaluated using the density functional theory (DFT) at B3LYP/6-31G+(d,p) basis set. The electronic absorption spectrum of compound 1 was evaluated using time-dependent density functional theory. Besides, in silico studies were done for the prediction of absorption, distribution, metabolism and excretion profiles of compound 1 . According to the result, the theoretical data were well fitted with the experimental values. The studied compound has low chemical reactivity and high kinetic stability. In the molecular electrostatic potential map, the negative and positive potential sites were found around electronegative atoms and hydrogen atoms of compound 1 , respectively. The 97.75% Lewis and 2.25% non-Lewis structure were present in the studied molecule. The molecular docking results reveal that compound 1 can be used as antituberculosis agent as compare to ethambutol., Competing Interests: The authors declare no conflict of interest., (© 2021 The Authors. Published by Elsevier Ltd.)

The rheological perspective of blood flow with the suspension of metallic or non-metallic nanoparticles through arteries having cardiovascular diseases is getting more attention due to momentous applications in obstructed hemodynamics, nano-hemodynamics, nano-pharmacology, blood purification system, treatment of hemodynamic ailments, etc. Motivated by the novel significance and research in this direction, a mathematical hemodynamics model is developed to mimic the hemodynamic features of blood flow under the concentration of hybrid nanoparticles through an inclined artery with mild stenosis in the existence of dominating electromagnetic field force, Hall currents, heat source, and porous substance. Copper (Cu) and copper oxide (CuO) nanoparticles are submerged into the blood to form hybrid nano-blood suspension (Cu-CuO/blood). The attribute of the medium porosity on the blood flow is featured by Darcy's law. The mathematical equations describing the flow are formulated and simplified under mild stenosis and small Reynolds number assumptions. To determine the analytical solution of the resulting nonlinear momentum equation, the homotopy perturbation method (HPM) is employed. Several figures are graphed to assess the hemodynamical contributions of various intricate physical parameters on blood flow phenomena through the inclined stenosed artery. Significant outcomes from graphical elucidation envisage that the hemodynamic resistance to the blood flow is reduced due to the dispersion of more hybrid nanoparticles in the blood. The hemodynamic resistance (impedance) increases approximately two times by dispersing 0.11% hybrid nanoparticles in the blood flow. The temperature of Cu-CuO/blood is found to be lower in comparison to Cu-blood and pure blood. Intensification of Hall parameter attenuates the wall shear stress at the arterial wall. The trapping phenomena are also outlined via streamline plots which exemplify the blood flow pattern in the stenosed artery under the variation of the emerging parameters. As anticipated, the addition of a large number of hybrid nanoparticles significantly modulates the blood flow pattern in the stenotic region. The novel feature of this model is the impressive impact of Hall currents on hybrid nanoparticle doped blood flow through the stenosed artery. There is another piece of significance is that HPM is the most suitable method to handle the nonlinear momentum equation under the aforementioned flow constraints. Outcomes of this simulation may be valuable for advanced study and research in biomedical engineering, bio-nanofluid mechanics, nano-pharmacodynamics., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Two new dicyanamide bridged multinuclear Zn complexes, [Zn 2 (L 1 )(µ 1,5 -dca) 2 (µ 1 -dca)] n (1) and [Zn 2 (L 2 )(µ 1,5 -dca) 2 (µ 1 -dca)] n (2) have been synthesized using N 2 O 4 -based pro-ligands (H 2 L 1  = N,N'-bis(5-bromo-3-methoxysalicylidenimino)-1,3-diaminopropane, H 2 L 2  = N,N'-bis(3-ethoxysalicylidene)-2,2-dimethyl-1,3-propanediamine) and characterized by microanalytical and spectroscopic techniques. Both complexes are stable in solution and solid-state. Thermogravimetric analysis (TGA) findings showed that complexes are stable at room temperature. Single-crystal X-ray diffraction (SCXRD) has proven that complexes are identical structures where two zinc metal ions are crystallographically independent. The directional properties of dicyanamide co-ligands via µ 1,5 bridging have resulted in different connectivity of zinc metal ions leading to 1D templates. SCXRD revealed some notable non-covalent interactions (π⋯π, C-H····π, and H-bonding) in their solid-state crystal structures. 1-2 have strong fluorescence behaviour over pro-ligands, which may be quenched in the presence of various electron-deficient explosive nitroaromatic compounds (epNACs). Complex 2 fluorescence intensity is sharper than 1; hence the former retained high sensitivity and selectivity for trinitrophenol (TNP). The enhancement of fluorescence mechanism, detection limit (LOD), and the quenching constant (K SV ) have been calculated using the Stern-Volmer equation (SV), where the K SV value for TNP is found to be 1.542 × 10 4 M -1 . The solution phase quenching mechanism has been rationalized by (a) electrostatic interactions through charge-transfer complex, (b) photo-induced electron transfer (PET) by the HOMO-LUMO energy gap via DFT, and (c) fluorescence resonance energy transfer (FRET). Finally, complex 2 is applied as a sensor by turn-off fluorescence response to detecting TNP nitroaromatics in the DMF medium., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Herein, two luminescent porous networks ( CMERI-1 & CMERI-2 ) have been reported for the efficient detection of formaldehyde (FA) from aqueous medium. Judicious solvent screening using a high-throughput solvothermal procedure leads to two completely different metal-organic framework (MOFs) with different architectures. It is perceived that the framework CMERI-1 shows better sensitivity with a very short response time (1 min) in the realm of FA detection due to the facile imine (-N═CH-) formation, which is restricted in the case of CMERI-2 . The fluorescence "turn-on" behavior is ascribed due to the inhibition of photoinduced electron transfer (PET) (from amine subunit to secondary building unit) process. The detection limits of CMERI-1 & CMERI-2 toward FA in aqueous medium were found to be 0.62 μM (0.019 ppm) and 1.39 μM (0.041 ppm), respectively, that lie far below the intracellular concentration of formaldehyde (100-400 μM). In addition, MOF-based hydrogel membrane was fabricated, which shows vapor-phase detection of FA, which is hitherto unexplored in this realm. Moreover, the response mechanisms of MOFs are supported by density functional theory (DFT) and Fukui indices analysis. The high stability of the porous frameworks along with its interesting sensing features such as fast recognition phenomenon, appreciable detection limit, etc. instigated us to explore its real-world applicability in various food sample and water analyses. In view of the modular design principle of our polymeric probe, the proposed approach could open a new horizon to construct powerful sensing materials for the ultrafast detection of other industrial pollutants in the domain of supramolecular and analytical chemistry.

