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The experiment aimed to establish a method for quantitatively measuring the concentration of bacterial endotoxins in cat food using the dynamic turbidity method. Bacterial endotoxins in cat food samples were extracted with water, centrifuged, filtered, and the filtrate was appropriately diluted. Quantitative determination was carried out using the dynamic turbidity method on a bacterial endotoxin measuring instrument. The results showed good linearity of bacterial endotoxin within the range of 0.040 to 5.000 EU/mL (correlation coefficient r=-0.995 3, >0.980). The standard curve was effective, and the relative standard deviation (RSD) of repeated measurements ranged from 8.9% to 14.0% ( n=6). The recovery rates of interference tests were between 50% and 200% when the dilution factor was ≥1 000. The limit of quantification (LOQ) of the method was determined as 0.04 EU/mg. The study indicates that the method is simple, reliable, accurate, and exhibits good reproducibility, making it effective for detecting bacterial endotoxin content in cat food samples, with promising application prospects. [ABSTRACT FROM AUTHOR]

Background: The aim of this study was to describe the epidemiology of prelabour rupture of membranes (PROM) in China and to assess the association between clinical practice following the guidelines and early neonatal infections., Methods: We conducted a prospective cohort study of 15926 deliveries in ShenZhen Baoan Women's and Children's Hospital, Xibei Women's and Children's Hospital and Chengdu Women's and Children's Hospital between August 1, 2017, to March 31, 2018. Clinical data were collected for each participant. The epidemiology of PROM was described. The association between PROM with early neonatal infectious outcomes and the influence of the implementation of the guideline on early neonatal infectious outcomes were assessed., Findings: The incidence of PROM was 18•7%. PROM was showed to be a risk factor for neonatal infectious diseases (adjusted OR 1•92, 95%CI 1•49~2•49, p <0•0001), early-onset pneumonia (EOP) (adjusted OR 1•81, 95%CI 1•29~2•53, p =0•0006) and early-onset sepsis(EOS) (adjusted OR 14•56, 95%CI 1•90~111•67, p =0•01) for term neonates. For term neonates born from mother with PROM, induction of labor according to the guideline was a protective factor for neonatal diseases(adjusted OR 0•50, 95%CI 0•25~1•00, p =0•00498) and EOP(adjusted OR 0•32, 95%CI 0•11~0•91, p =0•03). For preterm neonates born from mother with PROM, using antibiotics according to the guideline showed to be protective for neonatal infectious diseases (adjusted OR 0•14, 95%CI 0•09~0•23, p <0•0001) and EOP (adjusted OR 0•08, 95%CI 0•04~0•14, p <0•0001)., Interpretation: Our study showed the risk of PROM for infectious diseases (including EOP and EOS) and the benefit of the usage of antibiotics according to the guideline for infectious diseases and EOP for preterm neonates., Funding: National Natural Science Foundation of China, Capital Medical Development Research Fund of Beijing., Competing Interests: We declare no competing interests., (© 2020 The Authors. Published by Elsevier Ltd.)