Search

Your search keyword '"Patarino T"' showing total 6 results
Start Over Author "Patarino T"
6 results on '"Patarino T"'

3. Impaired function of CD4CD25 T regulatory lymphocytes characterizes the self-limited hepatitis A virus infection

Author

Costanza Sbreglia, Simona Altamura, Giuseppe Morelli, Alessandro Perrella, Stella Grattacaso, Luigi Racioppi, Luigi Atripaldi, Tommaso Patarino, Laura Vitiello, Oreste Perrella, Pasquale Bellopede, Perrella, A., Vitiello, Laura, Atripaldi, L., Sbreglia, C., Grattacaso, S., Bellopede, P., Patarino, T., Morelli, G., Altamura, S., Racioppi, Luigi, and Perrella, O.

Subjects
Adult, Male, CD3 Complex, CD3, Lymphocyte Activation, T-Lymphocytes, Regulatory, DENDRITIC CELLS, DISEASE, Flow cytometry, INFLAMMATION, medicine, Humans, IMMUNE-RESPONSE, IL-2 receptor, Seroconversion, Cells, Cultured, SUPPRESSION, Hepatitis, Hepatology, biology, medicine.diagnostic_test, business.industry, Interleukin-2 Receptor alpha Subunit, Gastroenterology, Case-control study, T helper cell, Hepatitis A, medicine.disease, Hepatitis a virus, medicine.anatomical_structure, Case-Control Studies, CD4 Antigens, AUTOIMMUNE HEPATITIS, Immunology, biology.protein, Female, business, Hepatitis A Virus, Human
Abstract

Background and Aim: Hepatitis A virus (HAV) causes a transient illness leaving permanent protection against reinfection. Few data are available on the regulatory mechanisms involved in the CD4+ T helper activation. We aimed to investigate the frequency and function of CD3+/CD4+/CD25+ T cells with regulatory function (Tregs) during acute HAV infection. Methods: We enrolled 35 consecutive patients and 15 healthy donors, enumerated Tregs by flow cytometry assay and evaluated, after immunomagnetical sorting with magnetic beads, their ability to inhibit the proliferation of CD4+/CD25– T lymphocytes at different ratios (1:1, 1:10, 1:20). Results: All patients had the usual course of infection. Our immunological analysis showed Tregs frequency in these patients (6.5% [range, 5–8.8%]; 36 [range, 10–87] cells) did not have any statistical difference compared with healthy donors (6% [range, 5–8%]; 48 (range, 23–71) cells), while their ability to suppress CD4+/CD25– was drastically reduced at different ratios (Mann–Whitney U-test; ratio 1:1, 93% vs 72%, z = −3.34, P

Published
2008
Full Text
View/download PDF

4. Elevated CD4+/CD25+ T cell frequency and function during acute hepatitis C presage chronic evolution

Author

Luigi Racioppi, S Altamura, C. Alone, Luigi Atripaldi, Alessandro Perrella, Pio Conti, Costanza Sbreglia, R Vela, Tommaso Patarino, Pasquale Bellopede, Laura Vitiello, Oreste Perrella, Giuseppe Morelli, Perrella, A, Vitiello, L, Atripaldi, L, Conti, P, Sbreglia, C, Altamura, S, Patarino, T, Vela, R, Morelli, G, Bellopede, P, Alone, C, Racioppi, Luigi, and Perrella, O.

Subjects
Adult, CD4-Positive T-Lymphocytes, Male, Interleukin 2, CD3, T cell, Hepatitis C virus, Biology, liver, medicine.disease_cause, T-Lymphocyte Subsets, medicine, Humans, Letters, IL-2 receptor, Receptor, Gastroenterology, Receptors, Interleukin-2, Hepatitis C, Hepatitis C, Chronic, Middle Aged, Prognosis, medicine.disease, Virology, medicine.anatomical_structure, inflammation, Acute Disease, HCV, Immunology, Disease Progression, biology.protein, Female, Alpha chain, T regulatory, medicine.drug
Abstract

Hepatitis C virus (HCV) determines an acute hepatitis evolving to persistent infection in 50–80% of patients.1 Different mechanisms have been proposed to explain disease evolution, including viral escape, failure of the T helper immune network, and host genetic factors.2 Among CD4+ T cell subsets, lymphocytes expressing constitutively CD25 (interleukin 2 receptor alpha chain), namely T regulatory cells (Treg), appear to play a critical role in controlling chronic evolution of HCV mediated liver diseases.3 Here we investigate the frequency and functional activity of CD3+/CD4+/CD25+ Tregs in acute HCV infection in relation to its evolution over time. The study was approved by the local ethics committee and included 16 consecutive patients with acute hepatitis C (AHC), 15 consecutive patients with acute hepatitis A (AHA), and …

Published
2006
Full Text
View/download PDF

5. Flow cytometry assay of myeloid dendritic cells (mDCs) in peripheral blood during acute hepatitis C: possible pathogenetic mechanisms.

Author

Perrella A, Atripaldi L, Bellopede P, Patarino T, Sbreglia C, Tarantino G, Sorrentino P, Conca P, Ruggiero L, and Perrella O

Subjects
Acute Disease, Adult, Antiviral Agents therapeutic use, CD11 Antigens analysis, Case-Control Studies, Cell Count, Disease Progression, Drug Combinations, Female, Hepatitis A blood, Hepatitis A drug therapy, Hepatitis A etiology, Hepatitis A immunology, Hepatitis C drug therapy, Hepatitis C immunology, Humans, Interferons therapeutic use, Lipopolysaccharide Receptors analysis, Male, Middle Aged, Ribavirin therapeutic use, Dendritic Cells immunology, Flow Cytometry, Hepatitis C blood, Hepatitis C etiology, Myeloid Cells immunology
Abstract

Aim: To asses the expression of myeloid dendritic cells (CD11c+) subset during acute HCV hepatitis and its possible involvement in natural history of the infection., Methods: We enrolled 11 patients with acute hepatitis C (AHC) (Group A), 10 patients with acute hepatitis A (AHA) (as infective control-Group B) and 10 healthy donors (group C) in this study. All patients underwent selective flow cytometry gating strategies to assess the peripheral number of the myeloid dendritic cells (mDCs) to understand the possible role and differences during acute hepatitis., Results: Eight of 11 patients with acute HCV hepatitis did not show any increase of mDCs compared to healthy individuals, while a significant decrease of mDCs was found in absolute cell count (z = -2.37; P<0.05) and percentage (z = -2.30; P<0.05) as compared with AHA. On the contrary, The remaining three patients of the group A had a higher mDCs number and percentage as occur in group B. Interestingly, after six months, those patients did not show any increase of mDCs subset were chronically infected. while the three subjects with an increase of peripheral mDCs, as in HAV acute infection, resolved the illness., Conclusion: The lack of increase of mDCs during acute hepatitis C might be an important factor involved in chronicization of the infection.

Published
2006
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results