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Background: Palatal rugae are elevations found on the anterior part of the hard palate and are the most stable features in the mouth. Our study aimed to analyze the palatal rugae pattern among an adolescent sample of the Albanian population in southeastern Kosovo. Methods and Results: A total of 100 adolescents (50 females and 50 males) aged from 12 to 18 were selected from schools in southeastern Kosovo. Palatal impressions were taken with elastomers, and models of the upper jaw were poured into stone casts for further examination. The rugae patterns were classified according to the Thomas and Kotze classification. The current study indicates that the predominant rugae pattern among the young population in southeastern Kosovo is the straight rugae. The predominant rugae pattern among female subjects is straight rugae and among males, wavy rugae; the frequency of various rugae patterns differed significantly between men and women. The total number of palatal rugae in men was significantly higher than in women. At the same time, there is no significant difference in the number of palatal rugae on the left and right sides between male and female subjects.

Background: Palatal rugae, or plicae palatinae, are uniquely designed structures, well-formed, asymmetric, irregular mucosal folds suited in the anterior part of the hard palate. Palatoscopy, or the analysis of the palatal rugae pattern, is a simple, low-cost, non-invasive, innovative, and highly sensitive technique that can be successfully used in stomatology. This study aimed to analyze the palatal rugae pattern among an adolescent sample of the Albanian population in Kosovo, to determine the most prevalent palatal rugae pattern in both genders in association with Class I, II, and III malocclusions according to Angle’s classification. Materials and Methods: In this cross-sectional study, a total of 100 adolescents (50 males and 50 females) aged from 12 to 18 were randomly selected from schools in southeastern Kosovo. All subjects were divided into classes of malocclusion according to Angle’s classification (Class I, Class II, and Class III). The rugae patterns were classified based on shape, unification, and length according to the Thomas and Kotze classification. In the present study, the palatal rugae pattern in Class I, II and III malocclusions show no significant difference between female and male subjects. In Class I malocclusion, the straight pattern was dominant in female subjects, and the wavy pattern was dominant in male subjects. The straight pattern was dominant in males and females with Class II malocclusion. In Class III malocclusion, the wavy pattern was dominant in female subjects, and the curved pattern was dominant in male subjects. The study showed that male subjects were at slightly higher risk for having Class I malocclusion and slightly lower risk for having Class II and Class III malocclusions than female subjects. Conclusion: This study provides essential information regarding the dominant palatal rugae pattern among Albanian adolescents of southeastern Kosovo with Class I, II, and III malocclusions according to Angle’s classification.

Background: Palatal rugae are asymmetrical elevations present on hard palate and are significant due to their uniqueness, stability and thus, aid in forensic identification. Objective: The objective is to determine palatal rugae morphology between parents and their offsprings and to determine their genetic inheritance. Materials and Methods: This randomized controlled cross-sectional single-blinded study was conducted on 50 families (randomly selected) who visited Vyas Dental College and Hospital. A total of 150 maxillary study models were examined and rugae were delineated and shape, size, and pattern of rugae were recorded. Results: Weak but significant correlation was seen in the nonspecific and angular type of palatal rugae between child and mother and child and father as P < 0.05. Matching with either or both of their parent was seen for curved, wavy, divergent, straight, horseshoe, angular, and nonspecific type of rugae with the higher % of wavy pattern matching (52% with mother and 54% with father). A considerable number of samples showed high heritability of primary and secondary rugae from both parents, but more predominantly to their mothers (branched, nonspecific, and straight). Conclusion: Both environmental and genetic factors can influence the rugae pattern. Further, studies should be conducted on a larger sample size to get more significant results.

Objective: Palatal rugae are frequently used in the evaluation of tooth movement after treatment in orthodontics and as a stable region in superimposition. It is important to note that the impression method and material used to record the rugae region affect the accuracy of the impression. The aim of this study is to compare the accuracy of palatal rugae, in three-dimensional (3D) by employing both conventional and digital impression methods. Materials and methods: In this study, 22 patients (12 females, 10 males) mean age of 13.5 ± 1.7 years old were selected with complete permanent dentition. Three different impressions were taken from the maxillae of the patients: conventional impression using silicone rubber impression material, conventional impression using alginate impression material, and optical impression using an intraoral scanner. The impressions’ digital data were analyzed by the GOM Inspect (Version 2018, Braunschweig, Germany), a 3D analysis software. The Root Mean Square (RMS) values of the total ruga region were evaluated in this software. The data were statistically analyzed using the Jamovi program. The Kruskal–Wallis test and Mann–Whitney U test were performed due to the non-normal distribution of the data. Results: There is no statistically significant difference between the comparison points of the right and left rugae’s medial and lateral points and total rugae regions’ RMS values. Although there was no statistically significant difference, the total RMS values of alginate and digital scan measurements showed closer results than the RMS values of silicone and digital scan measurements. Conclusion: The study found that there was no statistically significant difference in the total RMS values of the ruga region between traditional and digital impression methods. Clinical relevance: The treatment period in orthodontics is long. Different impression materials and methods can be used for diagnostic, mid-treatment, and final impressions. For superimpositions and treatment and post-treatment palatal ruga evaluations, traditional and digital impression methods are clinically acceptable and can be used as alternatives to each other. [ABSTRACT FROM AUTHOR]

Background: Palatal rugae are asymmetrical elevations present on hard palate and are significant due to their uniqueness, stability and thus, aid in forensic identification. Objective: The objective is to determine palatal rugae morphology between parents and their offsprings and to determine their genetic inheritance. Materials and Methods: This randomized controlled cross-sectional single-blinded study was conducted on 50 families (randomly selected) who visited Vyas Dental College and Hospital. A total of 150 maxillary study models were examined and rugae were delineated and shape, size, and pattern of rugae were recorded. Results: Weak butsignificant correlation wasseen in the nonspecific and angular type of palatal rugae between child and mother and child and father as P < 0.05. Matching with either or both of their parent was seen for curved, wavy, divergent, straight, horseshoe, angular, and nonspecific type of rugae with the higher % of wavy pattern matching (52% with mother and 54% with father). A considerable number of samples showed high heritability of primary and secondary rugae from both parents, but more predominantly to their mothers (branched, nonspecific, and straight). Conclusion: Both environmental and genetic factors can influence the rugae pattern. Further, studies should be conducted on a larger sample size to get more significant results. [ABSTRACT FROM AUTHOR]

