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339 results on '"Parisi, Silvia"'

2. Experimental evidence and clinical implications of Warburg effect in the skeletal muscle of Fabry disease

Author

Gambardella, Jessica, Fiordelisi, Antonella, Cerasuolo, Federica Andrea, Buonaiuto, Antonietta, Avvisato, Roberta, Viti, Alessandro, Sommella, Eduardo, Merciai, Fabrizio, Salviati, Emanuela, Campiglia, Pietro, D’Argenio, Valeria, Parisi, Silvia, Bianco, Antonio, Spinelli, Letizia, Di Vaia, Eugenio, Cuocolo, Alberto, Pisani, Antonio, Riccio, Eleonora, Di Risi, Teodolinda, Ciccarelli, Michele, Santulli, Gaetano, Sorriento, Daniela, and Iaccarino, Guido

Published
2023
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3. Analysis of CTG patterns in cases with metabolic acidosis at birth with and without neonatal neurological alterations.

Author

Attini, Rossella, Montersino, Benedetta, Versino, Elisabetta, Messina, Alessandro, Mastretta, Emmanuele, Parisi, Silvia, Germano, Chiara, Quattromani, Martina, Casula, Viola, Mappa, Ilenia, Revelli, Alberto, and Masturzo, Bianca

Subjects
ACIDOSIS, CEREBRAL anoxia-ischemia, UMBILICAL arteries, SYMPTOMS, NEWBORN infants
Abstract

Objective: To determine cardiotocographic patterns in newborns with metabolic acidosis, based on clinical signs of neurological alteration (NA) and the need for hypothermic treatment. Methods: All term newborns with metabolic acidosis in a single center from 2016 to 2020 were included in the study. Three segments of intrapartum CTG (cardiotocography) were considered (first 30 min of active labor, 90 to 30 min before birth, and last 30 min before delivery) and a longitudinal analysis of CTG pattern was performed according to the 2015 FIGO classification. Results: Three hundred and twenty-four neonates with metabolic acidosis diagnosed at birth were divided into three groups: the first group included all neonates with any clinical sign of neurological alteration, requiring hypothermia according to the recommendation of the Italian Society of Neonatology (group TNA—Treated neurological Alteration, n = 17), the second encompassed neonates with any clinical sign of neurological alteration not requiring hypothermia (group NTNA—Not Treated neurological Alteration, n = 83), and the third enclosed all neonates without any sign of clinical neurological involvement (group NoNA—No neurological Alteration, n = 224). The most frequent alterations of CTG in TNA group were late decelerations, reduced variability, bradycardia, and tachysystole. Unexpectedly, from the longitudinal analysis of the CTG, 49% of all cases with metabolic acidosis never showed a pathological CTG with normal trace at the beginning of labor followed by normal or suspicious trace in the final part of labor, the same as in TNA and NTNA groups (10 and 39%, respectively). Conclusions: CTG has limited specificity in identifying cases of acidosis at birth, even in babies who will develop NA. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Eumelanin-Based Organic Bioelectronics: Myth or Reality?

Author

Barra, Mario, Bonadies, Irene, Carfagna, Cosimo, Cassinese, Antonio, Cimino, Francesca, Crescenzi, Orlando, Criscuolo, Valeria, Marco, d’Ischia, Maglione, Maria Grazia, Manini, Paola, Migliaccio, Ludovico, Musto, Anna, Napolitano, Alessandra, Navarra, Angelica, Panzella, Lucia, Parisi, Silvia, Pezzella, Alessandro, Prontera, Carmela Tania, and Tassini, Paolo

Published
2016
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18. Outcomes of Pregnancies After Kidney Transplantation: Lessons Learned From CKD. A Comparison of Transplanted, Nontransplanted Chronic Kidney Disease Patients and Low-Risk Pregnancies: A Multicenter Nationwide Analysis

