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3 results on '"Paul Stanescu"'

1. In Vitro Studies of Bacterial Cellulose and Magnetic Nanoparticles Smart Nanocomposites for Efficient Chronic Wounds Healing

Author

Bianca Galateanu, Mihaela-Cristina Bunea, Paul Stanescu, Eugenia Vasile, Angela Casarica, Horia Iovu, Anca Hermenean, Catalin Zaharia, and Marieta Costache

Subjects
Internal medicine, RC31-1245
Abstract

The quality of life of patients with chronic wounds can be extremely poor and, therefore, over the past decades, great efforts have been made to develop efficient strategies to improve the healing process and the social impact associated with these conditions. Cell based therapy, as a modern tissue engineering strategy, involves the design of 3D cell-scaffold bioconstructs obtained by preseeding drug loaded scaffolds with undifferentiated cells in order to achieve in situ functional de novo tissue. This paper reports on the development of bionanocomposites based on bacterial cellulose and magnetic nanoparticles (magnetite) for efficient chronic wounds healing. Composites were obtained directly in the cellulose bacterial culture medium by dispersing various amounts of magnetite nanoparticles during the biosynthesis process. After purification and drying, the membranes were characterized by Raman spectroscopy and X-ray diffraction to reveal the presence of magnetite within the bacterial cellulose matrix. Morphological investigation was employed through SEM and TEM analyses on bionanocomposites. The biocompatibility of these innovative materials was studied in relation to human adipose derived stem cells in terms of cellular morphology, viability, and proliferation as well as scaffolds cytotoxic potential.

Published
2015
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2. Nanofibrous scaffolds based on bacterial cellulose crosslinked with oxidized sucrose

Author

Denis Mihaela Panaitescu, Sergiu Stoian, Adriana Nicoleta Frone, George Mihai Vlăsceanu, Dora Domnica Baciu, Augusta Raluca Gabor, Cristian Andi Nicolae, Valentin Radiţoiu, Elvira Alexandrescu, Angela Căşărică, Celina Damian, and Paul Stanescu

Subjects
Sucrose, Tissue Scaffolds, Tissue Engineering, Structural Biology, Nanofibers, Biocompatible Materials, General Medicine, Cellulose, Molecular Biology, Biochemistry, Porosity
Abstract

In this work, oxidized sucrose (OS), which is a safe bio-based and non-toxic polyaldehyde, was used as a crosslinker in defibrillated bacterial cellulose (BC) sponges obtained by freeze-drying. For mimicking the proteins' crosslinking, BC was first modified with an aminosilane to partially replace the OH groups on the BC surface with more reactive amino groups. Further, the aminosilane-grafted bacterial cellulose (BCA) was crosslinked with OS in different concentrations and thermally cured. Functionalized bacterial celluloses showed a good thermal stability, comparable to that of unmodified cellulose and much improved mechanical properties. A threefold increase in the compression strength was obtained for the BCA scaffold after crosslinking and curing. This was correlated with the uniform pore structure emphasized by the micro-CT and SEM analyses. The OS-crosslinked BCA scaffolds were not cytotoxic and showed a porosity of around 80 %, which was almost 100 % open porosity. This study shows that the crosslinking of aminated BC scaffolds with OS allows the obtaining of 3D cellulose structures with good mechanical properties and high porosity, suitable for soft tissue engineering. The results recommend this new method as an innovative approach to obtaining biomaterial scaffolds that mimic the natural extracellular matrix.

Published
2022

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