Recent advances in imaging technologies, such as intra‐oral surface scanning, have rapidly generated large datasets of high‐resolution three‐dimensional (3D) sample reconstructions. These datasets contain a wealth of phenotypic information that can provide an understanding of morphological variation and evolution. The geometric morphometric method (GMM) with landmarks and the development of sliding and surface semilandmark techniques has greatly enhanced the quantification of shape. This study aimed to determine whether there are significant differences in 3D palatal rugae shape between siblings. Digital casts representing 25 pairs of full siblings from each group, male–male (MM), female–female (FF), and female–male (FM), were digitized and transferred to a GM system. The palatal rugae were determined, quantified, and visualized using GMM computational tools with MorphoJ software (University of Manchester). Principal component analysis (PCA) and canonical variates analysis (CVA) were employed to analyze palatal rugae shape variability and distinguish between sibling groups based on shape. Additionally, regression analysis examined the potential impact of shape on palatal rugae. The study revealed that the palatal rugae shape covered the first nine of the PCA by 71.3%. In addition, the size of the palatal rugae has a negligible impact on its shape. Whilst palatal rugae are known for their individuality, it is noteworthy that three palatal rugae (right first, right second, and left third) can differentiate sibling groups, which may be attributed to genetics. Therefore, it is suggested that palatal rugae morphology can serve as forensic identification for siblings. [ABSTRACT FROM AUTHOR]

Background: Sickle cell disease (SCD) is the most common hereditary hemoglobinopathy, which delays growth leading to an altered skeleton and craniofacial pattern. Palatal rugae patterning has been considered the regulator of the development of the palate. The purpose of the research work was to study the morphology of the palate, rugae pattern, and its dimensions in SCD children and compare them with healthy normal children, and to evaluate its role as minor physical anomalies (MPAs). Methods: A cross-sectional case-control study was designed as per STROBE guidelines. The sample comprised 50 children diagnosed with sickle cell disease (Group SCD) and 50 normal healthy children as control (Group C) belonging to the same age group (10–18 years). Dental impressions were made, followed by the pouring of dental casts. The length of the palatal rugae was measured and categorized into primary (>5 mm), secondary (3 mm–5 mm), and fragmentary rugae (

The purpose of this study is a new method that can help to identify the sex through the study of palatal rugae, comparing sagittal sections of the hard palate using Cartesian coordinates and evaluating the assistance given by digital technology and its applicability in this method. In this study, 57 digital impressions were examined and divided in two groups based on sex. Results: 2223 impression sections were studied and 145 coordinates that were present with a frequency greater than 50% in one or both groups were obtained: 52 discriminating traits (DT) in the male group, 29 discriminating traits in the female group, and 64 common traits (CT). The DTs in the female group showed no statistically significant difference from the same coordinates in the male one (p = 0.832). Statistically significant differences were observed in the DTs in the male group compared to the same coordinates in the female group (p = 0.018). No statistically significant differences were observed in the frequency of DTs in both sexes (p = 0.056). Further research in forensic odontology is needed to determine its scientific certainty. It is certain that digital technology may one day be a valuable support for the forensic odontologist but to date the lack of dedicated and certified programs limits its reliability. [ABSTRACT FROM AUTHOR]

Abstract Background Because of the individuality of the palatal rugae, they are applicable for human identification purposes the same as the fingerprints. Therefore, it is important to assess the effect of different factors on rugae characteristics. We assessed the characteristic changes of the palatal rugae following extraction and expansion during orthodontic treatments. Before and after orthodontic treatment dental casts of 30 patients were scanned by 3Shape Trios 3D intraoral scanner, and superimposed in Geomagic X 2018 software. Displacement of 13 reference points was analyzed in three dimensions. The length of the palatal rugae was also measured. SPSS version 22.0 was applied for data analysis, using independent t test, sample t test, and chi-square test. Results The alterations in the mean palatal rugae length were significant in the first right and third right ridges after extraction, and right and left second ridges after expansion treatment (P < 0.05). Changes in coordinates in the X, Y, and Z axes were significant at 8 points on the rugae after extraction, and 5 points after expansion treatment (P < 0.05). The changes in the sum of means of displacements were significant at all 13 points after extraction and expansion treatments (P < 0.05). Conclusions The palatal rugae do not remain stable during expansion and extraction orthodontic treatments. Some changes occurred in length, and the most medial and most lateral points on each ridge were displaced. The length of the incisive papilla did not change during treatment; thus, it can serve as a stable landmark. If we consider palatal rugae as an individual landmark, we can refer the patients after orthodontic treatment for scanning of their palatal rugae to document their post treatment pattern; but it should be noted that treatment relapse may change it again.

Background: In post-mortem scenarios, often it is a very difficult process to establish a person's identity. Rugae are unique in that they are protected from trauma as they are insulated from heat by tongue and buccal pad of fat unlike fingerprint or lip print that is prone to destruction. Aim and Objectives: This study was aimed to compare the palatal rugae among people of different races. The sole objectives of the study were to assess the predominant pattern in the selected groups, reliability of rugae pattern in personal identification, to evaluate reliability of sex determination and to compare the total number of rugae on right and left sides of the palate among the males and females. Study Design: A total of 90 subjects were enrolled into the study and divided into three groups that are African, Dravidian and Mongoloid population. Shapes of rugae present were analyzed according to the classification given by Kapali et al. (1997) and Thomas & Kotze (1983). Result: The predominant rugae shape in African and Dravidian population was wavy pattern, whereas Mongoloid race was predominant in curve pattern. African and Dravidian males were predominant in wavy pattern when compared to Mongoloid males where unification type was more predominant. Conclusion: A statistically significant association between the rugae shape in three populations exists, although subtle yet definite.