Author

Piccoli, Giorgina Barbara, Cabiddu, Gianfranca, Attini, Rossella, Gerbino, Martina, Todeschini, Paola, Perrino, Maria Luisa, Manzione, Ana Maria, Piredda, Gian Benedetto, Gnappi, Elisa, Caputo, Flavia, Montagnino, Giuseppe, Bellizzi, Vincenzo, Di Loreto, Pierluigi, Martino, Francesca, Montanaro, Domenico, Rossini, Michele, Castellino, Santina, Biolcati, Marilisa, Fassio, Federica, Loi, Valentina, Parisi, Silvia, Versino, Elisabetta, Pani, Antonello, and Todros, Tullia

Published
2017
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19. MicroRNA Roles in Cell Reprogramming Mechanisms

Author

Pascale, Emilia, Caiazza, Carmen, Paladino, Martina, Parisi, Silvia, Passaro, Fabiana, Caiazzo, Massimiliano, Pascale, Emilia, Caiazza, Carmen, Paladino, Martina, Parisi, Silvia, Passaro, Fabiana, and Caiazzo, Massimiliano

Abstract

Cell reprogramming is a groundbreaking technology that, in few decades, generated a new paradigm in biomedical science. To date we can use cell reprogramming to potentially generate every cell type by converting somatic cells and suitably modulating the expression of key transcription factors. This approach can be used to convert skin fibroblasts into pluripotent stem cells as well as into a variety of differentiated and medically relevant cell types, including cardiomyocytes and neural cells. The molecular mechanisms underlying such striking cell phenotypes are still largely unknown, but in the last decade it has been proven that cell reprogramming approaches are significantly influenced by non-coding RNAs. Specifically, this review will focus on the role of microRNAs in the reprogramming processes that lead to the generation of pluripotent stem cells, neurons, and cardiomyocytes. As highlighted here, non-coding RNA-forced expression can be sufficient to support some cell reprogramming processes, and, therefore, we will also discuss how these molecular determinants could be used in the future for biomedical purposes.

Published
2022

20. MicroRNA Roles in Cell Reprogramming Mechanisms

Author

Afd Pharmaceutics, Pharmaceutics, Pascale, Emilia, Caiazza, Carmen, Paladino, Martina, Parisi, Silvia, Passaro, Fabiana, Caiazzo, Massimiliano, Afd Pharmaceutics, Pharmaceutics, Pascale, Emilia, Caiazza, Carmen, Paladino, Martina, Parisi, Silvia, Passaro, Fabiana, and Caiazzo, Massimiliano

Published
2022

23. Additional file 6 of Hmga2 protein loss alters nuclear envelope and 3D chromatin structure

Author

Divisato, Giuseppina, Chiariello, Andrea M., Esposito, Andrea, Zoppoli, Pietro, Zambelli, Federico, Elia, Maria Antonietta, Pesole, Graziano, Incarnato, Danny, Passaro, Fabiana, Piscitelli, Silvia, Oliviero, Salvatore, Nicodemi, Mario, Parisi, Silvia, and Russo, Tommaso

Abstract

Additional file 6: Figure S4. Absence of nuclear alterations in Hmga2 KO undifferentiated cells. Lmnb1 (red) and Emerin (green) immunofluorescence on undifferentiated Hmga2 wt and KO cells showing the absence of nuclear abnormalities. DAPI (blue) was used to counterstain the nuclei. Maximum projection of z-slices (ROI 1024x1024) is shown. Scale bar = 50 μm.

Published
2022
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24. Additional file 7 of Hmga2 protein loss alters nuclear envelope and 3D chromatin structure

Author

Divisato, Giuseppina, Chiariello, Andrea M., Esposito, Andrea, Zoppoli, Pietro, Zambelli, Federico, Elia, Maria Antonietta, Pesole, Graziano, Incarnato, Danny, Passaro, Fabiana, Piscitelli, Silvia, Oliviero, Salvatore, Nicodemi, Mario, Parisi, Silvia, and Russo, Tommaso

Abstract

Additional file 7: Figure S5. Hmga2 wt and KO cells showed a similar localization of several histone H3 modifications upon the induction of EpiLCs. Immunoflurescence experiments on Hmga2 wt and KO cells at day 1 after the induction of EpiLCs. Lmnb1 (red) and H3K4me3, H3K9me2, H3K27me3 and H3K27ac (green) as indicated. Scale bars=50 μm. Quantification graphs obtained by counting >200 nuclei/condition. Error bars represent standard deviation. Statistical significance on three biological replicate experiments was determined using the student’s t-test, ns: not significant).