Sap beetle, Epuraea ocularis Fairmaire usually lays eggs and breeds on fermenting overripe fruits, and larvae pass through different instars before pupating on soil. In laboratory condition, mating pairs of adults copulated and females laid eggs in clusters; larva hatched out in 1 to 2 days, passed through four instars; mature larva migrated to soil for pupation. Larval development took about 12 to 17 days; and adult hatched out of pupa in about 4 to 5 days. Detailed morphology of egg, larva and pupa is presented herein, and significance of larva in taxonomy of beetles has been indicated.

The design and synthesis of efficient sensor materials with fast-responsive and ultrasensitive detection ability is critical to monitor ecological safety, supervise human health, control industrial wastes, and govern food quality among others. Metal-organic frameworks (MOFs) or coordination polymers (CPs) are a new class of porous crystalline materials that have emerged in several potential applications in last two decades. In particular, applications of MOFs as sensory scaffolds for the detection of hazardous pollutants have attracted researchers due to their fabulous structural characteristics and wide range of pore environment tunability. Among several transducer procedures, the luminescence detection of a particular analyte is immensely desirable as it is easy to handle and cost effective, where visual changes in physicochemical attributes can be comprehended via a quick naked eye detection. The porous nature of MOFs facilitates the pre-concentration of target analytes within the pore structure and provides superior host-guest interaction with good detection limits when compared to conventional materials. To this end, guest-induced fluorescence switching in sensory MOFs with good recyclability and unique detectable fingerprints are of particular importance to benefit futuristic monitoring aptitudes and promises environmental remediation. In this review, we present the latest literature based on the analyte-responsive modulation of fluorescence intensity in MOFs towards the detection of target pollutants and discuss the underlying sensing mechanism, which can assist in developing new useful nano-scale devices and sensors.