A sample of 38 Italian subjects (17 males and 21 females, mean age 8.2 ± 1.2 years, age range 7.2–8.8 years), who underwent active Rapid Maxillary Expansion (RME), was studied to assess the reliability of an intraoral scan and the stability of palatal rugae. An intraoral scanner was used to obtain digital dental models pre- and post-treatment. A 3D-3D superimposition procedure was performed, and the Root Mean Square (RMS) of the distance calculated, to verify the forensic validity of this biometric technique. The Intra-Class Correlation Coefficient (ICC) values for the overall inter- and intra-observer agreement were 0.955 (95% CI: 0.931–0.980; p < 0.001) and 0.936 (95% CI: 0.918–0.975; p < 0.001), respectively. The values for TEM and rTEM tests were 0.423 and 1.2% for intra-observer; 0.637 and 1.7% for inter-observer. Results showed a statistically significant difference in RMS mean point-to-point distance between untreated control and treated subjects (p = 0.002). The RMS values to distinguish the pooled group (untreated and treated with RME) from any mismatch ranged from 0.42 to 0.61. This methodology was highly suitable to detect changes caused by RME. Since some modifications were detected, caution is necessary when palatal rugae are analysed for forensic purposes in subjects who underwent RME. [ABSTRACT FROM AUTHOR]

Background: In post‑mortem scenarios, often it is a very difficult process to establish a person’s identity. Rugae are unique in that they are protected from trauma as they are insulated from heat by tongue and buccal pad of fat unlike fingerprint or lip print that is prone to destruction. Aim and Objectives: This study was aimed to compare the palatal rugae among people of different races. The sole objectives of the study were to assess the predominant pattern in the selected groups, reliability of rugae pattern in personal identification, to evaluate reliability of sex determination and to compare the total number of rugae on right and left sides of the palate among the males and females. Study Design: A total of 90 subjects were enrolled into the study and divided into three groups that are African, Dravidian and Mongoloid population. Shapes of rugae present were analyzed according to the classification given by Kapali et al. (1997) and Thomas & Kotze (1983). Result: The predominant rugae shape in African and Dravidian population was wavy pattern, whereas Mongoloid race was predominant in curve pattern. African and Dravidian males were predominant in wavy pattern when compared to Mongoloid males where unification type was more predominant. Conclusion: A statistically significant association between the rugae shape in three populations exists, although subtle yet definite. [ABSTRACT FROM AUTHOR]

Background: Because of the individuality of the palatal rugae, they are applicable for human identification purposes the same as the fingerprints. Therefore, it is important to assess the effect of different factors on rugae characteristics. We assessed the characteristic changes of the palatal rugae following extraction and expansion during orthodontic treatments. Before and after orthodontic treatment dental casts of 30 patients were scanned by 3Shape Trios 3D intraoral scanner, and superimposed in Geomagic X 2018 software. Displacement of 13 reference points was analyzed in three dimensions. The length of the palatal rugae was also measured. SPSS version 22.0 was applied for data analysis, using independent t test, sample t test, and chi-square test. Results: The alterations in the mean palatal rugae length were significant in the first right and third right ridges after extraction, and right and left second ridges after expansion treatment (P < 0.05). Changes in coordinates in the X, Y, and Z axes were significant at 8 points on the rugae after extraction, and 5 points after expansion treatment (P < 0.05). The changes in the sum of means of displacements were significant at all 13 points after extraction and expansion treatments (P < 0.05). Conclusions: The palatal rugae do not remain stable during expansion and extraction orthodontic treatments. Some changes occurred in length, and the most medial and most lateral points on each ridge were displaced. The length of the incisive papilla did not change during treatment; thus, it can serve as a stable landmark. If we consider palatal rugae as an individual landmark, we can refer the patients after orthodontic treatment for scanning of their palatal rugae to document their post treatment pattern; but it should be noted that treatment relapse may change it again. [ABSTRACT FROM AUTHOR]

The purpose of this study is a new method that can help to identify the sex through the study of palatal rugae, comparing sagittal sections of the hard palate using Cartesian coordinates and evaluating the assistance given by digital technology and its applicability in this method. In this study, 57 digital impressions were examined and divided in two groups based on sex. Results: 2223 impression sections were studied and 145 coordinates that were present with a frequency greater than 50% in one or both groups were obtained: 52 discriminating traits (DT) in the male group, 29 discriminating traits in the female group, and 64 common traits (CT). The DTs in the female group showed no statistically significant difference from the same coordinates in the male one (p = 0.832). Statistically significant differences were observed in the DTs in the male group compared to the same coordinates in the female group (p = 0.018). No statistically significant differences were observed in the frequency of DTs in both sexes (p = 0.056). Further research in forensic odontology is needed to determine its scientific certainty. It is certain that digital technology may one day be a valuable support for the forensic odontologist but to date the lack of dedicated and certified programs limits its reliability.

Palatal Rugae (PR) are disproportionate bilateral protuberances of connective tissue found on each side of the medpalatine raphe posterior to the incisive papilla. The aim of this study was to investigate the pattern and sexual dimorphism of PR. The prevalence and pattern of PR of 120 Portuguese individuals attending the Orthodontic Clinic of Egas Moniz were categorized. The sample comprised 44.2% males and 55.8% females with similar PR frequencies among sex and age. The total number PR was 624. Straight and wavy PR morphologies were the most frequently observed. There was no sexual dimorphism related to the PR pattern.

Abstract Background The purpose of the cross sectional study was to investigate the distal extension of the rugae area in a Jordanian (Middle Eastern) population, as an anatomical limitation influencing the surgical decision of harvesting a palatal soft tissue graft. Factors that may influence or predict the extension were also assessed. Methods Sixty periodontally healthy participants (29 males and 31 females) were included. Maxillary alginate impressions were made and casts were poured. The measurements were highlighted from the origin of the rugae (near mid palatine raphe) to the terminal end with a sharp graphite pencil on the cast and a magnification lens was used for identification. The most posterior extension of the rugae were marked on the casts and determined by a standardized periodontal probe. The normal approximation test for binomial distribution was used to determine the proportion of the subjects with rugael extensions beyond the mesial end of the upper second premolar and fisher’s exact test for the purpose of analysis of the association of this extension with other factors. Results In almost half (41.7%) of the sample, the rugae extended distal to the upper second premolar, 23.3% extended to the mid-palatal of the upper second premolar, and 11.7% extended to the mesial of the upper second premolar. The implication is that 90.0% of the rugae reached the upper second premolar and 78.3% extended beyond its mesial aspect. The normal approximation test performed with 95% CI with the "rugael extension proximal to the mesial end of the upper 2nd premolar" considered to be the "success" category revealed that the proportion of the subjects with rugael extensions proximal to the mesial end of the upper second premolar was significantly lower than the proportion beyond the mesial end of the upper second premolar (95% CI of 11.2–32.0%, p = .00001). There was no significant difference between gender, smoking status, gingival phenotype and palatal shape with the posterior extension of palatal rugae. Conclusions Palatal rugae in a sample of a Jordanian population extends beyond the mesial aspect of the upper second premolar which may cause a substantial limitation for graft harvesting from the palate. The hard palate of Jordanian patients may not be a reliable source of soft tissue grafts required for aesthetic mucogingival surgery. No significant association existed between the most posterior extent of palatal rugae and gender, gingival phenotype or palatal shape. Other possible sources should be explored.

