Search

Your search keyword '"Pascale, Maria Rosaria"' showing total 169 results
Start Over Author "Pascale, Maria Rosaria"
169 results on '"Pascale, Maria Rosaria"'

4. Applicability of 2022 classifications of acute myeloid leukemia in the real-world setting

Author

Attardi, Enrico, Savi, Arianna, Borsellino, Beatrice, Piciocchi, Alfonso, Cipriani, Marta, Ottone, Tiziana, Fabiani, Emiliano, Divona, Mariadomenica, Travaglini, Serena, Pascale, Maria Rosaria, Awada, Hussein, Durmaz, Arda, Visconte, Valeria, Della Porta, Matteo Giovanni, Venditti, Adriano, Maciejewski, Jaroslaw P., Gurnari, Carmelo, and Voso, Maria Teresa

Published
2023
Full Text
View/download PDF

5. Surfaces environmental monitoring of SARS-CoV-2: Loop mediated isothermal amplification (LAMP) and droplet digital PCR (ddPCR) in comparison with standard Reverse-Transcription quantitative polymerase chain reaction (RT-qPCR) techniques.

Author

Spiteri, Simona, Salamon, Irene, Girolamini, Luna, Pascale, Maria Rosaria, Marino, Federica, Derelitto, Carlo, Caligaris, Laura, Paghera, Simone, Ferracin, Manuela, and Cristino, Sandra

Subjects
LOOP-mediated isothermal amplification, COVID-19, NUCLEIC acid isolation methods, LONG-term care facilities, POLYMERASE chain reaction, SARS-CoV-2
Abstract

The persistence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) on substrates, and the impact of fomites on Coronavirus Disease 19 (COVID-19) transmission, is until now, widely discussed. Consequently, further investigations are required for a correct risk assessment in high-risk facilities such as hospitals, healthcare facilities (HCFs), and long-term care facilities (LTCFs). Therefore, appropriate surveillance and disinfection programs represent the best approach to guarantee the safety of these communities. This study proposes an environmental SARS-CoV-2 surfaces routine monitoring approach in HCF and communities' settings, to provide rapid and effective evaluation of surface hygienic conditions and the effectiveness of applied sanitization measures. Surfaces samples (n = 118) were collected using the SRK® kit (Copan Italia) from 2020 to 2023. Three molecular techniques were compared: Reverse Transcription Loop mediated isothermal AMPlification (RT-LAMP, Enbiotech), Reverse-Transcription quantitative polymerase chain reaction (RT-qPCR) (RT-qPCR, Seegene) and droplet digital PCR (ddPCR, Bio-Rad). For ddPCR, two RNA extraction methods were compared: TRIzol LS (Invitrogen) versus QIAmp Viral Mini kit (QIAGEN), showing how the latter is more suitable for surfaces. Regarding the quantitative ddPCR results, the ROC analysis allowed to reduce the manufacturer cut-off for droplets number (from 3 to 1) for the positive samples. Moreover, a new cut-off for the viral RNA copies' number/μL for each target (N1 and N2) on environmental monitoring was fixed at 2,82. The results obtained using the QIAmp kit, suggested that the N2 target is more stable in the environment and could be most suitable for the virus environmental detection. The percentage of positive samples was similar among the techniques (26% for RT-LAMP, 36% for ddPCR and 23% for RT-qPCR). Using RT-qPCR as reference method, a sensitivity (SE) of 30% for RT-LAMP and 41% for ddPCR was observed. By contrast, specificity (SP) was higher for RT-LAMP (75%) respect to ddPCR (66%). Comparing the faster RT-LAMP with the sensitive ddPCR the 26% and 74% of SE and SP for RT-LAMP, were reported. The low sensitivity for RT-LAMP and ddPCR could be explained with the use of clinical rather than environmental kits, other than the changing in the virus prevalence during the sampling campaign. Although the RT-LAMP requires improvements in term of SE and SP, this research presents an innovative environmental monitoring and prevention method for SARS-CoV-2, that could be extended to other pathogens that are under environmental surveillance. [ABSTRACT FROM AUTHOR]

Published
2025
Full Text
View/download PDF

7. Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2.

