Search

Your search keyword '"Partlow, Benjamin"' showing total 38 results
Start Over Author "Partlow, Benjamin"
38 results on '"Partlow, Benjamin"'

15. 3D Printing of Regenerated Silk Fibroin and Antibody-Containing Microstructures via Multiphoton Lithography

Author

Dickerson, Matthew B., Dennis, Patrick B., Tondiglia, Vincent P., Nadeau, Lloyd J., Singh, Kristi M., Drummy, Lawrence F., Partlow, Benjamin P., Brown, Dean P., Omenetto, Fiorenzo G., Kaplan, David L., and Naik, Rajesh R.

Abstract

Regenerated silk fibroin, a biopolymer derived from silkworm cocoons, is a versatile material that has been widely explored for a number of applications (e.g., drug delivery, tissue repair, biocompatible electronics substrates, and optics) due to its attractive biochemical properties and processability. Here, we report on the free-form printing of silk-based, 3D microstructures through multiphoton lithography. Utilizing multiphoton lithography in conjunction with specific photoinitiator chemistry and postprint cross-linking, a number of microarchitectures were achieved including self-supporting fibroin arches. Further, the straightforward production of high fidelity and biofunctional protein architectures was enabled through the printing of aqueous fibroin/immunoglobulin solutions.

Published
2024
Full Text
View/download PDF

17. Elastic, silk-cardiac extracellular matrix hydrogels exhibit time-dependent stiffening that modulates cardiac fibroblast response

Author

Stoppel, Whitney L., Gao, Albert E., Greaney, Allison M., Partlow, Benjamin P., Bretherton, Ross C., Kaplan, David L., and Black, Lauren D.

Subjects
Male, Tissue Engineering, Tissue Scaffolds, Swine, Myocardium, fungi, technology, industry, and agriculture, Silk, Hydrogels, macromolecular substances, Fibroblasts, Bombyx, Article, Elasticity, Extracellular Matrix, Rats, Sprague-Dawley, Animals, Cells, Cultured, Cell Proliferation
Abstract

Heart failure is the leading cause of death in the United States and rapidly becoming the leading cause of death worldwide. While pharmacological treatments can reduce progression to heart failure following myocardial infarction, there still exists a need for new therapies that promote better healing postinjury for a more functional cardiac repair and methods to understand how the changes to tissue mechanical properties influence cell phenotype and function following injury. To address this need, we have optimized a silk-based hydrogel platform containing cardiac tissue-derived extracellular matrix (cECM). These silk-cECM hydrogels have tunable mechanical properties, as well as rate-controllable hydrogel stiffening over time. In vitro, silk-cECM scaffolds led to enhanced cardiac fibroblast (CF) cell growth and viability with culture time. cECM incorporation improved expression of integrin an focal adhesion proteins, suggesting that CFs were able to interact with the cECM in the hydrogel. Subcutaneous injection of silk hydrogels in rats demonstrated that addition of the cECM led to endogenous cell infiltration and promoted endothelial cell ingrowth after 4 weeks in vivo. This naturally derived silk fibroin platform is applicable to the development of more physiologically relevant constructs that replicate healthy and diseased tissue in vitro and has the potential to be used as an injectable therapeutic for cardiac repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3058-3072, 2016.

Published
2016

18. Injectable, elastic silk-ECM hydrogels for cardiac repair

Author

Kaplan David, Black Lauren, Partlow Benjamin, Stoppel Whitney, Greaney Allison, and Gao Albert

Subjects
Histology, SILK, Materials science, Cardiac repair, Self-healing hydrogels, Biomedical Engineering, Bioengineering, Biotechnology, Biomedical engineering
Published
2016

20. 3D Printing of Regenerated Silk Fibroin and Antibody-Containing Microstructures via Multiphoton Lithography

