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5. Characterization of Lupin Cultivars Based on Phenotypical, Molecular and Metabolomic Analyses

Author

Mavromatis, Athanasios, primary, Nianiou-Obeidat, Irini, additional, Polidoros, Alexios, additional, Parissi, Zoi, additional, Tani, Eleni, additional, Irakli, Maria, additional, Aliferis, Konstantinos A., additional, Zafeiriou, Ioannis, additional, Mylona, Photini V., additional, Sarri, Efi, additional, Papadopoulou, Evgenia-Anna, additional, Tagiakas, Rafail, additional, Kougiteas, Leonidas, additional, Kostoula, Stavroula, additional, and Abraham, Eleni M., additional

Published
2023
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7. GC/EI/MS and 1 H NMR Metabolomics Reveal the Effect of an Olive Tree Endophytic Bacillus sp. Lipopeptide Extract on the Metabolism of Colletotrichum acutatum.

Author

Papadopoulou, Evgenia-Anna, Angelis, Apostolis, Skaltsounis, Alexios-Leandros, and Aliferis, Konstantinos A.

Subjects
OLIVE, COLLETOTRICHUM acutatum, BACILLUS (Bacteria), METABOLOMICS, AMINO acid metabolism, ENDOPHYTES, GAS chromatography/Mass spectrometry (GC-MS), ENDOPHYTIC bacteria
Abstract

The transition to the Green Deal era requires the discovery of alternative sources of bioactivity and an in-depth understanding of their toxicity to target and non-target organisms. Endophytes have recently emerged as a source of bioactivity of high potential for applications in plant protection, used either per se as biological control agents or their metabolites as bioactive compounds. The olive tree endophytic isolate Bacillus sp. PTA13 produces an array of bioactive lipopeptides (LPs), which additionally exhibit reduced phytotoxicity, features that make them candidates for further research focusing on olive tree plant protection. Here, GC/EI/MS and 1H NMR metabolomics were employed to study the toxicity of a Bacillus sp. PTA13 LP extract on the olive tree pathogen Colletotrichum acutatum, which causes the devastating disease olive anthracnose. The discovery of resistant isolates of the pathogen to the applied fungicides makes the research on the development of improved sources of bioactivity of paramount importance. Analyses revealed that the applied extract affects the metabolism of the fungus by interfering with the biosynthesis of various metabolites and its energy production. LPs had a great impact on the aromatic amino acid metabolism, the energy equilibrium of the fungus and its fatty acid content. Additionally, the applied LPs affected the levels of pathogenesis-related metabolites, a finding that supports their potential for further research as plant protection agents. [ABSTRACT FROM AUTHOR]

Published
2023
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9. Discovering the Next-Generation Plant Protection Products: A Proof-of-Concept via the Isolation and Bioactivity Assessment of the Olive Tree Endophyte Bacillus sp. PTA13 Lipopeptides

Author

Papadopoulou, Evgenia-Anna Angelis, Apostolis Antoniadi, Lemonia and Aliferis, Konstantinos A. Skaltsounis, Alexios-Leandros

Abstract

Endophytic microorganisms (EMs) have recently attracted interest for applications in plant protection, mainly due to their bioactive compound-producing capacity. Therefore, we underwent the task of isolating olive tree EMs and investigating their bioactivity against the devastating pathogen Colletotrichum acutatum. Several EMs were isolated; however, the Bacillus sp. PTA13 isolate exhibited the highest toxicity to the phytopathogen. Bacteria of the genus Bacillus exhibit superior bioactive metabolite-producing capacity, with the lipopeptides (LPs) of surfactin, iturin, and fengycin groups being the most studied. A total LP extract and several fractions were obtained, and their bioactivity was assessed against C. acutatum strains. LPs of the major surfactin, iturin, and fengycin groups and the minor gageotetrin and bacilotetrin groups were annotated. The results confirmed the bioactivity of the major LPs, with fengycins being the most fungitoxic. Interestingly, the minor LP fraction exhibited selective toxicity to the fungicide-resistant C. acutatum isolate, an observation that highlights the significance of our approach to comprehensively mine the total LP extract. This work represents a proof of concept of the exploitation of EMs in customized olive tree plant protection and aligns well with strategies that focus on the sustainability and safety of food production via the development of next-generation plant protection products.

Published
2021

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