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21 results on '"Paliwal, Pankaj"'

8. Enhanced in vivo biocompatibility of magnesia-contained bioactive glasses

Author

Sampath Kumar Arepalli, H. S. Tripathi, Sairam Krishnamurthy, S. P. Singh, Partha Pratim Manna, Paliwal Pankaj, Shashikant C. U. Patne, and Ram Pyare

Subjects
010302 applied physics, Materials science, Biocompatibility, medicine.diagnostic_test, Magnesium, chemistry.chemical_element, Complete blood count, 02 engineering and technology, 021001 nanoscience & nanotechnology, medicine.disease, 01 natural sciences, Hemolysis, law.invention, medicine.anatomical_structure, chemistry, In vivo, law, Bioactive glass, 0103 physical sciences, medicine, 0210 nano-technology, Bone regeneration, Blood vessel, Biomedical engineering
Abstract

Human blood compatibility of previously prepared magnesia-doped bioactive glass samples was evaluated by hemolysis assay. The in vivo evaluation was carried out using a rat model in which a hole was made in the rat femur bone by drilling and the bioactive glass samples were implanted in the cavity. Histological examination had demonstrated the better bone regeneration and blood vessel formation as compared to the reference one. The in vivo complete blood count (CBC) was also analyzed at different time periods. The in vivo results of magnesium-containing bioactive glasses had shown improved bioactivity as well as better biocompatibility.

Published
2018

9. Preparation and in vitro investigation on bioactivity of magnesia-contained bioactive glasses

Author

H. S. Tripathi, Ram Pyare, Partha Pratim Manna, Sampath Kumar Arepalli, S. P. Singh, Sairam Krishnamurthy, Shashikant C. U. Patne, and Paliwal Pankaj

Subjects
010302 applied physics, Materials science, Biocompatibility, Magnesium, Scanning electron microscope, Simulated body fluid, chemistry.chemical_element, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, In vitro, Bone resorption, law.invention, chemistry, law, Bioactive glass, 0103 physical sciences, Fourier transform infrared spectroscopy, 0210 nano-technology, Nuclear chemistry
Abstract

Mg plays an important role in the human skeletal system as it stimulates the bone formation and reduces bone resorption. Magnesium has been substituted into SiO2–Na2O–CaO–P2O5 bioactive glass. In the present work, the bioactive glasses were designed to enhance bioactivity. Bioactivity of these glasses was assessed by the immersion of the samples in simulated body fluid (SBF) for different time periods. The formation of hydroxy carbonate apatite (HCA) layer was confirmed by scanning electron microscopy, X-ray diffractometry, and FTIR spectrometry which had shown the HCA layer formation and growth. The formation of HCA layer was found to increase on the surface of the SBF immersed bioactive glasses with increasing magnesia contents in the glass. In vitro cell culture investigations such as viability, proliferation, and cell attachment were studied using human osteosarcoma U2-OS cell lines. The in vitro results of new magnesium containing bioactive glasses had shown improved bioactivity as well as better biocompatibility.

Published
2018

13. Nanoparticle-Induced Controlled Drug Delivery Using Chitosan-Based Hydrogel and Scaffold: Application to Bone Regeneration

Author

Mahanta, Arun Kumar, Senapati, Sudipta, Paliwal, Pankaj, Krishnamurthy, Sairam, Hemalatha, Siva, and Maiti, Pralay

Abstract

The novel chitosan nanohybrid hydrogel and scaffold have been developed with high mechanical strength and tailor the drug release ability for their applications in the biomedical arena. Nanohybrid hydrogels are prepared in dilute acetic acid medium using two different types of two-dimensional-layered nanoparticles. Scaffolds are prepared through lyophilization of hydrogels. Highly porous, open, and 3D interconnected morphologies are observed in the nanohybrid scaffolds, as opposed to the thick wall, smaller pore dimension in pure chitosan. The interaction between the nanoparticles and chitosan chains are elucidated using different spectroscopic techniques, which in turn are responsible for the uniform distribution of the nanoparticle in the chitosan matrix. Nanohybrids are found to be highly mechanically stable in both states (hydrogel and scaffold), as compared to pure chitosan because of the good reinforcing ability of 2D nanoparticles. Sustained drug release has been achieved in nanohybrid in vitro, as compared to the pure chitosan hydrogel/scaffold, mainly due to greater interactions between the components and the better barrier effect of 2D nanoparticles. Cytotoxicity of the nanohybrids is verified using NIH 3T3 mouse embryonic fibroblast cells for their possible use as controlled drug delivery vehicles. Nanohybrids are found to be nontoxic in nature and more biocompatible as compared to pure chitosan, as observed through cell viability and cell imaging studies. Interestingly, cell growth occurs within the pores of the nanohybrid scaffold, vis-à-vis the surface proliferation noticed in the pure chitosan scaffold. Better biocompatibility, hydrophilic nature, and sustained delivery with location specific cell growth make this nanohybrid hydrogel unique for biomedical uses. The bone regeneration rate is found to be significantly higher for the nanohybrid scaffold as compared to blank/pure chitosan without any side effect, suggesting nanohybrid systems are superior biomaterials.