In the present investigation, the supercritical carbon dioxide (SC-CO2) extracts of small cardamom (SC) and yellow mustard (YM) seeds have been investigated for their efficacies in combating type 2 diabetes in streptozotocin-induced Wistar albino rats. Fasting blood glucose (FBG) levels in the rats were monitored on days 8, 15 and 21. On day 15, FBG level reduced appreciably by 31·49 % in rats treated with SC seed extract and by 32·28 % in rats treated with YM seed extract, comparable to metformin (30·70 %) and BGR-34 (a commercial polyherbal drug) (31·81 %) administered rats. Either extract exhibited desirable effects on hepatic glucose-6-phosphatase, glucose-6-phosphate dehydrogenase (G6PD) and catalase activities in controlling diabetes. A molecular docking exercise was conducted to identify specific compounds in the extracts which possessed augmenting effect on G6PD. The results revealed that all the bioactive compounds in the extracts have binding affinities with the enzyme and contributed to the antidiabetic efficacies of the extracts as G6PD augmenters. The effects of the extracts on insulin sensitivity and glucose uptake were investigated using non-invasive modelling by iHOMA2 software. This in vitro approach indicated that extract administration resulted in increased both insulin sensitivity of the liver and glucose uptake in the gut. The findings of the present study attest these SC-CO2 extracts of the spices as safe alternatives of metformin and BGR-34 in combating type 2 diabetes and could be safely subjected to clinical studies. These extracts could also be employed in designing proactive food supplements in mitigating the metabolic disorder.

Benzyl-3-N-(2,4,5-trimethoxyphenylmethylene)hydrazinecarbodithioate (compound 1) is a bidentate and nitrogen-sulfur containing Schiff base, which has been synthesized by the condensation reaction of S-benzylndithiocarbazate and 2,4,5-trimethoxybenzaldehyde. The theoretical calculations of the mentioned compound have been carried out using the more popular density functional theory method, Becke-3-Parameter-Lee-Yang-Parr (B3LYP) in 6-31G+(d,p) basis set. The computational results of the compound were compared with the obtained experimental value. Moreover, the highest occupied molecular orbital, the lowest unoccupied molecular orbital, molecular electrostatic potential, chemical reactivity parameters and natural bond orbital of the optimized structure have been evaluated at the same level of theory. Furthermore, the UV-Vis spectrum of the compound has been carried out for the better understanding of electronic absorption spectra with the help of the time-dependent density functional theory at room temperature. Besides, the molecular docking simulation of the mentioned molecule with target protein was also investigated. In addition, in silico studies were performed to predict absorption, distribution, metabolism, excretion and toxicity profiles of the designed compound. The results indicated that the theoretical data have well correlated with the observed values. The narrow frontier orbital gap indicated that the eventual charge transfer interaction occurs within the studied molecule and showed high chemical reactivity. The global reactivity values showed that the compound is soft molecule, electrophilic species and has strong binding ability with biomolecules. The molecular electrostatic potential structure indicated that the negative and positive potential sites are around electronegative atoms and hydrogen atoms of studied compound, respectively. The natural bond orbital data revealed that the compound contains 97.42% Lewis and 2.58% non-Lewis structure. The intra and inter-molecular charge transfers process occur within the studied compound. The studied compound showed more binding energy (-6.0 kcal/mol) with target protein than hydroxychloroquine (-5.6 kcal/mol). The absorption, distribution, metabolism, excretion and toxicity investigation predicted that the compound has good drug like character., Competing Interests: The authors have no conflicts of interest to declare., (© 2020 Elsevier B.V. All rights reserved.)

Background: Controlling cariogenic biofilm formation by plant extracts could add to preventive strategies to dental caries., Objective: To evaluate in vitro the role of Aerva Sanguinolenta ethanolic extract on biofilm-induced microbial human enamel demineralization., Methodology: The prepared enamel sections of study group (SG), positive control group (PCG), and negative control group (NCG) were immersed in 2 ml of 0.2% ethanolic extract of A. sanguinolenta, 0.12% chlorhexidine, and distilled water, respectively, for 2 min before subjecting to closed batch culture technique utilizing mono- or dual-species culture media of Streptococcus mutans and Lactobacillus acidophilus. Quantification of biofilm and demineralization of enamel was performed by crystal violet (CV) assay and scanning electron microscope (SEM) attached to energy-dispersive X-ray analysis, respectively., Statistical Analysis: Two-way ANOVA and Tukey's test were used for analysis., Results: CV assay of biofilm recorded the highest and lowest optical absorbance value in NC3 (2.728660) and PC3 (0.364200), respectively. Thus, biofilm formation is highest in NCG and lowest among PCG. Surface roughness and porosity in enamel are greatest among NCG and lowest among SG as evident by SEM. Wt% of calcium (S3 47.7170) and phosphorus ion (S3 22.7330) was highest in SG, closely resembling that of B enamel (Ca = 41.9530, P = 19.6650). Wt% of oxygen is lowest in SG (S3 28.8920) and resembles baseline O 2 (37.4950). Thus, the amount of biofilm formation is moderate and amount of demineralization of enamel is least among SGs., Conclusion: Enamel exposed to 2 ml of 0.2% solution of A. sanguinolenta for 2 min could fairly inhibit formation of biofilm and positively inhibit underlying demineralization in cariogenic environment., Competing Interests: None

