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2 results on '"Patrizia De Matteo"'

1. Avian Pneumovirus infection in turkey and broiler farms in Italy: a virological, molecular and serological field survey

Author

Clive John Naylor, Maria Alessandra De Marco, Carlo Franciosi, Giovanni Ortali, Patrizia De Matteo, Mauro Delogu, and Mattia Cecchinato

Subjects
Turkey Rhinotracheitis (TRT), Avian Pneumovirus (APV), Turkeys, Broilers, Reverse transcriptase - Polymerase chain reaction (RT-PCR), Animal culture, SF1-1100
Abstract

Avian Pneumovirus (APV) is the casual agent of Turkey Rhinotracheitis (TRT), and also causes a respiratory infection inchickens, which can result in Swollen Head Syndrome. A survey of APV infection in Italian turkey and broiler farms, in ahighly populated area of Northern Italy (Verona Province) is reported. Nine turkey farms and 6 broiler farms were sampled.Sixteen birds from each group were doubly swabbed from the choanal cleft for virus isolation on tracheal organ cultures(TOC) and RT nested PCR (A and B type specific) using extracted RNA. At the same time blood samples were collectedfor a blocking ELISA serological assay. The broiler samples for virological assays were treated with infectious bronchitisvirus (IBV) antiserum raised against serotypes prevalent in the areas sampled, thus avoiding competitive growthof IBV on TOC. Ciliostasis on TOC was taken as the indicator of the presence of the virus, and confirmation was by indirectimmunofluorescence. APV was isolated and detected by RT-PCR in 19 day-old turkeys, and in 34, 42 and 48 day-oldbroilers. All APV strains were found to be type B. All turkeys of more than 4 weeks old were APV positive by ELISA. APVinfection was found to be widely spread in the area sampled and the protocol used for virus isolation was shown to beeffective. Turkey rhinotracheitis first appeared in Italy in the late 1980s, and the APV isolates involved were subsequentlycharacterised as B types. Our results confirm APV B type to be present in Italy, but the limited findings to date need tobe extended by further surveys of other Italian regions.

Published
2010
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2. Evidenze sperimentali della resistenza del Piccione (Columba Livia) all’infezione da Metapneumovirus aviare e della sua irrilevanza nella trasmissione dell’infezione al tacchino

Author

CATELLI, ELENA, LUPINI, CATERINA, LISTORTI, VALERIA, DE MATTEO, PATRIZIA, Alberto Marziali, Clive J. Naylor, Mattia Cecchinato, SOCIETÀ ITALIANA DI PATOLOGIA AVIARE, Elena Catelli, Caterina Lupini, Valeria Listorti, Alberto Marziali, Patrizia De Matteo, Clive J. Naylor, and Mattia Cecchinato

Subjects
AVIAN METAPNEUMOVIRUS, PICCIONE, TACCHINO
Abstract

Avian metapneumovirus (AMPV) causes an upper respiratory tract infection in turkeys leading to turkey rhinotracheitis. In other avian species, including chickens, it is also involved in the etiology of multifactorial diseases such as swollen head syndrome. Sensitivity of wild birds to AMPV and their role in maintaining and spreading the virus to poultry is still a matter of debate. Recently the sensitivity of pigeons to AMPV of subtype A or B has been claimed, based on very limited PCR detections from wild or experimentally infected birds. In order to have conclusive evidence regarding the sensitivity of pigeons to AMPV of subtype B and its role in spreading the virus to turkeys, two experimental trials were planned in secure isolation conditions. In trial 1 two isolators were modified to host turkeys and pigeons in the same environment, separated only by a net. Drinkers were shared between groups. Pigeons were infected with AMPV and housed in one isolator with naïve turkeys. Similarly turkeys were infected and housed with naïve pigeons. Additional turkeys and pigeons were kept in different isolators as uninfected controls. Post-infection clinical signs, virus shedding and immune response were assessed for three weeks. In trial 2, commercial two-weeks old turkeys were divided in two groups and housed in two different isolators. Birds in isolator A were challenged as previously described. Four days post-infection, 7 weeks old naïve pigeons were introduced in the isolator A and kept with the infected turkeys for 24 hours, then removed, sprayed with 0,5 % of Wirkon S® solution. After 10 minutes, pigeons were rinsed with water, dried, and introduced in the isolator B, where 10 naïve turkeys were housed. Clinical sign were monitored for 10 days. Pigeons were found refractory to AMPV experimental infection and neither able to spread the virus to naïve turkeys. Our paper shows that pigeons are highly unlikely to play any relevant role in the environmental spread of subtype B AMPV. Pigeons are not biological vector or reservoir species for AMPV subtype B.

Published
2012

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