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4 results on '"Peixoto, Rafael José Marques"'

1. Antarctic strict anaerobic microbiota from Deschampsia antarctica vascular plants rhizosphere reveals high ecology and biotechnology relevance

Author

Peixoto, Rafael José Marques, Miranda, Karla Rodrigues, Lobo, Leandro Araujo, Granato, Alessandra, de Carvalho Maalouf, Pedro, de Jesus, Hugo Emiliano, Rachid, Caio T. C. C., Moraes, Saulo Roni, dos Santos, Henrique Fragoso, Peixoto, Raquel Silva, Rosado, Alexandre Soares, and Domingues, Regina Maria Cavalcanti Pilotto

Published
2016
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3. Production of AI-2 is mediated by the S-ribosylhomocystein lyase gene lux S in Bacteroides fragilis and Bacteroides vulgatus.

Author

Peixoto, Rafael José Marques, Miranda, Karla Rodrigues, Ferreira, Eliane Oliveira, de Paula, Geraldo Renato, Rocha, Edson Ribeiro, Lobo, Leandro Araujo, and Domingues, Regina Maria Cavalcanti Pilotto

Subjects
BACTEROIDES fragilis, ESCHERICHIA coli, QUORUM sensing, GRAM-negative bacteria, POLYMERASE chain reaction
Abstract

Quorum sensing is a cell-cell signaling mechanism based on cell density and that involves the production of hormone-like molecules called autoinducers (AI). One of the most studied AIs has been termed AI-2, and its biosynthesis requires the enzyme encoded by luxS. We have previously described for the first time that Bacteroides species can produce molecules with AI-2 activity. In this study, we focus on the detection of luxS and its activity as the AI-2 synthase in Bacteroides species. The strains Bacteroides fragilis B3b and Bacteroides vulgatus ATCC 8482 were selected based on a positive phenotype for AI-2 production and the presence of a putative luxS in the genome, respectively. In order to identify the luxS gene, cloning and heterologous expression strategies were utilized. We demonstrate that both strains contain functional luxS orthologs that can complement AI-2 production in Escherichia coli. [ABSTRACT FROM AUTHOR]

Published
2014
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4. Production of AI-2 is mediated by the S-ribosylhomocystein lyase gene luxS in Bacteroides fragilis and Bacteroides vulgatus.

Author

Peixoto RJ, Miranda KR, Ferreira EO, de Paula GR, Rocha ER, Lobo LA, and Domingues RM

Subjects
Amino Acid Sequence, Bacterial Proteins metabolism, Bacteroides metabolism, Bacteroides fragilis metabolism, Carbon-Sulfur Lyases metabolism, Conserved Sequence, Escherichia coli genetics, Escherichia coli metabolism, Genetic Complementation Test, Homoserine biosynthesis, Lactones, Molecular Sequence Data, Quorum Sensing, Recombinant Proteins genetics, Recombinant Proteins metabolism, Sequence Alignment, Signal Transduction, Bacterial Proteins genetics, Bacteroides genetics, Bacteroides fragilis genetics, Biofilms growth & development, Carbon-Sulfur Lyases genetics, Gene Expression Regulation, Bacterial, Homoserine analogs & derivatives
Abstract

Quorum sensing is a cell-cell signaling mechanism based on cell density and that involves the production of hormone-like molecules called autoinducers (AI). One of the most studied AIs has been termed AI-2, and its biosynthesis requires the enzyme encoded by luxS. We have previously described for the first time that Bacteroides species can produce molecules with AI-2 activity. In this study, we focus on the detection of luxS and its activity as the AI-2 synthase in Bacteroides species. The strains Bacteroides fragilis B3b and Bacteroides vulgatus ATCC 8482 were selected based on a positive phenotype for AI-2 production and the presence of a putative luxS in the genome, respectively. In order to identify the luxS gene, cloning and heterologous expression strategies were utilized. We demonstrate that both strains contain functional luxS orthologs that can complement AI-2 production in Escherichia coli., (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2014
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