In spite of having a remarkable anti tumor activity against a wide variety of cancers, the clinical effectiveness of the major chemotherapeutic drug paclitaxel is often limited by the issues of drug resistance that hampers the therapeutic effectiveness of the drug. The combination of proton pump inhibitor with paclitaxel is an effective approach to overcome therapeutic resistance caused by the acidic microenvironment (Warburg effect) in tumor. In the present study a new simple, precise and selective liquid chromatography tandem mass spectrometry method was developed for quantification of paclitaxel and lansoprazole using esomeprazole as an internal standard and applied for the pharmacokinetic study of investigational paclitaxel - lansoprazole loaded PLGA nanoparticles. The developed method quantifies both the drugs simultaneously irrespective of their dissimilar stability concerns. The detection was exercised with multiple reaction-monitoring mode in positive ionization that yielded highly intense response of parent-product (m/z) transition pair 854.4 → 286.1, 370.1 → 251.9 and 346 → 198 for paclitaxel, lansoprazole and Esomeprazole respectively. The chromatographic separation was achieved using phenomenex Kinetex 5 μ C18 100A 50 × 3.0 mm column and a gradient mobile phase combination of ammonium acetate in deionized water (pH 6.8, 2 mM, w/v) and acetonitrile spiked with formic acid (1:1000, v/v ). This method showed good linearity over a concentration range of 10-320 ng/mL and 100-3200 ng/mL with correlation coefficient (R 2 ) 0.98 and 0.94 for paclitaxel and lansoprazole respectively. Using liquid liquid extraction process both the drugs were extracted from rat plasma. The intra- and inter-day precision and accuracy values were within the variability limits and both the analytes were found to be stable throughout the freeze-thaw, auto-sampler, bench top and long term stability studies. The liquid chromatography tandem mass spectrometry method was successfully validated in accordance with United States Food and Drug administration guidelines and the results were within the acceptable limits. The liquid chromatography tandem mass spectrometry method was successfully utilized for the pharmacokinetic investigation of experimental paclitaxel - lansoprazole loaded PLGA nanoparticles in rat plasma., (Copyright © 2019 Elsevier B.V. All rights reserved.)

In advanced medication, drug-loaded polymeric nanoparticles (NPs) appeared as a novel drug delivery system with lots of advantages over conventional medicines. Despite all the advantages, NPs do not gain popularity for manufacturing hurdles. The study focused on the formulation difficulties and implementation of statistical design to establish an effective model for manufacturing NPs. In this study, physico-chemical properties of the drug and polymer (PLGA) were incorporated to understand the mechanistic insights of nanoformulations. Primarily, the process controlling parameters were screened by Plackett-Burman design and the critical process parameters (Cpp) were further fabricated by Box-Behnken design (BBD). The TLM-PLGA-NPs (telmisartan loaded PLGA NPs) exhibited particle size, encapsulation efficiency and zeta potential of 232.4 nm, 79.21% and -9.92 mV respectively. The NPs represented drug loading of 76.31%. Korsmeyer-Peppas model ( R 2 = 0.925) appeared to be the best fitted model for in vitro release kinetics of NPs. The model identified Fickian diffusion of TLM from the polymeric nanoparticles. The ANOVA results of variables indicate that BBD is a suitable model for the development of polymeric NPs. The study successfully identified and evaluated the correlation of significant parameters that were directly or indirectly influencing the formulations which deliberately produce desired nanoparticles with the help of statistical design., Competing Interests: We have no competing interests.