Published
2022
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25. Additional file 4 of Hmga2 protein loss alters nuclear envelope and 3D chromatin structure

Author

Divisato, Giuseppina, Chiariello, Andrea M., Esposito, Andrea, Zoppoli, Pietro, Zambelli, Federico, Elia, Maria Antonietta, Pesole, Graziano, Incarnato, Danny, Passaro, Fabiana, Piscitelli, Silvia, Oliviero, Salvatore, Nicodemi, Mario, Parisi, Silvia, and Russo, Tommaso

Abstract

Additional file 4: Figure S2. Histone marks at Hmga2 peaks in wt and Hmga2 KD EpiSCs. Chromatin from EpiSCs transfected with control (WT) and Hmga2 targeting siRNAs (KD) was immunoprecipitated with IgG or anti-H3K4me1 or anti-H3K27ac antibodies. Immunoprecipitated DNA was amplified by using oligo pairs annealing to the indicated Hmga2 peaks, which were associated by ChIP-seq with H3K4me1 and/or H3K27ac peaks. A region overlapping the miR-23a gene enhancer was used as a control; Hmga2 peak 10319, not associated with histone modifications, was used as a negative control.

Published
2022
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26. Additional file 8 of Hmga2 protein loss alters nuclear envelope and 3D chromatin structure

Author

Divisato, Giuseppina, Chiariello, Andrea M., Esposito, Andrea, Zoppoli, Pietro, Zambelli, Federico, Elia, Maria Antonietta, Pesole, Graziano, Incarnato, Danny, Passaro, Fabiana, Piscitelli, Silvia, Oliviero, Salvatore, Nicodemi, Mario, Parisi, Silvia, and Russo, Tommaso

Abstract

Additional file 8: Figure S6. Treatment of wt and KO cells with PRC2 inhibitor and its effect on nuclear lamina. (A) Western blotting image showing the level of H3K27me3 histone modifications in Hmga2 wt and KO PSCs treated or not with PRC2 inhibitor at day 0 and at day 1 after the induction of the transition into EpiLCs. The levels of the histone H3 were used as control. (B) Immunoflurescence experiments on Hmga2 wt and KO cells treated or not with PRC2 inhibitor at day 1 after the induction of EpiLCs showing the nuclear lamina phenotype. All pictures are shown as single z-plane, ROI 1024x1024, scale bar=50μm. Quantification graph showing the percentage of cells with nuclear lamina distortion in Hmga2 wt and KO cells treated or not with PRC2 inhibitor (n≤100 nuclei per condition). The count was performed at day 1 of EpiLC transition on three biological replicates. Error bars represent standard deviation. Statistical significance was determined using the student’s t-test (ns: not significant).

Published
2022
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27. Additional file 1 of Hmga2 protein loss alters nuclear envelope and 3D chromatin structure

Author

Divisato, Giuseppina, Chiariello, Andrea M., Esposito, Andrea, Zoppoli, Pietro, Zambelli, Federico, Elia, Maria Antonietta, Pesole, Graziano, Incarnato, Danny, Passaro, Fabiana, Piscitelli, Silvia, Oliviero, Salvatore, Nicodemi, Mario, Parisi, Silvia, and Russo, Tommaso

Abstract

Additional file 1: Figure S1. Hmga2 western blot in wt and KO cells; quantification of western blot of Fig. 1A. (A) western blot analysis of wt and KO cells with Hmga2 ab; Gapdh was used as loading control. (B) The data in the graphs represent the mean ± SD (n=3 biological replicates) of H3K27me3, H3K4me3, H3K9me2, H3K9me3 and H3K27ac against total H3. Student’s t-test, two tailed (ns: not significant, *p

