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23 results on '"Park, Yeon-suk"'

22. Sox9 Increases the Proliferation and Colony-forming Activity of Outer Root Sheath Cells Cultured In Vitro.

Author

Shi G, Sohn KC, Kim SY, Ryu EK, Park YS, Lee Y, Seo YJ, Lee JH, and Kim CD

Abstract

Background: β-catenin plays a pivotal role in hair follicle development and hair growth cycle., Objective: The aim of this study was to identify β-catenin-regulated genes in cultured human hair outer root sheath (ORS) cells., Methods: Primary cultured ORS cells were transduced with recombinant adenovirus expressing N-terminal truncated β-catenin (constitutive active form), and β-catenin-regulated genes were identified., Results: Overexpression of the constitutively active form of β-catenin led to induction of Sox9 expression at both mRNA and protein levels. To investigate the potential role of Sox9, we made the recombinant adenovirus expressing green fluorescent protein-tagged Sox9, and then transduced into cultured ORS cells. Interestingly, Sox9 induced the expression of keratin 15, increased the proliferation of ORS cells in vitro, and enhanced colony-forming activity., Conclusion: Our results suggest that Sox9 is a β-catenin-regulated gene in ORS cells, and has potential importance in the regulation of hair follicle homeostasis.

Published
2011
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23. HDAC inhibitors downregulate MRP2 expression in multidrug resistant cancer cells: implication for chemosensitization.

Author

Kim H, Kim SN, Park YS, Kim NH, Han JW, Lee HY, and Kim YK

Subjects
Antineoplastic Agents pharmacology, Cell Line, Tumor, Dose-Response Relationship, Drug, Down-Regulation drug effects, Down-Regulation genetics, Drug Evaluation, Preclinical, Drug Resistance, Multiple drug effects, Drug Resistance, Neoplasm drug effects, Drug Synergism, Gene Expression Regulation, Neoplastic drug effects, Humans, Hydroxamic Acids pharmacology, Multidrug Resistance-Associated Protein 2, Multidrug Resistance-Associated Proteins metabolism, Multidrug Resistance-Associated Proteins physiology, Neoplasms metabolism, Neoplasms pathology, Paclitaxel pharmacokinetics, Paclitaxel pharmacology, Vorinostat, Drug Resistance, Multiple genetics, Drug Resistance, Neoplasm genetics, Histone Deacetylase Inhibitors pharmacology, Multidrug Resistance-Associated Proteins genetics, Neoplasms genetics
Abstract

Although histone deacetylase (HDAC) inhibitors are emerging as a promising class of cancer chemotherapeutic agents, their effects on multidrug resistance (MDR) are poorly understood. In this study, we investigated whether HDAC inhibitors overcome MDR phenotype. HDAC inhibitors suppress the growth of both MDR positive cancer cells KBV20C and its parental cells KB with similar potencies. In parallel, histone acetylation and p21WAF1 expression by the HDAC inhibitors were similarly increased in both cell types, indicating that these HDAC inhibitors are poor substrates of ABC drug transporters and effective in MDR cancer cells. In addition, multidrug resistance protein 2 (MRP2) expression is selectively attenuated by HDAC inhibitors, especially SAHA and TSA, in KBV20C cells, whereas MDR1 and BCRP expressions are not affected. This downregulation of MRP2 contributes to increase in paclitaxel-induced G2/M arrest and apoptosis, which might be due to intracellular accumulation of paclitaxel. Collectively, our data provide a molecular rationale for the application of HDAC inhibitors to overcome MDR in cancer cells.

Published
2011
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