Among the secondary metabolites which are widely distributed in plants and foods in plant origin flavonoids is important one. Flavonoids have antioxidant activities as free radical scavenging action. They also have anti-inflammatory, antiulcer and anti-carcinogenic activities. Diosmin and hesperidin, the metabolites of which are diosmetin and hesperitin respectively are considered in the present study. Diosmetin has anticancer, antioxidant and blood lipid lowering activities. It also enhances venous tone and microcirculation and by reducing systemic oxidative stress it protects capillaries. Hesperitin also has antioxidant, anti-inflammatory, blood lipid and cholesterol lowering, anti-carcinogenic activities. In the present study efforts were given to develop and validate a bioanalytical method for simultaneous estimation of diosmetin and hesperitin in human plasma by liquid chromatography electron spray ionization mass spectrometry with an application to the analysis of plasma samples obtained from the comparative pharmacokinetic studies on healthy human volunteers under the framework of bioequivalence study. The developed method for simultaneous determination and quantification of diosmetin and hesperitin in human plasma was validated as per the US-FDA guidelines. The validation parameters found within the specified regulatory limit, hence acceptable. The present method also has a short run time (6.0 min) and easy extraction process. The developed method was found to be simple, specific, highly selective, sensitive and reproducible. This was applied for the analysis of the volunteer plasma samples. On the basis of comparison of the AUC0-t, the relative bioavailability of the test preparation was found 100.94 and 95.09% for diosmetin and hesperitin respectively of that of the reference preparation., (© The Author(s) 2019. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)

Porous solids that can be switched between different forms with distinct physical properties are appealing candidates for separation, catalysis, and host-guest chemistry. In this regard, porous organic cages (POCs) are of profound interest because of their solution-state accessibility. However, the application of POCs is limited by poor chemical stability. Synthesis of an exceptionally stable imine-linked (4+6) porous organic cage (TpOMe-CDA) is reported using 2,4,6-trimethoxy-1,3,5-triformyl benzene (TpOMe) as a precursor aldehyde. Introduction of the -OMe functional group to the aldehyde creates significant steric and hydrophobic characteristics in the environment around the imine bonds that protects the cage molecules from hydrolysis in the presence of acids or bases. The electronic effect of the -OMe group also plays an important role in enhancing the stability of the reported POCs. As a consequence, TpOMe-CDA reveals exceptional chemical stability in neutral, acidic and basic conditions, even in 12 m NaOH. Interestingly, TpOMe-CDA exists in three different porous and non-porous polymorphic forms (α, β, and γ) with respect to differences in crystallographic packing and the orientation of the flexible methoxy groups. All of the polymorphs retain their crystallinity even after treatment with acids and bases. All the polymorphs of TpOMe-CDA differ significantly in their properties as well as morphology and could be reversibly switched in the presence of an external stimulus., (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Paclitaxel (PTX) is a major chemotherapeutic drug that is effective against a wide variety of cancers, particularly breast, ovarian and lung cancer. For a weakly basic chemotherapeutic drug such as PTX, the development of the acidic tumor microenvironment (Warburg effect) has a remarkable impact on therapeutic resistance. The present approach takes advantage of the acidic tumor microenvironment by incorporating lansoprazole (LAN), a proton pump inhibitor (PPI), with PTX as a potent therapeutic combination that is capable of reversing PTX resistance. To deliver optimal amounts of the drugs to neoplastic cells, a nano drug delivery system was selected. To design the nanoformulation process in a limited framework, typical formulation parameters were optimized and validated by the application of response surface methodology (RSM) using Box-Behnken design (BBD). On the basis of critical quality aspects, the experimental design helped to determine the optimal particle size (243.7 nm), zeta potential (-9.14 mV) and encapsulation efficiencies (88.91% and 80.35% for PTX and LAN respectively). The optimized formulation (PTX-LAN-PLGA-NPs) exhibited sustained in vitro release profiles over 384 hours for both the encapsulated drugs. The Korsmeyer-Peppas model was found to be the best fitted model for the release kinetics, where the release mechanism follows Fickian diffusion. In in vitro anti-tumor efficacy experiments using Michigan Cancer Foundation-7 (MCF-7) breast cancer cells, the PTX-LAN-PLGA-NPs exhibited a steep decrease in cell viability compared to the pure drugs. Taken together, the results strongly support that incorporation of PTX and LAN in nanoparticles (NPs) is a promising approach for cancer chemotherapy., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Background & Objectives: In our previous investigation, oral administration of 1,8- cineole-rich supercritical carbon dioxide extract of small cardamom seeds in Wistar albino rats resulted in achieving normal fasting blood glucose (FBG) and serum cholesterol levels. The objective of this study was to further protect the aforesaid extract and to enhance its in vivo therapeutic efficacies in redressing type 2 diabetes and hypercholesterolemia, by encapsulating it as nanoliposomes. Patents related to nanoliposomes have been revised thoroughly., Methods: PEGylated nanoliposomes of the aforesaid extract were formulated using soya phosphatidylcholine and Tween 80 by probe-sonication. These nanoliposomes were subjected to in vitro characterizations and were orally administered to Wistar albino rats at three different doses viz. 550, 175 and 55 mg/kg b.w. for detailed investigation of their antidiabetic and hypocholesterolemic efficacies., Results: FT-IR, DSC and XRD analyses, HLB value (16), entrapment efficiency (84%) and release kinetics (obeying Higuchi model) revealed that the nanoliposomes were o/w type and were hydrophilic. They exhibited appreciable in vitro antioxidant potency (59% DPPH scavenging activity) owing to a synergistic consortium of antioxidants present therein. Oral administration of the liposomes in rats at 550 mg/kg b.w. could restore their normal FBG levels and serum lipid profiles on day 35, with desirable up-down regulations of related key enzymes. The iHOMA2 model could successfully predict the effects of nanoliposomes on insulin sensitivity and glucose uptake in rat liver and brain, respectively., Conclusion: Nanoliposome of 1,8-cineole rich extract of small cardamom seeds is a new biotherapeutic in redressing type 2 diabetes and hypercholesterolemia., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