Aim: To determine the correlation between palatal rugae (PR) pattern and subjects with vertical, average, and horizontal growth pattern and to determine gender variation if any. Material and Methods: One hundred and twenty study models were divided into horizontal, vertical, and average growth patterns with 40 (20 males and 20 females) samples in each group. The pattern of PR was assessed in each group using a template. Result: Type B (wavy type) PR occurs more often in horizontal and average growth pattern (80% and 72.5% respectively), whereas its occurrence in vertical growth pattern subject is the least among other growth patterns, that is, 42.5% of the total subjects. Type E (curved type) PR has appeared highest in 27.5% of average growth pattern patient followed by 12.5% of vertical growth pattern subjects. The occurrence of type E PR is least in horizontal growth pattern which is just 5% of the total subjects. Conclusion: Curved-type PR was more prevalent in vertical and average growth patterns. Diverging PR pattern was mainly seen in vertical growth pattern. PR also shows sexual dimorphism. The association is clinically significant as it will lead to determination of facial vertical growth pattern at early age; thus, it serves as a diagnostic adjunct for different malocclusions very early in life and aids in preventive or interceptive orthodontic measures to alleviate forthcoming growth pattern. [ABSTRACT FROM AUTHOR]

Komal Smriti,1 Raj Gupta,2 Kalyana C Pentapati,3 Anupam Singh,4 Ira Kapoor,2 Ravindranath Vineetha,1 Srikanth Gadicherla4 1Department of Oral Medicine and Radiology, Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Manipal, India; 2Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Manipal, India; 3Department of Public Health Dentistry, Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Manipal, India; 4Department of Oral and Maxillofacial Surgery, Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Manipal, IndiaCorrespondence: Kalyana C PentapatiDepartment of Public Health Dentistry, Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, IndiaEmail drkalyan81@gmail.comBackground: Palatal rugae are asymmetric and irregular ridges on the anterior palatal region, which have proven to be useful in the field of forensic dentistry. This study aims to use morphological analysis of palatal rugae patterns to assess sex in a South Indian adult population.Materials and Methods: This cross-sectional study was undertaken to evaluate a total of 300 maxillary dental casts (150 males and 150 females). The rugae patterns were marked by one observer and classified as per Thomas and Kotze criteria by another observer. The rugae patterns were classified based on the total number of rugae, shape, predominant direction of rugae, and unification of rugae.Results: The palatal rugae in female participants showed a higher incidence of circular rugae and backward directed rugae, while male participants showed more forward-directed rugae. The logistic regression analysis showed a significant association of with circular (OR=1.48; 95% CI= 1.02– 2.13), forward (OR=0.87; 95% CI= 0.78– 0.97) and backward (OR=1.23; 95% CI= 1.08– 1.41) palatal rugae with sex.Conclusion: Certain palatal rugae patterns can be used for the assessment of sex with limited accuracy.Keywords: palatal rugae, sex, identification, palatoscopy, forensic, dentistry

Palatal rugoscopy is the use of palatal rugae for identification of unknown persons. The majority of the population in Egypt consists of native Egyptian people. However, some Malaysian people live in Alexandria city of Egypt for the purpose of education. So, in case of mass disaster, there is a critical need for a reliable and easy method to differentiate between Malaysians and Egyptians. This study aimed to determine the palatal rugae patterns in two diverse populations; Egyptians and Malaysians and its relation to sex and population difference. Eighty students of Alexandria Faculty of Dentistry; forty Egyptians (20 females and 20 males) and forty Malaysians (20 males and 20 females). The age ranged 18-30 years. Impressions were taken with alginate (irreversible hydrocolloid impression material). Thomas and Kotze classification was used to assess palatal rugae regarding their length, shape, direction and unification. Data were introduced to the computer and processed using IBM SPSS software version 20.0. Qualitative data were defined using number and percent. The Kolmogorov-Smirnov test was applied to attest the normality of distribution. Significance of the obtained results was judged at the 5% level. Significant difference was noted between Malaysian males and females according to total number of rugae on both sides (t= 2.210 and p= 0.033). Malaysians had significantly higher total rugae number on both sides than Egyptians. Curved shape was the predominant shape in the total sample in the four studied groups (42.9%, 36.3%, 46.8% and 44.1%). Significant difference was detected between Egyptians and Malaysians according to the predominant palatal rugae direction on both left and right sides (x2 = 6.293 and p=0.043) (x2 =6.620 and p=0.037) respectively. Egyptian females had significantly higher percentage of absent unification than Egyptian males. Binary logistic regression models for sex and ethnicity identification were built up.

