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AbstractPogostemon cablin, also known as Patchouli is an essential oil yielding plant with high medicinal properties and having great value for its unique essential oil component. The essential oil is widely used in flavor and fragrance industries. The objective of the present study is to evaluate molecular diversity among mutation treated progeny using RAPD (Randomly amplified polymorphic DNA) and ISSR (Inter Simple Sequence Repeat) markers. The essential oil composition which was analyzed using GC/MS showed patchouli alcohol as major compound, followed by sabinene, (-)β-elemene, α-Guaiene, α-humulene, caryophyllene oxide and viridiflorol in the studied lines. Total 44 RAPD and 34 ISSR primers were used for 41 mutated selected lines, which showed an average polymorphic percentage of 70.1% and 58.14% respectively. Genetic parameters studied by both markers systems were found to be more or less similar. Analysis of molecular variance showed 45% variation among population and 55% within the population by RAPD marker, while ISSR marker showed 36% variation among population and 64% within population. Cluster analysis using both markers grouped the lines into three main clusters and Principal Component Analysis (PCA) plot also supported cluster analysis data. The present study will be helpful in conservation of patchouli germplasm, their characterization and in future breeding programme.

AbstractPogostemon cablinLinn., commonly known as patchouli is an aromatic plant with immense use in aroma and perfumery. The purpose of this study was to examine and compare the chemical composition, antimicrobial activity, and various biological activities of Pogostemon cablinLinn flower and leaf essential oils. The compositional analysis was carried out using GC/MS, while standard biological experiments were used to determine the pharmacological potential of both the essential oils. Patchouli alcohol (27.52%, 44.52%) was found to be the major compound followed by caryophyllene (18.23%, 12.86%) for both flowers and leaves essential oil (EO) respectively. P. cablinflower (PCFEO) and leaf essential oil (PCLEO) exhibited weak to strong antimicrobial activities against selected microbial strains. The mitotic index for flower and leaf EO was 14.38% and 15.20% respectively. PCFEO and PCLEO had chromosomal aberrations of 9.0% and 9.60%, respectively. Both PCFEO and PCLEO had substantial antioxidant activity, with PCLEO exhibiting the highest antioxidant activity (IC50= 1.31 µg/mL). Highest anti-inflammatory activity was exhibited by PCLEO (IC50= 4.49 µg/mL). Similarly, the anti-diabetic ability of PCLEO (IC50= 14.19 µg/mL) was greater than the PCFEO (IC50= 15.60 µg/mL). The PCLEO (IC50= 7.42 µg/mL) and PCFEO (IC50= 12.67 µg/mL) both exhibited better anticholinesterase activity than galanthamine (IC50= 22.16 µg/mL). Better tyrosinase inhibitory activity was shown by PCLEO with IC50= 4.03 µg/mL than PCFEO IC50= 4.44 µg/mL. Apart from their antimicrobial activity, the PCLEO was found to outperform both PCFEO as well as the standards in their respective assays. Thus, the present study affirmed the wide application aspects of patchouli essential oil apart from its aromatic application.

AbstractPatchouli (Pogostemon cablinBenth.) is an industrially valued aromatic and important medicinal plant currently having a huge demand for its essential oil. It is widely used in flavor and fragrance industries as well as in pharmaceuticals. The essential oil is mainly isolated from the dried leaf of patchouli by steam distillation. Patchoulol and α-patchoulene are the major constituents that regulate and control patchouli essential oil quality. The major compounds of this essential oil are patchouli alcohol, α-guaiene, α-bulnesene, β-caryophyllene, α-patchoulene, γ-curcumene and some of the minor compounds are pogostone, limonene, cedrene, viridiflorol, γ-himachalene, etc. The patchouli essential oil is highly valued in perfumery and aromatherapy and is known to have various pharmacological activities such as anti-inflammatory, antiseptic, astringent, diuretic sedative, antimutagenic, antiviral, etc. Realizing the importance and industrial value of the crop, farmers are showing interest in the plantation of patchouli at a commercial scale. In this review, a broad scientific literature were looked through which incorporates Sci-hub, Pub Med, Google Scholar, Pub Chem, NIST chemistry web book and NCBI. The purpose of the review is to summarize the information’s available includes Pogostemon cablinessential oil (EO) extraction and compositions, qualitative and quantitative analysis of the EOs, pharmacological importance, high essential oil yielding variety, adulteration, authentication, patchouli cultivation practices, and industrial demand of patchouli essential oil. An attempt has been made to enlighten the work of various researchers on patchouli carried out to date.