Published
2022
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28. Additional file 5 of Hmga2 protein loss alters nuclear envelope and 3D chromatin structure

Author

Divisato, Giuseppina, Chiariello, Andrea M., Esposito, Andrea, Zoppoli, Pietro, Zambelli, Federico, Elia, Maria Antonietta, Pesole, Graziano, Incarnato, Danny, Passaro, Fabiana, Piscitelli, Silvia, Oliviero, Salvatore, Nicodemi, Mario, Parisi, Silvia, and Russo, Tommaso

Abstract

Additional file 5: Figure S3. Lmnb1 abnormalities occur during the transition from the naïve state to the primed state of pluripotency. (A) Western blotting image showing Lmnb1 expression in Hmga2 wt and KO cells at day 3 after the induction of EpiLCs. Two different clones of PSCs were induced into EpiLCs for each condition. Western blotting quantification revealed no differences in terms of Lmnb1 expression between Hmga2 wild type and knock-out cells. The data in the graphs represent the mean ± SD of the Lmnb1 signal against Gapdh (n=3 biological replicates). ns: not significant (Student’s t-test, two tailed). (B) Lmnb1 immunofluorescence (red) on undifferentiated Hmga2 wt and KO cells showing no structural abnormalities of the nuclei. DAPI (blue) was used to counterstain the nuclei. A single plane of z-stack projection is shown. Scale bar = 50 μm. The data in the graphs represent the mean ± SD of the Lmnb1 signal against Gapdh (n=3 biological replicates). ns: not significant (Student’s t-test, two tailed). (C) q-PCR analysis to evaluate the levels of Hmga2 mRNA in EpiLCs upon transfection of siRNA. Non-silencing siRNA was used as control. The data in the graphs are reported as mean ± SD of relative values (fold changes) obtained normalizing the data of Hmga2 silencing with those of non-silencing control siRNA. **p

Published
2022
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37. Germ Line Mutations in the Thyroid Hormone Receptor Alpha Gene Predispose to Cutaneous Tags and Melanocytic Nevi

Author

Di Cicco, Emery, primary, Moran, Carla, additional, Visser, W. Edward, additional, Nappi, Annarita, additional, Schoenmakers, Erik, additional, Todd, Pamela, additional, Lyons, Greta, additional, Dattani, Mehul, additional, Ambrosio, Raffaele, additional, Parisi, Silvia, additional, Salvatore, Domenico, additional, Chatterjee, Krishna, additional, and Dentice, Monica, additional

Published
2021
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39. Liver organoids: Updates on disease modeling and biomedical applications

Author

Caiazza, Carmen, Parisi, Silvia, Caiazzo, Massimiliano, Caiazza, Carmen, Parisi, Silvia, and Caiazzo, Massimiliano

Abstract

Liver organoids are stem cell-derived 3D structures that are generated by liver differentiation signals in the presence of a supporting extracellular matrix. Liver organoids overcome low complexity grade of bidimensional culture and high costs of in vivo models thus representing a turning point for studying liver disease modeling. Liver organoids can be established from different sources as induced pluripotent stem cells (iPSCs), embryonic stem cells (ESCs), hepatoblasts and tissue-derived cells. This novel in vitro system represents an innovative tool to deeper understand the physiology and pathological mechanisms affecting the liver. In this review, we discuss the current advances in the field focusing on their application in modeling diseases, regenerative medicine and drug discovery.

Published
2021

48. Insights into the pathogenesis of ATP1A1 ‐related CMT disease using patient‐specific iPSCs

Author

Manganelli, Fiore, primary, Parisi, Silvia, additional, Nolano, Maria, additional, Miceli, Francesco, additional, Tozza, Stefano, additional, Pisciotta, Chiara, additional, Iodice, Rosa, additional, Provitera, Vincenzo, additional, Cicatiello, Rita, additional, Zuchner, Stephan, additional, Taglialatela, Maurizio, additional, Russo, Tommaso, additional, and Santoro, Lucio, additional

Published
2019
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