Published
2024
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19. Pharmacokinetics and brain penetration study of chlorogenic acid in rats.

Author

Kumar, Gaurav, Paliwal, Pankaj, Mukherjee, Sumedha, Patnaik, Nishant, Krishnamurthy, Sairam, and Patnaik, Ranjana

Subjects
*PHARMACOKINETICS, *CHLOROGENIC acid, *CEREBROSPINAL fluid, *LABORATORY rats, *NEURODEGENERATION
Abstract

1. The present study is designed to investigate the brain distribution and plasma pharmacokinetics profiles of chlorogenic acid (CGA) after intranasal administration in Charles-Foster rats to evaluate whether the CGA molecules are transported directly via the nose-to-brain path. 2. The CGA is administered intravenously (IV) and intranasally (IN) at the dose of 10 mg/kg. Further, its concentration in the plasma, cerebrospinal fluid (CSF) and the whole brain is analyzed by HPLC-UV method. 3. The study observes that CGA is rapidly absorbed in plasma with tmax of 1 min similar to IV route after IN administration. The peak plasma concentration and AUC0-24 are higher by 3.5 and 4.0 times respectively in IV administration, compared to IN delivery that represents the significant less systemic exposure of CGA in IN route. 4. However, the concentration of CGA in the brain is 4, 6.5, 5.3, 5.2 and 4.5 times higher at 30, 60, 120, 240 and 360 min, respectively in IN administration compared to IV administration. The exposure of CGA in the brain after IN administration (AUCbrain, IN) was significantly greater (4 times) as compared to the exposure of CGA in the brain (AUCbrain, IV) after IV administration reflecting significant brain uptake of CGA through nasal route. Therefore, IN delivery of CGA can be a promising approach for the treatment of stroke and neurodegenerative disorders. [ABSTRACT FROM AUTHOR]

Published
2019
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21. Withania somnifera phytochemicals confer neuroprotection by selective inhibition of nNos: An in silico study to search potent and selective inhibitors for human nNOS.

Author

Kumar, Gaurav, Paliwal, Pankaj, Patnaik, Nishant, and Patnaik, Ranjana

Subjects
*WITHANIA somnifera, *PHYTOCHEMICALS, *NITRIC oxide analysis, *NEUROLOGICAL disorders, *BINDING energy
Abstract

Neuronal nitric oxide synthase (nNOS or NOS1) is an important therapeutic target for the treatment of various neurological diseases. A major challenge faced in the design of nNOS inhibitors emphasizes on potency in humans and selectivity over other NOS isoforms - eNOS and iNOS. The present structural-based in silico study was carried out to search potent and selective inhibitor for human nNOS from a set of 40 Withania somnifera phytochemicals structure. Ten phytochemicals appear as dual-selective inhibitors of nNOS over both iNOS and eNOS. Here we report five potent and selective human nNOS inhibitors, namely, Chlorogenic Acid, Withanolide B, Withacnistin Pelletierine, and Calystegine B2 based on their selectivity, binding energy and nNOS active site residues interaction profile. These phytochemicals have nNOS selectivity higher than 4-methyl-6-(2-(5-(3-(methylamino)propyl)pyridin-3-yl)-ethyl)pyridin-2-amine inhibitor and have potential as an oral neurotherapeutic agent to combat neurological disorders mediated by nNOS activation. [ABSTRACT FROM AUTHOR]

Published
2017
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