Author

Spiteri, Simona, Marino, Federica, Girolamini, Luna, Pascale, Maria Rosaria, Derelitto, Carlo, Caligaris, Laura, Paghera, Simone, and Cristino, Sandra

Subjects
REVERSE transcriptase polymerase chain reaction, PUBLIC health surveillance, COVID-19 pandemic, PROCESS control systems, ENVIRONMENTAL monitoring, SANITATION, GENE amplification
Abstract

The rapid and accurate detection of SARS-CoV-2 in environmental settings is crucial for effective public health management during the COVID-19 pandemic. This study compares the performance of the Reverse Transcription quantitative polymerase chain reaction (RT-qPCR) and the Reverse Transcription loop-mediated isothermal amplification (RT-LAMP) for SARS-CoV-2 detection from 100 surface samples collected in healthcare environments. The reference method, RT-qPCR, identified a percentage of 25% of positive samples, while RT-LAMP detected a percentage of 27% of positive surfaces. Our findings reveal a sensitivity of 32% and specificity of 75% for RT-LAMP, with a positive predictive value of 30% and a negative predictive value of 77%. The overall accuracy and concordance with RT-qPCR was 64% for both methods. Despite its lower sensitivity compared to RT-qPCR, RT-LAMP had an advantage due to its rapid screening and environmental surveillance, which is particularly useful for confirming negative results. These results underscore the potential of RT-LAMP not only as a valuable method in the environmental monitoring of SARS-CoV-2 but also as a system to control the sanitation process in ordinary and emergency conditions, providing further optimization and validation for its reliability in routine surveillance and outbreak response efforts. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

10. New Frontiers in Water Distribution System Management and Monitoring: First Development of a Water Safety Plan Based on Heritage Building Information Modeling (HBIM) in Neptune Fountain, Bologna, Italy.

Author

Pascale, Maria Rosaria, Roggio, Domenico Simone, Barbieri, Ester, Marino, Federica, Derelitto, Carlo, Girolamini, Luna, Bragalli, Cristiana, Bitelli, Gabriele, and Cristino, Sandra

Subjects
WATER distribution, WATER quality monitoring, BUILDING information modeling, HETEROTROPHIC bacteria, HYDRAULIC measurements, COLIFORMS
Abstract

The World Health Organization (WHO) recommends the introduction of a water safety plan (WSP) approach on drinking water, in all types of settings. This study represents the first WSP developed on the Neptune Fountain, in Bologna (Italy), based on an interdisciplinary approach, integrating hydraulic and microbiological features, in a Building Information Modeling (BIM). The aim was to develop a dynamic and digital platform to update and share the maintenance program, promoting collaboration among microbiologists, engineers, and municipal staff. Water samples were collected along fountain water distribution systems (WDS) from 2016 to 2021 to monitor water quality through the heterotrophic bacteria at 22 °C and 37 °C, as well as to conduct an Enterococci, Coliform bacteria, Escherichia coli, Pseudomonas aeruginosa, Clostridium perfringens, and Staphylococcus aureus assessment. Simultaneously, hydraulic measures were performed, and advanced geomatics techniques were used to detect the WDS structural components, with a focus on the water treatment system (WTS). The WTS consisted of 10 modules corresponding to specific treatments: descaling, carbon–sand filtration, reverse osmosis, and ultraviolet disinfection. Fecal indicators, heterotrophic bacteria, and P. aeruginosa exceeded the reference limits in most of the modules. Several disinfections and washing treatments, other than changing the maintenance procedure scheduling, were performed, improving the WTS and controlling the contamination. The developed microbiological results, hydraulic measurements, and maintenance procedures were integrated in the BIM model to optimize the data storage, updating procedures and the real-time data sharing. This approach improved the fountain management, operation, and material conservation, ultimately preserving the health of daily visitors. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

12. Diagnostic capabilities, clinical features, and longitudinal UBA1 clonal dynamics of a nationwide VEXAS cohort.

Author

Gurnari, Carmelo, Pascale, Maria Rosaria, Vitale, Antonio, Diral, Elisa, Tomelleri, Alessandro, Galossi, Elisa, Falconi, Giulia, Bruno, Alessandro, Crisafulli, Francesca, Frassi, Micol, Cattaneo, Chiara, Bertoli, Diego, Bernardi, Massimo, Condorelli, Annalisa, Morsia, Erika, Poloni, Antonella, Crisà, Elena, Caravelli, Daniela, Triggianese, Paola, and Brussino, Luisa

Published
2024
Full Text
View/download PDF

18. Body Composition in Patients with Follicular Lymphoma: Asso-Ciations between Changes in Radiomic Parameters in Patients Treated with R-CHOP-like and R-B Regimens: LyRa 01F

Author

Esposito, Fabiana, primary, Pascale, Maria Rosaria, additional, Tesei, Cristiano, additional, Gigliotti, Paola Elda, additional, Luciano, Alessandra, additional, Angeloni, Cecilia, additional, Marinoni, Massimiliano, additional, Meconi, Federico, additional, Secchi, Roberto, additional, Patanè, Alberto, additional, Postorino, Massimiliano, additional, Cantonetti, Maria, additional, and Manenti, Guglielmo, additional