AbstractZingiber zerumbetof the Zingiberaceae family is known as wild edible ginger having various medicinal properties. The present study was aimed at identification of high essential oil yielding genotypes tested through stability parameters at multilocation trials. During the year 2017, a total of 47 genotypes of Z. zerumbetwas collected and planted at experimental farm of CSIR-NEIST, Jorhat, Assam India. A selection trial was conducted for two years viz., 2017 and 2018 for all the collected germplasm which led to the identification of a genotype with high fresh rhizome and essential oil yielding traits. The identified genotype “RRLJ ZB-103” was rich in fresh rhizome yield of 24.86 t/ha and essential oil of 0.75 % (FWB). The selected genotype was subjected to stability analysis along with two check varieties at five multilocation trials for two years. The assessment of stable performance was evaluated using Eberhart and Russell model. Variable environments were found to be present in the study as revealed by the significant variance due to environment and environment (linear). The genotype × environment interaction of the selected genotype also showed significant variance for all the traits revealing the good interaction of the genotype with all the environmental conditions. On comparative assessment, the selected genotype “RRLJ ZB-103” later named “Jor Lab ZB- 103” was found to be highly stable with high adaptability to varying environments for all the traits under study. While both the check varieties revealed low adaptability to varying environments for the higher-yielding traits. The GC/MS analysis of the essential oil of “Jor Lab ZB-103” revealed zerumbone (32.79 %) as the major compound followed by camphene (19.41 %) and eucalyptol (6.80 %). The other compounds were present as minor and trace compounds. The identified high yielding genotype “Jor Lab ZB-103” was found to be a highly stable genotype that could be made available in the public domain for commercial cultivation.

AbstractHomalomena aromaticaSchott. is an important aromatic species used for various purposes like ethnomedicinal, pharmaceutical applications, flavour, and perfume industries. The present study aimed to identify high and stable essential oil-rich accession from collected germplasm of H. aromatica. During the year 2014, a total of 49 germplasm were collected from different parts of NE India. All the collected germplasm were planted in the year 2015 with three replications in Randomized Complete Block Design (RCBD) at the experimental farm of CSIR-NEIST, Jorhat. The same accessions were again planted during 2017 with the same design. All the agronomical and essential oil data were recorded and analyzed. Based on two crops season (2015-16 and 2017-18) of the selection trial, a new essential oil (1.20 %) rich strain (HA-18) with a high dry rhizome yield of 1454.53 kg/ha/crop season was selected. The identified accession was later renamed as Jor Lab SM-2. The stability of the identified germplasm (HA-18) was confirmed by testing in a multilocation trial at four different locations for two years i.e., one crop season (2019-2020) along with two check varieties. The stability analysis (biometric methods) for the economic character like dry rhizome yield, essential oil % and total essential oil yield/ha showed high mean value and were stable in all the four locations with βi=1.05, βi=1.09, and βi=1.14 respectively. The GC/MS profiling of the essential oil isolated from the rhizome of H. aromaticarevealed linalool (66.27 %) as a major compound followed by α-cadinol, tau-muurolol, linalool oxide, neointermedeol, and 3-carene as other compounds. The stable variety Jor Lab SM-2 was found to be more than twice superior compared to the check varieties in terms of essential oil yield. All the studied traits of Jor Lab SM-2 were significantly and distinctly higher than the check varieties. As per our knowledge, no essential oil-rich variant of H. aromaticais reported so far; therefore this is the first report on the identification of a superior and stable line of H. aromatica.