The prevalence of hypertension is very common amongst the diabetic patients and is reported as the major cause of mortality in diabetes. Pioglitazone reported to have an ability to alter the blood cholesterol level and cardioprotective efficiency along with its antidiabetic activity. Telmisartan, through activation of PPAR-γ receptor exerts insulin sensitizing property in addition to its primary cardioprotective efficiency. Theoretically, a combination of pioglitazone and telmisartan may be beneficial to effectively control the high blood glucose level and management of coexisting cardiovascular complication in diabetes. The aim of this research was to experimentally evaluate the pharmacokinetic interaction of pioglitazone and telmisartan when are coadministered in rat. Pioglitazone and telmisartan were administered orally as a single dose individually and in combination to the rats. The plasma samples of the pharmacokinetic study were analyzed using a validated LCMS method. The acute toxicity of the combination with a high dose in rats was also evaluated as a part of the determination of its safety profile. There was no significant change in pharmacokinetic parameters were resulted due to the coadministration of pioglitazone and telmisartan in rat. Absence of major toxicological effect supports the in vivosafety of the combination., (Copyright © 2017 Elsevier Inc. All rights reserved.)

Oral paclitaxel (PTXL) formulations freed from cremophor ® EL (CrEL) is always in utmost demand by the cancerous patients due to toxicities associated with the currently marketed formulation. In our previous investigation [Int. J. Pharm. 2014; 460:131], we have developed an oral oil based nanocarrier for the lipophilic drug, PTXL to target bioavailability issue and patient compliance. Here, we report in vivo antitumor activity and 28-day sub-chronic toxicity of the developed PTXL nanoemulsion. It was observed that the apoptotic potential of oral PTXL nanoemulsion significantly inhibited the growth of solid tumor (59.2 ± 7.17%; p < 0.001) without causing any explicit toxicity. The 6.5 mg/kg and 3 mg/kg oral PTXL nanoemulsion dose did not cause any notable alteration in haematological, biochemical/structural characteristics during 28-day sub-chronic toxicity studies in the experimental mice. Whereas, the toxicity of 12.8 mg/kg body weight dose showed decrease in RBC, haemoglobin and neutrophil counts. In contrast, marketed PTXL (Taxol ® ) was found to be comparatively more toxic to the experimental animals. Taxol ® treatment resulted glomerulonephritis in histopathological examination, which could be correlated with increased level of creatinine and associated nephrotoxicity. This investigations conclude that the developed oral nanoemulsion presents a viable therapeutics bio-system to step towards clinical application as well as substitute CrEL based PTXL formulations., (Copyright © 2017 Elsevier Inc. All rights reserved.)

Management of cardiovascular risk factors in diabetes demands special attention due to their co-existence. Pioglitazone (PIO) and telmisartan (TLM) combination can be beneficial in effective control of cardiovascular complication in diabetes. In this research, we developed and validated a high throughput LC-MS/MS method for simultaneous quantitation of PIO and TLM in rat plasma. This developed method is more sensitive and can quantitate the analytes in relatively shorter period of time compared to the previously reported methods for their individual quantification. Moreover, till date, there is no bioanalytical method available to simultaneously quantitate PIO and TLM in a single run. The method was validated according to the USFDA guidelines for bioanalytical method validation. A linear response of the analytes was observed over the range of 0.005-10 µg/mL with satisfactory precision and accuracy. Accuracy at four quality control levels was within 94.27%-106.10%. The intra- and inter-day precision ranged from 2.32%-10.14 and 5.02%-8.12%, respectively. The method was reproducible and sensitive enough to quantitate PIO and TLM in rat plasma samples of a preclinical pharmacokinetic study. Due to the potential of PIO-TLM combination to be therapeutically explored, this method is expected to have significant usefulness in future.

