1. Tonsil conventional dendritic cells are not infected by porcine reproductive and respiratory syndrome virus.
- Author
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Puebla-Clark, Lucinda, Parra-Sánchez, Héctor, Reséndiz, Mónica, Valenzuela, Olivia, and Hernández, Jesús
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PORCINE reproductive & respiratory syndrome , *DENDRITIC cells , *TONSILS , *LYMPHOID tissue - Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) infects monocyte-derived DCs, and previous reports have shown that PRRSV does not infect conventional DCs (cDCs) in vitro , but the effects on cDCs from lymphoid tissues are unknown. This study analyzed the response and susceptibility of tonsil DEC205+cDCs from infected pigs. We confirmed the phenotype and lineage of bona fide tonsil cDCs with the mRNA expression of FLT3+ and the phenotype MHCII+CADM1highDEC205+ (DEC205+cDCs). These cells were not infected by PRRSV, whereas CD163+ tonsil cells were infected. The numbers of tonsil cDCs and CD163+ cells were not affected by PRRSV, in contrast to the reduction in alveolar macrophage numbers. DEC205+cDCs exhibited an increase in the expression of IL-12 at 5 days postinfection, suggesting a proinflammatory response by these cells to the virus. In summary, this study confirms that, in vitro and in vivo , cDCs are not susceptible to PRRSV but can respond against it. • We evaluated porcine MHCII+CADM1highDEC205+, bona fide cDCs expressing FLT3+ mRNA. • Tonsil cDCs are not susceptible to PRRSV infection. • Tonsil cDCs increased the IL-12 mRNA expression in response to PRRSV infection. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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