Background: Palatal rugae are unique for an individual and remain unchanged during individual's lifetime. Application of palatal rugae analysis as a method in gender identification needs to be confirmed with more scientific studies. The objectives of the present study were to assess the distribution of the palatal rugae patterns in Kerala population and compare the distribution of these parameters between males and females. Materials & Methods: A retrospective cross-sectional study was conducted by evaluating the casts of 100 dentate individuals, between the age of 18 and 30 years. The rugae were divided into two halves and patterns were then assessed as per Thomas and Kotze classification. Independent sample t-test was used to assess the significant difference of each type of palatal rugae between males and females. Results: Wavy rugae (mean – 5.6 ± 0.7) was predominant among males whereas curved (mean – 4.6 ± 0.6) and circular rugae (mean – 0.9 ± 0.3) were more in females, and were found to be statistically significant (p

Background: Palatal rugae are unique for an individual and remain unchanged during individual's lifetime. Application of palatal rugae analysis as a method in gender identification needs to be confirmed with more scientific studies. The objectives of the present study were to assess the distribution of the palatal rugae patterns in Kerala population and compare the distribution of these parameters between males and females. Materials & Methods: A retrospective cross-sectional study was conducted by evaluating the casts of 100 dentate individuals, between the age of 18 and 30 years. The rugae were divided into two halves and patterns were then assessed as per Thomas and Kotze classification. Independent sample t-test was used to assess the significant difference of each type of palatal rugae between males and females. Results: Wavy rugae (mean – 5.6 ± 0.7) was predominant among males whereas curved (mean – 4.6 ± 0.6) and circular rugae (mean – 0.9 ± 0.3) were more in females and were found to be statistically significant (p<0.05). The mean number of secondary rugae in females (1.5 ± 0.5) was greater than that of males (1.3 ± 0.4) and was found to be statistically significant (p<0.05). The diverging type was commonly found in females compared to males. Conclusion: Our findings showed statistically significant differences in the shapes and patterns of rugae between males and females among Kerala population. Thus, morphological analysis of palatal rugae can be considered as an additional aid in forensic odontology for gender differentiation. [ABSTRACT FROM AUTHOR]

Objective Palatal rugae are anatomically asymmetrical and irregular ridges in the anterior palatal region. The palatal ruages were demonstrated to be useful in the field of forensic dentistry. This study was taken to assess the ruage patter to compare with gender identification in those who had attended dental institutions in Andhra Pradesh. Materials and Methods A total of 40 subjects were included in the present study, those who attended a dental institution as a prospective cross sectional study. The rugae patterns were marked and classified according to Kapali et al., 1997, based on morphological pattern. Results Female participants were more numerous in study samples when compared with males. The palatal ruage in female participants showed a higher incidence of wavy, circular, and divergent patterns, while male participants showed straight, curved, wavy, and convergent rugae patterns. Comparison of gender with respect to the mean number of rugae patterns on the right and left sides using a student t-test revealed that there was no stastically significant (p≥0.05). Summary and Conclusions The rugae pattern can be one of the important aspects in the field of forensic odontology. The palate represents a suitable landmark for unique and identifying features, with the additional benefit of survival in most postmortem events that can disrupt or change other body parts. [ABSTRACT FROM AUTHOR]

Objective To examine the method of least-square fitting for calibrating the palatal rugae boundary. Methods According to the distribution of the teeth, the feature points were selected; when they were fit, the boundary of the palatal rugae area was created, thereby constructing a mask. The mask was used to remove the part located outside the boundary and filter out the interference. Three samples were utilized for the experiments and analyses to come. Results To evaluate the quantitative results of the fitting curves, the correlation coefficients（r）of the samples and the relationship between the actual mean value and ideal mean value was obtained through six fitting processes. The differences between the actual mean and ideal mean were found to be significantly small（from 0.285 7 to 2.500 0）in the six fitting processes, with the range of r close to 1（from 0.989 6 to 0.999 5）. Conclusion The effect of the cubic polynomial fitting method adopted in this study was stable.The proposed boundary calibration method can effectively locate the palatal rugae boundary and remove the interference area, further promoting the practice of forensic identification.

Abstract Background DNA base identification is a proper and high specificity method. However, identification could be challenged in a situation where there is no database or the DNA sequence is almost identical, as in the case of monozygotic (MZ) twins. The aim of this study was to introduce a novel forensic method for distinguishing between almost identical MZ twins by means of an intraoral scanner using the 3D digital pattern of the human palate. Methods The palatal area of 64 MZ twins and 33 same-sex dizygotic (DZ) twins (DZSS) and seven opposite-sex dizygotic twins (DZOS) were scanned three times with an intraoral scanner. From the scanned data, an STL file was created and exported into the GOM Inspect® inspection software. All scans within a twin pair were superimposed on each other. The average deviation between scans of the same subject (intra-subject deviation, ISD) and between scans of the two siblings within a twin pair (intra-twin deviation, ITD) was measured. One-sided tolerance interval covering 99% of the population with 99% confidence was calculated for the ISD (upper limit) and the ITD (lower limit). Results The mean ISD of the palatal scan was 35.3 μm ± 0.78 μm. The calculated upper tolerance limit was 95 μm. The mean ITD of MZ twins (406 μm ± 15 μm) was significantly (p

Background: The palatal rugae contribute to oral swallowing, taste perception, and speech. From a forensic point of view, the distinctive morphology of these structures can be used to support human identification. However, the morphology of the rugae may be altered by trauma or therapeutic interventions in the palate. Objective: This study aimed to analyze the morphology of the palatal rugae before and after orthodontic treatment performed with and without maxillary expansion and dental extractions. Materials and Methods: The sample consisted of 60 dental casts taken before (n = 30) and after (n = 30) orthodontic treatment from patients aged >18 years. The patients were treated with rapid maxillary expansion by using Haas appliance (n = 10), by extracting the maxillary first premolars (n = 10) and by using only conventional fixed orthodontic appliances (n = 10). All the dental casts were analyzed twice by two independent examiners that were blind for the type of treatment. Results: All the differences between groups were statistically significant (P < 0.05). More alterations in the morphology of the palatal rugae were observed in patients treated with rapid maxillary expansion, whereas few alterations were performed in patients treated with no maxillary expansion or dental extractions. Conclusion: The use of palatal rugae for forensic purposes must be avoided in patients that underwent invasive orthodontic treatments, such as those founded on maxillary expansion and dental extractions.