AbstractLantana camaraLinn., a noxious weed found in most places of the world has many potential aspects and has been used as folk medicines to cure several diseases. It is reported to possess antimicrobial, antioxidant, antidiabetic, anti-inflammatory, antiurolithiatic, wound healing, antifertility and cytotoxic activities. The plant leaf essential oil in the present study was obtained by hydrodistillation method and further analyzed by Gas Chromatography/Mass Spectroscopy. Twenty-two compounds were detected owing to 88.97 % of the total essential oil percent. Major compounds identified in the L. camaraleaf essential oil were β-caryophyllene (24.96 %) and δ-selinene (17.46 %), while compounds with intermediate concentration were β-copaene (09.55 %), bicylogermacrene (6.69 %), β-phellandrene (6.49 %) and δ-cadinene (4.95 %). Few of the other minor compounds such as 3-carene (2.74 %), limonene (2.48 %), α-copaene (1.88 %), β-germacrene (1.88 %) and geranyl formate (1.64 %) were also identified in considerable quantities. Among the total identified compounds, 12.79 % of monoterpenes, 0.98 % of alkylbenzene, 1.64 % of fatty alcohol esters and 73.56 % of sesquiterpenes were found in the leaf essential oil of L. camara. The present investigation revealed the chemical profiling of L. camaraleaf essential oil from North East India for the very first time and also highlights their applications as well as a relative analysis of the essential oil composition.

AbstractSyzygium cumini(L.) Skeels (Jambolan) is a widespread summer fruit plant, well-known for various therapeutic uses such as anti-diabetic, antimicrobial, antioxidant properties. The fruit helps to convert starch into energy which helps in regulating blood sugar levels and possesses a low glycemic index. The present study is aimed to estimate the chemical composition of the essential oil of leaves of Syzygium cuminithrough GC/MS. The essential oil was extracted by hydrodistillation using a Clevenger apparatus which was further analyzed through GC/MS. The GC/MS analysis identified twenty-five components with a total area percentage of 95.66 %. The major identified components were τ-cadinol (21.44 %) and tau-muurolol (12.01 %). The compounds of intermediate concentration were globulol (7.98 %), caryophyllene (6.69 %), δ-cadinene (6.56%) and α-pinene (6.32 %). While β-eudesmol (4.42 %), β-pinene (4.32 %), γ-cadinene (3.87 %), camphene (2.98 %), α-terpinenol (2.72 %), camphor (2.23 %), humulene 6,7-epoxide (2.17 %), cubeban-11-ol (1.98 %), α-muurolene (1.81 %), epicubenol (1.43 %), α-copaene (1.26 %), viridiflorene (1.02 %), β-guanine (0.65 %), β-bourbonene (0.23 %), terpinen-4-ol (0.13 %), endo-borneol (0.12 %), levoverbenone (0.12 %) and isobornyl acetate (0.12 %) were the compounds present in minor concentrations. Among the identified compounds there were monoterpene hydrocarbons (13.62 %), bicyclic-monoterpenoid (0.36 %), terpenoid ketones (2.23 %), monocyclic monoterpenoid alkene alcohol (2.85 %), non-oxygenated sesquiterpenes (25.17 %) and oxygenated sesquiterpenes (51.43 %). The present investigation also discusses the applications of various components found in S. cuminileaf essential oil and a comparative view on the essential oil composition of the leaves of the plant was reported earlier.