Published
2023
Full Text
View/download PDF

21. Predictors of Early Thrombotic Events in Adult Patients with Acute Myeloid Leukemia: A Real-World Experience

Author

Paterno, Giovangiacinto, primary, Palmieri, Raffaele, additional, Forte, Vittorio, additional, Del Prete, Valentina, additional, Gurnari, Carmelo, additional, Guarnera, Luca, additional, Mallegni, Flavia, additional, Pascale, Maria Rosaria, additional, Buzzatti, Elisa, additional, Mezzanotte, Valeria, additional, Cerroni, Ilaria, additional, Savi, Arianna, additional, Buccisano, Francesco, additional, Maurillo, Luca, additional, Venditti, Adriano, additional, and Del Principe, Maria Ilaria, additional

Published
2022
Full Text
View/download PDF

23. Case report: A Saprochaete clavata (Magnusiomyces clavatus) severe infection effectively treated with granulocyte transfusion in a young patient with myeloid sarcoma

Author

Pasqualone, Gianmario, primary, Buzzatti, Elisa, additional, Palmieri, Raffaele, additional, Savi, Arianna, additional, Pascale, Maria Rosaria, additional, Borsellino, Beatrice, additional, Guarnera, Luca, additional, Buccisano, Francesco, additional, Voso, Maria Teresa, additional, Maurillo, Luca, additional, Sconocchia, Giuseppe, additional, Venditti, Adriano, additional, and Del Principe, Maria Ilaria, additional

Published
2022
Full Text
View/download PDF

24. Can Polycythemia Vera evolve from Acute Myeloid Leukemia? A Case Report Showing a Simultaneous Minor JAK2 V617F Mutated Clone,

Author

Borsellino, Beatrice, primary, Savi, Arianna, additional, Pascale, Maria Rosaria, additional, Meddi, Elisa, additional, Cristiano, Antonio, additional, OTTONE, TIZIANA, additional, Rapanotti, Maria Cristina, additional, Divona, Mariadomenica, additional, TRAVAGLINI, SERENA, additional, Attardi, Enrico, additional, Buzzatti, Elisa, additional, Buccisano, Francesco, additional, and Voso, Maria Teresa, additional

Published
2022
Full Text
View/download PDF

26. Case Report: First Report of Fatal Legionella pneumophila and Klebsiella pneumoniae Coinfection in a Kidney Transplant Recipient

Author

Scaturro, Maria, primary, Girolamini, Luna, additional, Pascale, Maria Rosaria, additional, Mazzotta, Marta, additional, Marino, Federica, additional, Errico, Giulia, additional, Monaco, Monica, additional, Girolamo, Antonietta, additional, Rota, Maria Cristina, additional, Ricci, Maria Luisa, additional, and Cristino, Sandra, additional

Published
2022
Full Text
View/download PDF

30. In BCR-ABL1 Positive B-Cell Acute Lymphoblastic Leukemia, Steroid Therapy Induces Hypofibrinogenemia

Author

Buzzatti, Elisa, primary, Forghieri, Fabio, additional, Paterno, Giovangiacinto, additional, Marchesi, Francesco, additional, Sarlo, Chiara, additional, Giglio, Fabio, additional, Fracchiolla, Nicola, additional, Sciumè, Mariarita, additional, Palmieri, Raffaele, additional, Esposito, Fabiana, additional, Guarnera, Luca, additional, Mercante, Lisa, additional, Pascale, Maria Rosaria, additional, Mallegni, Flavia, additional, Savi, Arianna, additional, Forte, Vittorio, additional, Maurillo, Luca, additional, Buccisano, Francesco, additional, Venditti, Adriano, additional, and Del Principe, Maria Ilaria, additional

Published
2022
Full Text
View/download PDF

31. Occult central nervous system involvement guides therapeutic choices in blastic plasmacytoid dendritic cell neoplasms

Author

Buzzatti, Elisa, primary, Paterno, Giovangiacinto, additional, Palmieri, Raffaele, additional, Esposito, Fabiana, additional, Pascale, Maria Rosaria, additional, Mallegni, Flavia, additional, Guarnera, Luca, additional, Pasqualone, Gianmario, additional, Irno Consalvo, Maria Antonietta, additional, Fraboni, Daniela, additional, Moretti, Federico, additional, Savi, Arianna, additional, Borsellino, Beatrice, additional, Maurillo, Luca, additional, Buccisano, Francesco, additional, Sconocchia, Giuseppe, additional, Venditti, Adriano, additional, and Del Principe, Maria Ilaria, additional