Objectives: Whenever there is a loss of a tooth, palatal rugae (PR) is suggested as an alternative technique for personal identification. The present study was carried to determine area-specific uniqueness of the rugae pattern among the population of Mewar and Hadoti region by evaluating the number and shape of the rugae pattern and its variation among genders of the same population. Materials and Method: Study included 500 subjects (250 males and 250 females) aged between 17 and 25 years as per the inclusion and exclusion criteria. The rugae pattern was classified based on their length, shape, direction, and unification proposed by Lysell (1955) and later modified by Thomas and Kotze (1983). The observations recorded were evaluated for their statistical significance and specificity using unpaired t-test. Results: Total number of rugae on the right side in both genders was 2089 showing a significant difference between males and females and on the left side, the total number was 2088 not showing a statistically significant difference between genders. The wavy pattern was the most predominant pattern, followed by curved and straight patterns. The wavy pattern was found to be significant between males and females. Conclusion: The present study demonstrated the difference in PR distribution and uniqueness in different individuals and the importance of studying the characteristics based on ethnicity. Also, we can say that gender can be determined using a wavy pattern.

Introduction Palatoscopy or palate rugoscopy has been studied over the years to identify its unique characteristics to utilize for the human identification process. The unique anatomical location of the palatal rugae provides stability which along with the uniqueness of the palatal rugae pattern can contribute in human identification. Despite its simplicity and reliability, the palatal rugae is unexplored in the Nepalese population. The objective of the present study is to identify the rugae pattern and assess its sexual dimorphism. Methods This was a cross-sectional analytical study conducted in Tribhuvan University Dental Teaching Hospital (TUDTH). The characteristics of palatal rugae based upon number, length, shape, and unification were analyzed on 100 dental casts. The rugae patterns were marked with a black color graphite pencil under adequate illumination. The length of the rugae was measured with the help of a digital vernier caliper and brass wire. The independent sample T-test and Mann Whitney U tests were used to assess the sexual dimorphism. Results The predominant type of rugae according to the size in both males and females was primary rugae. According to the shape of the rugae, the predominant type of rugae was wavy followed by curved, straight, diverging, converging, and circular in both the sexes. However, no significant difference was seen in the rugae pattern in males and females. Conclusion Based on the findings of the present study, the use of palatal rugae for sex determination is not recommended. [ABSTRACT FROM AUTHOR]

Objective: To assess the topographic changes in palatal rugae in dentate and edentulous subjects and explore the topographic changes that has occurred due to aging and tooth loss. Study Design: A cross sectional observational study. Place and Duration: At Prosthodontic department of Lahore Medical and Dental College, Lahore from 5th January 2021 to 5th April 2021. Methodology: A total of 40 participants, (20 dentate; age ranged 20-30 years, 20 edentulous; age ranged 50-70 years) were selected using non probability purposive sampling. Demographic data was collected and intra oral examination was done. Alginate impressions were taken and maxillary casts were fabricated. The casts were minutely analyzed for number, direction, shape, length and unification of rugae. Sharp graphite pencil was used for marking and Modified Thomas and Kotze classification was used to examine rugae characteristics. Results: Topographic change was seen in rugae patterns and their distribution varies in both groups. The number of primary rugae was higher in dentate as compare to edentulous group (8.10 ± 0.788; 5.00 ± SD 1.75). The secondary rugae's count was also a bit higher in dentate group as compared to edentulous (2.00 ±SD 0.918, 0.65 ± SD 0.489). The commonest pattern seen in dentate group is more complexed i.e., wavy (2.80 ± 0.616), whereas in edentulous group the commonest pattern observed was straight rugae (2.78 ±SD 1.6). Dentate group had more frequent forward directed rugae (2.80 ± SD 1.196; 1.62 ±SD 0.885) as compared to edentulous group where perpendicular directed (2.50 ±SD 1.39) were more frequent. Significant difference was seen in convergent pattern of rugae of both groups (dentate .90 ±SD 1.02, edentulous 1.00 ±SD.001). Significant statistical difference was found in both groups with respect to number, direction, shape unification and lengths; p < 0.05. Conclusion: The aging process and tooth loss altered the topographical characteristics of palatal rugae and resulted in less number and complexity of characteristics in edentulous subjects as compared to dentate. [ABSTRACT FROM AUTHOR]

Objective: The Hedgehog pathway is a fundamental signaling pathway in organogenesis. The expression patterns of the ligand Sonic Hedgehog (Shh) and key pathway components have been studied in many tissues but direct spatial comparisons across tissues with different cell compositions and structural organization are not common and could reveal tissue-specific differences in pathway dynamics. Results: We directly compared the expression characteristics of Shh, and four genes with functional roles in signaling and whose expression levels serve as readouts of pathway activity in multiple tissues of the embryonic mouse head at embryonic day 15.5 by serial in situ hybridization. The four readout genes were the positive feedback regulator Gli1, and three negative feedback regulators, Patched1, Patched2, and Hedgehog Interacting Protein. While the relative abundance of Gli1 was similar across tissues, the relative expression levels and spatial distribution of Shh and the negative feedback regulators differed, suggesting that feedback regulation of hedgehog signaling is context dependent. This comparative analysis offers insight into how consistent pathway activity could be achieved in tissues with different morphologies and characteristics of ligand expression. [ABSTRACT FROM AUTHOR]

Objectives: To investigate the anteroposterior and vertical changes of the median rugae area, which is commonly used as dental model superimposition reference, relevant to its underlying skeletal structures.Settings and Sample Population: Retrospectively collected pre- and post-treatment cephalometric radiographs and 3D digital dental models of 24 orthodontic patients (age at treatment start: 12.26 ± 0.83 years; assessment period: 2.13 ± 0.68 years) were analysed. All had mild to moderate malocclusions that were treated non-extraction with full fixed appliances.Material and Methods: The incisive papilla and rugae points were placed on the dental models that were then registered to the cephalometric radiographs. Afterwards, the radiographs were superimposed on Sella, ANS-PNS, and through a maxillary structural method. The vertical and horizontal movements of the papilla and the rugae points, as well as of a central incisor, were measured (Viewbox 4 software).Results: The incisive papilla and the three rugae points remained stable anteroposteriorly, but moved downwards in the vertical dimension (approximately 1-2 mm), in a similar manner (P > .05). However, the anteroposterior position of the papilla and the first rugae points were affected by changes in anterior tooth position and inclination (P < .05).Conclusion: Both the second and third rugae can be used as superimposition references for tooth movement assessment. The use of the papilla and the first rugae area is not recommended, because they are affected by tooth movement. The outcomes of a palatal superimposition are comparable to those of a maxillary skeletal superimposition in the anteroposterior, but not in the vertical dimension. [ABSTRACT FROM AUTHOR]