The linker 5-(benzylamino)isophthalic acid (H2L) forms a robust 2D Zn(ii)-coordination polymer, {[Zn 8 (L) 6 (μ 3 -OH) 4 (H 2 O) 6 ]·(DMF)·(H 2 O) 2.5 } n (1Zn) consisting of [Zn 4 (μ 3 -OH) 2 ] 6+ SBUs. It further shows strong ππ interactions between the layers to form an overall 3D structure with well-defined channels. Metal bound water molecules are directed towards these channels. Upon heating, these metal bound water molecules are lost along with lattice solvent molecules to afford coordinatively unsaturated metal centers that can be utilized as an efficient heterogeneous catalyst for the C-C bond forming nitroaldol or Henry reaction. The solid catalyst can be recycled at least three times without losing its catalytic activity. The easily accessible channels in 1Zn are found suitable for the SC-SC transmetalation reaction with Cu(ii) producing 1Cu. The catalytic activity of 1Cu is found to be comparable to that of 1Zn. Furthermore, the framework 1Zn gives high emission in DMSO, which can be efficiently quenched by trace amounts of nitro explosives. All the complexes have been characterized by single crystal X-ray diffraction, powder X-ray diffraction, IR, thermogravimetry and elemental analysis.

Background: Enalapril (EPL) is an angiotensin-converting enzyme inhibitor for the treatment of hypertension and chronic heart failure. Enalaprilat (EPLT) is an active metabolite that contributes to the overall activity of EPL., Aim: To quantitate EPL along with its metabolite EPLT using LC-MS/MS, a bioanalytical method was developed and validated with tolbutamide in human plasma using a protein precipitation technique., Results: The sensitive and selective method has an LLOQ of 1 ng/ml with a linearity range of 1-500 ng/ml for both EPL and EPLT using 300 µl of plasma without any matrix effect., Conclusion: Linearity, specificity, accuracy, precision and stability, as well as its application to the analysis of plasma samples after oral administration of 20 mg of EPL maleate in healthy volunteers demonstrate applicability to bioavailability/bioequivalence studies., Competing Interests: Financial & competing interests disclosure The authors also acknowledges Pharmland JV Leninskaja STR, Nesviz Belarus for providing the necessary gift samples of enalapril maleate. The authors, D Halder and S Dan, are grateful to University Grants Commission (UGC), New Delhi, India for providing the fellowship. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

A sensitive, specific and reproducible liquid chromatography coupled to tandem mass spectrometric method was developed and validated for the estimation of ciprofloxacin, an extensively used second-generation quinolone antibiotics, in human plasma. A liquid-liquid extraction of ciprofloxacin and the internal standard, ofloxacin, has been approached from the biological matrix using chloroform. Chromatographic separation was achieved in positive ion modes, isocratically on a 3.5 μm C18 analytical column (75 mm×4.6 mm, i.d.) with 0.2% formic acid solution in water: methanol (10:90, v/v) as mobile phase, at a flow rate of 0.5 mL.min -1 . The MS/MS ion transitions were monitored as 332.0→231.3 for ciprofloxacin and 362.2→261.0 for IS. The method showed good linearity in the range of 0.01-5.00 μg.mL -1 ( r 2 >0.99) with a good precision (3.37-12.60%) and accuracy (87.25-114%). At the same time, ciprofloxacin was found to be stable during stability studies viz. bench-top, auto-sampler, freeze-thaw cycle and long-term. The developed and validated method was successfully applied to measure plasma ciprofloxacin concentrations in a single dose bioequivalence study., (© Georg Thieme Verlag KG Stuttgart · New York.)