AbstractCitrus limonL. Burmf (commonly known as lemon) is a small thorn bearing tree which belongs to the family Rutaceae cultivated in the tropical and subtropical regions of the world and mostly cultivated in North Eastern region of India. It bears many medicinal properties and used in the cosmetics industries and pharmaceutical industries. Apart from the plant, extracted peel essential oil of C. limonhas many medicinal properties so it can be used in the treatment of different diseases. Essential oil was extracted using hydrodistillation and analyzed by using Gas chromatography/mass spectroscopy (GC/MS). A total of forty three compounds were identified of which limonene (55.40 %) and neral (10.39 %) were found as major compound followed by trans-verbenol (6.43 %) and decanal (3.25 %). The other compounds present were ethyl cinnamate (2.21 %), ethyl p-methoxycinnamate (2.21 %), cis-α-bergamotene (1.60 %), geraniol (1.48 %), trans-carveol (1.33 %), nonanal (1.19 %), linalool (1.16 %), α-terpineol (1.07 %), p-mentha-2,8-dien-1-ol (0.90 %), estragole (0.73 %), α-fenchene (0.46 %), perillol (0.46 %), β-curcumene (0.45 %), trans-d-limonene oxide (0.43 %), 1-naphthalenamine (0.42 %), camphor (0.34 %), 3-carene (0.34 %), β-santalene (0.27 %), β-sesquiphellandrene (0.25 %), α-pinene (0.17 %), terpinen-4-ol (0.17 %), 3-terpinolenone (0.17 %), zizaene (0.16 %), germacrene -D (0.16 %), α-bisabolol (0.16 %), β-bisabolol (0.14 %), methyl n-hexadecanoate (0.14 %), citronellal (0.12 %), o-cymene (0.12 %), 1-undecanol (0.11 %) and sabinol (0.11 %) were identified as in minor quantities. Limonene was the major chemical compound in the C. limonpeel essential oil which possess many medicinal properties could be used as a pharmaceutical industries.

AbstractAcorus calamusL. is an important aromatic medicinal plant, which has many biological activities. The aim of this study was to evaluate the potential in-vitroantioxidant, anti-microbial, anti-inflammatory and cytotoxic activities of rhizome essential oil of A. calamus. Hydro-distillation process was used to extract the essential oil from dry rhizome using Clevenger apparatus. The chemical compounds identified through GC/MS analysis revealed that β-asarone (82.42 %) as a major compound and calarene (2.41 %), euasarone (1.92 %), 2- methoxy-3-allylphenol (1.91 %) were minor compounds. The antioxidant activity of rhizome essential oil showed good free radical scavenging activity in DPPH assay (IC50=1.68 μg/mL) as compared to standard, ascorbic acid (IC50=1.48 μg/mL). Anti-microbial activity of rhizome essential oil showed the highest zone of inhibition against A. fumigates(16 mm) and lowest against S. cerevisiae(12 mm) at 500 μg/mL concentration among the tested fungal strains whereas, for bacterial strains, the highest zone of inhibition was shown against B. subtilis(12 mm) and lowest against B. cereus(8 mm) at the same concentration. Anti-inflammatory assay showed the IC50value was 172.41 μg/mL whereas in the standard, sodium diclofenac, was 21.0 μg/mL. The toxicity study in A. ceparoots showed that there was negligible effect on the mitotic index (MI=4.20 %) by the rhizome essential oil; as compared to the positive control, EMS (MI=14.79 %) at 1 μL/mL. Based on these findings, it can be concluded that essential oil of A. calamusrhizome could serve as a natural antioxidant and anti-microbial source for commercial use.

AbstractCymbopogonspecies, which belongs to the family Poaceae, is an important genus of medicinal and aromatic plants. The essential oil of the species of genus is rich in various valuable chemical compounds, which are useful for industrial and commercial purposes. In this present study, a total of 425 germplasm of Cymbopogonspecies were collected from different regions of India, and was analyzed for its agronomical, essential oil yield and quality data, for two year 2014-15 and 2015-16.Extraction of essential oil was done using hydro-distillation and the constituents were identified using GC/MS analysis. On the basis of two consecutive year data, a geraniol rich line was identified and the results were confirmed by planting as multi-location trial, along with the standard check during the year 2016-17 and 2017-18. The average essential oil yield of the newly developed variety in the five different locations was 0.54 % and average geranial content of the multi-location trial was 83 %; which is much higher as compared to the check variety that showed essential oil yield of 0.40 % and geraniol content of 46.8 %. This selected germplasm also showed better agronomical traits in comparison to the check variety. This new identified line was named as “Jor Lab L-15” and is suitable for commercial cultivation.