The study of palatal rugae has an important role in establishing personal identity and facilitating population identification. The current work analyzed the pattern of palatal rugae among an adult Jordanian population and searched for any gender differences. A total of 100 random dental casts were included in this study. Number and pattern of palatal rugae regarding length, shape, direction, and unification of rugae were identified. In addition, measurement of maxillary intercanine widths (MIW) using digital Vernier caliper was done. Palatal rugae on the right side outnumbered those on the left side. Males had more rugae in general than females. The commonest rugae in both sexes were serpiginous. Primary and forward rugae showed the highest frequency. A diverging form of unification was more prevalent than a converging form. Apart from a total number of convergent, divergent and secondary rugae, the total number of other rugae differs significantly between males and females. The study showed characteristics of palatal rugae in a Jordanian population which could be gender specific, facilitating their identification. It revealed the presence of sexual dimorphism, and sex can be predicted using either logistic regression equation or CART model.

Objectives: To evaluate the individuality of palatal rugae patten and correlate them in males and females in different age groups by using Trobo's classification. Study Design: Cross Sectional. Setting: Department of Prosthodontics, Dow Dental College, Dow University of Health Sciences. Period: September 2019 to January 2020. Material & Methods: This study comprised of ninety-four participants with equal number of males and females from 10 years to 70 years, visiting the Prosthodontic department. Study casts included were free of dental abnormality, trauma, malocclusion, orthodontic problems, and deformity in palatal rugae area. Sample size was calculated from software name openepi.com with 95% confidence interval. Data was tabulated and SPSS version 22.0 was used to identify the results. Results: Frequency distribution was performed among age groups in which second group (20 to 39 years) has highest frequency with 52 in number. The cross tab between age group and type of rugae was made which showed group II, having age between 20 to 39 years, had the highest number of palatal rugae configurations amongst all. Conclusion: Sinuous or type E was the predominant type of palatal rugae found in this study. The study did not show significant difference in gender, however number of rugae were found consistently more in young adults with propensity towards individuality. Their use can thus be justified for dental identification in forensic odontology. [ABSTRACT FROM AUTHOR]

Background: The purpose of the cross sectional study was to investigate the distal extension of the rugae area in a Jordanian (Middle Eastern) population, as an anatomical limitation influencing the surgical decision of harvesting a palatal soft tissue graft. Factors that may influence or predict the extension were also assessed. Methods: Sixty periodontally healthy participants (29 males and 31 females) were included. Maxillary alginate impressions were made and casts were poured. The measurements were highlighted from the origin of the rugae (near mid palatine raphe) to the terminal end with a sharp graphite pencil on the cast and a magnification lens was used for identification. The most posterior extension of the rugae were marked on the casts and determined by a standardized periodontal probe. The normal approximation test for binomial distribution was used to determine the proportion of the subjects with rugael extensions beyond the mesial end of the upper second premolar and fisher's exact test for the purpose of analysis of the association of this extension with other factors. Results: In almost half (41.7%) of the sample, the rugae extended distal to the upper second premolar, 23.3% extended to the mid-palatal of the upper second premolar, and 11.7% extended to the mesial of the upper second premolar. The implication is that 90.0% of the rugae reached the upper second premolar and 78.3% extended beyond its mesial aspect. The normal approximation test performed with 95% CI with the "rugael extension proximal to the mesial end of the upper 2nd premolar" considered to be the "success" category revealed that the proportion of the subjects with rugael extensions proximal to the mesial end of the upper second premolar was significantly lower than the proportion beyond the mesial end of the upper second premolar (95% CI of 11.2–32.0%, p =.00001). There was no significant difference between gender, smoking status, gingival phenotype and palatal shape with the posterior extension of palatal rugae. Conclusions: Palatal rugae in a sample of a Jordanian population extends beyond the mesial aspect of the upper second premolar which may cause a substantial limitation for graft harvesting from the palate. The hard palate of Jordanian patients may not be a reliable source of soft tissue grafts required for aesthetic mucogingival surgery. No significant association existed between the most posterior extent of palatal rugae and gender, gingival phenotype or palatal shape. Other possible sources should be explored. [ABSTRACT FROM AUTHOR]

Introduction: Palatal rugae or plicae palatine are uneven ridges made of connective tissue situated beneath the incisive papilla. DNA analysis, finger prints analysis, are the most common techniques used for establishing person’s identity but in situation where using these techniques are difficult palatal rugae can be used as a second source of identification. However, some habits like prolonged thumb sucking and orthodontic treatment for long duration could affect the rugae pattern. Hence, this study aims to evaluate the changes in transverse dimensions of palatal rugae in subjects treated with rapid maxillary expansion Material and Methods: The palatal rugae patterns were measured and compared to evaluate the morphometric changes between pre-treatment cast (30 Pre-RME) and its post-treatment (30 Post-RME) counterpart. All pre-and post-expansion dental casts were traced and the intermedial and interlateral dimensions of first, second and third rugae were measured. The statistical analysis was done using paired t test. Conclusion: The medial aspect of first primary rugae can be considered as a stable reference landmark while the lateral and medial aspects of the 2nd and 3rd rugae are liable to changes in transverse dimension. Hence, for forensic identification the stability of palatal rugae in patients who had undergone rapid maxillary expansion still remains questionable. [ABSTRACT FROM AUTHOR]

Context: The study of palatal rugae forms an important basis of human identification, especially due to mass disasters where routinely used techniques may not be helpful. Aims: This study aimed at evaluating the palatal rugae and incisive papilla on the basis of shape. Settings and Design: The study was conducted in 280 individuals (males and females) among dental students of Dayananda Sagar College of Dental Sciences. Materials and Methods: The study included 280 students from Dayananda Sagar College and out-patients from the orthodontics department. Pictures of rugae and the incisive papilla were taken from individuals aged 10–36 using a camera, mirror, and lighting. Two investigators analysed the shape of the rugae and incisive papilla using classification systems by Thomas and Kotze, and Ortman and Tsao, respectively. Statistical Analysis Used: The data were statistically analysed using SPSS 20.0 software, and a significance level of P ≤ 0.05 was used. Results: The results suggested that rugae showing a wavy shape were the most common pattern in both the genders. Significant differences were observed in the curved rugae type between males and females. In incisive papillae evaluation, the pear shape was the most common, with the triangular shape being the least common. Conclusions: It can be concluded that evaluation of palatal rugae along with the incisive papilla can be an important tool for identification of an individual and for evaluating various ethnic populations. [ABSTRACT FROM AUTHOR]