Poor aqueous solubility and unfavourable de-esterification of olmesartan medoxomil (a selective angiotensin II receptor blocker), results in low oral bioavailability of less than 26%. Improvement of oral bioavailability with prolonged pharmacodynamics activity of olmesartan in Wistar rats had been approached by nanoemulsification strategy in our previous article [Colloid Surface B, 115, 2014: 286]. In continuation to that work, we herewith report the biodistribution behaviour and 28-day repeated dose sub-chronic toxicity of olmesartan medoxomil nanoemulsion in Wistar rats following oral administration. The levels of olmesartan in collected biological samples were estimated using our validated LC-MS/MS technique. Our biodistribution study showed significantly higher brain concentrations of olmesartan (0.290 ± 0.089 μg/mL, 0.333 ± 0.071 μg/mL and 0.217 ± 0.062 μg/mL at 0.5, 2.0 and 8.0 h post dosing, respectively) when administered orally as nanoemulsion formulation as compared to the aqueous suspension. In addition, the olmesartan nanoemulsion was found to be safe and non-toxic, as it neither produced any lethality nor remarkable haematological, biochemical and structural adverse effects as observed during the 28-days sub-chronic toxicity studies in experimental Wistar rats. It is herewith envisaged that the developed nanoemulsion formulation approach for the delivery of olmesartan medoxomil via oral route can further be explored in memory dysfunction and brain ischemia, for better brain penetration and improved clinical application in stroke patients., (Copyright © 2016 Elsevier Inc. All rights reserved.)

Background: Groundnut seeds are an important source of bioactive phenolic compounds with noteworthy antioxidant capacity, which may be enhanced by the microwave roasting process. The aim of this work is   to study the changes in antioxidant activity in groundnut seeds during microwave roasting, as a function of roasting time and extract concentration, in order to maximise the phenolic content and antioxidant activity of roasted seeds., Methods: The study was conducted to evaluate total phenolic content (TPC), total flavonoid content (TFC), and antioxidative activity of methanolic (GME), ethanolic (GEE), and chloroform (GCE) extracts and methanolic extract of oil (GMO) from groundnut seeds exposed to microwaves. The antioxidant activity was investigated using several assays, namely phosphomolybdenum assay, DPPH radical scavenging activity, H2O2 scavenging activity, hydroxyl radical scavenging activity and reducing power., Results: The microwave roasting process significantly increased the TPC, whilst the TFC decreased with roasting time. Antioxidant activity increased with increased roasting time and extract concentration in all extracts. Antioxidant activity increased significantly at lower concentrations; however, the rate of increment decreased gradually as the concentration of the solvent extract increased. Thus, among all the extracts, methanol extracts at all roasting times and extract concentrations appeared to display the highest effectiveness. The various scavenging activities of the samples are ranked in the following order: GME > GEE > GCE > GMO, in both raw and roasted samples., Conclusions: Both roasting time and extract concentration were found to be critical factors in determining the overall quality of the product. This investigation is important to determine optimum roasting conditions, in order to maximise the anti-oxidative health benefits of the Bangladeshi groundnut cultivar.

A partially fluorinated, angular tetracarboxylic acid linker (H4L) incorporating a pendant amine moiety forms a three-dimensional Zn(II) framework, 1. The structure consists of paddle-wheel Zn2(CO2)4 secondary building units (SBUs) and Zn12(CO2)24 supramolecular building blocks (SBBs). Thermal stability of 1 is found to be low. However, it undergoes transmetalation reaction with Cu(II) at room temperature without losing crystallinity affording an isostructural framework, 1Cu. Framework 1Cu is thermally robust and allows generation of the solvent-free porous framework 1Cu' upon activation with coordinatively unsaturated metal centers. Framework 1Cu' exhibits water stability and at 77 K, adsorbs 2.56 wt % of H2 up to 1 bar that significantly increases to 4.01 wt % at 13 bar. Also, this framework gives a high adsorption of 164.70 cc/g of CH4 (11.7 wt %) at 303 K and 60 bar. The channel surfaces decorated with -NH2 group and unsaturated metal centers in 1Cu' allow a promising 36.4 wt % of CO2 adsorption at 1 bar and 273 K. Moreover, it exhibits pronounced selectivity of CO2 adsorption over N2 and H2 at 273 K. Finally, the versatility of 1Cu' is shown by its excellent heterogeneous catalytic activity in the Biginelli coupling reactions involving an aldehyde, urea, and ethylacetoacetate to afford dihydroprimidinones.

l-Proline has been covalently attached in a rigid linear ligand, H4L, having an isophthalate moiety at each terminal to form the chiral ligand, H4LPRO. This linker has been used for the construction of a porous MOF, LCuPRO. The free l-proline moiety in the cavity of the framework in the presence of imidazole as a cocatalyst functions synergistically to catalyze the Baylis-Hillman reaction between α,β-unsaturated carbonyl compounds and aromatic aldehydes. High porosity of the framework is proven by the nitrogen adsorption isotherm.