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466 results on '"Pathak, Devendra"'

21. Conflict Between Artificial Intelligence and Traditional Designers: How Artificial Intelligence Transforms the Design Process

Author

Gupta, Keshav Dev, Pandey, Rishi, Pathak, Devendra Nath, Ranjan, Rakesh, Gupta, Vijay, Kacprzyk, Janusz, Series Editor, Gomide, Fernando, Advisory Editor, Kaynak, Okyay, Advisory Editor, Liu, Derong, Advisory Editor, Pedrycz, Witold, Advisory Editor, Polycarpou, Marios M., Advisory Editor, Rudas, Imre J., Advisory Editor, Wang, Jun, Advisory Editor, Rathore, Vijay Singh, editor, Piuri, Vincenzo, editor, Babo, Rosalina, editor, and Tiwari, Vivek, editor

Published
2024
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26. Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes.

Author

Carlo, Ashritha Q., Pathak, Devendra, Choudhary, Ratan K., Singh, Opinder, and Bansal, Neelam

Subjects
*WATER buffalo, *POLYMERASE chain reaction, *BASAL lamina, *GENE expression, *CELLULAR control mechanisms
Abstract

This study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohistochemical, and ultrastructural analyses. Specifically, we examined changes in the expression of two potential SSC markers, OCT4 and PGP9.5, using immunohistochemistry. Additionally, we conducted a real-time quantitative polymerase chain reaction (RT-qPCR) to assess the relative gene expression of OCT4 and PGP9.5. The relative expression of the OCT4 gene was down-regulated in the adult testes compared to its expression during prepubertal and neonatal life. The relative expression of the PGP9.5 gene was up-regulated in the neonatal testes and down-regulated in the prepubertal and adult testes. The spermatogonia were round, oval-to-ellipsoidal cells lying over the basement membrane (BM) with a round-to-oval nucleus. Based on the immunoexpression of the putative SSC markers, OCT4 and PGP9.5, we concluded that the proportion of stem cells was highest during the neonatal stage, followed by the prepubertal and prenatal stages. This finding sheds light on the dynamics of spermatogonial stem cells in buffalo testes at different developmental stages, providing valuable insights into these cells' regulation and potential applications. [ABSTRACT FROM AUTHOR]

Published
2024
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42. Exploring the Nexus of Steroidal Hormone Receptor, Uterine VEGF Expression and NADPH‐d Interaction in Buffalo Uterus During Oestrous Cycle With Seasonal Variation.

Author

Dehury, Sagarika, Uppal, Varinder, Pathak, Devendra, and Gupta, Anuradha

Subjects
SEXUAL cycle, VASCULAR endothelial growth factors, ESTRUS, SUMMER, HORMONE receptors
Abstract

The reproductive efficiency in buffalo is highly influenced by seasonal variability. Angiogenesis in the reproductive cycle is important for optimal physiological functioning of uterus. Estrogen receptor‐α (ERα), vascular endothelial growth factor (VEGF) and reduced nicotinamide adenine dinucleotide phosphatase diaphorase (NADPH‐d) are vital indicators for the uterine angiogenic process. This study was conducted to see the effect of season on the expression of different uterine angiogenic factors. Season wise (winter and summer) and phase wise (follicular and luteal), immune staining intensity of buffalo uterus was measured by calculating the optical density value (OD) for ERα and VEGF. Percentage of immuno‐positive cell count for ERα was done. Histoenzymic NADPH‐d expression was analysed. Expression of all these factors increased during follicular phase of oestrous cycle in order to support the angiogenesis; however, the expression was significantly lower (p ≤ 0.05) in term of OD value as well as percentage count of immuno‐positive cells during summer season indicating lower angiogenic activity that subsequently affected reproduction in buffalo. [ABSTRACT FROM AUTHOR]

Published
2024
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