Objective: Rapid maxillary expansion is a common orthodontic procedure to correct maxillary constriction. Assessing the midpalatal suture (MPS) expansion plays a crucial role in treatment planning to determine its effectiveness. The objectives of this preliminary investigation are to demonstrate a proof of concept that the palatal bone underlying the rugae can be clearly imaged by ultrasound (US) and the reconstructed axial view of the US image accurately maps the MPS patency., Methods: An ex-vivo US scanning was conducted on the upper jawbones of two piglet's carcasses before and after the creation of bone defects, which simulated the suture opening. The planar images were processed to enhance bone intensity distribution before being orderly stacked to fuse into a volume. Graph-cut segmentation was applied to delineate the palatal bone to generate a bone volume. The accuracy of the reconstructed bone volume and the suture opening was validated by the micro-computed tomography (µCT) data used as the ground truth and compared with cone beam computed tomography (CBCT) data as the clinical standard. Also included in the comparison is the rugae thickness. Correlation and Bland-Altman plots were used to test the agreement between the two methods: US versus µCT/CBCT., Results: The reconstruction of the US palatal bone volumes was accurate based on surface topography comparison with a mean error of 0.19 mm for pre-defect and 0.15 mm and 0.09 mm for post-defect models of the two samples, respectively when compared with µCT volumes. A strong correlation (R 2 ≥ 0.99) in measuring MPS expansion was found between US and µCT/CBCT with MADs of less than 0.05 mm, 0.11 mm and 0.23 mm for US, µCT and CBCT, respectively., Conclusions: It was possible to axially image the MPS opening and rugae thickness accurately using high-frequency ultrasound., Clinical Significance: This study introduces an ionizing radiation-free, low-cost, and portable technique to accurately image a difficult part of oral cavity anatomy. The advantages of conceivable visualization could promise a successful clinical examination of MPS to support the predictable treatment outcome of maxillary transverse deficiency., Competing Interests: Declaration of competing interest The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article., (Copyright © 2024. Published by Elsevier Ltd.)

Objectives: To compare the impact of three complete denture palatal rugae designs on participants' satisfaction and oral-health-related quality of life., Background: Complete dentures palatal rugea are usually polished to a smooth finish, which can affect the patient's adaptation. Roughening or keeping an opening in the rugae area to replicate the natural sensation of the palatal rugae has been suggested but lacks clinical evidence., Methods: This randomised crossover trial included participants with complete dentures randomly allocated to six sequences. Each sequence alternated between polished, roughened, and open rugae designs. Participants evaluated general and domain-specific satisfaction (eating, taste, speaking, phonetics, and ease of cleaning) on a 100-mm visual analogue scale. They also completed the Oral Health Impact Profile for Edentulous Patients (OHIP-EDENT) and indicated their preferred design at the end. Repeated-measure ANOVA compared satisfaction, and one-way ANOVA with Tukey's test compared OHIP-EDENT scores., Results: Forty-two participants were randomised. Seven dropped out. General satisfaction ratings were comparable for the polished (Mean = 80.2, SD = 19.7) and roughened designs (Mean = 79.5, SD = 20.2). However, the open design had a significantly lower satisfaction rating (Mean = 41.1, SD = 32.2). OHIP-EDENT scores for the open design (Mean = 44.9, SD = 17.5) were significantly worse than those for the polished (Mean = 36.1, SD = 12.7) and roughened designs (Mean = 36.2, SD = 13.3). Two-thirds of the participants chose the polished design. One-third chose the roughened design; of those, 83% were first-time denture wearers., Conclusions: Complete dentures with polished and roughened rugae designs were perceived similarly. Both designs could be recommended based on the patient's preference. However, the open design should be avoided due to negative perception., (© 2024 Gerodontology Association and John Wiley & Sons Ltd.)

Background: To qualitatively and quantitatively review the reliability of palatal rugae as a tool for personal identification following orthodontic treatment. Methods: Cross-sectional retrospective studies assessing the accuracy of matching palatal rugae pattern pre- and post-orthodontic treatment were identified from PubMed and SCOPUS databases. The title and abstract of the articles identified in the search were screened for potential duplicates and relevancy to the topic of interest. The full text of the articles selected in the screening was analyzed using the inclusion and exclusion criteria. Quantitative analysis of the studies representing coherent data in terms of age and treatment choice was performed using RevMan software. Results: Out of 64 screened articles, only 18 articles fulfilled the eligibility criteria and were included in the systematic review. Out of these 18 articles, only 3 studies had data compatible with the quantitative analysis. Significant changes were noted in lateral first rugae in transverse bilateral direction (p = 0.02) and between second and third lateral rugae of the left side in the anteroposterior direction (p = 0.04). Despite the dimensional changes, observers in most studies were able to accurately (>90%) match the palatal rugae pre- and post-orthodontic treatment through visual observation. Conclusion: The accuracy of the visual matching, despite the significant dimensional changes, indicates that morphology could have potentially been the major matching factor. Thus, a combination of dimensional and morphological evaluation of the palatal rugae could potentially increase the accuracy of personal identification.

Background: Palatal rugae have been shown to be associated with racial and geographical variations and are known to be useful in sex identification. The present study aimed to assess palatal rugae patterns in a sample Iranian population and to compare the results with those from other studied populations. Methods: During summer 2017, 130 pre-orthodontic plaster casts from patients aged 17-25 years (65 males and 65 females) were obtained from the Department of Orthodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. The castes were evaluated using the classification given by Thomas and Kotze followed by recording the rugae lengths and shapes. The data were analyzed with the SPSS statistical software package (version 15.0) using the unpaired t test and Chi-square test. P