AbstractPsidium guajavaL. (commonly known as guava) is a small tree belonging to the family Myrtaceae. It grows well in the tropical and subtropical regions of the world. It has been used in traditional medicine since a long time. The different parts of the plant like bark, leaves and fruit, is used to cure skin ailments, stomach ache, indigestion, anti-inflammatory, diabetes, hypertension, wounds, pain relief, cough, rheumatic pain and various other ailments. The chemical composition of the whole guava tree renders it the medicinal properties. The chemical composition of the essential oil extracted from the leaves of P. guajava,from North East India, was identified in this study. The leaves yielded 0.51 % (v/w) essential oil. Although there are many reports on the chemical composition of its leaf essential oil; there is no report available in the public domain of the chemical composition of Psidium guajavafrom North East India. Therefore, the leaves of the P. guajavacollected from North East India were hydro-distilled and chemical compounds were identified using GC/MS. A total of 27 compounds were identified in the leaf essential oil. The major compounds were α-terpinyl acetate (23.57 %), trans-caryophyllene (17.65 %), nerolidol (12.16 %), α-cadinol (6.71 %), α-copaene (6.5 %) and minor compounds identified were α-humulene (3.92 %) and (-)-caryphyllene oxide (3.66 %).

AbstractA new high essential oil yielding variety of lemongrass (Cymbopogon flexuosusL.) was developed through mutation breeding technique and registered at ICAR, New Delhi. This genotype was identified through repeated selection in gamma treated mutation populations. After initial screening from M4mutation populations, higher essential oil genotype was identified and planted along with two check varieties in four different locations for two years in NE India and found to be stable for all the related parameters of yield. Average essential oil was recorded as 1.35 % and herbage yield was 29.57 tonnes/ha/year which were significantly higher than the check varieties and total citral content was found as 79.5 % which was followed by other minor compounds viz., linalool, iso-eugenol, limonene, α-pinene, geraniol, camphene, nerol, α-terpineol, tricyclene, α-elemol, geranyl acetate, eugenol acetate, citronellal, bicyclosesquiphellandrene, trans-β-ocimene, trans-β-caryophyllene, humulene epoxide ll, caryophyllene oxide and α-phellandrene. This newly developed novel variety was registered at ICAR, New Delhi vide registration no. INGR-18040 and named as ‘Jor Lab L-14’. This variety propagates vegetatively by means of slips and is stable for commercial cultivation.

AbstractPogostemon cablin(Blanco) Benth, also known as patchouli is an aromatic herb mainly used for high quality perfumery, aromatherapy and fragrances. The study was conducted to identify high yielding mutant genotype of patchouli through gamma radiation. In this experiment 14 different doses of gamma adiation were given to rooted slips of patchouli variety Johor. All 482 M2progenies were produced and their morphological, qualitative and quantitative oil data were recorded. Advanced progenies were developed and finally selected a single plant from each M4progenies on the basis of high essential oil and high herbage yield. These 14 lines were planted in RCBD design along with the check variety with three replications. Significant differences were observed in most of the agronomic characters and oil quality parameters among all mutant progenies. Among all the mutant lines, line no. 9 was found to be superior with plant height, herbage yield per plant, essential oil percent and monopodia length in comparison with the check variety. The multi-location trials revealed that the average fresh herbage yield (960 g/plant), oil content (3.10 %), and patchouli alcohol content (37.94 %) were also found to be significantly higher when compared to the check variety.