Published
2022
Full Text
View/download PDF

42. Evaluation of MALDI–TOF Mass Spectrometry in Diagnostic and Environmental Surveillance of Legionella Species: A Comparison With Culture and Mip-Gene Sequencing Technique

Author

Pascale, Maria Rosaria, primary, Mazzotta, Marta, additional, Salaris, Silvano, additional, Girolamini, Luna, additional, Grottola, Antonella, additional, Simone, Maria Luisa, additional, Cordovana, Miriam, additional, Bisognin, Francesco, additional, Dal Monte, Paola, additional, Bucci Sabattini, Maria Antonietta, additional, Viggiani, Mariagabriella, additional, and Cristino, Sandra, additional

Published
2020
Full Text
View/download PDF

47. Can Polycythemia Vera Evolve from Acute Myeloid Leukemia? Report of a Case Showing a Simultaneous Minor JAK2 V617F Mutated Clone.

Author

Borsellino, Beatrice, Savi, Arianna, Pascale, Maria Rosaria, Meddi, Elisa, Del Principe, Maria Ilaria, Cristiano, Antonio, Ottone, Tiziana, Rapanotti, Maria Cristina, Divona, Mariadomenica, Travaglini, Serena, Attardi, Enrico, Palmieri, Raffaele, Buzzatti, Elisa, Buccisano, Francesco, and Voso, Maria Teresa

Subjects
POLYCYTHEMIA vera, ACUTE myeloid leukemia, MOLECULAR cloning, MYELOPROLIFERATIVE neoplasms, BONE marrow
Abstract

The evolution of myeloproliferative neoplasms (MPN) to acute myeloid leukemia (AML) occurs in 2-10% of patients, depending on the MPN subtype, treatment, and follow-up length. The reverse-path from AML to MPN has been rarely reported. We herein present a 75 years old woman with AML, in whom a JAK2-V617F positive polycythemia vera (PV) emerged during follow-up, 19 months from the end of consolidation treatment. JAK2-V617F mutation screening retrospectively performed by Next Generation Sequencing (NGS) and JAK2 MutaScreen was negative on the bone marrow sample collected at AML diagnosis. However, using digital droplet PCR (ddPCR), we detected a minor JAK2 V617F mutated clone at AML onset. In addition, a TET2 R550 mutated clone persisted at stable levels throughout the disease course. This case shows that a very small MPN clone masked at AML diagnosis may expand after treatment end and be erroneously interpreted as MPN evolving from AML. Very sensitive techniques such as ddPCR may help to unravel the true disease history in these cases. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

49. Dall''Apollo medice' delle vestali all''Ars fructosior' di Plinio. Considrazioni sociali e giuridiche

Author

DE PASCALE, MARIA ROSARIA and DE PASCALE, MARIA ROSARIA

Abstract

Nelle fonti viene utilizzato il termine medicus per indicare indistintamente più figure (medici, paramedici, praticoni, ciarlatani e persino impostori). Il riconoscimento della ‘qualità’ di medicus e non di impostor si fonda, verosimilmente, sulla base della fama (pubblica opinione). La considerazione pubblica dei medici a Roma risente, però, notevolmente del fatto che per la loro attività prendano un compenso, per cui diventano oggetto di disprezzo da parte dei più conservatori, che si rifanno agli antichi costumi che non ammettono che la vita e a salute possano dipendere da un salario. Un vero medico non ‘lavora’ bensì pone il suo sapere al servizio dell’uomo: esercitare la professione per un medico è un onore, come per un avvocato, e farsi pagare è un disonore. Dal I secolo d.C., la medicina diviene senza dubbio un’attività professionale retribuita non spregevole (come, invece, ancora i lavori manuali) in virtù del suo livello intellettuale e della sua considerazione ed utilità sociale.

Published
2014

50. 'Radici antiche della previdenza funeraria: sui collegia militari romani'

Author

DE PASCALE, MARIA ROSARIA and DE PASCALE, MARIA ROSARIA

Subjects
casse legionarie, collegia militari
Abstract

La moderna previdenza funeraria affonda radici nel diritto romano di età imperiale per lo specifico ambito professionale militare attraverso la costituzione di casse legionarie e collegia. Le casse legionarie, su base obbligatoria, coprivano le esigenze dei semplici soldati di truppa e fornivano loro allo stesso tempo servizi che oggi definiremmo di tesoreria, di raccolta del risparmio e di erogazione del credito, elementi tipici della struttura bancaria moderna. I collegia, destinati ai soli graduati, che non fruivano dalle casse legionarie e che si aggregavano su base volontaria, raccoglievano e amministravano i fondi dei soci e ne coprivano i rischi derivanti dalla cessazione prematura del servizio o dalla morte improvvisa.

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results