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Aims: Key herbivory interaction traits such as plant defensive compounds may differ among populations of a single species due to the spatial variation in herbivore feeding guilds and the strength of the interaction. Moreover, the genealogy of population lineages could represent an additional source of variation interacting with the predominant eco-clinal trends. We tested for the existence of genetically based intraspecific variation in chemical defence profiles across the range of the relict tree Prunus lusitanica L. Additionally, we investigated geographical variation in defence inducibility and tested for the existence of a trade-off between qualitative and quantitative defences. Methods: We conducted a greenhouse experiment where 210 plants were grown under a common environment, comprising 10 different populations throughout the distribution range of the species and spanning three separate regions: Iberia, Morocco and Macaronesia. To test for the inducibility of defences, we artificially defoliated plants. Three treatments were established within each population: undamaged, defoliated and sampled after 2 h, and defoliated and sampled after 72 h. The concentration of cyanogenic glycosides (prunasin) and phenolics was determined in leaf samples for all treatments. Important findings: Basal levels of cyanogenics and phenolics significantly differed among populations and regions across the range of P. lusitanica, with this variation having a heritable basis. Cyanogenics (prunasin) were significantly higher in ancient Macaronesian populations, while phenolic concentrations were larger in Iberia. The higher cyanogenic levels found in Macaronesia could be a consequence of the known stronger herbivory pressure in the islands than in Iberia or the likely longer coevolutionary history with herbivores in this region. These findings indicate that the geographical variation of key ecological traits such plant chemical defences can be imprinted by phylogeographical signals, particularly in relict species. Regarding defence inducibility, prunasin increased after simulated herbivory whereas phenolics mostly decreased after defoliation. Variation in defence inducibility across populations and regions was evident, although no consistent patterns related to the variation in herbivore feeding guilds were observed, particularly among regions with and without ungulate browsing pressure. Finally, a trade-off among induced levels of qualitative (prunasin) and quantitative (phenolics) defences was detected in one of the defoliated treatments, likely as a result of a stronger resource limitation in damaged plants. [ABSTRACT FROM AUTHOR]