Author

Dickerson, Matthew B., primary, Dennis, Patrick B., additional, Tondiglia, Vincent P., additional, Nadeau, Lloyd J., additional, Singh, Kristi M., additional, Drummy, Lawrence F., additional, Partlow, Benjamin P., additional, Brown, Dean P., additional, Omenetto, Fiorenzo G., additional, Kaplan, David L., additional, and Naik, Rajesh R., additional

Published
2017
Full Text
View/download PDF

30. Highly Tunable Elastomeric Silk Biomaterials

Author

Partlow, Benjamin P., primary, Hanna, Craig W., additional, Rnjak-Kovacina, Jelena, additional, Moreau, Jodie E., additional, Applegate, Matthew B., additional, Burke, Kelly A., additional, Marelli, Benedetto, additional, Mitropoulos, Alexander N., additional, Omenetto, Fiorenzo G., additional, and Kaplan, David L., additional

Published
2014
Full Text
View/download PDF

31. The use of silk-based devices for fracture fixation

Author

Perrone, Gabriel S., primary, Leisk, Gary G., additional, Lo, Tim J., additional, Moreau, Jodie E., additional, Haas, Dylan S., additional, Papenburg, Bernke J., additional, Golden, Ethan B., additional, Partlow, Benjamin P., additional, Fox, Sharon E., additional, Ibrahim, Ahmed M. S., additional, Lin, Samuel J., additional, and Kaplan, David L., additional

Published
2014
Full Text
View/download PDF

35. Methods and Applications of Multilayer Silk Fibroin Laminates Based on Spatially Controlled Welding in Protein Films.

Author

Brenckle, Mark A., Partlow, Benjamin, Tao, Hu, Applegate, Matthew B., Reeves, Andrew, Paquette, Mark, Marelli, Benedetto, Kaplan, David L., and Omenetto, Fiorenzo G.

Subjects
*SILK fibroin, *TISSUES, *NANOFABRICATION, *MICROFABRICATION, *ELECTRIC transients, *MEDICAL sciences
Abstract

Recent use of biopolymers as interface materials between planar, inorganic electronics and biological tissues has required the adaptation of micro- and nanofabrication techniques for use with these nontraditional materials. In this work, a method which builds on this principle for spatial control of adhesion in multilayer silk fibroin laminates is investigated. This is accomplished through the addition of a spatially patterned amorphous silk adhesive layer in between the films to be adhered, before thermally processing them with heat (120 °C) and pressure (80 Psi) according to established procedures. A one-step method for rapid, high-throughput fabrication is demonstrated, which establishes a strong (1100 kPa) bond between the layers independent of the initial processing conditions of the films. The adhesive layers can be patterned using existing silk fabrication techniques, allowing for the assembly of complex geometries including bilayers and microbubbles. Additionally, the utility of this method is demonstrated for potential applications in drug delivery and transient electronics. This approach provides a versatile method for construction of complex multilayer structures in silk, which with future work may ultimately improve the utility of this material as a bridge between high technology and the biomedical sciences. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

37. Interface Control of Semicrystalline Biopolymer Filmsthrough Thermal Reflow.

Author

Brenckle, Mark A., Partlow, Benjamin, Tao, Hu, Kaplan, David L., and Omenetto, Fiorenzo G.

Subjects
*BIOPOLYMERS, *CRYSTALLINE polymers, *FABRICATION (Manufacturing), *ELECTRONICS, *MEDICINE, *SILK fibroin
Abstract

The recent increase in the developmentof polymer-based technologicaldevices has necessitated a surge in associated fabrication strategies.An adequate understanding of the interfacial properties in such systemsis required to meet the challenges inherent to these applications,ranging from electronics to biomedicine. In this work, we utilizethermal reflow to investigate the interfacial properties of multilayersilk fibroin film constructs. We demonstrate that reflow can be utilizedto control the water content, glass transition, and β sheetcrystallinity of such constructs, leading to control of the mechanicalproperties at the interface. Such analysis may lend insight into theinterfacial properties of similar semicrystalline biopolymers, increasingthe number of fabrication options for the development of devices atthe biological–technological nexus. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results